Source: STONY CREEK COLORS, INC. submitted to NRP
BIO-BASED INDIGO AGRICULTURAL SUPPLY CHAIN DEVELOPMENT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1013561
Grant No.
2017-33610-27023
Cumulative Award Amt.
$599,828.00
Proposal No.
2017-03318
Multistate No.
(N/A)
Project Start Date
Sep 1, 2017
Project End Date
Aug 31, 2019
Grant Year
2017
Program Code
[8.12]- Small and Mid-Size Farms
Recipient Organization
STONY CREEK COLORS, INC.
3456 KNIGHT DR
WHITES CREEK,TN 371899188
Performing Department
(N/A)
Non Technical Summary
Stony Creek Colors sells clean and safe natural dyes to the textile and fashion industries, allowing its customers to offer environmentally conscious premium products. Through its flagship natural indigo product, the company has developed and proven a complete agricultural supply chain to replace synthetic dyes with plant-based drop-in solutions. Innovations across the complete crop and processing value chain allow Stony Creek to sustainably produce plant-based dyes with full transparency and authenticity, meeting the technical performance and volume requirements of textile mills and fashion brands worldwide without compromising product integrity. A guaranteed take-off agreement with a leading global denim mill partner ensures the company's bio-based dyes are in demand, and the naturally dyed denim resulting from this effort is currently being used in premier brands. Stony Creek Colors has demonstrated the commercial viability of the natural indigo supply chain having significantly scaled its operations in 2016, and it is now identifying and executing upon efficiencies made evident in the plant genetics, agricultural operations, and chemical processing activities. Through this NIFA/USDA-funded research effort, Stony Creek Colors is working to optimize the agricultural and industrial natural indigo supply chain by identifying, establishing, and refining scalable and efficient methods of indigo crop production and factory extraction which will allow bio-based indigo the opportunity to compete more closely with its synthetically manufactured counterpart.Natural indigo was once a driver of the world economy, with plantations existing across the globe which grew, cultivated, harvested and processed indigo crops into blue indigo dye. This was usually done through a multi-step but industrially simple and environmentally low-impact process. Following the discovery of a synthetic pathway to indigo dye at the turn of the 20th century, this agricultural crop and its bio-based dye product were quickly supplanted by a commodity industrial chemical synthesized from coal tar and petroleum derivatives. The textile dyeing and finishing industry is now responsible for 17% of industrial water pollution worldwide, and after the turn of the 21st century, consumers are becoming increasingly conscious of the impact of their personal choices allowing socially and environmentally sustainable products to take market share. Major fashion brands like Levi's & Nike are now making purchasing decisions based on the full environmental and life cycle impact of their products, with a commitment to reduce hazardous chemical use to zero by 2020. Natural indigo is an obvious entry into this new market opportunity, representing a known story that once defined the denim jeans market. The incorporation of natural indigo into an existing brand or line of clothing represents a low-cost differentiator with significant customer resonance allowing for higher margins for mills and brands. However, as an agricultural crop and specialty bio-based product, consistent, reliable, and sizeable sources for natural indigo dye no longer exist in the global marketplace. Natural indigo dye and its parent agricultural crop have missed out on over 100 years of development in chemistry, chemical engineering, and agricultural science. Stony Creek Colors has applied advancement, innovations, and know-how in modern chemistry to develop and validate a proprietary, safe, high-yielding, and chemically benign extraction process for converting indigo crop biomass into high-value, high-consistency natural indigo dye. The company has further developed a network of small- and medium-sized farmers in the Northern Middle Tennessee area to successfully grow indigo as a transplant-crop using tobacco growing assets. Stony Creek Colors scaled this structure in 2016 to 26 acres of indigo production crop and 10,000 sqft of factory production.Phase I of this NIFA/USDA-funded SBIR project served to address challenges to scaling commercial indigo production including needs for 1) consistent and cost effective field establishment of natural indigo crops including replacement of transplanting with direct seeding, 2) scalable mechanized harvesting of indigo crops without damage to the leaves, 3) a modern and comprehensive agronomic management system, 4) improved scalable factory unit operations, and 5) blending strategies for indigo crops for product consistency across batches. Phase I results were generally successful, resulting in implementation of key technologies for the 2017 scale up to 180 acres of crops and a factory throughput of roughly 70,000 pounds of indigo plant biomass per day for at least 120 days of the season. Phase I advancements in crop establishment, harvesting, and transportation have allowed for sustainably managed Japanese indigo contract farm sizes to increase from an average 2.9 acres in 2016 to 20 acres in 2017, with new tropical indigo farms averaging over 6 acres per farmer and returning profitable crops to what were once high-value tobacco fields. Phase II efforts will serve to refine the technology outcomes from Phase I, and to refine the strategies and methods for their implementation.
