Progress 03/15/18 to 03/14/22
Outputs
Target Audience:Undergraduate and graduate students in my lab as they are engaged in research and lab meetings. STEM majors from Delaware State University as my students and post-doctoral scientist presented the project results at the annual research day and summer research symposiums from 2019 to 2022. Interested participants at annual National Association of Plant Breeders (NAPB) meetings, 2020 American Society for Horticultural Sciences meeting. Readers of the published research articles from this project. Additionally, Blueberry growers and enthusiasts from Mid-Atlantic region who participated in our blueberry workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?A post-doctoral research associate Dr. Krishnanad P. Kulkarni was hired for accomplishing the project goals. The research project provided him the learning experience to handle large number of samples and data handling. This project also provided peer mentoring opportunities for post doc, graduate students, technician and undergraduate students in the lab and also facilitated them to participate in extension activities by way of help organize workshops and interact with clientele. This project also provided opportunities to the involved students and staff in crafting scientific communications as they were presented research findings at the annual University symposiums, professional meetings, gave elevator speeches, developed thesis and manuscripts. How have the results been disseminated to communities of interest?The graduate Student Byron Manzanero presented research findings at the annual DSU research day, defended thesis to the public in Spring 2021. Some students from Delaware State University and The University of Rutgers were the audience. We were able to generate four peer reviewed research articles from this grant along with other grant funds. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
High chill blueberry (Vaccinium corymbosum L.) is a temperate fruit crop noted for desirable fruit quality. However, highbush cultivars are being subjected to higher summer temperatures, as well as the increased consumer demand, has blueberry production expanding to almost all continents with varying climates. With the warming climate in temperate zones northern highbush blueberry will likely require higher heat tolerance. V. darrowii, a diploid evergreen subtropical blueberry species native to the southern gulf coast may offer heat tolerance. The diploid parents NJ88-14-03, NJOPB-15, NJOPB-8 and NJ88- 12-41, F1; BNJ05-218-9 and BNJ05-237-8 and around 350 F2 plants from BNJ16-4 population were grown, genomic DNA was extracted and made ready for illumina sequencing. Plants were subjected to heat stress conditions and scoring was performed. These reciprocal crosses comprising 320 plants were subjected to high-temperature stress at 40°C for four days in the growth chamber and evaluated for chlorophyll fluorescence parameters (quantum yield [QY] and rapid fluorescence transient assay [OJIP]) and vegetation indices (Normalized Difference Vegetation Index [NDVI], Simple Ratio Index [SR] and Greenness Index [GI]). In blueberry, chlorophyll fluorescence parameters were used as an indicator of thermal tolerance and helped identify thermal-tolerant and -sensitive cultivars. GBS libraries were prepared by digesting DNA with a single restriction enzyme ApeK1 to genotype the 260 plants from the BNJ16-4 population and 60 plants from the BNJ16-5 population. We generated a large number of high-quality SNPs and successfully mapped 2.8 million GBS-obtained sequence reads per sample (Kulkarni et al. 2021) to the genome sequence of the tetraploid variety Draper. A total of 1,323 significant SNPs (p<0.01) associated with the chlorophyll fluorescence parameters and associated traits were identified by GWAS. The number of SNPs ranged from a minimum of 76 for Greenness Index to a maximum of 196 for Quantum Yield. To identify the candidate genes and their functions, 245 SNPs that were significantly associated with more than one high-temperature stress-tolerance traits were selected for further analysis.
Publications
- Type:
Journal Articles
Status:
Under Review
Year Published:
2022
Citation:
Byron R. Manzanero, Krishnanand P. Kulkarni, Nicholi Vorsa, Umesh K Reddy, Purushothaman Natarajan, Sathya Elavarthi1, Massimo Iorizzo, and Kalpalatha Melmaiee. Genomic relationships among wild and cultivated blueberry species. Journal of Advanced Research.
- Type:
Journal Articles
Status:
Other
Year Published:
2022
Citation:
Krishnanand P. Kulkarni, Nicholi Vorsa, Umesh K Reddy, Purushothaman Natarajan, Sathya Elavarthi1, Massimo Iorizzo, and Kalpalatha Melmaiee. Identification of candidate genes for high temperature stress tolerance in blueberries.
