Source: WASHINGTON STATE UNIVERSITY submitted to NRP
MOLECULAR PLANT SCIENCES (MPS): PLANT PRODUCTIVITY IN A DYNAMIC ENVIRONMENT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1015621
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Feb 20, 2018
Project End Date
Sep 30, 2022
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
Ag Research Center
Non Technical Summary
This new molecular plant science (MPS) Hatch umbrella project will provide advanced fundamental knowledge in different areas of plant biology that will help to improve crop plants under increasingly challenging environmental conditions. Gaining this basic scientific knowledge will pave the way not only for improving existing but also for identifying new strategies to bioengineer crops. Specifically, detailed insights into primary and secondary metabolisms addressed in the focus area Products/Synthesis would improve production of economically-valuable chemicals in plants. Furthermore, the project will study interactions between roots and their biotic (microbiome) and abiotic components of soil. Understanding these interactions would enable breeding crops for profitable farming on marginal lands. Finally, understanding how plants sense their environment in general and stress in particular addressed in focus area Defense/Signaling will provide critical information for protecting plants from damaging impacts of anticipated global climate changes. In this way, the Hatch umbrella consortium will help solve pressing societal problems related to agriculture.
Animal Health Component
45%
Research Effort Categories
Basic
45%
Applied
45%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2060120106030%
1020110102020%
2030120102030%
2040120102020%
Knowledge Area
206 - Basic Plant Biology; 204 - Plant Product Quality and Utility (Preharvest); 102 - Soil, Plant, Water, Nutrient Relationships; 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants;

Subject Of Investigation
0120 - Land; 0110 - Soil;

Field Of Science
1060 - Biology (whole systems); 1020 - Physiology;
Goals / Objectives
The overarching goal of this Molecular Plant Science (MPS) umbrella project is to advance our understanding of the molecular basis of plant and crop productivity and to describe mechanisms how plants survive and thrive in challenging, changing, and often unpredictable environments. This goal addresses five of the six USDA-NIFA challenge areas (Food Security, Climate Variability and Change, Water, Bioenergy, and Food Safety). Although this MPS umbrella consortium focuses on basic aspects of plant biochemistry, physiology, and molecular biology, the scientific knowledge gained here lays an urgently needed foundation for groundbreaking advances in crop productivity and quality as well as crop environmental robustness. The overall goal of this project will be accomplished by the following objectives: 1. Characterization of potential factors that control architectural dynamics in photosynthetic membranes. 2. Identify the rate-limiting processes of starch synthesis and accumulation. 3. Engineer desaturases from a cyanobacterium and a nematode to efficiently reduce saturates during the synthesis of triacylglycerol, the principal component of seed oils. 4. Cloning, recombinant expression, and biochemical and functional characterization of enzymes participating in flavin biosynthesis in plants and bacteria. 5. Identify and/or introduce biochemical pathways to valuable specialty and commodity chemicals (including medicinals) in plants and algae and identify regulatory point controls affecting their amounts produced from photosynthetically produced carbon capture. 6. Characterize important metabolic pathways (genetic impact/control) in important culinary herbs, aromatic plants, and berries that are involved in bioactive compound production with a particular focus on the pathways that produce the curcuminoids, salvinorins, and flavones and identification of the factors that regulate those pathways. 7. Develop spectral databases to support the rapid identification of isoprenoid natural products from complex mixtures. 8. Elucidation of encoding and decoding mechanisms of intracellular Ca2+ signature into various physiological responses through calmodulin and other Ca2+-binding proteins in plants. 9. In-depth understanding of a role for danger signals during abiotic and biotic stresses. 10. Identification of how genes and pathways regulate plant growth and development and then use breeding, transgenic, and genome editing approaches to change gene activity in crops. 11. Determine signaling processes between plant cells and pathogens using the pea endocarp tissue model and the potato excised stem model. 12. Determine genetic mechanisms responsible for regulation of peroxisome abundance in wheat and importance of peroxisomes for drought tolerance. 13. To understand the mode of action of the C-Ph1 gene to answer fundamental questions related to the process of chromosome pairing and homology search. 14. Molecular, genetic, and biochemical characterization of the BUZZ kinase in B. distachyon and A. thaliana. 15. To provide a fundamental framework to improve productive symbiosis and better nitrogen management of associated crops. 16. Provide a fundamental understanding of the regulation of ammonia synthesis in free-living nitrogen fixing organisms using A. vinelandii as a model system. 17. To study root-knot nematode secretions to identify and characterize root-knot nematode parasitism genes.
Project Methods
Due to the diverse research areas defined in the MPS Hatch umbrella a wide set of different techniques and methods will be employed. The following succinctly summarizes these techniques and methods. 1. For studying ultrastructural dynamics in photosynthetic thylakoid membranes state-of-the-art electron microscopy will be used complemented by a battery of biochemical, biophysical, and functional characterizations and computer modeling. 2. RNAseq and metabolomic approaches as well as a variety of biochemical and molecular biological approaches (proteomic analysis, immunoprecipitates, yeast two-hybrid and BiFC, in situ RT-PCR, knock-out mutations identified by TILLING, RNAseq) will be employed to study changes in gene expression and metabolites in transgenic rice lines and the identification of RNA binding proteins of the cortical endoplasmic reticulum in developing rice endosperm. 3. Transgenic approaches will be employed to supply of reductant to fatty acid desaturases that will boost the production of high-oleic oils. Sophisticated transformation vectors will be used to produce multi-transgenic soybean and canola lines for optimized oil compositions. 4. Cloning, recombinant expression, and biochemical characterization of recombinant enzymes will be performed to modify proteins involved in flavin biosynthesis. 5. Multi-omics (transcriptomics, proteomics and metabolomics) approaches at the whole plant, tissue, cell type, or subcellular level, gene cloning (typically the cDNA), recombinant protein expression, together with conventional biochemical and chemical pathway/intermediate identification are employed to identify how to maximize accumulation of valuable plant secondary and bioactive metabolites. 6. Online resources and published literature will be surveyed to generate novel spectral records for integration into the Spektraris family databases. 7. Molecular, biochemical and transcriptomic analyses on Arabidopsis mutants will be performed to show the impact of deleted domains on subcellular localization of the modified AtSR1/CAMTA3 proteins, the effect of calmodulin-binding to AtSR1/CAMTA3, and to determine their ability to regulate EDS1 expression. Whole-Genome Tilling will be used to detect the direct downstream targets of AtSR1/CAMTA3. 8. To test how the activated P2K receptor contributes to trigger defense responses functional analysis of the P2K receptor in defense responses against pathogens and insects will be performed complemented by pathogen infection assays. Co-immunoprecipitation experiments are planned to identify additional downstream components that comprise the P2K receptor complex, using LC-MS/MS confirmed by Bi-FC and yeast two-hybrid assay. Furthermore, for studying extracellular ATP signaling pathway, RNA-seq and phosphoproteomics analysis will be employed. New danger signals in pathogen-infected hairy root of potato plants will be identified by NMR or mass spectrometry. 9. Reverse genetic screens, gene editing, and mutagenized TILLING populations in Arabidopsis, camelina, canola, Brachypodium and rice will be employed to identify the roles of genes associated with BR-inactivation, seedling and adult development. Reverse genetics will include the use of transgenic plants as well as CRISPR/Cas9-based gene editing. By using a combination of forward genetics, molecular genetics, genomics, and traditional breeding new varieties of grasses (Kentucky bluegrass, the ancient grain teff, western wheatgrass, prairie june grass, tufted hairgrass, bread wheat) that can be used for agricultural and horticultural purposes will be identified. 10. The potato excised stem assay and pea endocarp tissue will be used to analyze the critical initial development and defense gene activation. 11. Peroxisome abundance in Kronos TILLING population will be determined in tissue extracts. The effect of peroxisome abundance on drought tolerance will be assessed in greenhouse conditions. Fitness will be measured as a grain number, total weight of grains per spike, and number of spikes. Functional conservation of identified genes will be examined the using reverse genetics in Arabidopsis and Brachypodium. 12. Methods for targeted transfer of genes from wild relatives into crops without the 'linkage drag' of unwanted chromatin will be developed. This will be done by transient silencing of the C-Ph1 gene by virus induced gene silencing or RNAi. 13. Molecular, genetic, physiological, biochemical, and -omics-level approaches will be used for comparative studies of the BUZZ kinase. To determine the target proteins of the putative BUZZ kinase, we will use comparative phosphoproteomics to compare wild-type BUZZ and the buzz mutant complemented by co-immunoprecipitation (co-IP) assays. EMS mutagenized lines will be screened to identify buzz suppressor loci using high through put phenomics. 14. Bacterial genetics will be used to probe the symbiotic state of nitrogen and carbon metabolism and to monitor the activity of bacterial genes during symbiosis. A unique nitrogen-fixing but ineffective bacterial mutant will be employed that is affected in a stress response regulatory gene and involves a plant defense mechanism. 15. Gene replacement strategies will be employed to place defined modification into the two-component regulatory gene system nifL and nifA in A. vinelandii. Diazotrophic growth phenotype, patterns of gene expression, and ammonia production in mutant strains with defined deletions in regions of nifL and nifA will be used to delineate regulatory control factors. 16. Root-knot nematode effectors will be used as molecular probes in yeast two hybrid assays to find their potato interaction partners. By obtaining information about nematode secretions and the plant proteins they target in the host we will identify plant processes that will be the focus for potato resistance engineering.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:The main audience is the scientific community working on the different aspect of plant biology covered by this MPS Hatch umbrella. Knowledge gained by this consortium will help solve pressing agricultural problems that are relevant for Washington State growers as well as farmers in other states and countries worldwide. Several members of the consortium actively collaborate with the local commodity commissions, farmers and other stakeholders. These interactions will be used to communicate findings of the proposal to stakeholders. Members of general public were informed about outcomes of our work through press releases, publications in magazines, and podcasts. Changes/Problems:The progress during 2020 was hindered by COVID-19 pandemic. As the disease was spreading, the Washington State University as well as Washington State Government took measures to minimize the risk to the employees. The university was closed from the Middle of March until the beginning of May. During the remaining period laboratory activities were restricted by the extreme social distancing measures. As a consequence, research activities were significantly slowed down. Another problem was associated with cancellation of conferences. Both University undergraduate research show and the Annual Meeting of the American Society of Plant Biologists were cancelled or moved to virtual platform, which complicated dissemination of the knowledge and getting feedback on our results. However, the MPS Hatch umbrella group was able to conduct its annual meeting virtually via zoom on October 14, 2020. What opportunities for training and professional development has the project provided?The project provided training to PostDocs, PhD students, M.S. students, and undergraduate students that totalsto83students and PostDocs as summarized in the following table. The training included practicing and improving different scientific techniques and presenting results and data on research conferences, meetings, workshops, and symposia. How have the results been disseminated to communities of interest?The main venue for dissemination of products of the MPS Hatch umbrella project is publication as journal articles and presentations at national and international conferences and meetings. The long list of 58 published papers in peer-reviewed scientific journals complemented by one book chapters, three publications in the stakeholder-centered magazines and the high number of invited oral presentations emphasizes the excellent scholarly achievement of this group. This is complemented by three Ph.D. dissertations during the reporting period. Furthermore, presentations were given to stakeholders and farmers. Finally, research from this Hatch umbrella groupresults into two patents. What do you plan to do during the next reporting period to accomplish the goals?Dr. Bates:Continue to investigate the control of fatty acid flux into membrane lipids vs oils in seed and biomass leaf tissues. Dr. Browse: Continue to investigate the potential for castor enzymes to produce hydroxyl fatty acids in transgenic Arabidopsis. Dr. Gang: Further characterization of enzymes in biochemical pathways will be performed by the new graduate student. Hemp chemical composition will be evaluated based on genotype by environment interactions. Dr. Gill:Continue to understand mode of action of the Ph1 gene. Specifically, mutants for the gene will be identified to differentiate function of the 5B copy from that of the other two copies. Dr. Gleason: Continue to identify key root-knot nematode effectors and their plant targets in Arabidopsis and potato. Dr. Hadwiger:To continue peptide research this time to look at cytology of the resistance induced by peptides observed as fungal growth inhibition by microscope. Dr. Kahn:Establish the mechanism of three genes shown to improve certain symbiotic interactions. Develop techniques to test the potential for symbiotic nitrogen fixation in space. Dr. Kirchhoff: Further unraveling architectural dynamics in thylakoid membranes with special focus on the sub-localization of the cytochrome b6f complex and on establishing computer models of thylakoid membranes. Dr. Lange:Roll out spectral databases for taxanes of the genus Taxus (> 600 metabolites) and cannabinoids of the genus Cannabis (> 200 metabolites). Dr. Neff: Continue to study the role of BR-inactivating genes, and their transcriptional regulation, with regard to seedling and adult plant development. Also continue to describe the role of dominant-negative alleles inAHLgenes with regard to regulating seed size and seedling development. Continue to clone and characterize VRN-related genes in Kentucky Bluegrass. Finally, continue to work with WSU to build a new Native Grass Ecology and Breeding Farm to facilitate breeding new varieties of turfgrasses as well as other grasses native to North America. Dr.Okita: Complete studies on the changes of the transcriptome during rice grain development.Generate specific DNA segmental deletions of the L80 coding region. Dr.Peters:Focus on tailoring strains of ammonia excretingA. vinelandiito promote colonization of specific crop plants by altering the carbon metabolism to be dependent on specific exudate compounds. Model the carbon cost of ammonia excretion as a function of specific exudate compounds. Dr.Sanguinet: Validate and determine additional BUZZ protein-protein interactions and generate stable transgenic lines to determine subcellular and tissue localization. Dr.Smertenko: Produce antibody for peroxisome proliferation markers in wheat, PEX11 and catalase; analyze peroxisome proliferation in response to combination of heat and drought; identify markers of more efficient ROS scavenging in biparental population between two spring wheat varieties Kelse x Scarlet. Isolate transcription factors that bind promoters of peroxisome biogenesis genes. Dr.Tanaka:Further investigate the downstream signaling following extracellular ATP recognition. We are currently looking at the signaling pathway in bryophytes. Additionally, We continuously try for identification of novel elicitors from infected potato tissues.

