Progress 12/01/18 to 11/30/22
Outputs Target Audience:The target audiences reached by our efforts during this reporting period included industry professionals, researchers in the engineering and animal sciences fields, and the general public. Changes/Problems:The produced rumen robot prototype was not capable of executing sensing tasks independently. Therefore, we used a simulated robot technique to generate the sensing data needed to complete the work proposed in the project. Now that we have a working version of the robot prototype and have identified concise sensing capacity needed (i.e., we can accomplish our target sensing with pH and temperature alone), we will explore strategies to leverage the pH sensing in future robotics work. What opportunities for training and professional development has the project provided?Graduate students assigned to the project have gained invaluable research skills in the planning and execution of interdisciplinary research as well as training in the appropriate methods (laboratory and statistical) necessary to generate and interpret the study results. Several undergraduate students were able to gain experience with research animals and laboratory methods through their roles as research assistants. Regarding professional development, graduate students benefited from a number of opportunities to present their study results to the scientific community at regional, national, and international animal science meetings. How have the results been disseminated to communities of interest?Results have been disseminated to producers through industry-oriented presentations at state Extension field days. Dissemination to the scientific connumity has taken place through presentation of research findings are scientific meetings such as the annual meeting of the American Society of Animal Science and the annual meeting of the American Dairy Science Association. Dissemination to the general public has occured through showcase presentations to Virginia Tech alumni during local outreach events. What do you plan to do during the next reporting period to accomplish the goals?Although this is our final report on this project, I would point out we have a number of papers on the in vivo testing that are yet to be published. We will continue to work toward publication of those papers over the next several months.
Impacts What was accomplished under these goals?
Design and build a cow rumen ROV, Several prototype robots were constructed and the power requirements to achieve sufficient thrust for navigation within the rumen environment were tested. A bio-inspired fish design was selected for subsequent testing. Evaluate the ROV as a remote sensing tool in a simulated rumen environment andin vivo, We constructed a synthetic rumen environment for testing the robotic protoype which can flexibly be adjusted to simulate the viscosity of the rumen fulid, the layers of fluid within the rumen, the chemical composition of that fulid or the motion of the fluid. These different elements of the environment can also be tested together. We tested the ability of the ROV to locomote within that environment, and explored how environmental parameters influenced the capacity of the ROV to travel, as measured by the rate of travel and the precision intraveling to a targeted point. Demonstrate the ability of the ROV to collect real-time rumen data over long time scales, Real-time sensing and long-term evaluation of the rumen environment was explored through sensing of pH, temperature, and dissolved CO2 concentrations. These data were collected for multiple fermentation cycles in animals exposed to diets verying in fermentability characteristics. These data suggest strong capacity to leverage pH to predict key fermentation parameters like rumen VFA profiles and levels throughout the fermentation. This finding will be further explored in future work. Demonstrate the ability of the ROV to collect rumen tissue and fluid samples over short time scales, The prototype ROV was not able to collect rumen tissue and fluid samples, however, the rumen fluid sensing successes deemed collection of these samples unnecessary. Synthesize data to create maps and visualizations of a single cow rumen ecosystem, We developed Bayesian Learning Networks of the rumen environment to explore causal inference among rumen fermentation indicators and mechanisms. These models show tremendous predictive capacity based on average fermentation data, and notable strength when real-time data are used for feeding. Agglomerate data from multiple cows to begin enabling scaling and generalization. Data from multiple cows outfitted with pH, temperature and dissolved CO2 sensing equipment were agglomerated and compared. Although different animals had different VFA profiles and extents of fermentation, the relationships identified between sensed parameters and VFA profiles were consistent across cows and diets, suggesting that these sensed metrics have high generalizability as a strategy for future study of the rumen ecosystem.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
S. Sujani* and R.R White, 2022. Effect of diet, rumen fermentation parameters and the combination on milk production/ feed efficiency. Proceedings of the Annual Meeting of the American Animal Science Association, USA.
