Progress 05/24/17 to 09/30/21
Outputs
Target Audience:Plant scientists; lipid researchers; stress biologists; people interested in crop improvement and sustainability. In the long term, farmers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project involved graduate students, Linah Alkotami, Nicholas Neumann, Yu Song, Bilal Ahmad, Bibek Subedi, Pallikonda Arachchige Dona Bashanee Vinusha Wickramasinghe, Heather Forster, and Zolian Zoong Lwe, and undergraduate students, Gabrielle Phillips, Abigail Parker, Emily Raine, Abdul Naeem, Yibo Liu, Kennedy Ayre, Christian Brautigam, Kyle Thompson, Seth Peery, Adara Warner, Graham Mathews, Gary McIntosh, Cole Wilson, Isaac Doolittle, Maxwell Harman, Kathleen Schuler, Hieu Nguyen, Ashley Panagakis, Lindy Karrer, and Daniel Hemphill. The trainees and students received training through their research involvement, which included working with plants and mass spectrometry. How have the results been disseminated to communities of interest?Welti, Durrett, and Schrick are active classroom teachers, who discuss plant stress, plant lipids, climate change, and interactions between crops and the environments in their role as instructors of Kansas State's biochemistry and biology courses. Dissemination also occurs through scientific meetings and publications. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The Welti, Durrett, and Schrick groups have worked together and with other members of the multistate group to improve our understanding of plants and their lipids, with the goals of improving crop stress resiliency and creating crops with high-yield and high-value seed oils. One of the roadblocks for lipid scientists has been lack of standardized methods of lipid analysis. We developed a mass-spectrometry-based plant lipid analytical method, which is modular. We have made the analytical components available so that the method can be adopted by other groups. Together and in collaboration with others in the multistate project, we have made progress in our understanding of the basic science of how plants respond to environmental stresses and in understanding the function of genes involved in lipid metabolism and regulation of transcription. We have worked toward producing crops plants with specialized high-value fatty acids. In particular, camelina producing low-viscosity oils, suitable for use as biodiesel, was successful in field trials. Objective 1: Improve and extend methods for lipid characterization and measurement. The Welti group has made all reagents and methods available for relatively rapid (8 min/sample) direct-infusion multiple reaction monitoring (MRM) mass spectrometry using a Sciex 6500+ triple quadrupole MS (Fruehan et al., 2018; Shiva et al., 2018; Tamura et al., 2020; Song et al., 2020). This deliverable includes a rapid extraction method, standards (available at cost), mass spectral methods downloadable from the web, and updates to expand the functionality of all software for data processing and analysis. The Schrick lab developed a fluorescent labeling approach to study in situ localization of choline phospholipids in plants (Paper et al., 2018). Data suggest choline phospholipids accumulate preferentially in guard cells. In collaboration with the Lee group they were successful in mass spectrometry imaging of phosphatidylcholines in the epidermis layer of Arabidopsis leaves. A NIFA project of Durrett and Lee (Iowa State University) is underway to study the spatiotemporal development of acetyl-TAG in genetically engineered camelina using mass spectrometry imaging methods developed by the Lee group. Objective 2: Lipid-related metabolism and traits relevant for crop improvement LIPIDS of Crops targeted two aspects of crop production i) the role of lipids in response to environmental factors, that can be translated to genetic approaches to protect yield and ii) the improvement of lipid profile and/or content in crop plants, and implementing what is learned from these activities to design genetic strategies to improve quality of output traits. Welti, Durrett, and Schrick collaborated on characterization of plant genes identified in a lipid profiling screen of Arabidopsis T-DNA mutants in which the complex lipid profiles are significantly different from wild-type plants. The identified genes have functions in fatty acid desaturation, cutin metabolism, and transacylase activities. Welti and Durrett are also characterizing the lipid and gene expression changes in camelina seeds during development and germination. Schrick, Welti, and Wang (Danforth Plant Science Center) described changes which occur in Arabidopsis lipid composition and metabolism under heat stress (Shiva et al., 2020). Welti and Koo (U MO-Columbia) groups are seeking to gain insight on the genetic variation and the impact on the phenotypic outcomes of genes involved in lipid metabolism. Particularly, they are looking at variation that impacts enzyme kinetics during response to wounding and mild freezing stress. Welti and Koo labs also analyzed lipids from Arabidopsis leaf tissues from high vegetative oil. To address protection of yield under abiotic stresses, Narayanan (Clemson U) and Welti labs showed that decreases in the unsaturation levels of plastidic and extra-plastidic glycerolipids in pollen are adaptive outcomes in wheat and peanut exposed to heat stress (Narayanan et al., 2018; Zoong Lwe et al., 2021). With funding from the USDA-NIFA, Narayanan and Welti will characterize heat tolerance of members of a soybean recombinant inbred line population (derived from a genetic cross between a heat-tolerant and a susceptible genotype) and elite soybean genotypes by measuring physiological responses and identify molecular markers associated with heat-induced lipid metabolic changes. The Schrick group has been studying the role of lipids in signaling through identification of steroidogenic acute regulatory protein (StAR)-related lipid transfer (START)-domain transcription factors. In plants START domains are found in master regulators of cell developmental fate. Schrick group is looking at their role in DNA binding, gene regulation, and development of trichomes and other features of the epidermis. Lysophospholipids were identified as binding partners of START-domain transcription factors. One family member was shown to bind a phosphate starvation response element to control the expression of several phospholipid catabolism genes. Her group also has been investigating signaling functions of other lipid metabolites including sterol glucosides (Pook et al., 2017) and flavonoids (Velazhahan et al., 2020). The Roston (U NE-Lincoln) and Schrick labs are investigating