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Progress 03/15/20 to 03/14/21
Outputs
Target Audience:Undergraduate and graduate students in my lab as they are engaged in research and lab meetings. STEM majors from Delaware State University as my students presented the project results at the research day and summer research symposium. Interested participants at National Association of Plant Breeders (NAPB) meeting. Additionally, Blueberry growers and enthusiasts from Mid-Atlantic region who participated in our blueberry workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We have hired a post-doctoral research associate Dr. Krishnanad Kulkarni for this project. The research project provided him the learning experience to handle large number of samples and data handling. This project also provides peer mentoring opportunities for post doc, graduate students, technician and undergraduate students in the lab and also facilitated them to participate in extension activities by way of help organize workshops and interact with clientele. How have the results been disseminated to communities of interest?The results are presented at various meetings by the participation members. Graduate student Ms. Jodi Callwood has presented her research at the 1890 Association of Research Directors (ARD) meeting in April 2019 and also will be presented as posters at National Association of Plant Breeders's meeting at Pine Mountain, GA in August 2019 by PI Dr. Melmaiee and graduate student. The graduate Student Byron Manzanero presented his thesis research at the annual DSU research day. Many graduate students and undergrads from the college are the audience. What do you plan to do during the next reporting period to accomplish the goals?Rework on some of the bioinformatics analysis to increase the confidentiality of the candidate genes and prepare manuscripts for publication.
Impacts
What was accomplished under these goals?
High chill blueberry (Vaccinium corymbosum L.) is a temperate fruit crop noted for desirable fruit quality. However, highbush cultivars are being subjected to higher summer temperatures, as well as the increased consumer demand, has blueberry production expanding to almost all continents with varying climates. With the warming climate in temperate zones northern highbush blueberry will likely require higher heat tolerance. V. darrowii, a diploid evergreen subtropical blueberry species native to the southern gulf coast may offer heat tolerance. The diploid parents NJ88-14-03, NJOPB-15, NJOPB-8 and NJ88- 12-41, F1; BNJ05-218-9 and BNJ05-237-8 and around 350 F2 plants from BNJ16-4 population were grown, genomic DNA was extracted and made ready for illumina sequencing. Plants were subjected to heat stress conditions and scoring was performed. These reciprocal crosses comprising 320 plants were subjected to high-temperature stress at 40°C for four days in the growth chamber and evaluated for chlorophyll fluorescence parameters (quantum yield [QY] and rapid fluorescence transient assay [OJIP]) and vegetation indices (Normalized Difference Vegetation Index [NDVI], Simple Ratio Index [SR] and Greenness Index [GI]). In blueberry, chlorophyll fluorescence parameters were used as an indicator of thermal tolerance and helped identify thermal-tolerant and -sensitive cultivars. GBS libraries were prepared by digesting DNA with a single restriction enzyme ApeK1 to genotype the 260 plants from the BNJ16-4 population and 60 plants from the BNJ16-5 population. We generated a large number of high-quality SNPs and successfully mapped 2.8 million GBS-obtained sequence reads per sample (Kulkarni et al. 2021) to the genome sequence of the tetraploid variety Draper. A total of 1,323 significant SNPs (p<0.01) associated with the chlorophyll fluorescence parameters and associated traits were identified by GWAS. The number of SNPs ranged from a minimum of 76 for Greenness Index to a maximum of 196 for Quantum Yield. To identify the candidate genes and their functions, 245 SNPs that were significantly associated with more than one high-temperature stress-tolerance traits were selected for further analysis.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Callwood, Jodi, Kalpalatha Melmaiee, Krishnanand P. Kulkarni, Amaranatha R. Vennapusa, Diarra Aicha, Michael Moore, Nicholi Vorsa, Purushothaman Natarajan, Umesh K. Reddy, and Sathya Elavarthi. "Differential Morpho-Physiological and Transcriptomic Responses to Heat Stress in Two Blueberry Species." International Journal of Molecular Sciences 22, no. 5 (2021): 2481.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Kulkarni, Krishnanand P., Nicholi Vorsa, Purushothaman Natarajan, Sathya Elavarthi, Massimo Iorizzo, Umesh K. Reddy, and Kalpalatha Melmaiee. "Admixture Analysis Using Genotyping-by-Sequencing Reveals Genetic Relatedness and Parental Lineage Distribution in Highbush Blueberry Genotypes and Cross Derivatives." International Journal of Molecular Sciences 22, no. 1 (2021): 163.
- Type:
Conference Papers and Presentations
Status:
Submitted
Year Published:
2020
Citation:
Kalpalatha Melmaiee*, Krishnanand P. Kulkarni, Jodi Callwood, Nicholi Vorsa and Sathya Elavarthi. Blueberries: What we know about their heat stress tolerance. American association of Horticultural Society (ASHS) annual conference August 10-14, 2020, Virtual.
