Progress 03/01/19 to 02/29/24
Outputs
(N/A)
Impacts
What was accomplished under these goals?
Aim 1. Determine in vitro, the mechanisms mediating anti-inflammatory effects of long chain ω-3 PUFA and tart cherry individually and in combination in adipocytes and macrophages. We have completed the proposed cell culture experiments where we treated cultured murine adipocytes with tart cherry, fatty acids or their combinations at various doses. To test whether these compounds reduce or prevent lipopolysaccharide (LPS, inflammatory stimulus) induced inflammation in adipocytes. We have also conducted various measurements of outcomes, including adipocyte secreted cytokines (measured by ELISA or multiplex immunoassays) , changes in adipocyte proteins (determined by proteomics/mass spectrometry). We found that tart cherry and/or omega 3 fatty acid (specifically eicosapentaenoic acid, EPA) reduced interleukin 6 and monocyte chemoattractant protein secretion by LPS-stimulated adipocytes and both compounds also increased expression of respiratory chain proteins indicating increased mitochondrial function especially in present of tart cherry extracts. We further confirmed these findings using western blotting where we validated changes in the mitochondrial proteins and also demonstrated that tart cherry, EPA and combined compounds significantly reduced p65 NFK-B subunit phosphorylation. Interestingly, lower doses of the combined EPA and tart cherry were more effective than high doses of these compounds (where in fact inflammation was aggravated). We are currently drafting manuscript reporting these data for near future publication. Aim 2. Determine in vivo, the effects of fish oil and tart cherry individually and in combination on obesity-associated inflammation in diet-induced obese mice. This aim was already completed for the most part and part of it published and presented at several; scientific conferences, including the American Society for Nutrition (ASN) and the Obesity Society. Two more manuscripts are being drafted. One to be resubmitted this summer where we report changes in inflammatory, autophagy and ER stress genes and proteins in response to tart cherry and EPA, namely significant changes were observed in adipose tissues and less in livers from these mice. We also report that major changes were observed in female mice, both B6 diet induced obese mice as well as polygenic model Tally Ho inducted obese mice. Lastly, while we have not originally proposed microbiome studies in this proposal, we have conducted 16S sequencing of fecal samples from these mice and completed these analyses recently, which were presented at the ASN conference, Nutrition 2024, for which the student on this project was Finalist at the Graduate Student Research Competition (top 8 student awards). In this work, we demonstrated that while we did not identify significant synergistic or addictive effects of tart cherry and EPA for all metabolic outcomes, there were positive changes in gut microbiota composition and beta as well as alpha diversity in mice receiving both tart cherry and fish oil compared to individual compounds or high fat control diets.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2023
Citation:
MARYAM SEIFISHAHPAR, KALHARA MENIKDIWELA, SHANE SCOGGIN, JUNG HAN KIM, NAIMA MOUSTAIDMOUSSA.
Tart Cherry and Fish Oil Effects in Male and Female Diet-induced (C57B6/6J) and Genetically Obese
(TALLYHO/Jng) Mice. Nutrition 2024, Annual meeting of the American Society for Nutrition, Chicago, 2024. Published in Curr. Dev Nutr. Suppl.2024. Awarded top 8 finalist in graduate research presentation at ASN
Maryam Seifishahpar, Kalhara Menikdiwela, Joao Pedro Torres Guimaraes, Shane Scoggin, Jung Han Kim, Halima Bensmail, Amal Awadalla Mohamed Ali, Naima Moustaid-Moussa.
Effects of Tart Cherry and Fish Oil on inflammation and gut microbiota composition in Diet-induced (B6) and Genetically Obese (TALLYHO/Jng) Male and Female Mice. Nutrition 2023, Annual meeting of the American Society for Nutrition, Boston, 2023. Published in Curr. Dev Nutr. Suppl.2023
Maryam Seifishahpar, Kalhara Menikdiwela, Shane Scoggin, Jung Han Kim, Micah Castillo, Naima Moustaid-Moussa.