Animal Health Component
40%
Research Effort Categories
Basic
10%
Applied
40%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
51122992000100%
Goals / Objectives
Research and development will focus paid to improved and consistent crop establishment, scalable mechanical harvesting and transport of biomass, logistic and agronomic management systems, finalized and efficient factory unit operations, and high-performing tropical indigo incorporation. Each of these represents an opportunity for significantly improving the efficiency and efficacy of the natural indigo supply chain and making it industrially competitive as a premium alternative to synthetic indigo dye.The Phase II goals are specific innovations that correct for inconsistencies in the raw material base by developing improved agronomic practices and methods to improve batch consistency. The outcome of this research will provide a foundation for Stony Creek Colors to not only meet future production targets but also to significantly improve its understanding of how indigo crop production systems can be improved and scaled. The work conducted under this proposal will serve as a basis for developing optimized systems for industrial production of indigo that can reduce the overall commercial crop production costs, risks, and variability for small farmersTechnical Objective 1: Crop EstablishmentStony Creek Colors has demonstrated the ability to leverage know-how and expertise existing within the network of tobacco farmers in the Northern Middle Tennessee are to successfully deploy and manage indigo as a specialty crop. However, the transplanting aspect of this method limits the potential scale of the crop to growers with access to greenhouses and transplant setters. In order to expand the potential reach of this crop, methods are being pursued for planting seed using methods more typical to non-tobacco farmers. In this Technical Objective, Stony Creek Colors will compare existing crop establishment techniques with more scalable direct seeding methods or higher density transplant methods, to include a range of seeding methods, dates, field conditions, and seed coatings and weed control treatments. The objective is to determine what circumstances and methods can result in efficient high-density stand establishment.Technical Objective 2: Advanced Mechanical Harvest and TransportTraditionally, indigo crops have been hand-harvested using low-cost labor. This is not an option for a U.S.-grown specialty crops, and it does not philosophically align with Stony Creek's goals. To mechanize the indigo harvest for industrial scale, methods were evaluated to carefully remove the leaves from the plants without causing significant damage, leaving the living stems intact. In this Technical Objective, the company will evaluate the mechanical harvest method identified through Phase I research and development with regard to scale up to commercially available self-powered combine-type harvesters. It is critical that a replicable and scalable mechanical harvest method is identified and fully investigated as a unit component of the operational model.Technical Objective 3: Logistics and Agronomic Management SystemsJapanese and tropical indigo are new specialty crops which have missed out on 100 years of agricultural advancements. To rapidly make this crop industrially viable and scalable to 20,000 acres of small- and medium-sized farms, crop data must be continually collected and assessed in near-realtime to inform decisions and enable predictive analysis in the farmers' fields and at the processing facility. Applying modern data technology to the indigo supply chain will generate realtime targets and performance indicators, refine equations to drive predictive analytics for plant and factory yields, and enable efficient logistics operations as fleets of harvesters and trailers bring crops from the fields to the factory with optimization for throughput and output.Technical Objective 4: Factory Unit OperationsBecause no legacy infrastructure exists for the manufacture of indigo dye from natural indigo crops, Stony Creek Colors has developed a proprietary safe and sustainable dye extraction process and has scaled it to industrial factory unit operations. During the scale-up of this process from laboratory to factory, new unit operations are identified as operational bottlenecks and are solved. After Phase I research and development and successful 2016 factory season, the bottleneck was moved from efficient settling tank separation to final dewatering of the product slurry. The objective of this research area is to evaluate and validate various industrial operations and equipment for providing the final product in a usable state, and optimizing the slurry entering that final solid-liquid separation.Technical Objective 5: Tropical Indigo Processing and Post-Production BlendingDuring the course of Phase I research, tropical indigo varieties were evaluated as potential season-extending, single-harvest crops, though the research conducted indicates the potential for this crop to play a more significant role requiring additional research. Not only were product purities expectedly higher than indigo, but leaf quality, plant yield, and batch yield were also higher. The data collected during the 2016 season is heavily weighted towards end-of-season activities and therefore does not provide an adequate baseline for predictive analysis and does not inform the 2017 crop makeup. This Technical Objective seeks to collect the necessary dataset for tropical indigo production to make it a potential alternative high yield crop.
