Source: PURDUE UNIVERSITY submitted to NRP
FACILITATING THE REGENERATION AND RESTORATION OF NATIVE HARDWOOD FOREST TREES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1012964
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2017
Project End Date
Sep 30, 2022
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
PURDUE UNIVERSITY
(N/A)
WEST LAFAYETTE,IN 47907
Performing Department
Forestry & Natural Resources
Non Technical Summary
Hardwood forests throughout the United States provide important ecological and economic benefits. With increasing population growth, however, there is tremendous pressure on these forests to supply timber, clean water, wildlife habitat, and recreation. The nation will increasingly turn toward plantations to help meet wood product needs. Concurrently, there is increasing interest in using plantations to help restore hardwood forest habitats for conservation purposes. Artificial forest regeneration through tree planting offers a means to control species composition, incorporate genetically improved (e.g., disease-resistant) stock, and apply intensive culture that may lead to enhanced tree growth and wood quality compared to natural forests. Additionally, tree plantations may aid in restoration of degraded areas (e.g., mine reclamation sites) or reintroduction of threatened native species, such as American chestnut (Jacobs et al. 2013). While extensive plantation research has been conducted with conifer species of the western and southeastern United States, regeneration issues in hardwood forests have received relatively little attention (Wilson and Jacobs 2006; Jacobs 2011).Establishment success of hardwood tree species is highly variable and factors such as herbivory, drought or nutrient stress, and vegetative competition result in many examples of failed plantations. In Indiana (Central Hardwood Region), for instance, survival of operationally planted hardwoods averages about 66%, with less than half of surviving seedlings deemed free-to-grow after five years (Jacobs et al. 2004). In other hardwood zones in the United States, lack of availability of appropriate hardwood nursery stock (i.e., species and stock quality) and pre- and post-planting management techniques limit operational regeneration success. This contrasts with conifer plantations in the western and southeastern United States, where survival is frequently above 90% and indicates the need for improvements in hardwood regeneration productivity.The Hardwood Tree Improvement and Regeneration Center (www.htirc.org) at Purdue University was founded nearly 20 years ago and has demonstrated that silvicultural to improve the quality of nursery seedlings, using appropriate planting techniques, and applying after-planting care to juvenile trees may significantly improve seedling establishment success and early productivity of hardwood regeneration (Jacobs 2011). Additionally, the Tropical Hardwood Tree Improvement and Regeneration Center (www.trophtirc.org) was established in 2010 to address overlapping priorities specific to Pacific Island hardwood tree species.Despite these strides, new threats are constantly emerging due to global change (e.g., invasive species, pests and pathogens, and a warmer climate; Jacobs et al. 2015). There is also an increased emphasis on restoration of degraded sites and conversion of abandoned conifer plantations to native hardwoods. This implicates the shifting and evolving nature of challenges that must be overcome to ensure forest productivity in hardwood-dominated forests.Thus, the basic function of this research project is to improve the regeneration of hardwood forests by identifying factors that limit successful forest regeneration and by formulating new silvicultural technologies to help overcome these constraints. We focus efforts on three important hardwood zones of the United States: the temperate Central Hardwood Forest Region, sub-temperate maritime forests along the southeastern Atlantic Coast, and tropical Hawaii. The outcomes of this endeavor promise to be of substantial economic and ecological benefit to the residents and environment of these regions.
Animal Health Component
70%
Research Effort Categories
Basic
30%
Applied
70%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1230620106034%
1230621106033%
1230640106033%
Goals / Objectives
The primary objective of this project is to enhance the regeneration and restoration success of native hardwood forest tree species in three important hardwood zones of the United States: the temperate Central Hardwood Forest Region, sub-temperate maritime forests along the southeastern Atlantic Coast, and tropical Hawaii. This will be addressed by improving knowledge of site-specific biotic and abiotic factors that limit establishment and juvenile development of hardwood forest regeneration. Simultaneously, management practices that promote hardwood plantation survival and growth will be identified and communicated. The specific project objectives are to:Evaluate influences of overstory light availability on eco-physiological responses of hardwood regeneration during site restoration.Determine the relative influence of animal browse on establishment of hardwood regeneration and identify cost-effective management options to control damage.Assess influences of competing vegetation control and nursery cultural treatments on establishment success of hardwood regeneration in afforestation and reforestation sites.Examine physiological and growth responses of hardwood regeneration to varying forms and application rates of nitrogen fertilizers.
