Progress 10/01/19 to 09/30/20
Outputs
Target Audience:The target audiences for our work include scientists in multiple fields related to crop protection (particularly entomology, nematology, plant pathology, and plant molecular biology, as well as the general public, who wereachthrough K12 educational efforts. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This year, the project provided research opportunities for 2 undergraduate Honors students,one M.S. student,one Ph.D. student, and two postdoctoral associates who contributed to the project on a part-time basis. Over the course of the year,the postdoctoral associates and tgraduate students had opportunities to give scientific presentations, mentor more junior scientists in the laboratory, and participate in K12 outreach; most were also engaged in the process of preparing manuscripts and grant applications for peer review. The Ph.D. student on the project also received the2020 Dale Bumpers Distinguished PhD Scholar Award from the Dale Bumpers College of Agricultural, Food and Life Sciences, University of Arkansas. This award is the highest recognition for a graduate student within the college, and is granted to one Ph.D. student within the college per year. How have the results been disseminated to communities of interest?This year, we published two peer-reviewed papers and one preprint, and submitted 3additional manuscripts and a patent application. Opportunities for conference presentations and outreach events were severely limited this year due to COVID19, but we were able to give two conference talks prior to the pandemic. What do you plan to do during the next reporting period to accomplish the goals?Towards objective 1, in the coming year we will be characterizing how nematode infection levels on soybean are impacted by plant elicitor peptide and propeptide levels. Towards objective 2, we will continue to characterize the influence of aphid infestation on reactiveoxygen (ROS) accumulation, and the impacts of ROS on host suitability. Towards objective 3, we will continue to investigate the impacts of fatty acid metabolism on ROS accumulation, photosynthesis, andhost suitability for insects. To further the educational mission of the project, the principal investigator will continue to mentor two graduate students and two undergraduate students.In addition, we will pursue funding for improved bioimaging infrastructure to support this project.
Impacts
What was accomplished under these goals?
In 2020, my laboratory has made significant advances in studying the role of singlet oxygen in plant-insect interactionsand investigating the influence of membrane lipid composition in plants on insect resistance. Singlet oxygen is an extremely potent and short-lived reactive oxygen species generated primarily in the chloroplast as a byproduct of photosynthesis and also in response to stress. Perhaps because of the technical challenges in detecting singlet oxygen, relatively few laboratories world-wide have examined its potential involvement in plant responses to biotic stress. Through time-consuming genetic crosses with various mutants and transgenic lines expressing a singlet oxygen-responsive reporter gene, my laboratory has developed a set of plant materials that allow us to investigate the potential roles of singlet oxygen in plant response to aphids or other stresses. Using these materials, we have demonstrated that a) aphid infestation induces an oxidative response in the chloroplast and increased singlet oxygen accumulation; b) artificial induction of singlet oxygen in plants reduces aphid infestations; c) base-line singlet oxygen levels are heightened in an aphid-resistant mutant plant line that has altered chloroplast membrane composition; and d) genes that are required for signaling in response to singlet oxygen contribute to aphid resistance in plants with altered chloroplast membrane composition. Cumulatively, our data indicate that aphid resistance in plants can be enhanced by manipulating the chloroplast lipid composition, and that this enhanced aphid resistance involves enhanced singlet oxygen-dependent defense signaling. Surprisingly, we found that aphid-resistant plants with modified chloroplast lipid composition also have enhanced photosynthetic efficiency; thus, manipulation of chloroplast lipid composition may have benefits to plant productivity as well as to aphid resistance. These results represent an exciting breakthrough in our understanding of the roles of reactive oxygen species and the chloroplast in plant defense, and they also shed light on the impacts of membrane lipids on plant immunity and plant productivity. We believe this work will have important implications for crop protection and improvement. We have reported portions of these findings in a publication in Frontiers in Plant Science and in manuscripts in review at Molecular Plant Pathology and Molecular Plant Microbe Interactions; we expect to develop at least one additional publication on these results in 2021.