Effect of High Fat Diet on Inflammation and Gut Microbiota Composition in Diet-Induced (B6) and Genetically (TALLYHO/Jng) Obese Mice Models. FASEB Science Research Conference in Nutritional Immunology, Melbourne, FL. 2023
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Progress 03/01/23 to 02/29/24
Outputs
Target Audience:Students (undergraduate and graduate), postdocs, scientists working in nutrition and chronic disease areas, and the public interested in effects of diet on health Changes/Problems:None What opportunities for training and professional development has the project provided?The peer mentoring we used for this project and overall, in our lab, proved to be very beneficial for both mentees (undergraduate and graduate students) and mentors (graduate students and postdocs, and some senior undergraduates). All trainees received lab specific trainings as well as hands on trainings and other trainings through lab meetings, and were all required to complete the training in Responsible Conduct for Research, compliances, as well as animal and cell culture studies and writing research and review papers. Despite the delay in experimentation over year 1 and part of year 2, due to COVID 19, we have made significant progress, and completed all proposed work and beyond, pending now publication of our last 2 or 3 papers. Undergraduate and graduate students presented at local and national conferences Students had the opportunity to participate and learn about community outreach and engagement through other ongoing projects with Texas A&M AgriLife Extension How have the results been disseminated to communities of interest?We disseminated our results through several local (Texas Tech graduate and undergraduate conferences and annual meeting of the Obesity Research Institute, ORI) and national presentations at national meetings (ASN and TOS) as well as FASEB summer conferences. Moreover, findings from our research was shared with undergraduate and graduate students in classes and the ORI meetings and workshops by the PD, graduate or undergraduate student and other lab members involved in this project. Our findings were shared with other stakeholders such as schools with which we work and AgriLife Extension community educators, and other service activities led by the graduate nutrition student organization What do you plan to do during the next reporting period to accomplish the goals?This is the final report. However we plan to complete publication of remaining manuscripts over coming months.
Impacts
What was accomplished under these goals?
Aim 1. Determine in vitro, the mechanisms mediating anti-inflammatory effects of long chain ω-3 PUFA and tart cherry individually and in combination in adipocytes and macrophages. We have completed the proposed cell culture experiments where we treated cultured murine adipocytes with tart cherry, fatty acids or their combinations at various doses. To test whether these compounds reduce or prevent lipopolysaccharide (LPS, inflammatory stimulus) induced inflammation in adipocytes. We have also conducted various measurements of outcomes, including adipocyte secreted cytokines (measured by ELISA or multiplex immunoassays) , changes in adipocyte proteins (determined by proteomics/mass spectrometry). We found that tart cherry and/or omega 3 fatty acid (specifically eicosapentaenoic acid, EPA) reduced interleukin 6 and monocyte chemoattractant protein secretion by LPS-stimulated adipocytes and both compounds also increased expression of respiratory chain proteins indicating increased mitochondrial function especially in present of tart cherry extracts. We further confirmed these findings using western blotting where we validated changes in the mitochondrial proteins and also demonstrated that tart cherry, EPA and combined compounds significantly reduced p65 NFK-B subunit phosphorylation. Interestingly, lower doses of the combined EPA and tart cherry were more effective than high doses of these compounds (where in fact inflammation was aggravated). We are currently drafting manuscript reporting these data for near future publication. Aim 2. Determine in vivo, the effects of fish oil and tart cherry individually and in combination on obesity-associated inflammation in diet-induced obese mice.This aim was already completed for the most part and part of it published and presented at several; scientific conferences, including the American Society for Nutrition (ASN) and the Obesity Society. Two more manuscripts are being drafted. One to be resubmitted this summer where we report changes in inflammatory, autophagy and ER stress genes and proteins in response to tart cherry and EPA, namely significant changes were observed in adipose tissues and less in livers from these mice. We also report that major changes were observed in female mice, both B6 diet induced obese mice as well as polygenic model Tally Ho inducted obese mice. Lastly, while we have not originally proposed microbiome studies in this proposal, we have conducted 16S sequencing of fecal samples from these mice and completed these analyses recently, which were presented at the ASN conference, Nutrition 2024, for which the student on this project was Finalist at the Graduate Student Research Competition (top 8 student awards). In this work, we demonstrated that while we did not identify significant synergistic or addictive effects of tart cherry and EPA for all metabolic outcomes, there were positive changes in gut microbiota composition and beta as well as alpha diversity in mice receiving both tart cherry and fish oil compared to individual compounds or high fat control diets.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
MARYAM SEIFISHAHPAR, KALHARA MENIKDIWELA, SHANE SCOGGIN, JUNG HAN KIM, NAIMA MOUSTAID-MOUSSA. Tart Cherry and Fish Oil Effects in Male and Female Diet-induced (C57B6/6J) and Genetically Obese (TALLYHO/Jng) Mice. Nutrition 2024, Annual meeting of the American Society for Nutrition, Chicago, 2024. Published in Curr. Dev Nutr. Suppl.2024
Maryam Seifishahpar, Kalhara Menikdiwela, Joao Pedro Torres Guimaraes, Shane Scoggin, Jung Han Kim, Halima Bensmail, Amal Awadalla Mohamed Ali, Naima Moustaid-Moussa.
