Source: IOWA STATE UNIVERSITY submitted to NRP
REGIONAL AQUACULTURE CENTER - NORTH CENTRAL REGION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1017322
Grant No.
2018-38500-28887
Cumulative Award Amt.
$1,858,560.00
Proposal No.
2018-05984
Multistate No.
(N/A)
Project Start Date
Sep 1, 2018
Project End Date
Aug 31, 2023
Grant Year
2019
Program Code
[CA]- Aquaculture Centers
Recipient Organization
IOWA STATE UNIVERSITY
2229 Lincoln Way
AMES,IA 50011
Performing Department
Nat Res Ecology & Management
Non Technical Summary
There are a number of limitations that still affect expansion and profitability of aquaculture in the North Central Region which will be addressed in projects funded by the Center. These limitations include, in part, limited extension/education products, knowledge of possible species selection, regulations, economics, marketing and alternative feedstuffs for cultured animals. The purpose of the North Central Regional Aquaculture Center (NCRAC) is to conduct viable research, extension, and demonstration projects which will overcome these limitations. The projects funded by the Center are competitively solicited and reported on annually to NIFA. Expected outcomes and anticipated benefits for each of the Center-funded projects is presented in the annual reports and those reports as well as the project proposals are posted on the Center's web site at www.ncrac.org.
Animal Health Component
80%
Research Effort Categories
Basic
20%
Applied
80%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3073719101010%
3033719108010%
9033799302030%
3013799108115%
9033799310025%
6013799310010%
Goals / Objectives
To support the North Central Regional Aquaculture Center (NCRAC) for aquaculture research, development, and demonstration; for the enhancement of viable and profitable commercial aquaculture production in the United States for the benefit of producers, consumers, and the American economy; and to utilize the Regional Center in a national program of cooperative and collaborative research, extension, and developmental activities among public and private institutions having demonstrated capabilities in support of commercial aquaculture in the United States. Projects funded by NCRAC should lower the cost, increase production or production efficiency, or increase the profitability for those commercially producing aquatic products in the 12-state North Central Region.
Project Methods
The North Central Regional Aquaculture Center (NCRAC) is an administrative center that consists of a Director, a Board of Directors, an Industry Advisory Council, and a Technical Committee that consists of research and extension contacts. The Board of Directors, based on input from the Industry Advisory Council working in conjunction with the Technical Committee, selects priority areas in research, extension, industry development and emerging issues for which projects to be funded by the Center will be developed. Those projects are developed through a competitive-solicitation process, extensively peer reviewed, and ultimately approved by the Center's Board of Directors before being submitted in a Plan of Work to USDA's National Institute of Food and Agriculture (NIFA) for their approval. Upon approval by NIFA, the Center issues subcontracts to the funded participants for all of the projects contained in the Plan of Work. Progress made on projects in the Plan of Work is included in the annual progress report for the Center that is submitted to NIFA. The annual report includes a project summary, description of progress in meeting stated regional goals and objectives for each funded project, work planned for the coming year, outcomes and impacts of the funded projects, and a cumulative listing of all publications, manuscripts, papers presented, and other outputs for all funded projects.

Progress 09/01/18 to 06/02/23

Outputs
Target Audience:The North Central Regional aquaculture community includes both established aquaculturists and those interested in aquaculture as part of their agriculture diversification. In addition, supporting industries, i.e., commercial fish feed producers and manufacturers, and fish wholesalers and retailers, University and agency personnel (research and extension/outreach), and federal/state/local government officials. Changes/Problems:Ongoing pandemic issues have resulted in delays in the 2022 programs which also led to project delays in 2023. Final project reports will be issued in the 2024 annual report. What opportunities for training and professional development has the project provided?In the previously funded projects, there is a combination of undergraduate and graduate students being trained in aquaculture as part of their involvement in project development. Examples of this training include cryopreservation methods for walleye sperm, use of enriched live foods for larval fish, and training students in basic aquaculture. How have the results been disseminated to communities of interest?Activities were communicated among the NCRAC community members during the 2023 NCRAC annual meeting. All projects are on the current NCRAC website for public information. The NCRAC administrative staff have continued to refine the NCRAC website to allow for easier information transfer to all members of the NCRAC community, i.e., fact sheets, videos and annual reports. Also, this website has continued to be updated in March 2022 with current rules and regulations by state and federal agencies as well as time sensitive announcements. What do you plan to do during the next reporting period to accomplish the goals?In addition to the next annual report for the current research projects, identified fact sheets and workshops will be developed by the funded research and extension staff with guidance by the NCRAC administrative unit. It is anticipated that there will be increased use of web-based portals for content delivery to the aquaculture community of information garnered by the new projects as well as those projects that continuing on from previous grants.

