Source: SOUTH DAKOTA STATE UNIVERSITY submitted to NRP
COMPETITIVE ABILITY OF TWO EMERGING INVASIVE PLANTS IN MIXED-GRASS PRAIRIE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1013450
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2017
Project End Date
Sep 30, 2022
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
SOUTH DAKOTA STATE UNIVERSITY
PO BOX 2275A
BROOKINGS,SD 57007
Performing Department
Natural Resource Management
Non Technical Summary
Non-native plant invasions create significant ecological and economic impacts. Biological invasions occur when a non-native species is introduced to a new environment, grows into a sustained population, and this population creates problems. Ecologically, invasive species alter community structure, alter ecosystem processes such as hydrology, nutrient cycling, and fire regimes. Specifically, invasive plants in South Dakota have been observed to decrease wildlife habitat quality, substantially lower native species diversity, and reduce forage abundance for livestock. Beyond ecological impacts, species invasions generate economic impacts estimated to be greater than $100 billion annually in the US as a result of costs to control the invasion, reduced recreational and aesthetic opportunities, and lost livestock and crop production. Identification of traits that contribute to invasion enable more informed and precise management of invasive species. Competitive ability is often a trait that enables newly introduced species to become dominant in a new environment. Competitive ability encompasses both direct and indirect effects of the invader on neighbors. The hypothesis that invasive species have higher competitive ability than co-occurring native species is long standing with documented support in many ecosystems. An invader with a large competitive effect (i.e., ability to suppress neighbors) and a large competitive response (i.e., avoidance of suppression due to neighbors) will have a high potential to become invasive. It is worthy to note that allelopathy, or the direct chemical inhibition of one by plant by another, is not a mechanism of competition. A species with higher competitive ability may be more likely to invade resource rich sites and sites with higher biodiversity than species without this ability. South Dakota grasslands tend to be fairly resource rich and biodiverse and therefore may be especially vulnerable to invasion of species with high competitive ability. Crown vetch (Securigera varia synonym Coronilla varia) is a non-native perennial legume that has been planted for erosion control or as forage. Crown vetch can become invasive, spreading from where it was planted both by seed and rhizome. The dense monospecific stands created by crown vetch decrease native plant richness, performance, and biomass; modify soil nutrient cycling; and alter available fuel loads. Crown vetch has very high competitive ability, even outcompeting Canada thistle (Cirsium arvense) in some settings. Russian olive (Elaeagnus angustifolia) was introduced in the early 1900's as a horticulture tree from central Asia to the United States. Russian olive now occurs in rangelands and riparian areas in the Great Plains, the Intermountain West, and western Canada. Federal programs between 1930 and 2002 encouraged land managers to plant Russian olive in US grasslands to provide windbreak and stabilize soils. However, ecological research suggests that Russian olive creates negative ecological impacts. Russian olive has been observed to alter vegetation dynamics by limiting recruitment of shade-intolerant native tree and shrub species. In riparian areas of southeastern Alberta and north-central Montana, Russian olive has almost completely replaced the native Plains cottonwood (Populus deltoides). Russian olive has also been observed to alter the soil microbial community and alter nutrient cycles in both terrestrial and aquatic systems.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
12107801070100%
Knowledge Area
121 - Management of Range Resources;

Subject Of Investigation
0780 - Grasslands, other;