Impacts
What was accomplished under these goals? The project is divided into 6 research areas: A1-A3 (Products and Synthesis) and B1-B3 (Defense and Signaling). Accomplishments in these areas are summarized below. A1 Primary metabolism (Kirchhoff, Okita) Dr. Kirchhoff:An optimized method for incorporation of an isolated membrane protein into large unilamellar lipid vesicles revealed how non-bilayer lipids modulates photosynthetic light harvesting efficiency. Furthermore, a new mutant study identified plastocyanin as long-range electron carrier in photosynthetic electron transport in plants. Finally, the Kirchhoff lab optimized methods for leaf sample preparations for TEM.. Dr.Okita: The interaction of Pho1 with PsaC and PsaD was confirmed further supporting that the starch enzyme interact with photosystem I.The expressionof the Pho1 gene in rice was eliminated by CRISPR technology. The Pho1 knock-out rice lines showed growth phenotype. Transgenicpho1-rice mutant lines expressing a Pho1 lacking the L80 sequences grew poorly when subjected to suboptimal 20°C. Further studies support the role of membrane trafficking in the transport and localization of plant mRNAs to their intracellular destination sites. A2 Secondary metabolism (Bates, Browse, Roje, Lewis, Gang, Lange) Dr.Bates: We demonstrated that the major seed oil producing enzymes DGAT1, and DGAT2 or PDAT1 utilize kinetically distinct substrate pools, affecting seed oil fatty acid composition. In addition, we analyzed mechanisms of acyl flux in oil-producing tobacco leaves and found that high flux of fatty acids through the membrane lipid PC contribute to the accumulation of polyunsaturated fatty acids. Dr.Browse: We showed that transgenic expression of three castor enzymes work in concert to produce the highest reported level of hydroxyl fatty acids produced in Arabidopsis. The cooperative nature of these expressed acyltransferases reveals their selectivity with respect to lipid precursors in the pathway. In addition, the efficient incorporation of hydroxy fatty acids led to oil levels similar to that in wild-type Arabidopsis. Dr.Gang: We demonstrated that the pathogen causing citrus greening disease, "CandidatusLiberibacter asiaticus", lives in biofilm communities where it interacts with other bacterial species that affect its ability to grow. We demonstrated that important specialized compounds in plant tissues are often not measured accurately and provided solutions to circumvent this problem. Dr. Lewis:Development of genetically modified Chlorella sorokiniana capable of biosynthesizing phenylethanol and/or phenylethanol glucoside (PEA-Glc) was achieved. Analysis of the metabolites produced by transgenicC. sorokinianalineCs1412 confirmed thatPEA-(Glc) production was successfully attained in transgenic Cs colonies that were expressing the three target GOIs, phenylacetaldehyde synthase (CsPAAS), phenylacetaldehyde reductase (CsPAR) and UDP-glucosyltransferase (CsUGT). A3 Roots / Products and Synthesis (Sanguinet, Kahn) Dr.Sanguinet: We were able to express recombinant BUZZ protein, but could not detect any kinase activity--neither trans or autophosphorylation. We performed a yeast two-hybrid screen and identified 4 high-confidence protein-protein interactors and another 25 moderate-confidence interactors. We are working on BiFC assays and pull-down assays to confirm the interactions. B1 Signals and Hormones (Poovaiah, Tanaka, Neff) Dr.Neff: We demonstrated that CRISPR-Cas9 can be used to impact begomovirus infection in plants. We addressed the role of photomorphogenesis in disease resistance in plants. We also demonstrated that AHL and NAC-family transcription factors regulate photomorphogenesis in plants. We also demonstrated that a particular mutation in the AHLSOB3can increase seed size and seedling emergence in plants. We elucidated an emerging problem with a particular gelling agent when studying photomorphogenesis in seedlings. Finally, we have now fully established our new Grass Breeding and Ecology Farm at WSU. Dr.Tanaka:A new signaling caused by DAMPs were discovered and several molecular components were identified. We also discovered a new DAMP elicitor that enhances plant resistance against a broad range of plant pathogens. Several methods to measure early defense responses against several pathogens in different plant species were also established. The methods will be useful to explore conserved plant immune responses among a variety of plants. B2 Stress Response (Hadwiger, Smertenko, Gill) Dr.Gill: By detailed expression analysis, we have shown that the Ph1 function evolved via changes in the expression pattern of one of the three gene copies such that the 5B copy of the gene started to specifically express during Interphase and Prophase I of meiosis. Dr.Hadwiger: We found that compounds that can induce a defense response can have basic charges that enable the elicitor to enter the cell and localize in the plant nucleus. Also peptides that can originate in the fungal cell when transferred to the plant host's nucleus can activate defense genes. The mechanism hypothesized to cause this defense gene activation can do so by altering the construction of chromatin in a manner that allows the transcription to progress through the defense gene codes. The new alterations by specific peptides and some of the anti-cancer drugs such as netropsin are hypothesized to target the AT-rich regions and the minor groove. Dr.Smertenko: We identified two consistent markers, Peroxin 11 and Catalase for peroxisome proliferation under drought stress in wheat. Furthermore, we demonstrated that microtubule-binding protein MAP20 localizes to the borders of pits in xylem cells during later stages of vascular bundles differentiation. Knock-down of MAP20 results in larger pits with thinner pit membrane. The mutants have deficiency in recovery from the drought stress. These results mean that architecture of vascular pits defined by MAP20 is essential for adaptation to drought. B3 Roots / Defense and Signaling (Peters, Gleason) Dr.Gleason: We discovered that a plant peptide can be applied to potato roots to stimulate plant resistance against root-knot nematodes. We have developed molecular beacon assays for the identification of important potato-infecting root-knot nematodes. Dr.Peters: Refined the understanding of the genetic determinations of ammonia excretion phenotype inAzotobacter vinelandiiand generated strains through gene editing that excrete different amounts of ammonia in cell culture. The transfer of ammonia from engineered strains to several crop plants was demonstrated. We have developed and implement metabolic modeling of nitrogen fixation inA. vinelandiiunder different environmental growth conditions and during ammonia excretion allowing estimation of the carbon cost of ammonia excretion.

Publications

  • Type: Other Status: Published Year Published: 2020 Citation: Brew-Appiah RAT, Sanguinet KA (2020) Analysis of SALP1 genes in wheat for stress tolerance, Lind Field Day Abstract, June 2020
  • Type: Other Status: Published Year Published: 2020 Citation: Peracchi LP, Brew-Appiah RAT, Sanguinet KA (2020) Exploring CAD genes as agents for stress-tolerant wheat, Lind Field Day Abstract, June 2020
  • Type: Book Chapters Status: Published Year Published: 2019 Citation: Ramachandran SR, Kumar S, Tanaka K (2019) Quantification of extracellular ATP in plant suspension cell cultures. In Plant Innate Immunity: Methods and Protocols (W. Gassmann, ed.). Methods Mol. Biol., Vol 1991, pp. 43-54 (Chapter 5). Humana, New York, NY.
  • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: Grant, N (2020) Exploring the natural variation of photosynthesis and abiotic stress in wheat varieties and reduced height mutants. Ph.D. thesis. Molecular Plant Sciences. Washington State University.
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Ma, Xiaochi (2019) Physiological and Molecular Responses of Cabernet Sauvignon (Vitis vinifera L.) to Novel Water Management Strategies. PhD Thesis. Crop Science. Washington State University.
  • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: Oung, HMO (2020) The protection strategies realized in a resurrection plant. Ph.D. thesis. Molecular Plant Sciences. Washington State University
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Bates: 8th Asian-Oceanian Symposium on Plant Lipids. Canberra, Australia.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Browse: VIB Conference on Regulatory Oxylipins. University of Ghent, Belgium. Sex and the Single Hormone. April 1  4, 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gleason: Plant Pathology Department Seminar, UC Riverside; Department of Crops and Soils Seminar, Washington State University; Washington-Oregon Potato Conference (Kennewick, WA).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Kirchhoff: Tegernsee (Germany) DFG-FOR2092 international conference: More than 60 years of photosynthesis research: How do PSII and PSI functionally connect in higher plants (November 2019)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Kirchhoff: Maria an der Aue (Germany) CEPLAS summer school: Dynamic architecture of photosynthetic membranes. (May 2019)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Kirchhoff: Baltimore (MD, USA) Biophysical Society-Bioenergetics Subgroup: Quinone diffusion in photosynthetic membranes: Challenges caused by complex membrane architectures. (March 2019)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Dr. Lange: Invited presentations at the Sixth International Conference on Drug Discovery, Development and Lead Optimization scheduled during. Untangeling the Mystery of the Entourage Phytochemicals in Cannabis, October 5-7, 2020; Virtual Meeting on Medicinal Development of Cannabis and Cannabinoids Evolution of Cannabinoid Diversity in Cannabis  What Have We Learned from Genomes, Genomics, and Biochemistry, August 11, 2020; Emerald Conference Inseparable Twins?  The Biochemistry Underlying Cannabinoid and Terpenoid Biosynthesis, February 26-28, 2020
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: Washington Turfgrass Seed Commission annual meeting (Pullman WA); Spokane Ag Show/Pacific Northwest Farm Forum (Spokane WA) (cancelled due to weather); 23rd Annual Noxious Weeds Conference (Moses Lake WA).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Okita: Kyushu University, Institute of Genetics, October 31, 2019 (Interaction of Pho1 with photosystem I) and November 1, 2019 (Role of RNA localization in plants).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Dr. Sanguinet: The role of the BUZZ kinase in post-initiation root hair growth. 28th Plant and Animal Genome Meeting, San Diego, CA January 14, 2020.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Smertenko: Pacific Northwest National Laboratory, Department of Energy, Richland, WA;State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Karki N, Johnson BS, Bates PD (2019) Metabolically Distinct Pools of Phosphatidylcholine Are Involved in Trafficking of Fatty Acids out of and into the Chloroplast for Membrane Production. Plant Cell 31: 2768-2788.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kotapati HK, Bates PD (2020) Normal phase HPLC method for combined separation of both polar and neutral lipid classes with application to lipid metabolic flux. Journal of Chromatography B 1145: 122099.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Zhou XR, Bhandari S, Johnson BS, Kotapati HK, Allen DK, Vanhercke T, Bates PD (2020) Reorganization of Acyl Flux through the Lipid Metabolic Network in Oil-Accumulating Tobacco Leaves. Plant Physiol 182: 739-755. NIFA Support Acknowledged: Yes.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Regmi A, Shockey J, Kotapati HK, Bates PD (2020) Oil-Producing Metabolons Containing DGAT1 Use Separate Substrate Pools from those Containing DGAT2 or PDAT. Plant Physiology 184: 720-737.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lunn, D., Wallis, J. G. and Browse, J. 2019 Tri-Hydroxy-Triacylglycerol is Efficiently Produced by Position Specific Castor Acyltransferases. Plant Physiol. 179: 1050-1063.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Bai S, Engelen S, Denolf P, Wallis JG, Lynch K, Bengtsson JD, Van Thournout M, Haesendonckx B, Browse J. (2019) Identification, Characterization and Field Testing of Brassica napus Mutants Producing High-Oleic Oils. Plant J. 98: 33-41.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Bai S, Engelen S, Denolf P, Wallis JG, Lynch K, Bengtsson JD, Van Thournout M, Haesendonckx B, Browse J. 2020 The biochemistry of headgroup exchange during triacylglycerol synthesis in canola. Plant J. 103: 83-94.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ruhnaz Kashfi, Charles Kelsey, David Jorgen Gang, Douglas R. Call, David R Gang. 2020. Metabolomic diversity and identification of antibacterial activities of bacteria isolated from marine sediments from Hawaii and Puerto Rico. Frontiers in Metabolomics. 7:23.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: KJ Brownstein, S Tushingham, WJ Damitio, T Nguyen, DR Gang. 2020. An ancient residue metabolomics-based method to distinguish use of closely related plant species in ancient pipes. Frontiers in Molecular Biosciences 7:133.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Karol L Krey, Paul D Nabity, Carmen K Blubaugh, Zhen Fu, James T Van Leuven, John P Reganold, Anna Berim, David R Gang, Andrew S Jensen, William E Snyder. 2020. Organic farming sharpens plant defenses in the field. Frontiers in Sustainable Food Systems 4:97.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Anna Berim, David R Gang. 2020. Extractability, stability, and accumulation of nepetoidins in Ocimum basilicum L. leaves and cell cultures. Plant Cell, Tissue and Organ Culture (PCTOC) 143(1):75-85.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Banafsheh Molki, Douglas R Call, Phuc T Ha, Anders Omsland, David R Gang, Stephen R Lindemann, Nabil Killiny, Haluk Beyenal. 2020. Growth of Candidatus Liberibacter asiaticus in a host-free microbial culture is associated with microbial community composition. Enzyme and Microbial Technology 142:109691.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2020 Citation: Bheemanahalli Raju, Somayanda Impa, Krassovskaya Inga, Vennapusa Amaranatha; Gill Kulvinder, Obata Toshihiro, Jagadish Krishna S V (2020) Enhanced N-metabolites, ABA and IAA-conjugate in anthers instigate heat sensitivity in spring wheat. Physiologia Plantarum
