Source: LOUISIANA STATE UNIVERSITY submitted to NRP
GERM CELL AND EMBRYO DEVELOPMENT AND MANIPULATION FOR THE IMPROVEMENT OF LIVESTOCK
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1015296
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
W-3171
Project Start Date
May 10, 2018
Project End Date
Jun 4, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
LOUISIANA STATE UNIVERSITY
202 HIMES HALL
BATON ROUGE,LA 70803-0100
Performing Department
School of Animal Science
Non Technical Summary
A greater understanding of the molecular and cellular mechanisms regulating early embryonic development is critical to address problems of embryonic lethality observed in agricultural production. Combining technologies such as in vitro fertilization, next-generation sequencing, bioinformatics analysis, mathematical modeling and state-of-art genomicediting provides phenomenal opportunities to understand the role of key molecular regulators controlling viability ofgametes and embryos. In vitro embryo production could be timed to be used to increase flexibility in animal production.Genomic selection through NGS based screens in combination with OPU, the useof sexed semen, and further epigenetic selection of early embryos will lead to a faster rate of genetic improvement, thereforeincreasingefficiency of livestock production.
Animal Health Component
(N/A)
Research Effort Categories
Basic
70%
Applied
(N/A)
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3013310105070%
3033410104030%
Knowledge Area
303 - Genetic Improvement of Animals; 301 - Reproductive Performance of Animals;

Subject Of Investigation
3310 - Beef cattle, live animal; 3410 - Dairy cattle, live animal;

Field Of Science
1040 - Molecular biology; 1050 - Developmental biology;
Goals / Objectives
Understand the biology and underlying mechanisms of gamete development, fertilization, and embryogenesis. Refine methods for production of genetically enhanced animals to improve livestock production efficiency.
Project Methods
Use low-input whole genome bisulfite sequencing andChIP-seq throughput technologies to profile methylome and chromatin architectureof bovine oocytes and embryos.Develop a network-based analyses approach to discover master regulator genes in early embryo development.Develop genome-phenome association approach to identify key maternal and paternal drivers.Determine molecular and cellular mechanisms by which maternal and paternal drivers regulate preimplantation development using state-of-art genomic editing technologies.

