Progress 09/01/18 to 08/31/23
Outputs
Target Audience:This USDA-funded project has significantly contributed to the educational and research experiences of a diverse group of students at FSU. Key highlights include: (1) One Ph.D. student (Fall 2021) and one M.S. student (Summer 2021) partially supported by this project successfully graduated. (2) A laboratory assistant contributed to the project from February to April 2022. (3) The Food Safety Summer Internship program was offered exclusively to FSU students. Six undergraduate students and one M.S. student participated in a six-week research program (10 hours/week) during Summer 2019, under the supervision of the Principal Investigator (PI) and graduate students. (4) Two undergraduate students joined through InternFSU, and one via the FSU Undergraduate Research Opportunity Program (UROP) in 2019, collaborating closely with a Ph.D. student and two M.S. students on the project. ? Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project enhanced both undergraduate and graduate students' expertise in the areas of food safety with a focus on fish allergen characterization and detection. It has played a pivotal role in preparing them for the professional job market. Key aspects of their training included: (1) Research skills: Students gained hands-on experience in designing and conducting experiments and data analysis. (2) Scientific writing: Students developed proficiency in drafting conference abstracts and contributing to peer-reviewed journal manuscripts. (3) Presentation skills: Students were trained in preparing and delivering both poster and oral presentations in a scientific manner. How have the results been disseminated to communities of interest?Our project has actively engaged in sharing its findings with relevant communities, ensuring that the results reach and benefit those most interested and impacted. The dissemination strategies included: (1) We have published our findings in peer-reviewed journals. (2) We have presented the research results at international, national, and local conferences, targeting academic and research communities. (3) We have incorporated our findings into educational materials to disseminate relevant information to students. (4) We have disseminated our findings through seminars to reach a broader audience who are interested in food safety and allergy research. (5) We have worked with a local industry partner, contributing to the development of their products through our collaborative efforts. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
(1) Three research articles were published in prestigious peer-reviewed journals including Food Chemistry and Food Control. (2) One review article was featured in Comprehensive Reviews in Food Science and Food Safety. One concise review was published in Foods. (3) One research article is currently under review in Food Chemistry. (4) One Ph.D. dissertation and one M.S. thesis were published. (5) This project has provided scientific training to a significant number of students, including at least six graduate students and eight undergraduate students, thereby contributing to their academic and professional growth. (6) A total of 17 presentations were delivered at various scientific conferences, including 3 international, 13 national, and 1 state-level. Key conferences include IFT (Institute of Food Technologists), IAFP (International Association for Food Protection), ACS (American Chemical Society), and the Association of Official Analytical Collaboration (AOAC) International. (7) Two Ph.D. students garnered 1st place honors in the Aquatic Food Products Division and Toxicology & Safety Evaluation Division Graduate Student Oral Competitions at the 2020 and 2022 IFT Annual Meetings, respectively. (8) Two students secured 1st and 3rd places in the CAFPNA (Chinese Association for Food Protection in North America). (9) Presented several lectures at FSU and the University of Florida, contributing to knowledge sharing in the field.
Publications
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Rao, Q. In vitro immunodetection of fish allergens. Presentation at the University of Florida (virtual), Gainesville, FL.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Jiang, X., Rao, Q. Immunodetection of finfish residues on food contact surfaces. Food Chemistry, 426: 136502. DOI: 10.1016/j.foodchem.2023.136502.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Jiang, X., & Rao, Q. Detection of finfish residues on contact surfaces by enzyme-linked immunosorbent assay. Presentation at the 2023 ACS Spring Annual Meeting, Indianapolis, IN.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Rao, Q., Jiang, X., Tang, C., Zhao, Y., & Tan, J. Application of novel non-thermal processing technologies in food protein analysis. Presentation at the 2023 IAFP Annual Meeting, Toronto, Canada.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Jiang, X., & Rao, Q. Development and characterization of ssDNA aptamers against finfish proteins. Presentation at the 2023 IFT Annual Meeting, Chicago, IL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Zhao, Y., Jiang, X., & Rao, Q. Enzyme-linked immunosorbent assay for the detection of allergenic bony fish residues. Presentation at the 2023 IFT Annual Meeting, Chicago, IL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Zhao, Y., Jiang, X., & Rao, Q. Competitive ELISA for the detection of allergenic fish residues. Presentation at the 2023 AOAC Annual Meeting, New Orleans, LA.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Rao, Q., Tang, C., Zhao, Y., & Tan, J. Effect of non-thermal processing on physicochemical and immunochemical properties of seafood proteins. IFT-EFFoST International Nonthermal Processing Workshop, Minneapolis, MN.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Rao, Q. In vitro immunodetection of fish allergens. 2023 International Webinar on Food Processing and Safety, Xiamen, China.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2023
Citation:
Zhao, Y., Jiang, X., Bai, C., Xia, X., & Rao, Q. Monoclonal antibody-based competitive enzyme-linked immunosorbent assay for the quantification of bony fish tropomyosin. Food Chemistry.