Project Methods
To evaluate influences of overstory light availability on eco-physiological responses of hardwood regeneration during site restoration, we will evaluate complementary trials in three regions of study. In the temperate Central Hardwood Forest Region, we will evaluate conversion of introduced white pine (Pinus strobus) to native species including American chestnut (Castanea dentata) and northern red oak (Quercus rubra). These species are of high economic and ecological value in this region, yet are threatened by disease (chestnut) or poor regeneration in natural hardwood stands (oak). In sub-temperate maritime forests along the southeastern Atlantic Coast in Georgia, we will collaborate with a scientist at New Mexico State University to examine conversion of abandoned loblolly pine (Pinus taeda) plantations to the historically dominant maritime forests by planting live oak (Quercus virginia). Finally, in tropical Hawaii, we will collaborate with scientists at the University of Hawaii at Manoa to study conversion of the introduced Japanese tsugi pine (Cryptomeria japonica) to native koa (Acacia koa). In each of these regions, our overall objective is to evaluate effects of canopy shading on the morphological and physiological development of hardwood regeneration in order to aid species restoration. This will be accomplished by reducing overstory density to specific levels of residual tree basal area (white pine, loblolly pine; using a randomized complete block design with minimum 4 replications) or by incorporating existing patchiness (due to scattered mortality) that has created high variability in overstory tree density (tsugi pine; using a completely randomized design). We will measure survival and growth responses in all experiments but also evaluate physiological development of planted hardwood regeneration by measuring leaf water potential, gas exchange, and nutrient uptake. These results should help to provide important insight into the mechanisms controlling growth responses under the varying residual canopy overstory light conditions.We will use experimental trials in the three regions of study to determine the relative influence of animal browse on establishment of hardwood regeneration and to identify cost-effective management options to minimize this damage. Browsing damage, primarily from deer in all three regions of study, may be controlled by fencing but its use is limited in operation due to relatively high costs (Jacobs et al. 2004). Thus, experiments will be established to evaluate the relative efficiency of fencing, other direct forms of browse protection (e.g., plastic tree shelters or repellents), and techniques designed to allow escape from deer browse through promotion of rapid free-to-grow status of seedlings above the level of browse (e.g., field fertilization). We will plant live oak in Georgia and koa in Hawaii, while in Indiana the focus species will be oaks (Quercus spp.) and black walnut (Juglans nigra). Each experiment will be established as a randomized complete block design (minimum 4 replications). Field measurements including survival and growth will be recorded at the end of at least two growing seasons in each the study; level of browsing intensity will also be assessed during early-spring and late-fall. We will combine seedling responses in these studies with a report of estimated costs associated with the various alternative options to fencing. Collectively, these results will provide an assessment of the relative efficacy of varying browse control methods and serve as a guide to land managers in choosing options to best meet their objectives.Chemical site preparation and/or release treatments with herbicides are often used to promote establishment of native hardwoods, yet the potential for herbicide phytotoxicity is not well understood. Thus, to assess influences of competing vegetation control and nursery cultural treatments on establishment success of hardwood regeneration in afforestation and reforestation sites, we will install field experiments in each of the three study regions. The trials will include weed control (Georgia, Hawaii, Indiana) and/or use of high quality nursery stock (Hawaii, Indiana). Similar to objective two, we will focus on live oak in Georgia and koa in Hawaii, while in Indiana we will evaluate oaks and black walnut (Juglans nigra). Weed control treatments in all three study areas will consist of an untreated control, 1 year of weed control, or 2 years of weed control with herbicide (i.e., glyphosate) at recommended application rates. In Hawaii and Indiana, we will implement a factorial design with weed control treatments and varying nursery stocktypes (e.g., container vs. bareroot seedlings in Indiana; different container sizes and/or hardening regimes in Hawaii). Each experiment will be