Progress 03/01/18 to 02/29/24
Outputs
Target Audience:Our target audience of scientists and nutrition professionals was reached with the publication/presentation below: Sholola MJ, Miller JL, Bilbrey EA, Torok M, Novotny J, Francis DM, Mace TA, Cooperstone JL. The Effects of a Lycopene-Rich Tomato Juice Enhanced With Soy Isoflavones on Inflammation in Individuals With Obesity. Curr Dev Nutrition 2023:7(1);101489. https://doi.org/10.1016/j.cdnut.2023.101489 Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This grant has provided training opportunities for a graduate student and technician. How have the results been disseminated to communities of interest?Results have been disseminated via presentations and conference proceedings, and will be published in peer reviewed journals. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The overarching goal of this project is to understand how particular dietary components from foods can alter markers of inflammation in humans, while monitoring metabolic changes to contextualize these inflammatory alterations. In order to meet this goal, we have completed the following activities. Tomato growing: Tomatoes (two varieties, OH99-218, high lycopene, and 8614-1-1, low carotenoid) were started from seed in the greenhouse at the Ohio Agricultural Research and Development Center in Wooster, OH in March 2018. Seedlings were planted in the field in May 2018 at the North Central Agricultural Research Station in Fremont, OH. Tomato plants were maintained by project personnel. Tomato harvest: Tomatoes were mechanically harvested (~2500 lbs of each variety). Tomato juice production: Tomatoes were processed from raw fruits into shelf-stable juice at the Ohio State University Food Industries Center pilot plant in Columbus, OH. Tomatoes were processed as previously (Cooperstone et al., 2015), and soy isoflavone extract was added to the high lycopene tomato juice, canned in 6 oz. cans and are being shipped to the USDA Beltsville Human Nutrition Research Center for administering in the human clinical trial. Human clinical trial: Institutional review board (IRB) documents were written and approved at both the Beltsville Human Nutrition Research Center, and Ohio State University, for the execution of this human clinical trial. Subjects recruitment tools were developed, screening occurred, and 12 subjects have completed the duration of our 14 week-long intervention. Additional recruitment has been halted because of COVID-19. We have decided to cease recruitment and focus the remainder of the project on our existing samples. Analysis of tomato-soy juice: After production, the phytochemical analysis of our study agent has been performed. Both individual carotenoids (lycopene, beta-carotene), and soy isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, genistein) have been analyzed using ultra high performance liquid chromatography with phododiode array detection, as previously. Cytokine analysis: We have analyzed inflammatory cytokines using ELISA in all of our subjects at 4 time points (pre tomato-soy 1, post tomato-soy 1, pre low carotenoid control 1, post low carotenoid control 2). We have measured GM-CSF, IFN-gamma, IL1-beta, IL-10, IL-12p40, IL-12p70, IL-13, IL1Ra, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, and TNF-alpha. We have run preliminary statistical analysis to understand differences with each intervention. Using paired t-tests, we have found significant decreases in IL-5 levels after our tomato-soy intervention (as compared to before, P=0.0484). Inflammatory biomarkers: Using CyTOF-MS, we analyzed the immune cell populations of our first 12 subjects. Preliminarily we have found significant differences before and after the tomato-soy intervention in CD19_CD3-B-cells, and CD27-IG-D-naïve B cells (P<0.05 using paired t-tests and a FDR multiple testing correction). Using linear modeling, we found these are the same cell populations with significant changes before and after the tomato-soy intervention. Carotenoid analysis: We have analyzed plasma carotenoids in all subjects at all timepoints. Using paired analysis, we have found that the consumption of our tomato-soy juice increases plasma lycopene (P = 0.002) comparing before and after the intervention. There are significantly higher plasma lycopene levels for subjects after the tomato-soy juice as compared to the low carotenoid intervention (P = 0.004). On average (± standard deviation), plasma lycopene levels were 523 ± 228 nmol/L before intervention, and 1298 ± 665 nmol/L after 4 weeks of tomato-soy juice intervention. When on the low