Field Of Science
1070 - Ecology;
Goals / Objectives
The major goal of this project is to elucidate the competitive ability of two non-native plants in South Dakota that have previously received little research attention. The supporting objectives are:Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants.Determine if soil solarization effectively reduces the seedbank of non-native crown vetch (Securigera varia).Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species.
Project Methods
Objective 1: Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants. A standard 'cage-match' design will be used to determine the competitive ability of crown vetch compared with co-occurring natives (options include Echinacea angustifolia, Dalea purpurea, Monarda fistulosa, Cleome serrulata, Ratibida columnifera, Liatris aspera, Gaillardia aristata, and Aster ericoides). In this experiment, growing with a neighbor will be considered the 'treatment' and plants growing alone will be considered the 'control'. Within a greenhouse located at South Dakota State University's main campus in Brookings, SD, crown vetch and native seeds will be planted in containers (Deepot 40, Steuwe and Sons, Corvallis OR) containing field-collected topsoil. The goal of the cage-match design is to have one crown vetch individual and one native individual in each pot. In case germination of the seeds is not 100%, several seeds of each species will be planted per pot to ensure that at least one plant of each species establishes. Within each treatment pot, multiple seeds of crown vetch will be seeded in the center of the pot and multiple seeds of one native species will be planted 2.5 cm from the center. The first emergent crown vetch seedling and the first emergent native seedling will be allowed to grow, and all subsequent seedlings will be removed. Control pots with one plant of each species (crown vetch and each native species) growing alone will also be planted. Ten (10) replicate pots for each treatment combination and of each individual species growing alone will be established. Because I am measuring competition as the effect of one individual on another individual, the number of seeds germinating is not important. The only important factor is to have one plant of each species per treatment pot. Greenhouse temperature will be maintained between 10-20 °C and ambient light will be used. Pots will be kept near field capacity with careful and attentive watering. Plants will be allowed to grow for a 5 month growing season after which aboveground biomass will be removed. Biomass from each individual plant will immediately be placed in envelopes, labeled, and placed in a drying oven set at 60 °C until constant weight is achieved. Biomass will then be weighed. Response variables include: 1) biomass of plants growing with and without a neighbor; and 2) a relative interaction index. The relative interaction index that will be used is Rii= (Bw-B0)/(Bw+B0) where Bw is plant biomass produced with competition (growing with a neighbor) and B0 is plant biomass produced without competition (growing alone). This index has strong mathematical properties (i.e., it is linear, has no discontinuities in its range, and is symmetrical around zero) and reflects the competitive ability of crown vetch. Data will be analyzed in JMP Pro 12 (JMP Pro, Version 10. SAS Institute Inc., Cary, NC, 2012).Objective 2: Determine if soil solarization effectively reduces the seedbank of non-native crown vetch (Securigera varia). Solarization is a method of placing plastic film on the soil surface during the summer to control plant invasions. The basic premise is that the heat that accumulates under the solarization treatment during the entire summer kills seeds in the seedbank. Solarization uses minimal labor, does not introduce chemicals into the environment, nor cause large disturbance. This method has been shown to be effective in controlling plant invasions in southwestern rangelands. We will adapt this method as a potential strategy for controlling crown vetch invasion in South Dakota. Because crown vetch is a recent invader to South Dakota, patches that are large and dense enough to adequately replicate our treatments are rare. Therefore, this field experiment will take place at the Murfield Waterfowl Production Area managed by US Fish and Wildlife Service. Crown vetch was first recorded at Murfield in 2014 and has since experienced substantial population growth. In this experiment, the solarization film will be considered the 'treatment', and areas adjacent to and outside the film will be considered the 'control'. Twenty 1m X 1m squares of 60 μm thick black polyethylene film will be deployed on patches of crown vetch at Murfield for an entire summer season. The film will be placed in the field early in the season but after crown vetch plants can be identified. The film will be held in place with landscape fabric staples. We will check the film every 10 days and replace any staples that have been lost and replace any film that has been damaged. Film will be removed late in the season after plants senesce. Seed bank samples will be collected: 1) from both the treated plots and control plots before the film is deployed; 2) from both the treated plots and control plots immediately after the film is removed; and 3) from the control plots later in the season after seed shatter. Seed banks will be examined using methods described in Espeland, Perkins, and Leger, 2010. The objective of this study is to examine if a season-long solarization treatment will eliminate the seed bank of crown vetch, thus the response variable will be crown vetch germination from the seed bank samples. The efficacy of the treatments will be determined by the difference in crown vetch germinants from samples collected from under the treatments and from control areas. Data will be analyzed in JMP Pro 12 (JMP Pro, Version 10. SAS Institute Inc., Cary, NC, 2012).Objective 3: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to a native riparian species. The competitive ability of Russian olive will be determined using cage match methods similar to those for objective 1 with some modification. The invasive species will be Russian olive and the native species could include (Salix exigua, Salix amygdaloides, or Populus deltoids). Large containers will be used (Deepot 60, Steuwe and Sons, Corvallis OR). Each cage match container will be planted with one field-collected cutting of Russian olive and one field-collected cutting of the native species. The cuttings will be placed in the container 10 cm apart. Control pots with one individual of each species growing alone will also be planted. The replication level will be 10. In this experiment, growing with a neighbor will be considered the 'treatment' and plants growing alone will be considered the 'control'. Careful and attentive watering will keep the pots near field capacity. Greenhouse temperature will be maintained between 10-20 °C and ambient light will be used. Plants will be allowed to grow for a 5 month growing season after which aboveground biomass will be removed. Biomass from each individual plant will immediately be placed in bags and labeled. Biomass will then be placed in a drying oven set at 60 °C until constant weight is achieved. Biomass will then be weighed. Response variables include: 1) biomass of plants growing with and without a neighbor; and 2) a relative interaction index. The relative interaction index that will be used is Rii= (Bw-B0)/(Bw+B0) where Bw is plant biomass produced with competition (growing with a neighbor) and B0 is plant biomass produced without competition (growing alone). This index has strong mathematical properties (i.e., it is linear, has no discontinuities in its range, and is symmetrical around zero) and reflects the competitive ability of Russian olive. Data will be analyzed in JMP Pro 12 (JMP Pro, Version 10. SAS Institute Inc., Cary, NC, 2012).

