Source: PURDUE UNIVERSITY submitted to NRP
UTILIZING REFLECTANCE SPECTROSCOPY TO ASSESS PLANT RESPONSES TO BIOTIC AND ABIOTIC STRESS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1013616
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2017
Project End Date
Sep 30, 2022
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
PURDUE UNIVERSITY
(N/A)
WEST LAFAYETTE,IN 47907
Performing Department
Entomology
Non Technical Summary
Reflectance spectroscopy is emerging as a promising tool with potential applications in agricultural management (Sankaran et al. 2010, Martinelli et al. 2015) because it is a non-destructive, rapid, and a relatively low-cost approach to monitoring vegetation status. In vegetation, reflectance spectroscopy relies on the interaction of light with plant chemical and structural composition and water content (Curan 1989). Reflection of light in the visible (400-700 nm), near-infrared (NIR, 700-1100), and short-wave infrared (SWIR, 1100-2400 nm) can provide a comprehensive assessment of both shifts in visual symptoms (e.g., pigments, leaf color) and the underlying biochemical (e.g., nutrient composition, secondary metabolism) and physiological (e.g., photosynthetic activity, water relations) responses to disease or stress (Couture et al. 2013, 2015, Arens et al. 2017; Cotrozzi et al. 2017). Spectroscopy can also aid in the retrieval of plant traits, and has been utilized to predict a wide range of plant biochemical constituents and physiological processes based on the optical properties of foliage, including concentrations of nutrients and secondary metabolites and leaf structural and morphological composition (e.g., Petisco et al. 2006, Asner and Martin 2008, Kleinebecker et al. 2009, Asner et al. 2011, Couture et al. 2013, 2016, Serbin et al. 2015) and plant physiological status or specific physiological processes (Gamon et al.1997, Serbin et al. 2012, Cotrozzi et al. 2017).To date, the applications of spectroscopy in agriculture have largely focused on disease detection (e.g., Naidu et al. 2009, Mahlein et al. 2012, Arens et al. 2017) through classification strategies (e.g., Rumpf et al. 2010), development of narrow- or broad-band disease indices (e.g., Mahlein et al. 2013), or relating vegetation indices sensitive to plant stress with plant disease status (e.g., Huang et al. 2007). In the same manner as disease classification, hyperspectral data is also utilized for classification of plant varieties and genotypes (e.g., Li et al. 2007, Li & He 2008). Less is know about the ability of hyperspectral data to classify differences in plant stress responses between stressors that produces similar physiological effects, an emergent outcome of the proposed research. The use of hyperspectral data to detect specific biochemical and physiological responses to disease in crop systems is less explored and represents a promising and novel approach for not only the classification of disease status via spectral profiles, but also non-destructively quantifying responses of plants to stress.
Animal Health Component
20%
Research Effort Categories
Basic
70%
Applied
20%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2032499113013%
2032499116013%
2032410113012%
2032410116012%
2112499113013%
2112499116013%
2112410113012%
2112410116012%
Knowledge Area
211 - Insects, Mites, and Other Arthropods Affecting Plants; 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants;

Subject Of Investigation
2410 - Cross-commodity research--multiple crops; 2499 - Plant research, general;