the roles of sterols in cold tolerance; data suggests that some sterol mutants have reduced freezing tolerance. Durrett and Allen (Danforth Plant Science Center, USDA) have investigated how changes in gene expression at different stages of seed development affect the partitioning of carbon between lipids, proteins and carbohydrates in soybean seeds. Seed composition was measured in fast neutron mutants containing deletions of specific genes involved in carbohydrate or lipid metabolism (Kambhampati et al. 2019). Isotopic labeling experiments indicated changes in activity of different pathways during late seed development (Kambhampati et al. 2021). Transgenic lines with reduced expression of lipases active in late seed development were generated to improve soybean seed composition. The Durrett group has also investigated the role of the plastid lipase PLIP1 in supplying fatty acid for the triacylglycerol synthesis in developing Arabidopsis seeds. PLIP1 is upregulated in dgat1 seed. Double mutant plip1 dgat1 seeds are green and fail to germinate (Aulakh and Durrett, 2019). Ongoing work is testing the hypothesis that PLIP1 provides a substrate necessary for oil synthesis. Objective 3: Develop crops with improved yield and functionality Acetyl-TAGs are unusual TAG molecules that possess reduced viscosity due to the presence of an sn-3 acetate group. By expressing a high activity DAG acetyltransferase, the Durrett group developed camelina events that produced seeds accumulating over 90 mol% acetyl-TAG with minimal effect on seed size, weight and fatty acid content (Alkotami et al, 2021). To further lower the viscosity of seed oil, the Durrett and Cahoon (U of Nebraska-Lincoln) groups generated camelina plants capable of synthesizing acetyl-TAG with medium-chain fatty acids (MCFA). Field trials of the best transgenic events enabled viscosity testing of the acetyl-TAG derived from these lines (Bansal et al, 2018). In addition, Cahoon and Durrett groups screened a camelina EMS mutant population and identified lines with altered seed fatty acid composition. Sequencing of candidate genes revealed causative mutations in homeologs of FAB1, FAD2, FAD3 and FAE1. Crossing to generate a fae1c/fad2a/fae1a/fad3a quadruple mutant resulted in a mid-oleic oils with nearly 40% oleic acid and reduced very long-chain (≤C20) fatty acid content. The resulting mid-oleic acid oil had improved oxidative stability due to reductions in polyunsaturated fatty acid content, increasing its utility for biofuels and other applications (Neumann et al, 2021). References: Please see Products section.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Kambhampati S, Aznar-Moreno JA, Bailey SR, Arp JJ, Chu KL, Bilyeu KD, Durrett TP, Allen DK. Temporal changes in metabolism late in seed development affect biomass composition. Plant Physiol. 2021 Jun 11;186(2):874-890. doi: 10.1093/plphys/kiab116. PMID: 33693938; PMCID: PMC8195533.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Durrett TP, Welti R. The tail of chlorophyll: Fates for phytol. J Biol Chem. 2021 Jan-Jun;296:100802. doi: 10.1016/j.jbc.2021.100802. Epub 2021 May 19. PMID: 34022219; PMCID: PMC8191296.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Song Y, Zoong Lwe ZS, Wickramasinghe PADBV, Welti R. Head-Group Acylation of Chloroplast Membrane Lipids. Molecules. 2021 Feb 26;26(5):1273. doi: 10.3390/molecules26051273. PMID: 33652855; PMCID: PMC7956594.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Zoong Lwe ZS, Welti R, Anco D, Naveed S, Rustgi S, Narayanan S. Heat stress elicits remodeling in the anther lipidome of peanut. Sci Rep. 2020 Dec 17;10(1):22163. doi: 10.1038/s41598-020-78695-3. PMID: 33335149; PMCID: PMC7747596.
- Type:
Other
Status:
Other
Year Published:
2020
Citation:
Oral presentation: Yu Song. Lipidomics-based GWAS study on Arabidopsis thaliana reveals candidate genes related to lipid metabolism and stress tolerance . Biochemistry and Molecular Biophysics Student Seminar, October 12, 2020.
- Type:
Other
Status:
Other
Year Published:
2021
Citation:
Oral presentation: Yu Song. Lipidomics-based GWAS study on Arabidopsis thaliana reveals candidate genes related to lipid metabolism and stress tolerance . Sciex Midwest Thought Leader Series, May 11, 2021.
- Type:
Other
Status:
Other
Year Published:
2021
Citation:
Oral presentation: Ruth Welti. Direct-infusion Lipidomics at Kansas Lipidomics Research Center . Sciex Midwest Thought Leader Series, May 11, 2021.
- Type:
Other
Status:
Other
Year Published:
2020
Citation:
Oral Presentation: Nicholas Neumann. "Investigating the roles of PLASTID LIPASE1 in oilseed PUFA biosynthesis"; Biochemistry and Molecular Biophysics Student Seminar; November 9, 2020
- Type:
Other
Status:
Other
Year Published:
2021
Citation:
Oral Presentation: Linah Alkotami. "Metabolic engineering strategies to enhance acetyl-TAG synthesis in Camelina sativa"; Biochemistry and Molecular Biophysics Student Seminar; March 8, 2021
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Linah Alkotami, Catherine Kornacki, Shahna Campbell, Gary McIntosh, Cole Wilson, Tam N. T. Tran, and Timothy P. Durrett. (2021) Expression of a high activity diacylglycerol acetyltransferase results in enhanced synthesis of acetyl-TAG in camelina seed oil. The Plant Journal. 106: 953-964. doi.org/10.1111/tpj.15210
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Poster presentation: Bibek Subedi. "Regulator of leaf trichome and root epidermis development is controlled by degradation in plant cells." Research and the State. Capitol Graduate Research Summit. Graduate Student Poster Session (Zoom), Oct. 23, 2020.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2021
Citation:
Poster presentation: Adara Warner. "Characterization of transcriptional coregulators of the homeodomain protein GLABRA2". 19th Annual K-INBRE Symposium: 2021 Virtual K-INBRE Symposium, Jan. 14-15, 2021.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2021
Citation:
Oral presentation: Bibek Subedi. "How does a decorated protein undergo a perilous journey during plant development?" Kansas State University Three Minute Thesis (3MT) Competition (Zoom), Feb. 19, 2021.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2021
Citation:
Oral presentation: Bibek Subedi. "GLABRA2 transcription factor is regulated by ubiquitin-mediated degradation". Virtual KSU Division of Biology Grad Student Forum, April 3, 2021.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2021
Citation:
Oral presentation: Bilal Ahmad. "Nuclear localization of HD-Zip IV transcription factor GLABRA2 is driven by importin alpha". Virtual KSU Division of Biology Grad Student Forum, April 3, 2021.