Progress 10/01/19 to 09/30/20

Outputs
Target Audience: Nothing Reported Changes/Problems:The Covid-19 pandemic and a failure in the temperature control system of the greenhouse impacted work during this reporting period. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Objective 1: Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants. The manuscript will be finalized and submitted for peer-review. Objective 2: Determine if solarization effectively reduced the seedbank of non-native crown vetch. The manuscript will be finalized and submitted for peer-review. Objective 3: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species. Work on this goal will begin in the next reporting period.

Impacts
What was accomplished under these goals? Objective 1: Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants. (90% accomplished) The first key step in meeting this objective is to create soil conditioned by crown vetch for the competition experiment. Crown vetch is currently growing in the greenhouse and conditioning soil to be used for the experiment. Briefly, crown vetch seeds were collected in a field at the Murfield Waterfowl Production Area managed by the United States Fish and Wildlife Service, Madison Wetland Management District. The seeds were kept in dry storage until the experiment started (January, 2019). Field collected top-soil was placed in 10 '#7' (6.5 gallon) greenhouse pots. Several crown vetch seeds or Canada Wild Rye (Elymus Canadensis) seeds were added to each pot and the first emergent was allowed to grow. Subsequent emergent seedlings were removed. Pots were kept near field capacity with attentive watering. No supplemental light was used in the greenhouse. Greenhouse temperatures were approximately 12°C at night and 27°C during the day. Several pots were not seeded but otherwise treated the same in order to provide a 'control' soil. After a full year, the soil from each treatment was sampled and nutrient availability and the soil microbial community analyzed. A year was sufficient to allow species-specific changes to the soil to manifest. Soil microbial parameters were significantly different among soils previously occupied by crown vetch, E. canadensis, and the control: AM fungi (F=368 df=2,2 p<0.0001); gram negative bacteria (F=26.78 df=2,2 p=0.001); gram positive bacteria (F=97 df=2,2 p<0.0001), and fungi (F=33.6 df=2,2 p=0.0006). Soil nutrients were significantly different among treatments: nitrate ppm (F=869 df=2,2 p<0.0001); potassium ppm (F=308 df=2,2 p<0.0001); and calcium ppm (F=8.9 df=2,2 p=0.0159). However there were no significant differences in pH, Mg, CEC, or total N. The soil was then homogenized within treatments and re-potted in January 2020. A head-to-head competition experiment was be conducted in the soil conditioned by crown vetch, E. canadensis, and in the control soil. The goal of the head-to-head competition experiment was to determine the competitive ability of crown vetch compared with co-occurring natives (d), and to examine the influence of soil conditioning on competitive ability. Within each competition pot, multiple seeds were seeded in each pot and the first emergent crown vetch seedling and the first emergent native seedling were allowed to grow, and all subsequent seedlings were removed. Ten (10) replicate pots for each treatment combination and of each individual species growing alone were established. Unfortunately, a failure in the temperature control system in the greenhouse, coupled with Covid-19 limiting access to labor and facilities, resulted in many of these plants being lost. However, enough plants survived to examine the effect of soil conditioning on plant performance, but not competitive ability. After the freeze in the greenhouse, we decided to harvest seedling biomass to minimize the risk of losing the