Source: CORNELL UNIVERSITY submitted to
THE INFLUENCE OF CULTURAL MANAGEMENT PRACTICES ON STRAWBERRY YIELD, FLAVOR AND QUALITY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1017299
Grant No.
(N/A)
Project No.
NYC-145323
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 2018
Project End Date
Sep 30, 2021
Grant Year
(N/A)
Project Director
Pritts, MA.
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Horticulture
Non Technical Summary
The marketplace for fruit is becoming very competitive with year-around supplies of most crops available to consumers. Growers who produce the most flavorful fruit will have a competitive edge. New York's primary advantage over other regions has been flavor. However, other regions are paying more attention to flavor and are working to improve the perception that fruit from distant locations is of poorer quality. Certainly, genetics play a major role in flavor development, but undoubtedly the conditions under which fruit is grown can also affect fruit flavor. Considerable debate exists as to what cultural factors affect flavor and quality in fruit crops. While this association has been best explored in grapes with the concept of terroir, similar studies have not been conducted with strawberries which is now a $3 billion industry in the US and $10 billion worldwide. The media often report that organically-grown fruit tastes better, or worldwide. The media often report that organically-grown fruit tastes better, or that highly-fertile soils or plantings treated with pesticides produce fruit with poorer flavor, but comparisons are usually made between one farm and another, and across different varieties, without controlling for the multiplicity of factors that could contribute to differences. Replicated trials in a single location are needed to explore variation in single varieties under different management regimes, but the large size of most fruit crop plants makes such comparisons difficult. Strawberry plants are a perennial fruit crop that are sufficiently small so that different cultural practices can be implemented and replicated on a manageable scale. We will establish 9 management regimes that will allow us to make comparisons of great interest to the fruit-growing community, particularly as it relates to the role of organic matter and soil fertility in the development of flavor and enhancement of yield. We will compare organic and conventional management practices at varying levels of soil organic matter additions, soil nitrogen additions, and pest management intensity to identify any underlying factors that directly impact fruit yield, quality and flavor. The experiment will be conducted on a virgin site and another with a history of strawberry production. At the same time, fruit will be purchased at strawberry farms across the state and evaluated for flavor attributes to estimate the range of variability possible in the traits. Ultimately, we will be able to quantify the range in flavor within a single variety, determine the magnitude of influence that soil management practices have on flavor, and make recommendations on how to improve flavor in strawberries.
Animal Health Component
0%
Research Effort Categories
Basic
0%
Applied
100%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20511221060100%
Knowledge Area
205 - Plant Management Systems;

Subject Of Investigation
1122 - Strawberry;

Field Of Science
1060 - Biology (whole systems);
Goals / Objectives
Our ultimate goal is to identify any underlying relationships between soil microbial dynamics and flavor and quality so these factors can be managed for optimal flavor in an increasingly competitive marketplace. Then we will extend this information to growers with appropriate recommendations. Specifically, we will test the following null hypotheses: 1) Strawberries of the same variety do not different significantly in flavor components across farms in New York State. 2) Conventionally-grown and organically-grown strawberries do not differ in yield, quality or flavor when controlling for nitrogen level and soil organic matter content. 3) The level of management intensity (i.e. quantity of inputs) does not impact yield, quality or flavor. 4) The level of nitrogen availability does not affect yield, quality or flavor. 5) The level of soil organic matter does not affect yield, quality or flavor.
Project Methods
This experiment will utilize Fragaria x ananassa cv. Jewel, a June-bearing cultivar noted for its pleasing flavor. The strawberry plants will be grown outdoors on a silty loam soil. Two fields will be used - one in which strawberries have been growing for the past 4 years and the other which has no recent history of strawberries, thereby reducing the likelihood of soil-borne pathogens from previous strawberry crops. There will be three management systems (organic, conventional, low organic matter) each with three levels of management intensity, each replicated 4 times in a completely randomized design on both sites. Plot size is 120 square feet (8 m2). The set of organic system treatments will be managed using organic practices. Conventional system treatments will be managed using standard practices. The low organic matter system will use conventional practices but without any organic matter additions through cover cropping or use of mulch or compost. Each of these systems will be managed at different levels of intensity. Low intensity systems will receive low nitrogen additions and minimum pesticide applications, regardless of system. The moderate level of intensity will receive standard rates of nitrogen and minimum pesticide additions. The highest level of intensity will receive the standard rate of pesticide additions. The highest level of intensity will receive the standard rate of nitrogen plus calendar-based pesticide sprays. Of course, the nitrogen source and pesticide active ingredients will differ depending on the management system. Comparisons of fruit yield, quality and flavor then can be made across management regime at the same level of applied N, across different levels of N, across different levels of management intensity, and between low and high organic matter systems. Prior to planting in April 2018, a comprehensive soil analysis will be performed by collecting one composite soil sample from each field to provide baseline information for the study. The field soil will be tested again at the end of the first growing season in August 2018, and upon final harvest of strawberries in August 2019. The second and third analyses will quantify soil nutrient availability, microbial respiration, soil organic carbon content, and mineralizable nitrogen. Yields will be quantified and fruit quality assessed in 2019 during the first fruiting season. Flavor will be evaluated by measuring volatile aromatic compounds within the strawberries. Anthocyanin content will be evaluated by measuring absorbance at appropriate wavelengths. Sugar content will be evaluated by juicing five samples of berries from each harvest and measuring with a refractometer. Fragrance will be evaluated by measuring volatile aromatic compounds using SPME GC/MS. Whole, frozen strawberry samples, will be thawed for 90 minutes, have leaves removed, and blended into a puree. 10 g of pureed strawberries will be placed into a 40mL amber-glass vial and combined with 1g of 1% calcium chloride solution, and 100 µL of an internal standard consisting of 3-heptanone, 2-decanol, -undecalactone, d3-IPMP, 2-methyl-4-pentanol, 3-heptanone, 2-decanol, -undecalactone, d3-IPMP, 2-methyl-4-pentanol, d8-napthalene, d2-E2-hexenal, E2-hexenal, and d12-hexanal. A calibration curve will be constructed using volatiles detected in Jewel strawberry samples with known associated aromas, and odor thresholds at or below 100 ppb, such as linalool, nonanal, hexanoic acid, butanoic acid, acetic acid, and ethyl hexanoate. The resulting calibration curve will be integrated against the peaks of the selected compounds detected within the strawberries. A sensory evaluation will be performed by asking tasters to sample three berries from selected treatment levels, two of which will be from the same treatment to determine if differences are detectable. Relationships will be examined between management practices, soil characteristics and fruit quality components.Growers (target is 50 over two years) will be asked to participate in the research by providing a small sample of 'Jewel' strawberry for analysis. Berries will be sorted to the same stage of ripeness and analyzed for sugar content and flavor components. Individual growers will learn where their strawberries fall within the range of sugar content, flavor components and other measures of quality. Correlations will be made between management practices on these farms and these results. Farm visits will provide an opportunity for outreach on soil management and soil health.