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Gill Kulvinder, Kumar N, Randhawa HS, Carter AH, Morris CF, Baik BK, Higginbotham RW, Engle DA, Guy SO, Burke I, Lyon D, Murray TD, Chen XM (2020) Registration of Curiosity CL+ Soft White Winter Wheat. Journal of Plant Registrations 14 (3): 377-387.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Gill, Kulvinder, Kumar N, Randhawa HS, Carter AH, Yenish J, Morris CF, Baik BK, Higginbotham R W, Guy SO, Engle DA, Burke I, Chen XM, Murray TD, Lyon D (2020) Registration of Mela CL+ Soft White Winter Wheat. Journal of Plant Registrations 14 (2) 144-152
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Erdayani E, Nagarajan R, Grant N, Gill K (2020) Identification and characterization of the CLPB/HSP100 gene family in bread wheat. Scientific Reports 10 (1), 1-17.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Mohan A, Dhaliwal AK, Nagarajan R, Gill K (2019) Molecular Characterization of Auxin Efflux Carrier- ABCB1 in hexaploid wheat. Scientific Reports, 9, 1-14.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Singh K, Khan MA, Gill K (2019) The novel function of the Ph1 gene to differentiate homologs from homoeologs evolved in Triticum turgidum ssp. dicoccoides via a dramatic meiosis-specific increase in the expression of the 5B copy of the C-Ph1 gene. Chromosoma, 128(4), 561-570.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Hinojosa L, Kumar N, Gill K, Murphy K (2019) Spectral Reflectance Indices and Physiological Parameters in Quinoa Under Different Irrigation Regimes. Crop Science, 59, 1927-1944.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Bheemanahalli R, Sumoj VJ, Saripalli G, Prasad PV, Balyan HS, Gupta PK, Gill KS, Jagadish SK (2019) Quantifying the impact of heat stress on pollen germination, seed-set and grain filling in spring wheat. Crop Science, 59(2):684-696.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Lei Zhang and Cynthia Gleason (2020) Enhancing potato resistance against root-knot nematodes using plant elicitors delivered by bacteria. Nature Plants 6: 625629.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Roy A, Zhai Y, Ortiz J, Neff MM, Mandal B, Mukherjee SK and Pappu HR (2019) Multiplexed editing of a begomovirus genome restricts escape mutant formation and disease development PLoS ONE 14: (10).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Peng H and Neff MM (2020) CCA1 and ATAF2 differentially suppress cytochrome P450-mediated brassinosteroid inactivation in Arabidopsis Journal of Experimental Botany 71 (3); 970-985.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Favero DS, Kawamura A, Shibata M, Takebayashi A, Jung J-H, Suzuki T, Jaeger KE, Ishida T, Iwase A, Wigge PA, Neff MM and Sugimoto K (2020) AT-hook transcription factors repress petiole growth by antagonizing PIFs. Current Biology 30, 1454-1466.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Jacques CN, Hulbert A, Westenskow S and Neff MM (2020) Production location of the gelling agent, Phytagel, has a significant impact on Arabidopsis thaliana seedling phenotypic analysis PLoS ONE 15(5): e0228515.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Zhai Y, Peng H, Neff MM and Pappu HR (2020) Emerging molecular links between plant photomorphogenesis and virus resistance. Frontiers in Plant Science 11:920.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Koirala PS and Neff MM (2020) Improving seed size and seedling emergence in transgenic Camelina sativa by overexpressing the Atsob3-6 gene variant. Transgenic Research 29:409-418.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Peng H, Phung J and Neff MM (2020) Self-transcriptional repression of the Arabidopsis NAC transcription factor ATAF2 and its genetic interaction with phytochrome A in modulating seedling photomorphogenesis. Planta 252(4):48.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Chou, H.-L., L. Tian, T. Kumamaru, and T.W. Okita. (2019) The role of RNA binding protein OsTudor-SN in post-transcriptional regulation of seed storage proteins and cell development in developing rice endosperm. Plant Cell Physiol. 60(10):2193-2205.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Tian, L., H.-L. Chou, M. Fukuda, T. Kumamaru, and T.W. Okita (2020) mRNA localization in plants. Plant Physiol. (Focus Issue on RNA Biology) 182:97-109.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Hwang, S.-K., K. Koper and T.W. Okita (2020) Plastidial phosphorylase as a multiple-role player in plant metabolism. Plant Sci. 290, 110303.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Tian, L. K. Doroshenk, L. Zhang, M. Fukuda, A. Nagamine, H. Washida, T. Kumamaru, and T.W. Okita (2020) Glutelin mRNAs are transported to the cortical-ER by the cooperative activity of zipcode RNA binding proteins with Rab5 and NSF in rice endosperm cells. Plant Cell 32:2566-2581.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: K.A. Brown, J. Ruzicka, H. Kallas, B. Chica, D.W. Mulder, J.W. Peters, L.C. Seefeldt, G. Dukovic, Paul W. King Excitation-rate determines product stoichiometry in photochemical ammonia production by CdS quantum dot-nitrogenase MoFe protein complexes ACS Catalysis 10:11147-11152 (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: N. Pence, N. Lewis, A.B. Alleman, L.C. Seefeldt, J. W. Peters A potential role for the gamma subunit in mediating interactions between the nitrogenases for efficient nitrogen fixation in Azotobacter vinelandii J. Inorg. Biochem. (2020).
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2020 Citation: A.B. Alleman, Y.A. Mohammed, K.A. McVay, Q.A. Khan, P. Carr, J. Miller, Z. Miller, J. Torrion, P. Lamb, F. Mus, C. Chen, and J.W Peters Drivers of diazotrophic bacterial community structure and co-occurrence in a Northern Great Plains Pulse Crop Rotation System Appl. Soil. Ecol.
  • Type: Book Chapters Status: Published Year Published: 2020 Citation: F. Varghese, O.A. Zadvornyy, J.W. Peters Flavin-based electron bifurcation In Encyclopedia of Biological Chemistry, 3rd Edition Elsevier Amsterdam, The Netherlands (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: J. L. Yuly, P. Zhang, C.E. Lubner, J.W. Peters, D.N. Beratan Universal free energy landscape produces efficient and reversible electron bifurcation Proc. Natl. Acad. Sci. USA 117:21045-21051 (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: F. Mus, H.H. Wu, A.B. Alleman, K.A. Shisler, O.A. Zadvornyy, B. Bothner, J.L. Dubois, J.W. Peters Insights into the unique carboxylation reactions in the metabolism of propylene and acetone Biochem. J. 477:2027-2038 (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: J.A. Laureanti, B. Ginovskaa, G.W. Buchko, G.K. Schenter, M. Hebert, O.A. Zadvornyy, J.W. Peters W.J. Shaw A positive charge in the outer coordination sphere of an artificial enzyme increases CO2 hydrogenation Organometallics 39: (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: J.H. Artz, O.A. Zadvornyy, D.W. Mulder, S.M. Keable, A.E. Cohen, M.W. Ratzloff, S.G. Williams , B. Ginovska, N. Kumar, J. Song, S.E. McPhillips, C.M. Davidson, A.Y. Lyubimov, N. Pence, G.J. Schut, A.K. Jones, S.M. Soltis, M.W.W. Adams, S. Raugei, P.W. King, J.W. Peters Tuning catalytic bias of hydrogen gas producing hydrogenases J. Am. Chem. Soc. 142:1227-1235. (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: M.-H. Ryu, J. Zhang, T. Toth, D. Khokhani, B.A. Geddes, F. Mus, A. Garcia-Costas, J.W. Peters, P.S. Poole, J.-M. An�, C.A. Voigt Control of nitrogen fixation in bacteria that associate with cereals Nat. Microbiol. 5:314-330 (2020).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Taylor SE, Pearce CI, Sanguinet KA, Hu H, Chrisler WB, Kim Y-M, Wang Z, Flury M. (2020) Polystyrene nano- and microplastic accumulation at arabidopsis and wheat root cap cells, but no evidence for uptake into roots, Environmental Science: Nano 7.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ma X, Jacoby P, Sanguinet KA. (2020) Improving net photosynthetic rate and rooting depth of grapevines through a novel irrigation strategy in a semi-arid climate. Frontiers in Plant Science 11, 1137.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ghimire B, Hulbert S, Steber C, Garland-Campbell K, Sanguinet KA. (2020) Characterization of root traits for improvement of spring wheat in the Pacific Northwest, Agronomy Journal, 121: 1(228-40)
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ma X, Sanguinet KA, Jacoby P. (2020) Direct root-zone irrigation outperforms surface drip irrigation for grape yield and crop water use efficiency while restricting root growth. Agricultural Water Management, 231, 105993
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Cong W, Miao Y, Xu L, Zhang Y, Yuan C, Wang J, Zhuang T, Lin X, Jiang L, Wang N, Ma J, Sanguinet KA, Liu B, Rustgi S, Ou X. (2019) Transgenerational memory of gene expression changes induced by heavy metal stress in rice (Oryza sativa L.) BMC Plant Biology 19 (1): 282
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Smertenko T, Turner G, Fahy D, Brew-Appiah RAT, Alfero-Alco R, de Almieda Engler J, Sanguinet KA, Smertenko A. (2020) Brachypodium distachyon MAP20 functions in metaxylem pit development and contributes to drought recovery, New Phytologist, 227 (6): 1681-1695
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: C. Bryant, J. Ruzicka, H. Kallas, D.W. Mulder, K.A. Brown, J.W. Peters, L.C. Seefeldt, G. Dukovic, .W. King Defining intermediates of Nitrogenase MoFe protein during N2 reduction under photochemical electron delivery from CdS quantum dots J. Am. Chem. Soc. 142:14324-14330 (2020).
  • Type: Journal Articles Status: Accepted Year Published: 2020 Citation: Smertenko A., Clare S.J., Effertz K., Parish A., Ross A., and Schmidt S. (2020) A guide to plant TPX2-like and WAVE-DAMPENED2-like proteins. Journal of Experimental Botany
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Minina E. A., Staal J., Alvarez V. E., Berges J. A., Berman-Frank I., Beyaert R., Bidle K. D., Bornancin F., Casanova M., Cazzulo J. J., Choi C. J., Coll N. S., Dixit V. M., Dolinar M., Fasel N., Funk C., Gallois P., Gevaert K., Gutierrez-Beltran E., Hailfinger S., Klemencic M., Koonin E. V., Krappmann D., Linusson A., Machado M. F. M., Madeo F., Megeney L. A., Moschou P. N., Mottram J. C., Nystrom T., Osiewacz H. D., Overall C. M., Pandey K. C., Ruland J., Salvesen G. S., Shi Y. G., Smertenko A., Stael S., Stahlberg J., Suarez M. F., Thome M., Tuominen H., Van Breusegem F., van der Hoorn R. A. L., Vardi A., Zhivotovsky B., Lam E., and Bozhkov P. V. (2020) Classification and Nomenclature of Metacaspases and Paracaspases: No More Confusion with Caspases. Molecular Cell 77, 927-929.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kumar S, Tripathi D, Okubara PA, Tanaka K (2020) Purinoceptor P2K1/DORN1 enhances plant resistance against a soilborne fungal pathogen, Rhizoctonia solani. Front. Plant Sci.11: 572920.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Moroz N, Tanaka K (2020) FlgII-28 is a major flagellin-derived defense elicitor for potato. Mol. Plant-Microbe Int. 33: 247-255.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: DeShields JB, Moroz N, Braley LE, Mora-Romero GA, Tanaka K (2019) Recombinase polymerase amplification for the rapid isothermal detection of Spongospora subterranea f. sp. subterranea and potato mop-top virus. Am. J. Potato Res. 96: 617-624.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: M. Li, R. Mukhopadhyay, V. Svoboda, H.M.O. Oung, D.L. Mullendore, H. Kirchhoff (2020) Measuring the dynamic response of the thylakoid architecture in plant leaves by electron microscopy. Plant Direct 4, e00280.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: R. H�hner, M. Pribil, M. Herbstov�, L.S. Lopez, H.-H. Kunz, M. Li, M. Wood, V. Svoboda, S. Puthiyaveetil, D. Leister, H. Kirchhoff (2020) Plastocyanin is the long-range electron carrier between photosystem II and photosystem I in plants. Proc. Natl. Acad. Sci. USA 117, 15354-15362.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: S Tietz, M Leuenberger, R H�hner, AH Olson, GR Fleming, H Kirchhoff (2020) A proteoliposome-based system reveals how lipids control photosynthetic light harvesting. J. Biol. Chem. 295, 1857-1866.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: D. Schneider, L.S. Lopez, M. Li, J.D. Crawford, H. Kirchhoff, H.H. Kunz (2019) Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM. Plant Methods 15, 156.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: D.A. Gacek, C.-P. Holleboom, S. Tietz, H. Kirchhoff, P.J. Walla (2019) The role of the PsbS protein for carotenoid-chlorophyll coupling in grana thylakoids. FEBS Letters 593, 3190-3197.