established as a randomized complete block design (minimum 4 replications). Field measurements including survival and growth will be recorded at the end of at least two growing seasons in each the study. Leaf water potential and nitrogen content will also be measured to quantify water stress and plant nutrient availability associated with weed control and/or nursery stocktypes. At the peak of vegetation cover (August), vegetation surveys will be conducted to evaluate competition. Through workshops, field days, and publications, these results will be communicated directly to land managers in each of the regions, providing relevant important to inform hardwood reforestation and restoration operations.To examine physiological and growth responses of hardwood regeneration to varying forms and application rates of nitrogen (N) fertilizers, we will establish controlled environment trials using black walnut in Indiana. Using the rare stable isotope 15N, these studies will determine relative efficiency of N uptake (i.e., Salifu et al. 2008, 2009b) from different N forms applied at varying phenological phases. This will be accomplished by installing factorial experiments with one-year-old black walnut seedlings transplanted into containers in a greenhouse and treated with four independent variables: N form (nitrate, ammonium, glycine), N concentration (3mM, and 12mM), and N form rate (equimolar, nitrate dominant, ammonium dominant and glycine dominant) at two different phenological stages (early spring and mid to late-summer). Nitrogen fertilizers will be labeled as 15N solutions to allow for direct quantification of uptake rates among the varying N treatments. Seedlings will be periodically evaluated for photosynthesis rates and chlorophyll content during active growth and destructively harvested at the end of the growing season. Seedlings will be divided into new stems and leaves, stems, coarse roots, and fine roots. These plant organs will each be analyzed for total N concentrations, and 15N/14N isotopic ratios, which will allow for determination of relative contributions of N uptake from soil vs. retranslocation of N from reserves in new growth of black walnut seedlings. Collectively, these results will allow for evaluation of how differences in N demand, photosynthetic rate, or internal N status may explain variation in N uptake rates within treatments and seasons. This research will also allow us to determine plant performance in response to varying N forms and potential interactions with soil N availability. Results from these experiments will be synthesized to provide recommendations for management systems that ensure the efficient use of high cost N fertilizer.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience: Scientists, Nursery managers; Forestry professionals; Natural resource land managers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Two MS students, one PhD student, one post-doctoral scientist, and one undergraduate student received training under the umbrella of this project. How have the results been disseminated to communities of interest?In 2020, we disseminated our research through oral presentations at the NSF I/UCRC Center for Advanced Forestry Systems annual research meeting. Additionally, we presented results from this project at the Hardwood Tree Improvement and Regeneration Center Advisory Committee Meeting (West Lafayette, Indiana) andthe Tropical Hardwood Tree Improvement and Regeneration Center Advisory Committee Meeting (Honolulu, Hawaii). What do you plan to do during the next reporting period to accomplish the goals?The following describes plans in the next reporting period for our project in relation to the stated project objectives. 1) We will continue working with a former MS student (Jennifer Lesko) topublish anotherpaper (complementingLesko and Jacobs 2018,New Forests)on conversion of white pine plantations to native hardwoods. We will also maintain and re-evaluate the established study(following Thyroff et al. 2019,Frontiers in Plant Science) on overstory canopy effects on live oak restoration. 2) We will develop an extension publication based on the results presented in Redick and Jacobs (2020,Forests). We also plan to pursue a cost analysis of the various deer browse management methods in order to identify cost-benefit options for landowners establishing forests. 3) We have established a new study to examine effectiveness of nursery cultural treatments in determining field establishment success of native sandalwood trees for restoration in Hawaii. 4) We will continue to work to publish manuscripts related to our past studies of fertilization and drought effects in black walnut. Additionally, we will continue to disseminate results from all project objectives through presentations and workshops at scientific and technical venues.

