Source: LOUISIANA STATE UNIVERSITY submitted to NRP
DEVELOPMENT OF A TOXIC BAIT FOR FERAL SWINE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1014218
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Nov 1, 2017
Project End Date
Oct 31, 2021
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
LOUISIANA STATE UNIVERSITY
202 HIMES HALL
BATON ROUGE,LA 70803-0100
Performing Department
Bob R. Jones Idlewild Research Station
Non Technical Summary
The wild pig (Sus scrofa) population continue to negatively impact agronomic crops across the United States and Louisiana to the tune of $1.5 B and $74 M, respectively. Recently, based on a 2013 Louisiana Survey, wild pigs are responsible for production losses of $53 M and increased production costs of $21 M, which accounted for approximately 2% of total farm gate value that year. It has been proposed that due to the reproductive efficiency of feral swine shooting and trapping are not methods that can readily reduce the feral swine population. Outside of the negative impact on crop production, research has indicated that wild pigs pose a significant human health risk. Data has shown that some diseases prevalent in sounders are zoonotic and have been transferred to surface water in national forests.Efforts to control feral swine have been marginal at best and economic impacts to agronomic crops and wildlife continue to be of concern. Current legal methods to control feral swine include trapping, hunting, shooting and snaring, depending on local regulations. However, the population of this invasive species continues to increase. Therefore, alternate methods to be utilized in conjunction with current methods must be developed. Based on the reproductive efficiency of feral swine, 70% of the current population must be removed to hold population numbers at steady state conditions. During the 2015-16 Louisianahunting season 172,300 hogs were removed by hunters, which was greater than the deer harvest (156,000) that same year. Estimates on 2016 feral swine numbers in Louisiana indicate that there were at least 500,000 (possibly higher), theestimate of wild pigs removed by hunters that year equates to only a 34% reduction. Nowhere near the required goal of 70%.Our goal to to produce a wild pig toxicant and delivery system the will effectively remove wild pigs from the landscape while have little if any impact on non-target animals. To that end, wild caught wild pigs will be administered different encapsulated sodium nitrite formulations within the confines of our wild pig facility and number of deaths will be recorded, along with time to death. We will also test several commercially produced delivery systems and our in-house system to determine the impact to non-target animals on the landscape.We anticipate being able to deliver a formulation of encapsulated sodium nitrite that will withstand the rigors of the environment while in a bait station, but release the sodium nitrite within the pigs digestive tract resulting in death. This approach, along with a feeder that does not impact non-target animals, will significantly reduce the wild pig population. The trickle down effect will be less damage to agricultural crops and a decreased probability of zoonotic diseases on the landscape.
Animal Health Component
50%
Research Effort Categories
Basic
0%
Applied
50%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1350899310010%
1350899115050%
1350899118040%
Goals / Objectives
1. Develop and encapsulation protocol for sodium nitrite2. Develop a bait that is preferred by feral swine that will deliver sodium nitrite3. Develop a delivery system for the bait that will negate or reduce the impact on non-target species.
Project Methods
Objective 1: Encapsulation and rapid release as well as taste to encourage feeding must go hand in hand to achieve the acceptable results. Therefore, continued refinement of the SVS-encapsulated SN will be tested on wild caught feral pigs. The current SVS encapsulation protocol will be modified following each trial based on previous results. Wild pigs will be weighed and placed into holding pens containing bait formulations containing SN with a known concentration, based the animals body weight, that would ensure pig's death if consumed. Pigs will be allowed at least 3 hours to consume the bait. Animals will be monitored via remote cameras and consumption and subsequent death rate will be recorded. When bait formulations are not consumed or consumption occurs resulting in no deaths, changes will be made to the encapsulation protocol and subsequent trails will be conducted.Similarly, other encapsulation ingredients and the use of tablet formation will be tested as described above. For tablet formation, tablets will be constructed by combining SN and microcrystalline cellulose followed by compression. The formed tablets will then be film coated to protect the tablet from atmospheric moisture in a film designed to release in the small intestine where uptake and absorption readily occurs. These formed tablets will then be loaded into our bait matrix and pigs will be allowed to consume. Consumption data and death rates will be recorded. As stated above, as trials are completed changes warranted will be addressed and subsequent trails will be conducted.Objective 2: Trials will be conducted to determine bait matrix formulations that pigs will readily consume. Currently, our bait prototype contains dehydrated fish. Trials will be conducted using alternate ingredients and compared with whole-shelled corn, which will serve as the control in all comparisons. On treatment days, individual pigs will be placed in pens and offered differing ingredients/flavors/baits and whole-shelled corn in a paired crossover design. Consumption will be monitored via surveillance cameras. Recorded footage was reviewed and preference will be determined based on the percent of total time feeding on each offered bait. Each the trial will be considered complete when a bait is completely consumed.Objective 3: For this objective I am collaborating with Dr. Randy Price at the Dean Lee Research and Extension Center. A reliable system that is cost effective will be needed to deliver our toxic baits once developed. We believe the only way to deliver baits to target animals is through live feed or semi-live images. Therefore, we will develop a system similar to commercial feral swine traps that send images to a user and the user will control bait deployment from his/her cell phone.Cameras are currently available from commercial entities that utilize SMS texting to control the operation of a drop gate for capturing purposes. Our intention is to identify a camera that we can then program to control the delivery of the bait. More controls are needed on our system as toxic baits will be placed on the landscape and current trapping systems allow for the activation of a single relay to release the gate. The integration of a microcontroller board with programming allowing the user to determine the number of baits to be delivered per tube from a predetermined list is required. Using this approach, the amount of bait spheres placed on the landscape can be limited to what is required for each situation and thus over application of a toxicant can be averted. To achieve this goal,microcontroller boards such as Arduino, Raspberry Pi or similar will be utilized. We anticipate significant programming will have to be accomplished via outsourcing and the stacking of different shields onto the microcontroller board(s) may be necessary to reach the desired level of performance.For this level of performance we anticipate the use of an Arduino UNO or PRO microcontroller board coupled with a Motor shield and a GSM shield. The microcontroller board will contain programming that would control the Motor shield and receive input from the GSM shield. The GSM shield is utilized to connect the Arduino to a GPRS (General Packet Radio Service) wireless network, such as the AT&T system. Once a SIM card is inserted the shield will communicate with the network allowing the transmitting and receiving of text messages. This shield will also communicate with a camera to send motion detected images to the user. The user can then send commands to the GSM shield that will be interpreted by the UNO programming which will compete the desired task either through the camera or the Motor Shield to release bait spheres. This is our initial planned direction, however, because microcontrollers are versatile and different shields can be added or removed from the system, changes in this configuration is expected. Through testing and reconfiguration we are confident that the desired level of performance can be achieved. For this objective I will be collaborating with Dr. R. Price at the Dean Lee Research and Extension Center.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Commodity groups, Soybean and Rice. Stakeholders including cattle producers, crop producers and wildlife enthusiasts. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Results were presented to stakeholders attending forestry forums, crop meetings, consultants meetings, wildlife exposition and field days. What do you plan to do during the next reporting period to accomplish the goals?At present, our bait delivery system will deliver baits to the landscape on demand, however, the delivery of baits must occur over a wider area to allow bait consumption by a larger number of pigs. To achieve this goal, the delivery mechanism will be modified so that bait spheres will be dispersed over an area of approximately 4m2 area. Another method proposed by LDWF which includes the placement of baits within the ground using a bulb auger will be tested. We have successfully used this method and pigs rooted up the baits but did not consume. We believe this was due to the formation of nitrous acid while the bait was exposed to soil and the environment for 48 hours. If successful, this method would allow placement of baits within a rather large area frequented by feral swine without the use of cellular technology and delivery equipment.