  • Type: Other Status: Published Year Published: 2020 Citation: Lei Zhang and Cynthia Gleason (2020) Loop-Mediated Isothermal Amplification for the Diagnostic Detection of Meloidogyne chitwoodi, Potato Progress Vol XX, No. 1.
  • Type: Other Status: Published Year Published: 2020 Citation: Cynthia Gleason and Sagar Sathuvalli (2020) Genetic Diversity in Columbia Root-Knot Nematode, and a Request for Help in Research. Potato Progress Vol XX, No. 13
  • Type: Other Status: Published Year Published: 2020 Citation: Smertenko A. and Reznik E. (2020) Dealing with oxidation: new path towards wheat plant health revealed. Wheat Life 63 (7), 44-45.


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:The main audience is the scientific community working on the different aspect of plant biology covered by this MPS Hatch umbrella. Knowledge gained by this consortium will help solve pressing agricultural problems which are relevant for Washington growers as well as farmers worldwide. Several members of the consortium actively collaborate with the Washington crop Commissions, farmers and other stakeholders. These interactions will be used to communicate findings of the proposal to stakeholders. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project provided training to PostDocs, PhD students, M.S. students, undergraduate students and high school students that totalsto108students and PostDocs as summarized in the following table. The training included practicing and improving different scientific techniques and presenting results and data on research conferences, meetings, workshops, and symposia. PostDocPhD studentsMS studentsundergraduatesHigh school students Bates2 33 Browse 12 Gang1 1 Gill 3 53 Gleason2 1 23 Kahn1 3 Kirchhoff 22 Lange 11 Lewis Neff 1 5281 Okita2 31 Peters3 311 Pooviah 1 Sanguiet1 4 141 Roje 1 Smertenko3 36 Tanaka3 2131 TOTAL22 36 6404 How have the results been disseminated to communities of interest?The main venue for dissemination of products of the MPS Hatch umbrella project is publication as journal articles and presentations at national and international conferences and meetings. The long list of 56 published papers in peer-reviewed scientific journals complemented by two book chapters, and the high number of invited oral presentations together with the organization of international and national meetings emphasizes the excellent scholarly achievement of this group. This is complemented by completing four Ph.D. dissertations and three Master theses during the reporting period. Furthermore, presentations were given to stakeholders and farmers. Finally, research from this Hatch umbrella group results into four patents. What do you plan to do during the next reporting period to accomplish the goals?All project participants will continue and develop their projects as described in the narrative of the proposal. Some specific activities are listed below: Bates:Continue to investigate the control of fatty acid flux into membrane lipids vs oils in seed and biomass leaf tissues. Browse: Continue to investigate the potential for fatty acid desaturases in producing improved oils. Gang: Effort this year will focus on the metabolic interactions between plants and their pests. Gill: Continue to understand mechanism of chromosome pairing control and to use the C-Ph1 gene in inducing alien introgression. Gleason:Continue to identify key root-knot nematode effectors and their plant targets in Arabidopsis and potato. Hadwiger: Design experiments on both potato and pea to test whether suppressed DNA damage repair extend the period that plant defenses are present. Kahn:Finish work with PCO and write manuscripts. Kirchhoff: Determine light-induced dynamics of the sub-localization of the cytochrome b6f complex and its functional consequences.Publishing paper on the role of non-bilayer lipids on light harvesting. Refine electron microscopic-based quantitative analysis on intact leaf tissues. Lange:Continue to expand the NMR spectral coverage of natural products in the Spektraris database.Continue to support colleagues with structural identification of metabolites with roles in plant fitness and human nutrition. Neff: Continue to study the role of BR-inactivating genes, and their transcriptional regulation, with regard to seedling and adult plant development. Also continue to describe the role of dominant-negative alleles inAHLgenes with regard to regulating seed size and seedling development. Continue to clone and characterize VRN-related genes in Kentucky Bluegrass. Finally, begin performing research at WSUs new Perennial Grass Breeding and Ecology Farm Research Site to facilitate breeding new varieties of grasses native to North America. Okita: Complete studies on the role of the membrane trafficking proteins and RNA binding proteins in mediating RNA transport. Determine the impact of Pho1 and Pho1deltaL80 on photosystem I activity when temperature and reducing power is varied. Quantify PS1 complexes by quantitative immunoblotting using anti-PsaC and PsaB antibodies. membranes. Quantify PS1 complexes by quantitative immunoblotting using anti-PsaC and PsaB antibodies. Peters: Continue work on nitrogenase regulation and defining the determinants of ammonia excretion and the plant growth promoting properties ofAzotobacter vinelandiimutant strains. Refine our metabolic model for remodeling energy metabolism in diazotrophy forAzotobacter vinelandii. Pooviah: Continue studies on the mechanistic aspects of transcription factor (AtSR1) and its role in plant immune/defense response. Specifically, (1) continue to evaluate fluctuations in intracellular calcium concentrations/calcium imaging in response to microbial signals and study how these signals are amplified and produce the final responses; (2) continue studies on NPR1:AtSR1 interactions and study the role in immune response; and (3) explore the primary regulon of AtSR1. Sanguinet: Determine BUZZ-protein interactions. Publish work on BdBUZZ, AtBUZZ and BdCAMTA4. Continue to work with A. thaliana, B. distachyon, and wheat root development. Roje:Test biochemical function of a candidate for enzyme that catalyzes the first committed step in the biosynthesis of riboflavin in S. meliloti. Screen progeny of five Arabidopsis heterozygous knockout lines for adenylosuccinate synthase for homozygosity, and if found analyze the knockouts for changes in flavin metabolism. Smertenko: Measure expression of genes that control peroxisome proliferation and autophagy during drought stress in spring wheat. Prepare antibodies against wheat PEX11 and ATG8. Analyze impact of heat and drought stress of peroxisome proliferation and autophagy under field conditions. Tanaka: Further investigate the downstream signaling following extracellular ATP recognition by its receptor P2K1. Working on phospho-proteomics and interactomics for the DAMP receptor P2K1. Continuously try for identification of novel elicitors from infected potato tissues. We'll also work on the potential use of DAMPs as a novel class of plant protective bio-pesticides, using non-food transgenic delivery systems, which could be deployed on farmer-preferred cultivars.

Impacts
What was accomplished under these goals? A1 Primary metabolism (Kirchhoff, Okita) A new thylakoid membrane fractionation protocol was established and published that allows quantification of protein and lipid compositions in stacked unstacked thylakoid domains, respectively. This pave way for qualitative studies on protein redistribution dynamics in thylakoid membranes and for coarse grain computer modeling. In a further published paper it was shown that the protein density in stacked thylakoid areas plays a crucial role in photosynthetic light-harvesting and regulation. Studies on the role of OsTudor-SN in protein body formation and seed development as well as on the role of RBP-L in storage protein RNA localization were completed and published. In addition, experiments to show that the membrane trafficking factor, NSF, is closely associated with RBP-P biochemically and cytologically in developing rice endosperm were completed. Gas exchange and physiological studies of wild-type, pho1 mutant line, and transgenic pho1 lines expressing wild-type and Pho1ΔL80 were completed. Direct biochemical evidence that Pho1 is present on PS1 complexes isolated from thylakoid was obtained. A2 Secondary metabolism (Bates, Browse, Roje, Lewis, Gang, Lange) It was demonstrated that the LPCAT enzymes are essential for the direct incorporation of newly synthesized fatty acids into the membrane lipid PC in leaves and oil accumulation tissues. This incorporation into PC prior to accumulation in oil leads to high levels of polyunsaturated fatty acids in plant oils. Furthermore, expression of a 16:0 desaturase in the model oilseed, Camelina sativa resulted in a 30% reduction in saturate content of the oil. These experiments demonstrate that targeted heterologous expression of a desaturase in oilseeds can reduce the level of saturated fatty acids in the oil, significantly improving its nutritional value.In experiments with both poplar trees and the algae Chlorella sorokiniana it was demonstrated that the two species were successfully genetically modified to produce phenyl ethanol glucoside. Studies are in progress to optimize the levels on this secondary metabolite. A manuscript on this topic is in preparation. The biochemical characterization of the enzyme formamidase fromSinorhizobium melilotiinvolved in flavin biosynthesis was completed. A manuscript is in preparation. The analysis of RNAseq data generated with plants with different expression of two flavin biosynthesis enzymes reveal that the expression of these genes is linked to iron metabolism. This leads to the hypothesis that flavin metabolism is involved in distribution of iron in plant tissues. A3 Roots / Products and Synthesis (Sanguinet, Kahn) Wildtype and mutanted versions of the cell division kinases BUZZ were successfully over-expressed and purified for down-stream biochemical applications. Initial biochemical characterization of the over-expressed BUZZ were done. However, the proteins shown no in vitro activities. Stable transformants fused with GFP (on N or C terminus) will be generated. These findings were presented at an international meeting. Furthermore a PhD student was graduated and a new PhD student recruited to work on this project. In another project it was determined thatSinorhizobium meliloti1021 cannot grow on PCO as a carbon or nitrogen source. It was shown that iseA and other genes fromS. mediceaecan improve symbiosis of S. meliloti 1021 withMedicago truncatula. B1 Signals and Hormones (Poovaiah, Tanaka, Neff) The mutual interplay of calcium and ROS signaling in plant's immune system was discussed. Water stress and stomatal development were investigated.The findings revealed that allosteric control of the GTL1 transcription factor by calcium/calmodulin transduces water-deficit stress to the repression of stomatal development during drought stress acclimation. In another on flavonoid biosynthesis it was observed that enhanced salt tolerance of soybean inoculated with rhizobia is correlated with the decreased phosphorylatin of GmMYB183. Anew signaling crosstalk based on phenotypic and transcriptomic data between extracellular ATP was identified. It was discovered that the DAMP elicitors enhance plant resistance against a broad range of plant pathogens. Thissuggesting that signals determine plant defense response can improve the current crop protection from various pathogens and pests.Several methods to measure early defense responses against several pathogens in different plant specieswereestablished. The methods will be useful to explore conserved plant immune responses among a variety of plants. Studies onrepressors of the brasserosteroid (BR)-inactivating genesleads to discovery ofatranscription factor that isinvolved inregulating seedling development. In addition,a BR-inactivating quadruple mutant was phenotyped. It has beendemonstrated that thesob3-6allele ofSOB3is the only dominant-negative mutation that leads to increased seed size.It was discovered that theSOFL gene family inArabidopsisis larger than previously expected. Furthermore, vernalization genes in Kentucky bluegrass have been identified. Finally, planning has been started with WSU to design a new Native Grass Ecology and Breeding Farm. B2 Stress Response (Hadwiger, Smertenko, Gill) Fungal diseases of plants, like those that infect animals, are continuing to overcome fungicides. Therefore the development of other forms of disease resistance including the development of defense response-inducing agents must be understood and manipulated. Homologs of the peptide pep13 were developed. It was found that two of these homologs are superior to Pep13 in inducing phytoalexin production in pea endocarp