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
A. Knoepfel, N. Liu, Y. Hou, S. Sujani*, B.R. Dos Reis*, R. White, K. Wang, B. Poudel, S. Gupta, and S. Priya. 2022. Development of tetrapod zinc oxide-based UV sensor for precision livestock farming and productivity. Biosensors. https://doi.org/10.3390/bios12100837
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
U. Kaur, R. Sriramdas, X. Li, X. Ma, A. Datta, B.R. dos Reis*, S. Sen, K. Daniels, R. White, R.M. Voyles, and S. Priya. 2022. Indwelling robots for ruminant health monitoring: a review of elements. Smart Agricultural Technology. 3:100109 https://doi.org/10.1016/j.atech.2022.100109
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
V. Souza , D.L. Liebe*, T.P. Price*, M.D. Ellet, T.C. Davis*, C.B. Gleason*, K.M. Daniels, and R.R. White. 2022. Algorithm development for individualized precision feeding of supplemental top dresses to influence feed efficiency of dairy cattle. J. Dairy Sci. https://doi.org/10.3168/jds.2021-20841
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Han, C.S., U. Kaur, B. Huiwen, B. Roqueto dos Reis*, R.R. White, R. Nawrocki, R. Voyles, M.G. Kang, and S. Priya. 2022. Invited Review: Sensor technologies for real-time monitoring of the rumen environment. J. Dairy Sci. https://doi.org/10.3168/jds.2021-20576
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Price, T.C.*, V.C de Souza , D.M. Liebe*, M.D. Elett, T.C. Davis*, C.B. Gleason*, K.M. Daniels, and R.R. White. 2021. Short-term adaptation of dairy cattle production parameters to individualized changes in dietary top dress. Animals. 11(12):3518. https://doi.org/10.3390/ani11123518
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
B.R. dos Reis*, S. Sujani*, and R. R. White. Additive Bayesian network for systems-oriented meta-analysis. 10th Workshop on Modelling Nutrient Digestion and Utilization in Farm Animals, 2022. Sardinia, Italy
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
B. R dos Reis*, C. B. Gleason**, and R. R. White. Bayesian network probabilistic modeling for understanding rumen dynamics. EAAP International Symposium on Energy and Protein Metabolism and Nutrition, 2022. Spain.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
S. Sujani*, C. B. Gleason**, B. R. dos Reis*, and R. R. White. Time-series assessment of rumen fermentation from meal-fed sheep. 10th Workshop on Modelling Nutrient Digestion and Utilization in Farm Animals, 2022. Sardinia, Italy.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
S. Sujani*, C.B Gleason**, B.R dos Reis* and R.R. White, 2022. Changes in rumen volatile fatty acid production in response to the varying degradability of fibre and protein. 7th EAAP International Conference, Spain.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
S. Sujani* and R.R White, 2022. Composition of milk as explained by rumen fermentation parameters, diet composition and combination: a meta-analysis. Proceedings of the Annual Meeting of the American Animal Science Association, USA.
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Progress 12/01/20 to 11/30/21
Outputs Target Audience:The target audiences reached by our efforts during this reporting period included industry professionals, researchers in the engineering and animal sciences fields, and the general public. Changes/Problems:The COVID-19 pandemic delayed the protoyping of the robot at Penn State. As such, we had to purchase commercial data loggers and sensors to begin collecting preliminary data we originally had anticipated the robot to collect. In the coming months, we expect to leverage that data into publications and into more targeted and informed use of the robot in initiial in vivo testing. What opportunities for training and professional development has the project provided?Graduate students assigned to the project have gained invaluable research skills in the planning and execution of interdisciplinary research as well as training in the appropriate methods (laboratory and statistical) necessary to generate and interpret the study results. Several undergraduate students were able to gain experience with research animals and laboratory methods through their roles as research assistants. Regarding professional development, graduate students benefited from a number of opportunities to present their study results to the scientific community at regional, national, and international animal science meetings. How have the results been disseminated to communities of interest?Results have been disseminated to producers through industry-oriented presentations at state Extension field days. Dissemination to the scientific connumity has taken place through presentation of research findings are scientific meetings such as the annual meeting of the American Society of Animal Science and the annual meeting of the American Dairy Science Association. Dissemination to the general public has occured through showcase presentations to Virginia Tech alumni during local outreach events. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period we plan to progress to in vivo testing of the ROV prototype.
Impacts What was accomplished under these goals?