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Progress 09/01/21 to 08/31/22
Outputs
Target Audience:One Ph.D. student partially supported by this USDA-funded project graduated in Fall 2021. One laboratory assistant worked on this USDA-funded project from February to April 2022. Changes/Problems:From January 2022 to April 2022,due to building safety concerns and related building renovations, the accessibility to the laboratory was limited. Starting in May 2022, the project can be continued in thislaboratory. What opportunities for training and professional development has the project provided?This project enriched students' learning of fish allergens and protein chemistry. The graduate students were trained in making research notes, completing experiments, collecting data, conducting data analysis, and writing/presenting scientifically. How have the results been disseminated to communities of interest?One review article was published in Comprehensive Review in Food Science and Food Safety. One research article was accepted for publication in Food Chemistry. One oral presentation on parvalbumin purification and aptamer development against fish proteins was given at the IAFP (International Association for Food Protection) Annual Meeting and was selected for the Developing Scientist Award Competition. One poster presentation on cod tropomyosin assay development was given at the IFT (Institute of Food Technologists) Annual Meeting and won first place in the Toxicology & Safety Evaluation Division Graduate Student Oral Competition. One poster presentation on the effect of non-thermal processing on seafood antigenicity was given at the IFT Annual Meeting and was selected as one of the finalists in the Nutraceutical and Functional Foods Division Graduate Student Oral Competition. In addition, two oral and one poster presentations were given at the CAFPNA (Chinese Association for Food Protection in North America) Student Presentation Competition and the FAFP (Florida Association for Food Protection) Annual Education Conference, respectively. One oral presentation was given at the 6th International Symposium on Food and Health (virtual). What do you plan to do during the next reporting period to accomplish the goals?At least two research manuscripts will be submitted/published in a prestigious peer-reviewed journal. The undergraduate/graduate students involved in this project will present their research findings at local, regional, or national conferences.
Impacts
What was accomplished under these goals?
One USDA-funded Ph.D. student graduated in Fall 2021, who worked on aptamer development for the detection of fish proteins. Briefly, single-stranded DNA aptamers against fish proteins were developed using microplate systematic evolution of ligands by exponential enrichment (SELEX), and their selectivity and structure were characterized.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Jiang, X., Zhao, Y., Tang, C., Appelbaum, M., Rao, Q. Aquatic food animals in the U.S.: Status quo and challenges. Comprehensive Reviews in Food Science and Food Safety, 21(2), 1336-1382.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Zhao, Y., Jiang, X., Tang, C., Rao, Q. Composition, structural configuration, and antigenicity of Atlantic cod (Gadus morhua) tropomyosin. Food Chemistry, 399, 133966
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2021
Citation:
Jiang, X. Characterization and detection of parvalbumin from four finfish species. Florida State University, College of Health and Human Sciences, Department of Nutrition and Integrative Physiology, Tallahassee, FL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Jiang. X., Rao, Q. Purification and characterization of parvalbumin from four finfish species. Presentation at the 6th International Symposium on Food and Health (virtual), Shanghai, China.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2021
Citation:
Zhao, Y. Monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of finfish tropomyosin. Florida State University, College of Health and Human Sciences, Department of Nutrition and Integrative Physiology, Tallahassee, FL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Zhao, Y., Jiang, X., Rao, Q. Monoclonal antibody-based competitive enzyme-linked immunosorbent assay for the detection of finfish tropomyosin. Presentation at the 2022 IFT Annual Meeting, Chicago, IL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Jiang. X., Tan, J., Rao, Q. Effect of non-thermal processing on seafood antigenicity. Presentation at the 2022 IFT Annual Meeting, Chicago, IL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Jiang. X., Zhao, Y., Rao, Q. Characterization and detection of finfish parvalbumin. Presentation at the 2022 IAFP Annual Meeting, Pittsburgh, PA.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Zhao, Y., Jiang. X., Rao, Q. Characterization and detection of Atlantic cod (Gadus morhua) tropomyosin, a finfish allergen. Presentation at the 2022 FAFP Annual Education Conference, Orlando, FL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Zhao, Y., Jiang. X., Rao, Q. Immunodetection of finfish in non-finfish food products. Presentation at the 2022 Virtual CAFPNA Student Presentation Competition.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Jiang. X., Tang, C., Tan, J., Rao, Q. Application of novel non-thermal processing technologies in seafood protein analysis. Presentation at the 2022 Virtual CAFPNA Student Presentation Competition.