carotenoid tomato juice, average plasma lycopene levels were 721 ± 377 nmol/L before intervention, and 703 ± 434 nmol/L after 4 weeks of intervention. The average increase in lycopene on the tomato-soy intervention was 2.8 fold (range 1.44-5.23). Integration of immune and carotenoid data: To see if changes in significantly affected inflammatory cytokines and cell types are related to carotenoid content, we conducted correlation analysis. We found no significant correlations between IL-5, CD19_CD3-B-cells, and CD27-IG-D-naïve B cells and plasma carotenoid levels. Metabolomic analyses: Urine analysis via untargeted metabolomics revealed distinct metabolic composition after tomato-soy juice. Metabolites derived from soy isoflavones were significantly negatively corelated with IL-5 levels.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Sholola MJ, Miller JL, Bilbrey EA, Torok M, Novotny J, Francis DM, Mace TA, Cooperstone JL. The Effects of a Lycopene-Rich Tomato Juice Enhanced With Soy Isoflavones on Inflammation in Individuals With Obesity. Curr Dev Nutrition 2023:7(1);101489. https://doi.org/10.1016/j.cdnut.2023.101489
|
Progress 03/01/22 to 02/28/23
Outputs
Target Audience:Our target audience of scientists and nutritional professionals were reached with the publication listed below. Sholola MJ, Cooperstone JL. Carotenoid extraction and analysis of blood plasma/serum. Methods in Enzymology 670: Carotenoid and Apocarotenoid Analysis. Ed. Eleanore Wurtzel. In press, 2022. https://doi.org/10.1016/bs.mie.2022.03.021 Changes/Problems:Becuase of COVID and other project delays, we are requestinga NCE. What opportunities for training and professional development has the project provided?This grant has provided training opportunities for a graduate student and technician. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?We plan to conduct the soy isoflavone analysis, and complete the untargeted metabolomic analyses. We also plan to prepare reports and write publications.
Impacts
What was accomplished under these goals?
The overarching goal of this project is to understand how particular dietary components from foods can alter markers of inflammation in humans, while monitoring metabolic changes to contextualize these inflammatory alterations. In order to meet this goal, we have completed the following activities. Tomato growing: Tomatoes (two varieties, OH99-218, high lycopene, and 8614-1-1, low carotenoid) were started from seed in the greenhouse at the Ohio Agricultural Research and Development Center in Wooster, OH in March 2018. Seedlings were planted in the field in May 2018 at the North Central Agricultural Research Station in Fremont, OH. Tomato plants were maintained by project personnel. Tomato harvest: Tomatoes were mechanically harvested (~2500 lbs of each variety). Tomato juice production: Tomatoes were processed from raw fruits into shelf-stable juice at the Ohio State University Food Industries Center pilot plant in Columbus, OH. Tomatoes were processed as previously (Cooperstone et al., 2015), and soy isoflavone extract was added to the high lycopene tomato juice, canned in 6 oz. cans and are being shipped to the USDA Beltsville Human Nutrition Research Center for administering in the human clinical trial. Human clinical trial: Institutional review board (IRB) documents were written and approved at both the Beltsville Human Nutrition Research Center, and Ohio State University, for the execution of this human clinical trial. Subjects recruitment tools were developed, screening occurred, and 12 subjects have completed the duration of our 14 week-long intervention. Additional recruitment has been halted because of COVID-19. We have decided to cease recruitment and focus the remainder of the project on our existing samples. Analysis of tomato-soy juice: After production, the phytochemical analysis of our study agent has been performed. Both individual carotenoids (lycopene, beta-carotene), and soy isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, genistein) have been analyzed using ultra high performance liquid chromatography with phododiode array detection, as previously. High lycopene Low carotenoid tomato-soy juice tomato juice Soy isoflavone Average (mg/day) Average (mg/day) Daidzin 26.9 not detected (nd) Glycitin 17.1 nd Genistin 142.6 nd Daidzein 0.9 nd Glycitein 0.5 nd Genistein 1.9 nd Total 189.9 nd High lycopene Low carotenoid tomato-soy juice tomato juice Carotenoid Average (mg/day) Average (mg/day) Lycopene 53.9 1.5 Beta-carotene 1.8 0.2 Total 55.7 1.7 Cytokine analysis: We have analyzed inflammatory cytokines