Publications
- Type:
Journal Articles
Status:
Submitted
Year Published:
2021
Citation:
McInturf, S., Khan, M., Gokul, A., Castro-Guerrero, N. A., Hoehner, R., Li, J., Kunz, H. H.,
Goggin, F., Keyster, M., Mendoza-Cozatl, D. Cadmium interference with iron sensing reveals transcriptional programs sensitive and insensitive to reactive oxygen species. Submitted to Journal of Experimental Botany.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Goggin, F., Roper, D. K. "Materials for Agricultural Resource Imaging Analytics at High Resolution (MARIAH)," Center for Advanced Surface Engineering NSF EPSCoR Annual Meeting, Petit Jean, AR, United States. (January 2020). Invited oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Goggin, F. "Working with the NSF: The Merit Review Process, Collaborative Research & Large Center Grant Opportunities and Responsibilities," Center for Advanced Surface Engineering NSF EPSCoR Annual Meeting, Petit Jean, AR, United States. (January 2020). Served as an invited panel member for a group discussion of best practices to obtain large collaborative grants.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Lee, M. W., Padilla, C. S., Gupta, C., Galla, A., Pereira, A., Li, J., Goggin, F. (2020). The FATTY ACID DESATURASE2 Family in Tomato Contributes to Primary Metabolism and Stress Responses. Plant Physiology, 182 (2), 1083-1099.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2021
Citation:
Xu, J. L., Padilla, C. S., Li, J. L., Wickramanayake, J. L., Goggin, F. Redox responses of Arabidopsis thaliana to the green peach aphid, Myzus persicae. Molecular Plant Pathology.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2021
Citation:
Li, J. L., Galla, A. L., Avila, C. A., Flattman, K. L., Vaughn, K., Goggin, F. Fatty Acid Desaturases in the chloroplast and endoplasmic reticulum promote susceptibility to the Green Peach Aphid, Myzus persicae, in Arabidopsis thaliana. Molecular Plant Microbe Interactions.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
McInturf, S., Khan, M., Gokul, A., Castro-Guerrero, N. A., Hoehner, R., Li, J., Kunz, H. H., Goggin, F., Keyster, M., Mendoza-Cozatl, D. Cadmium interference with iron sensing revealstranscriptional programs sensitive and insensitive to reactive oxygen species. BioR?iv. https://www.biorxiv.org/content/10.1101/2020.07.05.188649v1.full
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Wickramanayake, J., Goss, J., Zou, M., Goggin, F. (2020). Loss of function of FATTY ACID DESATURASE 7 in tomato enhances photosynthetic carbon fixation efficiency. Frontiers in Plant Science, 11, Article 932.
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Progress 10/01/18 to 09/30/19
Outputs
Target Audience:The target audiences for our work include scientists in multiple fields related to crop protection (particularly entomology, nematology, plant pathology, and plant molecular biology), as well as the general public, who were reached through K12 educational efforts. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? This year,2 new undergraduate Honors studentsbegan working on the project. Withmentoringfrom the principal investigator and a graduate student, they each submitted research proposals to the NSF Summer Undergraduate Research Fellowship (SURF) program (both selected for funding by the University of Arkansas Honors College). The project also provided research opportunities for one new M.S. student, onecontinuing Ph.D. student, and threepostdoctoral associates who contributed to the project on a part-time basis. Over the course of the year, all of the postdoctoral associates and the Ph.D. studenthad opportunities to give scientific presentations, mentor more junior scientists in the laboratory, and participate in K12 outreach; most were also engaged in the process of preparing manuscripts and grant applications for peer review.The Ph.D. student on the project also competed for and won several local, regional, and national awards totaling over$19,000, including the Philanthropic Education Organization (P.E.O.) Scholar Award and the Entomological Society of America'sLillian and Alex Feir Graduate Student Travel Award in Insect Physiology, Biochemistry, or Molecular Biology. How have the results been disseminated to communities of interest?This year, we published one peer-reviewed paper, prepared 2 additional manuscripts for submission, submitted an invention disclosure, and gave 15 presentations at professional conferences or seminar series. The principal investigator also organized and moderated a symposium at the annual meeting of the Entomological Society of America and co-organized a workshop at the American Society of Plant Biology's annual Phenome meeting. In addition, the project leader, students, and staffalso communicated our workto the general public through a variety of outreach events including the 2018Arkansas Insect Festival (attended by~1,900 elementary school students, teachers, and parents), aGirl Scout Field Trip(~25 students), aSummer Nature Camp for the Northwest ArkansasEmergency Children's Shelter, (~15 participants), and theStroll the Atolls Festival, an event forthe local Marshallese community (attended by several hundred people). What do you plan to do during the next reporting period to accomplish the goals?Towards objective 1, in the coming year we will be characterizing nematode infection levels on transgenic soybean lines that overexpress plant elicitor peptides to activate plant defense response. Towards objective 2, we will characterizehow manipulation of singlet oxgen accumulation (through suppression or enhancement of this ROS) impacts herbivore attack. Towards objective 3, we will continue to investigate the impacts of fatty acid metabolism and photosynthetic rates on levels of plant defense against phloem-feeding insects. To further the educational mission of the project,the principal investigator will continue to mentor two graduate students and two undergraduate students.