Impacts
What was accomplished under these goals? The NCRAC annual meeting was an in-person conference on February 15, 2023. The Industry Advisory Committee (IAC), Technical Committee/Research (TC/R), and Technical Committee/Extension (TC/E) committees met in separate groups and then later all together for discussion. Project presentations were made during the meeting by the lead PI or designee. The NCRAC annual board meeting was held the evening of February 15, 2023. Results from the Listening Session held on February 16, 2023 will be discussed in a future board meeting. Progress on the following projects is below: Addressing critical Aquaculture-marketing-oriented applied research and outreach (Phase 2).The team partnered with nine aquaculture businesses in the North Central Region. Two audio and visual assets videos for the "Local Farmers, Local Fish" and cooking demonstrations were produced. Two fact sheets were also drafted. A needs assessment video was developed in two parts to assess the needs of CES to develop a tool kit and to assess the needs of a toolkit focused on direct marketing. Improving Fish Health in the NCR integrating extension with the development of alternative disease prevention methods.An Extension Aquaculture Veterinarian was hired in the Fall of 2022. This position will provide extension veterinary expertise to aquaculture producers in the NCR, leading to responses to fish health needs. Intensification of first year Largemouth Bass (Micropterus Salmoides) using alternative pond-based production systems.The PIs propose investigating intensified alternative production systems for LMB, which could allow for reduced cannibalism, lowered feed conversation ratios, improved opportunity for sheltering from piscivorous birds, and increased yields. Developing social license for trout aquaculture in the North Central Region.The project team has identified an aquaculture producer in each of the three targeted states (Michigan, Wisconsin, and Ohio) to focus on the Community Advisory Social Panel license effort. The Center for Food Integrity (CFI) was asked to present the project to the annual Aquaculture America Conference on February 28, 2022. Discovery work revealed that the original definition of "community" for a Community Advisory Panel approach does not exist in Michigan and Wisconsin. However, it is clear that social license issue does exist, but on a statewide level. A comprehensive study of processing fish in local facilities for local food systems.A graduate student and an assistant were hired on August 1, 2022. A seafood Hazard Analysis Critical Control Point (HACCP) training was planned to be held in October/November 2022. An in-person training at the University of Illinois is scheduled in Feb/March 2023 with fish producers. The initial project paperwork was delayed so final results are not yet available. Genetically Improved All-Female Walleye (Sander vitreus) for Intensive Aquaculture Production in the Great Lakes Region.The research conducted during 2020 and 2021 directly contributed to the education of undergraduate students enrolled in the OSU Aquaculture course during both spring semesters, as students were trained in fish reproduction, embryology, and larviculture through hands-on learning. The first year of this project also directly contributed to the training of three graduate students, one of which completed her doctorate degree December 2020. Optimization of Practical Feed Formulation to Improve Fish Health and Production of Yellow Perch (Perca flavescens).The preliminary results suggest that 1) different feed processing methods significantly change the utilization of different carbohydrate; 2) a practical diet containing 20% or 25% wheat flour or corn flour depressed growth of yellow perch; 3) dietary lipid at 10% in the commercial feed seems to be sufficient to support a good growth of perch and a high level of 16% dietary lipid in the commercial feed did not promote a better growth. Assessing the Effectiveness of NCRAC Funded Research in Aquaculture Within the North Central Region.The Excel summary table of NCRAC funded research projects from 1994 to 2019 has been developed and is complete. A printed and online survey was conducted and results showed that through NCRAC funded projects and Extension projects, NCR producers had gained awareness of several aquaculture-related issues and had adopted NCRAC project recommended practices that contributed to productivity and profitability of their businesses. Evidence was found that NCRAC projects leveraged other funding sources such as Sea Grant, Animal Plant and Health Inspection Service, private veterinarians, and other agencies to address key industry priorities. Nutritional Programming of Yellow Perch Larvae Using Live Food as a Vehicle.Preliminary results indicated that NP via enriched live food provides benefits to larvae and can effectively improve dietary utilization of plant protein later in life in YP. This allows for the increased use of plant-based feeds in YP rearing which are both lower cost and more environmentally sustainable compared to FM-based diets. Further work is needed to analyze the histological, genetic, and gut microbiome results that will give more information on how NP functions. The following projects have been terminated: Evaluating novel methods for preventing Aeromonas-associated losses in Yellow Perch (Perca flavescens) using laboratory and field-based vaccination trials.The findings of this study have demonstrated that the Aeromonas vaccine developed for salmonids can be used safely in yellow perch. Although further study is needed to determine the level of protection afforded to vaccinated fish, early evidence suggests an unintended but positive side effect is a boost to YP growth and "robustness," as well as a potential tool for reducing bacterial loads in vaccinated fish. Non-Lethal Bird Deterrent Evaluation in the NCR.Six separate evaluations were conducted. Evaluations at Ozark Fisheries facilities were conducted at both their Missouri and Indiana farms. Evaluations were conducted in Spring 2021 at Fountain Bluff, Ozark Fisheries, Missouri, and Harietta Hills, and 2 evaluations were conducted in Fall 2021 at Wholesale Walleye. Overall evaluation of data from all sites, all species and both evaluation approaches (1-day and 1-week) indicated there was not a significant effect (p=0.9215) of the system on time birds spent foraging at aquaculture facilities. Evaluation of individual species of birds at each site was conducted (data not presented due to the limited number of evaluations, high number of bird species, and variation in bird species by site). While statistical analyses were difficult to identify, biological and/or economic significance may be realized by the reduction in birds observed, time spent hunting and potentially reduced losses.