Effects of Tart Cherry and Fish Oil on inflammation and gut microbiota composition in Diet-induced (B6) and Genetically Obese (TALLYHO/Jng) Male and Female Mice. Nutrition 2023, Annual meeting of the American Society for Nutrition, Boston, 2023. Published in Curr. Dev Nutr. Suppl.2023
Maryam Seifishahpar, Kalhara Menikdiwela, Shane Scoggin, Jung Han Kim, Micah Castillo, Naima Moustaid-Moussa. Effect of High Fat Diet on Inflammation and Gut Microbiota Composition in Diet-Induced (B6) and Genetically (TALLYHO/Jng) Obese Mice Models. FASEB Science Research Conference in Nutritional Immunology, Melbourne, FL. 2023
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Progress 03/01/22 to 02/28/23
Outputs
Target Audience:Students (undergraduate and graduate), postdocs, scientists working in nutrition and chronic disease areas,and the public interested in effects of diet on health Changes/Problems:Past year, we did not have any major challenges, previous delays and requests for NCEwere due to delays in starting the project due to student visa issues then an instrument failure, and other delays related to the pandemic. All were addressed and project has progressed well over the past couple years. What opportunities for training and professional development has the project provided?The peer mentoring we used for this project and overall in our lab, proved to be very beneficial for both mentees (undergraduate and graduate stduents) and mentors (graduate students and postdocs, and some senior undergraduates). All trainees received lab specific trainings as well as hands on trainings and otehr trainings through lab meetings, and were all required to complete the training in Responsible Conduct for Research, compliances, as well as animal and cell culture studies and writing research and review papers. Despite the delay in experimentation over year 1 and part of year 2, we have made significant progress, and all personnel has been in place over the past year and half and making progress in the project as described above. Undergraduate and graduate students presented at local and national conferences Students had the opportunity to participate and learn about community outreach and engagement through other ongoing projects with Texas A&M AgriLife Extension How have the results been disseminated to communities of interest?We disseminated our results through several local (Texas Tech graduate and undergraduate conferences and annual meeting of the Obesity Reserach Institute, ORI) and national presentations at national meetings (ASN and TOS). The local presentation included in fall 2022 a seminar presentation as part of the Sustainable Agriculture Seminar Series organized by the ORI. Remaining manuscripts from this work will be prepared for submission to peer reviewed journals this year and next. Moreover, findings from our research was shared with undergraduate and graduate students in classes and the ORI meetings and workshops by the PD, graduate or undergraduate student and other lab members involved in this project. Our findings wereshared with other stakeholders such as schools with which we work and AgriLife Extension community educators, and other service activties led by the graduate nutrition stduent organization What do you plan to do during the next reporting period to accomplish the goals?Next report will be final report. 1. We anticipate completing all proposed work plus additional data on changes in mcirobiota by the dietary interventions (not originally proposed). 2. We anticipate submitting the remaining manuscripts- two peer review full reserach papers anticipated plus 1 review paper, all currently in initial draft stages
Impacts
What was accomplished under these goals?