- Type:
Other
Status:
Other
Year Published:
2021
Citation:
Oral presentation: Bibek Subedi. "Zipper-Loop-Zipper motif mediates targeting of homeodomain transcription factors for degradation." Molecular, Cellular and Developmental Biology (MCDB) Seminar Series, Kansas State University (Zoom), April 8, 2021.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2021
Citation:
Poster presentation: Adara Warner. "Characterization of transcriptional coregulators of homeodomain protein GLABRA". K-State Undergraduate Research Showcase, April 20-22, 2021. https://symposium.foragerone.com/ksurs21/presentations/20614
|
Progress 10/01/19 to 09/30/20
Outputs
Target Audience:Plant scientists; lipid researchers; stress biologists; people interested in crop improvement and sustainability. In the long term, farmers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project involved graduate students, Linah Alkotami, Nicholas Neumann, Yu Song, Bilal Ahmad,Bibek Subedi, and Zolian Zoong-Lwe, and undergraduate students, Gabrielle Phillips, Abigail Parker, Emily Raine, Abdul Naeem, Yibo Liu, Kyle Thompson, Seth Peery, Adara Warner, Graham Mathews, Gary McIntosh, Cole Wilson, Isaac Doolittle and Daniel Hemphill. The trainees and students received training through their research involvement, which included working with plants and mass spectrometry. How have the results been disseminated to communities of interest?Welti, Durrett, and Schrick are active classroom teachers, who discuss plant stress, plant lipids, climate change, and interactions between crops and the environments in their role as instructors of Kansas State's biochemistry and biology courses. Dissemination also occurs through scientific meetings and publications. What do you plan to do during the next reporting period to accomplish the goals?The Welti, Durrett, and Schrick labs will continue working on collaborative projects to characterize novel lipid-related genes from Arabidopsis. In the Schrick lab, the relationship between phospholipid binding of START-transcription factors and the phosphate starvation response will be further investigated. Structure-function analyses of START-transcription factors will be extended to include the study post-translational modifications such as phosphorylation and ubiquitination. The Durrett and Welti labs will complete work on an ongoing investigation on a fatty acid desaturase. The KSU researchers will continue projects with multistate collaborators at University of Nebraska, Donald Danforth Plant Science Center/USDA, and Washington State University on seed oil improvement for crop plants, at Iowa State University to understand how acetyl TAG is localized in camelina during development, at University of Missouri-Columbia to define the function of genes activated during plant wounding, and at the Clemson University on the role of lipids in crop plants under heat stress. We are also excited to continue to work with multistate collaborators to develop new projects.
Impacts
What was accomplished under these goals?
The Welti, Durrett, and Schrick groups have worked together and with other members of the multistate group to improve our understanding of plants and their lipids, with the goals of improving crop stress resiliency and creating crops with high-yield and high-value seed oils. One of the roadblocks for lipid scientists has been lack of standardized methods of lipid analysis. We developed a mass-spectrometry-based plant lipid analytical method, which is modular. We have made the analytical components available so that the method can be adopted by other groups. Together and in collaboration with others in the multistate project, we have made progress in our understanding of the basic science of how plants respond to environmental stresses and in understanding the function of genes involved in lipid metabolism and regulation of transcription. We have worked toward producing crops plants with specialized high-value fatty acids. In particular, camelina producing low-viscosity oils, suitable for use as biodiesel, was successful in field trials. Objective 1: Improve and extend methods for lipid characterization and measurement. The Welti group has made all the components available for relatively rapid (8 min/sample) direct-infusion multiple reaction monitoring (MRM) mass spectrometry (Song et al., 2020). The components include a rapid extraction method (Shiva et al., 2018), which was independently tested by Rebecca Roston's group at the University of Nebraska (Mahboub et al., 2020), standards available at cost, all parameters for MRM (and the data acquisition method downloadable from the web at https://www.k-state.edu/lipid/analytical_laboratory/analysis_components/data_acquisition_methods/index.html ), and a complete data processing method that employs Sciex software and the online site LipidomeDB Data Calculation Environment. They also updated Lipidome DB Data Calculation Environment twice: once to support lipids analysis by multiple reaction monitoring (Fruehan et al., 2018; Song et al., 2020), and once to support collision induced fragment analysis by time-of-flight mass spectrometry; this technique can be used for fatty acid analysis at the chemical formula level (Tamura et al., 2020). A NIFA project of Durrett and Lee (Iowa State University) was recently funded to study the spatiotemporal development of acetyl-TAG in genetically engineered camelina using mass spectrometry imaging methods developed by the Lee group. Objective 2: Lipid-related metabolism and traits relevant for crop improvement Two aspects of crop improvement are studied in this objective: i) the role of lipids in response to environmental factors and ii) the improvement of lipid profile and/or content in crop plants: Welti, Durrett, and Schrick are working together to characterize the function of several plant genes that were identified in a lipid profiling screen of Arabidopsis T-DNA mutants as having complex lipid profiles that are significantly different from wild-type plants. One gene is involved in fatty acid desaturation, one is involved cutin metabolism, and one appears to be a transacylase. The Welti and Schrick groups, along with Xuemin Wang (Donald Danforth Plant Science Center/University of Missouri-St. Louis) recently described the changes that occur in Arabidopsis lipid composition and metabolism in heat stress (Shiva et al., 2020). Welti's lab, in collaboration with Abe Koo's lab (University of Missouri-Columbia), is working to identify genes involved in lipid metabolism that have natural variation affecting the activities of associated gene products. Particularly, they are interested in natural variation that results in altered enzyme activities during response to wounding and mild freezing stress. Pertaining to abiotic stresses, collaborative work between Sruthi Narayanan's lab (Clemson University) and Welti, showed that wheat decreases the unsaturation levels of plastidic and extra-plastidic glycerolipids of leaf and/or pollen in order to adapt to heat stress (Narayanan et al., 2016a, 2016b, 2018). Similar lipid changes contribute to high-temperature adaptation in both leaves and pollen, though the lipidomes have distinct compositions. Narayanan and Welti also investigated if lipid metabolic changes mediate heat stress responses in soybean (Narayanan et al., 2020). The Schrick group has been studying the role of lipids in signaling through