using ELISA in all of our subjects at 4 time points (pre tomato-soy 1, post tomato-soy 1, pre low carotenoid control 1, post low carotenoid control 2). We have measured GM-CSF, IFN-gamma, IL1-beta, IL-10, IL-12p40, IL-12p70, IL-13, IL1Ra, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, and TNF-alpha. We have run preliminary statistical analysis to understand differences with each intervention. Using paired t-tests, we have found significant decreases in IL-5 levels after our tomato-soy intervention (as compared to before, P=0.0484). Inflammatory method development: We are utilizing an advanced flow cytometry method called mass CyTOF (Cytometry by time of flight) to analyze 41 unique immune surface markers on immune cells from the blood of patients in each of the cohorts. This provides enhanced resolution over traditional flow cytometry techniques. We are staining Peripheral Blood Mononuclear Cells (PBMC) from patient blood samples with antibodies labelled with metals and utilizing mass CyTOF to identify whether we observe changes in immune populations comparing pre and post-diet patient blood draws. Panel of immune populations analyzed from patient PBMC samples using mass CyTOF: Immune Populations Sub-populations CD8+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated CD4+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated B Cells Resting, memory, plasmablasts NK Cells Various subtypes T Regulatory Cells Naïve, Activate, Memory T-helper Cells Th1, Th2, Th17 Monocytes Classical, Non-classical Dendritic Cells Plasmocytoid, Monocytic MDSC Granulocytic, Monocytic Inflammatory biomarkers: Using CyTOF-MS, we analyzed the immune cell populations of our first 12 subjects. Preliminarily we have found significant differences before and after the tomato-soy intervention in CD19_CD3-B-cells, and CD27-IG-D-naïve B cells (P<0.05 using paired t-tests and a FDR multiple testing correction). Using linear modeling, we found these are the same cell populations with significant changes before and after the tomato-soy intervention. Carotenoid analysis: We have analyzed plasma carotenoids in all subjects at all timepoints. Using paired analysis, we have found that the consumption of our tomato-soy juice increases plasma lycopene (P = 0.002) comparing before and after the intervention. There are significantly higher plasma lycopene levels for subjects after the tomato-soy juice as compared to the low carotenoid intervention (P = 0.004). On average (± standard deviation), plasma lycopene levels were 523 ± 228 nmol/L before intervention, and 1298 ± 665 nmol/L after 4 weeks of tomato-soy juice intervention. When on the low carotenoid tomato juice, average plasma lycopene levels were 721 ± 377 nmol/L before intervention, and 703 ± 434 nmol/L after 4 weeks of intervention. The average increase in lycopene on the tomato-soy intervention was 2.8 fold (range 1.44-5.23). Integration of immune and carotenoid data: To see if changes in significantly affected inflammatory cytokines and cell types are related to carotenoid content, we conducted correlation analysis. We found no significant correlations between IL-5, CD19_CD3-B-cells, and CD27-IG-D-naïve B cells and plasma carotenoid levels. Metabolomic analyses: Method development is currently underway for global metabolic profiling of plasma/urine after tomato-soy juice consumption.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2022
Citation:
Sholola MJ, Cooperstone JL. Carotenoid extraction and analysis of blood plasma/serum. Methods in Enzymology 670: Carotenoid and Apocarotenoid Analysis. Ed. Eleanore Wurtzel. 2022;423-457. https://doi.org/10.1016/bs.mie.2022.03.021
|
Progress 03/01/21 to 02/28/22
Outputs
Target Audience:Our target audience of scientists and nutritional professionals were reached with the publication listed below. Sholola MJ, Cooperstone JL. Carotenoid extraction and analysis of blood plasma/serum. Methods in Enzymology 670: Carotenoid and Apocarotenoid Analysis. Ed. Eleanore Wurtzel. In press, 2022. https://doi.org/10.1016/bs.mie.2022.03.021 Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This grant has provided training opportunities for a graduate student and technician. How have the results been disseminated to communities of interest?Results have been disseminated through publications. What do you plan to do during the next reporting period to accomplish the goals?We plan to conduct the soy isoflavone analysis, and complete the untargeted metabolomic analyses. We also plan to prepare reports and write publications.
Impacts
What was accomplished under these goals?