The following was accomplished under these goals: Design and build a cow rumen ROV, A prototype robot has been constructed and the power requirements to achieve sufficient thrust for navigation within the rumen environment were tested. A site test of the prototype in a simulated rumen environment is underway. Evaluate the ROV as a remote sensing tool in a simulated rumen environment andin vivo, We constructed a synthetic rumen environment for testing the robotic protoype which can flexibly be adjusted to simulate the viscosity of the rumen fulid, the layers of fluid within the rumen, the chemical composition of that fulid or the motion of the fluid. These different elements of the environment can also be tested together. Demonstrate the ability of the ROV to collect real-time rumen data over long time scales, We have a trial planed for Spring 2022 to conduct this objective, pending the arrival of the most recent robotic prototype. Due to delays in the prototyping process, we purchased a commercially available sensor system in 2021 and began testing the spatial heterogeneity of the rumen environment with that system. These results are currently being analyzed and publications are expected in 2022. Demonstrate the ability of the ROV to collect rumen tissue and fluid samples over short time scales, We have a trial planed for Spring 2022 to conduct this objective, pending the arrival of the most recent robotic prototype.Due to delays in the prototyping process, we purchased a commercially available sensor system in 2021 and began testing the temporal heterogeneity of the rumen environment with that system. These results are currently being analyzed and publications are expected in 2022. Synthesize data to create maps and visualizations of a single cow rumen ecosystem, In light of delays in the robot construction and ptotyping process, we have collected literature data to begin modeling the rumen environment in more detail before we advance to models based on rumen sensed metrics. We are currently evaluating and comparing models and publications are expected in 2022. Agglomerate data from multiple cows to begin enabling scaling and generalization. We expect to conduct this wokr after experimental testing in summer of 2022.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Han, C.S., U. Kaur, B. Huiwen, B. Roqueto dos Reis, R.R. White, R. Nawrocki, R. Voyles, and S. Priya. 2022. Invited Review: Sensor technologies for real-time monitoring of the rumen environment. J. Dairy Sci.
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Gleason, C.B.*, L.M. Beckett*, B. Roqueto dos Reis*, and R.R. White. 2021. Evaluating the relationship between in vitro and in situ starch degradation rates. Animal Feed Science and Technology. 115175.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2021
Citation:
Sujani, S.*, Wenner, B., Firkins, J. L., & White, R. R. (2020). A network analysis of continuous culture fermentation data.. In JOURNAL OF DAIRY SCIENCE Vol. 103 (pp. 160). ELECTR NETWORK: ELSEVIER SCIENCE INC. Retrieved from http://gateway.webofknowledge.com/
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2021
Citation:
dos Reis, B. R.*, & White, R. R. (2020). An integrated sensor network for monitoring pastured cattle health and location.. In JOURNAL OF DAIRY SCIENCE Vol. 103 (pp. 237-238). ELECTR NETWORK: ELSEVIER SCIENCE INC. Retrieved from http://gateway.webofknowledge.com/
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Progress 12/01/19 to 11/30/20
Outputs Target Audience:The target audiences reached by our efforts during this reporting period included industry professionals, researchers in the engineering and animal sciences fields, and the general public. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Graduate students assigned to the project have gained invaluable research skills in the planning and execution of interdisciplinary researchas well as training in the appropriate methods (laboratory and statistical) necessary to generate and interpret the study results. Several undergraduate students were able to gain experience with research animals and laboratory methods through their roles as research assistants. Regarding professional development, graduate students benefited from a number of opportunities to present their study results to the scientific community at regional, national, and international animal science meetings. How have the results been disseminated to communities of interest?Results have been disseminated to producers through industry-oriented presentations at state Extension field days. Dissemination to the scientific connumity has taken place through presentation of research findings are scientific meetings such as the annual meeting of the American Society of Animal Science and the annual meeting of the American Dairy Science Association. Dissemination to the general public has occured through showcase presentations to Virginia Tech alumni during football weekends and at local alumni events. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period we plan to progress to in vivo testing of the ROV prototype.
Impacts What was accomplished under these goals?
