Source: CORNELL UNIVERSITY submitted to NRP
BIOLOGICAL CONTROL OF ARTHROPOD PESTS AND WEEDS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1015223
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
NE-1332
Project Start Date
Jan 25, 2018
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Entomology
Non Technical Summary
Due to severe problems with pest resistance to insecticides, greenhouse crop producers are actively interested in using biocontrol for key pests such as Western flower thrips (WFT) and fungus gnats (FG). Certain insect-killing nematode species are produced commercially and sold to growers to apply to the soil where the nematodes attack and kill the soil-dwelling stages of pests such as thrips and fungus gnats. Application method is simple and very similar to typical pesticide application methods. Though EPNs are available for WFT and FG, use can be unreliable for unclear reasons and adoption is scattered. Our primary goal is to identify the most effective entomopathogenic (insect-killing) nematodes (EPNs) to use against WFT and FG, and conditions that enhance or hinder efficacy. EPN efficacy is influenced by EPN species and strain, application dose, frequency, and timing, site-specific environmental conditions, and common crop production practices. We will determine the most efficacious EPN for each pest, including persistence in growing mix; effect of growing mix moisture, temperature, and various common growing mixes on the most effective EPN; optimal application rates, frequency, and timing. Results from initial lab studies will be confirmed by small-scale greenhouse studies, followed by trials under commercial greenhouse conditions.
Animal Health Component
90%
Research Effort Categories
Basic
10%
Applied
90%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21521231130100%
Goals / Objectives
Augmentation programs involving repeated rearing and release
Project Methods
Nematode strains of each species will be evaluated under variable conditions. Commercial strains typically have lost the ability to persist more than a few days whereas native strains available at Cornell University have retained persistence capabilities. We will evaluate the efficacy of both commercial and native strains of Steinernema carpocapsae, S. feltiae, Heterorhabditis bacteriophora, and H. indica against WFT prepupae and pupae and FG larvae. These tests will be conducted in increasing complex arenas starting with lab assays on filter paper in petri dishes and finishing with potted plants in the greenhouse. Variables tested will include 1) an array of soil mixes, 2) a range of soil moistures and 3) a range of temperatures. Effective species/strains will then be tested in a series of small-scale greenhouse studies with caged plants to evaluate their persistence, as well as test optimal application rates, frequencies, and timing. Finally, with continued funding in a subsequent year, trials under commercial greenhouse conditions will be used to test the most promising results. Trials will be run for both cool-weather spring crops conditions as well as warmer end-of-summer/fall poinsettia production. Experimental treatments will be compared with grower-chosen conventional practices at a minimum of six greenhouse operations. Costs of each treatment at each location will be recorded and compared.

Progress 01/25/18 to 09/30/18

Outputs
Target Audience:This project was intended to aid greenhouse growers in their pest management efforts using insect-killing nematodes. Cooperative extension professionals can also use this information to provide to their grower clientele. Entomology and nematoology researchers may also use this information. Companies that produce nematodes for sale to growers may use this information. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Sanderson, J.P., E. Shields, L. White, & A. Testa. 2017. Effect of irrigation method on persistence of fungus gnat (sciarid) control by the nematode Steinernema feltiae. Proceedings of the IOBC-WPRS Working Group "Integrated Control in Protected Crops, Temperate Climate" international meeting, 04-08 June 2017, Niagara Falls, Ontario, Canada. Editors: Bruno Gobin and Rose Buitenhuis. ISBN 978-92-9067-309-5 [IX + 254 pp., p. 112] https://www.iobc-wprs.org/members/shop_en.cfm?mod_Shop_detail_produkte=170 Sanderson, J.P. 2018. "Biological Warfare against Greenhouse Pests: Some New Developments." Lower Mainland Horticultural Improvement Association (LMHIA) Growers' Short Course. Jan. 25. Abbotsford, British Columbia, Canada. 85 attendees. 1 hr. Sanderson, JP, AR Giesmann. 2018. "Nematodes: Nice, not Naughty." Capital District Bedding Plant Conference. Albany, NY. Jan. 11. 90 attendees. 45 min. Sanderson, JP, AR Giesmann. 2018. "Nematodes: Nice, not Naughty." Long Island Greenhouse & Floriculture Conference. Jan. 16. 105 attendees. 1 hr. Sanderson, JP, AR Giesmann. 2018. "Nematodes: Nice, not Naughty." Greenhouse Session, Empire State Producers Expo. Jan. 17. Syracuse, NY. 30 attendees. 45 min. Sanderson, JP, AR Giesmann. 2018. "Nematodes: Nice, not Naughty." Western NY Greenhouse Conference. Eden, NY. 65 attendees. 1 hr. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Growers are growing greenhouse crops at cooler temperatures to reduce heating costs. To evaluate the efficacy of Steinernema feltiae nematodes at cool temperatures, we grew potted pansy plants in replicated cages within replicated walk-in growth chambers set at either constant 12.8C (cool treatment) or constant 23.9C (warm treatment) and 12:12 L:D. Cages were then infested equally with large numbers of adult fungus gnats for 2 weeks, or were uninfested to serve as controls. The nematodes were then drenched at recommended rate into the pots twice at a 2-week interval. Adult fungus gnats captured on yellow sticky traps were then counted in each cage until no adults were found for 2 consecutive days. Results show that, relative to fungus gnat numbers of the control cages, fungus gnat numbers were reduced by 40% in the warm treatment and by 60% in the cool treatment, indicating that the nematodes were still effective at the cool temperatures. Additional greenhouse tests were done to evaluate how often nematodes should be applied, and whether the irrigation method (overhead vs. sub-irrigation) affect nematode persistence in the pots. Results were inconclusive due to the eventual unwanted presence of extraneous fungus gnats somehow infesting the cages that were intended to exclude them. However, fungus gnats were consistently suppressed for at least two weeks after a single application, suggesting that growers need not make weekly nematode applications. Biweekly applications should suffice under low to moderate fungus gnat levels. The result from the application frequency was evaluated in a commercial greenhouse. Nematodes were applied either weekly or biweekly for 6 weeks and adult fungus gnats and Western flower thrips were counted on sticky traps. Result showed no differences in trap catches during the trial, suggesting that control was not improved with weekly applications over biweekly applications. The efficacy of two commercial nematode species, Steinernema feltiae and S. carpocapsae, were compared against larval fungus gnats at three application rates, 1000, 5000, and 10,000 nematodes per 10cm container. These rates span below and above typical commercial application rates. At 23.9C, fungus gnats were significantly suppressed at all rates of S. feltiae well below that of any S. carpocapsae treatment. Control was 100% with 10,000 nematodes per container for S. feltiae vs. 65% for the same rate of S. carpocapsae. However, preliminary results from another project are suggesting that S. feltiae cannot survive temperatures exceeding 35C, whereas S. carpocapsae can. Thus at high temperatures, growers may want to switch to S. carpocapsae.

Publications