The overarching goal of this project is to understand how particular dietary components from foods can alter markers of inflammation in humans, while monitoring metabolic changes to contextualize these inflammatory alterations. In order to meet this goal, we have completed the following activities. Tomato growing: Tomatoes (two varieties, OH99-218, high lycopene, and 8614-1-1, low carotenoid) were started from seed in the greenhouse at the Ohio Agricultural Research and Development Center in Wooster, OH in March 2018. Seedlings were planted in the field in May 2018 at the North Central Agricultural Research Station in Fremont, OH. Tomato plants were maintained by project personnel. Tomato harvest: Tomatoes were mechanically harvested (~2500 lbs of each variety). Tomato juice production: Tomatoes were processed from raw fruits into shelf-stable juice at the Ohio State University Food Industries Center pilot plant in Columbus, OH. Tomatoes were processed as previously (Cooperstone et al., 2015), and soy isoflavone extract was added to the high lycopene tomato juice, canned in 6 oz. cans and are being shipped to the USDA Beltsville Human Nutrition Research Center for administering in the human clinical trial. Human clinical trial: Institutional review board (IRB) documents were written and approved at both the Beltsville Human Nutrition Research Center, and Ohio State University, for the execution of this human clinical trial. Subjects recruitment tools were developed, screening occurred, and 12 subjects have completed the duration of our 14 week-long intervention. Additional recruitment has been halted because of COVID-19. We have decided to cease recruitment and focus the remainder of the project on our existing samples. Analysis of tomato-soy juice: After production, the phytochemical analysis of our study agent has been performed. Both individual carotenoids (lycopene, beta-carotene), and soy isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, genistein) have been analyzed using ultra high performance liquid chromatography with phododiode array detection, as previously. High lycopene tomato-soy juice Low carotenoid tomato juice Soy isoflavone Average (mg/day) Average (mg/day) Daidzin 26.9 not dected (nd) Glycitin 17.1 nd Genistin 142.6 nd Daidzein 0.9 nd Glycitein 0.5 nd Genistein 1.9 nd Total 189.9 nd High lycopene tomato-soy juice Low carotenoid tomato juice Carotenoid Average (mg/day) Average (mg/day) Lycopene 53.9 1.5 Beta-carotene 1.8 0.2 Total 55.7 1.7 Cytokine analysis: We have analyzed inflammatory cytokines using ELISA in all of our subjects at 4 time points (pre tomato-soy 1, post tomato-soy 1, pre low carotenoid control 1, post low carotenoid control 2). We have measured GM-CSF, IFN-gamma, IL1-beta, IL-10, IL-12p40, IL-12p70, IL-13, IL1Ra, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, and TNF-alpha. We have run preliminary statistical analysis to understand differences with each intervention. Using paired t-tests, we have found significant decreases in IL-5 levels after our tomato-soy intervention (as compared to before, P=0.0484). Inflammatory method development: We are utilizing an advanced flow cytometry method called mass CyTOF (Cytometry by time of flight) to analyze 41 unique immune surface markers on immune cells from the blood of patients in each of the cohorts. This provides enhanced resolution over traditional flow cytometry techniques. We are staining Peripheral Blood Mononuclear Cells (PBMC) from patient blood samples with antibodies labelled with metals and utilizing mass CyTOF to identify whether we observe changes in immune populations comparing pre and post-diet patient blood draws. Panel of immune populations analyzed from patient PBMC samples using mass CyTOF: Immune Populations Sub-populations CD8+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated CD4+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated B Cells Resting, memory, plasmablasts NK Cells Various subtypes T Regulatory Cells Naïve, Activated, Memory T-helper Cells Th1, Th2, Th17 Monocytes Classical, Non-classical Dendritic Cells Plasmocytoid, moncotyic MDSC Granulocytic, Monocytic Inflammatory biomarkers: Using CyTOF-MS, we analyzed the immune cell populations of our first 12 subjects. Preliminarily we have found significant differences before and after the tomato-soy intervention in CD19_CD3-B-cells, and CD27-IG-D-naïve B cells (P<0.05 using paired t-tests and a FDR multiple testing correction). Using linear modeling, we found these are the