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Progress 03/15/19 to 03/14/20
Outputs
Target Audience:Scientific community attendies of National association of Plant Breeders 2019 annual meeting at Pine Mountain, GA and 2020 Plant and Animal Genomics ( PAG), San Diego, CA. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We have hired a post-doctoral research associate Dr. Krishnanad Kulkarni for this project. The research project provided him the learning experience to handle large number of samples and data handling. This project also provides peer mentoring opportunities for post doc, graduate students, technician and undergraduate students in the lab and also facilitated them to participate in extension activities by way of help organize workshop and interact with clientele. How have the results been disseminated to communities of interest?Results were presented at the Natitional Association of Plant Breeders 2019 annual meeting at Pine Mountain, GA in August by PI and Post Doctoral Scientist presented at the Plant and Animal Genomics ( PAG) 2020 conference at San Diego, CA. What do you plan to do during the next reporting period to accomplish the goals?Genomic data analysis and scoring for heat stress tolerance traits will be continued. Manuscripts will be prepared from this project.
Impacts
What was accomplished under these goals?
High chill blueberry (Vaccinium corymbosum L.) is a temperate fruit crop noted for desirable fruit quality. However, highbush cultivars are being subjected to higher summer temperatures, as well as the increased consumer demand, has blueberry production expanding to almost all continents with varying climates. With the warming climate in temperate zones northern highbush blueberry will likely require higher heat tolerance. V. darrowii, a diploid evergreen subtropical blueberry species native to the southern gulf coast may offer heat tolerance. The diploid parents NJ88-14-03, NJOPB-15,NJOPB-8 and NJ88- 12-41, F1; BNJ05-218-9 and BNJ05-237-8 and around 350 F2 plants from BNJ16-4 population were grown, genomic DNA was extracted and made ready for illumina sequencing. Plants were subjected to heat stress conditions and scoring was performed. We have also collected fruits from the grand parents, F1 fruiting F2, and placed them in -80 for analysis of fruit volatiles, flavonols, organic acids and have taken data on F2 for fall senescence. Genotyping- by- Sequencing (GBS) protocol was optimized and libraries were prepared for sequencing and sequenced. Sequence quality control followed by alignment to existing blueberry, grape and Arabidopsis were performed. Identification of SNPs, LD blocks and QTL maps with one-year data were performed. Blueberry workshop was organized to interested farmers from Delaware and neighboring counties. The focus was on factors to be considered before establishing blueberry plantations including soil and water pH level testing.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
https://napb2019.uga.edu/files/2019/08/napb-program-with-abstracts.pdf
pag.confex.com
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Progress 03/15/18 to 03/14/19
Outputs
Target Audience:Our taget audience are blueberry growers from Delaware and surrounding regions, undergraduate and graduate students from the College of Agriculture, Science andTechnology at Delaware State University and Rutgers University. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We have hired a post-doctoral research associate Dr. Krishnanad Kulkarni for this project. The research project provided him the learning experience to handle large number of samples and data handling. This project also provides peer mentoring opportunities for post doc, graduate students, technician and undergraduate students in the lab and also facilitated them to participate in extension activities by way of help organize workshop and interact with clientel. How have the results been disseminated to communities of interest?The results are presented at various meetings by the participation members. Graduate student Ms. Jodi Callwood has presented her research at the 1890 Association of Research Directors ( ARD) meeting in April 2019 and also will be presented as posters at National Association of Plant Breeders's meeting at Pine Mountain, GA in August 2019 by PI Dr. Melmaiee and graduate student. What do you plan to do during the next reporting period to accomplish the goals?In the coming year we are planning on performing Genotyping-by-Sequencing (GBS), analyze the results, identify polymorhisms. Repeat the heat stress experiments for identification of reliable QTLs.
Impacts
What was accomplished under these goals?
High chill blueberry (Vaccinium corymbosum L.) is a temperate fruit crop noted for desirable fruit quality. However, highbush cultivars are being subjected to higher summer temperatures, as well as the increased consumer demand, has blueberry production expanding to almost all continents with varying climates. With the warming climate in temperate zones northern highbush blueberry will likely require higher heat tolerance. V. darrowii, a diploid evergreen subtropical blueberry species native to the southern gulf coast may offer heat tolerance. The diploid parents NJ88-14-03, NJOPB-15,NJOPB-8 and NJ88-12-41, F1; BNJ05-218-9 and BNJ05-237-8 and around 350 F2 plants from BNJ16-4 population were grown, genomic DNA was extracted and made ready for illumina sequencing. Plants were subjected to heat stress conditions and scoring was performed. We have also collected fruit from the grand parents, F1 fruiting F2, and placed them in -80 for analysis of fruit volatiles, flavonols, organic acids and have taken data on F2 for fall senescence. Blueberry workshop was organized to interested farmers from Delaware and neighboring counties. The focus was on factors to be considered before establishing blueberry plantations including soil and water pH level testing.
Publications
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