Source: UNIVERSITY OF GEORGIA submitted to NRP
BUILDING SOIL HEALTH WITH LIVING MULCH CULTIVATION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1014759
Grant No.
2018-67013-27401
Cumulative Award Amt.
$500,000.00
Proposal No.
2017-06178
Multistate No.
(N/A)
Project Start Date
Jan 1, 2018
Project End Date
Dec 31, 2021
Grant Year
2018
Program Code
[A1102]- Foundational Knowledge of Agricultural Production Systems
Recipient Organization
UNIVERSITY OF GEORGIA
200 D.W. BROOKS DR
ATHENS,GA 30602-5016
Performing Department
CROP & SOIL SCIENCES
Non Technical Summary
Living mulch (LM) production utilizes a perennial mulch crop into which row crops are sown. We established a system in which corn is grown in a white clover mulch crop. The system requires minimal N fertilizer, and is in its 3rd year of production with comparisons to other cover crops. Reduced runoff and improved water quality result from the LM system. Preliminary data indicates soil OM dramatically increases in the LM system and GHG emissions may be reduced - both are associated with good soil health. The objectives of this project are to 1) quantify temporal changes of soil quality parameters when using various cover crop systems, 2) quantify the nitrogen supply and crop uptake in corn growing in the LM system as a function of the litter availability and quality, and 3) examine gaseous N and CO2 emissions in the LM and other cover crop systems. Soils have been sampled weekly over the duration of our research, with an archive of 216 samples from which soil health can be measured. pH, CEC, EC, TOC, labile C, N forms, and microbial biomass and communities will be measured in soil samples. BD, H2O infiltration, and aggregate stability will be measured in soils sampled at the end of the growing season (Obj 1). Clover will be labeled with 15N and used in a replacement study to determine recovery in corn and clover (Obj. 2). GHG emissions will be measured in static soil chambers weekly during the growing season and monthly thereafter.
Animal Health Component
40%
Research Effort Categories
Basic
60%
Applied
40%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1020110101030%
2051510101020%
2051649101020%
1320410207030%
Goals / Objectives
The overall objective of this project is to develop a cost-efficient system of corn production that minimizes chemical inputs, promotes environmental sustainability, and restores soil health. This will be accomplished using the following objectives:1) Quantify temporal changes of soil quality parameters when using various annual (crimson clover, cereal rye) or perennial (white clover living mulch) cover crop systems.2) Quantify the N supply and crop uptake in the white clover living mulch system.3) Examine gaseous N and CO2 emissions from the 3 cover crop systems.
Project Methods
Soil samples gathered from plots assigned to: a) a no-till/no cover crop system fertilized with 250 kg N ha-1, a no-till system with (b) crimson clover and (c) cereal rye cover crops fertilized with 100 and 250 kg N ha-1, respectively, and d) the LM system (Figure 5). All plots have been planted for 3 continuous years with six replications, and the surface 15-cm of soil sampled in each plot prior to- and weekly throughout the growing season each year. In total, we have 720 archived soil samples that have been air dried and ground. Selected samples will be used to characterize temporal chemical and physical changes of each treatment.Objective 1. Samples will be analyzed for pH using the CaCl method, CEC, EC, Carlo-Erba total OC and N, permanganate oxidizable C, inorganic NO3 and NH4 and organic N using hot and cold KCl extractions. Physical properties of the cover crop treatments will be assessed at the end of the final growing season. Initial and final soil infiltration rates will be measured at three locations within each plot using a Guelf soil Permeameter. Two soil pits will be dug and horizons delineated into the Ap, BA, Bt1, and Bt2 horizons. Bulk density and wet sieve aggregate stability will be quantified for each horizon. Biological assessments of soil quality will use the same soil samples and will include microbial biomass as measured by chloroform fumigation-extraction of the Ap soil and microbial composition quantified using molecular markers. Composition of microbial communities will be determined by sequencing of metagenomic DNA as per Earth Microbiome Project protocols.Objective 2. A monoculture stand of white clover will be established in October in two 5 x 5 m plots. Galvanized steel flashing will be installed into the soil (10 cm into the soil and 5 cm above the soil). Clover biomass will be cut at the soil surface and removed once symptoms of injury appear (approximately 7 days after application). The second 5 x 5 m plot will be managed similarly but white clover will be fertilized with unlabeled N to be used as control for background levels of 15N. The labeled and unlabeled clover will be used in a field study with 4 treatments (arranged in a randomized complete block with 4 replications) that will be established to estimate 15N uptake by corn and clover, as well as 15N remaining in the soil. The treatments will be as follows: 1) Trt 1 will contain corn grown without any cover crop to serve as a negative