Source: UNIVERSITY OF ARKANSAS submitted to NRP
DEVELOPMENT AND EVALUATION OF HYBRID RICE CULTIVARS ADAPTED TO THE SOUTHERN U.S.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1017345
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2018
Project End Date
Sep 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
Crop, Soil & Environmental Sciences
Non Technical Summary
The main objective of this program is to develop hybrid rice cultivars adapted to the Southern U.S. which have high grain and milling yields, desirable plant type, resistance to prevalent diseases and typical U.S. long grain quality. Procedures for breeding and identification of hybrids, restorers and maintainers share similarities to those used for inbred line development. Breeding for and identifying both 2- and 3- line male sterile systems is more complex, but basic breeding principles still apply. Once identified, characterization of the type of CMS system or EGMS genes is needed to consistently produce hybrid seed. A large number of test crosses between potential restorers and male-sterile lines will be needed to select the best hybrid cultivar.Allocation of time to seed production will be necessary to determine which combinations produce the best hybrid seed yields. Collaborative research efforts between the University of Arkansas hybrid rice breeding program and other faculty within the University of Arkansas system, as well as other domestic and international institutions will greatly aid our efforts.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20115301081100%
Knowledge Area
201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1530 - Rice;

Field Of Science
1081 - Breeding;
Goals / Objectives
1. Develop and/or obtain breeding lines to develop hybrid parental lines for both two and three lines systems adapted to the Southern U.S. rice growing region.2. Develop elite hybrid cultivars that have high grain and milling yields with typical U.S. long grain desirable plant type, and resistance to prevalent diseases.3. Develop of inbred lines in parallel with hybrid line development.4. Implement and apply new marker technologies and methodologies to aid inthe identification of marker-trait associations to improve selection efficiency and increase productivity.5. Develop an effective and economical method for hybrid seeds production as well as hybrid rice parental lines seed increase.6. Apply large-scale mechanization to improve the efficiency of the hybrid rice breeding program.7. Foster collaborative research agreements between the University of Arkansas hybridrice breeding program and other faculty within the University of Arkansas system, as well as other domestic and international institutions.
Project Methods
A pedigree-based system will largely be used in the development of male-sterile, restorer and maintainer lines. Single seed descent will be used for specific mapping projects, where individual identification needs to be maintained. Single crosses, top-crosses, three-way crosses and backcrosses will be used for improvement of these lines. Determining which cross to use will largely depend on the types of traits, number of traits and underlying genetic mechanism of the traits we are trying to improve. Additionally, the use of Marker-assisted backcrossing (MAS) and genotyping-based sequencing (GS) will be explored. By selecting for a larger number of markers distributed across the genome, we hope to catch minor alleles which have an additive effect on desired traits. Concomitantly, the goal is to increase the frequency of favorable genes for known agronomic, quality and disease resistance traits within our breeding material.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:The target audiance reached by my efforts includes Arkansas's rice farmers and rice breeding researchers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Our main focus in 2021 will be to produce experimental hybrid rice, develop new techniqeus for the hybrid rice seed production and field management.

Impacts
