Progress 05/08/17 to 09/30/21
Outputs
Target Audience:Environmental health department employees, dairy animal care workers, water quality professionals, drinking water treatment plant and facilities operators. Changes/Problems:None What opportunities for training and professional development has the project provided?Five undergraduates and two PhD students from PI Dr. Zhou's research laboratory in the Department of Biological Engineering were supported. In addition, two PhD students from our collaborators' research groups in the Department of Animal Diary and Veterinary Science joined our collaboration. How have the results been disseminated to communities of interest?Since 2017, the research finding from this project was presented to the undergraduate course BENG 1800 (Introduction to Undergrad Research) with 90~100+ freshmen engineering students at USU. The students involved in this project have presented their research results at regional and national conferences, such as Annual Conference of the Institute of Biological Engineering (IBE) and the Annual Intermountain Biological Engineering Conference (IBEC). PI Zhou's research laboratory has hosted a number of high school students through USU outreach programs: Biotech Summer Academy (annual June, host 2~3 high school students in PI Zhou's laboratory), Engineering State (annual July, 250-300 local high school students), Capitol on the Hill. By attending annual multi-state project meeting, PI Zhou presented the research findings and shared with other researchers in this multi-state project from other land-grand universities and explored potential research collaborations. In addition, PI Zhou contacted Utah Department of Environmental Quality Division Water Quality and Bear River Health Department to learn the waterborne pathogen Cryptosporidium outbreak information in Utah and their current methods to detect the waterborne pathogens, and compared to the performance of our developed Cryptosporidium biosensor technology. What do you plan to do during the next reporting period to accomplish the goals?On Oct 1st, 2021, this multi-state project started another 5-year performance period. We will extend our nanotechnology and biosensor in agriculture, animal science, and environment monitoring applications. Especially, we will apply our biosensor instrument and technology to screen antioxidants cytoprotective effect and monitor plant physiology during growth under external stimuli, e.g., abiotic and biotic stressors.
Impacts
What was accomplished under these goals?
The major technical accomplishments in this multi-state project are associated with the following objectives: Objective #1: Develop new technologies for characterizing fundamental nanoscale processes. Nanoscale DNA-DNA hybridization and detection has been realized by the assessment of the DNA-mediated electron transfer related electrochemical behaviors using a variety of electrochemical techniques, such as cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy. Quantitative measurements of DNA-mediated electrochemical responses are fully characterized which provides direct information to optimize design of the prototype sensor device. As another side project, we collaborated with the faculty members Prof. Heloisa Rutigliano and Prof. Irina Polejaeva from USU Department of Animal Diary and Veterinary Science (ADVS) to characterize their Placenta-derived extracellular vesicles (EVs) samples which typical size is ranging from 100-1000 nm, using Raman spectroscopy. See Publication#3 below and other 2 related conference presentations. Objective #2: Construct and characterize self-assembled nanostructures. To construct specific DNA biosensor device, the fabricated sensor gold surface has been optimized for step-by-step DNA immobilization and hybridization. The overall performance factors such as sensitivity, selectivity, detection limit, and linear range was evaluated accordingly in each of steps of self-assembled gold nanoparticles with the subsequent immobilization of probe DNA, target DNA, and hybridization of probe--target sequences binding have been thoroughly investigated. The nanoparticle deposition conditions (e.g., time, concentration, pH, buffer, etc.) have been also studied in order to obtain desirable