Aim 1. Determine in vitro, the mechanisms mediating anti-inflammatory effects of long chain ω-3 PUFA and tart cherry individually and in combination in adipocytes and macrophages. We are finalizing the cell culture experiments with tart cherry and fatty acids in adipocytes, in fatty acid- or lipopolysaccharide stimulated cells (to simulate obesity-associated inflammation). Further, we have been validating identified target proteins from our mass spectrometry/proteomic analyses of tart cherry and/or omega 3 fatty acid treated adipocytes. Proteins identified that were regulated by these bioactive compounds includedmitochondrial respiratory chain proteins and proteins involved in inflammatory processes and protein translation. Once the cell culture experiments and the protein validation is complete, we will combine these data into a manuscript next year. Aim 2. Determine in vivo, the effects of fish oil and tart cherry individually and in combination on obesity-associated inflammation in diet-induced obese mice. Most of this aim has been completed and one peer reviewed paper published, and several abstracts presented over the past year at internal and national conferences. We are finalizing the second manuscript focusing on protein and gene analyses in adipose tissues and livers from these mice, to determine key metabolic and signaling pathways altered by tart cherry and fish oil. Key target pathways identified include inflammation, autophagy and ER stress with sex and genotype (diet induced vs genetically obese TH mice). All analyses of gene expression by real time PCR have been completed, we also recently completed all protein analyses by western blotting and data are being analyzed and written up. In addition, although not originally proposed under Nutrient Efficacy program, we collected fecal samples from our mice, and we are also conducting microbiota analyses that may generate new hypotheses for a future project.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Seif, M., et al.. Genotype and Sex Differences in Inflammation and Gut Microbiota Composition in Diet-induced Obese Mice. ObesityWeek (Abstract) annual scientific meeting of The Obesity Society, November 2022
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Seif, M., et al., Tart Cherry and Fish Oil Effects in Male and Female Diet-induced (C57B6/6J) and Genetically Obese (TALLYHO/Jng) Mice. Curr. Dev. Nutr (Abstract), June 2022, Nutrition 2022, Annual Scientific Meeting of The American Society for Nutrition
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Seif, M., et al. Effects of Supplemental Tart Cherry and Fish Oil on Inflammation in Diet-induced and Genetically Obese Mice. Presented at the Texas Tech Graduate School Research Symposium (March 2022), The Obesity Research Institute (May 2022); and the 3MT Thesis/Dissertation Competition (October 2022), Texas Tech, 2022
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Progress 03/01/21 to 02/28/22
Outputs
Target Audience:Students (undergraduate and graduate), postdocs, nutrition and chronic disease scientists and public interested in effects of diet on health Changes/Problems:We mainly encountered major delays due to the pandemic at the beginning of the project. All personnel and experimentation are progressing as planned at this time and a first year NCE has been approved.. A second NCE may be requested to finalize last experiments and publish remaining work What opportunities for training and professional development has the project provided?Graduate and undergraduate students and postdocs (serving as co-mentors for undergraduate and graduate students) have been involved in this project and received training in Responsible Conduct for Research, compliances, as well as animal and cell culture studies and writing research and review papers. Despite the delay in experimentation over year 1 and part of year 2, we have made significant progress, and all personnel has been in place over the past year and half and making progress in the project as described above. How have the results been disseminated to communities of interest?Research papers have been published on tart cherry and fish oil by our team. Some of the research was submitted to scientific conferences, including an abstract recently submitted to the American Society for Nutrition (Nutrition2022). We also reported our findings in 2021 during the USDA PD virtual meeting. Manuscripts related to ongoing experiments will be prepared for submission to peer reviewed journals this coming year. Moreover, findings from our research was also shared with undergraduate and graduate students in classes thought by the PD or her graduate students, research faculty or postdocs, and also with other stakeholders within the university and in the community (schools during outreach activities). What do you plan to do during the next reporting period to accomplish the goals?We will finalize the cell culture studies with omega 3 fatty acids and tart cherry treatments of adipocytes, and determine changes in gene and protein expressions. In addition, we will continue analyses of tissues collected form the animal studies, especially liver and adipose tissue to determine mechanisms mediating observed metabolic outcomes following the various dietary interventions. We anticipate submission of 1-2 research paper over the next year. As indicated above, we encountered delays due to the pandemic in the first year and part of year 2. We are grateful that the no cost extension was approved, and we may request one more no cost extension to finalize reporting and final manuscript submissions.
Impacts
What was accomplished under these goals?
