Source: KANSAS STATE UNIV submitted to NRP
THE SCIENCE AND ENGINEERING FOR A BIOBASED INDUSTRY AND ECONOMY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1018193
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
S-OLD 1075
Project Start Date
Nov 6, 2018
Project End Date
Sep 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
KANSAS STATE UNIV
(N/A)
MANHATTAN,KS 66506
Performing Department
Grain Science & Industry
Non Technical Summary
The project will focus on research and development of technical feasible, economically viable and environmentally sustainable technologies to convert biomass resources into chemicals, energy, and materials in a biorefinery,
Animal Health Component
40%
Research Effort Categories
Basic
40%
Applied
40%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
51118992020100%
Knowledge Area
511 - New and Improved Non-Food Products and Processes;

Subject Of Investigation
1899 - Oilseed and oil crops, general/other;

Field Of Science
2020 - Engineering;
Goals / Objectives
Research and develop technically feasible, economically viable and environmentally sustainable technologies to convert biomass resources into chemicals, energy, materials in a biorefinery methodology including developing co-products to enable greater commercialization potential.
Project Methods
Structural characterization of lignin-protein adhesives: The prepared lignin-protein adhesive will be thoroughly characterized to study the 2D polymeric structure: SDS-PAGE will be used to determine the protein subunit and soluble protein-phenolic aggregates' molecular size, distinguishing the function of soy protein subunits to the protein-phenolic complex. Surface hydrophobicity will be used as a responsive variable to evaluate soy protein unfolding degree. Surface hydrophobicity will be evaluated by measuring SDS-binding capacity. Circular dichroism will be used to determine the protein's secondary structure conformation induced by interaction with phenolic compounds. FTIR of monomers, oligomers and cross-polymerized lignin-protein samples will be acquired to study chemical bonding. XRD patterns will be obtained for crystalline structure. The molecular weight of protein before and after reaction with lignin will be measured using a high-performance Size exclusion chromatography. The 1D and 2D 1 H-1 H NMR experiments will be performed with a 5-mm cryogenic triple-resonance inverse detection pulse field gradient probe. 2D- 1 H-1 H Total Correlation Spectroscopy and NOE Spectroscopy data will be acquired in phase-sensitive) mode into 2000 t2 and 256 t1 points with spectral width of 13 ppm in each dimension. Morphology and microstructure of lignin-protein polymers will be examined by SEM with sub-nano resolution. The curing behavior of selected adhesives will also be observed using AFM imaging.Physical and adhesion Evaluation: Rheological properties of lignin-protein cross polymerized samples will be characterized using a dynamic rheometer for shear behaver (i.e., shear thinning, shear thickening, viscosity, and viscoelastic properties) at varied shear rate and temperatures. Contact angle on wood veneer surface will be determined with an Optical Contact Angle Meter for adhesion performance evaluation. TGA-DSC will be used to determine thermal stability of lignin-protein polymers during pyrolysis. The denaturation temperature and enthalpy of soy protein glycinin and conglycinin informs protein structure/physicochemical properties. Crosslink density and entanglements are important properties to evaluate cohesiveness and curing strength, which will be determined. Water solubility and water absorption of the cured lignin-protein adhesive will also be determined and used as a responsive variable to evaluate crosslink density and degree of entanglement. Adhesion on wood will be evaluated using wood veneer following ASTM D5266-99. Lignin-protein-based adhesives prepared as described above will be applied to wood veneer, and then shear adhesion strength testing will be performed.Improvement on adhesive synthesis process. Assembling time is very important to facilitate protein molecules form uniform complex, and part of the lignin-protein polymer chain will diffuse or penetrate into the wood surface. Hot press time, press temperature, solid content, ratio of lignin to protein, and pH of adheres significantly affect adhesion quality and performance. Curing dynamics information obtained from thermal phase transition analysis of lignin-protein adhesives will be used as reference to select the range of variables. Response surface methodology will be used to study factors affecting adhesion performance of lignin-protein adhesives. Effects of these factors on dry strength, soaked strength, and water resistance will be determined by ASTM standards (D-1151-00, D1183-96) and shared with Chinese team.