Progress 07/01/17 to 06/30/20
Outputs
Target Audience:Plant physiologists working on stomata, water loss from plants, temperature responses of plants, and global climate modelers. Changes/Problems:This original goal of this project was to examine stomatal dynamics in response to normal air and helium-air. This goal proved impossible to achieve for technical reasons. However, during the initial experiments of the project, differences in stomatal dynamics were observed between red and blue light illuminated leaves. The remainder of the project focused on these differences. What opportunities for training and professional development has the project provided?The undergraduate students who worked on the project received extensive training and experience in the equipment used to measure stomatal kinetics in an intact leaf. They received extensive training and experience in constructing computer models of dynamic systems. How have the results been disseminated to communities of interest?Two manuscripts are in preparation. The first describes the observed spatial dynamics of stomata in response to red and blue light and presents an extensive mathematical analysis of the data. The second presents a mathematical model of interacting stomata and shows that it is capable of reproducing many aspects of the observed dynamics. What do you plan to do during the next reporting period to accomplish the goals?This is the final report. There is no more work planned.
Impacts
What was accomplished under these goals?
Over the course of this project we have completed an extensive set of experiments to examine the role of blue and red light in determining stomatal dynamics in intact leaves. We have used fourier analyses and spatial statistics to characterize and analyze the data. We have also constructed a spatially-explicit model of stomatal dynamics based on physiologically relevant interactions among neighboring stomata. The model is capable of reproducing many aspects of the complex behavior observed in the empirical measurements.
Publications
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Progress 10/01/18 to 09/30/19
Outputs
Target Audience:Plant physiologists working on stomata, water loss from plants, temperature responses of plants, and global climate modelers. Changes/Problems:The initial plan of work was to use helium oxygen air to investigate stomatal responses to humidity. That approach did not work out, but the data allowed us to identify interesting differences in kinetics using red, blue, and white light. What opportunities for training and professional development has the project provided?The undergraduate student working on the project has received extensive training and experience in the equipment used to measure stomatal kinetics in an intact leaf. He has also received extensive training and experience in constructing the computer model of stomatal kinetics. How have the results been disseminated to communities of interest?One manuscript is in preparation and we expect to produce another manuscript in the future describing the model and it's application to our experimental findings. What do you plan to do during the next reporting period to accomplish the goals?During the remaining 6 months of the project we will finish a manuscript based on the data for stomatal kinetics and we will continue our work on the computer model.
Impacts
What was accomplished under these goals?
During the past year we have completed an extensive set of experiments to investigate the role of blue and red light on stomatal kinetics. We have also analyzed the data using fourier transforms and spatial statistics. A manuscript describing the results is in preparation. We have also made good progress on a computer model that will allow us to investigate several hypotheses concerning the mechanisms for the responses that we have seen in the data for blue and red light above. The progress on this model so far has been very encouraging.
Publications
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Progress 10/01/17 to 09/30/18
Outputs
Target Audience:Plant physiologists working on stomata, water loss from plants, temperature responses of plants, and global climate modelers. Changes/Problems:The initial plan of work was to use helium oxygen air to investigate stomatal responses to humidity. That approach did not work out, but the data allowed us to identify interesting differences in kinetics using red and white light. During the coming year we plan to investigate the role of small differences in stomatal density in causing the differences in kinetics we observed. What opportunities for training and professional development has the project provided?The undergraduate responsible of conducting the experiments left the lab in August. His replacement has been trained to perform the experiments, and has been working on techniques to continue the research next year. How have the results been disseminated to communities of interest?We have begun writing a manuscript based on the data acquired during the last year. Two manuscripts from previous years were published during this year. What do you plan to do during the next reporting period to accomplish the goals?During the coming year, we plan to investigate the role of small differences in stomatal density on differences in kinetics that we observed in our experiments during the past year.
Impacts
What was accomplished under these goals?
Over the past year, we completed initial experiments with helium oxygen air to investigate kinetics of stomatal responses to humidity. Unfortunately, these initial experiments were sufficient to show us that our approach would not work. However, the data did suggest differences in kinetics between red and white light, and we have pursued that avenue of investigation for most of the year. We have completed one aspect of this investigation and have begun writing a manuscript based on the results.
Publications
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Progress 07/01/17 to 09/30/17
Outputs
Target Audience:Plant physiologists working on stomata, water loss from plants, temperature responses of plants, and global climate modelers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The undergraduate who will conduct the experiments has been trained on the equipment and has developed the analysis programs necessary to perform the experiments. How have the results been disseminated to communities of interest?There are no results to disseminate yet. What do you plan to do during the next reporting period to accomplish the goals?We plan to immediately initiate experiments on the time course for stomatal responses to humidity. Our ability to track small areas of the leaf independently will allow a much more precise measurement than has been possible in previous studies using gas-exchange techniques. Once we are confident of these data, we will begin experiments using helium:oxygen air to look for differences in kinetics.
Impacts
What was accomplished under these goals?
Over the past 6 months we have finished setting up and testing the thermal camera and gas-exchange system for kinetic experiments. We have also developed software programs to allow rapid processing of large image data files. We have performed several preliminary experiments to test the accuracy of our system.
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