control to determine N supply from the soil; 2) Trt 2 will also contain corn without any cover crop but will receive 15N-labeled clover in a 20-cm band centered on the corn row (prior to planting the corn) to determine potential 15N uptake from dead white clover mulch; 3) Trt 3 will contain corn grown with white clover (living mulch); the clover will be sprayed with the herbicide combination (stated above) in 20-cm wide bands, and once herbicides symptoms appear, the aboveground biomass will be removed from the length of the row and weighed; the removed clover from the plot will be replaced with 15N-labeled clover at the same rate. This treatment will provide an estimate of the N supplied by the roots of the killed clover plus the living clover to the side of the row; 4) Trt 4 will be similar to Trt 3, except that the killed clover in 20-cm bands will be replaced with unlabeled clover grown in one of 5 x 5 m plots so that an adequate control is established for background 15N. Corn will be planted in all plots after 15N-labeled and unlabeled clover is placed on the respective treatments. Campbell Scientific Model CS616 reflectometers and Model CS109 temperature probes (Campbell Scientific Inc., Logan, UT) connected to data loggers will be placed at the interface between the killed cover crop residue and the soil to estimate the temperature and water potential of the decomposing residue.Plant and soil samples will be collected weekly after corn planting, and analyzed for total N and 15N contents. A soil subsample will be extracted analyzed for total inorganic N by colorimetry, inorganic N converted to NH4 for 15N analysis by mass spectrometry. Another subsample of soil will be dried at 105oC for 24 and analyzed for total C and total N by dry combustion, and for 15N contents by mass. Plant samples will be dried and analyzed for total C, total N, and 15N. The percent N recovered by the corn and living clover at each sampling date will be calculated using Eq. [1] % N Recovered = [P(c-d)/F(a-b)] x 100, Where P = amount of N in corn or living clover; F = amount of N in labeled clover; c = atom % 15N in living clover or corn; a = atom % 15N in labeled clover; b = atom % 15N in unlabeled clover, and d = atom % 15N in corn or living clover without labeled clover. A similar calculation will be used to estimate the percent of labeled clover N remaining in the soil. Temperature, water potential of the cover crop residue, and soil water content will be used to drive a model that estimates N mineralization from cover crops. This model, which is called The Cover Crop Nitrogen Availability Calculator (available at aesl.ces.uga.edu/mineralization) uses data of cover crop quality and biomass to estimate the timing and amount of nitrogen released. The amount of N released from the killed white clover as measured by the 15N tracer will be compared to the amount of N mineralized estimated by the Cover Crop Nitrogen Availability Calculator to assess model performance.Objective 3. The field plot will consist of 3 treatments: 1) corn grown using the living mulch system, 2) corn grown in a crimson clover cover crop, and 3) corn grown with no cover crop. Soil N2O, NOx, NH3, and CO2 gaseous losses will be measured using a 1 L static chamber system. Chamber collars (10 cm diameter) will be installed into the soil within each plot tillage system in the field plot design. There will be four chamber systems in the plots, including two conventional (tillage and no-till) and two living mulch (within row and between rows). In general, sampling will be on a biweekly basis during the growing season to capture the changes in flux rates in response to the dynamics of the clover/corn interactions, and sampled on a monthly basis during other periods of the year. Emissions will be modeled using teh Community Land Model with prognostic Carbon and Nitrogen (CLM-CN) developed at the National Center for Atmospheric Research (NCAR) with an N2O emissions module (CLMCN-N2O) added. In the current framework, we are only able to simulate N2O emissions from natural soils with no application of nitrogen fertilizers, so we will couple the atmosphere as a climatically active trace constituent within Community Atmosphere Model (CAM)/Whole Atmosphere Community Climate Model (WACCM) to allow for analysis of the two-way impact of soil emissions on climate and stratospheric ozone depletion, and vice versa. Based on the current status of models, we will first implement CLMCN-N2O into the framework of the Community Earth System Model (CESM). This model will be called as DNDC-N2O. At present, greenhouse gases are usually considered as "well-mixed" in the atmosphere, and tracers in CAM are not directly linked to CLM soil emissions. Using the coupled DNDCN2O-WACCM, we will be able to assess the impact of past and present soil N trace gas emissions on atmospheric N2O, NOx, and NH3 mixing ratios, stratospheric ozone destruction, and on UV radiation within CESM. Using WACCM, we will analyze the loss rate of N2O by O(1D), which leads to stratospheric NO production. This will enable us to calculate the current and potential future impacts on stratospheric O3 due to N2O emissions, which will help assess the impact of the land nitrogen cycle on future O3 recovery.