What was accomplished under these goals? Our achievements in 2020 comprised of 1) development of several male sterile lines, including 3 advanced male sterile lines, which are currently being evaluated for seed yield production, 2) development of 13 rice lines to serve as male parents (pollen donors) for hybrid rice production, including 13 new restorer lines (R line) for the "Three-line hybrid rice system" and two lines for "Two line hybrid rice system, 3) production of experimental hybrid rice (F1) seeds resulting from crosses between the UofA male sterile lines and Arkansas elite cultivars for 2021 preliminary test and Arkansas Rice Preliminary Trials (ARPT), 4) evaluation of experimental hybrid lines developed during summer 2019 for yield and quality characteristics 5) Advancing the hybrid line parental lines by growing them at the Puerto Rico rice winter nursery, 6) Evaluation of several experimental hybrid lines through 2020 preliminary/ ARPT studies, 7) detection of genes associated with restorability in an Arkansas restorer line via QTL analysis, and 10) Improvement of techniques used for increased hybrid seed (F1) production.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Singh, N. N., Want, D., Ali, L., Kim, H., Akther, K. M., Harrington, S. E., Kang, J., Shakiba, E., Shi, Y., DeClerck, G., Meadows, B., Govindaraj, V., Ahn, S.-N., Eizenga, G. C., McCouch, S. A Coordinated Suite of Wild-Introgression Lines in Indica and Japonica Elite Backgrounds. Frontiers in Plant Science
  • Type: Other Status: Published Year Published: 2020 Citation: Hemphill, C. C., Esguerra, M. Q., Moldenhauer, K. A.-K., Sha, X., Shakiba, E., Counce, P. A. (2020). Comparing the Effects of Multiple Planting Dates on Rice Grain Yield and Quality
  • Type: Other Status: Published Year Published: 2020 Citation: Shakiba, E., Moldenhauer, K. A.-K., North, D. G., Boyett, V., Rice, A. (2020). Developing a New Environmental Genic Male-Sterile Line
  • Type: Other Status: Published Year Published: 2020 Citation: Sha, X., Moldenhauer, K. A.-K., Shakiba, E., Hardke, J. T., Wamishe, Y. A., Bateman, Bulloch, Wisdom, McCarty, North, D., Boyett, V., Frizzell (2020). Development of Superior Medium-Grain and Long-Grain Rice Varietiesfor Arkansas and the Mid-South
  • Type: Other Status: Published Year Published: 2020 Citation: Moldenhauer, K. A.-K., Sha, X., Shakiba, E., Hardke, J. T., Wamishe, Y. A., M., Northcut, Wisdom, Belmar, Kelsey, Boyett, V., Thompson, V., Frizzell, Bulloch, Castaneda-Gonzalez, North, D., Beaty (2020). Jewel, High Yielding, Short Season Long-Grain Rice Variety
  • Type: Other Status: Published Year Published: 2020 Citation: " Shakiba, E., Moldenhauer, K. A.-K., Sha, X., Counce, P. A., Scott, R. C., Bathke, G., North, D. G., Boyett, V., Thompson, V., Rice, A. D., Azapoglu, O., Esguerra, M. Q. (2020). Progress in Hybrid Rice Breeding Program
  • Type: Other Status: Published Year Published: 2020 Citation: Rice, A. D., Shakiba, E., Moldenhauer, K. A.-K., Pereira, A. B., Shi, A. (2020). Quantitative Trait Loci Mapping of Panicle Architecture and Yield-Related Traits BetweenTwo U.S. Rice Cultivars LaGrue and Lemont
  • Type: Other Status: Published Year Published: 2020 Citation: Hardke, J. T., Frizzell, D. L., Castaneda-Gonzalez, Frizzel, T. D., Hale, Clayton, Moldenhauer, K. A.-K., Sha, X., Shakiba, E., Wamishe, Y. A., Bulloch, Beaty, McCarty, Runsick, Duren, Mann, Clark, Ablao (2020). Arkansas Rice Performance Trials, 2017-2019


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Our target audience were the Arkansas rice farmers, and rice researchers, and visitors and scholars from different academic institutions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Our plant for the next reporting period is to 1) evaluate experimental hybrid rice in ARPT test, 2) evaluate experimental hybrid rice in a preliminary test 3) identify best combinations for hybrid rice production by crossing male sterile lines with elite cultivars and advanced rice lines, 4) develop new hybrid rice parental lines.

Impacts
What was accomplished under these goals? Our main focus in 2018-2019 was to 1) evaluate experimental hybrid rice in ARPT test, 2) evaluate experimental hybrid rice in a preliminary test 3) identify best combinations for hybrid rice production by crossing male sterile lines with elite cultivars and advanced rice lines, 4) develop new hybrid rice parental lines, and 5) finalize developing new set of male sterile (ms) lines, UAS19. Hybrid Rice Development For the first time, three experimental hybrid rice were tested in 2019 ARPT test. Due to limited amount seed availability, the lines were tested in three locations. The results showed that the hybrid rice developed by new male sterile line have good yield potential. We continue to evaluate our hybrid lines in 2020. Our new strategy in hybrid breeding program is to increase seeds from high performance experimental hybrid lines for ARPT trial and other studies by growing their parents in larger plots as well as implementing required field management e.g. application of gibberellic acid. A Preliminary (heterosis) study was performed for evaluating 85 experimental hybrid rice in three locations of Stuttgart (with three replications), and Pine Tree and Kiser (each with two replications) in summer 2019. These lines were evaluated for several phenotypic