Progress 05/10/18 to 06/04/18

Outputs
Target Audience:Farm animal producers and reproductive physiologists in industry and academia. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A PhD studentis trained under the decribed research activities.Someresearch data described will be part of hisdissertation. How have the results been disseminated to communities of interest?Continue with the state protocols. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? It was confirmed that stimulation with FSH prior to OPU collection results in greater developmental competence compared to non-stimulated OUP or slaughterhouse derived oocytes. RNA seq data with oocytes from these methods of collection identified differentially expressed genes in both the germinal vesicle and metaphase II stages which can be investigated as molecular markers of developmental competence. The effect of heat stress on the developmental competence and global gene expression profile of oocytes through the transition from spring to summer under Louisiana conditions were assessed. RNA seq analysis identified the genes that were differentially expressed as a result of heat stress at the GV stage and MII stage. This information provided new insight into the molecular mechanisms of detrimental conditions (heat stress) on bovine oocytes which may help to reveal master regulators controlling oocyte competence. DNA methylation is crucial in the epigenetic regulation of mammalian embryogenesis. Using both single cell/embryo reduced representation bisulfite sequencing (RRBS) and single-cell/embryo whole genome bisulfite sequencing (WGBS), stage-specific genome-wide dynamics of DNA methylation was characterized in bovine sperm, immature, in vivo and in vitro matured oocyte as well as in vivo developed early embryo stages.This study provides the first comprehensive analysis of the global dynamics of DNA methylation in bovine gametes and single early embryos. These data will serve as an important reference base for embryos produced by assisted reproduction such as in vitro fertilization and cloning as well as models for human early embryo development.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: 1. Duan J, Zhu L, Dong H, Zheng X, Jiang Z, Chen J, Tian XC. Abundance of mRNA for histone variants, histone- and DNA-modifying enzymes in bovine in vivo and in vitro oocytes and embryos. Sci Rep. 2019: 9 (1), 1217.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: 2. Duan JE, Flock K, Jue N, Zhang M, Jones A, Seesi S, Mandoiu I, Pillai S, Hoffman M, ONeill R, Zinn S, Govoni K, Reed S, Jiang H, Jiang Z, Tian XC. Dosage compensation and gene expression of the X chromosome in sheep. G3:GenesGenomesGenetics. 2019. Jan 9;9(1):305-314. PMID: 30482800.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 3. Duan J, Shi W, Jue N, Jiang Z, Kuo L, ONeill R, Wolf E, Dong H, Zheng X, Chen J; Tian XC. Dosage Compensation of the X Chromosomes in Bovine Germline, Early Embryos and Somatic Tissues. Genome Biology and Evolution, 2018. Dec 19. PMID: 30566637.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 4. Duan JE, Zhang M, Flock K, Seesi SA, Mandoiu I, Jones A, Johnson E, Pillai S, Hoffman M, McFadden K, Jiang H, Reed S, Govoni K, Zinn S, Jiang Z, Tian XC. Effects of Maternal Nutrition on the Expression of Genomic Imprinted Genes in Ovine Fetuses. Epigenetics. 2018 Jul 27. doi: 10.1080/15592294.2018.1503489. PMID: 30051747.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 5. Jiang Z, Lin J, Dong H, Zheng X, Marjani SL, Duan J, Ouyang Z, Chen J, Tian XC. DNA methylomes of bovine gametes and in vivo produced preimplantation embryos. Biology of Reproduction. 2018 Jun 14. PMID: 29912291.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 6. Wang T, Babayev E, Jiang Z, Li G, Zhang M, Esencan E, Horvath T, Seli E. Mitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre-implantation embryos. Aging Cell. 2018 May 30:e12784. PMID: 29851234.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 7. Wang L*, Jiang Z*, Huang D, Duan J, Huang C, Sullivan S, Vali K, Yin Y, Zhang M, Wegrzyn J, Tian XC, Tang Y. JAK/STAT3 regulated global gene expression dynamics during late-stage reprogramming process. BMC Genomics. 2018 Mar 6;19(1):183. PMID: 29510661.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: 1. Jiang Z, Diaz F.A, Gutierrez E.J, Foster B.A, Hardin P.T, Bondioli K.R. Effect of heat stress on oocyte developmental competence and global gene expression dynamics in Bos Taurus Crossbred beef cows. 44th Annual conference of IETS, New Orleans, LA, 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: 2. Duan J*, Jiang Z*, Alqahtani F, Mandoiu I, Dong H, Zheng X, Marjani S, Chen J, Tian XC. Whole genome bisulfite sequencing of bovine gametes and in vivo early embryos. 44th Annual conference of IETS, New Orleans, LA, 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 3. Jiang Z, Lin J, Dong H, Zheng X, Marjani S, Duan J, Ouyang Z, Chen J, Tian X. DNA methylomes of bovine gametes and in vivo produced preimplantation embryos. 51th Annual conference of Society for the Study of Reproduction (SSR), New Orleans, LA, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 4. Duan JE*, Jiang Z*, Alqahtani F, Mandoiu I, Dong H, Zheng X, Marjani S, Chen J and Tian XC. 2018. Whole genome bisulfite sequencing of bovine in vivo gametes and preimplantation embryos. Poster presentation at Epigenetics & Chromatin Meeting, Cold Spring Harbor Laboratory, Sept 11-15, 2018. Cold Spring Harbor, New York.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 5. Duan J, Shi W Jue N, Jiang Z, Kuo L, O'Neill R, Wolf E, Dong H, Zheng X, Chen J, Tian X. Dosage Compensation of the X Chromosomes in Bovine Germline, Early Embryos and Somatic Tissues. 44th Annual conference of IETS, Bangkok, Thailand, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 6. Duan JE, Zhang M, Flock K, Seesi SA, Mandoiu I, Jones AK, Pillai SM, Hoffman ML, Jiang H, Reed SA, Govoni KE, Zinn SA, Jiang Z and Tian XC. Effects of Maternal Nutrition on Genomic Imprinting in Fetal Sheep. Oral and poster presentation at Plant & Animal Genome XXVI conference; Jan 13-17, 2018; San Diego, CA.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 7. Foster BA, Gutierrez EJ, Jiang Z, Bondioli KR. The effect of bovine oocyte retrieval methods on oocyte gene expression and competence. 51th Annual conference of SSR, New Orleans, LA, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 8. Jiang Z, Duan J, Wang T, Zhang M, Tian XC, Seli E. Evaluating mitochondrial stress response gene CLPP regulated DNA methylome dynamics in femal reproductive aging. 75th Annual conference of ASRM, Colorado, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 9. Wang T, Babayev E, Jiang Z, Zhang M, Esencan E, Seli E. Rapamycin partially rescues oocyte dysfunction in mice deficient for mitochondrial stress response protein CLPP. 75th Annual conference of ASRM, Colorado, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 10. Esencan E, Jiang Z, Wang T, Zhang M, Bener MB, R. Scott III, Seli E. Impaired mitochondrial stress response in cumulus cells is associated with increased apoptosis and accelerated follicular depletion. 75th Annual conference of ASRM, Colorado, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 11. Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, R. Scott III, Seli E. MFN1 is required for follicle development, oocyte maturation, and female fertility. 75th Annual conference of ASRM, Colorado, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 12. Jiang Z, Logsdon DM, Schoolcraft WB, Krisher RL, Yuan Y. Single cell gene expression profiling of trophoblast generated from self-organizing human embryos in vitro.75th Annual conference of ASRM, Colorado, 2018.