Impacts
What was accomplished under these goals? The following describes outputs for the project period in relation to the stated project objectives over the thirdyear of the project: 1) We published a paper (Rose et al. 2020,Agricultural and Forest Meteorology) from the dissertation of a PhD student (Kyle Rose) describing the effects of light availability on the growth and development ofkoa (Acacia koa) treesunderplanted into established sugi pine plantationsduring restoration in Hawaii. 2) We published a paper (Redick et al. 2020,Forest Ecology and Management) on the effects of deer browsing on hardwood plantation success across Indiana. Management treatments were relatively ineffective in the presence of deer browsing. We also published a review article (Redick and Jacobs 2020,Forests) on the effectiveness of deer browse control methods on hardwood plantations, confirming that fencing is a superior option.3)We published a paper (Schempf and Jacobs 2020,Forests) from a former MS student (Weston Schempf) that examined interactions between nursery stocktypes and vegetation control on hardwood regeneration in southern Indiana. We also published a 10-year study (Jacobs et al. 2020,Forests) of koa in Hawaii, demonstrating the importance of nursery cultural treatments in determining plantation establishment success. 4) We published a nursery study (Mariotti et al. 2020,Forests) examining the use of different fertilizer and media treatments to produce high quality seedlings of several oak (Quercus) species for reforestation.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Rose KME, Friday JB, Oliet JA, Jacobs DF. 2020. Canopy openness affects microclimate and performance of underplanted trees in restoration of high-elevation tropical pasturelands. Agricultural and Forest Meteorology 292293:108105.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Redick CH, McKenna JR, Carlson DE, Jenkins, MA, Jacobs DF. 2020. Silviculture at establishment of hardwood plantations is relatively ineffective in the presence of deer browsing. Forest Ecology and Management 474:118339.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Redick CH, Jacobs DF. 2020. Mitigation of deer herbivory in temperate hardwood forest regeneration: a systematic review of research literature. Forests 11:1220.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Mariotti B, Martini S, Raddi S, Tani A, Jacobs DF, Oliet, JA, Maltoni, A. 2020. Coconut coir as a sustainable nursery growing media for seedling production of ecologically diverse Quercus species. Forests 11:522.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Shempf WM, Jacobs DF. 2020. Hardwood species show wide variability in response to silviculture during reclamation of coal mine sites. Forests 11:72.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Jacobs DF. Davis AS, Dumroese RK, Burney, OT. 2020. Nursery cultural techniques facilitate restoration of Acacia koa competing with invasive grass in a dry tropical forest. Forests 11:1124.


Progress 10/01/18 to 09/30/19

Outputs
Target Audience: Scientists, Nursery managers; Forestry professionals; Natural resource land managers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Three MS students, two post-doctoral scientists, and one undergraduate student received training under the umbrella of this project. How have the results been disseminated to communities of interest? We have disseminated our research through oral presentations at the University of Georgia (invited keynote at annual research symposium - School of Forest Resources), the University College Dublin (invited seminar - School of Agriculture and Food Science)in Ireland, and at the Indiana Society of Mining and Reclamation Technology Transfer Seminar in Evansville, Indiana. We also presented our research at a field dayon maritime forest restoration on St Simon's Island,Georgia. Additionally, in 2019we presented results from this project at the Hardwood Tree Improvement and Regeneration Center Advisory Committee Meeting (West Lafayette, Indiana) andthe Tropical Hardwood Tree Improvement and Regeneration Center Advisory Committee Meeting (Hilo, Hawaii).? What do you plan to do during the next reporting period to accomplish the goals? The following describes plans in the next reporting period for our project in relation to the stated project objectives. 1) We will work with a former MS student (Jennifer Lesko) topublish anotherpaper (complementingLesko and Jacobs 2018,New Forests)on conversion of white pine plantations to native hardwoods. We will continue following the established studyon overstory canopy effects on live oak restoration (following Thyroff et al. 2019,Frontiers in Plant Science). 2) We will maintain our newly established studyto examine alternative forms of browse protection (tree tubes) and use of field fertilization to promote rapid height growth, which may serve as more cost-effective forms of browse control relative to fencing. A graduatestudent (Caleb Redick) that is studying impacts of deer browse on temperate hardwood regeneration will defend hisMSthesis and work to publish his papers. 3) We will publish a paper from a former MS student (Weston Schempf) that examined interactions between nursery stocktypes and vegetation control on hardwood regeneration in southern Indiana. 4) We will work to publish the manuscripts related to the study of fertilization and drought effects in black walnut as described above. Additionally, we will continue to disseminate results from all project objectives through presentations and workshops at scientific and technical venues.?