Impacts
What was accomplished under these goals? This past year, un-encapsulated sodium nitrite baits were produced and offered to feral swine via our delivery system using whole sounders. The delivery system was set up within the confines of our feral swine pen and loaded with a pre-determined number of baits to provide a 2X-lethal dose to all pigs having access to the delivery system. The delivery system was placed on one side of a wildlife feeder to mimic the user process that would happen on the landscape. As pigs began to use the wildlife feeder as their food source, baits were delivered when pigs were present. Using this approach, we determined two issues that needed to be resolved. First as baits moved through the delivery system pieces of bait left behind due to the mechanical movement of the baits caused the system to become clogged following two or three activations for bait delivery. This in turn negatively impacted the number of baits delivered and the number of pigs that succumb to sodium nitrite poisoning was lower than expected. This issue has been rectified. When baits were delivered in groupsof 8 to 10, one or two animals that were high in the social dominance order consumed most are all of the bait, reducing the number of pigs that had access to baits and resulting in a decreased death rate. Timing of bait delivery to the sounder is now being tested to determine if the delivery of baits over time might increase the number of pigs with access to consume the delivered baits. A second method of bait delivery was attempted. Six to 8-inch holes were drilled in ground using a bulb auger with a diameter of two inches in areas were pigs were known to frequent. Baits were placed in holes and covered. Pigs approached two days later and rooted up baits, these baits were picked up and carried approximately 6 to 10 feet and dropped. This year, plots containing two corn hybrids were planted this year that included 4 treatments and 3 replications. The two hybrids utilized were conventional and round-up ready. Treatments included Aizawai and Kurstaki varieties of Bacillus thuringiensis applied the conventional hybrid, a round-up variety and a non-treated conventional hybrid served as a control. Results showed that 107 days post-planting and 25 days following introduction of feral swine there were no differences in the percent damage across all treatments. However, by 114 days post-planting there was no difference between either the Aizawai or Kurstaki Bacillus thuringiensis treatments, but both were higher than the non-treated conventional hybrid. Likewise, damage to the round-up ready hybrid was higher and any other treatment. By day 121 post-planting all hybrids were almost 100% damaged and there were no differences across treatments. These findings show that pigs may prefer round-up ready corn compared with conventional hybrids, and that treatment of a conventional hybrid with Aizawai and Kurstaki varieties of Bacillus thuringiensis appears to increase preference over that of a non-treated conventional corn hybrid.