identification of START-domain transcription factors and their role in DNA binding, gene regulation and development of trichomes and other features of the epidermis. Phospholipids and lysophospholipids have been identified as putative binding partners of these transcription factors. One of the family members has been shown to bind a phosphate starvation response element to control the expression of several phospholipid catabolism genes. Additionally, the Schrick has been investigating signaling functions of other lipid metabolites including sterol glucosides (Pook et al., 2017) and flavonoids (Velazhahan et al., 2020). Schrick has started a new collaboration with Rebecca Roston (University of Nebraska-Lincoln) to investigate the roles of sterols in cold tolerance. Durrett and Doug Allen (Donald Danforth Plant Science Center/USDA) have been collaborating to produce soybeans with altered lipid levels by reducing the expression of lipases that are upregulated late in development. Work over the past year focused on obtaining transformants and growing to homozygosity with phenotypic measurements of biomass components including lipid. Biomass from available mutants of a fast neutron population was assessed through stages in development (Kambhampati et al. 2020). Changes in the lipid, protein and oligosaccharides may correlate with genes and indicate promising avenues for carbon partitioning in seeds. This work will continue in the new funding cycle of the LIPIDS in Crops grant, including further assessment of transgenics related to lipid, protein, and carbohydrate metabolism aimed at producing high value soybeans. The Durrett group has investigated the role of the plastid lipase PLIP1 in supplying FA for the synthesis of TAG in developing Arabidopsis seeds. PLIP1 is upregulated in dgat1 seed. Double mutant plip1 dgat1 seed are green and fail to germinate (Aulakh and Durrett, 2019). Ongoing work is testing the hypothesis that PLIP1 provides a PUFA-substrate pool necessary for the function of PDAT1. Objective 3: Develop crops with improved yield and functionality Acetyl-TAGs are unusual TAG molecules that possess reduced viscosity due to the presence of an sn-3 acetate group. By expressing a high activity DAG acetyltransferase, the Durrett group developed camelina lines that produced seeds containing over 90 mol% acetyl-TAG with minimal effect on seed size, weight and fatty acid content. However, a slight (2 day) delay was observed in germination. To further lower the viscosity of seed oil, the Durrett group, in collaboration with the Cahoon group, generated camelina plants capable of synthesizing acetyl-TAG containing medium-chain fatty acids (MCFA). Field trials of the best transgenic lines enabled viscosity testing of the acetyl-TAG derived from these lines (Bansal et al, 2018). In addition, the Durrett group and Ed Cahoon's group (University of Nebraska-Lincoln) screened a camelina EMS mutant population and identified lines with altered seed fatty acid composition. Sequencing of candidate genes revealed causative mutations in homeologs of FAB1, FAD2, FAD3 and FAE1. Crossing to generate a fae1c/fad2a/fae1a/fad3a quadruple mutant resulted in a mid-oleic oils with nearly 40% oleic acid and reduced very long-chain (≤C20) fatty acid content. The resulting mid-oleic acid oil had improved oxidative stability due to reductions in polyunsaturated fatty acid content, increasing its utility for biofuels and other applications.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2020
Citation:
Song Y, Vu HS, Shiva S, Fruehan C, Roth MR, Tamura P, Welti R. 2020.A lipidomic approach to identify cold-induced changes in Arabidopsis membrane lipid composition. Editors: D. Hincha and E. Zuther. Methods Mol Biol 2156, 187-202. doi: 10.1007/978-1-0716-0660-5_14.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
McGinn M, Phippen WB, Chopra R, Bansal S, Jarvis BA, Phippen ME, Dorn KM, Esfahanian M, Nazarenus TJ, Cahoon EB, Durrett TP, Marks MD, Sedbrook JC (2019) Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop. Plant Biotechnol J 17, 776-788
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Mukherjee T, Lerma-Reyes R, Thompson KA, Schrick K. 2019. Making glue from seeds and gums: Working with plant-based polymers to introduce students to plant biochemistry. Biochem Mol Biol Educ 47, 468-475. DOI:10.1002/bmb.21252
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Velazhahan V, Glaza P, Herrera AI, Prakash O, Zolkiewski M, Geisbrecht BV, Schrick K. Dietary flavonoid fisetin binds human SUMO1 and blocks sumoylation of p53. PLoS One. 15(6), e0234468. doi: 10.1371/journal.pone.0234468.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Shiva S, Samarakoon T, Lowe KA, Roach C, Vu HS, Colter M, Porras H, Hwang C, Roth MR, Tamura P, Li M, Schrick K, Shah J, Wang X, Wang H, Welti R. Leaf lipid alterations in response to heat stress of Arabidopsis thaliana. Plants (Basel). 9(7):845. doi: 10.3390/plants9070845.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Narayanan S, Zoong-Lwe ZS, Gandhi N, Welti R, Fallen B, Smith JR, Rustgi S. Comparative lipidomic analysis reveals heat stress responses of two soybean genotypes differing in temperature sensitivity. Plants (Basel). 9(4), 457. doi: 10.3390/plants9040457.
- Type:
Book Chapters
Status:
Awaiting Publication
Year Published:
2021
Citation:
Tamura P, Fruehan C, Johnson DA, Hinkes P, Williams TD, Welti R. (2021) Fatty acid composition by total-acyl-lipid--collision induced dissociation--time-of-flight (TAL-CID-TOF) mass spectrometry. Editors: D. Bartels and P. Doermann. Methods Mol Biol. In press.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Poster presentation: Cole Wilson and Timothy Durrett. A Method for characterizing membrane-bound proteins. 18th Annual K-INBRE Symposium, Wichita, KS. January 17-19, 2020
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Poster presentation: Adara Warner and Kathrin Schrick. Identification of regulatory domains in HD-Zip IV transcription factor GLABRA2. 18th Annual K-INBRE Symposium, Hyatt Regency Wichita, Wichita, KS, Jan. 17-19, 2020.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Linah Alkotami. Enhancing acetyl-TAG synthesis through metabolic engineering of the oilseed crop Camelina sativa. BMB Graduate Student Seminar. 2 December 2019.
- Type:
Other
Status:
Other
Year Published:
2020
Citation:
Oral presentation: Kathrin Schrick. Plant Molecular Biology, Epidermal Development and Gene Expression. Short Presentation, Feb. 21, 2020, Division of Biology, Kansas State University
- Type:
Other
Status:
Other
Year Published:
2020
Citation:
Oral presentation: Bilal Ahmad. Nuclear localization of HD-Zip transcription factor GL2. Short Presentation, Grad Students on Parade, MCDB Seminar, Sept. 10, 2020, Kansas State University.
- Type:
Other
Status:
Other
Year Published:
2020
Citation:
Oral presentation: Bibek Subedi. Post-translational modifications control the stability of HD-Zip IV transcription factors. Short Presentation, Grad Students on Parade, MCDB Seminar, Sept. 10, 2020, Kansas State University.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Yu Song. Lipidomics-based GWAS study on Arabidopsis thaliana reveals two candidate genes (LOH2 and KCS9), which are important for sphingolipid metabolism and freezing tolerance. Seminar, Department of Biochemistry and Molecular Biophysics, Kansas State University. December 9, 2019.