Design and build a cow rumen ROV, A working model of the cow rumen ROV was designed and fabricated. A number of different locomotion strategies were developed, of which a longitudinal wave motion approach was identified as the most ideal and was implemented into the designed and fabricated ROV. A novel pH sensor was also fabricated which can be added into the cow rumen ROV to monitor ruminal pH. The fabricationand testing of the ROV was hindered significantly by the COVID-19 pandemic because the engineering laboratory facilities at Penn State were closed for several months. Evaluate the ROV as a remote sensing tool in a simulated rumen environment andin vivo, In vitro assessments of the ROV were conducted in water, geletin and simulated rumen fluid. These tests were conducted to evaluate necessary improvements in the longitudinal wave motion mechanism likely to be necessary prior to successful in vivo testing. In vitro testing of the novel pH sensor was also conducted and the sensor demonstraated excellent repeatability and linearity. In vivo testing is planned for the 2021 calendar year. Demonstrate the ability of the ROV to collect real-time rumen data over long time scales, Due to delays in the robot fabircation caused by COVID-19 we have not progressed to in vivo testing yet. This is planned for 2021. Demonstrate the ability of the ROV to collect rumen tissue and fluid samples over short time scales, Due to delays in the robot fabircation caused by COVID-19 we have not progressed to in vivo testing yet. This is planned for 2021. Synthesize data to create maps and visualizations of a single cow rumen ecosystem, Due to delays in the robot fabircation caused by COVID-19 we have not progressed to in vivo testing yet. This is planned for 2021. Agglomerate data from multiple cows to begin enabling scaling and generalization. Due to delays in the robot fabircation caused by COVID-19 we have not progressed to in vivo testing yet. This is planned for 2021.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
dos Reis, B. R.*, Fuka, D. R., Easton, Z. M., & White, R. R. (2020). An open-source research tool to study triaxial inertial sensors for monitoring selected behaviors in sheep. Translational Animal Science, 4(4). doi:10.1093/tas/txaa188
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Gleason, C. B.*, White, R. R., & Schramm, H. H. (2020). Influence of two rumen cannulation techniques on postoperative recovery in sheep. VETERINARY SURGERY, 11 pages. doi:10.1111/vsu.13547
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
dos Reis, B.R.*, Z. Easton, D. Fuka, and R.R. White. 2021. A LoRa sensor network for monitoring pastured livestock location and activity. Translational Animal Science. DOI: https://doi.org/10.1093/tas/txab010
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Souza, V. and R.R. White. 2021. Variation in urea kinetics associated with ruminant species, dietary characteristics, and ruminal fermentation: A meta-analysis. Journal of Dairy Science. DOI: https://doi.org/10.3168/jds.2020-19447
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Sujani, S.*, Wenner, B., Firkins, J. L., & White, R. R. (2020). A network analysis of continuous culture fermentation data.. In JOURNAL OF DAIRY SCIENCE Vol. 103 (pp. 160). ELECTR NETWORK: ELSEVIER SCIENCE INC. Retrieved from http://gateway.webofknowledge.com/
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
dos Reis, B. R.*, & White, R. R. (2020). An integrated sensor network for monitoring pastured cattle health and location.. In JOURNAL OF DAIRY SCIENCE Vol. 103 (pp. 237-238). ELECTR NETWORK: ELSEVIER SCIENCE INC. Retrieved from http://gateway.webofknowledge.com/
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
dos Reis, B. R.*, Poudel, B., Priya, S., & White, R. R. (2020). Thermoelectric energy harvesting for wearable precision agriculture technologies.. In JOURNAL OF DAIRY SCIENCE Vol. 103 (pp. 238). ELECTR NETWORK: ELSEVIER SCIENCE INC. Retrieved from http://gateway.webofknowledge.com/
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Progress 12/01/18 to 11/30/19
Outputs Target Audience:The target audience during this reporting period was fellow scientists. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project has provided the graduate students the opportunity to learn, collaborate, and innovate outside their disciplines. They interact as a student group weekly and with the broader faculty group on a monthly basis. Students have also had the opportunity to attend professional society conferences to present research. How have the results been disseminated to communities of interest?Results have been disseminated to the communities of interest through presentations at profesional society conferences. What do you plan to do during the next reporting period to accomplish the goals?We plan to finalize the prototype ROV for in vivo testing. We anticipate needing another round of design refinement after the in vivo testing, which we hope to also accomplish next year so that the remaining project years can be used for data collection.
Impacts What was accomplished under these goals?
Design and build a cow rumen ROV, Several prototype romen ROV have been constructed Different locomotion strategies have been tested A downsized piezoelectric motor is being developed to facilitate downscaling The prototype is being updated with more thrust (per activities under objective 2) and will be outfitted with sensors for in vivo testing in 2020. Evaluate the ROV as a remote sensing tool in a simulated rumen environment andin vivo, The locomation activity of the test ROV has been evaluated in a gelatin-based medium. The test identified the longtiudinal locomotion activity had insufficient thrust to effectively move through the more dense medium. Pending updates to the robot, in vivo testing is expected for 2020. Demonstrate the ability of the ROV to collect real-time rumen data over long time scales, Anticipated 2021 Demonstrate the ability of the ROV to collect rumen tissue and fluid samples over short time scales, Anticipated 2021 Synthesize data to create maps and visualizations of a single cow rumen ecosystem, Anticipated 2022 Agglomerate data from multiple cows to begin enabling scaling and generalization. Anticipated 2022
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2020
Citation:
Roqueto dos Reis, B., and R.R. White. 2020. Thermoelectric Energy Harvesting For Wearable Precision Agriculture Technologies. Annual Meeting of the American Dairy Science Association.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2020
Citation:
B. R. dos Reis, B. Poudel, S. Priya, and R. R. White. 2020. An Integrated Sensor Network for Monitoring Pastured Cattle Health and Location. Annual Meeting of the American Dairy Science Association.
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