sensor responses. Objective #3: Develop devices and systems incorporating microfabrication and nanotechnology. A microfluidic device hosted the patterned three-electrode layout for electrochemical detection of Cryptosporidium DNA sequence has been prototyped and tested. See Publication#1 below. In addition, PI Zhou joined a collaboration with a group of researchers from 15 land-grand universities supported by this multi-state project to publish a peer reviewed article on Biosensor research in Food, Environment, Agriculture, Science, Technology in North America. See Publicaiton#2 below. PI Zhou also published two papers on the use of machine learning analysis of Raman spectroscopy data from diesel exhaust particles included oxidative stress damage cells and the protective effect of antioxidants and the cell biochemical changes from cytokine derived cancer cells. See Publications#5 & 6 below. These preliminary works provide feasibility for next five-year multi-state projects which emphasizes one area with the use of artificial intelligence technology for agriculture and food related research. All above accomplishments have been reported in the annual meeting of this NC 1194 multi-state project. Peer reviewed journal publications (6): 1) Hoda Ilkhani, Han Zhang, Anhong Zhou, "A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptosporidium DNA biosensor", Sensors & Actuators: B. Chemical, 2019, 282: 675-683 2) E.S. McLamore, E. Alocilja, C. Gomes, S. Gunasekaran, D. Jenkins, Y. Li, Y. Mao, S.R. Nugen, J.I. Reyes-De-Corcuera, P. Takhistov, O. Tsyusko, J.P. Cochran, T.-R. Tzeng, J.-Y. Yoon, C. Yu, A. Zhou, "A FEAST of Biosensors: Food, Environment, Agriculture, Science, Technology (FEAST) for Biosensing in North America", Biosensors and Bioelectronics, 2020, in revision. (this is a joint paper from the researchers from 15 land-grand universities supported by this multi-state project). 3) H. Zhang, A.C. Silva, W. Zhang, H. Rutigliano, A. Zhou, "Raman Spectroscopy characterization extracellular vesicles from bovine placenta and peripheral blood mononuclear cells", PLoS ONE, 2020, 15(7): e0235214. (highlighted on the journal homepage, July 10th, 2020) 4) H. Zhang, Z. Chen, J. Dai, W. Zhang, Y. Jiang, A. Zhou, "a low-cost mobile platform for whole blood glucose monitoring using colorimetric method", Microchemical Journal, 2021, 162, 105814. 5) W. Zhang, J.S. Rhodes, A. Garg, J. Takemoto, X. Qi, S. Harihar, K. R. Moon,A. Zhou, "Label-free discrimination and quantitative analysis of oxidative stress induced cytotoxicity and potential protection of antioxidants using Raman micro-spectroscopy and machine learning algorithms", Analytica Chimica Acta, 2020, 1128: 221-230. 6) W. Zhang, I. Karagiannidis, E.D.S Van Vliet, R. Yao, E. Beswick, A. Zhou, " Effects of granulocyte colony-stimulating factor on colon and breast cancer cells investigated by machine learning based non-invasive Raman spectroscopy", Analyst, 2021, 146, 6124-6131 (featured cover page). Conference presentations (8): 1) H. Ilkhani, H. Zhang, A. Zhou, "A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptosporidium DNA biosensor", 2018 Annual Institute of Biological Engineering Meeting, Norfolk, Virginia, April 5 - 7, 2018. (poster) 2) H. Zhang, A. Zhou, "A smartphone based optical platform for whole blood glucose colorimetric assay", 2019 Annual Institute of Biological Engineering Meeting, St. Louis, Missouri, April 4 - 7, 2019. (poster) 3) W. Zhang, A. Garg, H. Zhang, J. Takemoto, A. Zhou, "Cytoprotective Effect of Antioxidant on Diesel Exhaust Particles-Induced Lung Cancer Cells Damage Using Raman Spectroscopy ", 2019 Microscopy & Microanalysis (M&M 2019), August 4-8, Portland, Oregon. (poster) 4) A. Zhou, L. Xiao, Q. Li, A.K. Parchur, "Multi-functionalized nanoparticles for in vitro imaging cancer biomarker and NIR photothermal therapy of selectively targeted cancer cells using Surface-enhanced Raman spectroscopy (SERS)", 2019 Cancer Research & Therapy Conference, Oct 14-16, 2019, Orlando, Florida (podium, invited) 5) W. Zhang, R. Yao, E.D.S.V Vliet, J. Karagiannidis, E. Beswick, A. Zhou, "Effects of granulocyte colony-stimulating factor on colon and breast cancer cells investigated by machine learning based non-invasive Raman spectroscopy", A