charctersitics e.g. lodging, shattering, plant height, heading date, uniformity etc. Presently, we are in process of evaluating seed yield performance and eating and milling quality. The superior experimental hybrid lines will be selected and placed in ARPT trial. A total of 500 possible cross combinations between UAS19 and different rice lines assigned as male parents were constructed in 27 bays located in RREC. The female parent (sterile plant) from each combination was carefully harvested and threshed. The hybrid seeds from those combination with highest amount of F1 seed production will be used for 2020 preliminary study. Developing Hybrid Parental Lines We continued developing both male and female (male sterile) parental lines for hybrid rice production. Currently, numbers of populations that include more than 4000 lines from early (F1) to advanced (F7) generations are grown in RREC. Developing New Male Sterile Line Final evaluation on the new set of male sterile (ms) lines, UAS19, including 11 isogenic lines for several agronomic traits such as phenotypic charctersitics, seed set combination, heading date, etc. were performed in summer 2019. The goal was to select the superior lines that 1) possess genes associated with eating quality and non-aroma, 2) contain semi-dwarf gene for plant height, 3) show tolerance to diseases, 4) produce male sterile seeds in winter nursery, 4) able to produce enough F1 (hybrid) seeds. As a result, three lines were selected for future hybrid rice production.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Shakiba. E a, and R.C. Scott. Hybrid Rice Technology: An Overview. Am J Biomed Sci & Res. 2019 - 5(1). AJBSR.MS.ID.000865. DOI: 10.34297/ AJBSR.2019.05.000865
  • Type: Other Status: Published Year Published: 2019 Citation: 1) Shakiba, E.a, K.A.K. Moldenhauer, X. Sha etc. 2019. Hybrid rice program in 2018. In: R. Norman and KAK. Moldenhauer (eds). B.R. Wells Arkansas Rice Research Studies 2018; 659: 93-97 2) Rice, A.D., K.A.K. Moldenhauer, A. Pereira, A. Shi, and E. Shakibab.2019. Identification of quantitative trait loci (QTLs) for Yield Traits in LaGrue rice. In: R. Norman and KAK. Moldenhauer (eds). B.R. Wells Arkansas Rice Research Studies 2018; 659: 74-79. 3) Boyett, V.A. V.I. Thompson, K.A.K. Moldenhauer, E. Shakiba, etc. 2019. benefits of molecular analysis in rice breeding programs In: R. Norman and KAK. Moldenhauer (eds). B.R. Wells Arkansas Rice Research Studies 2018; 659: 49-52 4) Esguerra, M.Q, R. Dhakal, C.C. Hemphill, &, E. Shakiba etc. 2019. Yield and grain quality characterization of diverse rice cultivars for identification of donors for high nighttime temperature (HNT) tolerance. In: R. Norman and KAK. Moldenhauer (eds). B.R. Wells Arkansas Rice Research Studies 2018; 659: 53-60 5) Moldenhauer, K.A.K., X, Sha, E. Shakiba etc. 2019. Breeding and evaluation for improved rice varietiesthe Arkansas rice breeding and development program. In: R. Norman and KAK. Moldenhauer (eds). B.R. Wells Arkansas Rice Research Studies 2018; 659: 61-66 6) Sha, X., K.A.K. Moldenhauer, E. Shakiba etc. 2019. Development of superior medium-grain and long-grain rice varietiesfor Arkansas and the Mid-South. . In: R. Norman and KAK. Moldenhauer (eds). B.R. Wells Arkansas Rice Research Studies 2018; 659: 87-92.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: 1) Shakiba, E. a, C. Oliveira, D. North, M. McGraw&, and Y. Rahmatallah. 16S rRNA Gene-Based Metagenomic analysis of rhizosphere soil microbiome in Arkansas rice fields. Nov.10-13 ASA-CSSA-SSSA international meeting San Antonio TX. 2) North, D., E. Shakiba bc, C. Fei, P. Counce. Characterization and application of Arkansas male sterile lines for hybrid rice production. Nov.10-13 ASA-CSSA-SSSA international meeting San Antonio TX 3) Adam Rice, E. Shakiba bc, K. Moldenhauer, and A. Preiera. QTL mapping of panicle architecture and yield related traits in two u.s. rice cultivars LaGrue and Lemont. . Nov.10-13 ASA-CSSA-SSSA international meeting San Antonio TX 4) Azapoglu, O., V. Srivastava, E. Shakiba bc. Identification of Genetic Sources of Restorability in Arkansas Restorer Rice Plants. Nov.10-13 ASA-CSSA-SSSA international meeting San Antonio TX. 5) North, D., E. Shakiba bc, C. Fei, P. Counce. Characterization and Application of Arkansas Male Sterile Lines for Hybrid Rice Production. Feb. 21-24 RTWG, MS. 6) Azapoglu, O., V. Srivastava, E. Shakiba bc. Identification of Genetic Sources of Restorability in two Arkansas Restorer Rice lines. Feb. 21-24 RTWG, MS.
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Dustin North, Characterization and Application of Arkansas Male Sterile Lines for Hybrid Rice Production. Iowa State University