Source: UNIVERSITY OF ARKANSAS submitted to NRP
BREEDING AND GENETICS OF SMALL FRUITS AND GRAPES IN ARKANSAS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1016466
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2018
Project End Date
Sep 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
Horticulture
Non Technical Summary
This project primarily is focused on developing small fruit, primarily blackberry, and grape cultivars. Blackbery emphasis will be placed on erect plant growth habit, primocane fruiting, high productivity, good fresh and processed quality, large fruit size, firm fruit for shipping ability, small seed size, tolerance to low winter temperatures, heat tolerance of primocane-fruiting types, and resistance to major diseases. Grape development will focus on: 1.high quality, attractive, hardy, seedless table grapes, with adequate shelf life and ability to be utilized in local markets; 2. adapted wine grape selections for processing quality and viticulture adapted to Arkansas; 3. adapted, enhanced quality, winter hardy muscadine cultivars adapted to the regions of Arkansas from the Arkansas River Valley and southward.Molecular genetic investigations on these crops will elucidate new information on important fruit and plant characters and to use that information to expedite breeding progress.
Animal Health Component
90%
Research Effort Categories
Basic
10%
Applied
90%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2021130108140%
2021129108150%
2021131108110%
Goals / Objectives
1. To develop both thorned and thornless (with primary emphasis on thornless) blackberry cultivars adapted to the upper South which are suitable for hand harvest. Emphasis will be placed on erect plant growth habit, primocane fruiting, high productivity, good fresh and processed quality, large fruit size, firm fruit for shipping ability, small seed size, tolerance to low winter temperatures, heat tolerance of primocane-fruiting types, and resistance to major diseases. 2. To develop adapted, high quality, attractive, hardy, seedless table grapes, with adequate shelf life and ability to be utilized in local markets.3. To test existing wine grape selections for processing quality and viticulture adapted to Arkansas.4. To develop adapted, enhanced quality, winter hardy muscadine cultivars adapted to the regions of Arkansas from the Arkansas River Valley and southward.6. To conduct molecular genetic investigations on these crops to elucidate new information on important fruit and plant characters and to use that information to expedite breeding progress.
Project Methods
Hybridizations will be continued on blackberries and muscadine grapes using as parent material the most promising clones available. New genetic acquisitions will be made as they become available and evaluated for genes of potential value in breeding. Seed germination procedures previously shown to ensure maximum seedling emergence will be used. Seedlings will be grown to fruiting age and evaluated for their potential as new cultivars or for use in further breeding. Promising selections will be established in replicated test plantings in two locations in Arkansas for area adaptability. Advanced selections will be propagated and made available to experiment stations in other states for regional tests. Selections from the table grape breeding effort begun in 1964 will be further evaluated for potential release, although hybridizations will not be continued for this crop.Molecular genetic studies will be conducted, using appropriate experimental procedures, as needed to facilitate and expedite the cultivar development program.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:The primary target audience are small fruit and grape producers who utilize the cultivars. Homeowners often use the cultivars also. The small fruit genetic community will be the primary audience for genetic investations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project has involved five graduate students conduction research on blackberries and muscadine grapes. These students have been involved in the breeding program and are exposed to program activities and management. How have the results been disseminated to communities of interest?New cultivar release information is dissiminated by grower meetings, trade magazines and newsletters, and email/phone contacts. Also, Youtube videos are used to show the cultivars on the web. What do you plan to do during the next reporting period to accomplish the goals?The work in each objective will be continued, with the exception of wine grape selection evaluation as that project area has been completed.

Impacts
What was accomplished under these goals? Cultivars released in fall of 2020 included Dazzle and Indulgence wine grapes and Prime-Ark Horizon blackberry. Filings for protection ahve not been done on these thus not listed in protection section. Breeding activities continued with crossing and selection in blackberry and muscadine grapes. Advanced selection testing continued in blackberry, muscadine grapes and bunch table grapes. We have also expanded molecular breeding and research activities in blackberry. University of Arkansas researchers are collaborating with NCSU (Dr. Hamid Ashrafi and Dr. Gina Fernandez), USDA-ARS (Dr. Nahla Bassil), and United Kingdom researchers on the development of two diploid reference genomes. Our team has completed two years of phenotyping on a blackberry genome-wide association study (GWAS) funded by USDA-AFRI and we are looking forward to analyzing genotype data by the end of 2020. Graduate students have measured thorn density, internode length, fruit size and shape, sweetness, acidity, seed size, firmness, and red drupelet reversion in a panel of ~300 UA breeding selections and cultivars for this GWAS project. We are also working with breeders and scientists at Pairwise, NCSU, USDA-ARS, Cornell, and Plant Sciences, Inc. on a unique public-private partnership to identify and characterize the genetic diversity in a diverse Rubus collection. Other ongoing research on muscadines includes measuring firmness of berries analytically and by sensory, validation of a new candidate gene for bronze berry color, investigations into the inheritance of sex and leaf shape in collaboration with Patrick Conner (UGA), and estimation of genetic diversity of wild and cultivated muscadines across the native range.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Worthington, M.L.* and J.R. Clark. 2020. Development of blackberry cultivars with novel plant architecture. Acta Hort. 1277: 159-164.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Worthington, M.L.*, R. Aryal, N.V. Bassil, D. Mead, G.E. Fernandez, J.R. Clark, F. Fernández-Fernández, C.E. Finn, K.E. Hummer, and H. Ashrafi. 2020. Development of new genomic resources and tools for molecular breeding in blackberry. Acta Hort. 1277: 39-46.


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:The primary target audience are small fruit and grape producers who will utilize the cultivars. Homeowners often use the cultivars also. The small fruit genetic community will be the primary audience for genetic investations. Changes/Problems:No major changes have been made in the program. What opportunities for training and professional development has the project provided?Graduate student and undergraduate students are included in program activities. This includes both field and laboratory activities. How have the results been disseminated to communities of interest?Variety releases are licensed to nurseries for public sale of plants, unrestricted in the US. Presentations and newsletter/stakeholder publications are used to announce the new developments. What do you plan to do during the next reporting period to accomplish the goals?The breeding program in blackberry and muscadine grapes will be continued, with crossing and selection cycles carried out as annually done.

Impacts
What was accomplished under these goals? Ponca blackberry was released. The breeding cycle was completed for the year with crossing and seedlings planted for blackberry and muscdine grape crops. Seedling evaluations yielded numerous selections for further evaluation and parent use.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Clark, J.R., M. Worthington, and T. Ernst. 2019. Caddo thornless blackberry. HortScience 54:16321636.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lewter, J., M. Worthington, J.R. Clark, A.V. Varanasi, L. Nelson, C.L. Owens, P. Conner, G. Gunawan. 2019. High-density linkage maps and loci for berry color and flower sex in muscadine grape (Vitis rotundifolia). Theor. Appl. Genet. 132:15711585.