Impacts
What was accomplished under these goals?
A major focus of the project this year has been the interaction between plant defense and photosynthesis, and we have established that a form of aphid resistance in tomato is associated with improved photosynthetic activity and increased vegetative growth. Carbon assimilation, a measure of net photosynthetic activity, is higher in the aphid-resistant tomato genotype compared to nearly isogenic controls, both in the presence and absence of aphids. Resistant plants also have higher levels of Rubisco, the first enzyme required for carbon fixation. Increased carbon assimilation in the resistant plants is due to increased efficiency of multiple steps in the photosynthetic pathway, including enhanced electron transport, Rubisco activity, and recycling of the substrate for Rubisco, RuBP. We hypothesize that these enhancements are due to changes in the abundance and/or morphology of the chloroplasts, and we have initiated several new approaches to testthis hypothesis, including applying flow cytometry and fluorescence microscopy to characterize chloroplast morphology. We are also characterizing levels of reactive oxygen species (ROS) in the chloroplasts of resistant and susceptible plants to determine if ROS may link photosynthetic activity with aphid resistance. This work is significant because it has identified a novel source of enhanced photosynthesis, and demonstrated that insect resistance can in some cases be synergistic with rather than competitive with plant growth.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Lee M., Huffaker A., Crippen D., Robbins R.T., and Goggin F.L. 2018. Plant Elicitor Peptides Promote Plant Defenses against Nematodes in Soybean. Molecular Plant Pathology, DOI: 10.1111/mpp.12570.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2020
Citation:
Wickramanayake J., Goss J., Zou M., Goggin F.L. Loss of function of FATTY ACID DESATURASE 7 in tomato enhances photosynthetic efficiency. In review.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2020
Citation:
McInturf S., Khan M., Gokul A., Castro-Guerrero N.A., Hoehner R., Li J., Kunz H.H., Goggin F.L., Keyster M., Mendoza-Cozatl D. Cadmium interference with iron sensing reveals transcriptional programs sensitive and insensitive to reactive oxygen species.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Goggin, F.L. 2018. Influence of Fatty Acid Metabolism on Plant-aphid Interactions. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting, November 11-14, Vancouver, BC, Canada. Research talk for symposium (International).
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Fischer, H., Xu, J., Alnasrawi, A., and Goggin, F.L. 2018. Singlet oxygen for defense against aphids. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting, November 11-14, Vancouver, BC, Canada. Symposium poster (International).
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Goggin, F.L. 2018. Plant Metabolism in Plant-Herbivore Interactions: Crossing the Borders between Primary and Secondary Metabolism. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting, November 11-14, Vancouver, BC, Canada. Introductory remarks for symposium (International).
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Goggin, F. 2019. Molecular determinants of host plant resistance to phloem-feeding herbivores. University of Georgia Department of Entomology Seminar, May 2019, Athens GA. (Seminar)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Goggin, F. 2019. Impacts of Plant Immunity and Primary Metabolism on Aphid Infestation. STEM Seminar Series, College of Science, Technology, Engineering, and Mathematics, University of Arkansas, Fort Smith, September 2019, Fort Smith, AR. (Seminar)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Wickramanayake, J., and Goggin, F.L. 2018. Comparison of Photosynthetic Activity in Tomato Genotypes with Differing Levels of Fatty Acid Desaturation and Aphid Resistance. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting, November 11-14, Vancouver, BC, Canada. Symposium poster (International).
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Fischer, H., Xu, J., and Goggin, F.L. 2018. Singlet oxygen as a signaling molecule for aphid resistance. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting, November 11-14, Vancouver, BC, Canada. Oral presentation. (International).