Progress 01/01/18 to 12/11/21

Outputs
Target Audience: Fellow research, extension, NGO, and industry scientists who have a stake in sustainable farming systems. Undergraduate and graduate students Producers who are wanting to decrease their reliance on fertilizer and herbicide inputs. The general public who are concerned about environmentally unsustainable farming techniques. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Training activities: 1) This project trained 3 graduate students: one at Emory University and two at The University of Georgia. Two of the students Emory University and one from The University of Georgia) secured professional appointments in their respective disciplines. One student at the University of Georgia is completing his PhD in agricultural bioinformaticsl. How have the results been disseminated to communities of interest?Results have been disseminated using stakeholder-specific means. These are categorized as: Fellow research, extension, NGO, and industry scientists who have a stake in sustainable farming systems. Research results were published in peer-reviewed journals to reach a general scientific audience. In addition, results were disseminated at National and International professional meetings to provide opportunities for critique, in-depth interaction on specifics of methodologies and where the technology is applicable, and how it will impact future research and outreach efforts. Undergraduate and graduate students. Students were exposed to the theory of the living mulch system in crop and soil sciences classes, followed by field visits and laboratory exercises to illustrate how the living mulch system works, and examine the soil physicaland chemical properties imparted by the living mulch system. Producers who are wanting to decrease their reliance on fertilizer and herbicide inputs. Yearly field days were conducted at the J. Phil Campbell Research and Education Center in which producers could visit the research site, get the most recent research results, and ask questions about their specific requirements for technology transfer and implementation. The general public who are concerned about environmentally unsustainable farming techniques. An annual "Corn Boil" was held at the J. Phil Campbell Research and Education Center in which the public was invited. This is an event in which the public is invited to take tours of the research programs and learn about the reasons for conducting the research,the results of the experiments, and why the experiments are relevant to them.The Corn Boil concluded witha lunch in which hot dogs, homemade baked beans, watermelon, andExperiment Station-grown sweet corn was served. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? 1) Soils in the LM (white clover) system had lower lime buffering capacity and greater pH, base saturation, cation exchange capacity (CEC), Ca, K2O, Mg, P2O5 , and total organic C concentrations than other treatments. Soil NH3 and NO3 had seasonal fluctuations associated with mineral N fertilizer and were lower in the LM treatment. After 3 yr, the soil bulk density was lower and porosity, water infiltration, and labile C were greater in surface soils from the LM treatment than in the surface soils of the other treatments. Use of a perennial LM cover crops expedited soil health regeneration compared to other treatments. 2)The mean total N provided from the cover crops was 145, 105, and 25 kg ha−1 in the LM, Crimson clover (CC), and cereal rye (CR) cover crop systems, respectively. Water uptake by the LM reduced soil water content, soil N availability, N uptake, and plant-available N (PAN) compared with the CC and CR cover crop systems. Average grain yield was 10.4, 13.3, and 13.0 Mg ha−1 for the LM, CC, and CR cover crop systems, respectively. Nitrogen internal utilization efficiency was not different among systems, but fertilizer N partial nutrient balance and partial factor productivity was greatest for LM and least for CR. Overall, the LM system had lower soil N availability, grain yield, and PAN, but it supplied a significant amount of legume N to corn and minimized the need for mineral N. We concluded that success of the LM system is dependent on N mineralization of the white clover residue and that yearly weather variation significantly affects mineralization of cover crop residues and PAN. Using 15N radioisotopes we were able to demonstrate that approximately 160 kg/ha of N was released from the white clover, and greater than 80% of the released nitrogen from the living mulch clover was taken up by the corn. 3) Mean soil CO2 and N2O fluxes (159.7 kg ha−1 d−1 and 0.027 kg N ha−1 d−1, respectively) observed in LM plots exceeded those from other treatments. Soil temperature, moisture, potentially mineralizable nitrogen (N), and nitrate partially explained these differences. Much of the CO2 from the living mulch was associated with plant respiration. Mean soil NH3 emissions were greater in LM (0.089 kg N ha−1 d−1) compared with no cover crop (0.038 kg N ha−1 d−1). Increased N2O and NH3 fluxes could be from release of N from decomposition of clover and from release of N into the soil as the corn shades the clover. Although LM plots did not reduce trace gas emissions, labile carbon content was at least 100 mg kg−1 greater than other treatments after 2 yr, improving soil health.