Project Methods
Stony Creek Colors currently operates a bio-based dye production factory, and chemistry laboratory, and a small research farm, and contracts indigo crop production to small and medium farmers in Northern Middle Tennessee. The focus of the technical objectives presented in this project is to improve effiency and capability at all points along the process, and as such, much of the research will be carried out along side existing processes in the current facilities and farm. New process equipment will be rented and evaluated with representative raw materials in a factory setting. Seed and plant trials will be perfromed along side Stony Creek's standard plant breeding and optimization trials in production greenhouses and research plots. Laboratory analyses will be conducted by Stony Creek's research chemists as part of their daily routine.

Progress 09/01/17 to 08/31/19

Outputs
Target Audience:Much of the work performed by Stony Creek Colors went to improve its own processes, but the work relating to on-farm research, indigo agronomics, and methods for increasing the yield and quality of indigo crops all directly effected the operations of the company's contract farmers. At a broader scope, these famrers along with the farmers potentially involved in Stony Creek's future production have also benefited from the company's efforts to make natural indigo dye a more viable and widely accepted specialty bioproduct, creating a market for this high value alternative or rotational crop. Changes/Problems:No substantial changes or problems encountered. What opportunities for training and professional development has the project provided?While the project was not designed with a goal of providing training and professional development, the Phase I and Phase II projects have provided opportunities for greenhouse and laboratory technicians to learn skills, analysis methods, and knowledge related to general chemistry and biology as well as areas of functional expertise. How have the results been disseminated to communities of interest?Results of the Phase I and II projects have produced changes to Stony Creek Colors' agricultural and factory operations. With respect to agricultural improvements, these year-on-year improvements have been disseminated to the contract farmer network through published Grower Guidelines and field days at farmers' production fields and Stony Creek's research field. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Task 2: Evaluate direct seeded plant performance against traditional transplant crop Methods for direct seeding were tested over multiple seasons. Japanese indigo trials were unsuccessful due to a shorter growing period and lack of selective herbicide. Tropical tests were also unsuccessful due to extremely low in-field germination and a single harvest opportunity per plant. Task 3: Evaluate the impact of double seeding at traditional transplanter spacings The results of Task 3 research are shown in Task 1. Task 4: Contract with a local growing partner to evaluate direct seeding into cover Japanese indigo was drilled into terminated cover crop and compared with indigo drilled at the same date in an adjacent tilled plot. Both plots experienced similar levels of weed competition, requiring mechanical cultivation due to lack of useful herbicides. Plants grew indistinguishably between the two plots, so no conclusion of benefit was drawn. Task 5: Contract with a local growing partner to evaluate fall and winter direct seeding Post-frost seeding trials were conducted at research and production fields for Japanese and tropical varieties. In both cases, fields were tilled under and then seeded with no cover crop. Weed competition in both varieties was uncontrollable, and pre-emergent chemicals were not usable. This method was concluded ineffective for growing indigo crops. Task 6: Evaluate pretreatments and seed pelleting for direct seeding and greenhouse operations Low and inconsistent seed germination rates were addressed through chemical scarification and plant hormone mimicry for tropical and Japanese varieties. This in combination with improved seed presorting yielded substantially improved results. Task 7: Conduct a revised herbicide screen for Japanese indigo Herbicide screens were conducted for tropical and Japanese varieties using commonly used or well-suited herbicides in pre- and post-applications, tracking plant height and health. Zidua showed no negative impact in Japanese trials, while Staple LX showed promising results in tropical plants. Task 8: Evaluate clonal plug propagation