Nature conference - Transdisciplinary Cancer Interception: Leveraging Biology to Improve Prevention and Detection, organized by Huntsman Cancer Institute at the University of Utah and Nature Reviews Cancer, Huntsman Cancer Institute, Salt Lake City, Utah, March 9-11, 2020 (Poster) 6) Jacob Keim, Wei Zhang, Ying Liu, Heloisa Rutigliano, Anhong Zhou, Irina Polejaeva, "Analyzing metabolomic profile of bovine IVF and SCNT embryos through Raman spectroscopy", 46th Annual Conference of International Embryo Technology Society (IETS), January 16-19th, 2020, New York City. 7) A.C. Silva, K.P. Morgado, C.J. Davies, I.A. Polejaeva, A. Zhou, H.M. Rutigliano, "Effect of bovine trophoblast cell derived extracellular vesicles on gene expression profiles of immune cells", 53rd Society for the Study of Reproduction Annual Meeting, July 9-12, 2020, Ottawa, Canada. 8) J. Keim, W. Zhang,Y. Liu, H. Rutigliano, A. Zhou,I.A. Polejaeva, "Analyzing metabolomic profile of bovine IVF and SCNT embryos through Raman spectroscopy", International Embryo Technology Society (IETS), January 16-19, 2020, New York City.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Presentations
Zhang, H., Zhou, A., 2018 Utah Bioengineering conference, "Rapid prototyping of paper-based micro-device using wax and material printer for low-cost portable glucose assay," Salt Lake City, UT. (December 8, 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Presentations
Zhang, W., Zhou, A., 2018 Utah Bioengineering conference, "Real time monitoring fatty acid receptors on living cell using Surface-enhanced Raman spectroscopy," Salt Lake City, UT. (December 8, 2017)
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Progress 10/01/19 to 09/30/20
Outputs
Target Audience:Environmental health department employees, dairy animal care workers, water quality professionals, drinking water treatment plant and facilities operators. Changes/Problems:None What opportunities for training and professional development has the project provided?Graduate students Han Zhang and Wei Zhang from Dr. Zhou's research laboratory in Department of Biological Engineering and graduate student Ana Silva from Dr. Heloisa Rutigliano in the Department of Animal Diary and Veterinary Science joined this collaborative project. Both professors provided professional guides for these graduate students who gained the research experience from this project. In addition, we collaborated with Prof. Irina Polejaeva and her PhD student Jacob Keim from ADVS Dept on the use of Raman spectroscopy to characterize the maturation medium produced by different animal cell samples. We provided letters to strongly support Jacob Keim's USDA AFRI Graduate Fellowship application (Aug 2020). How have the results been disseminated to communities of interest?In this past year, we had 3 peer reviewed papers published, 1 manuscript is in revision, and 2 accepted conference presentations (conferences were cancelled due to the pandemic). These research findings were presented at our multi-state annual meeting (this year we did through Zoom online meeting). E.S. McLamore*, E. Alocilja, C. Gomes, S. Gunasekaran, D. Jenkins, Y. Li, Y. Mao, S.R. Nugen, J.I. Reyes-De-Corcuera, P. Takhistov, O. Tsyusko, J.P. Cochran, T.-R. Tzeng, J.-Y. Yoon, C. Yu, A. Zhou, "A FEAST of Biosensors: Food, Environment, Agriculture, Science, Technology (FEAST) for Biosensing in North America", Biosensors and Bioelectronics, 2020, in revision. (this is a joint paper from the researchers from 15 land-grand universities supported by this multi-state project) H. Zhang, A.C. Silva, W. Zhang, H. Rutigliano, A. Zhou, "Raman Spectroscopy characterization extracellular vesicles from bovine placenta and peripheral blood mononuclear cells", PLoS ONE, 2020, 15(7): e0235214. (highlighted on the journal homepage, July 10th, 2020) https://journals.plos.org/plosone/ H. Zhang, Z. Chen, J. Dai, W. Zhang, Y. Jiang, A. Zhou, "a low-cost mobile platform for whole blood glucose monitoring using colorimetric method", Microchemical Journal, 2020, Dec 2, available online. https://doi.org/10.1016/j.microc.2020.105814 W. Zhang, J.S. Rhodes, A. Garg, J. Takemoto, X. Qi, S. Harihar, K. R. Moon,A. Zhou, "Label-free discrimination and quantitative analysis of oxidative stress induced