Progress 05/01/17 to 04/30/21
Outputs
Target Audience:Target audiences: Diverse stakeholders including students of agriculture and life sciences, scientists from academia and industry in the US and around the world, livestock producers in the US and globe, foundations, and government organizations. The audiences also include individuals from diverse gender including women and minorities, ethnic groups, age (from students to retired people), geographic locations (from the US to Southeast Asia), ways of thinking, knowledge and abilities, social and economic, and belief systems. Efforts: Extensive efforts have been put it in the following: Formal classroom and laboratory instructions on animal science, active participation in practicum and experiential learning, outreach, presentations at symposia at Mississippi State University, US, Indonesia, Brazil, Turkey and Czech Republic. Changes/Problems:The COVID-19 pandemic has made significant negative impact on the research; the shelter in place and the restrictions slowed down the research. The pandemic also slowed down the communications among the collaborators and publishing organizations. What opportunities for training and professional development has the project provided?Undergraduate, graduate and postdoctoral students have been empowered with advanced knowledge in animal agriculture, production of cattle for food security, bull fertility, modern biology, scientific thinking and experimental design, generation and analyses of data and interpretation of the results. The students developed technical skills in animal andrology, metabolomics, data analyses, scientific writing, and publications and presentations. How have the results been disseminated to communities of interest?The new knowledge generated has been disseminated through presentations and publications (the list provided previously). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We have identified metabolites in seminal plasma and sperm from Holstein bulls with different fertility using GC-MS and then analyzed the data to identify patterns and relationships among the metabolomes.?
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Evans HC, Dinh TTN, Hardcastle ML, Gilmore AA, Ugur MR, Hitit M, Jousan FD, Nicodemus MC, Memili E. (2021) Advancing semen evaluation using lipidomics. Frontiers in Veterinary Science, 8:601794. doi: 10.3389/fvets.2021.601794.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Gilmore AA, Hitit M, Ugur MR, Dinh TTN, Wei Tan, Jousan DF, Nicodemus MC, Topper E, Kaya A, Memili E. (2021) Functional variables of bull sperm associated with cryotolerance. Kafkas Univ Vet Fak Derg 27(3):371-379.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
�zbek M, Hitit M, Kaya A, Jousan FD, Memili E. (2021) Molecular signatures of bull sperm for accurate evaluation of semen quality. Frontiers in Veterinary Science, 8:610888. doi: 10.3389/fvets.2021.610888.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Viana AGA, Ribeiro IM, Memili E, Moura AAA, Machado-Neves M. (2021) Effects of seminal plasma proteins on bull fertility: An integrative review. Reproduction, 161(4):459-475. doi: 10.1530/REP-20-0392.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Uncovering Sperm Biomarkers of Bull Fertility Using Multiomics Approaches. (2020) International Plant and Animal Genome (PAG) Conference. San Diego, CA. January 12, 2020.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
Biomolecular Markers and Mechanisms for Sperm Male Fertility and Stem Cell Regenerative Medicine. (2020) Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University. Atlanta, GA. January 29, 2020
- Type:
Conference Papers and Presentations
Status:
Awaiting Publication
Year Published:
2022
Citation:
Memili, E. Sperm Signatures of Bull Fertility and Sperm Freezability.
This is an invited plenary speaker at the Association for Applied Andrology's biennial conference in Bologna, Italy in June 2022. The article will be published in the peer-reviewed journal of Animal Reproduction Science.
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Progress 05/01/19 to 04/30/20
Outputs
Target Audience:Diverse groups of audiences have been targeted and reached. For example, through the conduct of research, graduate students were empowered with technical and soft skills and these students have resented their research to other students and faculty members. Throughpeer-reviewed publications, students, scientists, animal producers, educators, and the public have been reached out. The PI, Dr. Memili was an invited plenary speaker at the international conference of the Association for Applied Animal Andrology (AAAA) scheduled to take place in Bolognia, Italy in the summer of 2020. Although the conference was canceled due to COVID-19, the proceedings including the PI's article was published and made available to the global community through the Journal of Animal Reproduction Science. Some of the research results were also presented at other conferences including the Plant and Animal Genome Conference. Changes/Problems:Initially, we had a significant delay of several months because of the delays in receiving the sperm and seminal plasma samples from our industry partners, Alta Genetics, Inc. When the samples were received, we proceeded with the funded project and generated large amounts of data and results. We have requested a no-cost extension which has helped the research team including the students to have time to continue the research. However, since early 2020, due to COVID-19, our research progress has slowed down and we could not complete the remaining experiments to validate our results. As such, a further extension is needed to complete the validation experiments and analyze the data. What opportunities for training and professional development has the project provided?Quality training programs have been provided to undergraduate, graduate, and postdoctoral students both in technical laboratory settings and in quality mentoring which helped students develop sound soft skills including scientific communication skills. How have the results been disseminated to communities of interest?The results of the research were disseminated through peer-reviewed publications and conference presentations. The list of publications is also provided through the PI's web page at Mississippi State University. What do you plan to do during the next reporting period to accomplish the goals?We plan to validate our research findings to complete the projects in the funded research.