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Crop growers, farmers, companies will be the immediate beneficiaries of the investigation by finding new applications of soybean meals, oils and its derivatives for adhesives and coatings, agricultural feedstock processing industries, resin and biobased fuel related industries. Oilseeds and biomass related farmers and industries will also benefit from this study to find a way to utilize their products. The general public will benefit from the results, because biobased adhesives and fuels are environmentally friendly. In addition, the findings from this project will advance the bioadhesives and biofuel knowledge base and stimulate future developments within the biobased adhesive and biofuel industry. In addition, biomass and bioenergy industries will be the immediate beneficiaries of the advanced technologies for biofuel production. Changes/Problems:Dr. Yi Zheng was added to the team as a co-PI since 2018, which was not reported last year. What opportunities for training and professional development has the project provided?Students and junior researchers involved in this project gain new information and new knowledge as well as skills in the fields, which help them to obtain job opportunities in academia and industries. Senior researchers involved in this project gain new information and new knowledge that is helpful for professional promotion, recognition, and consulting. How have the results been disseminated to communities of interest?Results are disseminated to audience and communities by conference presentations, journal publications, news release, and popular magazines. What do you plan to do during the next reporting period to accomplish the goals?We will continue to conduct research on improvement of wet adhesion and thermal stability of plant based (protein, carbohydrate, oil) adhesives, and utilize lignin polymers for adhesive application, develop biological conversion of lignin into value-added bioproducts, and continue conduct research on cellulosic biofuels with focus on biomass pretreatment and improved biomass fermentation yield and efficiency.

Impacts
What was accomplished under these goals? The overall goal of this project is to develop enabling technologies that utilize agriculture feedstocks and by-products for biobased materials and bioenergy, and to generate new knowledge that can be useful reference to both academia and industries in the field. In the past year, we continued improving wet adhesion of plant proteins (soybean, cottonseeds) based adhesives modified with depolymerized lignin. We also conducted research on cellulosic biofuels with focus on biomass pretreatment and improved biomass fermentation yield and efficiency and technologies to treat and valorize the black liquor and hydroliquefaction wastewater for value-added products. We published 20 new referee articles and 5 technical conference presentations.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Liu, J. M. Wu, M. Wang, Y. Zou, Z. Tan, D. Wang, XS. Sun. 2019. Predicting the content of camelina protein using FT-IR spectroscopy coupled with SVM model. Cluster Computing (2019) 22:S8401S8406.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, Y., C. Li, XS. Sun. 2019. Fundamental Insights into the Curing Mechanisms of Epoxidized Fatty Acid Methyl Esters and Triglycerides. AOCS DOI 10.1002/aocs.12260.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, J., S. Pradyawong, Z. He, SX. Sun, D. Wang, H. Cheng, J. Zhong. 2019. Assessment and application of phosphorus/calcium-cottonseed protein adhesive for plywood production. Journal of Cleaner Production 229:454-462.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Pradyawong, S., G. Qi, X.S. Sun, D. Wang. 2019. Laccase/TEMPO-Modified Lignin Improved Soy-Protein-Based Adhesives: Adhesion Performance and Properties. International Journal of Adhesion and Adhesives 91 (2019) 116-122