- Type:
Conference Papers and Presentations
Status:
Under Review
Year Published:
2019
Citation:
Goggin, F., Lorence A., McClure G., Stanley S., Walker J., Jurisson S., Braun D., Tai Y. 2019. Plant Imaging Consortium. Arkansas Bioinformatics Conference (AR-BIC), February 2019, Little Rock, AR.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Goggin F.L, and Lawrence-Dill C. 2019. NSF Engineering Research Center Planning Grant: Materials for Agriculture Resource Imaging Analytics at High Resolution (MARIAH). American Society of Plant Biologists Phenome 2019 Conference, February 6-9, Tuscon, AZ.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Wickramanayake, J., and Goggin, F.L. 2019. Phenotyping photosynthetic activity in tomato genotypes with varying levels of foliar fatty acid desaturation. American Society of Plant Biologists Phenome 2019 Conference, February 6-9, Tuscon, AZ. Refereed poster presentation, presented by F.G.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Roper D.K., Castellano M., Clarke J.L., Li C., Liang C.L., Reeves C., Lawrence-Dill C., Lorence A., Dong L., and Goggin F.L. 2019. Engineering Research Center for Materials for Agriculture Resource Imaging Analytics at High Resolution (MARIAH). American Society of Plant Biologists Phenome 2019 Conference, February 6-9, Tuscon, AZ. Refereed poster presentation, presented by F.G.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Fischer, H., and Goggin, F. 2019. Reactive oxygen species as a plant defense mechanism against aphids. Southeastern Branch Entomology Society of America Annual Meeting, March 3-9, Mobile, AL. Oral presentation by H.F., a Ph.D. student in the Goggin lab.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Wickramanayake, J., Goss, J., Zou, M., and Goggin, F.L. 2019. Comparisons of leaf characteristics in tomato genotypes with different photosynthetic efficiencies. Arkansas NSF EPSCoR Annual Conference, May 15-16, Little Rock, AR. Poster presentation, presented by J.W., a member of the Goggin lab.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Wickramanayake, J., and Goggin, F.L. 2019. Comparison of photosynthetic activity in tomato genotypes with differing levels of fatty acid desaturation and aphid resistance. University of Missouri Interdisciplinary Plant Group (IPG) Symposium Plant Signaling in Biotic and Abiotic Stress, May 29-31, Columbia, MO. Poster presentation, presented by J.W., a member of the Goggin lab.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Wickramanayake, J., and Goggin, F.L. 2019. Comparison of photosynthetic activity in tomato genotypes with differing levels of fatty acid desaturation and aphid resistance. American Society of Plant Biology Annual Meeting, August 3-7, San Jose, CA. Poster presentation presented by J.W., a member of the Goggin lab.
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Progress 11/28/17 to 09/30/18
Outputs
Target Audience:The target audience for this project consists of entomologists, nematologists, and other plant scientists, including breeders. The results have also been communicated to the general public through outreach events. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Through this project, one undergrduate intern (Emily Asbeck, recruited through the INBRE summer internsip program) received training in DNA extraction, PCR, aphid bioassays, statistics, and scientific presentation). One Ph.D. student completed herdegree (Janithri Wickramanayake), and another current student (Hillary Fischer) received reserach training had the opportunity to present her research at the national Entomological Society of America's annual meeting, as well as local and regional meetings. All three trainees also participated in K12 outreach events to gain experience with teaching. How have the results been disseminated to communities of interest?This year, we published one peer-reviewed paper, prepared 2 additional manuscripts for submission, and presented our work at ten professional conferences orseminar series. INVITED PRESENTATIONS: Goggin, F.L. 2018. Genomic & Phenomic Approaches to Study Herbivore Resistance in Plants. Department of Entomology and Nematology Departmental Seminar, University of California, Davis, CA, Jan. 2018. Goggin, F.L. 2018. Genomic & Phenomic Approaches to Study Herbivore Resistance in Plants. Department of Entomology and Plant Pathology Departmental Seminar, North Carolina State University, Raleigh, NC, Sept. 2018. Wickramanayake J.S. and F.L. Goggin. Influence of fatty acid desaturation on photosynthetic parameters and aphid resistance in tomato. Oklahoma State University - Department of Entomology and Plant Pathology, Stillwater, OK. September 2017 (invited departmental seminar; JW was the speaker). SUBMITTED PRESENTATIONS: Galla, A., Li, J., and Goggin, F.L. 2018. Influence of ω-3 Fatty Acid Desaturases in the Chloroplast and Endoplasmic Reticulum on Plant-Aphid Interactions. Plant Biology 2018, A joint meeting of the American Society of Plant Biologists, Canadian Society of Plant Biologists, and the International Society of Photosynthesis Research. July 2018, Montreal, Canada. Refereed poster (International. 