tissue. The peptides are also capable of causing DNA damage which is a vital part of the activation of plant defense genes. A manuscript is in preparation. In the chromosome pairing project detailed structural and expression analysis of the Ph1 gene among wheat progenitors were done. It was shown that the chromosome pairing regulation is controlled by meiosis specific expression of the B copy of the gene. The 5B copy of the gene produces three alternate splice variants and their relative accumulation is dramatically different among various meiotic stages. Transiently silencing the gene induces recombination between wheat and its relative chromosomes. This process will be tremendous value in crop improvement for transferring value added genes from wild relatives into crops. In another project on wheat, a program was initiated that aimed at measuring peroxisome abundance in spring wheat varieties under field conditions. In 2019, 14 varieties were measured in Spillman farm near Pullman (annual rainfall about 20-22"), Lind (annual rainfall 10-12"), and in Moses Lake (irrigation). It is expected to identify varieties with contrasting performance under drought and irrigated conditions and to determine correlation between peroxisome abundance and yield. B3 Roots / Defense and Signaling (Peters, Gleason) The genetic determinants of ammonia excretion inAzotobacter vinelandiiwere discovered and its impact on plant growth promotion and incorporation of ammonia in crops plants were examined. A first generation metabolic model was developed that describes how energy metabolism is remodeled inAzotobacter vinelandiito support optimal diazotrophic growth. In the root-knot nematode project, potato plants were treated with the plant defense elicitor peptide StPep1, either with the commercially synthesized peptide or withBacillus subtilisdesigned to secrete the plant peptide StPep1. The objective was to enhance resistance in potato against the Columbia root-knot nematode (Meloidogyne chitwoodi). It was found that StPep1 primed potato roots for resistance against root-knot nematodes. It was further discovered thatBacilluscould be used as a non-food transgenic delivery system of plant defense peptides. A key outcome of this work was a provisional patent application.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Tanaka: Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology, Tokyo, Japan. July 31, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Tanaka: Biology of vector-borne disease workshop at University of Idaho. June 24, 2019
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Shockey J, Lager I, Stymne S, Kotapati HK, Sheffield J, Mason C, Bates PD (2019) Specialized lysophosphatidic acid acyltransferases contribute to unusual fatty acid accumulation in exotic Euphorbiaceae seed oils. Planta 249: 1285-1299.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Islam N, Bates PD, Maria John KM, Krishnan HB, Z JZ, Luthria DL, Natarajan SS (2019) Quantitative Proteomic Analysis of Low Linolenic Acid Transgenic Soybean Reveals Perturbations of Fatty Acid Metabolic Pathways. Proteomics 19: e1800379.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Karki N, Johnson BS, Bates PD (2019) Metabolically distinct pools of phosphatidylcholine are involved in trafficking of fatty acids out of and into the chloroplast for membrane production. The Plant Cell: tpc.00121.02019.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: John Browse and James G. Wallis (2019) Arabidopsis Flowers Unlocked the Mechanism of Jasmonate Signaling. Plants 2019, 8, 285; doi:10.3390/plants8080285
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Rawale, Kanwardeep S., Muhammad A. Khan, and Kulvinder S. Gill. 2019. The Novel Function of the Ph1 Gene to Differentiate Homologs from Homoeologs Evolved in Triticum Turgidum Ssp. Dicoccoides via a Dramatic Meiosis-Specific Increase in the Expression of the 5B Copy of the C-Ph1 Gene. Chromosoma, September, 110. https://doi.org/10.1007/s00412-019-00724-6.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Aharen, I., Habash, S.H., Gleason, C., Inada, M., Grundler, F.M.W. Elashry, A., Heterodera schachtii glutathione peroxidase (HsGPx) is a parasitism protein, Journal of Plant Disease and Protection, 1-8, DOI 10.1007/s41348-019-00256-2.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Vieira, P. and Cynthia Gleason, Plant-parasitic nematode effectors  insights into their diversity and new tools for their identification. (2019) Current Opinion in Plant Biology, 50:37-43.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Aaron J Ogden, Jacqueline M McAleer, and Michael L Kahn (2019) Characterization of the Sinorhizobium meliloti HslUV and ClpXP Protease Systems in Free-Living and Symbiotic States. Journal of Bacteriology 2019 201:e00498-18. doi: 10.1128/JB.00498-18.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: D.A. Gacek, C.-P. Holleboom, S. Tietz, H. Kirchhoff, P.J. Walla (2019) The role of the PsbS protein for carotenoid-chlorophyll coupling in grana thylakoids. FEBS Letters doi: 10.1002/1873-3468.13586.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: N. Pang, X. Gu, S. Chen, H. Kirchhoff, H. Lei, S. Roje (2019) Exploiting mixotrophy for improving productivities of biomass and co-products of microalgae. Renewable and Sustainable Energy Reviews 112, 450-460.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: H. Kirchhoff (2019) Chloroplast ultrastructure in plants. Invited review article for New Phytologist 223, 565-574.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: N Pang, Y. Xie, HM Oung, BV Sonawane, X Fu, H Kirchhoff, A Cousins, S Chen (2019) Regulation and stimulation of photosynthesis of mixotrophically cultured Haematococcus pluvialis by ribose. Algal Research 39, 101443.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: H. Koochak. S. Puthiyaveetil, D. Mullendore, M. Li, H. Kirchhoff (2019) The structural and functional domains of plant thylakoid membranes. The Plant Journal 97, 412-429. Reseach highlight and cover.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Samec D., Pierz V., Srividya N., Wuest M., Lange B.M. (2019) Assessing chemical diversity in Psilotum nudum (L.) Beauv., a pantropical whisk fern that has lost many of its fern-like characters. Front. Plant Sci. 10, 868. doi: 10.3389/fpls.2019.00868
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ahkami A.H., Wang W., Wietsma T.W., Winkler T., Lange I., Jansson C., Lange B.M., McDowell N.G. (2019) Metabolic shifts associated with drought-induced senescence in Brachypodium. Plant Sci. 289, 110278. doi 10.1016/j.plantsci.2019.110278
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: 1. Roy A, Zhai Y, Ortiz J, Neff MM, Mandal B, Mukherjee SK and Pappu HR (2019) Multiplexed editing of a begomovirus genome restricts escape mutant formation and disease development PLoS ONE 14: (10).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Tian, L., H.-L. Chou, L. Zhang, S.-H. Hwang, S.R. Starkenburg, K. Doroshenk, T. Kumamaru, and T.W. Okita (2018) RNA-binding protein RBP-P is required for glutelin and prolamine mRNA localization in rice endosperm cells. Plant Cell, 30: 2529-2552. DOI: https://doi.org/10.1105/tpc.18.00321.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: 1. Hwang, S.-K. Salvinder Singh, Jitendra Maharana, Samhita Kalita, Aytug Tuncel, Tanmayee Rath, Debashish Panda, Mahendra Kumar Modi, and Thomas W. Okita. (2019) Mechanism underlying heat stability of the rice endosperm cytosolic ADP-glucose pyrophosphorylase. Frontiers in Plant Biology, 11 February 2019 | https://doi.org/10.3389/fpls.2019.00070.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Cakir, Bilal, Li Tian, Naoko Crofts, Hong-Li Chou, Kaan Koper, Chun-Yeung Ng,, Aytug Tuncel, Mahmoud Gargouri, Seon-Kap Hwang, Naoko Fujita and Thomas W. Okita. (2019) Re-programming of gene expression in the ADPglucose over-accumulating CS8 rice line reveals a new player in starch biosynthesis. Plant J. 97:1073-1088 https://doi.org/10.1111/tpj.14180.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Tian, L., H.-L. Chou, L. Zhang, and T.W. Okita. (2019) Targeted endoplasmic reticulum localization of storage protein mRNAs requires the RNA-binding protein RBP-L. Plant Physiol. 179:1111-1131. DOI: https://doi.org/10.1104/pp.18.01434.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Chou, H.-L., L. Tian, M. Fukuda, T. Kumamaru, and T.W. Okita. (2019) The rice storage protein mRNAs as a model system for RNA localization in higher plants. Plant Sci., 284:203-211. https://doi.org/10.1016/j.plantsci.2019.04.014.
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Peng H and Neff MM (2019) CCA1 and ATAF2 differentially suppress cytochrome P450-mediated brassinosteroid inactivation in Arabidopsis Journal of Experimental Botany (in press).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Zhai Y, Peng H, Neff MM and Pappu HR (2019) Putative auxin and light responsive promoter elements from the Tomato spotted wilt tospovirus genome, when expressed as cDNA, are functional in Arabidopsis Frontiers in Plant Science 10; 804.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: J.H. Artz, O.A. Zadvornyy, D.W. Mulder, S.M. Keable, A.E. Cohen, M.W. Ratzloff, S.G. Williams , B. Ginovska, N. Kumar, J. Song, S.E. McPhillips, C.M. Davidson, A.Y. Lyubimov, N. Pence, G.J. Schut, A.K. Jones, S.M. Soltis, M.W.W. Adams, S. Raugei, P.W. King, J.W. Peters Tuning catalytic bias of hydrogen gas producing hydrogenases J. Am. Chem. Soc. revised manuscript submitted (2019).
  • Type: Journal Articles Status: Under Review Year Published: 2019 Citation: 1. M.-H. Ryu, J. Zhang, T. Toth, D. Khokhani, B.A. Geddes, F. Mus, A. Garcia-Costas, J.W. Peters, P.S. Poole, J.-M. An�, C.A. Voigt Control of nitrogen fixation in bacteria that associate with cereals Nat. Microbiol. revised manuscript submitted (2019).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: J.L. Yuly, C.E. Lubner, P. Zhang, D.N. Beratan, J.W. Peters Electron bifurcation: progress and grand challenges Chem. Commun. 55:11823-11832 (2019).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: F. Mus F, D.R. Colman, J.W. Peters, E.S. Boyd Geobiological feedbacks, oxygen, and the evolution of nitrogenase Free Radic. Biol. Med. 140:250-259 (2019).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: B.R. Streit, J.R. Mattice, G.A. Prussia, J.W. Peters, J.L. DuBois The reactive form of a C-S bond-cleaving, CO2-fixing flavoenzyme J. Biol. Chem. 294:5137-5145 (2019).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: G.J. Schut, N. Mohammed-Raseek, M. Tokmina-Lukaszewska, D.W. Mulder, D.M. Nguyen, G.L. Lipscomb, J.P. Hoben, A. Patterson, C.E. Lubner, P.W. King, J.W. Peters, B. Bothner, A-F. Miller, M.W.W. Adams A general catalytic mechanism for electron bifurcating electron transfer flavoproteins (ETFs) J. Biol. Chem. 294:3271-3283 (2019).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: J.A. Laureantia, G.W. Buchkoa, S. Katipamulaa, Q. Sua, O.A. Zadvornyy, J.W. Peters, M. OHagana Protein scaffold activates catalytic CO2 hydrogenation by a rhodium bis(diphosphine) complex ACS Catal. 9:620-625 (2019).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: J.W. Peters, D.N. Beratan, B. Bothner, R.B Dyer, C.S. Harwood, Z.M. Heiden, R. Hille, A.K. Jones, P.W. King, Y. Lu, C.E. Lubner, S.D. Minteer, D.W. Mulder, S. Raugei, G.J. Schut, L.C. Seefeldt, M. Tokmina-Lukaszewska, O. Zadvornyy, P. Zhang, M.W. W. Adams A new era for electron bifurcation Curr. Opin. Chem. Biol. 47:32-28 (2018).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: L.C. Seefeldt, J.W. Peters, D.N. Beratan, B. Bothner, S.D. Minteer, S. Raugei, and B.M. Hoffman Control of electron transfer in nitrogenase Curr. Opin. Chem. Biol. 47:54-59 (2018).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 1. Yuan, P. Yang, T. and Poovaiah, B.W. (2018). Calcium Signaling-Mediated Plant Response to Cold Stress. Int. J. Mol. Sci. 2018, 19(12), 3896; https://doi.org/10.3390/ijms19123896.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Yoo, C.Y, Mano, N, Finkler, A., Weng, H., Day, I.S., Reddy, A.S.N., Poovaiah, B.W., Fromm, H., Hasegawa, P.M. and Mickelbart, M.V. (2019). Allosteric control of the GTL1 transcription factor by Ca2+/calmodulin transduces water-deficit stress to the repression of stomatal development on drought stress acclimation. Scientific Reports, a journal of the Nature Research group, 9, 115.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Pi, E., Li, H., Xu, J, Zhu, C., Jiang, J. He, L., Lu, H., Wang, H., Poovaiah, B.W. and Du, L. (2019). Enhanced salt tolerance of soybean inoculated with rhizobia is correlated with the decreased phosphorylatin of GmMYB183 and altered flavonoid biosynthesis. Molecular and Cellular Proteomics, pp.mcp-RA119. DOI: 10.1074/mcp.RA119.001704.