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Meng, F., D. Wang. 2020. Effects of vacuum freeze-drying pretreatment on biomass and biochar properties. Renewable Energy 155 (2020) 1-9.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Zhao, J., Y. Xu, W. Wang, J. Griffin, D. Wang. 2020. Conversion of liquid hot water, acid and alkali pretreated industrial hemp biomasses to bioethanol. Bioresource Technology 309 (2020) 123383
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Zhao, J., Y. Xu, M. Zhang, D. Wang. 2020. Integrating bran starch hydrolysates with alkaline pretreated soft wheat bran to boost sugar concentration. Biorescouces Technology 302 (2020) 122826
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Li, Y., R. Kesharwani Z. Sun, R. Qin, C. Dagli, M. Zhang, D. Wang. 2020. Economic Viability and Environmental Impact Investigation for the Biofuel Supply Chain Using Co-fermentation Technology. Applied Energy 259 (2020) 114235
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, J., D. Liu, M. Zhang, H. Huang, D. Wang. 2019. Enzymatic hydrolysis and fermentation of corn stover liquor from magnesium oxide pretreatment without detoxification. Industrial Crops and Products 140 (2019) 111728
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Appiah-Nkansah, N., J. Li, W. Rooney, D. Wang. 2019. A review of sweet sorghum as a viable renewable bioenergy crop and its techno-economic analysis. Renewable Energy 143(2019) 1121-1132
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Appiah-Nkansah, N., J. Li, K. Zhang, M. Zhang, D. Wang. 2019. Study on mass transfer kinetics of sugar extraction from sweet sorghum biomass via diffusion process and ethanol yield using SSF. Processes 2019, 7(3), 137
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Sun, W., Fu, S., Zhu, R., Wang, Z., Zou, H., Zheng, Y., 2020. Improved anaerobic digestion efficiency of high-solid sewage sludge by enhanced direct interspecies electron transfer with activated carbon mediator. Bioresource Technology, 313, 123648
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Li, X., Li, M., Pu, Y., Ragauskas, A.J., Zheng, Y., 2020. Simultaneous depolymerization and fermentation of lignin into value-added products by marine protist, Thraustochytrium striatum. Algal Research, 46, 101773
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Li, X., Li, M., Pu, Y., Ragauskas, A.J., Zheng, Y., 2020. Black liquor valorization by using marine protist Thraustochytrium striatum and preliminary metabolic mechanisms study. ACS Sustainable Chemistry & Engineering, 8, 1786-1796
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ren, H., Feng, Y., Liu, T., Li, J., Wang, Z., Fu, S., Zheng, Y., Peng, Z., 2020. Effects of different simulated seasonal temperatures on the fermentation characteristics and microbial community diversities of the maize straw and cabbage waste co-ensiling system. Science of the Total Environment, 708, 135113.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Liu, R., Zhang, X., Sun, W., Zou, H., Zheng, Y., Zhang, G., Wang, Z., Fu, S., 2019. Solid-state KOH pretreatment of corn straw for anaerobic digestion: methane yield enhancement, potassium flow analysis and preliminary economic assessment. Energy & Fuels, 33, 11034-11040
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, X., Zheng, Y., 2019. Biotransformation of Lignin: Mechanisms, Applications and Future Work. Biotechnology Progress, e2922. Doi: 10.1002/btpr.2922
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ren, H., Shen, J., Pei, J., Wang, Z., Peng, Z., Fu, S., Zheng, Y., 2019. Characteristic microcrystalline cellulose extracted by combined acid and enzyme hydrolysis of sweet sorghum. Cellulose, 26, 8367-8381
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Xiao, R., Li, X., Leonard, E., Tharayil, N., Zheng, Y., 2019. Investigation on the effects of cultivation conditions, fed-batch operation, and enzymatic hydrolysate of corn stover on the astaxanthin production by Thraustochytrium striatum. Algal Research, 39, 101475
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Y. Xu, J. Li, Z. Xin, S. Bean, M. Tilley, D. Wang. 2020. Water-soluble sugars of pedigreed sorghum mutant stalks and their recovery after pretreatment. American Society of Agricultural and Biological Engineers Annual International Meeting, July 14th, 2020, virtual, United State