Aim 1. Determine in vitro, the mechanisms mediating anti-inflammatory effects of long chain ω-3 PUFA and tart cherry individually and in combination in adipocytes and macrophages. These experiments are still ongoing. We have conducted dose response studies in adipocytes and demonstrated that tart cherry dose-dependently reduced LPS-induced inflammation in differentiated adipocytes, but smaller effects were observed in macrophages, thus we primarily focused on adipocytes. Further, we identified in proteomic analyses of adipocytes treated with tart cherry, eicosapentaenoic acid (EPA) or combination of both that several proteins involved in TCA cycle and oxidative phosphorylation and mitochondrial function are increased by TC, while EPA primarily reduced inflammatory proteins, known to be transcriptionally regulated by the NFKB pathway. These data will be combined with tissue gene and protein analyses from adipose tissue from aim 2 below to submit as a second research paper. Aim 2. Determine in vivo, the effects of fish oil and tart cherry individually and in combination on obesity-associated inflammation in diet-induced obese mice. We have already published animal studies reporting sex- and genotype specific differences in response to tart cherry or fish oil, using both diet-induced obesity and a genetic model of obesity (Tally Ho , TH mice). Since publication of this paper early 2021 in the Journal of Nutritional Biochemistry, we have conducted further analyses of adipose tissue from these mice, to understand mechanisms underlying in vivo metabolic responses. Data obtained thus far demonstrate that in diet-induced obese mice, it is primarily the inflammatory pathways that are reduced by tart cherry and/or fish oil; this is based on qRT-PCR gene expression analyses, further validation using protein analyses is ongoing. Surprisingly, in the TH genetic model, we did not see major changes in these inflammatory mechanisms, despite improved glucose tolerance, and reduced circulating IL6 levels, especially with fish oil. We plan in coming year to complete gene and protein expression analyses from adipose tissue and liver form these mice, and also combine in coming year data from these animal studies with findings in cultured adipocytes from aim 1, into another research paper.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
164. Parkman, J.K., Sklioutovskaya-Lopez, K., Menikdiwela, K.R., Freeman, L., Moustaid-Moussa, N., Kim, J.H. Effects of high fat diets and supplemental tart cherry and fish oil on obesity and type 2 diabetes in male and female C57BL/6J and TALLYHO/Jng mice. J. Nutr. Biochem. 94:108644; 2021
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Progress 03/01/20 to 02/28/21
Outputs
Target Audience:Students (undergraduate and graduate), postdocs, nutrition and chronic disease scientists and public interested in effects of diet on health Changes/Problems:As indicated in previous page, we encountered delays due to the pandemic, including lockdown and limited personnel in labs. Therefore, we will likely require no cost extensions for this project, which will be requested in 2022. What opportunities for training and professional development has the project provided?Graduate and undergraduate students have been involved in this project and received training in Responsible Conduct for Research, compliances, as well as animal and cell culture studies and writing research and review papers. Unfortunately, due to restrictions on international student admissions this past year, and lockdown due to COVID, student experiences were less than anticipated, experimentally, and the graduate student that was primarily assigned to this project was not able to join in summer 2020. However, students and postdocs working on this project utilized this time to advance their literature research and planning for future experiments, while learning new techniques in part virtually. How have the results been disseminated to communities of interest?Research papers have been publishedon tart cherry and fish oil by our team, and one paper is pending publication. Some of the research was submitted to scientific conferences, however due to the pandemic, face to face meetings were cancelled. Manuscripts related to ongoing experiments will be prepared for submission to peer reviewed journals this coming year. Moreover, findings from our research was also shared with undergraduate and graduate students in classes thought by the PD or her graduate students, research faculty or postdocs, and also with other stakeholders within the university and in the community (schools during outreach activities). What do you plan to do during the next reporting period to accomplish the goals?We will continue the cell culture studies with omega 3 fatty acids and tart cherry treatments of adipocytes, and determine changes in gene and proteinexpressions. In addition, we will continue analyses of tissues collected form the animal studies, espcially liver and adipose tissue to determine mechanisms mediating observed metabolic outcomes following the variousdietary interventions. We anticipate submission of 1-2 research paper over the next year. As indicated above, we encountered delays due to the pandemic, includinglockdown and limited personnel able to access labs, as well as inability of the international doctoral student assigned to this project to obtain a visa and join our lab. She will now join in summer 2021.We will likely require no cost extensions for this project, which will be requested next year.
Impacts
What was accomplished under these goals?
