Source: UNIV OF HAWAII submitted to NRP
THE SCIENCE AND ENGINEERING FOR A BIOBASED INDUSTRY AND ECONOMY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1017992
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
S-OLD 1075
Project Start Date
Oct 18, 2018
Project End Date
Sep 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF HAWAII
3190 MAILE WAY
HONOLULU,HI 96822
Performing Department
Molecular Biosciences & Bioengineering
Non Technical Summary
The research focus is to convert various low-value feedstocks including organic wastes (water) into bioenergy and biobased production through various microbial mediated processes with concomitant remediation of organic wastes. The research projects include anaerobic digestion of biomass with and without micro-aeration, biochar application in hydrogen sulfide removal, organic wastes to animal feed via insect biorefinery and fungal protein production.
Animal Health Component
35%
Research Effort Categories
Basic
60%
Applied
35%
Developmental
5%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
51174102020100%
Goals / Objectives
Develop deployable biomass feedstock and supply knowledge, processes and logistics systems that economically deliver timely and sufficient quantities of biomass with predictable specifications to meet efficient handling, storage and conversion process requirements Research and develop technically feasible, economically viable and environmentally sustainable technologies to convert biomass resources into chemicals, energy, materials in a biorefinery methodology including developing co-products to enable greater commercialization potential. Perform system analysis to support and inform development of sustainable multiple product streams (chemicals, energy, and materials) and use the insights from the systems analysis to guide research and policy decisions
Project Methods
Quantify and characterize biological feedstocks Chemical composition of energy crops is a key factor affecting efficiency of biofuel and high value co-product generation in biorefineries, and ultimately the economic feasibility of biofuel industries. The composition of energy crops significantly affects both digestibility and overall methane yield during anaerobic digestion. Thus, we will characterize the biomass composition using ANKOM method and its subsequent effects on anaerobic digestibility for CH4 production via biomethane potential (BMP)..Objective B. Improve biofuel production processes.Develop pretreatment methods for biological conversion processesFor the dilute acid pretreatment of Napier grass (Pennisetum purpureum), the factors of Acid Concentration, Time, Temperature and Solid-to-Liquid Ratio will be examined to see what effect and interactive effects these variables had on sugar release. A split plot experimental design will be used to look at 2 levels of Time and Temperature (main plot) and 3 levels of Acid Concentration and Solid to Liquid Ratio (split plot), each combination will be run in duplicate. A predicting model will be generated using stepwise regression. Pretreatment factors of: Time, Temperature, Time X Temperature interaction, Solid to Liquid Ratio, Temperature X Acid Concentration interaction and Temperature X Solid to Liquid Ratio interaction will the most significant on predicting sugar release. Therefore all factors are important, however due to the interactive effects the value of one factor dictates the value of the other factor it interacts with.Develop conversion processes A series of batch experiments will be conducted to investigate the effect of micro-oxygenation on VFAs production from lignocellulosic biomass under mesophilic conditions. Napier grass will be used as the substrate and oxygen was injected into the mixture of biomass and inoculum at dosages (mlO2/gVSadded) of 0 (control), 15, and 30 just before the start of the experiment.As part of AD project, microbial community analysis will be conducted. Three semi-continuous anaerobic bioreactors (R1, R2, and R3 inoculated with rumen content, mixture of both inocula and conventional AD inoculum, respectively) will be operated for the co-digestion of Napier grass and cow manure. The mixed inocula could enhance the biodegradation of Napier grass due to the synergetic action of mixed microbial population. However, the start-up period should be closely monitoredand the use of a co-substrate with high buffering capacity is recommended for the efficient digestion biomassusing rumen contents.Characterize new feedstocksThe University of Hawaii group is workingon using food wastes and other agri-residues for growing black soldier flies (BSF) for biodiesel production. The team has been conducting several field trials in Hawaii to optimize BSF yield under various environmental conditions.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:PD's team has published related papers and books on the themes of the multi-state research with much broader audiences - researchers, graduate and undergraduatestudents, business entities and others interested in this very subject. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A graduate student is currently being trained on various aspects of experimental design, laboratory analysis and bioreactor design for algal biomass production. How have the results been disseminated to communities of interest?Through journal publications as listed under the Products section. What do you plan to do during the next reporting period to accomplish the goals?Optimize algal biomass production through nano-bubble technology. The PD has just started the project due to COVID 19.