Field Of Science
1130 - Entomology and acarology; 1160 - Pathology;
Goals / Objectives
The goal of this research is to evaluate the ability of hyperspectral data as a potential tool for plant management of agricultural and natural areas. Specifically, I will determine the ability of spectroscopy to:1) Detect asymptomatic responses by plants to biotic and abiotic stress, i.e., before the occurrence of visual symptoms.2) Identify specific biochemical and physiological responses by plants to biotic and abiotic stress that allow spectral information to detect stress.3) Determine the ability of hyperspectral data to accurately classify multiple different stressors, alone and in combination.4) Determine that ability of hyperspectral data to achieve points 1, 2, and 3 accurately across multiple plant developmental stages.4) Evaluate the ability of field-based approaches for points 1, 2, 3, and 4 across multiple measurement scales.
Project Methods
I plan to assess the ability of spectral data to 1) accurately phenotype plant responses to biotic and abiotic stress, 2) determine how well the outcomes from 1 translate across development stages, and 3) different measurement scales. To address 1, I will characterize the foliar spectral profiles of individuals from multiple plant types, selecting multiple varieties from each species that display a range of stress responses, and will expose subsets of the plants to combinations of stressors, allowing for the assessment of spectral data to classify stressors that potentially have the same physiological effect on the plant. To address 2, I will monitor 1 over multiple plant developmental stages. To determine if the outcomes from 1 and 2 scale across measurement scales, with spectral measurements made on both individual leaves, using single plants, and at the canopy-scale, using multiple plants. This comparison across spatial scales will allow us to determine consistent variations in spectral profiles that can be used in the spectral phenotyping of plant stress detection across multiple measurement scales, allowing for the potential application of this approach at larger, field-based spatial scales.Measurements will be both field- and greenhouse-based, at different facilities at Purdue and will include both planned measurements and opportunistic measurements when available. This research will also utilize the resources at the newly constructed field-phenotyping facility (Indiana Corn and Soybean Innovation Center) at the Agronomy Center for Research and Education in West Lafayette, IN.Foliar and canopy spectral measurements will begin one week after treatment application and will continue weekly until plant senescence using a full-range, portable spectroradiometer (1024i, Spectra Vista Corporation, Poughkeepsie, NY, USA) possessed by PI Couture. In field and chamber conditions, leaf-level spectral measurements will be made using fiberoptic cable and a leaf-clip attachment with a calibrated, stable light source and, in the field, canopy spectral measurements will be made using a 14 degree foreoptic with field of view and height above canopy standardized using a tripod mount. Differences in spectral profiles across treatments and spatial and temporal scales will be analyzed using a variety of statistical analyses (e.g., permutational multivariate analysis of variance) and classification approaches (partial least-squares discriminate analysis). Additional complimentary measurements will be made with spectral measurements to gain a better understanding of the factors underlying shifts in spectral profiles, including samples of foliar tissue for chemical analyses (e.g., nitrogen, lignin, phenolic compounds) and measurements of physiological responses (e.g., water potential, stomatal conductance, photosynthesis). These measurements will also allow for expanding the species and environment range for modeling traits using spectral data. Novel trait algorithms will be developed using partial-least-squares regression and the strength of the relationship among spectral variability and treatments or traits will be quantified, for example using the correlation of the normalized differential spectral index (NDSI), calculated as NDSI=[(wavelength i + wavelength j) / (wavelength i - wavelength j)] with the plant status or trait of interest.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Audiences reached have included scientific audiences through presentations at conferences and peer-reviewed publications and stake holder audiences through extension and outreach events and field days. Changes/Problems:Coivd has slowed research progress on certain experiments and has made conference presentations challenging. Progress is still occuring but much slower than pre-Covid activity levels. What opportunities for training and professional development has the project provided?To date, this project has provided training for twopostdoctoral scholars, fsixgraduate students in my lab, and twelveundergraduate research assistants, and twotechnicians. One student has graduated with a masters of science degree. Both the posdoctoral scholars and the graduate students have participated in multiple scientific and professional development conferences and outreach/extension events. One graduate student has submitted a fellowship grant to NSF in 2018 How have the results been disseminated to communities of interest?Results have been disseminated primarly through scientific and extension conferences and peer-reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?What do you plan to do during the next reporting period to accomplish the goals? Data collected form the projects initiated will be analyzed and presented at scientific conferences and published in peerreviewed journals. New experiments will be initiated based on the results from the current projects or as need/opportunity arise.