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Ghimire B, Hulbert S, Steber C, Garland-Campbell K, Sanguinet KA. (2019) Characterization of root traits for improvement of spring wheat in the Pacific Northwest, Agronomy Journal, accepted
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Cong W, Miao Y, Xu L, Zhang Y, Yuan C, Wang J, Zhuang T, Lin X, Jiang L, Wang N, Ma J, Sanguinet KA, Liu B, Rustgi S, Ou X. (2019) Transgenerational Memory of Gene Expression Changes Induced by Heavy Metal Stress in Rice (Oryza sativa L.) BMC Plant Biology 19 (1); 282
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ma X, Sanguinet KA, Jacoby P. (2019) Performance of direct root-zone deficit irrigation on Vitis vinifera Cabernet Sauvignon production and root distribution in southcentral Washington, Agricultural Water Management, 221, 47-57
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Brew-Appiah RAT, Peracchi LM, Sanguinet KA. (2019) Never the two shall mix: Robust indel markers to ensure the fidelity of two pivotal and closely-related accessions of Brachypodium distachyon, Plants, (8) 6: 153
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: 1. Zhang Z, Fu T, Lui Z, Wang X, Xun H, Li G, Ding B, Dong Y, Lin X, Sanguinet KA, Liu B, Wu Y. (2019) Extensive changes in gene expression and alternative splicing due to homeologous exchange in rice segmental allopolyploids, Theoretical and Applied Genetics, 132 (8); 22952308
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Clark I, Jones SS, Reganold JP, Sanguinet KA, Murphy KM. (2019) Agronomic performance of perennial grain genotypes in the Palouse region of the Pacific Northwest, USA, Frontiers in Sustainable Food Systems, 3, 39
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lehman T, Sanguinet KA. (2019) Auxin and cell wall crosstalk as revealed by the Arabidopsis thaliana cellulose synthase mutant radially swollen 1, Plant and Cell Physiology, 60 (7): 1487-1503
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Brew-Appiah RAT, Sanguinet KA. (2018) Considerations of AOX functionality revealed by critical motifs and unique domains, International Journal of Molecular Sciences, 19(10), 2972
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Handakumbura PP, Brow K, Whitney IP, Zhao K, Sanguinet KA, Lee SJ, Olins J, Romero-Gamboa SP, Harrington MJ, Bascom CJ, MacKinnon KJ-M, Veling MT, Liu L, Lee JE, Vogel, JP, OMalley RC, Bezanilla M, Bartley LE, Hazen SP. (2018) Brachypodium distachyon SWAM1 is a positive regulator of secondary cell wall synthesis and biofuel feedstock attributes and is not found in the Brassicaceae, The Plant Journal, 96 (3), 532-545
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Joseph H. Lynch, Na Sa, Sompop Saeheng, Nadia Raffaelli, and Sanja Roje (2018) Characterization of a non-nudix pyrophosphatase points to interplay between flavin and NAD(H) homeostasis in Saccharomyces cerevisiae. PLoS One 13(6):e0198787. doi: 10.1371/journal.pone.0198787.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Hinojosa L., Sanad M., Jarvis D., Steel P., Murphy K., Smertenko A. (2019) Impact of heat and drought stress on peroxisome proliferation in quinoa. Plant J. 99: 1144-1158. doi: 10.1111/tpj.14411
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Schmidt S.A., Smertenko A.* (2019) Identification and characterization of a land-plant specific microtubule nucleation factor, MACET4. J. Cell Science. 132, jcs232819. doi:10.1242/jcs.232819.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Sanad M., A. Smertenko A., and Garland-Campbell K.A. (2019) Differential Dynamic Changes of Reduced Trait Model for Analyzing the Plastic Response to Drought Phases: A Case Study in Spring Wheat. Frontiers Plant Science. 10:504. doi.org/10.3389/fpls.2019.00504.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Smertenko A. (2019) Phosphoinositides Break Microtubule Dynamics Symmetry in the Phragmoplast. Trends Cell Biology, 29, 449-451, https://doi.org/10.1016/j.tcb.2019.03.002.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Jewell, JB, Tanaka K (2019) Transcriptomic perspective on extracellular ATP signaling: a few curious trifles. Plant Signal. Behav. 14 (11): e1659079 doi:10.1080/15592324.2019.1659079.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Marcec MJ, Gilroy S, Poovaiah BW, Tanaka K (2019) Mutual interplay of Ca2+ and ROS signaling in plant immune response. Plant Sci. 283: 343-354 doi:10.1016/j.plantsci.2019.03.004.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Jewell JB, Sowders JM, He R, Willis MA, Gang DR, Tanaka K (2019) Extracellular ATP shapes a defense-related transcriptome both independently and along with other defense signaling pathways. Plant Physiol.179 (3): 1144-1158 doi:10.1104/pp.18.01301.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hadwiger LA, Tanaka K (2018) DNA damage and chromatin conformation changes confer nonhost resistance: a hypothesis based on effects of anti-cancer agents on plant defense responses. Front. Plant Sci. 9: 1056 doi:10.3389/fpls.2018.01056.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ha, P.T., He, R., Killiny, N., Brown, J.K., Omsland, A., Gang, D.R., and Beyenal, H. (2019). Host-free biofilm culture of Candidatus Liberibacter asiaticus, the bacterium associated with Huanglongbing. Biofilm 1, 100005.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Molki, B., Ha, P.T., Mohamed, A., Killiny, N., Gang, D.R., Omsland, A., and Beyenal, H. (2019). Physiochemical changes mediated by Candidatus Liberibacter asiaticus in Asian citrus psyllids. Scientific reports 9, 1-9.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Molki, B., Ha, P.T., Cohen, A.L., Crowder, D.W., Gang, D.R., Omsland, A., Brown, J.K., and Beyenal, H. (2019). The infection of insect vector by bacterial plant pathogen" Candidatus Liberibacter solanacearum" is associated with altered vector physiology. Enzyme and Microbial Technology, 109358.
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Zasada, I., Dandurand, LM, Gleason, C., Hagerty, C., Ingham, R. Plant-parasitic nematodes of Idaho, Oregon, and Washington (Pacific Northwest), Book chapter in Parasitic Nematodes in Sustainable Agriculture of North America, editors Sergei Subbotin and John Chitamber, June 2018.
  • Type: Book Chapters Status: Published Year Published: 2019 Citation: Ramachandran SR, Kumar S, Tanaka K (2019) Quantification of extracellular ATP in plant suspension cell cultures. In Plant Innate Immunity: Methods and Protocols (W. Gassmann, ed.). Methods Mol. Biol., Vol 1991, pp. 43-54 (Chapter 5). Humana, New York, NY doi:10.1007/978-1-4939-9458-8_5.
  • Type: Theses/Dissertations Status: Published Year Published: 2018 Citation: Singh, K (2018) Evolution of Ph1 gene and its use in wheat improvement. Ph.D. thesis. Crop Science. Washington State University.
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Khan, MA (2019) Promoter characterization and protein identification of the Ph1 gene that regulates chromosome pairing in wheat (Triticum aestivum). Ph.D. thesis. Crop Science. Washington State University.
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Lehman, TA (2019) Deciphering factors that drive root morphogenesis: cell wall-phytohormone crosstalk and a kinase involved in root hair development. Ph.D. thesis. Washington State University.
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Weed, RA (2019) Metabolomic changes in grasses due to biotic and abiotic factors. Ph.D. thesis. Molecular Plant Sciences. Washington State University.
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Westenskow, S (2019) Using CRISPR-Cas9 to Knockout Brassinosteroid Inactivating Genes in Arabidopsis thaliana. M.S. thesis Crop Sciences. Washington State University
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: Sharma-Koirala, P (2019) The AHL Gene Family and its Role in Flowering Time, Hypocotyl Length, Seed Size and Seed Weight in Arabidopsis. M.S. thesis Crop Sciences. Washington State University
  • Type: Theses/Dissertations Status: Published Year Published: 2019 Citation: York, ZB (2019) Regulators of seedling root-growth rate and characterization of SALP1 in Brachypodium distachyon. M.S. thesis. Washington State University
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Dr. Kahn: Steering Committee for the North American Symbiotic Nitrogen Fixation Meeting to be held in June, 2020, Madison, WI
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Dr. Kahn: Prestigious Graduate Fellowships Workshop, Washington State University, 2018, 2019
  • Type: Other Status: Published Year Published: 2018 Citation: Dr. Kahn: Director, WSU-NIH Protein biotechnology Training Program
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited session chair for the opening session on Chemistry and Regulation of Nitrogenase International Nitrogen Fixation Conference, Wuhan China, October 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Vice Chair of International Steering Committee for the International Nitrogen Fixation Conference, Wuhan China, October 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Co-organizer and host, Telluride Science Research Center Workshop, Biological and Bioinspired Redox Catalysts, Telluride, CO, July 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Co-organized and hosted a global virtual symposium and workshop at the Noble Research Institute, Toward a Roadmap for Sustainable N-Use in Agriculture, Ardmore, OK, March 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Discussion Leader, Gordon Research Conference on Metals in Biology, Ventura, CA, January 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Session Chair, Western Photosynthesis Conference, Fair Harbor, WA, January 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Roje: A session chair for the 2019 Plant Synthetic Biology Conference (San Jose, CA)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Bates: Kansas State University, Department of Biochemistry and Molecular Biophysics Seminar Series (Manhattan, KS)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Bates: Noble Research Institute Seminar Series (Ardmore, OK)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Bates: University of North Texas, Biodiscovery Institute Seminar Series (Denton, TX)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Bates: 9th European Symposium on Plant Lipids (Marseille, France)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Bates: 36th Annual Mid-Atlantic Plant Molecular Biology Society Meeting (Beltsville, MD).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Browse: VIB Conference on Regulatory Oxylipins. University of Ghent, Belgium. Sex and the Single Hormone. April 1  4, 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gang: Culturing Candidatus Liberibacter asiaticus, the Causal Agent of Citrus Greening Disease (Huanglongbing, HLB). International Research Conference on Huanglongbing (IRCHLB) (13 March 2019, Riverside, CA).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gang: Culturing the Unculturable: Progress in the Fight Against Citrus Greening Disease (Huanglongbing) invited seminar, Department of Plant Pathology, Washington State University (4 February 2019).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gang: Hemp on the Palouse? Rotary Club of Whitman County. (8 May 2019)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gang: Location, Location, Location: Application of MS-Imaging in the Life Sciences. PacMass Spring Meeting. (26 March 2019)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gang: Location, Location, Location: Application of MS-Imaging in the Life Sciences. Bruker Scientific User Group, Seattle Area. (26 March 2019)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gill: Punjab Agricultural University, Luhdiana, India (Presenter: Kanwardeep Singh) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Dr. Gill: Heat Stress Symposium, CSSA, Baltimore, MD, Nov 4-7, 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Dr. Gill: Graduate student training: The USA perspective. IAUA Golden Jubilee International Conference" held during 23-25 November, 2018 at NASC, Pusa, New Delhi.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Dr. Gill: University of Maryland, MD, April 28, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Kirchhoff: University of Leiden (Netherlands) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gleason: University of Wisconsin, Madison, the XVII Congress on Molecular Plant-Microbe Interactions, Glasgow, Scotland
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Gleason: Florida State University, Gainesville.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Kirchhoff: CEPLAS summer school (Maria an der Aue, Germany) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Kirchhoff: Biophysical Society-Bioenergetics Subgroup (Baltimore, MD, USA) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Lange: Annual Conference of the Phytochemical Society of North America (Johnson City, TN) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Lange: Terpnet Conference (Halle, Germany) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Lange: Botanikertagung, (Rostock, Germany) 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: Washington State Crop Improvement, two talks (Moscow, ID)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: 20th Annual Noxious Weed Conference, two talks (Moses Lake WA)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: CSANR Outreach Meeting (Pullman WA)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: WSU School of Molecular Biosciences (Pullman WA)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: The Othello Sandhill Crane Festival (Othello WA)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Neff: WSU Land Legacy Dinner (Spangle WA)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Opening Keynote Session, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH, 7/21/19-7/26/19
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Telluride Science Research Center workshop, Biological and Bioinspired Redox Catalysts Telluride, CO, 7/16/19-7/20/19
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Dr. Peters: DOE Solar Photochemistry P.I. Meeting Gaithersburg, MD, 6/3/19-6/5/19
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Cluster of Excellence in Plant Sciences Transatlantic Summer School  Frontiers in Plant Sciences, Maria in der Aue, Germany, 5/27/19-5/31/19
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: 12th International Conference on Hydrogenases, Lisbon, Portugal, 3/31/19-4/4/19