same cell populations with significant changes before and after the tomato-soy intervention. Carotenoid analysis: We have analyzed plasma carotenoids in all subjects at all timepoints. Using paired analysis, we have found that the consumption of our tomato-soy juice increases plasma lycopene (P = 0.002) comparing before and after the intervention. There are significantly higher plasma lycopene levels for subjects after the tomato-soy juice as compared to the low carotenoid intervention (P = 0.004). On average (± standard deviation), plasma lycopene levels were 523 ± 228 nmol/L before intervention, and 1298 ± 665 nmol/L after 4 weeks of tomato-soy juice intervention. When on the low carotenoid tomato juice, average plasma lycopene levels were 721 ± 377 nmol/L before intervention, and 703 ± 434 nmol/L after 4 weeks of intervention. The average increase in lycopene on the tomato-soy intervention was 2.8 fold (range 1.44-5.23). Integration of immune and carotenoid data: To see if changes in significantly affected inflammatory cytokines and cell types are related to carotenoid content, we conducted correlation analysis. We found no significant correlations between IL-5, CD19_CD3-B-cells, and CD27-IG-D-naïve B cells and plasma carotenoid levels. Metabolomic analyses: Method development is currently underway for global metabolic profiling of plasma/urine after tomato-soy juice consumption.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2022
Citation:
Sholola MJ, Cooperstone JL. Carotenoid extraction and analysis of blood plasma/serum. Methods in Enzymology 670: Carotenoid and Apocarotenoid Analysis. Ed. Eleanore Wurtzel. 2022. https://doi.org/10.1016/bs.mie.2022.03.021
|
Progress 03/01/20 to 02/28/21
Outputs
Target Audience:Our target audience of scientists and nurtitional professionals were reached with the publication listed below. Mukherjee D, DiVincenzo M, Torok M, Choueiry F, Kumar R, Deems A, Miller JL, Hinton A, Geraghty C, Maranon JA, Kulp SK, Coss C, Carson III WE, Hart PA, Cooperstone JL, Mace TA. Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis. Sci Rep, 2020;10:21824. https://doi.org/10.1038/s41598-020-78762-9 Cooperstone JL. Lycopene: Food Sources, Properties, and Effects on Human Health. In: The Handbook of Nutraceuticals and Functional Foods, 3rd edition. Eds. Wildman REC, Bruno RS. CRC Press, Boca Raton, FL, 2020. P.37-53. ISBN 9781498703727 Changes/Problems:Because of government shutdowns and COVID restrictions, the execution of the clinical portion of this proposal encountered a number of problems. In 2021 we decided to halt additional recruitment, and are focusing on the analysis of samples from the subjects who have completed this clinical trial. What opportunities for training and professional development has the project provided?This grant has provided training opportunities for a graduate student,postdoctoral researcher and technician. How have the results been disseminated to communities of interest?Results have been disseminated in peer reviewed publications and in book chapters. What do you plan to do during the next reporting period to accomplish the goals?We plan to conduct the carotenoid analysis, use linear modeling to analyze our cytokine and CyTOF data, and plan to begin the untargeted metabolomic analyses.
Impacts
What was accomplished under these goals?
The overarching goal of this project is to understand how particular dietary components from foods can alter markers of inflammation in humans, while monitoring metabolic changes to contextualize these inflammatory alterations. In order to meet this goal, we have completed the following activities. Tomato growing: Tomatoes (two varieties, OH99-218, high lycopene, and 8614-1-1, low carotenoid) were started from seed in the greenhouse at the Ohio Agricultural Research and Development Center in Wooster, OH in March 2018. Seedlings were planted in the field in May 2018 at the North Central Agricultural Research Station in Fremont, OH. Tomato plants were maintained by project personnel. Tomato harvest: Tomatoes were mechanically harvested (~2500 lbs of each variety). Tomato juice production: Tomatoes were processed from raw fruits into shelf-stable juice at the Ohio State University Food Industries Center pilot plant in Columbus, OH. Tomatoes were processed as previously (Cooperstone et al., 2015), and soy isoflavone extract was added to the high lycopene tomato juice, canned in 6 oz. cans and are being shipped to the USDA Beltsville Human Nutrition Research Center for administering