Progress 09/01/18 to 02/28/21
Outputs
Target Audience:Animal health and feed additive companies, livestock producers, veterinarians, and animal nutritionists will be interested in the outcomes of the research. Successful development of a new class of novel alternatives to antibiotics will have a profound impact on the in-feed antibiotics, antibiotic alternatives, and animal therapeutics markets Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We recently discovered several combinations of natural compounds with a strong capacity to enhance animal intestinal immunity by synergistically inducing the synthesis of a large array of host defense peptides (HDPs) that possess potent antimicrobial and immune regulatory activities. Coupled with their barrier-protective and anti-inflammatory properties, these compounds are promising candidates for further development as next-generation antibiotic alternatives. In this project, we will seek to demonstrate the feasibility of developing these immune boosting compounds as novel antibiotic alternatives for disease control and prevention. Successful completion of the project will lead to identification of a brand-new, first-of-a-kind class of antibiotic alternatives for use in poultry and likely other livestock species as well. Because they act on the hosts but not the pathogens, these natural compounds are not expected to exert selective pressure or provoking microbial resistance. The outcome will pave the way for the development of an urgently needed, hugely promising, and paradigm-shifting technology that meets the FDA mandates as well as public demands for safe, healthy, and environmentally responsible animal products. We continued with functional characterizations of several combinations of natural compounds for their ability to modulate the expression levels of the genes for HDPs, inflammation, and barrier function in live animals. We further assessed their ability to protect chickens from necrotic enteritis and coccidiosis. We have found at least two combinations showed a significant protection of broilers from both enteric diseases. Currently, we are in the process of repeating the animal trials and further evaluate their influence on production performance and meat quality.
Publications
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Progress 09/01/19 to 08/31/20
Outputs
Target Audience:Animal health and feed additive companies, livestock producers, veterinarians, and animal nutritionists will be interested in the outcomes of the research. Successful development of a new class of novel alternatives to antibiotics will have a profound impact on the in-feed antibiotics, antibiotic alternatives, and animal therapeutics markets. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The Co-PD (Dr. G. Zhang) was invited to speak on our antibiotic-alternative strategies in the Symposium on Gut Health in Production of Food Animals between November 4-6, 2019 in St. Louis, MO as well as the 3rd International Symposium on Alternatives to Antibiotics between December 16 -18, 2019 in Bangkok, Thailand. How have the results been disseminated to communities of interest?The Co-PD (Dr. G. Zhang) has presented our latest research advances in national and international research conferences. The results are also being prepared for publication. What do you plan to do during the next reporting period to accomplish the goals?We will continue to follow the project objectives and further evaluate the efficiency of two combinations of natural compounds for their ability to protect animals against necrotic enteritis and coccidiosis, in comparison with an in-feed antibiotic. In addition, we will measure their impact of production performance and meat quality of broilers, paying the way for their commercial development as novel alternatives to antibiotics.
Impacts
What was accomplished under these goals?
We recently discovered several combinations of natural compounds with a strong capacity to enhance animal intestinal immunity by synergistically inducing the synthesis of a large array of host defense peptides (HDPs) that possess potent antimicrobial and immune regulatory activities. Coupled with their barrier-protective and anti-inflammatory properties, these compounds are promising candidates for further development as next-generation antibiotic alternatives. In this project, we will seek to demonstrate the feasibility of developing these immune boosting compounds as novel antibiotic alternatives for disease control and prevention. Successful completion of the project will lead to identification of a brand-new, first-of-a-kind class of antibiotic alternatives for use in poultry and likely other livestock species as well. Because they act on the hosts but not the pathogens, these natural compounds are not expected to exert selective pressure or provoking microbial resistance. The outcome will pave the way for the development of an urgently needed, hugely promising, and paradigm-shifting technology that meets the FDA mandates as well as public demands for safe, healthy, and environmentally responsible animal products. We continued with functional characterizations of several combinations of natural compounds for their ability to modulate the expression levels of the genes for HDPs, inflammation, and barrier function in live animals. We further assessed their ability to protect chickens from necrotic enteritis and coccidiosis. We have found at least two combinations showed a significant protection of broilers from both enteric diseases. Currently, we are in the process of repeating the animal trials and further evaluate their influence on production performance and meat quality.
Publications
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Progress 09/01/19 to 02/29/20
Outputs
Target Audience:Animal health and feed additive companies, livestock producers, veterinarians, and animal nutritionists will be interested in the outcomes of the research. Successful development of a new class of novel alternatives to antibiotics will have a profound impact on the in-feed antibiotics, antibiotic alternatives, and animal therapeutics markets. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The Co-PD (Dr. G. Zhang) was invited to speak on our antibiotic-alternative strategies in the Symposium on Gut Health in Production of Food Animals between November 4-6, 2019 in St. Louis, MO as well as the 3rd International Symposium on Alternatives to Antibiotics between December 16 -18, 2019 in Bangkok, Thailand. How have the results been disseminated to communities of interest?The Co-PD (Dr. G. Zhang) has presented our latest research advances in national and international research conferences. The results are also being prepared for publication. What do you plan to do during the next reporting period to accomplish the goals?We will continue to follow the project objectives and further evaluate the efficiency of two combinations of natural compounds for their ability to protect animals against necrotic enteritis and coccidiosis, in comparison with an in-feed antibiotic. In addition, we will measure their impact of production performance and meat quality of broilers, paying the way for their commercial development as novel alternatives to antibiotics.
Impacts
What was accomplished under these goals?