Publications

  • Type: Journal Articles Status: Other Year Published: 2021 Citation: Hanxia Li, Nicholas Hill, Jason Wallace. 202x. Living mulch fosters a more diverse and balanced bacterial community in corn production.


Progress 01/01/20 to 12/31/20

Outputs
Target Audience:The targeted audiences for this project include professional peers, undergraduate and graduate students, producers, industry stakeholders. Changes/Problems:The major problems we encountered in our research effort were a) weed control during cover crop establishment, and b) perennial weed infestation as age of the LM system increased. Control of annual weeds during establishment was addressed and corrected by using various chemistries of herbicides. Subsequent research is investigating the ecology and control of perennial weeds. What opportunities for training and professional development has the project provided?Two graduate students and two research technicians were assigned to conduct research on this project. Students were required to attend professional meetings (national and regional) and present their results to their professional peers. Project investigators also attended meetings and were able to share the results with peers on formal and informal meetings. How have the results been disseminated to communities of interest?1. Annual field days were conducted where scientists, graduate students, industry and community stakeholders attended. Normal attendance of these annual events ranged between 200 and 300 individuals. 2. Undergraduate and Graduate classes participated in field trips to the research sites to provide an experiential learning opportunity for the student population at UGA. 3. Industry and government (U.S congresspeople; Georgia State Representatives; Georgia Commissioner of Agriculture) stakeholders attended a one-day presentation of ongoing research at the University of Georgia J. Phil Campbell Research and Education Center. 4. Local and industry related news media showcased the living mulch research in print, radio, and television media. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? 1) Soils in the LM (white clover) system had lower lime buffering capacity and greater pH, base saturation, cation exchange capacity (CEC), Ca, K2O, Mg, P2O5, and total organic C concentrations than other treatments. Soil NH3and NO3had seasonal fluctuations associated with mineral N fertilizer and were lower in the LM treatment. After 3yr, the soil bulk density was lower and porosity, water infiltration, and labile C were greater in surface soils from the LM treatment than in the surface soils of the other treatments. Use of a perennial LM cover crops expedited soil health regeneration compared to othertreatments. 2)The mean total N provided from the cover crops was 145, 105, and 25 kg ha−1in the LM, Crimson clover (CC), and cereal rye (CR) cover crop systems, respectively. Water uptake by the LM reduced soil water content, soil N availability, N uptake, and plant-available N (PAN) compared with the CC and CR cover crop systems. Average grain yield was 10.4, 13.3, and 13.0 Mg ha−1for the LM, CC, and CR cover crop systems, respectively. Nitrogen internal utilization efficiency was not different among systems, but fertilizer N partial nutrient balance and partial factor productivity was greatest for LM and least for CR. Overall, the LM system had lower soil N availability, grain yield, and PAN, but it supplied a significant amount of legume N to corn and minimized the need for mineral N. We concluded that success of the LM system is dependent on N mineralization of the white clover residue and that yearly weather variation significantly affects mineralization of cover crop residues and PAN. 3) Mean soil CO2and N2O fluxes (159.7 kg ha−1d−1and 0.027 kg N ha−1d−1, respectively) observed in LM plots exceeded those from other treatments. Soil temperature, moisture, potentially mineralizable nitrogen (N), and nitrate partially explained these differences. Mean soil NH3emissions were greater in LM (0.089 kg N ha−1d−1) compared with no cover crop (0.038 kg N ha−1d−1). Increased N2O and NH3fluxes could be from release of N from decomposition of clover and from release of N into the soil as the corn shades the clover. Although LM plots did not reduce trace gas emissions, labile carbon content was at least 100 mg kg−1greater than other treatments after 2 yr, improving soil health.