1st and 2nd author = postdocs in Goggin lab). Asbeck, E., Galla, A., and Goggin, F.L. 2018. The PR1 gene in Arabidopsis thaliana contributes to plant defenses against the Green Peach Aphid, Myzus persicae. IDeA Networks of Biomedical Research Excellence (INBRE) Undergraduate Presentation Session, July 2018, Fayetteville AR. (1st author = summer intern in Goggin lab). Fischer, H.and Goggin, F.L. 2018. The role of singlet oxygen in aphid-resistantFatty Acid Desaturase7Arabidopsis mutants. Southeastern Branch Entomological Society of America, Orlando, FL. March 4-6, 2018. Oral presentation. Fischer, H.and Goggin, F.L. 2018. The role of singlet oxygen in aphid-resistantFatty Acid Desaturase7Arabidopsis mutants. Arkansas Entomological Society Annual Meeting, Fayetteville, AR. February 19, 2018. Oral presentation. Fischer, H.D. and Goggin, F.L.Elucidating the mechanism of aphid resistance inFatty Acid Desaturase 7Arabidopsismutants. Arkansas Crop Protection Association Conference, Fayetteville, AR. November 2017 (oral presentation). Fischer, H.D. and Goggin, F.L.Elucidating the mechanism of aphid resistance inFatty Acid Desaturase 7Arabidopsismutants. Entomological Society of America Annual Meeting, Denver, CO. November 2017 (oral presentation). 2nd Place. Wickramanayake J.S. and F.L. Goggin. Influence of fatty acid desaturation on photosynthetic parameters and aphid resistance in tomato. Entomological Society of America Annual Meeting, Denver, CO. November 2017 (oral presentation). What do you plan to do during the next reporting period to accomplish the goals?A major focus of our work in the coming year will be to address objective 2, the role of reactive oxygen species (ROS)in plant-aphid interactions. We will utilize reporter genes associated with specific ROSor targeted to specificsub-cellular compartments to assessthe identity, timing, and localization of ROS accumulation in response to aphid infestation. We will also utilize mutant lines with modified antioxidant content to evaluate how manipulation of ROS accumulation influences levels of resistance or susceptibility to aphids.
Impacts
What was accomplished under these goals?
In 2018, as part of our investigation of the impact of plant metabolism on aphids, we identified several genes that contribute to plant defenses against aphids, including the Hydroperoxide Lyase (HPL) gene. HPL helps plants prevent aphids from settling on and feeding on tomato leaves. Interestingly, we found that aphid resistance in tomato is unaffected by Lipoxygenase C (LOXC), another gene that works together with HPL to shape the smell and taste of tomato fruits. Potentially HPL may impact aphids through LOX-independent pathway(s). These conclusions are based on our work in two genetic backgrounds, wild type tomato (Solanum lycopersicumcv. Castlemart), and an isogenic mutant line, suppressor of prosystemin-mediated responses2 (spr2). The spr2 mutation in tomato, which disrupts function of Fatty Acid Desaturase 7 (FAD7), confers resistance to the potato aphid (Macrosiphum euphorbiae) and modifies the plant's C6 volatile profiles. To investigate whether C6 volatiles play a role in resistance, Hydroperoxide Lyase (HPL), which encodes a critical enzyme in C6 volatile synthesis, was silenced in wild-type tomato plants and spr2 mutants. Silencing HPL in wild-type tomato increased potato aphid host preference and reproduction on five-week old plants, but had no influence on 3-week old plants, suggesting that the effects of HPL on aphids are age-dependent. The spr2 mutation in contrast conferred strong aphid resistance at both 3- and 5-weeks, and silencing HPL in spr2 did not compromise this aphid resistance. Moreover, a homologous mutation in Arabidopsis thaliana (fad7-1) also conferred resistance to the green peach aphid (Myzus persicae) in a genetic background that carries a null mutation in HPL. These results indicate that HPL contributes to certain forms of aphid resistance in tomato, but that the effects of FAD7 on aphids in tomato and Arabidopsis are distinct from and independent of HPL.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Li, J., Avila, C.A., Tieman, D.M., Klee, H.J., and Goggin, F.L. 2018. A Comparison of the Effects of Fatty Acid Desaturase 7 and Hydroperoxide Lyase on Plant-Aphid Interactions. International Journal of Molecular Science, Apr 4;19(4). pii: E1077. doi: 10.3390/ijms19041077.
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