Impacts
What was accomplished under these goals? PD has just started the project related to task 2. The progress has been slow due to pandemic.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Oliveira, F.R., Surendra, K.C., Jaisi, D.P., Lu, H., and Khanal, S.K. 2020. Alleviating sulfide toxicity using biochar during anaerobic treatment of sulfate-laden wastewater with simultaneous recovery of sulfur-rich biochar as soil macro-nutrient. Bioresource Technology. 301:122711
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Oginni, O., Yakaboylu, G.A., Singh, K., Sabolsky, E.M., Unal-Tosun, G., Jaisi, D., Khanal, S.K., and Shah, A. 2020. Phosphorus adsorption behaviors of MgO modified biochars derived from waste woody biomass resources. Journal of Environmental Chemical Engineering. 8(2):103723.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Wells, J.M., Drielak, E., Surendra, K.C., and Khanal, S.K. 2020. Hot water pretreatment of lignocellulosic biomass: Modeling the effects of temperature, enzyme and biomass loadings on sugar yield. Bioresource Technology. 300:122593.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Surendra, K.C., Tomberlin, J.K., van Huis A., Cammack, J.A., Heckmann. L-H.L., and Khanal, S.K. 2020. Rethinking organic wastes bioconversion: Evaluating the potential of the black soldier fly (Hermetia illucens L.) (Diptera: Stratiomyidae) (BSF). Waste Management. 117: 58-80


Progress 10/18/18 to 09/30/19

Outputs
Target Audience:Based on our on-going efforts on black soldier fly (BSF) research, we have been able to establish partnership with Hawaii Department of Agriculture (HDOA) to conduct scale-up studies for producing large amount of BSF larvae for aquatic feed production. We have also started a new project on nano-bubble technology in close partnership with HDOA. Changes/Problems:We are exploring the use of nano-bubble technology. We recently initiated this project and received funding from the college. What opportunities for training and professional development has the project provided?The project provided research opportunity for one graduate and one undergraduate students, and one post-doc. They were involved in Laboratory research, data analysis, journal paper preparation. They are continuing on the projects and learning new research skills to make a better contribution to the society. How have the results been disseminated to communities of interest?1. Oliveira, F. and Khanal, S.K*. Alleviating sulfide toxicity using biochar during anaerobic treatment of high-sulfate wastewater with sulfur recovery. 16th World Congress on Anaerobic Digestion, June 23-27, 2019, Delft, The Netherlands (Oral). 2. Invited speaker, Sun Grant Center Western Regional Center, Oregon State University, Corvallis, OR (Aug 21, 2019). "Bioenergy and biobased products from biowastes/agri-residues." 3. Invited speaker, Special seminar at Shandong University, Qingdao (July 9, 2019). "Recovery of resources from wastes (water)" 4. Invited speaker, Special seminar at Hong Kong University of Science and Technology (Jun 12, 2019). "Recovery of resources from wastes (water): some perspectives." 5. Invited speaker, International Conference on Sustainable Solid Waste Treatments and Managements, Yangling, China (May 6-9, 2019). "Organic wastes bioconversion via insect farming: opportunities and challenges." 6. Invited speaker, Special seminar at Prince of Songkla University, Songkhla, Thailand (Apr 19, 2019). "Waste-to-resources: Some perspectives." What do you plan to do during the next reporting period to accomplish the goals?Here we have 2 research projects that will be carried out: 1. The use of BSF to convert organic wastes into aquaculture feed is an emerging field. However, there is a clear need to apply engineering principles to design and operate efficient and safe BSF technologies. Development of system capable of effectively controlling proper temperature, air, and moisture within the reactors will allow three-dimensional (i.e., volume) use of space rather than utilization of two-dimensional (i.e., area) space that is currently being practiced. The growth reactors with built-in process control devices with provision for self-harvesting of BSF biomass will