Publications


    Progress 10/01/18 to 09/30/19

    Outputs
    Target Audience:Commodity groups, Soybean and Rice. Stakeholders including cattle producers, crop producers and wildlife enthusiasts. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Results were presented to stakeholders attending forestry forums, crop meetings, consultants meetings, wildlife exposition and field days. What do you plan to do during the next reporting period to accomplish the goals?We will continue to test the non-encapsulated baits using our cellular delivery system on whole sounders. Anti-emetics will also be employed, such as taste-free ginger extracts containing gingerols. The incorporationmethemoglobin inhibitors (MR) into the baitwill facilitate control of feral hogs while releasing less nitrite into the environment. Luteolin is a highly-potent, commercially-available, naturally-occurring, tasteless MR inhibitor. Adding small quantities of luteolin to the nitrite-containing bait will reduce a hog's ability to detoxify methemoglobin. Thus the baits can become more potent without adding more toxicant, increasing overall effectiveness.

    Impacts
    What was accomplished under these goals? Corn varieties were planted with differing Bt protection along with a non-modified refuge corn. Pigs appeared to prefer the genetically modified corn compared with the refuge corn. This was an interesting result, in that in previous trials, round-up ready corn was preferred when compared with round-up ready and Bt protection. It appears as though the genetic modification may affect the palatability of field corn for pigs. We foundthat sodium nitrite does not undergo chemical changes in an alkaline environment. This finding allows us to produce and store baits without the need for encapsulation.Individual feeding trails showed that these baits are toxic to pigs and that the pigs prefer the non-encapsulated baits compared with encapsulated baits. The development of the delivery system has progressed. An approach was tested to keep the Louisiana Black bear from consuming sodium nitrite laced baits from a feeder was tested. Results from these tests show that bears cannot gain access to a bucket containing pogie fish. This enhances in our ability to place the delivery system in areas containing bears without fear of intrusion and subsequent death. A more shielded case for the delivery system was developed and toxic baits were dispensed within a enclosure containing a sounder. We recorded the deaths of 30% of pigs.

    Publications


      Progress 11/01/17 to 09/30/18

      Outputs
      Target Audience:Commodity groups, Soybean and Rice. Stakeholders including cattle producers, crop producers and wildlife enthusiasts. Changes/Problems:The use of zinc phosphide was discontinued due to the production of phosgene gas within the digestive tract. This accumulation poses a risk to hunters that dress a wild pig and cut into its digestive tract. What opportunities for training and professional development has the project provided?Training included participation of one graduate student How have the results been disseminated to communities of interest?Results were presented to stakeholders attending forestry forums, crop meetings, consultants meetings, wildlife exposition and field days. Approximately 1,525 stakeholders attended these functions. What do you plan to do during the next reporting period to accomplish the goals?We will continue to develop an ecapsulation protocol which will include our shellac, ethocel and chitosan protocols as well as alkaline water. We will also continue to refine our delivey system.

      Impacts
      What was accomplished under these goals? Concentrations of encapsulated sodium nitrite (SN) resulted in different percent of lethal outcomes compared with non-encapsulated SN. Baits were offered to pigs containing 200 or 400 mg/kg of either encapsulated or non-encapsulated SN, pigs receiving the non-encapsulated SN died regardless of concentration of SN, however, those pigs that received 200 mg/kg of encapsulated SN did not perish, while some of the pigs receiving the encapsulated form at 400 mg/kg survived and others did not. We completed the assessment of zinc phosphide as a toxicant for feral swine. The zinc phosphide was delivered to feral swine via gelatin capsules at 150, 200, 250 and 300 mg/kg and results indicated that we reached a 65% death rate at the 300 mg/kg level. However, time to death was highly variable with an average of 11 hours at the highest concentration, however, some pigs did not succumb until greater than 24 hours following administration. Because of this finding resulting in the elevated risk to hunters that may kill a live pig poisoned with zinc phosphide these experiments were discontinued and zinc phosphide in our opinion is not a good option for feral swine control. Changes in encapsulation of SN laced bait matrices resulted in a 66% of pigs consuming the baits expiring within three hours. However, when SN was encapsulated with Ethocel and offered to pigs in limited trials, no pigs died. In corn hybrid preference trials, we used twelve, 2,700 square foot plots, were planted with either round-up ready or round-up ready, liberty link and Bt corn hybrids. Over the course of the growing season pigs were allowed access to these hybrids and damage was accessed. Based on location within the experimental area pig damage was higher in the round-up ready corn compared with the round-up ready, liberty link and Bt corn. Theratio of pigs damaging the round-up ready only corn ranged from 1.88 to 5.10 times higher compared with the hybrid containing liberty link and Bt. At 82 days post-planting, corn ear worm damage was not different across hybrids, but tended to be different, indicating that secondary metabolites may play a role in pig preference.

      Publications