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Progress 09/01/20 to 08/31/21
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project enriched students' learning in finfish allergy and protein chemistry. The graduate students were trained in making research notes, completing experiments, collecting data, conducting data analysis, and writing/presenting scientifically. How have the results been disseminated to communities of interest?One journal article was published in Foods. One manuscript was currently under review in Comprehensive Review in Food Science and Food Safety. Two poster presentations related to the purification, characterization, and detection of fish allergens (parvalbumin and tropomyosin) were given. One of them was selected as the finalist in the Aquatic Food Products Division Graduate Student Research Paper Competition at the 2021 IFT Virtual Annual Meeting. What do you plan to do during the next reporting period to accomplish the goals?At least two research manuscripts will be submitted/published to a prestigious peer-reviewed journal. The undergraduate/graduate students involved in this project will present their research findings at local, regional, or national conferences.
Impacts
What was accomplished under these goals?
One USDA-funded M.S. student graduated in the 2021 Summer. Briefly, tropomyosin from Atlantic cod (Gadus morhua) was purified and characterized. Three monoclonal antibodies (mAbs) targeted at tropomyosin were studied. Two assays (sandwich and competitive enzyme-linked immunosorbent assays (ELISAs) were established to detect finfish tropomyosin in non-finfish products. One Ph.D. student was working on aptamer development against finfish parvalbumin. Briefly, parvalbumin from Atlantic cod, Atlantic salmon (Salmo salar), striped mullet (Mugil cephalus), and tilapia (Oreochromis niloticus) was purified. The conformation and immunoreactivity of parvalbumin were studied using native gel electrophoresis and mAb-based immunoblot, respectively. Aptamers against Atlantic cod parvalbumin are underdevelopmentusing microplate SELEX.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Jiang, X.; Rao, Q. Effect of Processing on Fish Protein Antigenicity and Allergenicity. Foods 2021, 10, 969. DOI: 10.3390/foods10050969.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2021
Citation:
Zhao, Y., Jiang, X., Rao, Q. Monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for finfish tropomyosin detection. Presentation at the 2021 Virtual IFT Annual Meeting.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2021
Citation:
Jiang. X., Rao, Q. Purification and characterization of fish parvalbumin, a major food allergen. Presentation at the 2021 Virtual IFT Annual Meeting.
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Progress 09/01/19 to 08/31/20
Outputs
Target Audience:An undergraduate paid internship through the InternFSU program was led by the PIand coordinated by one Ph.D. student and two M.S. students. The information about intern recruitment was released via FSU NoleNetwork. Finally, more than 25 applications were received from 9 colleges and 15 majors. After conducting the interview and careful consideration, two students were selected to join this Food Safety Research Internship. Among these two students, there was one white and one Hispanic. Besides, another undergraduate student was recruited through the FSU Undergraduate Research Opportunity Program (UROP). This undergraduate student worked closely with the Ph.D. student on this USDA-funded project. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project gave an internship opportunity to enrich students' learning in food safety and develop better-prepared students for a growing job market. The training includes how to make research notes, complete research experiments, collect data, conduct statistical analysis, contribute to conference abstracts and peer-reviewed journal manuscripts, and prepare poster/oral presentations scientifically. How have the results been disseminated to communities of interest?One journal article was published in Food Control. A poster presentation related to anti-fish allergen antibody characterization was given. This poster presentation won first place in the Aquatic Food Products Division Graduate Student Research Paper Competition at the 2020 IFT Virtual Annual Meeting. What do you plan to do during the next reporting period to accomplish the goals?At least one research paper and one comprehensive review will be submitted/published to a prestigious peer-reviewed journal. The undergraduate/graduate students involved in this project will present their research findings at the local, regional, or national conferences.