Impacts
What was accomplished under these goals? Existing data has been published or presented regarding previsual detection of stress using hyperspectral data. My program has completed several experiments related with the major goals of this project. Specifically we have initiated projects examining the ability of hyperspectral data to characterize the impact of 1) fertilization on wheat nutrient status in the field at different developmental stages and measurement scales; 2) insect infestation on cropfunctional trait responses; 3) drought stress on maize functional responses ; 4) nutrient availability and pathogen and water stress on functional trait responses of industrial hemp; 4) disease status and nutrient and water stress on black walnut functional responses; 5) assess the ability of hyperspectral data to classify different stressors, alone and in combination; and 6) use remote sensing tools to asses plant disease and variation in plant functional traits.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Mason CJ, Walsh B, Keller J, Couture JJ, Calvin D, Urban JM (2020) Fidelity and timing of attack patterns of spotted lanternfly (Lycorma delicatula) in ornamental trees in the suburban landscape. Environmental Entomology doi: 10.1093/ee/nvaa109
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Cotrozzi L, Peron R, Tuinstra MR, Mickelbart MV, Couture JJc (2020) Spectroscopic determination of physiological and anatomical leaf traits related with water status in maize. Plant Physiology 184:1363-1377.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Williams LJ, Cavender-Bares J, Townsend PA, Couture JJ, Wang Z, Stefanski A, Messier C, Reich PB (2020) Remote spectral detection of biodiversity effects on forest productivity. Nature Ecology and Evolution DOI 0.1038/s41559-020-01329-4
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2020 Citation: De La Mater D, Couture JJ, Puzey JR, Dalglesih HJ (2020) Effects of range-wide, intraspecific variation in functional traits on plant-herbivore interactions. American Journal of Botany in press
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ricono A, Gustafson NW, Eichenberger E, Stahl K, Call H, Couture JJ, Puzey JR, Dalgleish HJ (2020) Fine-scale spatial structuring of genotypes and phenotypes in natural populations of Asclepias syriaca. Perspectives in Plant Ecology, Evolution and Systematics 125546.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Wang Z, Chuls A, Geygan R, Ye Z, Zheng T, Singh A, Couture JJ, Cavender-Bares J, Kruger EL, Townsend PA (2020) Foliar functional traits from imaging spectroscopy across biomes in eastern North America. New Phytologist 228:495-511.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Gold KM, Townsend PA, Chuls A, Hermann Ip, Couture JJ, Larson ER, Gevens AJ (2020) Hyperspectral reflectance sensors for pre-symptomatic detection and differentiation of Phytophthora infestans and Alternaria solani in potato. Remote Sensing 12:286.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Li Z, Yang J, Shang B, Xu Y, Couture JJ, Yuan X, Kobayashi K, Feng Z (2020) Effects of elevated tropospheric O3, water deficiency and nitrogen addition on phytochemical composition in Poplar saplings. Science of the Total Environment 707:35935.
  • Type: Journal Articles Status: Submitted Year Published: 2020 Citation: Gustafson NW, Couture JJ, Dalgleish HJ (in review) Both plant traits and nectar chemistry affect the community of insect visitors and pollination success in common milkweed (Asclepias syriaca). Journal of Ecology
  • Type: Journal Articles Status: Submitted Year Published: 2019 Citation: Bolt MA, Beckerman JL, Couture JJ (in review) Agronomic management of industrial hemp alters foliar traits and herbivore performance. Arthropod-Plant Interactions


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Audiences reached have included scientific audiences through presentations at conferences and peer-reviewed publications and stake holder audiences through extension and outreach events and field days. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?To date, this project has provided training for one postdoctoral scholar, fivegraduate students in my lab, and tenundergraduate research assistants. One student has graduated with a masters of science degree. Both the posdoctoral scholar and the graduate students have participated in multiple scientific and professional development conferences and outreach/extension events. One graduate student has submitted a fellowship grant to NSF in 2018. How have the results been disseminated to communities of interest?Results have been disseminated primarly through scientific and extension conferences and peer-reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?Data collected form the projects initiated will be analyzed and presented at scientific conferences and published in peer-reviewed journals. New experiments will be initiated based on the results from the current projects or as need/opportunity arise.

Impacts
What was accomplished under these goals? Existing data has been published or presented regarding previsual detection of stress using hyperspectral data. My program has completed several experiments related with the major goals of this project. Specifically we have initiated projects examining the ability of hyperspectral data to characterize the impact of 1) fertilization on wheat nutrient status in the field at different developmental stages and measurement scales; 2) Hessian fly infestation on wheat functional trait responses; 3) drought and western corn rootworm on maize functional responses and the ability of hyperspectral data to classify each stress independently and interactively; 4) nutrient availability and pathogen and water stress on functional trait responses of industrial hemp;4) disease status and nutrient and water stress on black walnut functional responses; 5) assess the ability of hyperspectral data to classify different stressors, alone and in combination; and 6) use remote sensing tools to asses plant disease and variation in plant functional traits.