Progress 10/01/18 to 09/30/21

Outputs
Target Audience:Primary target audience is commercial fruit growers who want to know how to best manage their crops. Secondary audience is extension educators who will communicate results to growers. Tertiary audience is consumers who are interested in knowing how their food is grown and if growing methods affect fruit flavor. We sampled fruit from 22 farms over the course ofour experiment. These farms were presented with individual results from their farm, and overall trends are currently being presented to the berry industry. Changes/Problems:COVID restrictions required us to request an extension for the project, but we were able to complete the analyses by the end of 2020 and publish results in 2021. What opportunities for training and professional development has the project provided?One graduate student based her M.S. thesis on this project. Extension educators attended conference presentations and read articles about the work. How have the results been disseminated to communities of interest?Three manuscripts were initiated based on findings from this study. Two articles were published in grower-focused publications, andconference presentations and newslettersfindings were prepared. For example, a presentation was made to the New England Vegetable and Fruit Conference with 195 attendees at the session where this material was presented,New Jersey Fruit and Vegetable Growers Annual Meeting, and Minnesota Fruit and Vegetable Growers annual meeting with 54 participants. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We completed analyses from a three-year farm survey and from a two-year research farm trial that required us to measure soluble solids content, titratable acidity, phenolic composition, and aromatic volatiles in strawberry samples from 22 farms across New York State and from 9 experimental treatments. Treatments varied in nitrogen rate, the source of nitrogen, the amount of organic matter within a site, and the use of various microbial supplements and crop protectants. We also monitored soluble solids content (SSC) in day neutral strawberries during the 2020 growing season along with weather variables with the intent of associating temperature patterns during fruit development with final SSC in ripe fruit. This followed from our farm survey which identified fruit exposure to warm days and cool nights as the principal driver of sugar content in ripe fruit and was designed to validate observations in the short-day strawberry farm survey. Aroma varied greatly across farms and treatments but was not associated with the factors we measured. Strawberry production methods affected yield (especially nitrogen rate) but did not appear to influence flavor development. The most significant determinant of flavor in strawberries appears to be their exposure to warm days and occasionally cool nights during fruit development, and not by the way strawberries are grown, or the inputs used to grow them. This finding can potentially save growers money by not spending on inputs that will not affect flavor. This was corroborated by study of day neutral strawberries in 2021 where the same trends were observed (cool nights and warm days were associated with higher sugar content in fruit). One M.S. thesis was completed through this project, and three manuscripts were initiated based on findings from this study. Two articles were published in grower-focused publications, and conference presentations and newsletters findings were prepared.