of Japanese and tropical varieties Results from a limited trial of clonal transplants showed virtually no difference in the size, health, survivability, and indigo yield between cloned and seed-started plants. However the commercial viability of industrial scale transplant production appears not economical. Research Area 2 - Advanced Mechanical Harvest and Transport Task 1: Evaluate rate of harvest for self-propelled leaf stripping harvester Using a leaf-stripping combine designed from the Phase I tests, tropical and Japanese crops were harvested at densities up to 22,000 plants per acre and a rate of 2-4 acres per hour. Optimal plant conditions for harvesting proved to be more tied to harvester geometry and height than a target plant biomass. Task 3: Evaluate impact of mechanical stripping on the regrowth rates SCC harvests leaf biomass from plants 3 times per season, assuming stripped plants regrow faster than flush-cut plants. This bore out in the research field, demonstrating that stripped plants closed canopy more quickly and resulted in more biomass harvested per plant per season. Task 4: Evaluate various trailer designs with regards to in-field loading of biomass, the ability to utilize the trailer as a soaking vessel, and the ability to safely unload spent biomass SCC designed and validated a trailer that could receive biomass from the harvester's integrated dump cart, transport the biomass to the factory, serve as a watertight primary extraction vessel, and then transport the spent biomass to the compost site where it is tilted and dumped through an end gate. Research Area 3 - Logistics and Agronomic Management Systems Task 1: Establish and make available through software key performance indicators for agricultural, logistics, and factory operations Stony Creek designed a comprehensive data collection system for ag, logistics, and factory operations which is supported by database systems for chemistry lab analysis activities. This tool is designed to aggregate, process, and provide actionable data to inform the calendar of harvests and to provide predictive indications of factory yields. Task 2: Build a tool to utilize crop performance data and harvest capabilities to inform optimal harvest dates and expected factory yields Included with Task 1. Task 3: Build a tool to manage harvest schedules, transport schedules, and factory throughput Included with Task 1. Research Area 4 - Factory Unit Operations Task 1: Evaluate equipment for final solid-liquid separation including centrifuges, pressure filtration, and tray dryers After evaluation, Stony Creek purchased and implemented large scale filter presses for final dewatering operations. Task 2: Evaluate magnesium chloride in industrial operation Mag chloride was evaluated and performed better than alum with regard to cut and decant clarity, though the floc was loosely bonded resulting in difficulties with downstream filtration at pressure. Task 3: Submit final product to post-processing converter to test use in saleable product Tray-dried batches settled with alum and magnesium chloride were provided to the converter for evaluation, and both were usable in their 95% dry, as-delivered form. Task 4: Evaluate final conversion processing in-house including grinding, blending, chemical dispersant and pH modifier addition SCC evaluated and validated the ability to economically process its crude product into a final paste form. Research Area 5 - Tropical Indigo Processing and Post-Production Operations Task 1: Perform full season tropical indigo growth and regrowth analyses Presented under Research Area 2, Task 3 Task 2: Evaluate direct seeding methods and treatments validated for Japanese indigo in order to determine tropical indigo viability as a non-transplant crop in the Middle Tennessee climate. SCC attempted direct seeding of tropical indigo with no success. In 2019, the company was able to create a successful stand, but it was rapidly overtaken and outcompeted by weeds Task 3: Perform third-party validation of Japanese and tropical indigo sample purity to confirm efficacy of Stony Creek Colors' purity analysis protocol. SCC developed and refined its UV-Vis -based non-reductive analysis method. Samples analyzed with this proprietary method were then sent to AB Tech for HPLC analysis. Results were validated within less than 1%. Task 4: Incorporate the tropical indigo dataset into the harvest timing and logistics tools. Over 1,000 growth and yield data points were assessed and entered into the predictive modeling tool.

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