in the human clinical trial. Human clinical trial: Institutional review board (IRB) documents were written and approved at both the Beltsville Human Nutrition Research Center, and Ohio State University, for the execution of this human clinical trial. Subjects recruitment tools were developed, screening occurred, and 12 subjects have completed the duration of our 14 week-long intervention. Additional recruitment has been halted because of COVID-19. We have decided to cease recruitment and focus the remainder of the project on our existing samples. Analysis of tomato-soy juice: After production, the phytochemical analysis of our study agent has been performed. Both individual carotenoids (lycopene, beta-carotene), and soy isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, genistein) have been analyzed using ultra high performance liquid chromatography with phododiode array detection, as previously. High lycopene tomato-soy juice Low carotenoid tomato juice Soy isoflavone Average (mg/day) Average (mg/day) Daidzin 26.9 not dected (nd) Glycitin 17.1 nd Genistin 142.6 nd Daidzein 0.9 nd Glycitein 0.5 nd Genistein 1.9 nd Total 189.9 nd High lycopene tomato-soy juice Low carotenoid tomato juice Carotenoid Average (mg/day) Average (mg/day) Lycopene 53.9 1.5 Beta-carotene 1.8 0.2 Total 55.7 1.7 Cytokine analysis: We have analyzed inflammatory cytokines using ELISA in all of our subjects at 4 time points (pre tomato-soy 1, post tomato-soy 1, pre low carotenoid control 1, post low carotenoid control 2). We have measured GM-CSF, IFN-gamma, IL1-beta, IL-10, IL-12p40, IL-12p70, IL-13, IL1Ra, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, and TNF-alpha. We have run preliminary statistical analysis to understand differences with each intervention. Using paired t-tests, we have found significant decreases in IL-5 levels after our tomato-soy intervention (as compared to before, P=0.0484). Inflammatory method development: We are utilizing an advanced flow cytometry method called mass CyTOF (Cytometry by time of flight) to analyze 41 unique immune surface markers on immune cells from the blood of patients in each of the cohorts. This provides enhanced resolution over traditional flow cytometry techniques. We are staining Peripheral Blood Mononuclear Cells (PBMC) from patient blood samples with antibodies labelled with metals and utilizing mass CyTOF to identify whether we observe changes in immune populations comparing pre and post-diet patient blood draws. Panel of immune populations analyzed from patient PBMC samples using mass CyTOF: Immune Populations Sub-populations CD8+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated CD4+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated B Cells Resting, memory, plasmablasts NK Cells Various subtypes T Regulatory Cells Naïve, Activate, Memory T-helper Cells Th1, Th2, Th17 Monocytes Classical, Non-classical Dendritic Cells Plasmocytoid, moncotyic MDSC Granulocytic, Monocytic Inflammatory biomarkers: Using CyTOF-MS, we analyzed the immune cell populations of our first 12 subjects. Preliminarily we have found significant differences before and after the tomato-soy intervention in CD19_CD3-B-cells, and CD27-IG-D-naïve B cells (P<0.05 using paired t-tests and a FDR multiple testing correction). Metabolomic analyses: Method development is currently underway for global metabolic profiling of plasma/urine after tomato-soy juice consumption.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Mukherjee D, DiVincenzo M, Torok M, Choueiry F, Kumar R, Deems A, Miller JL, Hinton A, Geraghty C, Maranon JA, Kulp SK, Coss C, Carson III WE, Hart PA, Cooperstone JL, Mace TA. Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis. Sci Rep, 2020;10:21824. https://doi.org/10.1038/s41598-020-78762-9
- Type:
Book Chapters
Status:
Published
Year Published:
2020
Citation:
Cooperstone JL. Lycopene: Food Sources, Properties, and Effects on Human Health. In: The Handbook of Nutraceuticals and Functional Foods, 3rd edition. Eds. Wildman REC, Bruno RS. CRC Press, Boca Raton, FL, 2020. P.37-53. ISBN 9781498703727
|
Progress 03/01/19 to 02/29/20
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This grant has provided training opportunities for a graduate student,postdoctoral researcher and technician. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?We plan to finish the clinical trial, complete the inflammatory bioassays, and begin the targeted and untargeted metabolomic analyses.
Impacts
What was accomplished under these goals?