Publications

  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Peters, S.J., Saikawa, E., Markewitz, D., Sutter, L., Avramov, A., Sanders, Z.P., Yosen, B., Wakabayashi, K., Martin, G., Andrews, J.S. and Hill, N.S., 2020. Soil trace gas fluxes in living mulch and conventional agricultural systems (Vol. 49, No. 2, pp. 268-280). Hill, N.S., Levi, M., Basinger, N., Thompson, A., Cabrera, M., Wallace, J., Saikawa, E., Avramov, A. and Mullican, J., White clover living mulch enhances soil health versus annual cover crops. Agronomy Journal.doi.org/10.1002/agj2.20768 Basinger, N.T. and Hill, N.S., Establishing white clover (Trifolium repens) as a living mulch: weed control and herbicide tolerance. Weed Technology, pp.1-28. Andrews, J.S., Sanders, Z.P., Cabrera, M.L., Saha, U.K. and Hill, N.S., 2018. Nitrogen dynamics in living mulch and annual cover crop corn production systems. Agronomy Journal, 110(4), pp.1309-1317. Andrews, J.S., Sanders, Z.P., Cabrera, M.L., Hill, N.S. and Radcliffe, D.E., 2020. Simulated nitrate leaching in annually cover cropped and perennial living mulch corn production systems. Journal of Soil and Water Conservation, 75(1), pp.91-102.


Progress 01/01/19 to 12/31/19

Outputs
Target Audience:The living mulch system of producing crops is dependent upon providing conditions conducive for row crops and white clover (the living mulch) to cohabitate. The system is a radical departure from traditional row-crop production and is, therefore, expected to require signicant effort to transfer the technology from the laboratory/experiment stations to the end users. Four distinct audiences were targeted in this past year. First was the scientific community via publications of research results from other living mulch projects to provide background and reasoning for the current research effort. The research findings were published in the Agronomy Journal in an effort to educate peers as to the agronomic and environmental significance of the living mulch project. Presentations were also made to the scientific community at regional and national meetings in order to provide the most up-to-date research findings. The second audience was producers who have demonstrated that they are technology adopters. Inasmuch as row crop production is a novel method of farming, it will require a uniqe set of producers who are willing to try new production systems and follow directions during implementation to achieve the greatest likelihood for success., This was conducted by holding meetings at County extension offices and field days to provide both theory and first-hand experience observing the living mulch system. Producer-targeted field days were designed to explain how management variables will affect how the plant species (clover and row crop) respond to competition from one another when agronomic variables (row spacing, population density, irrigation, etc.) vary. A third targeted audience was graduate and undergraduate students. Our students are well versed in the theoretical aspects of crop production but a diminishing pool are from farm backgrounds. Field trips to the research plots demonstrated the difference between productoin practices when crops are grown in the living mulch system, winter annual cover crops, and no cover crop scenarios. Two undergraduate and two graduate students students worked on the research project and experience the practice first-hand. The fourth targeted audience was citizen groups. These groups have an interest in agriculture and/or the environmental consequences of agricultural practices. A field day was conducted to provide a hands-on experience of the living mulch production system and was complemented with data from the experiments illustrating the benefit to soil health, the ecosystem services, and economic advantage producers. This holistic approach to community education was well received and highlighted in local newspapers. Changes/Problems:none What opportunities for training and professional development has the project provided?Objective 1. One graduate student from the University of Georgia gathered and conducgted water infiltration measurements, ran SOM and POX analyses on the soil samples, and assisted with statistical analysis.. Objective 2. One graduate student from the University of Georgia conducted this experiment, including labeling clover with 15N, harvesting and applying labeled clover to research plots, monitoring soil and clover residue moisture, and sampling clover and corn tissues, quantifiying corn and clover biomass, sampling soils, and preparing samples for analysis. Objective 3. One graduate student from Emory University (Atlanta) assumed leadership on making soil gaseous measurements. A second graduate student from the University of Georgia provided logistical assistance. How have the results been disseminated to communities of interest?Both students presented research findings from objectives 2 and 3 at professional meetings. The PI from the University of Georgia presented the findings from Objective 1 to one international scientific meeting and one national scientific meeting. Four field day events were held at two locations in Georgiaand one location in Alabama to disseminate the research findings to rural and urban stakeholders. What do you plan to do during the next reporting period to accomplish the goals?Objective 1. Submit a research article on the effect of cover crops and physical and chemical properties of soil (likely to Agronomy J. or J.SSSA). Objective 2. Complete 15N siotopic analysis of samples and conduct statistical analysis on all data for this objective. Objective 3. Finish carbon equivalent calculations for each cover crop system.; Model potential impact of each cover crop production system on global warming.