cytotoxicity and potential protection of antioxidants using Raman micro-spectroscopy and machine learning algorithms", Analytica Chimica Acta, 2020, 1128: 221-230. Two accepted conference presentations: A.C. Silva, K.P. Morgado, C.J. Davies, I.A. Polejaeva, A. Zhou, H.M. Rutigliano, "Effect of bovine trophoblast cell derived extracellular vesicles on gene expression profiles of immune cells", 53rd Society for the Study of Reproduction Annual Meeting, July 9-12, 2020, Ottawa, Canada. J. Keim, W. Zhang,Y. Liu, H. Rutigliano, A. Zhou,I.A. Polejaeva, "Analyzing metabolomic profile of bovine IVF and SCNT embryos through Raman spectroscopy", International Embryo Technology Society (IETS), January 16-19, 2020, New York City. What do you plan to do during the next reporting period to accomplish the goals?This is the last year of this multi-state project (2016-2020). In summer 2020, the researchers from more than 10 land grant universities participating this multi-state project "NC1194: Nanotechnology and Biosensors" had a group Zoom meeting and discussed the renewal proposal that outlined the new project objectives for next five-year project. I have submitted the appendix E from USU station for this renewal project.
Impacts
What was accomplished under these goals?
This is the last year of this five-year multi-state project. The main technical progress in this past reporting period covers the following objectives: Objective#1 Develop new technologies for characterizing fundamental nanoscale processes. We have been working on the characterization of extracellular vesicles (EVs) or exosomes with typical size of 40-120 nm. Placenta-derived extracellular vesicles (EVs) are involved in communication between the placenta and maternal immune cells possibly leading to a modulation of maternal T-cell signaling components. The ability to identify EVs in maternal blood may lead to the development of diagnostic and treatment tools for pregnancy complications. It is necessary to develop a label-free, non-invasive Raman spectroscopy technique to characterize and differentiate EVs from bovine placenta (trophoblast) and peripheral blood mononuclear cells (PBMC). Objective#2 Construct and characterize self-assembled nanostructures. To address this objective, we continued our collaboration with Prof. Heloisa Rutigliano's group in using non-invasive Raman spectroscopy technique to characterize the nanoscale EVs isolated from different animal cell samples. Our Raman results indicated that Raman peaks at 728 cm-1 (collagen) and 1573 cm-1(protein) were observed only in PBMC-derived EVs, while the peaks 702 cm-1 (cholesterol) and 1553 cm-1 (amide) appeared only in trophoblast-derived EVs. Principal component analysis and linear discriminant analysis were applied to discriminate of the Raman spectral fingerprints for both types of EVs from different animals. Moreover, the PBMC-derived EVs from different animals were indistinguishable, while the trophoblast-derived EVs from three placental samples of different gestational ages showed separate clusters. This study reports for the first time the Raman characteristic peaks for identification of PBMC and trophoblast-derived EVs. The development of this method also provides a potential tool for further studies investigating the causes and potential treatments for pregnancy complications.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Zhang, H., Silva, A. C. X., Zhang, W., Rutigliano, H., & Zhou, A. (2020, July 07). Raman Spectroscopy characterization extracellular vesicles from bovine placenta and peripheral blood mononuclear cells. PLOS ONE.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Zhang, W., Rhodes, J., Moon, K., Knudsen, B., Nokolova, L., & Zhou, A. (2020, October). Imaging of PD-L1 in single cancer cells by SERS-based hyperspectral analysis. Biomedical Optics Express, 11(11), 6197-6210.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Zhang, W., Rhodes, J., Garg, A., Takemoto, J. Y., Qi, X., Harihar, S., Chang, C. W. T., Moon, K., & Zhou, A. (2020, September). Label-free Discrimination and Quantitative Analysis of Oxidative Stress Induced Cytotoxicity and Potential Protection of Antioxidants Using Raman Spectroscopy and Machine Learning. Analytica Chimica Acta, 1128, 221-230.