Publications

  • Type: Conference Papers and Presentations Status: Awaiting Publication Year Published: 2021 Citation: Smith M.A. and West W.M. 2021. Welcome and Housekeeping. Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials. presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Smith R. 2021.Vaccinating Yellow Perch Against Aeromonas salmonicida: Recent Data Derived from Trials Involving Vaccinated Yellow Perch, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Smith R. 2021. Vaccinating Yellow Perch Against Aeromonas salmonicida: History of the Aeromonas salmonicida Vaccination Project and how it Came About, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: West W.M., Smith R, Smith M.A. 2021, Shavalier MA, Deng D-F, Keleher W, Loch TP*. Vaccinating Yellow Perch Against Aeromonas salmonicida: An Introduction to the Project & Fish Health Overview, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: West W.M. 2021. Vaccinating Yellow Perch Against Aeromonas salmonicida: A Producers Perspective on Yellow Perch Vaccination, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI


Progress 09/01/21 to 08/31/22

Outputs
Target Audience:The North Central Regional aquaculture community includes both established aquaculturists and those interested in aquaculture as part of their agriculture diversification. In addition, supporting industries, i.e., commercial fish feed producers and manufacturers, fish wholesalers and retailers, University and agency personnel (research and extension/outreach), and federal/state/local government officials. Changes/Problems:Ongoing pandemic issues have resulted in delays in the 2022 programs. Meetings were held virtually and several no cost extensions were required to be issued to subcontractors. What opportunities for training and professional development has the project provided?In the previously funded projects, there is a combination of undergraduate and graduate students being trained in aquaculture as part of their involvement in project development. Examples of this training include cryopreservation methods for walleye sperm, use of enriched live foods for larval fish, and training students in basic aquaculture. How have the results been disseminated to communities of interest?Initial activities were communicated among the NCRAC community members during the 2022 NCRAC annual meeting. All projects are on the current NCRAC website for public information. The NCRAC administrative staff has continued to refine the NCRAC website to allow for easier information transfer to all members of the NCRAC community, i.e., fact sheets, videos, and annual reports. Also, this website has continued to be updated in March 2022 with current rules and regulations by state and federal agencies as well as time-sensitive announcements. What do you plan to do during the next reporting period to accomplish the goals?In addition to the next annual report for the current research projects, identified fact sheets and workshops will be developed by the funded research and extension staff with guidance from the NCRAC administrative unit. It is anticipated that there will be increased use of web-based portals for content delivery to the aquaculture community of information garnered by the new projects as well as those projects that continuing on from previous grants.