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Seminar, Department of Chemistry & Chemical Biology, Cornell University, Nov. 14, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Seminar, School of Molecular Sciences, Arizona State University, Sept. 20, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Seminar, Max Planck Institute for Chemical Energy Conversion, M�lheim an der Ruhr, May 29, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Seminar, Fakult�t f�r Biol. und Biotech., Photobiotechnologiea, Ruhr Universit�t Bochum, May 28, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Peters: Invited Seminar, Freiburg Institute for Advanced Studies, Albert-Ludwigs-Univeristat Freiburg, May 24, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Sanguinet (delivered by Dr. Lehman), International Brachypodium Meeting, Huesca, Spain, June 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Smertenko: American Society of Plant Biologists Annual Meeting in San-Jose in July, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Smertenko: Mid-West Plant Cell Dynamics Meeting, June 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Tanaka: Food Safety and Bio-Environmental Science, Kagoshima University, Kagoshima, Japan. August 26, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dr. Tanaka: Department of Biochemistry and Molecular Biology, Saitama University, Saitama, Japan. August 2, 2019


Progress 02/20/18 to 09/30/18

Outputs
Target Audience:The main audience is the scientific community working on the different aspect of plant biology covered by this MPS Hatch umbrella. Knowledge gained by this consortium will help solve pressing agricultural problems which are relevant for Washington growers as well as farmers worldwide. Several members of the consortium actively collaborate with the Washington crop Commissions. These interactions will be used to communicate findings of the proposal to stakeholders. Changes/Problems:In 2018 a new PI joint the MPS Hatch umbrella consortium: Dr. Phil Bates, Institute of Biological Chemistry. What opportunities for training and professional development has the project provided?The project provided training to PostDocs, PhD students, M.S. students, undergraduate students and high school students that totals to 76 students and PostDocs as summarized in the following table. The training included practicing and improving different scientific techniques and presenting results and data on research conferences, meetings, workshops, and symposia. PostDocPhDMSundergradhigh school Browse12 Gang2 Gill333 Gleason111 Kahn23 Kirchhoff22 Neff26242 Okita233 Peters111 Poovaiah11 Sanguinet11131 Roje1 Smertenko212 Tanka32131 Total14274274 How have the results been disseminated to communities of interest?The main venue for dissemination of products of the MPS Hatch umbrella project is publication as journal articles and presentations at national and international conferences and meetings. The long list of 42 published papers in peer-reviewed scientific journals complemented by two book chapters, and the high number of invited oral presentations together with the organization of international and national meetings emphasizes the excellent scholarly achievement of this project. This is complemented by completing five Ph.D. dissertations during the reporting period. Furthermore, presentations were given to stakeholders and framers. What do you plan to do during the next reporting period to accomplish the goals?All project participants will continue and develop their projects as described in the narrative of the proposal. Some specific activities are listed below: Browse: Continue to investigate the potential for PDCT regulation in producing high-oleic oils. Gang: Further characterization of enzymes in the salvinorin A pathway will be cloned and characterized by the new graduate student. Gill:The alien introgression via virus induced gene silencing of the C-Ph1 gene will be continued to develop a novel method for the alien gene transfers. To further study evolution of the Ph1 gene, various progenitor species will be evaluated to accurately determine the presence of various features responsible for the chromosome pairing control mechanism. Antibodies for the C-Ph1 gene proteins will be made and will be used to show the number and size of the proteins being made by the gene and to immuno-localize the proteins. Gleason: Continue to identify key root-knot nematode effectors and their plant targets in Arabidopsis and potato. Hadwiger: I plan to continue assessing the molecular basis of how the Pep13 modification improves the induction of plant immunity Kirchhoff: Further unraveling architectural dynamics in thylakoid membranes with special focus on the sub-localization of the cytochrome b6f complex and on the role of thylakoid membrane ion channels and transporter. Neff:Continue to study the role of BR-inactivating genes, and their transcriptional regulation, with regard to seedling and adult plant development. Also continue to describe the role of dominant-negative alleles inAHLgenes with regard to regulating seed size and seedling development. Continue to clone and characterize VRN-related genes in Kentucky Bluegrass. Finally, continue to work with WSU to build a new Native Grass Ecology and Breeding Farm to facilitate breeding new varieties of grasses native to North America. Okita:Complete studies on the role of OsTudor-SN in protein body formation and seed development. Complete studies on the role of RBP-L in storage protein RNA localization. Complete studies on the interaction of RBP-Q with RBP-P.Initiate studies to show that the membrane trafficking factor, NSF, is closely associated with RBP-P biochemically and cytologically in developing rice endosperm. Conduct gas exchange and physiological studies of wild-type, pho1 mutant line, and transgenic pho1 lines expressing wild-type and Pho1ΔL80. Obtain direct biochemical evidence that Pho1 is present on PS1 complexes isolated from thylakoid membranes. Quantify PS1 complexes by quantitative immunoblotting using anti-PsaC and PsaB antibodies. Peters:Determining the mechanism of the Fix electron bifurcating enzyme that supplies low potential electrons for nitrogen fixation in some model diazoptrophs. Provide further insights into the mechanism of respiratory protection in model aerobic diazoptrophs. Provide additional insights into the determinants of ammonia dependent regulatory response and the specific determinants for ammonia excreting phenotypes and examining the efficacy and stability of ammonia excreting strains during crop growth. Pooviah: Continue studies on the mechanistic aspects of transcription factor (AtSR1) and its role in plant immune/defense response. Specifically, (1) continue to evaluate fluctuations in intracellular calcium in response to microbial signal and study how the signal is amplified and produces the final response; (2) continue studies on NPR1:AtSR1 interactions and study the role in immune response; (3) explore the primary regulon of AtSR1; and (4) include studies on calciumsignaling-mediated plant response to abiotic stresses such as cold since it is becoming clear that calcium signaling plays a crucial role in conferring cold tolerance in plants. Sanguinet:Prove BUZZ is a bonafide kinase using in vitro kinases assays and by initiating phosphoproteomics. Validate Luciferase lines and begin HTP phenomics suppressor screen. Roje: Finish up characterization of the bacterial enzyme. Start confirming some of the gene candidates from Medicago. Smertenko: Measure expression of genes that control peroxisome proliferation in our mutants. Analyze performance of mutants under the drought stress. Tanaka:Further investigate the downstream signaling following extracellular ATP recognition byits receptor P2K1. Working on phospho-proteomics and interactomics for the DAMP receptor P2K1.Continuously try for identification of novel elicitors from infected potato tissues.

Impacts
What was accomplished under these goals? The project is divided into 6 research areas: A1-A3 (Products and Synthesis) and B1-B3 (Defense and Signaling). Accomplishments in these areas are summarized below. A1 Primary metabolism (Kirchhoff, Okita) A deeper understanding of thylakoid membranes dynamics and off the role of starch phosphorylase (Pho1) was achieved together with identifying new functions ofstorage protein mRNAs transportproteins. It was discovered that the central photosynthetic electron transport complex (cyt b6f) undergoes dynamic redistributions between stacked and unstacked thylakoid membrane domains inArabidopsis. This lateral reorganization fine tunes linear and cyclic electron transport processes in plants to adjust ATP production by photosynthesis to the actual metabolic demand. Furthermore, functional studies on knock-out mutants of thylakoid ion transporter/channels demonstrated that they have specific functions in photo-protective high energy quenching (qE).Tudor-SN, RBP-P, and RBPL were shown to be multi-functional in not only being required for the targeting of storage protein mRNAs to specific ER subdomains but also for a variety of developmental and growth processes. The interaction of the rice starch phosphorylase, Pho1, to the photosystem I terminal electron donor, PsaC, was validated and shown to modulate photosynthesis. A2 Secondary metabolism (Browse, Roje, Lewis, Gang, Lange) By employing directed evolution, GWAS, and diverse transformation strategies, the quality and quantity of specialized plant secondary products were improved and metabolic pathways that lead to their production were unraveled. In detail, more active variants of the enzyme desaturase (DES9*) were selected inArabidopsisthat were able to reduce the amount of unhealthy saturated fatty acids. However, plant lines with the lowest levels of saturates, exhibited defective embryo development, lower oil seed content, and reduced germination rates. Repeating these experiments withCamelinarevealed that reductions in saturated fatty acids did not produce negative phenotypes as observed inArabidopsis. For the flavin biosynthesis, a recombinant bacterial enzyme was biochemically characterized inSinorhizobium meliloti. Furthermore, candidate genes responsible for variations in flavin contents inM. truncatulahave been identified. In continuing to optimize the level of phenylethanol glucoside production in transgenic poplar, we have been able to increase its amounts considerably in leaf and stem tissues to circa 5.5% and 6.5%, respectively. This is a very high level of accumulation of this specialty chemical. Efforts to produce basil flavone in yeast were successful that led to filing a provision application patent. A3 Roots / Products and Synthesis (Sanguinet, Kahn) The root hairless BUZZ (cell division kinases) mutants, which have increased root growth rates were completely characterized forArabidopsisandBrachypodiumand the subcellular localization determined. Thesubcellular localization and complementation revealed that BUZZ is localized and functional in the nucleus.In the subproject on symbiotic interaction between legumes and N2-fixing bacteria, new genes fromSinorhizobiumwere discovered that increases nodulation. The bacterial proteasome complexes and attached proteins were investigated. It was found that defects in proteasomes affect bacterial resistance to various stresses and decrease symbiotic productivity. Efforts to increase the amount of an unusual metabolite (PCO) that accumulates in a Fix+ ineffective symbiosis were successful. B1 Signals and Hormones (Poovaiah, Tanaka, Neff) In this section, biochemical, molecular biological, transcriptomic, phenotyping, bioinformatic, and CRIPR/CAS-9 techniques were applied to study the function of calcium, danger signals, and hormones for plant responses to environmental cues and seed productivity.We investigated calcium transients and signaling events that orchestrate plant-microbe interactions, specifically how calcium acts as a second messenger connecting the perception of microbe signals to the establishment of immune responses in plants. We proposed a model to summarize our work on AtSR1/CAMTA3-mediated immune response and calcium's role in it.We also documented that during plant-pathogen interactions, plants have to reallocate their resources to defend against invading organisms.In brief, AtSR1/CAMTA3 plays a critical role in balancing plant growth and immunity.A new signaling crosstalk was identified between extracellular ATP (a danger signal also known as a DAMP) and other plant hormones (Jasmonate, Salicylate, and ethylene). The DAMP elicitors enhance plant resistance against a broad range of plant pathogens. These results allow for the improvement of current crop protection from various pathogens and pests. Furthermore, methods have been established to measure early defense responses in plants against several pathogens that enables to explore conserved plant immune responses. A transcription factor as repressor of the brassinosteroid (BR)-inactivating geneshas been identified involved inregulating seedling development. In addition,a BR-inactivating quadruple mutant was phenotyped. It has beendemonstrated that thesob3-6allele ofSOB3is the only dominant-negative mutation that leads to increased seed size.It was discovered that theSOFL gene family inArabidopsisis larger than previously expected. Furthermore, vernalization genes in Kentucky bluegrass have been identified. Finally, planning has been started with WSU to design a new Native Grass Ecology and Breeding Farm. B2 Stress Response (Hadwiger, Smertenko, Gill) Significant progress was made to unravel the role of the Pep13 signal peptide that is released from pathogens inside potato tissue from a transaminase enzyme. This immunity response induction has been shown to be enhanced by modifying the 13 amino acid sequence. The modification of Pep13 enhancing signal strength will be used to enhance the disease resistance responses in crops. A large-scale screen for peroxisomal mutants in the collection of wheat Kronos TILLING lines has been established. Out of 1300 screened lines 50 were identified with high or low peroxisome abundance. It was demonstrated that this approach for identification of peroxisome abundance mutants is successful.It was shownthat the Ph1 gene involved in chromosome pairing control evolved in the emmer wheat after the polyploidization event.It has been discovered that a dramatic increased abundance of one of three splice variants of the C-Ph1 gene that shows differential accumulation during meiotic stages is responsible for chromosome pairing control. Finally, successfulrecombination between wheat and rye chromosomes was observed by transiently silencing the C-Ph1 gene via virus induced gene silencing. B3 Roots / Defense and Signaling (Peters, Gleason) A first detailed mechanism for an electron bifurcation mechanism in nitrogen fixing bacteria was discovered that is key to understanding the physiology and biochemistry of electron transfer reactions in these organisms. This includes redefining the rate limiting step for nitrogen catalysis. This allows a better understanding of the bioenergetics of multiple cofactor systems leading to deeper insights into the mechanism of redox/energy homeostasis and the regulation of nitrogen fixation in oxygen respiring model diazoptrophs. Bacterial strains were generated that excrete ammonia and promote plant growth under nitrogen limiting conditions. In the root-knot nematode project, transcriptomic analysis led to the identification of 167 genes that were induced in parasitic nematodes encoding secreted proteins. A yeast two hybrid screen of potato cDNA library yielded potential effector targets in potato. This led to the discovery of nematode effector MiPFN3 that interacts with plant actin and affects actin filament polymerization. The nematodes secrete MiPFN3 to facilitate parasitism.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Daniel Lunn, James G. Wallis and John Browse (2018) Overexpression of Seipin1 Increases Oil in Hydroxy-Fatty Acid Accumulating Seeds. Plant Cell Physiology 59:205-214.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Kanwardeep Singh, Johar Singh, Suruchi Jindal, Gaganjot Sidhu, Amandeep Dhaliwal and Kulvinder Gill (2018). PIN1 structural and functional evolution across monocots and dicots. Functional and Integrative Genomics 19:29 https://doi.org/10.1007/s10142-018-0625-9