- Type:
Other
Status:
Other
Year Published:
2020
Citation:
Oral presentation: Yu Song. Lipidomics-based GWAS study on Arabidopsis thaliana reveals candidate genes related to lipid metabolism in response to abiotic stresses. Talk to Angelovicci lab at University of Missouri-Columbia. January 14, 2020.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Poster presentation: Seth Peery and Kathrin Schrick. HD-Zip transcription factors Key regulators of development and metabolism. 18th Annual K-INBRE Symposium, Hyatt Regency Wichita, Wichita, KS, Jan. 17-19, 2020.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster presentation: Sruthi Narayanan, Sachin Rustgi, Zolian S. Zoong Lwe, Ruth Welti. Lipid metabolic changes contribute to heat tolerance in soybean. Crop Science Society of America. Nov. 10-13, 2019. San Antonio, TX.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Ruth Welti, Plants, Lipids, and Stress. Seminar. University of Missouri-St. Louis. October 29, 2019.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Thiya Mukherjee. HD-Zip IV START domain regulates transcription factor activity in an HD-dependent manner. Seminar. MCDB Seminar Series, Kansas State University, Nov. 22, 2019.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Nicholas Neumann. Characterizing the role of PLASTID LIPASE 1 in developing Arabidopsis seeds. BMB Graduate Student Seminar. 11 November 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Oral presentation: Yu Song. Lipidomics-based GWAS study on Arabidopsis thaliana reveals candidate genes related to lipid metabolism and stress tolerance. Zoom talk to the CROPS group, organized by graduate students/postdocs at the University of Nebraska for plant science students/postdocs in Nebraska and surrounding states. August 20, 2020.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Kambhampati, S, Aznar-Moreno, JA, Hostetler, C, Caso, T, Bailey, SR, Hubbard, AH, Durrett, TP, Allen, DK. 2020. On the inverse correlation of protein and oil: Examining the effects of altered central carbon metabolism on seed composition using soybean fast neutron mutants. Metabolites 10, 18.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Tamagno, S, Aznar-Moreno, JA, Durrett, TP, Prasad, PVV, Rotundo, JL, Ciampitti, IA. 2020. Dynamics of oil and fatty acid accumulation during seed development in historical soybean varieties. Field Crops Research 248, 107719
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2021
Citation:
Neumann, NG, Nazarenus, TJ, Aznar-Moreno, JA, Rodriguez-Aponte, SA, Mejias Veintidos, VA, Comai, L, Durrett, TP, Cahoon, EB. 2021. Generation of camelina mid-oleic acid seed oil by identification and stacking of fatty acid biosynthetic mutants. Industrial Crops and Products 159, 113074. doi: 10.1016/j.indcrop.2020.113074
|
Progress 10/01/18 to 09/30/19
Outputs
Target Audience:Plant scientists; lipid researchers; stress biologists; people interested in crop improvement and sustainability. In the long term, farmers.Plant scientists; lipid researchers; stress biologists; people interested in crop improvement and sustainability. In the long term, farmers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project involved postdoctoral trainee, Thiya Mukherjee, graduate students, Linah Alkotami, Nicholas Neumann, Hannah Lusk, Yu Song, Ruben Lerma-Reyes, and Bilal Ahmad,and undergraduate students, Gabrielle Phillips, Abigail Parker, Emily Raine, Abdul Naeem, Yibo Liu, Kyle Thompson, Seth Peery, Adara Warner, Graham Mathews, Gary McIntosh, Cole Wilson, Isaac Doolittle and Daniel Hemphill. The trainees and students received training through their research involvement, which included working with plants and mass spectrometry. How have the results been disseminated to communities of interest?Welti, Durrett, and Schrick are active classroom teachers, who discuss plant stress, plant lipids, climate change, and interactions between crops and the environments in their role as instructors of Kansas State's biochemistry and biology courses. Dissemination also occurs through scientific meetings and publications. What do you plan to do during the next reporting period to accomplish the goals?The Welti, Durrett, and Schrick labs will continue working on collaborative projects to characterize novel lipid-related genes from Arabidopsis. In the Schrick lab, the relationship between phospholipid binding of START-transcription factors and the phosphate starvation response will be further investigated. Structure-function analyses of START-transcription factors will be extended to include the study post-translational modifications such as phosphorylation and ubiquitination. The Durrett lab will express variants of desaturase enzymes in yeast to characterize the effect of different mutations identified by the Welti lab.
Impacts
What was accomplished under these goals?
Objective 1: Improve and extend methods for lipid characterization and measurement. The Welti group has worked on making available all components needed to perform direct-infusion multiple reaction monitoring (MRM) lipid analysis using a Sciex 6500+ triple quadrupole mass spectromter. The components include a rapid extraction method, standards available at cost, all parameters for MRM (and the data acquisition method downloadable from the web), and a complete data processing method that employs Sciex software and the online site LipidomeDB Data Calculation Environment. They have written a paper pointing users to all components. They are also updating Lipidome DB Data Calculation Environment to support collision induced fragment analysis by time-of-flight mass spectrometry; this technique can be used for fatty acid analysis at the chemical formula level. Welti, Durrett, and Jonathan Markham of University of Nebraska-Lincoln are beginning a project to develop a lipid mass map (visualization of fragments vs. intact ion m/z) which will be used to analyze data from plants across the plant kingdom. The data will be stored in a database of complex lipid compositions. Objective 2: Lipid-related metabolism and traits relevant for crop improvement Welti, Durrett, and Schrick are working together to characterize the function of several plant genes that were identified in a lipid profiling screen of Arabidopsis T-DNA mutants as having complex lipid profiles that are significantly different from wild-type plants. One gene is involved in fatty acid desaturation, one is involved cutin metabolism, and one seems to be involved in lysophospholipid acylation. Welti's lab is also working to identify genes involved in lipid metabolism that have natural variation affecting the activities of associated gene products. Particularly, they are interested in natural variation that results in altered enzyme activities during response to wounding and mild freezing stress. Some of the lipid analyses have been done in collaboration with Abe Koo of the University of Missouri-Columbia. Pertaining to abiotic stresses, the Kutty (Narayanan) lab at Clemson, in collaboration with Welti, showed that wheat decreases the unsaturation levels of plastidic and extra-plastidic glycerolipids of leaf and/or pollen in order to adapt to heat stress. Lipid analysis of leaves of multiple soy lines under heat stress are underway. The Schrick group has been studying the role of lipids in signaling through identification of START-domain transcription factors and their role in DNA binding, gene regulation and development of trichomes and other features of the epidermis. Phospholipids have been identified as putative binding partners of these transcription factors. One of the family members has been identified to bind a phosphate starvation response element to negatively regulate several phospholipid catabolism genes. Other aspects of START-domain transcription factors are being investigated, including subcellular localization, transcriptional activation, and post-translational modifications. A project of Durrett lab and Doug Allen at the Danforth Plant Science Center includes investigations in soybean metabolism to understand the partitioning of oil late in development into other storage reserves. The Durrett group has also been exploring the role of the lipase PLIP1 in supplying FA for the synthesis of TAG in developing Arabidopsis seeds. PLIP1 is upregulated in dgat1 seed. Double mutant plip1 dgat1 seed are green and fail to germinate. Ongoing work is testing the hypothesis that PLIP1 provides a PUFA-substrate pool necessary for the function of PDAT1. Objective 3: Develop crops with improved yield and functionality The Durrett group has been studying the germination of seeds that produce high levels of acetyl-TAGs. Synthetic biology approaches are being used to alter the timing of Euonymus fortunei diacylglycerol acetyltransferase to increase levels of accumulation in seeds; other projects aim to incorporate other fatty acids (e.g., ricinoleic acid) into acetyl-TAG molecules to further alter properties.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Aulakh K, and Durrett TP. 2019. The plastid lipase PLIP1 is critical for seed viability in diacylglycerol acyltransferase 1 mutant seed. Plant Physiol 180, 1962-1974. doi: 10.1104/pp.19.00600
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Fruehan C, Johnson D, Welti R. 2018. LipidomeDB Data Calculation Environment has been updated to process direct-infusion multiple reaction monitoring data. Lipids 53, 1019-1020. doi: 10.1002/lipd.12111
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Obeng E, Obour AK, Nelson NO, Aznar-Moreno JA, Ciampitti IA, Wang D and Durrett TP. 2019. Seed yield and oil quality as affected by Camelina cultivar and planting date. J Crop Improv. 33, 202-222. https://doi.org/10.1080/15427528.2019.1566186
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Wang K, Durrett TP, and Benning C. 2019. Functional diversity of glycerolipid acylhydrolases in plant metabolism and physiology. Prog Lipid Res. 75, 100987, https://doi.org/10.1016/j.plipres.2019.100987
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Ruth Welti, Using lipidomics to understand plant response to the environment, University of South Dakota, March 25, 2019.