Impacts
What was accomplished under these goals? The NCRAC annual meeting was a virtual conference on March 10, 2022, due to travel restrictions related to the Covid-19 pandemic. The Industry Advisory Committee (IAC), Technical Committee/Research (TC/R), and Technical Committee/Extension (TC/E) committees met in separate groups and then later all together for discussion. Project presentations were made during the meeting by the lead PI on each project. The NCRAC annual board meeting was held virtually on March 14, 2022. Outcomes from the NCRAC board included postponing the North Central Regional Aquaculture conference and Listening Session as well the next round of RFPs until March 2023 after a new NCRAC Director is hired. The four new projects that started September 1, 2021,in the initial stage and results are not available at this time, are listed below: Addressing critical Aquaculture-marketing-oriented applied research and outreach (Phase 2). This project will build upon the success of the prior NCRAC-Sea Grant project to conduct innovative aquaculture applied research and outreach throughout the North Central Region. Intensification of first year Largemouth Bass (Micropterus salmoides) using alternative pond-based production systems.The PIs propose investigating intensified alternative production systems for LMB, which could allow for reduced cannibalism, lowered feed conversation ratios, improved opportunity for sheltering from piscivorous birds, and increased yields. Developing social license for trout aquaculture in the North Central Region.The lack of social license for aquaculture is widely cited as a major constraint to growth, as expressed through overly burdensome regulations, delays/denials of permits, and lawsuits by citizens groups. This project has potential to serve as a model beyond the North Central Region (NCR). The project team formed includes NCR aquaculture Extension specialists, economists well known to NCR aquaculture producers, and social license experts. The goal is to develop and test strategies designed to enhance social license for aquaculture in the NCR. A comprehensive study of processing fish in local facilities for local food systems is funded entirely (2 years) from the 2018 grant. This study explores processing fish and other aquaculture products in shared-use commercial kitchen and butcher shop-type facilities in local communities for the growing local food systems. Progress on the following ongoing projects are below: Evaluating novel methods for preventing Aeromonas-associated losses in Yellow Perch (Perca flavescens) using laboratory and field-based vaccination trials.The findings of this study have demonstrated that the Aeromonas vaccine developed for salmonids can be used safely in yellow perch. Although further study is needed to determine the level of protection afforded to vaccinated fish, early evidence suggests an unintended but positive side effect is a boost to YP growth and "robustness," as well as a potential tool for reducing bacterial loads in vaccinated fish. Non-Lethal Bird Deterrent Evaluation in the NCR.This project seeks to evaluate a new non-lethal method of deterring avian predators that relies on disrupting communications among birds, which in turn makes the local area uninhabitable even when an abundance of food is available. Statistical analysis is still underway and publications and videos are in development. Assessing the Effectiveness of NCRAC Funded Research in Aquaculture Within the North Central Region.The Excel summary table of NCRAC funded research projects from 1994 to 2019 has been developed and is complete. There has been no dissemination of results or findings yet from this project. Genetically Improved All-Female Walleye (Sander vitreus) for Intensive Aquaculture Production in the Great Lakes Region.The research conducted during 2020 and 2021 directly contributed to the education of undergraduate students enrolled in the OSU Aquaculture course during both spring semesters, as students were trained in fish reproduction, embryology, and larviculture through hands-on learning. In addition, this project provided four undergraduate interns an opportunity to gain experience in hatchery methods, fish husbandry, and research throughout 2021. The first year of this project also directly contributed to the training of three graduate students, one of which completed her doctorate degree December 2020. Short term outcomes: Increased knowledge of optimized methods to obtain triploid walleye through pressure shocks Increased knowledge of performance (growth, survival) of mixed sex triploid walleyes incomparison to mixed sex diploid walleyes in indoor culture Increased knowledge of methods to obtain gynogenetic walleye through use of irradiated yellowperch or walleye sperm Increased knowledge and optimization of the production and performance from early life stagethrough adulthood of sex reversed gynogenetic walleye Increased knowledge of the transition from live to formulated diets and the treatment of walleyewith MT via feeding method Medium term outcomes: Delivery of technology developed thus far to the scientific community and industryprofessionals (WAS, Hawaii 2020) Undergraduate and graduate students gaining knowledge and understanding of this technologythrough participation in OSU and UWM courses and internships. Nutritional Programming of Yellow Perch Larvae Using Live Food as a Vehicle.Preliminary results indicated that NP via enriched live food provides benefits to larvae and can effectively improve dietary utilization of plant protein later in life in YP. This allows for the increased use of plant-based feeds in YP rearing which are both lower cost and more environmentally sustainable compared to FM-based diets. Further work is needed to analyze the histological, genetic, and gut microbiome results that will give more information on how NP functions. Optimization of Practical Feed Formulation to Improve Fish Health and Production of Yellow Perch (Percaflavescens).Similar to other funded projects, progress on this project was delayed to ongoing pandemic issues. The preliminary results suggest that 1) different feed processing methods significantly change theutilization of different carbohydrate; 2) a practical diet containing 20% or 25% wheat flour or cornflour depressed growth of yellow perch; 3) dietary lipid at 10% in the commercial feed seems to besufficient to support a good growth of perch and a high level of 16% dietary lipid in the commercialfeed did not promote a better growth. The preliminary findings will provide critical information todefine a practical feed formulation for the fish in the future study.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Keleher W. 2021. Vaccinating Yellow Perch Against Aeromonas salmonicida: The Use of Bacterins and Vaccines in Aquaculture, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Smith M.A. and West W.M. 2021. Welcome and Housekeeping. Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: West W.M., Smith R, Smith M.A. 2021, Shavalier MA, Deng D-F, Keleher W, Loch TP*. Vaccinating Yellow Perch Against Aeromonas salmonicida: An Introduction to the Project & Fish Health Overview, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: West W.M. 2021. Vaccinating Yellow Perch Against Aeromonas salmonicida: A Producers Perspective on Yellow Perch Vaccination, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Smith R. 2021. Vaccinating Yellow Perch Against Aeromonas salmonicida: Recent Data Derived from Trials Involving Vaccinated Yellow Perch, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Smith R. 2021. Vaccinating Yellow Perch Against Aeromonas salmonicida: History of the Aeromonas salmonicida Vaccination Project and how it Came About, presented during the USDA-NIFA NCRAC funded Workshop and Farm Tour entitled Vaccinating Yellow Perch Against Aeromonas salmonicida: Lab, Farm, and Field Trials, Sept. 18, 2021, Black Creek, WI.