Impacts
What was accomplished under these goals?
Two M.S. students were recruited in Fall 2019. A Food Safety Research Internship was held in Fall 2019. Two undergraduate students conducted some research activities under the guidance of the PI and two M.S. graduate students. In addition, another undergraduate student worked closely with one Ph.D. student on this project. Parvalbumin from several fish species, including cod, mullet, and salmon, were successfully purified. Six mAbs specific to different seafood proteins were studied. One journal article was published in Food Control. A poster presentation related to anti-fish allergen antibody characterization won the first place in the Aquatic Food Products Division Graduate Student Research Paper Competition at the 2020 Virtual IFT Annual Meeting. In addition, the PI attended the NLGCA 2019-Biennial Project Director's Meeting (San Angelo, TX) in October 2019 and gave a poster presentation.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Keshavarz, B., Rao, Q. C., Jiang, X. Y., & Hsieh, Y.-H. P. Immunological analysis of pepsin-digested fish tropomyosin. Food Control, 118, 107427. DOI:10.1016/j.foodcont.2020.107427.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Rao, Q. C., Keshavarz, B., & Jiang, X. Y. Identification of a new potential allergen from mullet and salmon. Presentation at the 2019 AOCS Annual Meeting and Expo, St. Louis, MO.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Jiang. X. Y., Zhao, Y. Q., & Rao, Q. C. Monoclonal antibodies specific to seafood proteins to fight seafood fraud. Presentation at the 2020 Virtual IFT Annual Meeting.
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Progress 09/01/18 to 08/31/19
Outputs
Target Audience:A Food Safety Research Internship was held in Summer 2019. The internship was lead by the PI (Dr. Qinchun Rao) and coordinated by two Ph.D. students (Ms. Xingyi Jiang and Ms. Tengfei Li). A flyer of the intern recruitment was disseminated across the campus through teaching assistants, college, and university career liaisons. Finally, more than 20 applications were received from four colleges with seven majors. After careful consideration, six undergraduate students and one graduate student were selected to conduct this 6-week internship. Among these seven students (2 males and 5 females), there were four White, one Black or African American, one Asian, and one Hispanic. In addition, two MS students were recruited in Fall 2019 through the financial support from this project. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project gave an internship opportunity to enrich students' learning in food safety and develop better-prepared students for a growing job market. The training includes how to make research notes, complete research experiments, collect data, conduct statistical analysis, contribute to conference abstracts and peer-reviewed journal manuscripts, and prepare poster/oral presentations in a scientific manner. In addition, two MS students were recruited in Fall 2019 through the financial support from this project. How have the results been disseminated to communities of interest?After the 6-week internship, an exit survey was conducted to collect the feedback from all the interns. An oral presentation related to fish allergy has been given at a national conference. What do you plan to do during the next reporting period to accomplish the goals?The second Food Safety Research Internship will be held in 2020 Summer. At least one research paper will be submitted/published to a prestigious peer-reviewed journal. The undergraduate/graduate students involved in this project will present their research findings at the local, regional or national conferences.
Impacts
What was accomplished under these goals?
Through this year, a Food Safety Research Internship was held in 2019 Summer. Six undergraduate students and one graduate student conducted a study on fish allergy under the guidance of the PI and two Ph.D. students. Screening of available monoclonal antibodies (mAb) was performed. The potential mAbs that can be used in assay development were identified. One of the selected mAb was purified and characterized. The research design of parvalbumin purification and aptamer development is in-progress.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Rao, Q. C., Keshavarz, B., & Jiang, X. Y. (presented 2019, May). Identification of a new potential allergen from mullet and salmon. Presentation at the Annual Meeting and Expo, AOCS, St. Louis, MO.
- Type:
Conference Papers and Presentations
Status:
Submitted
Year Published:
2019
Citation:
Jiang. X. Y., Zhao, Y. Q., & Rao, Q. C. (submitted). Monoclonal antibodies specific to seafood proteins to fight seafood fraud. Submitted to the 2020 IFT Annual Meeting, Chicago, IL.
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