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Progress 10/01/18 to 09/30/19
Outputs
Target Audience:Environmental health department, water quality, drinking water treatment plant and facilities. Changes/Problems:In the past year, we experienced unstable sensor responses after gold nanoparticles were introduced to sensor surface. In this coming year, we will find the solution to solve this technical challenge. What opportunities for training and professional development has the project provided?One Biological Engineering undergraduate student Kade Derrick involved partially the project under the supervision of the graduate student Wei Zhang from PI Dr. Zhou's research laboratory. Different deposition methods of gold nanoparticles were tried. How have the results been disseminated to communities of interest?The research findings from this project were presented to the undergraduate course BENG 1800 (Introduction to Undergrad Research) with 100+ freshmen engineering students at USU. The graduate student also presented their research results at the 2019 annual conference of the Institute of Biological Engineering. Jacob Keim, Wei Zhang, Ying Liu, Heloisa Rutigliano, Anhong Zhou, Irina Polejaeva, "Analyzing metabolomic profile of bovine IVF and SCNT embryos through Raman spectroscopy", 46th Annual Conference of International Embryo Technology Society (IETS), January 16-19th, 2020, New York City. What do you plan to do during the next reporting period to accomplish the goals?In next year, we will fix the influence of the introduction of gold nanoparticles to the unstable sensor response, by optimizing the experiment conditions of nanoparticle deposition and DNA conjugation and hybridization. Our goal is to achieve the desired sensor sensitivity and repeatability.
Impacts
What was accomplished under these goals?
In the previous year, we successfully constructed our prototype of DNA sensor and completed the evaluation of the sensor performance. In this past reporting period, the main efforts were devoted to the following two objectives: Objective#1 Develop new technologies for characterizing fundamental nanoscale processes. DNA immobilized on sensor surface (via biolink agents) is considered to be in nanoscale. The appropriate approaches that facilitate the efficiency of DNA-mediated electron transfer characteristics on modified sensor surface are critical to improve the overall performance of signed DNA biosensors. In this past year, a number of approaches, including the use of gold nanoparticles with various sizes, optimization of sensor chamber dimension, were explored for this purpose. Objective#2 Construct and characterize self-assembled nanostructures. The gold nanoparticles with 20 nm and 50 nm were deposited on sensor surface to increase the sensitivity of our DNA sensors. We did observe the difference in electrochemical responses of DNA-DNA hybridization when applied 25 nm and 50 nm nanoparticles on the sensor surface prior to DNA immobilization and hybridization. The overall performance such as sensitivity, selectivity, detection limit, and linear range was evaluated accordingly in each of steps of self-assembled gold nanoparticles with the subsequent immoblizaiton of probe DNA, target DNA, and hybridization of probe--target sequences binding. We are still optimizing nanoparticle deposition conditions (e.g., time, concentration, pH, buffer, etc.) in order to obtain better repeatability of sensor responses. Another technical challenge was the introduction of a new layer of gold nanoparticles would enhance the electrochemical signal, but meanwhile, it also influenced the subsequent DNA immobilization and hybridization conditions, thus leading to unstable sensor responses. We need to explore other methods to balance between the enhanced sensor signal and instability of signals. The protocol of previously defined DNA conjugations would be revisited, and should be adjusted as needed in this coming year. In this past year, we continued the collaborations with two faculty members, Prof. Heloisa Rutigliano (exosomes derived from bovine placenta and PMBC) and Prof. Irina Polejaeva (metabolite profiling of bovine IVF and SCNT embryos) from the Department of Animal, Dairy & Veterinary Sciences (ADVS) by using our non-invasive Raman spectroscopy technique.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Ilkhani, H., Zhang, H., & Zhou, A. (2019, January). A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptospordium DNA biosensor. Sensors and Actuators B: Chemical, 282.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Zhang, H., Smith, E., Zhang, W., & Zhou, A. (2019, June). Inkject printed microfluidic paper-based analytical devices (�PAD) for glucose colorimetric detection in artificial urine. Biomedical Microdevices.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Zhang, W., Xiao, H., & Zhou, A. (2019, March). Smartphone colorimetric detection of calcium and magnesium in water samples using a flow injection system. Microchemical Journal.