significantly reduce the amount of space and labor requirement for a waste-to-BSF system. This study will also generate data on microbial communities associated with the bioconversion process and the residual insect compost, and quantify the nutrient value and microbial safety of the BSF biomass and insect compost. In addition, a techno-economic analysis of the system will provide valuable insight in determining the commercial feasibility and applicability of the BSF technology. This study will generate pertinent scientific data for BSF process design and operation which could garner greater interest for the deployment of a relatively simple and robust solution to growing aquaculture feed demands and organic waste disposal problems especially in island environments. If successful, aquaculture feed from organic wastes via BSF larval lifecycle can displace a significant part of imported aquaculture feed with minimal land occupation for states like Hawaii and emerging Pacific Island economies which depend on imports and where land is at a premium and biowaste is in abundance. 2. Nanobubbles are an emerging technology in which ultrafine air or oxygen bubbles (100-200 nm) are generated to enhance oxygen mass transfer in the aqueous phase to improve the efficacy of biological systems. Many biological processes are mediated by microbes in which the availability of sufficient DO is critically important. These biological processes include removal of diverse contaminants such as organic matters, nitrogen, phosphorus, and micropollutants among others by aerobic microbes, and recovery of valuable resources such as fungal protein, enzymes, bioplastics, nitrate, and algal cells etc. To the best of our knowledge, in-depth studies on nanobubble technology and its diverse applications are limited. Few studies showed better plant growth with nanobubbles and with shorter seed germination time, and improved water quality and pathogen deactivation. However, little baseline information is available with direct experimental evidence. We plan to test four types of nanobubble generators for the aforementioned applications to obtain the very first baseline data. Our research builds upon our years of research experience on various aspects of biological systems and oxygen mass transfer

Impacts
What was accomplished under these goals? During 2018-2019, we have completed the AD-biochar project and published one paper on this very concept. The AD-biochar study examined the use of biochar to alleviate sulfide toxicity to methanogens and sulfate-reducing bacteria (SRB) during anaerobic treatment of sulfate-rich wastewater with concomitant sulfur recovery. At the sulfate concentration of 6,000 mg SO42-/L, the dissolved sulfide (DS) of 131 mg S/L resulted in total volatile fatty acids concentration of 3,500 mg/L as acetic acid (HAc) and the reactors were on the verge of failure. Biochar removed >98% of H2S(g), 94% of DS and 89% of unionized sulfide (H2Saq). This study showed that biochar could effectively remove H2S from biogas, alleviate sulfide toxicity to MPA and SRB and promote stability of the anaerobic process. We have been able to attractand develop local colony of black soldier flies (BSF). BSF larvae can convert diverse organic wastes into protein and fat that can be processed into animal feed. Based on outcome of the project, we are now conducting new scale-up project with support from HDOA. We have just completed one state-of-the-art review on this very subject.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Phuttaro, C., Sawatdeenarunat, C., Surendra, K.C., Boonsawang, P., Chaiprapat, S., and Khanal, S.K. 2019. Anaerobic digestion of hydrothermally-pretreated lignocellulosic biomass: Influence of pretreatment temperatures, inhibitors and soluble organics on methane yield. Bioresource Technology. 284: 128-138.
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Rajendran, K., Surendra, K.C., Tomberlin, J.K., and Khanal, S.K. 2018. Insect-based biorefinery for bioenergy and biobased products: A critical review. In Waste Biorefinery: Potential and Perspectives. (eds. Ashok Pandey, Thallada Bhaskar, Ventaka Mohan, D.-J. Lee and Samir Kumar Khanal). Elsevier Inc., USA. Pp 657-669.