Progress 09/01/20 to 08/31/21

Outputs
Target Audience:The North Central Regional aquaculture community includes both established aquaculturists and those interested in aquaculture as part of their agriculture diversification. In addition, supporting industries, i.e., commercial fish feed producers and manufacturers, and fish wholesalers and retailers, University and agency personnel (research and extension/outreach), andfederal/state/local government officials. Changes/Problems:The most significant issues are related to Covid-19 restrictions, e.g., travel restrictions to collect research specimens and on-campus limits on research activities. What opportunities for training and professional development has the project provided?In the previously funded projects, there is a combination of undergraduate and graduate students being trained in aquaculture as part of their involvement in project development. Examples of this training include cryopreservation methods for walleye sperm, use of enriched live foods for larval fish, and training students in basic aquaculture. How have the results been disseminated to communities of interest?Initial activities were communicated among the NCRAC communitymembers during the 2021NCRAC annual meeting. All projects are on the current NCRAC web site for public information. TheNCRAC administrative staff have continued to refine the NCRAC web site to allow for easier information transfer to allmembers of the NCRAC community, i.e., fact sheets, videos and annual reports. Also, this web site has continued to beupdated in March 2021with current rules and regulations by state and federal agencies as well as time senstive announcements. What do you plan to do during the next reporting period to accomplish the goals?In addition to the next annual report for the current research projects, identified fact sheets andworkshops will be developed by the funded research and extension staff with guidance by the NCRAC adminstrative unit. It isanticipated that there will be increased use of web-based portals for content delivery to the aquaculture community ofinformation garnered by the new projects as well as those projects that continuing on from previous grants.