Publications

  • Type: Journal Articles Status: Accepted Year Published: 2018 Citation: Rotter MC, Couture JJ, Rothwell EM, Garcia Ju, Holeski LM (2018) Plant resistance traits across the herbivore diet breadth spectrum: A test in the model plant Mimulus guttatus. Evolutionary Ecology Research 19:423-440
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Wang Z, Townsend PA, Schweiger AK, Couture JJ, Singh A, Hobbie SE, Cavender-Bares, J (2019) Foliar functional traits across three years in a prairie grassland. Remote Sensing of Environment 221:405-416
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Cotrozzi L, Couture JJ (2019) Hyperspectral assessment of plant responses to multi-stress environments: prospects for managing protected agrosystems. Plants, People, Planet in press
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Gongora-Canul C, Salagado JD, Singh D, Cruz AP, Cotrozzi L, Couture JJ, Cruppe G, Rivadeneira MG, Valent B, Todd B, Poland J, Cruz CD (2019) Temporal dynamics of wheat blast and agreement between remotely sensed data measurements and visual estimations of wheat spike blast (WsB) under field conditions. Phytopathology accepted
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: School of Integrative Biology, University of Illinois Urbana-Champaign. Using hyperspectral information for understanding plant stress.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: Entomological Society of America North Central Branch Meeting, Cincinnati, OH. Utilizing spectroscopy for food security. Peron R, Cotrozzi R, Tunistra M, Micklebart M, Mohammadi M, Russel B, Stuart J, Campos-Medina V, Couture J.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: Entomological Society of America North Central Branch Meeting, Cincinnati, OH. Influence of agronomic management practices on hemp insect interactions. Bolt M, Beckerman J, Couture J
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Entomological Society of America Joint Annual Meeting, Vancouver, B.C., Canada. Integrating hyperspectral data into hemp management. Couture J, Bolt M, Beckerman J
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Entomological Society of America Joint Annual Meeting, Vancouver, B.C., Canada. Impact of agronomic management decisions on industrial hemp foliar quality and insect performance. Bolt M, Beckerman J, Couture J
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Struckman S, Couture JJ, LaMar MD, Dalgleish HJ (2019) The demographic effects of functional traits: An integral projection model approach reveals population-level consequences of reproduction-defense tradeoffs. Ecology Letters 22:1396-1406
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Campos Medina VAg, Cotrozzi Lp, Stewart JJ, Couture JJ (2019) Hyperspectral characterization of Hessian Fly infestation on wheat functional trait responses. PlosONE https://doi.org/10.1371/journal.pone.0219431


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Audiences reached have included scientific audiences through presentations at conferences and peer-reviewed publications andstake holder audiences through extension and outreach events and field days. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has provided training for one postdoctoral scholar, three graduate students in my lab, and eight undergraduate research assistants. Both the posdoctoral scholar and the graduate students have participated in multiple scientific and professional development conferences and outreach/extension events. One graduate student has submitted a fellowship grant to NSF. How have the results been disseminated to communities of interest?Results have been disseminated primarly through scientific and extension conferences and peer-reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?Data collected form the projects initiated will be analyzed and presented at scientific conferences and published in peer-reviewed journals. New experiments will be initiated based on the results from the current projects or as need/opportunity arise.

Impacts
What was accomplished under these goals? Existing data has been published or presented regarding previsual detection of stress using hyperspectral data. My program has completed several experiments related with the major goals of this project. Specifically we have initiated projects examining the ability of hyperspectral data to characterize the impact of1) fertilization on wheat nutrient status in the field at different developmental stages and measurement scales; 2)Hessian fly infestation on wheat functional trait responses; 3) drought and western corn rootworm on maize functional responses and the ability of hyperspectral data to classify each stress independently and interactively; 4) nutrient availability andpathogenand water stress on functional trait responses of industrial hemp; and 4) disease status and nutrient and water stressonblack walnut functional responses.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Couture JJ, Singh A, Charkowski AO, Groves RL, Gray SM, Bethke PC, Townsend PA (2018) Integrating spectroscopy with potato disease management. Plant Disease 102: 2233-2240.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Blood BL, Paschen MA, Klingeman WE, Had~iabdi? ?, Couture JJ, Ginzel MD (2018) Behavioral responses of Pityophthorus juglandis (Coleoptera: Curculionidae: Scolytinae) to volatiles of black walnut and Geosmithia morbida, the causal agent of Thousand Cankers Disease. Environmental Entomology 47:412-421.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Cotrozzi L, Townsend PA, Pellegrini E, Nalia C, Couture JJ (2018) Reflectance spectroscopy: a novel approach to better understand and monitor the impact of air pollution on Mediterranean plants. Environmental Science and Pollution Research 25:8249-8267
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Department of Biological Sciences, Western Michigan University, Kalamazoo, MI. Integrating spectroscopy with plant ecology.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI. Utilizing spectroscopy to identify plant stress.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Ecological Society of America, New Orleans, LA. Hyperspectral detection of plant chemical defenses and responses to herbivory.