Impacts
What was accomplished under these goals?
Peer-reviewed publications: Through the research, we have produced new knowledge and uncovered metabolomic profiles of sperm and seminal plasma from bulls with different fertility phenotypes. Fertility markers: We have determined biomolecular markers in sperm and seminal plasma that can be used to evaluate semen quality and predict bull fertility. Empowered graduate students: Students developed both technical and soft skills. New data and results generated advance both fundamental and applied science with a positive impact on science and society.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Lipidomic markers of sperm cryotolerance in cattle.
Evans HC, Dinh TTN, Ugur MR, Hitit M, Sajeev D, Kaya A, Topper E, Nicodemus MC, Smith GD, Memili E.
Sci Rep. 2020 Nov 19;10(1):20192. doi: 10.1038/s41598-020-77089-9.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Metabolomes of sperm and seminal plasma associated with bull fertility.
Memili E, Moura AA, Kaya A.
Anim Reprod Sci. 2020 Apr 1:106355. doi: 10.1016/j.anireprosci.2020.106355. Online ahead of print.
PMID: 32273206
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Uncovering sperm metabolome to discover biomarkers for bull fertility.
Menezes EB, Velho ALC, Santos F, Dinh T, Kaya A, Topper E, Moura AA, Memili E.
BMC Genomics. 2019 Sep 18;20(1):714. doi: 10.1186/s12864-019-6074-6.
PMID: 31533629
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Hitit M, Kaya A, Memili E. (2020) Sperm Functional Genome and Epigenome Associated with Freezability. Presented at the 2nd FAANG-Europe Workshop on Functional Annotation of Animal Genomes. Prague, Czech Republic. February11-13, 2020.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Ugur MR, Kutchy NA, Menezes EB, Ul-Husna A, Hitit M, Uzun A, Moura A, Dinh T, Kaya A, Memili E. (2019). Sperm functional genome and epigenome regulating bull fertility and sperm freezability. Gordon Research Conference on Fertilization and Activation of Development. Holderness, NH. July 28-August 2, 2019.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Kutchy NA, Menezes E, Velho A, Moura A, Kaya A, Dinh T, Memili E. (2019) Sperm Functional Genome Associated with Bull Fertility. International Plant and animal Genome Conference. San Diego, CA. January 12-16, 2019.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Menezes E, Velho A, Santos F, Dinh T, Kaya A, Topper E, Didion B, Moura A, Memili E. (2019) Sperm Metabolomic Landscape Associated with Bull Fertility. Annual Conference of the International Embryo Technology Society. New Orleans, LA. January 20-23, 2019.
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Progress 05/01/18 to 04/30/19
Outputs
Target Audience:The diverse audience has been the direct beneficiary of the current project. The audience includes students, researcher and livestock producers from Mississippi, the United States, and the world. The audience includes women and underrepresented minorities. Both the local and national agricultural communities have been informed about the research results through conference presentations and publications. The science-based solutions for enhancing cattle reproduction and production efficiency has paramount importance both for economic reasons for enhancing the quality of life. Changes/Problems:During the course of research thus far, we have encountered a major delay in the attainment of sperm and seminal plasma samples from bulls with reliable different fertility phenotypes. Although securing the samples caused us a delay of more than eight months, we were able to pursue the original research and obtain quality preliminary results some of which have already been published or submitted for publication in high caliber journals. As such, we have requested a no-cost extension which is vital for completions of the project, so that we will be able to fully accomplish the goals. What opportunities for training and professional development has the project provided?Diverse students including two postdoctoral research associates (one female, one male), four graduate students (two females and two males) and a female undergraduate student have developed essential skills in contemporary animal science and in the cutting-edge science of metabolomics. In addition, the students have attained vital soft skills including scientific thinking, experimental design, data analyses and interpretation of results, presentations of scientific results at national and international conferences, and research manuscript and proposal writing. How have the results been disseminated to communities of interest?The early findings on seminal plasma metabolomes were published as an article in the prestigious peer-reviewed scientific journal, PLoS One, and findings on sperm metabolomics in a manuscript have been accepted publication in the peer-reviewed journal BMC Genomics. We have also submitted our findings on lipidome of sperm and amino acids of seminal plasma to Scientific Reports and Frontiers in Cell and Developmental Biology, respectively. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, we have specific and attainable research objectives: Confirm the metabolomic markers identified in our preliminary studies in sperm and seminal plasma samples. Ascertain patterns and relationships among the fertility marker metabolomes to develop extenders to increase post-thaw viability of bull sperm.