Impacts
What was accomplished under these goals? The NCRAC annual meeting was a virtual conference on March 18 and19, 2021 due to travel restrictions related to Covid-19 pandemic. The Industry Advisory Committee (IAC), Technical Committee/Research (TC/R), and Technical Committee/Extension (TC/E) committees met in separate groups and then later all together to discuss the 15 full proposals developed for the 2021 NCRAC RFP on March 18, 2021.The RFP2021 was developed with guidance to PIs to use this information from the 2020 NCRAC Listening Session (https://www.ncrac.org/ncrac-2020-conference-listeningsessions-summary) in developing their proposals. On March 19, the NCRAC Board used outcomes from the previous data as well as external reviews to select eight projects to be funded using a combination of funds from the 2018 and 2020 grants. All new proejcts will start September 1, 2021. The firstyear of four projects will be funded from the 2018grant are: Addressing critical Aquaculture-marketing-oriented applied research and outreach (Phase 2). This project will build upon the success of the prior NCRAC-Sea Grant project to conduct innovative aquaculture applied research and outreach throughout the North Central Region. Intensification of first year Largemouth Bass (Micropterus salmoides) using alternative pond-based production systems. The PIs propose investigating intensified alternative production systems for LMB, which could allow for reduced cannibalism, lowered feed conversation ratios, improved opportunity for sheltering from piscivorous birds, and increased yields. Developing social license for trout aquaculture in the North Central Region. The lack of social license for aquaculture is widely cited as a major constraint to growth, as expressed through overly burdensome regulations, delays/denials of permits, and lawsuits by citizens groups.. This project has potential to serve as a model beyond the North Central Region (NCR). The project team formed includes NCR aquaculture Extension specialists, economists well known to NCR aquaculture producers, and social license experts. The goal is to develop and test strategies designed to enhance social license for aquaculture in the NCR. A comprehensive study of processing fish in local facilities for local food systemsis funded entirely (2years) from the 2018 grant. This study explores processing fish and other aquaculture products in shared-use commercial kitchen and butcher shop-type facilities in local communities for the growing local food systems. At the time of this report, activities are in the early stages for three projects initated in 2020. New projects and their goals are: Evaluating novel methods for preventing Aeromonas-associated losses in Yellow Perch (Perca flavescens) using laboratory and field-based vaccination trials.Yellow Perch (Perca flavescens; YP) is a priority species within the North Central Region (NCR), and yet few resources have been devoted to improving their health on farms, a matter complicated by the apparent emergence of several YP-pathogenic Aeromonas spp. across the upper Midwest. This project will evaluate the a novel approach for immersion vaccinating young (<1-week-old) salmonids against Aeromonas salmonicida, without handling the fry (i.e., no handling stress). Non-Lethal Bird Deterrent Evaluation in the NCR.This project seeks to evaluate a new non-lethal method of deterring avian predators that relies on disrupting communications among birds, which in turn makes the local area uninhabitable even when an abundance of food is available. Assessing the Effectiveness of NCRAC Funded Research in Aquaculture Within the North Central Region. TThe goal is toto conduct a thorough review of all completed NCRAC funded research and Extension projects from 1994 to 2019, to identify the outcomes, impacts and deliverables generated by each project for producers within the region. The following are updates on projects previously noted as being funded from this project. Genetically Improved All-Female Walleye (Sander vitreus) for Intensive Aquaculture Production in the Great Lakes Region.?Gynogenetic progenies were not produced in 2020 as a result of the Covid-19 state-wide shutdowns in Ohio and Wisconsin. The shutdown limited OSU research capabilities and eliminated sampling efforts of Ohio Department of Natural Resources, from which we receive walleye gametes.Triploid walleye were produced in April 2019. Investigatorsevaluated effectiveness of three different pressure shocks applied 4mpf: 7,000psi, 40-minute duration; 8,000psi, 30 minute; and 9,000psi,12 minute. Triploidy induction rate and embryonic survival were highest in the 9,000psi group (95% and 17.5%). Control, diploid walleye were 100% diploid, with eyed-stage embryonic survival of 11.4%. Itis likely that a growth advantage will not be seen until control, diploid fish begin to sexually mature and grow the gonads, while it is likely that triploid individuals will not mature sexually, instead, continuing to invest energy in body growth. Nutritional Programming of Yellow Perch Larvae Using Live Food as a Vehicle.The preliminary results from the largemouth bass (substitute for yellow perch that were not available [pandemic restratints] study suggests that the timing of nutritional programming and method for soybean meal deliveryseems critical to improve dietary plant proteinutilization. Although programming during the first feeding using live food-enriched soybean meal is a practical way of inducing programming and leads overall to the highest survival and fish size as opposed to dry feed programming, the latter results in a better utilization of dietary soybean meal during the grow-out. However, further investigation is still needed to provide a recommendation on the most beneficial NP approach and will be included in the final report. Optimization of Practical Feed Formulation to Improve Fish Health and Production of Yellow Perch (Perca flavescens). Similar to other funded projects, progress on this project was delayed to ongoing pandemic issues.To prepare for the upcoming trial, PIs successfully spawned some fish in August, 2020 using out-life cycle broodstocks and now have fingerlings available at University of Wisconsin-Milwaukee. A feeding trial will start as soon as we can get feed manufactured by our Co-PI. We also had difficulties in getting ingredients for feed preparation but expect that the test feed will be made during the last week of December, 2020 or the first week of January, 2021. A feeding trial will start in January, 2021 as soon as the feed are ready.Initial finds from this study indicate that under the current testing conditions, wheat flour promoted a better growth in yellow perch juvenile than corn flour but it also tended to induce lipid accumulation in liver tissue or whole fish. The diets containing 25% wheat or corn flour did not depress growth when compared the diet containing 15% flour. It was unexpected that the fish fed 20% flour had the lowest growth rate.