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Progress 10/01/17 to 09/30/18
Outputs
Target Audience:Environmental health department, water quality, drinking water treatment plant and facilities. Changes/Problems:None What opportunities for training and professional development has the project provided?Two undergraduate students from Mechanical Engineering (Reem Ghabayen, Kamila Khamidova) were recruited in early fall semester 2018 to join this project. Both student contributed the AutoCAD design of prototype sensor device. Graduate students Han Zhang and Wei Zhang from PI Dr. Zhou's research laboratory have provided training to these two undergraduates. How have the results been disseminated to communities of interest?The research findings from this project were presented to the undergraduate course BENG 1800 (Introduction to Undergrad Research) with 90+ freshmen engineering students at USU. The graduate students also presented their research findings at the 2018 annual conference of the Institute of Biological Engineering. In this past year, we published one peer reviewed paper: Hoda Ilkhani, Han Zhang, Anhong Zhou, "A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptosporidium DNA biosensor", Sensors & Actuators: B. Chemical, 2019, 282: 675-683. What do you plan to do during the next reporting period to accomplish the goals?In next year, we will complete the assessment of our fabricated DNA biosensor for detection of a synthetic human genotype of Cryptosporidium. We have achieved a lower detection limit (~nM) of target oligo sequence. Based on the assessment results, we may consider alternative approaches to improve the sensitivity, e.g., the use of nanoparticles. We will also continue our non-invasive measurement of exosome nanoparticles in conjunction with the morphology characterization by instruments SEM and DLS.
Impacts
What was accomplished under these goals?
The main technical progress in this past reporting period covers the following objectives: Objective#1 Develop new technologies for characterizing fundamental nanoscale processes. Understanding of the DNA-mediated electron transfer characteristics on sensor surface plays essential roles in design of highly sensitive DNA biosensors. We have applied a variety of electrochemical techniques, including cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy to quantitatively assess the electron transfer behaviors of each step of construction of DNA biosensors, such as probe DNA immobilization, probe-target hybridized surface, capture probe DNA hybridization, and electroactive ALP-linked enzyme. We obtained the optimal sensor design based on these assessment results. Objective#2 Construct and characterize self-assembled nanostructures. Starting with the first step of immobilizing probe DNA (thiolated oligonucleotides), the construction of DNA biosensor includes multiple self-assembled steps to finally form highly specific DNA structure on sensor surface for recognizing the target DNA sequence. The experimental conditions of each self-assembled step are optimized. The overall performance such as sensitivity, selectivity, detection limit, and linear range have been evaluated for hybridization with complementary, single-base, three-base, and five-base mismatched target sequences. To address this objective, we also initiated a collaboration with Prof. Heloisa Rutigliano's group in using non-invasive Raman spectroscopy technique to characterize the nanoscale extracellular vesicles (EVs) isolated from different animal cell samples. Objective#3 Develop devices and systems incorporating microfabrication and nanotechnology. A prototype microfluidic device was fabricated using photolithograph techniques, and tested in detection of various target sequences (with different base-mismatch) to achieve satisfactory results. Our DNA biosensor for the first time realized the separation of the probe/target DNA immobilized sensor part (disposable) from the detection of electron-transfer sensor part (reusable), making it well suitable for multiplex detection of other species-specific target DNA with similar sequences (to test specificity). In this past year, we have been successfully testing our DNA sensor with the target sequence specific to human genotype of Cryptosporidium. Our results indicate a detection of limit of target sequence with six times lower sensitivity, compared to the literature reported method.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Presentations
Ilkhani, H., Zhang, H., Zhou, A., 2018 Annual Institute of Biological Engineering Meeting, "A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptosporidium DNA biosensor," Norfolk, Virginia. (April 5, 2018 - April 7, 2018)