Aim 1. Determine in vitro, the mechanisms mediating anti-inflammatory effects of long chain ω-3 PUFA and tart cherry individually and in combination in adipocytes and macrophages. We have conducted further dose response studies both in adipocytes and macrophages. As expected tart cherry dose-dependently reduced LPS-induced inflammation in differentiated adipocytes, but smaller effects were observed in macrophages. Further, we conducted proteomic studies using individual and combined doses of tart cherry and omega 3 fatty acids. Several proteins were differentially expressed among various treatments. Notably, in adipocytes, several mitochondrial function-related proteins were upregulated by tart cherry, in addition to reduced proteins related to inflammatory pathways. Similarly, many of these same inflammatory pathways were also reduced by eicosapentaenoic acid, which also downregulated metabolic pathways related to n-6 PUFA. Additional analyses are ongoing to complete these experiments and write them up into a research manuscript to be submitted this coming year. Aim 2. Determine in vivo, the effects of fish oil and tart cherry individually and in combination on obesity-associated inflammation in diet-induced obese mice. We have completed an animal study where we tested individual vs. combined fish oil and tart cherry effects in both lean and obese mice fed low fat or high fat diets with and without the dietary bioactive compounds; using both B6 and genetically obese TH mice. No preventive effects of tart cherry (TC) or fish oil (FO) or their combination were observed in HF-induced adiposity or energy imbalance, but FO significantly reduced glucose intolerance in TH males compared to non control non supplemented groups. Further, both TC and FO decreased plasma IL6 levels, especially in females, without additive or synergistic effects of these two. Collectively, sexually dimorphic effects of dietary supplementation were observed for glucose metabolism and inflammatory markers. A manuscript has been submitted for these findings to the Journal of Nutritional Biochemistry. Once we complete analyses of available tissues form past year animal study, we will plan accordingly the next animal experiments with different doses of TC and FO in diet-induced obese mice.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Harlan, L., Mena, L.T., Ramalingam, L., Jayarathne, S., Shen, C-L., Moustaid-Moussa, N. Mechanisms Mediating Anti-Inflammatory Effects of Delta-Tocotrienol and Tart Cherry Anthocyanins in 3T3-L1 Adipocytes. Nutrients; 2(11):3356. doi: 10.3390/nu12113356
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Jayarathne, S., Ramalingam, L., Edwards, H., Vanapalli, S.E., Moustaid-Moussa, N. Tart Cherry Increases Lifespan in Caenorhabditis elegans by Altering Metabolic Signaling Pathways; doi: 10.3390/nu12051482. Nutrients; 12(5):1482, 2020.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Bruno RS, Neilson AP, Lambert JD, Moustaid-Moussa N. Polyphenols, obesity, and cardiometabolic health (Editorial, Special Issue). J Nutr Biochem. 89:108565, 2021. doi: 10.1016/j.jnutbio.2020.108565
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Progress 03/01/19 to 02/29/20
Outputs
Target Audience:Students (undergraduate and graduate), postdocs, nutrition and chronic disease scientists and public interested in effects of diet on health Changes/Problems:No major changes What opportunities for training and professional development has the project provided?a graduate and undergraduate students have been involved in this project and received training in Responsible Conduct for Research, Compliances, as well as animal and cell culture studies. How have the results been disseminated to communities of interest?A review paper on anthocyanins and it potential inflammation reducing effects on microbiome has been published. Experimental data are beeing analzyed and will be submitted for publication this coming year. What do you plan to do during the next reporting period to accomplish the goals?As indicated above, we will continue the cell culture studies with omega 3 fatty acids and tart cherry and determine changes in proteomics and gene expression. In addition, we will continue analyses of tissues recently collected form the animal studies and based on findings design the next animal study. We anticipate at 1-2 research paper submissions over the next year.
Impacts
What was accomplished under these goals?
Aim 1. Determine in vitro, the mechanisms mediating anti-inflammatory effects of long chain ω-3 PUFA and ACY individually and in combination in adipocytes and macrophages. We have conducted dose response studies both in adipocytes and macrophage. As expected tart cherry diose-dependently reduced inflammation in differentiated adipocytes. Surprisingly, no significant effects of tart cherry were observed in cultured RAW macrophages using LPS-induced inflammation as conducted in adipocytes. Additional experiments are ongoing to determine the basis for these interesting differences as well as additional mechanistic proteomics and gene expression studies in adipocytes. Aim 2. Determine in vivo, the effects of ω-3 PUFA and ACY individually and in combination on obesity-associated inflammation in diet-induced obese mice. We have conducted an initial animal study with individual and combined omega 3 fatty acids and tart cherry and analyses are ongoing.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Jayarathne, S., Stull, AJ., Park, OJ., Kim, JH., Thompson, L., Moustaid-Moussa, N. Protective effects of anthocyanins in obesity-associated inflammation and changes in gut microbiome. Mol Nutr. Food Res.
Mol Nutr Food Res. 2019 Oct;63(20):e1900149. doi: 10.1002/mnfr.201900149. PMID: 31389663
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