We recently discovered several combinations of natural compounds with a strong capacity to enhance animal intestinal immunity by synergistically inducing the synthesis of a large array of host defense peptides (HDPs) that possess potent antimicrobial and immune regulatory activities. Coupled with their barrier-protective and anti-inflammatory properties, these compounds are promising candidates for further development as next-generation antibiotic alternatives. In this project, we will seek to demonstrate the feasibility of developing these immune boosting compounds as novel antibiotic alternatives for disease control and prevention. Successful completion of the project will lead to identification of a brand-new, first-of-a-kind class of antibiotic alternatives for use in poultry and likely other livestock species as well. Because they act on the hosts but not the pathogens, these natural compounds are not expected to exert selective pressure or provoking microbial resistance. The outcome will pave the way for the development of an urgently needed, hugely promising, and paradigm-shifting technology that meets the FDA mandates as well as public demands for safe, healthy, and environmentally responsible animal products. We continued with functional characterizations of several combinations of natural compounds for their ability to modulate the expression levels of the genes for HDPs, inflammation, and barrier function in live animals. We further assessed their ability to protect chickens from necrotic enteritis and coccidiosis. We have found at least two combinations showed a significant protection of broilers from both enteric diseases. Currently, we are in the process of repeating the animal trials and further evaluate their influence on production performance and meat quality.
Publications
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Progress 09/01/18 to 08/31/19
Outputs
Target Audience:Animal health and feed additive companies, livestock producers, veterinarians, and animal nutritionists will be interested in theoutcomes of the research. Successful development of a new class of novel alternatives to antibiotics will have a profound impacton the in-feed antibiotics, antibiotic alternatives, and animal therapeutics markets. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?One graduate student attended the Poultry Science Association Annual Meeting between July 15-18, 2019 in Montréal, Québec, Canada and presented a poster entitled "Butyrate and forskolin act synergistically in protecting chickens from necrotic enteritis". It was well received and the student was exposed to the latest advances in the field and had great networking opportunities. How have the results been disseminated to communities of interest?A graduate student attended the Poultry Science Association Annual Meeting between July 15-18, 2019 in Montréal, Québec, Canada and presented a poster entitled "Butyrate and forskolin act synergistically in protecting chickens from necrotic enteritis". Dr. Guolong Zhang, Co-PD of the grant, make four invited presentations both in the US and China: 1) Development of next-generation natural alternatives to antibiotics. Eastman Chemical Company, Kingsport, TN. September 13, 2018; 2) Screening of host intestinal defense mechanisms for compounds as possible alternatives to antibiotics. Health and Stress Advisory Board Meeting, Land O'Lakes, Gray Summit, MO. January 22 - 23, 2019; 3) Development of antibiotic alternatives by modulating animal innate immunity. International Conference on Green Feeds and Animal Product Safety, Wuhan, China. August 4-6, 2019; and 4) Immunomodulatory antibiotic alternatives. Zhejiang Academy of Agricultural Sciences, Hangzhou, China. August 8, 2019. What do you plan to do during the next reporting period to accomplish the goals?We will continue to follow the project objectives and further evaluate the efficiacy of two combinations of natural compounds for their ability to protect animals against necrotic enteritis and coccidiosis, in comparison with an in-feed antibiotic. In addition, we will measure their impact of production performance and meat quality of broilers, paying the way for their commerical development as novel alternatives to antibiotics.
Impacts
What was accomplished under these goals?
FDA has recommended the withdrawal of medically important antibiotics for production practices in the U.S. as of January 2017. Although various alternatives to antibiotics are currently on the market, none is able to match the efficacy of antibiotics. We recently discovered several combinations of natural compounds with a strong capacity to enhance animal intestinal immunity by synergistically inducing the synthesis of a large array of host defense peptides (HDPs) that possess potent antimicrobial and immune regulatory activities. Coupled with their barrier-protective and anti-inflammatory properties, these compounds are promising candidates for further development as next-generation antibiotic alternatives. In this project, we will seek to demonstrate the feasibility of developing these immune boosting compounds as novel antibiotic alternatives for disease control and prevention. Successful completion of the project will lead to identification of a brand-new, first-of-a-kind class of antibiotic alternatives for use in poultry and likely other livestock species as well. Because they act on the hosts but not the pathogens, these natural compounds are not expected to exert selective pressure or provoking microbial resistance. The outcome will pave the way for the development of an urgently needed, hugely promising, and paradigm-shifting technology that meets the FDA mandates as well as public demands for safe, healthy, and environmentally responsible animal products. For Objective 1, we have identified several combinations of natural compounds that are highly efficient in inducing HDP gene expression without triggering inflammation. We have evaluated the ability of several different combinations of the compounds in chicken HTC macrophage cells in the presence of absence of bacterial lipopolysaccharides for their impact on the gene expression levels of multiple HDPs, inflammatory, and barrier function genes. We have narrowed down to three combinations for further evaluation in Objective 2. For Objective 2, we are in the process of assessing three combinations of the compounds in chicken models of necrotic enteritis and coccidiosis. We have found at least two combinations showed a significant protection of broilers from both enteric diseases. Currently, we are in the process of repeating the animal trials and further evaluate their influence on production performance and meat quality.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Deng, Z., J. Wang, W. Lyu, X. Wieneke, R. Matts, X. Ma, and G. Zhang. 2018. Development of a cell-based high throughput screening assay to identify porcine host defense peptide-inducing compounds. Journal of Immunology Research 5492941.
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