Impacts
What was accomplished under these goals? The following describes outputs for the project period in relation to the stated project objectives over the secondyear of the project: 1) We published a paper (Thyroff et al. 2019,Frontiers in Plant Science) describing the conversion of loblolly pine forestry plantationsto native live oak (Quercus virginiana)maritime hardwoodforestsin coastal Georgia. In this study, we explored the effect of different overstory tree canopy treatments on light availability in the understory and interactions with vegetation control on performance ofplanted trees. Planted live oak trees performed best under the clearcut treatment, and vegetation control was only beneficial to seedling performance in the clearcut. We also published two papers (Rose et al. 2019,Annals of Botany; Rose et al. 2019,Forest Ecology and Management)from the dissertation of a PhD student (Kyle Rose) describing effects of light availability in canopy gaps on plasticity of phenotype and heteroblasty inrestoration of koa (Acacia koa) trees in Hawaii.2) We published a paper (Thyroff et al. 2019,Forests)describing a trial toexamine live oak tree regeneration in response to deer exclusion (by fencing). Live oak was heavily damaged by deer when not fenced; with fencing, live oak grew 1-2 m in two years. Additionally, we installed a new study in coastal Georgia to examine lower cost alternatives to fencing for controlling deer browse on planted live oaks, including use of plastic tree shelters and plastic mesh tubes. 3)We published a paper (Thyroff et al. 2019,Forests)describing a trial toexamine live oak regeneration in response toweed control (and interactions with deer browse)for live oak regeneration in coastal Georgia. A MS student (Emily Thyroff)defended her thesis examining effects ofdeer browsing, vegetation control, and overstory tree canopy dynamics on live oak regeneration in coastal Georgia.4) A post-doctoral scientist (Mercedes Uscola) produced drafts of publications from her researchof nitrogen form on uptake and re-translocation of nitrogen in black walnut seedlings. She also studied effects of drought on fertilization responses, finding no interactions between drought and fertilization on photosynthesis rates. We also published a paper (Gauthier and Jacobs 2019,Canadian Journal of Forest Research) describing black walnut drought interaction responses.?