- Type:
Other
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Ruth Welti, Using lipidomics to understand plant response to the environment, Seminar at the Danforth Plant Science Center, St. Louis, MO, October 17, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Oral presentation: Ruth Welti. Lipid Atlas. Gordon Conference on Plant Lipids. Galveston, TX. January 27-Feb. 1, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Oral Presentation: Thiya Mukherjee and Kathrin Schrick. Plant HD-Zip transcription factors drive epidermal cell fate via lipid-binding START domain. Gordon Research Conference on Plant Lipids, Galveston, TX, Jan. 29, 2019.
- Type:
Other
Status:
Other
Year Published:
2018
Citation:
Oral Presentation: Thiya Mukherjee. HD-Zip proteins drive epidermal fate via lipid-binding START domains, Seminar in the MCDB Seminar Series, Kansas State University, Oct. 29, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Poster Presentation: Ruben Lerma-Reyes, Thiya Mukherjee, and Kathrin Schrick. Identification of a nuclear localization sequence in HD-ZIP transcription factor GLABRA2. oSTEM's 8th Annual Conference: Making Space for Everyone. Westin Galleria Houston, Houston, TX, Nov. 15-18, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Oral Presentation: Thiya Mukherjee, Mentor: Kathrin Schrick, Homeodomain proteins linking lipid metabolism to gene expression in plants. . Developmental Research Project (DRP) Core Meeting, 17th Annual K-INBRE Symposium, Overland Park Sheraton, Overland Park, KS, Jan. 18, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster Presentation: Seth Peery and Kathrin Schrick. HD-Zip Transcription Factors - An Evolutionary Engine. 17th Annual K-INBRE Symposium, Overland Park Sheraton, Overland Park, KS, Jan. 19-20, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster Presentation: Ruben Lerma-Reyes, Thiya Mukherjee, and Kathrin Schrick. Identification of a nuclear localization sequence in HD-ZIP transcription factor GLABRA2, a candidate lipid-binding protein. Gordon Research Conference on Plant Lipids, Galveston, TX, Jan. 27-Feb.1, 2019.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral Presentation: Thiya Mukherjee. Understanding the roles of biopolymers: from regulating gene expression to metabolism and host-pathogen interaction. Seminar at the Danforth Plant Science Center, St. Louis, MO, March 29, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Oral Presentation: Kathrin Schrick, Sterol Composition is Critical for Protein Partitioning in the Plasma Membrane. Sterols symposium, featuring the Schroepfer Medal Award lecture, AOCS Meeting, Americas Convention Center Complex, St. Louis, MO, May 6, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster Presentation: Seth Peery and Kathrin Schrick. HD-Zip Transcription Factors - An Evolutionary Engine. 2019 IDeA Central Region Conference COBRE, INBRE, Sheraton Hotel, Oklahoma City, OK, June 12-14, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster Presentation: Cole Wilson and Timothy P. Durrett. Subdomain shuffling in two similar acyltransferases to investigate acyl specificity. 17th Annual K-INBRE Symposium, Overland Park Sheraton, Overland Park, KS, Jan. 19-20, 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster Presentation: Linah Alkotami, Catherine Kornacki, Tam N. T. Tran, Shahna Campbell, and Timothy P. Durrett. Enhancing acetyl-TAG synthesis through metabolic engineering of the oilseed crop Camelina sativa. Gordon Research Conference on Plant Lipids, Galveston, TX, Jan. 27-Feb.1, 2019.
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
Oral Presentation: Timothy P. Durrett. Remodeling oilseed biochemistry to synthesize novel lipids with enhanced properties. Seminar at the Donald Danforth Plant Sciences Center, St Louis, MO. 6 March 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Poster Presentation: Linah Alkotami and Timothy P. Durrett. Enhancing acetyl-TAG synthesis through metabolic engineering of the oilseed crop Camelina sativa. 2019 American Oil Chemists Society Annual Meeting, Saint Louis, MO. May 5- 8, 2019.
|
Progress 10/01/17 to 09/30/18
Outputs
Target Audience:Plant scientists; lipid researchers; people interested in crop improvement and sustainability. In the long term, farmers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? In 2018, the project has involved postdoctoral trainees, Sunitha Shiva, Jose A. Aznar-Moreno, and Thiya Mukherjee, graduate students, Linah Alkotami, Nicholas Neumann, Ruben Lerma-Reyes, Hannah Lusk, and Yu Song, and undergraduate students, Cora Farley, Gabrielle Phillips, Yibo Liu, Maggie Malmberg, Seth Peery, Kyle Thompson, Kyler Weingartner, Gary McIntosh, Cole Wilson and Daniel Hemphill. The trainees and students received training through their research involvement, which included working with plants and quantifying lipid composition using mass spectrometry or gas chromatography. How have the results been disseminated to communities of interest? Welti, Durrett, and Schrick are active classroom teachers, who discuss plant stress, plant lipids, climate change, and interactions between crops and the environments in their role as instructors of Kansas State's biochemistry and biology courses. Dissemination also occurs through scientific meetings and publications. What do you plan to do during the next reporting period to accomplish the goals? We plan to continue to develop analytical methods for plant polar lipids and triacylglycerols. In particular, Welti is working the Jonathan Markham of University of Nebraska to complete comprehensive characterization of the sorghum leaf and seed lipidomes. Welti's group will also continue its work to discover and verify candidate genes involved in stress response via genome wide association and mutant analysis of candidates. Schrick will continue to work with the Lee group at Iowa State University to develop an imaging method to detect lipids in the epidermis. Work in the Schrick lab will also continue to characterize START-domain containing transcription factors and their link to lipid signaling. Structure-function studies are underway to characterize DNA binding, dimerization and turnover of these key regulatory proteins. Additionally, various lipid binding assays are being optimized in conjunction with mass spectrometry methods to discover candidate ligands. The Durrett group will express additional acyltransferase genes from castor, along with genes responsible for acetyl-TAG and ricinoleic acid synthesis in order to increase the accumulation of acetyl-TAG molecules contain hydroxy fatty acids.
Impacts
What was accomplished under these goals?