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Y. Yang, M. Zhang, J. Li, D. Wang. 2019. Effects of particle size on biomass pretreatment for biofuel production. Proceeding of the ASME 2019 14th International Manufacturing Science and Engineering Conference, 6/10-14, 2019, Erie, PA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: D. Wang. 2019. D. Biofuels and Biomaterials from grains and Oil Seeds. 2nd International Forum on the Science and Technology Development of Grains, Oils and Foodstuffs Zhengzhou, China, Nov. 15-17, 2019 (Invited)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: J. Li, M. Zhang, D. Wang. 2019. Biomass pretreatment by magnesium oxide for reducing sugar degradation and water consumption in biofuel production. ASABE Annual Meeting, 7/7 to 7/10, 2019, Boston MI, US. Paper No. 1900157
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Vin-Nnajiofor, M., Zheng, Y., Shi, J., 2020. Flexible Bio-Based Plastics Derived from CO2 and Organic Waste. 2020 ASABE International Meeting, Omaha, NE, USA.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ren, H., Sun, W., Wang, Z., Fu, S., Zheng, Y., Song, B., Li, Z., Peng, Z., 2020. Enhancing the enzymatic saccharification of grain stillage by combining microwave-assisted hydrothermal irradiation and fungal pretreatment. ACS Omega, 5, 12603-12614


Progress 11/06/18 to 09/30/19

Outputs
Target Audience:Crop growers, farmers, companies will be the immediate beneficiaries of the investigation by finding new applications of soybean meals, oils and its derivatives for adhesives and coatings, agricultural feedstock processing industries, resin and biobased fuel related industries. Oilseeds and biomass related farmers and industries will also benefit from this study to find a way to utilize their products. The general public will benefit from the results, because biobased adhesives and fuels are environmentally friendly. In addition, the findings from this project will advance the bioadhesives and biofuel knowledge base and stimulate future developments within the biobased adhesive and biofuel industry. In addition, biomass and bioenergy industries will be the immediate beneficiaries of the advanced technologies for biofuel production. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The overall goal of this project is to develop enabling technologies that utilize agriculture feedstocks and by-products for biobased materials and bioenergy, and to generate new knowledge that can be useful reference to both academia and industries in the field. In the past year, we improved wet adhesion of plant protein (soybean, cottonseeds) based adhesives modified with depolymerized lignins. We also conducted research on cellulosic biofuels with focus on biomass pretreatment and improved biomass fermentation yield and efficiency.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Pradyawong, S., J. Li, Z. He, X.S. Sun, D. Wang, H.N. Cheng, K. T. Klasson. 2018. Blending cottonseed meal products with different protein contents for cost-effective wood adhesion performances. Industrial Crops and Products 126(2018) 31-37. https://doi.org/10.1016/j.indcrop.2018.09.052
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Pradyawong, S., G. Qi, X.S. Sun, D. Wang. 2019. Laccase/TEMPO-Modified Lignin Improved Soy-Protein-Based Adhesives: Adhesion Performance and Properties. International Journal of Adhesion and Adhesives 91 (2019) 116-122. (https://doi.org/10.1016/j.ijadhadh.2019.03.005
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Li, J., S. Pradyawong, Z. He, S.X. Sun, D. Wang, H. Cheng, J. Zhong. 2019. Assessment and application of phosphorus/calcium-cottonseed protein adhesive for plywood production. Journal of Cleaner Production 229:454-462 (https://doi.org/10.1016/j.jclepro.2019.05.038)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Li, X., Shi, J., Das, L., Tharayil, N., Zheng, Y., 2018. Novel platform for bioupgrading of lignin to valuable nutraceuticals and pharmaceuticals. 2018 ASABE Proceeding, Detroit, MI, USA.
  • Type: Other Status: Other Year Published: 2019 Citation: Zheng, Y., 2019. A novel platform for lignin upgrading to high-value products with marine protist. USA 62/878,873.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: J. Li, D. Wang. 2018. Rapid and simultaneous determination of furfural and 5-hydroxymethyfurfural in biomass hydrolysis by high-performance liquid chromatography. ASABE Annual Meeting, 7/29 to 8/1, 2018, Detroit, MI., US. Paper No. 1800178.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Y. Yang, M. Zhang, J. Li, D. Wang. 2019. Effects of particle size on biomass pretreatment for biofuel production. Proceeding of the ASME 2019 14th International Manufacturing Science and Engineering Conference, 6/10-14, 2019, Erie, PA.