Publications

  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Osatuke, A. and M. Pritts. 2021. Development of quality attributes in strawberry fruit: A review. J. Amer. Pomol. Soc. 75:50-62.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Osatuke, A. and M. Pritts. 2021. Strawberry flavor is influenced by the air temperature differential during fruit development but not management practices. Agronomy 11: 606. https://doi.org/ 10.3390/agronomy11030606
  • Type: Other Status: Published Year Published: 2021 Citation: Osatuke, A. and M. Pritts. 2021. Can strawberry flavor be influenced by growing practice? New York Fruit Quarterly 29 (1):9  12.
  • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: SOIL MANAGEMENT AND SITE AND THEIR EFFECTS ON STRAWBERRY FRUIT QUALITY AND YIELD Anya Osatuke, M.S. thesis, Cornell University
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Osatuke, A. and M. Pritts. 2021. Sugar concentration in strawberry is influenced by temperature during fruit development. J. Amer. Pomol. Soc. 75:134-140.
  • Type: Other Status: Published Year Published: 2021 Citation: Osatuke, A. and M. Pritts. 2021. Temperature patterns during fruit development determine sugar content in ripe fruit. New York Fruit Quarterly 29 (2):32-34.


Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Primary target audience is commercial fruit growers who want to know how to best manage their crops. Secondary audience is extension educators who will communicate results to growers.Tertiary audience is consumers who are interested in knowing how their food is grown and if growing methodsaffect fruit flavor. We sampled fruit from 22 farms over the course of our experiment. These farms were presented with individual results from their farm, and overall trends are currently being presented to the berry industry. Changes/Problems:Due to COVID restrictions, our access to lab facilities was restricted so we had to freeze samples and were not able to analyze them until later than we projected. Farm visits were limited because we could not stay overnight during sampling. We could not interact with growers to communicate our results directly to them. The experiments and deliverables will becompleted, but just delayed. What opportunities for training and professional development has the project provided?One graduate student based her M.S. thesis on this project. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Finish the chemical analysis of strawberries (currently frozen) Publish a summary of our finding in the New York Fruit Quarterly, a publication primarily read by farmers. Publish an article about ourwork in the New York Berry Growers newsletter. Present findings at grower conferences this coming winter. Publish threemanuscripts in a scientific journals: 1) A review article on factors that influence strawberry flavor, 2) A research article on factors that influence strawberry flavor, and 3) A research article on how temperature patterns during development affect sugar content in strawberries

Impacts
What was accomplished under these goals? We completed the vast majority of analyses from a three-year farm survey and from a two-year research farm trial that required us to measure soluble solids content, titratable acidity, phenolic composition, and aromatic volatiles in strawberry samples from 22 farms across New York State and from 9 experimental treatments. Treatments varied in nitrogen rate, the source of nitrogen, the amount of organic matter within a site, and the use of various microbial supplements and crop protectants. We also monitored soluble solids content (SSC) in day neutral strawberries during the 2020 growing season along with weather variables with the intent of associating temperature patterns during fruit development with final SSC in ripe fruit. This followed from our farm survey which identified fruit exposure to warm days and cool nights as the principal driver of sugar content in ripe fruit, and was designed to validate observations in the short-day strawberry farm survey.Aroma varied greatly across farms and treatments, but was not associated with the factors we measured. Strawberry production methods affected yield, but didnot appear to influence flavor development. The most significant determinantof flavor in strawberries appears to be their exposure to warm days and occasionally cool nights during fruit development. One M.S. thesis was completed through this project, and three manuscripts were initiated based on findings from this study.We also are in the process of preparing conference presentations and newsletters based on the findings.

Publications


    Progress 10/01/18 to 09/30/19

    Outputs
    Target Audience:Strawberry growers are interested in knowing if the way they grow their plants can influence flavor. This project is targeted to commercial strawberry growers. Several strawberry growers participated in this research by providing fruit samples. In addition, the project involved five undergraduate interns who received training in how to conduct field research. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project was the basis of a MS project for one student. This project provided training for five undergraduates interested in fruit research. How have the results been disseminated to communities of interest?Results from the first year have just now been analyzed so we anticipate extension of information to the target audience in 2020. What do you plan to do during the next reporting period to accomplish the goals?We will harvest fruit for one more year to measure longer-term effects of these treatments on yield. We will report this year's findings to grower audiences. A report to the NY Berry Growers Association and North American Strawberry Growers Association has been prepared. Speaking engagements at grower conferences have been set. An article will be written for the NY Fruit Quarterly. After 2020 data are collected, we will submit a manuscript for publication.

    Impacts
    What was accomplished under these goals? After growing strawberry plants (cultivar Jewel) for nearly two years under nine different management regimes, we harvested fruit for the first time in June-July. These fruit were analyzed for yield, size, sugar content, acid content, phenolic content and flavor volatiles. In addition, taste and sniff tests were performed with volunteers to determine if they could distinguish fruits produced under different treatments. Lastly, strawberries were harvested from different farms across New York State and subjected to the same analyses described above. We found differences in flavor attributes among farms within the state. We also found that treatments with higher inorganic nitrogen applications increased both foliar nitrogen and yield, but flavor attributes were not affected significantly. Organic sources of nitrogen did not increase yield, despite equivalent amounts applied across treatments. Despite differences in growing methods, flavor attributes were not changed significantly, nor could humans distinguish among treatments. These findings suggest that modest changes in growing methods will not impact flavor development in a single cultivar on an individual farm with the same soil type, but can impact yield.

    Publications