Objective 1: Improve and extend methods for lipid characterization and measurement. A new Sciex 6500+ triple quadrupole MS with differential ion mobility spectrometry, funded by an NSF MRI grant is installed at the Kansas Lipidomics Research Center (KLRC) and is functional; Ruth Welti and Tim Durrett have been trained on the instrument. The Welti lab has developed and published an abbreviated lipid extraction method, tested on Arabidopsis and sorghum leaves (Shiva et al., 2018). The group has also reduced the time required for direct-infusion MRM analysis of ~370 lipids from 30 min to ~9 min. Polar lipid internal standard mixes are now available through the KLRC. The Schrick lab developed and published a fluorescent labeling approach to determine the subcellular localization of choline phospholipids in plants (Paper et al., 2018). Data suggest choline phospholipids accumulate preferentially in guard cells. Schrick and Lee were successful in MS imaging of phosphatidylcholines (PCs) in the epidermis of Arabidopsis leaves. Schrick received a Big 12 Faculty Fellowship to support this collaborative research. LipidomeDB Data Calculation Environment, online program for processing direct-infusion mass spectrometry-based lipidomics data, was updated in summer 2018. The update includes improved ability to edit compounds and fragments, and, most importantly, ability to process MRM data with or without differential ion mobility. Processing includes isotopic deconvolution and normalization to internal standards. Objective 2: Lipid-related metabolism and traits relevant for crop improvement The Schrick group has been studying the role of lipids in signaling through identification of START-domain transcription factors and their role in DNA binding, gene regulation and development of trichomes and other features of the epidermis. They have identified the nuclear localization signal for these transcription factors. In collaboration with Doug Allen of the USDA at Danforth Plant Science Center, Tim Durrett has identified soybean fast neutron mutant lines to reduce oligosaccharides and increase oil. Welti lab is using genome wide association studies to identify genes responsible for lipid metabolism that occurs in response to wounding and freezing stresses. In collaboration with Ed Cahoon (University of Nebraska), the Durrett group has identified mutations in lipid biosynthetic genes in camelina mutant lines with altered oil composition. Objective 3: Develop crops with improved yield and functionality The Durrett group has developed transgenic camelina lines that produce over 90% acetyl-TAG. These lines have been crossed with lines producing medium-chain fatty acids or ricinoleic acid to further alter the properties of acetyl-TAGs. In collaboration with Toni Wang of Iowa State University, the viscosity and cold-temperature properties of blends of acetyl-TAG and regular TAG were quantified.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Narayanan S, Prasad PVV, Welti R. 2018. Alterations in wheat pollen lipidome during high day and night temperature stress. Plant Cell Environ. 41, 1749-1761. doi: 10.1111/pce.13156.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Djanaguiraman M, Boyle DL, Welti R, Jagadish SVK, and Prasad PVV. 2018. Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation, oxidation, acylation, and damage of organelles. BMC Plant Biol. 18, 55. doi: 10.1186/s12870-018-1263-z.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Djanaguiraman M, Perumal R, Jagadish SVK, Ciampitti IA, Welti R, and Prasad PVV. 2018. Sensitivity of sorghum pollen and pistil to high-temperature stress. Plant Cell Environ. 41, 1065-1082. doi: 10.1111/pce.13089.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Paper JM, Mukherjee T, and Schrick K. 2018. Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants. Plant Methods 14, 31.
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2018
Citation:
McGinn M, Phippen WB, Chopra R, Bansal S, Jarvis BA, Phippen ME, Dorn KM, Esfahanian M, Nazarenus TJ, Cahoon EB, Durrett TP, Marks MD, Sedbrook JC. 2018. Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop. Plant Biotechnology Journal. In press.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Bansal S, Kim HJ, Na G, Hamilton ME, Cahoon EB, Lu C, Durrett TP. 2018. Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids. Journal of Experimental Botany. 69, 4395-4402.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Ruth Welti, "Roles for lipids in plant responses to abiotic stress", Gordon Research Conference on Salt and Water Stress in Plants, Waterville Valley, NH, June 3-8, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Ruth Welti, "Using lipidomics to understand plant response to the environment", Seminar in Department of Chemistry, Wichita State University, February 14, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Ruth Welti, "Using lipidomics to understand plant response to the environment", Keynote talk at Midwest Regional Meeting of the American Society of Plant Biologists, Iowa State University, March 3-4, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Thiya Mukherjee, "A homeodomain transcription factor, its START domain, and epidermal development in plants". Seminar. MCDB Seminar Series, Kansas State University, Manhattan, Kansas, Nov. 6, 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Kathrin Schrick, "Intregrating Lipid Metabolism and Regulation of Gene Expression in Plants". Invited Seminar. Department of Biochemistry, Texas A&M University, College Station, TX, Nov. 7, 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Kyler A. Weingartner and Kathrin Schrick, Understanding the Metabolic Flux of Rhamnose in the Plant Cell , Invited Oral Presentation, American Society of Plant Biologists (ASPB) Midwest Meeting, Howe Hall, Iowa State University, Ames, Iowa, March 3-4, 2018. (Outstanding Undergraduate Oral Presentation Award)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Thiya Mukherjee and Kathrin Schrick, A homeodomain transcription factor, its START domain, and epidermal development in plants , Invited Oral Presentation. American Society of Plant Biologists (ASPB) Midwest Meeting, Howe Hall, Iowa State University, Ames, Iowa, March 3-4, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Kathrin Schrick, "Plant development and role of START lipid/sterol binding domains in homeodomain transcription factors", Invited Seminar, Max Planck Institute of Molecular Plant Physiology, Golm, Germany, May 30, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Kyler A. Weingartner and Kathrin Schrick, Metabolic flux of rhamnose in Arabidopsis , Invited Talk. NIH, NIGMS Seventh Biennial National IDeA Symposium of Biomedical Research Excellence, June 24-26, 2018, Wardman Park Marriott, Washington, D.C.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Kathrin Schrick, "Plant development and START lipid/sterol binding domains in homeodomain transcription factors", Invited Seminar. University of Hohenheim, Stuttgart, Germany, July 12, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Jose Aznar-Moreno and Timothy Durrett, CRISPR-Cas9 genome editing to alter oil Production in the hexaploid oilseed crop. Invited oral presentation. 2018 American Oil Chemists Society Annual Meeting, Minneapolis, MN. April 30 - May 3, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Oral presentation: Timothy Durrett. Remodeling oilseed biochemistry to synthesize novel lipids with enhance properties , Seminar. Department of Biochemistry and Molecular Biophysics seminar series, Kansas State University, Manhattan, KS. September 5, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Poster presentation: Jose Aznar-Moreno and Timothy Durrett. Genome editing and the synthesis of novel oils in Camelina sativa. Bioengineering: Synthetic Circuits to Sustainable Agriculture, Donald Danforth Plant Science Center, Saint Louis, MO. September 26-28, 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Poster presentation: Nicholas Neumann, Chaofu Lu, and Timothy Durrett. Harnessing synthetic biology to produce hydroxy acetyl-TAGs in transgenic Camelina sativa . Bioengineering: Synthetic Circuits to Sustainable Agriculture, Donald Danforth Plant Science Center, Saint Louis, MO. September 26-28, 2018.