Publications

  • Type: Other Status: Published Year Published: 2021 Citation: 2020 North Central Regional Aquaculture Center Annual Report. Available at https://www.ncrac.org/files/page/files/2020_annual_report_draftc.pdf.
  • Type: Theses/Dissertations Status: Accepted Year Published: 2020 Citation: Miller, M. 2020. Induction of female monosex polyploid Yellow perch (Perca flavescens) and production of monosex stocks in order to increase efficiencyof Yellow perch aquaculture. Dissertation. Not yet available online. Ohio State University, Columbus, Ohio. (December 13, 2020).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Quinlin, K. 2020.Undergraduate research experience  non-thesis. Poster presentation at Denman Research Forum. The effect of fermented fish waste-based diets on spawning and reproduction of zebrafish (Danio rerio)  model studies for aquaculture. Ohio State University, Columbus, Ohio. (May 2020).
  • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: Haley M. Lucas, 2020. Analysis of plant biomass production comparing decoupled aquaponics against equivalent single-loop aquaponic and hydroponic systems growing Lactuca sativa. University of Wisconsin-Milwaukee, Milwaukee, Wisconsin. Not yet available online (August 19, 2020).
  • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: Sonya C. Ponzi, 2020. Evaluation of spermcryopreservation for yellow perch (Perca flavescens) broodstock management. University of Wisconsin-Milwaukee, Milwaukee, Wisconsin. Not yetavailable online (December 19, 2020).


Progress 09/01/19 to 08/31/20

Outputs
Target Audience:The North Central Regional aquaculture community includes both established aquaculturists and those interested in aquaculture as part of their agriculture diversification. In addition, supporting industries, i.e., commercial fish feed producers and manufacturers,and fish wholesalers and retailers, University and agency personnel (research and extension/outreach), and federal/state/local government officials. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?In all three identified projects, there is a combination of undergraduate and graduate students being trained in aquaculture as part of their involvement in project development. Examples of this training include cryopreservation methods for walleye sperm, use of enriched live foods for larval fish, and training students in basic aquaculture. How have the results been disseminated to communities of interest?Although three new projects were initiated in July 1, 2019, initial activities were communicated among the NCRAC community members during the 2020 NCRAC annual meeting. All projectsare on the current NCRAC web site for public information. The NCRAC administrative staff have continued to refine theNCRAC web site to allow for easier information transfer to all members of the NCRAC community, i.e., fact sheets, videos andannual reports. Also, this web site has continued to be updated in January 2020with current rules and regulations by stateand federal agencies as well as time senstive announcements. What do you plan to do during the next reporting period to accomplish the goals?In addition to the next annual report for the three current research projects (initiated in July 2019), identified fact sheets and workshops will be developed by the funded research and extension staff with guidance by the NCRAC adminstrative unit. It is anticipated that there will be increased use of web-based portals for content delivery to the aquaculture community of information garnered by the new projects as well as those projects that continuing on from previous grants.

Impacts
What was accomplished under these goals? The research activities funded by the North Central Regional Aquaculture Center (NCRAC) over the period of this project are generating new technologies and publications of benefit to the aquaculture industry, by increasing production, decreasing costs, and by providing information that will lead led to more knowledgeable decisions. At the time of this report, activities are in the early stages for three projects initiated on July 1, 2019. New projects and their goals at NCRAC are: GeneticallyImproved All-Female Walleye (Sander vitreus) for Intensive Aquaculture Production in the Great Lakes Region (to usegenetic methods to produce triploid walleye Sander vitreus that will result in superior growth of the female genotype (30%growth advantage over males) and avoidance of nutrients expenditure associated with sexual maturation by polyploidy(sterilization); Nutritional Programming of Yellow Perch Larvae Using Live Food as a Vehicle (contribute to expansionof Yellow Perch production and development of competitive aquafeed market within the North Central Region (NCR) byproviding feed manufacturers and farmers with possibility of using bigger raw material basket allowing for more flexibility informulations of diets deprived of fishmeal; and Optimization of Practical Feed Formulation to Improve Fish Health andProduction of Yellow Perch (Perca flavescens) (to increase the profitability of yellow perch aquaculture by developingnutritionally balanced and cost effective feed). This grant will fund the second year of each of these three grants. The NCRAC annual meeting was in Columbus, Ohio, on January 30-31, 2020; the last day was a 1-day Listening Session on January 31.Presentations for the NCRAC annual meeting were made by NCRAC-funded PIs concerning activitiesthis project period,updating the NCRAC database of researchers, extension and private industry members;preparation of the NCRAC annual report and completing annual reports; updating the NCRAC web site; and distributingpublications received from the other four Centers to the extension community of the North Central Region. The two goals for the Listening Session were: to develop effective communication between research and industry, which we believe was achieved and to use the feedback in the listening sessions to optimize and clarify the direction to the future submittal of pre-proposals by the research and extension community. The RFP2021 was developed with guidance to PIs to use this information in developing their proposals. Information on this Listening Session is available athttps://www.ncrac.org/ncrac-2020-conference-listening-sessions-summary. The 2020conference was hosted in Columbus, Ohio(200+attendees). There were54 presentations spread across 18 sessions, including two sessions titled "Real World Farmer Experiences" where producers highlighted their journey as they grow aquatic species.Presenters included a number of NCRAC-funded researchers and extension specialists as well as regional industry members. Presentations from the 2014, 2016 and 2018 conferences can befound at https://www.ncrac.org/presentations; presentation from the 2020 conference has been delayed due to Covid-19 concerns.

