Progress 09/01/17 to 08/31/20
Outputs
Target Audience:AAVLD, NAHLN and Agriculture stakeholders Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Training in Quality and Annual training Next Gen Sequencing Lab accrediation How have the results been disseminated to communities of interest?Animal Health Conference AAVLD annual meeting What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
(1) Training employees for quality assurance and technical improvements including participation in NAHLN as a Tier 1 lab. Lab has renmained accredited and approved for all NAHLN related testing with expanded capacities 100+ samples in a day. (2) Staff training for foreign and emerging animal diseases: Providing analysis from laboratory for addressing foreign and emerging disease issues. Staff is trained and partcipates in FAD events to address events in PA and surrounding states (3) LIMS and data flow improvement: Barcoding and messaging has been enhanced to message all NAHLN scope diseases. (4) NAHLN sample handling, processing, and testing capacities (including enhancement of biocontainment capabilities): BSL3 space was recommisioned is in full use. (5) Inter-laboratory activities in support of the goals of the NAHLN: Participation in additional methods development and/or method validation and matrix extension work. We intedn to partcipate in interlab comparison and are already a part of Antimicrobial Resistance surveys and NGS work being undertaken by NAHLN.
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Progress 09/01/18 to 08/31/19
Outputs
Target Audience:AAVLD, NAHLN and Agriculture Stakeholders Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Training in Quality at the Annual AAVLD meeting Trainingregarding labaccrediation Next Gen sequencing How have the results been disseminated to communities of interest?Presentations at the State level for helping understand role of Tiers and NAHLN lab structure- County Commssioners meeting Animal Health Diagnostic Conference Bureau of Animal Helath and Diagnostic services field staff What do you plan to do during the next reporting period to accomplish the goals?Quality system: Laboratory is accredited by the American Association of Veterinary Laboratory Diagnosticians. The Quality system continues to undergo continuous improvement. The forms and training records currently that are paper-based are already being digitized. Monitoring of temperature for equipment and equipment calibration and maintenance will be reviewed and addressed for equipment being used for NAHLN and lab testing and requiring calibration service including biosafety cabinets, centrifuges, pipettes, cyclers, liquid handlers and PCR machines. Attendance at the auditors training, AAVLD accreditation, QMS training will be undertaken to strengthen the lab accreditation and quality system. b. Staff training for foreign and emerging animal diseases: Staff will be trained on assays related to foreign and emerging disease analysis. The training will be held both onsite and offsite. Training at the Plum Island and NVSL lab for foreign animal disease and through AAVLD, CDC, ASM, USDA for pathogen testing will be undertaken as an on-going effort to improve response. c. LIMS enhancement: USALIMS used by the lab is being upgraded to version 4.2 Lab would continue to invest in this application. Messaging efforts for all diseases that accept NAHLN messages will continue. Currently lab can message AI and CSF APMV, ASF and FMD tests. NAHLN surveys and exercises have projected the biggest bottleneck can be receiving and data entry. Lab intends to keep working on the goal to enhance barcoding, integration of data transfer into LIMS and reporting enhancements to make data reporting and accessioning easy. d. NAHLN sample handling, processing, and testing capacities (including enhancement of biocontainment capabilities): Lab continues to expand and experiment better methods for increasing throughput including building automation to increase testing capacities. This includes using Eppendorf 5070, Biomek etc and 384 testing well capacity. Laboratory has 30,000+ sq ft and 184 select agent space in our existing building. We have finished recommissioning the 30,000 sq ft BSL3 space. As we improve staffing we will focus on high throughput testing e. Inter-laboratory activities in support of the goals of the NAHLN: Laboratory continues to support interlab comparison activities in support of approval of new methods and laboratory procedures. As new procedures are identified including for TSE and other high consequence pathogen, laboratory intends to participate in inter lab testing, to improve diagnostics and outcomes. We are already making progress on NextGen sequencing and are a participating lab on NAHLN Antimicrobial resistance monitoring project. (5) Inter-laboratory activities in support of the goals of the NAHLN: Participation in additional methods development and/or method validation and matrix extension work.
Impacts
What was accomplished under these goals?
Continue to work on all the project goals (1) Training employees for quality assurance and technical improvements including participation in NAHLN as a Tier 1 lab.: The biggest accomplishment was increasing testsing capacities for NAHLN scope diseases and maintaiing level of proficiency to be able to offer a testing response including for African swine fever virus. Lab has capacity to offer testing on approx 400 PCR tests/day on a sustained basis. (2) Staff training for foreign and emerging animal diseases: Providing analysis from laboratory for addressing foreign and emerging disease issues. Trained pathology staff with local foreighn animal disease training held in Harrisburg. (3) LIMS and data flow improvement: Barcoding and messaging will be enhanced. Messaging, Lab as a tier 1 lab is messaging 5 diseases currently. Barcoding piece has been improved in LIMSand improvements are waiting implementation after hardware is purchased. (4) NAHLN sample handling, processing, and testing capacities (including enhancement of biocontainment capabilities): BSL3 space being renovated will be put to full use for NAHLN work. BSL3 place has been renovated and is undergoing recommissioning. (5) Inter-laboratory activities in support of the goals of the NAHLN: Participation in additional methods development and/or method validation and matrix extension work. Lab is working on high throughput tetsing, genotyping and Next-Gen sequencing implementation.
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Progress 09/01/17 to 08/31/18
Outputs
Target Audience:Animal health Diagnostic Commission meetings at Harrisburg held by Pennsylvania Department of Agriculture Changes/Problems:Hiring of IT staff and Bioinformatics staffhas been slow. What opportunities for training and professional development has the project provided?Training employees for quality assurance and technical improvements including participation in NAHLN as a Tier 1 lab. Staff (Matt Sweger, Deep Tewari ). Staff training for foreign and emerging animal diseases: Providing analysis from laboratory for addressing foreign and emerging disease issues. We are trying to add priortity toenroll staff inupcoming FAD courses. How have the results been disseminated to communities of interest?Animal Health Diagnostic Commission Lab held meetings at the Farm show Horse Expo-2018 What do you plan to do during the next reporting period to accomplish the goals?Continue with all 5 stated goals and strengthening the infrastructure as a Tier 1 lab. Lab has capacity and training and has been responding to FMD test requests and offer a response to SVA testing in pigs. Improve training and participation in regional exercises for developing parternships.
Impacts
What was accomplished under these goals?
LIMS and data flow improvement: Barcoding and messaging will be enhanced. Currently working on this project with the LIMS vendor to integrate barcoding. NAHLN sample handling, processing, and testing capacities (including enhancement of biocontainment capabilities): BSL3 space being renovated will be put to full use for NAHLN work. Space currently under renovation startingJune with 4 month construction time and recommissioning to follow afterwards. Inter-laboratory activities in support of the goals of the NAHLN: Participation in additional methods development and/or method validation and matrix extension work. Lab has joined project on Antimicrobial sueceptibility testing. Lab provided support for AI-USDA WS testing. We are working on multiplex detection and highthroughput testing.
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