|
Progress 05/24/17 to 09/30/17
Outputs
Target Audience:Plant scientists; lipid researchers; stress biologists; people interested in crop improvement and sustainability. In the long term, farmers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project involves postdoctoral trainees, Sunitha Shiva, Jose A. Aznar-Moreno, and Thiya Mukherjee, graduate students, Linah Alkotami, Nicholas Neumann, Hannah Lusk, and Yu Song,and undergraduate students, Cora Farley, Gabrielle Phillips, Yibo Liu, Kyle Thompson and Daniel Hemphill. The trainees and students received training through their research involvement, which included working with plants and mass spectrometry. How have the results been disseminated to communities of interest?Welti, Durrett, and Schrick are active classroom teachers, who discuss plant stress, plant lipids, climate change, and interactions between crops and the environments in their role as instructors of Kansas State's biochemistry and biology courses. Dissemination also occurs through scientific meetings and publications. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue to develop analytical methods for plant polar lipids and triacylglycerols. In particular, we complete development of an integrated approach to speed up lipid analysis while maintaining compound coverage and sensitivity. Mass-spectrometry based methods will be used to discover novel protein-metabolite interactions. This work may lead to new functions for lipids in plant growth and development. We plan to use improved CRISPR-Cas9 vectors to improve the efficiency of genome editing in Camelina sativa. We will explore different strategies such as over-expression of citrate lyase or the elimination of fatty acid elongation to determine whether increased acetyl-CoA levels will lead to enhanced acetyl-TAG synthesis in transgenic camelina lines.
Impacts
What was accomplished under these goals?
1. Improve and extend methods for lipid characterization and measurement. Welti lab has developed a less labor-intensive and more efficient extraction method (Shiva et al., Plant Methods, 2018). Welti, Durrett, Schrick, and others, successfully obtained an NSF-MRI grant to purchase a new triple quadrupole mass spectrometer with ion mobility spectrometry dedicated for the analysis of plant lipids. The mass spectrometer has been acquired, and Welti and Durrett, along with lab members, have taken training on the new instrument. Rapid analytical strategies are being optimized on the mass spectrometer. An analytical method specific to sorghum covering glycerolipids including oxidized lipids and other stress-induced lipids also has been developed. To meet a milestone of having common standards available, KLRC has standard mixes of glycerolipids available http://www.k-state.edu/lipid/analytical_laboratory/prices/index.html The Schrick lab is collaborating with the Young-Jin Lee lab at Iowa State University to develop mass spectrometry imaging of lipids in epidermal cells. Schrick received a Big12 Faculty Fellowship to fund visits to ISU during 2017-18. 2. Identify and characterize lipid-related metabolism and traits relevant for crop improvement. The Schrick lab further studies the role of lipids in signaling through identification and characterization of START-domain containing transcription factors and their role in DNA binding, gene regulation, and development of trichomes and other features of the epidermis. The Cahoon and Durrett groups screened a camelina mutant population for lines containing altered profiles of fatty acids or neutral glycerolipid molecular species in mature seeds. Using a candidate gene sequencing strategy, mutations in at least one of the camelina homeologues of FAD2, FAD3, FAE1 and KASII were identified that were likely responsible for the observed lipid phenotypes in different lines. 3. Develop crops with improved yield and/or functionality. The Durrett lab is studying the accumulation of transgenic enzymes responsible for the synthesis of acetyl-triacylglycerols (acetyl-TAG) during seed development. In addition, the Durrett lab is working to test the chemical properties of oils containing different levels of acetyl-TAG. The Durrett group also used CRISPR/Cas-mediated genome editing to induce mutations in key camelina lipid biosynthetic genes and alter the fatty acid composition in seed.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Shiva, S., Enninful, R., Roth, M.R., Tamura, P., Jagadish, K., and Welti, R. (2018) An efficient modified method for plant leaf lipid extraction results in improved recovery of phosphatidic acid. Plant Methods. 14, 14, doi.org/10.1186/s13007-018-0282-y
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Aznar-Moreno, J.A., and Durrett, T.P. (2017) Simultaneous targeting of multiple gene homeologs to alter seed oil production in Camelina sativa. Plant Cell Physiol. 58, 1260 - 1267.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Pook, V., Nair, M., Ryu, K., Arpin, J.C., Schiefelbein, J., Schrick, K., and DeBolt, S. (2017) Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots. Sci Rep. 7: 5714. doi:10.1038/s41598-017-05925-6
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Timothy P. Durrett. Synthetic biology to engineer novel oils with enhanced properties . 2017 American Oil Chemists Society Annual Meeting, April 2017, Orlando, FL.
- Type:
Other
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Timothy P. Durrett. From Burning Bushes to Chocolate Vines: Using Sequence Diversity to Explore MBOAT Substrate Specificity and Increase Production of Low Viscosity Oil . Departmental seminar in the Department of Chemistry and Biochemistry, University of Southern Mississippi. 1 September 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Timothy P. Durrett. From Burning Bushes to Chocolate Vines: Using Sequence Diversity to Explore MBOAT Substrate Specificity and Increase Production of Low Viscosity Oil . Departmental seminar in the Department Biochemistry, University of Alberta. 28 September 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Poster presentation: Jose A. Aznar-Moreno and Timothy P. Durrett. Using genome editing to alter oil production in Camelina sativa. Predictive Crop Design: Genome-to-Phenome. Lincoln, NE. April 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Poster presentation: Kristen Wilbeck, Sunitha Shiva, Goutham Neravetla, Yibo Liu, Hannah Lusk, Madeline Colter, Pamela Tamura, Mary Roth, Kathrin Schrick, Timothy P. Durrett, and Ruth Welti. "A T-DNA insertion in At5g41900 affects Arabidopsis thaliana growth and lipid composition". Phytochemical Society of North America Meeting 2017. University of Missouri, Columbia, MO, Aug. 5-9, 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Ruth Welti. Using lipid analysis by mass spectrometry to understand plant response to the environment. Phytochemical Society of North America Meeting 2017. University of Missouri, Columbia, MO, Aug. 5-9, 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Ruth Welti. Using lipidomics to probe plant lipid metabolism during abiotic stresses. Predictive Crop Design: Genome-to-Phenome. Lincoln, NE. April 2017.
- Type:
Other
Status:
Other
Year Published:
2017
Citation:
Oral presentation: Ruth Welti. Using lipidomics to probe plant lipid metabolism. Seminar at Rutgers University. April 2017.
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