Publications

  • Type: Other Status: Published Year Published: 2020 Citation: 2019 North Central Regional Aquaculture Center Annual Report. Available at https://www.ncrac.org/files/page/files/2019_annual_report.pdf.


Progress 09/01/18 to 08/31/19

Outputs
Target Audience:The North Central Regional aquaculture community includes both established aquaculturists and those interested in aquaculture as part of their agriculture diversification. In addition, supporting industries, i.e., commercial fish feed producers and fish whoesalers and retailers, University and agency personnel (research and extension/outreach), and federal/state/local government officials. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Throughout the evolution of this project associated fact sheets and education materials are being developed for public distribution. New training methods using Webinars and web-based information were presented to the public. In 2020 there will be a one-day meeting in Columbus, Ohio that will be used to present and collect information on regional aquaculture needs of the industry, research and extension/outreach communities. How have the results been disseminated to communities of interest?Although threenew projects were initiated in July 1, 2019, initial activities were communicated among the NCRAC community members with the first annual report prepared for distribution noted to the NCRAC community in January 2019. All projects areon the current NCRAC web site for public information. The NCRAC administrative staff have continued to refine the NCRAC web site to allow for easier information transfer to all members of the NCRAC community, i.e., fact sheets, videos and annual reports. Also, this web site has continued to be updated in February 2019with current rules and regulations by state and federal agencies as well as time senstive announcements. What do you plan to do during the next reporting period to accomplish the goals?In addition to the next annual report for the threecurrent research projects (initiated in July 2019), identified fact sheets and workshops will be developed by the funded research and extension staff with guidance by the NCRAC adminstrative unit. It is anticipated that there will be increased use of web-based portals for content delivery to the aquaculture community of information garnered by the new projects as well as those projects that continuing on from previous grants.

Impacts
What was accomplished under these goals? The research activities funded by the North Central Regional Aquaculture Center (NCRAC) over the period of this project are generating new technologies and publications of benefit to the aquaculture industry, by increasing production, decreasing costs, and by providing information that will lead led to more knowledgeable decisions. At the time of this report, activies are in the early stages for three projects initiated on July1, 2019. Newprojects and their goals at NCRAC are: Genetically Improved All-Female Walleye (Sander vitreus) for Intensive Aquaculture Production in the Great Lakes Region (to use genetic methods to produce triploid walleye Sander vitreus that will result in superior growth of the female genotype (30% growth advantage over males) and avoidance of nutrients expenditure associated with sexual maturation by polyploidy (sterilization)).;Nutritional Programming of Yellow Perch Larvae Using Live Food as a Vehicle (contribute to expansion of Yellow Perchproduction and development of competitive aquafeed market within the North Central Region (NCR) by providing feed manufacturers and farmers with possibility of using bigger raw material basket allowing for more flexibility in formulations of diets deprived of fishmeal; andOptimization of Practical Feed Formulation to Improve Fish Health and Production of Yellow Perch (Perca flavescens) (to increase the profitability of yellow perch aquaculture by developing nutritionally balanced and cost effective feed).This grant will fund the second year of each of these three grants. The Center conducted the annual meeting in Des Moines, Iowa, in February 2018with fournew research projects identified using the new NCRAC project development process. Presentations were made by NCRAC-funded PIs. Additional activities this project period include updating the NCRAC database of researchers, extension and private industry members; preparation of the NCRAC annual report and completing annual reports; updating the NCRAC web site; and distributing publications received from the other four Centers to the extension community of the North Central Region.

Publications

  • Type: Other Status: Published Year Published: 2019 Citation: 2018 North Central Aquaculture Center annual report. Available at https://www.ncrac.org/files/page/files/2018_annual_report_with_cover_final.1_0.pdf