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Progress 05/08/17 to 09/30/17
Outputs
Target Audience:Environmental health department, drinking water treatment plant and facilities. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?One undergraduate (Ethan Smith) was recruited in the summer 2017 to join this project. Graduate students Han Zhang and Wei Zhang from PI Dr. Zhou's research laboratory has provided training to this undergraduate with the sensor device fabrication and tests. How have the results been disseminated to communities of interest?The research finding from this project was presented to the undergraduate course BENG 1800 (Introduction to Undergrad Research) with 100+ freshmen engineering students at USU. The graduate students also presented their posted in 2017 Utah Bioengineering conference in December 2017. The research results from this reporting period will be included in a manuscript in preparation. What do you plan to do during the next reporting period to accomplish the goals?In next year, we will continue the optimization of sensor device design and fabrication, and assessment of the sensor device performance in detection of synthetic human genotype of Cryptosporidium in terms of sensitivity, selectivity, and detection limit of DNA. We expect to obtain a detection limit of ~nM or lower concentration of target oligo sequence. In order to improve these performance factors, we explore also optimize the device fabrication protocols, as well as electrochemical signal amplification using alternative approaches, for example, the use of nanoparticles. We will also start contacting the Utah Department of Health and/or Utah Department of Environmental Quality to learn more about the species and genotypes of Cryptosporidium that impact significantly in the human health and animal care in Utah, and also learn the current detection methods used in these state laboratories to diagnose and identify these species or genotypes.
Impacts
What was accomplished under these goals?
The main technical progress in this past reporting period was associated with the following objectives: Objective #1: Develop new technologies for characterizing fundamental nanoscale processes. To characterize the DNA-modified surface, we conducted assessment of the DNA-mediated electron transfer related electrochemical behaviors using a variety of electrochemical techniques, such as cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy for each DNA sensor. These steps include the hairpin shape probe DNA immobilization, target sequence hybridization on probe DNA coated sensor surface, followed by hybridization with capture probe, streptavidin alkaline phosphate enzyme linking on DNA sensor surface, finally, the electrochemical enzymatic reaction measurement. Objective #2: Construct and characterize self-assembled nanostructures. The optimization of experimental conditions of each step of above mentioned DNA immobilization and hybridization, and electroactive enzyme conjugation were started and more investigational experiments are still ongoing. Objective #3: Develop devices and systems incorporating microfabrication and nanotechnology. A microfluidic device hosted the patterned three-electrode layout including the working electrode for the measurement of the electrochemical reactions with the gold dot electrode has been prototyped.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Li, Q., Tang, M., Zhou, A. (2017). In vitro detection of diesel exhaust particles induced human lung carcinoma epithelial cells damage and the effect of resveratrol. Journal of Applied Toxicology, 37(6), 747-757
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Zhang, H., Zhou, A., 2017 Regional meeting of Institute of Biological Engineering, "Inkjet Printed �Pad for Urine Glucose Colorimetric Testing," Logan, UT. (November 11, 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Zhang, W., Zhou, A., Liu, Y., Gilbertson, T. A., 2017 Regional meeting of Institute of Biological Engineering, "Real time monitoring fatty acid responsive receptors on living cells using Surface-enhanced Raman spectroscopy (SERS)," Logan, UT. (November 11, 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Zhang, H., Zhou, A., 2017 Annual Institute of Biological Engineering Meeting, "Rapid prototyping of paper-based micro-device using wax and material printer for low-cost portable glucose assay," Salt Lake City, UT. (March 30, 2017 - April 1, 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Zhang, W., Zhou, A., 2017 Annual Institute of Biological Engineering Meeting, "Smartphone-based colorimetric detection of calcium and magnesium continuous detection in water sample," Salt Lake City, UT. (March 30, 2017 - April 1, 2017)
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