plants that were left. Aboveground biomass was collected at the soil surface, dried at 60C for over 48 h, and weighed. Response variables include: 1) aboveground biomass; and 2) a relative interaction index. The relative interaction index that was used is Rii= (Bw-B0)/(Bw+B0) where Bw is plant biomass produced in conditioned soil and B0 is plant biomass produced in the control soil. This index has strong mathematical properties (i.e., it is linear, has no discontinuities in its range, and is symmetrical around zero) and reflects the competitive ability of crown vetch. Soil conditioning significantly affected E. canadensis biomass (F=4.75 df=2,2 and rii (F=7.9 df=1,1 ) but not crown vetch biomass (F=0.059 df=2,2 p=0.94) or rii (F=0.045 df=1,1 p=0.84), nor D. canadensis biomass (F=0.77 df=2,2 p=0.48) or rii (F=0.0022 df=1,1 p=0.96). E. canadensis grew significantly larger in the soil conditioned by crown vetch compared to its own soil and the control soil. Although the outcome of this experiment is disappointing, the results suggest that crown vetch might not create soil conditions that negatively impact other species. Thus restoration of crown vetch invaded sites might not require prior soil remediation. Objective 2: Determine if solarization effectively reduced the seedbank of non-native crown vetch. (80% Accomplished) Field and greenhouse work for this objective are complete. Manuscript preparation is in progress. Solarization panels significantly increased soil temperature (p >0.0001, t=3.87, SE 0.009). Solarization panels also significantly decreased the regrowth of crown vetch the next year. Cover of crown vetch decreased from 85% (SE=1.61) in the control plots to 23% (SE= 2.79) in the solarization plots (p >0.0001, F=383.97, df 1,38). Native plant cover was low (3.8% SE=0.80) and not significantly impacted by solarization panels (p=0.53, F=0.39, df 1,38). The amount of bare ground also was increased by solarization panels from a mean of 3% (SE= 2.3) in the control plots to a mean of 49% (SE= 2.4) in the solarization plots (p >0.0001, F=189.4, df 1,38). These results suggest that solarization panels are a method to decrease crown vetch cover in invaded grasslands. Solarization decreased the cover of crown vetch by approximately 60%. Cover of native plants was low in this study area, but was not impacted by solarization. The only concern might be the amount of bare ground left by the solarization panels. This bare ground could either be an open niche that is vulnerable to subsequent invasion by non-native species or could be an open niche for the establishment of desired vegetation through additional restoration activities. Goal Three: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species. (0% Accomplished) Work on this goal has not begun.

Publications


    Progress 10/01/18 to 09/30/19

    Outputs
    Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Objective 1: Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants. This is an ongoing experiment. After the plants senesce for the winter, soils will be collected from each pot and the soil microbial community and nutrient status will be determined. Then a competition experiment will be run in the soil previously occupied by crown vetch and previously unoccupied (control) soil. An individual native plant and a crown vetch plant will be seeded into each pot. After a three month growing period, aboveground biomass will be determined. This data will be used to evaluate the competitive ability of crown vetch. Objective 2: Determine if solarization effectively reduced the seedbank of non-native crown vetch. The manuscript will be finalized and submitted for peer-review. Objective 3: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species. Work on this goal is not scheduled to begin in the next reporting period.