The overarching goal of this project is to understand how particular dietary components from foods can alter markers of inflammation in humans, while monitoring metabolic changes to contextualize these inflammatory alterations. In order to meet this goal, we have completed the following activities. Tomato growing: Tomatoes (two varieties, OH99-218, high lycopene, and 8614-1-1, low carotenoid) were started from seed in the greenhouse at the Ohio Agricultural Research and Development Center in Wooster, OH in March 2018. Seedlings were planted in the field in May 2018 at the North Central Agricultural Research Station in Fremont, OH. Tomato plants were maintained by project personnel. Tomato harvest: Tomatoes were mechanically harvested (~2500 lbs of each variety). Tomato juice production: Tomatoes were processed from raw fruits into shelf-stable juice at the Ohio State University Food Industries Center pilot plant in Columbus, OH. Tomatoes were processed as previously (Cooperstone et al., 2015), and soy isoflavone extract was added to the high lycopene tomato juice, canned in 6 oz. cans and are being shipped to the USDA Beltsville Human Nutrition Research Center for administering in the human clinical trial. Human clinical trial: Institutional review board (IRB) documents were written and approved at both the Beltsville Human Nutrition Research Center, and Ohio State University, for the execution of this human clinical trial. Subjects recruitment tools were developed, screening occurred, and 12 subjects have completed the duration of our 14 week-long intervention. Additional recruitment has been halted because of COVID-19, though the remainder of the subjects we expect to recruit during Summer/Fall 2020. Analysis of tomato-soy juice: After production, the phytochemical analysis of our study agent has been performed. Both individual carotenoids (lycopene, beta-carotene), and soy isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, genistein) have been analyzed using ultra high performance liquid chromatography with phododiode array detection, as previously. Soy isoflavone Average (mg/day) High lycopene tomato-soy juice Low carotenoid tomato juice Daidzin 26.9 nd* Glycitin 17.1 nd Genistin 142.6 nd Daidzein 0.9 nd Glycitein 0.5 nd Genistein 1.9 nd Total 189.9 nd *nd: not detected Carotenoid Average (mg/day) High lycopene tomato-soy juice Low carotenoid tomato juice Lycopene 1.5 53.9 Beta-carotene 0.2 1.8 Total 1.7 55.7 Inflammatory method development: We are utilizing an advanced flow cytometry method called mass CyTOF (Cytometry by time of flight) to analyze over 40 unique immune surface markers on immune cells from the blood of patients in each of the cohorts. This provides enhanced resolution over traditional flow cytometry techniques. We are staining Peripheral Blood Mononuclear Cells (PBMC) from patient blood samples with antibodies labelled with metals and utilizing mass CyTOF to identify whether we observe changes in immune populations comparing pre and post-diet patient blood draws. Panel of immune populations analyzed from patient PBMC samples using mass CyTOF: Immune Populations Sub-populations CD8+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated CD4+ T cells Naïve, Activated, Central Memory, Effector Memory, Terminally-differentiated B Cells Resting, memory, plasmablasts NK Cells Various subtypes T Regulatory Cells Naïve, Activate, Memory T-helper Cells Th1, Th2, Th17 Monocytes Classical, Non-classical Dendritic Cells Plasmocytoid, moncotyic MDSC Granulocytic, Monocytic Inflammatory biomarkers: Using CyTOF-MS, we are in the process of analyzing the immune cell populations of our first 12 subjects. Metabolomic analyses: Method development is currently underway for global metabolic profiling of plasma/urine after tomato-soy juice consumption.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Choueiry F, DiVincenzo M, Deems A, Torok M, Kulp SK, Carson WE, Hart PA, Cooperstone JL, Mace TA. Soy-tomato Enriched Diet Reduces Inflammation and Disease Severity in a Pre-clinical Model of Chronic Pancreatitis. Pancreas 2019;48(10):1414-1415.
|
Progress 03/01/18 to 02/28/19
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This grant has provided professional development and training for graduate students learning crop production, food processing, phytochemical analysis andinflammatory assays. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?During the next period, we plan to complete the human clinical trial, begin the inflammatory assays, carotenoid/isoflavone analyses.
Impacts
What was accomplished under these goals?
The overarching goal of this project is to understand how particular dietary components from foods can alter markers of inflammation in humans, while monitoringmetabolic changes tocontextualize theseinflammatory alterations. In order to meet this goal, we have completed the following activities. Tomato growing: Tomatoes(two varieties, OH99-218, high lycopene, and 8614-1-1, low carotenoid) were started from seed in the greenhouse at the Ohio Agricultural Research and Development Center in Wooster, OH in March 2018. Seedlings were planted in the field in May 2018 at the North Central Agricultural Research Station in Fremont, OH. Tomato plants were maintained by project personnel. Tomato harvest: Tomatoes were mechanically harvested (~2500 lbs of each variety). Tomato juice production: Tomatoes were processed from raw fruits into shelf-stable juice at the Ohio State University Food Industries Center pilot plant in Columbus, OH. Tomatoes were procesed as previously (Cooperstone et al., 2015), and soy isoflavone extract was added to the high lycopene tomato juice, canned in 6 oz. cans and are being shipped to the USDA Beltsville Human Nutrition Research Center for administering in the human clinical trial. Human clinical trial: Instittutional review board (IRB)documents were written and approved for the execution of this human clinical trial. Subjects are being recruited and we expect subjects to begin the study in March 2019.
Publications
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