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Gauthier MM, Jacobs DF. 2019. Photosynthetic parameters of Juglans nigra trees are linked to cumulative water stress. Canadian Journal of Forest Research 49:752758
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: L�f M, Madsen P, Metslaid M, Witzell J, Jacobs DF. 2019. Restoring forests: regeneration and ecosystem function for the future. New Forests 50:139-152.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Rose KME, Mickelbart MV, Jacobs DF. 2019. Plasticity of phenotype and heteroblasty in contrasting populations of Acacia koa. Annals of Botany 124:399-409.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Rose KME, Friday JB, Jacobs DF. 2019. Canopy openness drives heteroblasty in contrasting Acacia koa populations. Forest Ecology and Management 453:117592.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Thyroff EC, Burney OT, Jacobs DF. 2019. Herbivory and competing vegetation interact as site limiting factors in maritime forest restoration. Forests 10, 950.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2019 Citation: Thyroff EC, Burney OT, Mickelbart MV, Jacobs DF. 2019. Unraveling shade tolerance and plasticity of semi-evergreen oaks: insights from maritime forest live oak restoration. Frontiers in Plant Science doi: 10.3389/fpls.2019.01526


Progress 10/01/17 to 09/30/18

Outputs
Target Audience: Scientists, Nursery managers; Forestry professionals; Natural resource land managers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Three MSstudents, two post-doctoral scientists, and one undergraduate studentreceived training under the umbrella of this project. How have the results been disseminated to communities of interest?Through oral presentations at the Morton Arboretum (Chicago, IL) and the University of Alberta, Canada. We also helped to coordinate a workshop on maritime forest restoration for managers of barrier islands in Georgia, which was held at theGeorgia DNR Coastal Regional Headquarters inBrunswick, Georgia. Additionally, in 2018 we presented these results at theHardwood Tree Improvement and Regeneration Center Advisory Committee Meeting (West Lafayette, IN) and theTropical Hardwood Tree Improvement and Regeneration Center Advisory Committee Meeting (Hilo, HI). What do you plan to do during the next reporting period to accomplish the goals?The following describes plans in the next reporting period for the project in relation to the stated project objectives 1) publish a second paper on conversion of white pine plantations to native hardwoods per theM.S. thesis work described above. The MS student working on overstory canopy effects on live oak restoration will defend and deposit her MS thesis. We will then prepare to publish her manuscripts. 2) Publish a paper from a MS student that explored importance of fencing for browse protection of live oak regeneration. We will also install a new trial to examine alternative forms of browse protection (tree tubes) and use of field fertilization to promote rapid height growth, which mayserve as more cost-effective forms of browse control relative to fencing. 3) We will publish two papers from a MS student that showed the importance of weed control (and interactions with fencing / overstory tree density) on live oak regeneration. We will also publish a paper from a MS student that examined interactions between nursery stocktypes and vegetation control on hardwood regeneration in southern Indiana. 4) We will work to publish the manuscripts related to the study of fertilization and drought effects in black walnutas described above.Additionally, we will continue to disseminate results from all project objectivesthrough presentations and workshops at scientific and technical venues.

Impacts
What was accomplished under these goals? The following describes outputs for the project period in relation to the stated project objectives over the first year of the project:1) We published a paper (Lesko and Jacobs 2018)describing the conversion of introduced white pineto native hardwood species including American chestnut and northern red oak. In this study, we explored the effect of overstory tree canopy on light availability in the understory and interactions with field fertilization of planted trees. We found that the clearcut or partial harvest of the overstory produced similar, positive results on hardwood regeneration. Fertilization was only beneficial in high light environments. We also installed a new study to examine the influence of overstory tree canopyon live oak regeneration (and interactions with vegetation control) in coastal Georgia. 2) Wepublished a paper (Burney and Jacobs 2018)describing how susceptibiity of hardwood regeneration to deer browse is species specific - black cherry was more likely to be browsed than oaks. We also installed a new trial to examine live oak regeneration in response to fencing to exclude deer- live oak was heavily damaged by deer when not fenced; with fencing, live oak grew 1-2 m in two years. 3) We installed a series of trials to show the importance of weed control for live oak regeneration in coastal Georgia. Additionally, a MS student defended his thesis after studying the importance of nursery stocktypes and vegetation control on hardwood regeneration in southern Indiana on mine reclamation sites. 4) A post-doctoral scientist completed her trials exploring the importance of nitogen form on uptake and re-transolcation of nitrogen in black walnut seedlings. She also studied effects of drought on fertilization responses, finding no interactions between drought and fertilization on photosynthesis rates.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Burney OT, Jacobs DF. 2018. Species selection  a fundamental silvicultural tool to promote forest regeneration under high animal browsing pressure. Forest Ecology and Management 408:67-74.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Gauthier MM, Jacobs DF. 2018. Ecophysiological drivers of hardwood plantation diameter growth under non-limiting light conditions. Forest Ecology and Management 419-420:220-226.?
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Lesko J, Jacobs DF. 2018. Conversion of introduced conifer plantations to native hardwoods: Influences of light and nutrients on understory regeneration. New Forests 49:829-849.