    Impacts
    What was accomplished under these goals? Objective 1: Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants. (20% accomplished) The first key step in meeting this objective is to create soil conditioned by crown vetch for the competition experiment. Crown vetch is currently growing in the greenhouse and conditioning soil to be used for the experiment. Briefly, crown vetch seeds were collected in a field at the Murfield Waterfowl Production Area managed by the United States Fish and Wildlife Service, Madison Wetland Management District. The seeds were kept in dry storage until the experiment started (January, 2019). Field collected top-soil was placed in 10 '#7' (6.5 gallon) greenhouse pots. Several crown vetch seeds were added to each pot and the first emergent was allowed to grow. Subsequent emergent seedlings were removed. Pots are being kept near field capacity with attentive watering. No supplemental light is being used in the greenhouse. Greenhouse temperatures are approximately 12 °C at night and 27 °C during the day. Several pots were not seeded but otherwise treated the same in order to provide a 'control' soil. After a full growing season, the soil from each pot will be sampled and nutrient availability and the soil microbial community will be determined. A head-to-head competition experiment will be conducted in the soil conditioned by crown vetch and in the control soil.The goal of the head-to-head competition experiment is to determine the competitive ability of crown vetch compared with co-occurring natives (options include Echinacea angustifolia, Dalea purpurea, Monarda fistulosa, Cleome serrulata, Ratibida columnifera, Liatris aspera, Gaillardia aristata, and Aster ericoides). Within each competition pot, multiple seeds of crown vetch will be seeded in the center of the pot and multiple seeds of one native species will be planted 2.5 cm from the center. The first emergent crown vetch seedling and the first emergent native seedling will be allowed to grow, and all subsequent seedlings will be removed. Control pots with one plant of each species (crown vetch and each native species) growing alone will also be planted. Ten (10) replicate pots for each treatment combination and of each individual species growing alone will be established. Because I am measuring competition as the effect of one individual on another individual, the number of seeds germinating is not important. The only important factor is to have one plant of each species per treatment pot. Response variables include: 1) biomass of plants growing with and without a neighbor; and 2) a relative interaction index. The relative interaction index that will be used is Rii= (Bw-B0)/(Bw+B0) where Bw is plant biomass produced with competition (growing with a neighbor) and B0 is plant biomass produced without competition (growing alone). This index has strong mathematical properties (i.e., it is linear, has no discontinuities in its range, and is symmetrical around zero) and reflects the competitive ability of crown vetch. Data will be analyzed in JMP Pro 12 (JMP Pro, Version 10. SAS Institute Inc., Cary, NC, 2012). Objective 2: Determine if solarization effectively reduced the seedbank of non-native crown vetch. (65% Accomplished) Field and greenhouse work for this objective are complete. Manuscript preparation is in progress. Solarization panels significantly increased soil temperature (p >0.0001, t=3.87, SE 0.009). Solarization panels also significantly decreased the regrowth of crown vetch the next year. Cover of crown vetch decreased from 85% (SE=1.61) in the control plots to 23% (SE= 2.79) in the solarization plots (p >0.0001, F=383.97, df 1,38). Native plant cover was low (3.8% SE=0.80) and not significantly impacted by solarization panels (p=0.53, F=0.39, df 1,38). The amount of bare ground also was increased by solarization panels from a mean of 3% (SE= 2.3) in the control plots to a mean of 49% (SE= 2.4) in the solarization plots (p >0.0001, F=189.4, df 1,38). These results suggest that solarization panels are a method to decrease crown vetch cover in invaded grasslands. Solaization decreased the cover of crown vetch by approximately 60%. Cover of native plants was low in this study area, but was not impacted by solarization. The only concern might be the amount of bare ground left by the solarization panels. This bare ground could either be an open niche that is vulnerable to subsequent invasion by non-native species or could be an open niche for the establishment of desired vegetation through additional restoration activities. Goal Three: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species. (0% Accomplished) Work on this goal has not begun.

    Publications


      Progress 10/01/17 to 09/30/18

      Outputs
      Target Audience:Land managers and owners concerned about invasive plants, as well as the scientific community, are the target audience for this research. Efforts to distribute information to the target audience will include peer-review papers and extension/outreach publications. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Objective 1: Determine the competitive ability of non-native crown vetch Securigera varia) compared to co-occurring native plants. This is an ongoing experiment. After the plants senesce for the winter, soils will be collected from each pot and the soil microbial community and nutrient status will be determined. Then a competition experiment will be run in the soil previously occupied by crown vetch and previously unoccupied (control) soil. An individual native plant and a crown vetch plant will be seeded into each pot. After a three month growing period, aboveground biomass will be determined. This data will be used to evaluate the competitive ability of crown vetch. Objective 2: Determine if solarization effectively reduced the seedbank of non-native crown vetch. This data will continue to be collected analyzed. Objective 3: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species. Work on this goal is not scheduled to begin in the next reporting period.