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Nathan P. Grant. Amita Mohan, Devinder Sandhu, and Kulvinder S. Gill 2018. Inheritance and genetic mapping of the reduced height (Rht18) gene in wheat. Molecular Plant Breeding 7, 58; doi:10.3390/plants7030058
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Ragupati Nagarajan and Kulvinder S. Gill 2018. Evolution of Rubisco activase gene in plants. Plant Molecular Biology 96: 69 https://doi.org/10.1007/s11103-017-0680-y.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Bhavya P. Mishra, Rajeev Kumar, Amita Mohan, and Kulvinder S. Gill 2018. Conservation and divergence of Starch Synthase III genes of monocots and dicots. PLOS ONE 12(12): e0189303 https://doi.org/10.1371/journal.pone.0189303
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Leelarasamee N, Zhang L, Gleason C. 2018. The root-knot nematode effector MiPFN3 disrupts plant actin filaments and promotes parasitism.PLoS Pathog. 2018 Mar 15;14(3):e1006947. doi: 10.1371/journal.ppat.1006947
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hadwiger, L. A., Tanaka, T (2018). DNA damage and chromatin conformation changes confer nonhost resistance: A hypothesis based on effects of anti-cancer agents on plant defense responses. Front. Plant Sci. 9:1056-1072
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Koochak. S. Puthiyaveetil, D. Mullendore, M. Li, H. Kirchhoff (2018) The structural and functional domains of plant thylakoid membranes. The Plant Journal 97, 412429 https://doi.org/10.1111/tpj.14127
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: D. Charuvi, A. Gal, E. Aviv-Sharon, E. Shimoni, H. Kirchhoff, J, Farrant, R. Nevo, V. Kiss, Z. Reich (2018) Chloroplast breakdown during dehydration of a homoiochlorophyllous resurrection plant proceeds via senescence-like processes. Environ. Exp. Bot. 157, 100-111.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: R. H�hner, J.V. Marques, T. Ito, Y. Amakura, A.D. Budgeon, Jr., L.B. Davin, H. Kirchhoff, N.G. Lewis, 2018. Modulating arogenate dehydratase gene expression in Arabidopsis thaliana: Photosynthetic energy conversion and metabolism ramifications. Plant Physiology 177, 115-131.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: H. Kirchhoff (2018) Structure-function relationship in photosynthetic membranes: Challenges and Emerging Fields. Plant Science 266, 76-82.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Tayengwa R, Zhao J, Peirce CF, Werner BE and Neff MM (2018) Synopsis of the plant-specific SOB-FIVE-LIKE (SOFL) gene family G3: Genes, Genomes, Genetics 8:1281-129. doi: 10.1534/g3.118.200040.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Chou, H.-L., L. Tian, T. Kumamaru, and T.W. Okita (2018) Multifunctional RNA binding protein OsTudor-SN in storageprotein mRNA transport and localization. Plant Physiol. 175:1608-1623
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yang, Y., Hong-Li Chou, Andrew J. Crofts, Laining Zhang, Li Tian, Haruhiko Washida, Masako Fukuda, Toshihiro Kumamaru, Oliver J. Oviedo, Shawn Starkenburg, and Thomas W. Okita. (2018) Selective sets of mRNAs localize to extracellular paramural bodies in the rice glup6 mutant line. J. Exp. Bot. 69(21):50455058 https://doi.org/10.1093/jxb/ery297.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Tian, L., H.-L. Chou, L. Zhang, S.-H. Hwang, S.R. Starkenburg, K. Doroshenk, T. Kumamaru, and T.W. Okita (2018) RNAbinding protein RBP-P is required for glutelin and prolamine mRNA localization in rice endosperm cells. Plant Cell, 30:25292552 DOI: https://doi.org/10.1105/tpc.18.00321.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: J.W. Peters, D.N. Beratan, B. Bothner, R.B Dyer, C.S. Harwood, Z.M. Heiden, R. Hille, A.K. Jones, P.W. King, Y. Lu, C.E. Lubner, S.D. Minteer, D.W. Mulder, S. Raugei, G.J. Schut, L.C. Seefeldt, M.Tokmina?Lukaszewska, O. Zadvornyy, P. Zhang, M.W. W. Adams A new era for electron bifurcation Curr. Opin. Chem. Biol. 47:32-38 (2018).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: L.C. Seefeldt, J.W. Peters, D.N. Beratan, B. Bothner, S.D. Minteer, S. Raugei, and B.M. Hoffman. 2018. Control of electron transfer in nitrogenase Curr. Opin. Chem. Biol. 47:54-59
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: L.C. Seefeldt, B.M. Hoffman, J.W. Peters, S. Raugei, D.N. Beratan, E. Antony, D.R. Dean. 2018. Energy transduction in nitrogenase Acc. Chem. Res. 51:2179-2186.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: J.W. Peters, D.N. Beratan, G.J. Schut, M.W.W. Adams. 2018. On the nature of organic and inorganic centers that bifurcate electrons, coupling exergonic and endergonic oxidation?reduction reactions Chem. Commun. 54:4091-4099
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: S.M. Keable, O.A. Zadvornyy, L. Johnson, B. Ginovska, A.J. Rasmussen, K. Danyal, B.J. Eilers, G.A.Prussia, A.X. LeVan, S. Raugei, L.C. Seefeldt. J.W. Peters. 2018.Characterization of the P1+ intermediate state of nitrogenase P cluster J. Biol. Chem. 293:9629-9635
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: F. Mus, A.B. Alleman, N. Pence, J.W. Peters. 2018. Exploring the alternatives of biological nitrogen fixation Metallomics 10:523-528
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: H. Yu, C.?H. Wu, G.J. Schut, G. Zhao, J.W. Peters, M.W. W. Adams, Huilin Li. 2018. Structure of an Ancient Respiratory System Cell 173:1636-1649
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: S. Poudel, D.R. Colman, K.R. Fixen, R.N. Ledbetter, Y. Zheng, N. Pence, J.W. Peters, L.C. Seefeldt, C.S. Harwood, E.S. Boyd. 2018. Electron transfer to nitrogenase in different genomic and metabolic backgrounds J. Bacteriol. 200(10): e00757-17 pii: JB.00757?17. doi: 10.1128/JB.00757?17.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Berry, L., Patterson, A., Pence, N., Peters, J. W. and Bothner, B. (2018). Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins. Bio-protocol 8(6): e2769. DOI: 10.21769/BioProtoc.2769.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: H.D. Duan, C.E. Lubner, M. Tokmina?Lukaszewska, G.H. Gauss, B. Bothner, P.W. King, J.W. Peters, A.F. Miller. 2018. Distinct flavin properties underlie flavin?based electron bifurcation within a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris J. Biol. Chem. 293:4688-4701.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: S.M. Keable, J. Vertemara, O.A. Zadvornyy, B.J. Eilers, K. Danyal, A.J. Rasmussen, L. De Gioia, G. Zampella, L.C. Seefeldt, J.W. Peters. 2018. Structural characterization of the nitrogenase MoFe protein with the substrate acetylene trapped near the active site J. Inorg. Biochem. 180:129-134.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Poovaiah, B.W. and Du, Liqun. 2018. Calcium signaling: decoding mechanism of calcium signatures. New Phytologist. 217:1598-1609.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Pi, E., Zhu, C., Fan, W., Huang, Y, Qu, L., Li, Y., Zhao, Q., Qiu, L., Wang, H., Poovaiah, B.W. and Du, L. 2018. Quantitative Phosphoproteomic and Metabonomic Analyses Reveal GmMYB173 Optimizes Flavonoid Metabolism in Soybean under Salt Stress. Mol Cell Proteomics mcp.RA117.000417. doi:10.1074/mcp.RA117.000417.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yuan, P., Tanaka, K., Du, L. and Poovaiah B.W. 2018. Calcium signaling in plant autoimmunity: A guard model for AtSR1/CAMTA3-mediated immune response. Molecular Plant 11, 637-639.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yuan, P., Du, L. and Poovaiah, B.W. 2018. Ca2+/Calmodulin-Dependent AtSR1/CAMTA3 Plays Critical Roles in Balancing Plant Growth and Immunity. Int. J. Mol. Sci. 2018, 19(6), 1764; https://doi.org/10.3390/ijms19061764.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Lynch JH, Sa N, Saeheng S, Raffaelli N, Roje S. Characterization of a non-nudix pyrophosphatase points to interplay between flavin and NAD(H) homeostasis in Saccharomyces cerevisiae. PLoS One. 2018 Jun 14;13(6):e0198787. doi: 10.1371/journal.pone.0198787. eCollection 2018.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Smertenko A. (2018) Phragmoplast Expansion: the Four-Stroke Engine that Powers Plant Cytokinesis. Current Opinion Plant Biology, 46:130-137 doi: 10.1016/j.pbi.2018.07.011.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Brew-Appiah RAT, Sanguinet KA. (2018) Considerations of AOX Functionality Revealed by Critical Motifs and Unique Domains, International Journal of Molecular Sciences, 19(10), 2972.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Brew-Appiah RAT, York ZB, Krishnan V, Roalson EH, Sanguinet KA. (2018) Genome-wide identification and analysis of the ALTERNATIVE OXIDASE gene family in diploid and hexaploid wheat, PLoS One, e0201439.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: S.E. Partovi, F. Mus, A.E. Gutknecht, H.A. Martinez, B.P. Tripet, B.M. Lange, J.L. DuBois, J.W. Peters. 2018. Coenzyme M biosynthesis in bacteria involves phosphate elimination by a unique member of the aspartase/fumarase superfamily J. Biol. Chem. 293:5236-5246.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Handakumbura PP, Brow K, Whitney IP, Zhao K, Sanguinet KA, Lee SJ, Olins J, Romero-Gamboa SP, Harrington MJ, Bascom CJ, MacKinnon KJ-M, Veling MT, Liu L, Lee JE, Vogel, JP, OMalley RC, Bezanilla M, Bartley LE, Hazen SP. (2018) Brachypodium distachyon SWAM1 is a positive regulator of secondary cell wall synthesis and biofuel feedstock attributes and is not found in the Brassicaceae, The Plant Journal, 96 (3), 532-545.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Wu Y, Sun Y, Sun S, Li G, Wang J, Wang B, Lin X, Huang M, Gong Zhiyun, Sanguinet KA, Zhang Z, Liu B. (2018) Aneuploidization under segmental allotetraploidy in rice and its phenotypic manifestation, Theoretical and Applied Genetics, 1-13.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Martinez S, Thompson A, Wen N, Murphey L, Sanguinet KA, Steber C, Garland-Campbell K (2018) Registration of the Louise/Alpowa Wheat Recombinant Inbred Line Mapping Population, Journal of Plant Registrations 12(2):282-287 doi:10.3198/jpr2017.08.0053crmp.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Liu D, Zefroosh N, Hines K, Fang W, Sanguinet KA, Goldner L, Baskin TI (2018) Cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon, Scientific Reports, 7, 15111.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: DeShields JB, Bomberger RA, Woodhall JW, Wheeler DL, Moroz N, Johnson DA, Tanaka K (2018) On-site molecular detection of soil-borne phytopathogens using a portable real-time PCR system. J. Vis. Exp. e56891 doi:10.3791/56891.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Tripathi D, Tanaka K (2018) A crosstalk between extracellular ATP and JA signaling pathways. Plant Signal. Behav. 13:e1432229 doi:10.1080/15592324.2018.1432229.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Tripathi D, Zhang T, Koo AJ, Stacey G, Tanaka K (2018) Extracellular ATP acts on jasmonate signaling to reinforce plant defense. Plant Physiol. 176:511-523 doi:10.1104/pp.17.0147.
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Hadwiger, L. A. (2018). How plants resist disease- a focus on "nonhost resistance of pea to a bean pathogen. http://openaccessebook.com/nonhost-resistance.html
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: H. Kirchhoff, R. Yarbrough, Evaluation of lipids and membranes for the study of photosynthetic superorganization. In: Methods in Molecular Biology. (Editor, S. Covshoff) 1770, Springer Press, 305-316.
  • Type: Theses/Dissertations Status: Other Year Published: 2018 Citation: Ogden, AP (2018) Metabolic progression during development of symbiotic nitrogen fixation in the Medicago-Sinorhizobium symbiosis and the role of HslUV and ClpXP protease machinery. Ph.D. thesis. Molecular Plant Sciences. Washington State University.
  • Type: Theses/Dissertations Status: Other Year Published: 2018 Citation: Adolphsen, KN. (2018) Rhizobial genes that contribute to effective symbiosis with legumes. Ph.D. thesis. Molecular Biosciences. Washington State University.
  • Type: Theses/Dissertations Status: Other Year Published: 2018 Citation: Chen X. 2018. ()-Kolavenyl diphosphate synthase and acetyltransferase involved in salvinorin a biosynthesis pathway in Salvia. PhD Dissertation. Washington State University, Pullman, WA.
  • Type: Theses/Dissertations Status: Other Year Published: 2018 Citation: Kixson, KK (2018) Network and multi-omics analyses of Arabidopsis arogenate dehydratase knock-out and over-expression mutants. Washington State University, Pullman, WA.
  • Type: Theses/Dissertations Status: Other Year Published: 2018 Citation: Yuan, P. 2018. Calcium Signaling in Plants: Role of AtSR1 in Immune Response. PhD Dissertation. Washington State University, Pullman, WA.