Impacts
What was accomplished under these goals? Objective 1. Analysis of variance indicated there were significant main treatment effects (cover crop, sampling date within year, and year) differences on most soil chemical attributes. Cover crops affected temporal changes in soil chemical composition for lime buffering capacity, pH, base saturation, CEC, Ca, K, Mg, P, NH3, NO3, total organic carbon (TOC), total soil N, and labile C, but not Fe or soluble salts. Generally speaking, pH, base saturation, CEC, Ca, K, Mg, P, TOC, and total N were greater among the legume cover crop treatments (crimson clover and white clover) than treatments of no cover crop or cereal rye. Lime buffering capacity was greatest when no cover crop was used but not different among the other cover crop treatments. There were interactions among cover crop and sampling date treatments for NH3 and NO3, and between cover crop and year for total organic carbon. Soil NH3 and NO3 was greatest after top dressing with mineral N in the no cover crop and cereal rye treatments, but were relatively unchanged regardless of sampling date in the crimson clover and white clover treatments. Soil NO3 was not different regardless of cover crop treatment early in the growing season, but was least in the white clover cover crop treatment during mid-season. There were no differences in soil NO3 among cover crop treatments later in the growing season. Total organic carbon in the soil was greatest in the white clover cover crop treatment in all years except 2016, when drought occurred. There were no differences in TOC among cover crop treatments in 2016. TOC in the white cover treatments increased as years progressed, but there were no differences in TOC from 2015 to 2018 in the other cover crop treatments. Bulk density decreased in treatments using white clover as a cover crop. Soil bulk density was not different among the other cover crop treatments. Porosity and saturated surface water infiltration rates increased in the white clover cover crop treatment but was not different among the other cover crop treatments. Soil water holding capacity was not affected by cover crop treatment, indicating that increased porosity in the white clover treatment was due to macropores. Objective 2. Total decomposition of the white clover residue within herbicide treated strips and release of 15N labeled N from the residue occurred within 60 days of planting the corn in 2018. The labeled N was taken up (primarily) by seedling corn, with minimal labeled N found in the living (and competing white clover) or in the soil. Labeled N content of the corn was inversely related to the labeled N remaining in the decomposing clover (ie. as N was released from the residue it was taken up by the corn). Approximately 97% of the labeled N was found in the corn at the end of the growing season. The data suggest approximately 33 of the 35 kg/ha of N released from herbicide treated clover strips is available and taken up by seedling corn. A complementary study investigating N rates applied as starter fertilizer indicated no yield differences between 20 or 40 kg/ha N was added at planting. The experiments combined indicate that 20 kg/ha starter N was sufficient to meet the recommended 50 kg/ha N at corn planting. This experiment was repeated in 2019 but isotopic analysis of plant and soil samples has not been completed at the time this report was written. Objective 3. Total soil CO2 emission was greatest in the white cover treatment. About 40% of the CO2 emission was autotrophic from white clover root respiration. Still, the amount of CO2 emission from the white clover treatments was greater than the other cover crop treatments, likely due to increased labile C in the soil. NOx and NH3 gas emission was slightly greater in the white clover treatment than in other cover crops. A total carbon equivalent assessment is being conducted to determine the impact of drastically reduced mineral N use vs. the slightly higher soil GHG emissions.