      Impacts
      What was accomplished under these goals? Objective 1: Determine the competitive ability of non-native crown vetch (Securigera varia) compared to co-occurring native plants. (20% accomplished) The first key step in meeting this objective is to create soil conditioned by crown vetch for the competition experiment. Crown vetch is currently growing in the greenhouse and conditioning soil to be used for the experiment. Briefly, crown vetch seeds were collected in the field at the Murfield Waterfowl Production Area managed by the United States Fish and Wildlife Service, Madison Wetland Management District. The seeds were kept in dry storage until the experiment started. Field collected top-soil was placed in 10 '#7' (6.5 gallon) greenhouse pots. Several crown vetch seeds were added to each pot and the first emergent was allowed to grow. Subsequent emergent seedlings were removed. Pots are being kept near field capacity with attentive watering. No supplemental light is being used in the greenhouse. Greenhouse temperatures are approximately 12 °C at night and 27 °C during the day. Several pots were not seeded but otherwise treated the same in order to provide a 'control' soil. After a full growing season, the soil from each pot will be sampled and nutrient availability and the soil microbial community will be determined. A head-to-head competition experiment will be conducted in the soil conditioned by crown vetch and in the control soil.The goal of the head-to-head competition experiment is to determine the competitive ability of crown vetch compared with co-occurring natives (options include Echinacea angustifolia, Dalea purpurea, Monarda fistulosa, Cleome serrulata, Ratibida columnifera, Liatris aspera, Gaillardia aristata, and Aster ericoides). Within each competition pot, multiple seeds of crown vetch will be seeded in the center of the pot and multiple seeds of one native species will be planted 2.5 cm from the center. The first emergent crown vetch seedling and the first emergent native seedling will be allowed to grow, and all subsequent seedlings will be removed. Control pots with one plant of each species (crown vetch and each native species) growing alone will also be planted. Ten (10) replicate pots for each treatment combination and of each individual species growing alone will be established. Because I am measuring competition as the effect of one individual on another individual, the number of seeds germinating is not important. The only important factor is to have one plant of each species per treatment pot. Response variables include: 1) biomass of plants growing with and without a neighbor; and 2) a relative interaction index. The relative interaction index that will be used is Rii= (Bw-B0)/(Bw+B0) where Bw is plant biomass produced with competition (growing with a neighbor) and B0 is plant biomass produced without competition (growing alone). This index has strong mathematical properties (i.e., it is linear, has no discontinuities in its range, and is symmetrical around zero) and reflects the competitive ability of crown vetch. Data will be analyzed in JMP Pro 12 (JMP Pro, Version 10. SAS Institute Inc., Cary, NC, 2012). Objective 2: Determine if solarization effectively reduced the seedbank of non-native crown vetch. (65% Accomplished) Solarization panels were deployed in the field over the summer. Twenty 1m X 1 m squares of 60 μm thick black polyethylene film was placed over mowed patches of crown vetch at Murfield WPA. The solarization panels were held in place with landscape staples. Temperature was recorded inside and outside of four of the solarization panels between July 15 and Oct 29. Temperature was significantly affected by date (p<0.001, t ratio=-104.3_ and location (underneath or outside the panels, p<0.001, t ratio=-13.21), but not by a date*location interaction (p=0.96, t ratio=-.05). Solarization panels increased the soil temperature at 5 cm depth by a mean of 0.6 °C over the entire period. However, the difference in soil temperature is greatest in early September when the temperature under the solarization panels was 6.6 °C greater than outside the panels. As the solarization panels were removed, seed bank samples were collected. Three soil cores (10 cm in diameter and 7 cm deep) were collected from each solarization panel and from an adjacent area that was not covered with a solarization panel. The samples were brought back to the greenhouse for evaluation using methods published in Espeland, Perkins, and Leger 2010. Briefly, samples are spread in trays and kept moist to allow seeds to germinate. Every month, soils are allowed to dry down enough to mix the soil sample. As seeds germinate, they are either identified to species and removed from the sample or they are moved to a pot to grow until they can be identified to species. Too few seedlings have been identified to present preliminary data. Goal Three: Determine the competitive ability of non-native Russian olive (Elaeagnus angustifolia) compared to native riparian species. (0% Accomplished) Work on this goal has not begun.

      Publications