Publications


    Progress 01/01/18 to 12/31/18

    Outputs
    Target Audience:The living mulch system of producing crops is dependent upon providing conditions conducivefor row crops and white clover (the living mulch) to cohabitate. The system is a radical departure from traditional row-crop production and is, therefore, expected to require signicant effort to transfer the technology from the laboratory/experiment stations to the end users. Four distinct audiences were targeted in this past year. First was the scientific community via publications of research results from other living mulch projects to provide background and reasoning for the current research effort. The research findings were published in the Agronomy Journal in an effort to educate peers as to the agronomic and environmental significance of the living mulch project. Presentations were also made to the scientific community at regional and national meetings in order to provide the most up-to-date research findings. The second audience was producers who have demonstrated that they are technology adopters. Inasmuch as row crop production is a novel method of farming, it will require a uniqe set of producers who are willing to try new production systemsand follow directions during implementation to achieve the greatest likelihood for success., This was conducted by holding meetings at County extension offices and field days to provide both theory and first-hand experience observing the living mulch system. Producer-targeted field days were designed to explain how management variables will affect how the plant species (clover and row crop)respond to competition from one another when agronomic variables (row spacing, population density, irrigation, etc.) vary. A third targeted audience was graduate and undergraduate students. Our students are well versed in the theoretical aspects of crop production but a diminishing pool are from farm backgrounds. Field trips to the research plots demonstrated the difference between productoin practices when crops are grown in the living mulch system, winter annual cover crops, and no cover crop scenarios. Two undergraduate and two graduate students studentsworked on the research projectand experience the practice first-hand. The fourth targeted audience was citizen groups. These groups have an interest in agriculture and/or the environmental consequences of agricultural practices. A field day was conducted to provide a hands-on experience of the living mulch production system and was complemented with data from the experiments illustrating the benefit to soil health, the ecosystem services, and economic advantage producers. This holistic approach to community education was well received and highlighted in local newspapers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Objective 1. One graduate student from the University of Georgia gatheredand conducgted water infiltration measurements, ran SOM and POXanalyses on the soil samples, and assisted with statistical analysis.. Objective 2. One graduate student from the University of Georgia conducted this experiment, including labeling clover with 15N, harvesting and applying labeled clover to research plots, monitoring soil and clover residue moisture, and sampling clover and corn tissues, quantifiying corn and clover biomass, sampling soils, and preparing samples for analysis. Objective 3. One graduate student from Emory University (Atlanta) assumed leadership on making soil gaseous measurements. A second graduate student from the University of Georgia provided logistical assistance. Visiting and prospective graduate students from China, South Korea, and the United States visited the research plots on a regular basis. How have the results been disseminated to communities of interest?Updated information was presented at the American Society of Agronomy National meetings held in Baltimore, MD on November 4, 2018. What do you plan to do during the next reporting period to accomplish the goals?Objective 1. We will finish analyzing the soil samples for aforementioned response variables and conduct statistical analyes for each. Objective 2. Statistical analysis we be conducted on recovery of 15N in corn and clover to determine the competitiveness of each for minearalized clover N. The experiment will be repeated in thesummer of 2019 to validate the results from 2018. Objective 3. Soil gaseous emission data will be used to model the potential impact of each cover crop production system on global warming.

    Impacts
    What was accomplished under these goals? Objective 1.A fourth year of continuous production of corn in the white clover living mulch (LM), cereal rye, crimson clover, and no cover crop systems was completed. Soils samples taken at the v4, v12, and R1 stages of corn development were sorted. Organic matter (ignition=OM) and permanganate oxidizable carbon (POX) were quantified in each sample There were significant cover crop treatment effects for both OM and POX with the living mulch being the greatest for both response variables and the no cover crop the least for each. Crimson clover had less POX than LMbut was greater than cereal rye. Crimson and LM had similar OM, but both were greater than cereal rye, which was greater than the no cover crop treatment. Bulk density and water infiltration was greatest for LM, follwed by cereal rye and crimson clover, and least for the no cover crop treatment. All soil samples are currently being analyzed for N, P, K, pH, CEC, EC, SOC, SON, and mineral N. Objective 2. Competition between corn and clover for N released in the LM system was tested using a 15N study. A stand of white clover was labeled using 15N ammonium sulfate applied in granular form 1 month prior to harvest. Harvested clover was used to either replace clover in the LM system, placed as a mulch layer over the row of corn in a no-cover crop plot, and compared to unlabeled systems of each treatment. Corn and clover samples were gathered on a weekly basis until all clover residue had been mineralized. Dry weights of each were recorded. Samples were submitted to the UGA isotopic analytical lab for analysis and results are expected in the near future. Objective 3. Soil gasseous N and CO2 emissions were measured using a Picarro in-field gas analyzer. Three chambers were placed in each plot and measured using both open and closed chamber methods weekly beginning in March and ending in August, 2018. Data are in the process of being analyzed and interpreted.

    Publications

    • Type: Journal Articles Status: Accepted Year Published: 2018 Citation: Sanders, Z.P. J.S. Andrews, and N.S. Hill. 2018. Water use efficiency in living mulch and annual cover crop corn production systems. Agron. J. 110:1128-1135. 91. Andrews, J.S., Z.P. Sanders, M. Cabrera, U.K. Saha, and N.S. Hill. 2018. Nitrogen dynamics in living mulch and annual cover crop corn production systems. Agron. J. 110:1309-1317.