Impacts
What was accomplished under these goals?
The impact is that research has produced potential biomarkers that are powerful fertility markers to evaluate semen quality and predict bull fertility. The issue is that despite thousands of dollars economic impacts, there exists no reliable set of fertility markers to determine semen quality and predict male fertility. Despite producing ample amounts of sperm with normal morphology and motility, some bulls are afflicted with subpar fertility. Current conventional tests are not able to discern the sperm attributes associated with low fertility. There is a gap in the knowledgebase in that molecular mechanisms regulating the low fertility. The objective of this research is to test the hypothesis that sperm and seminal plasma metabolomes are associated with bull fertility using metabolomics and computational biology approaches. Our preliminary findings have revealed the list of differentially expressed metabolites in sperm and seminal plasma of bulls with different field fertility phenotypes.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Ugur MR, Alsharawy A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. Advances in cryopreservation of bull sperm. Frontiers in Veterinary Science.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Ugur MR, Kutchy NA, Menezes EB, Ul-Husna A, Hitit M, Uzun A, Moura A, Dinh T, Kaya A, Memili E. (2019). Sperm functional genome and epigenome regulating bull fertility and sperm freezability. Gordon Research Conference on Fertilization and Activation of Development. Holderness, NH. July 28-August 2, 2019.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2029
Citation:
Kutchy NA, Menezes E, Velho A, Moura A, Kaya A, Dinh T, Memili E. (2019) Sperm Functional Genome Associated with Bull Fertility. International Plant and animal Genome Conference. San Diego, CA. January 2019.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2019
Citation:
Evans HC, Dinh T, Ugur MR, Hitit M, Sajeev D, Kaya A, Topper E, Nicodemus MC, Smith GD, Memili E. Lipidomic Markers of Sperm Cryotolerance. Submitted, under review; Scientific Reports.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2019
Citation:
Ugur MR, Dinh T, Hitit M, Kaya A, Topper E, Didion B, Memili E. Amino Acids of Seminal Plasma Associated with Freezability of Bull Sperm. Submitted, under review; Frontiers in Cell and Developmental Biology.
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2019
Citation:
Menezes E, Velho A, Santos F, Dinh T, Kaya A, Topper E, Moura A, Memili E. Uncovering Sperm Metabolome to Discover Biomarkers for Bull Fertility. In press. BMC Genomics.
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Progress 05/01/17 to 04/30/18
Outputs
Target Audience:Diverse audience has been the direct beneficiary of the current project. The audience include students, researcher and livestock producers from Mississippi, the United States, and the world. The audience include women and underrepresented minorities. Both the local and national agricultural communities have been informed about the research results through conference presentations and publications. The science-based solutions for enhancing cattle reproduction and production efficiency has a paramount importance both for economic reasons for enhancing the quality of life. Changes/Problems:During the course of research thus far, we have encountered a major delay in the attainment of sperm and seminal plasma samples from bulls with reliable different fertility phenotypes. Although securing the samples caused us a delay of more than eight months, we were able to pursue the original research and obtain quality preliminary results some of which have already been published or submitted for publication in high caliber journals. As such, we will be requesting a no-cost extension, vital for thecompletion of the project, so that we will be able to fully accomplish the goals. What opportunities for training and professional development has the project provided?The project has provided valuable opportunities for diverse students. The students included undergraduate, graduate, postdoctoral as well as international visiting scholars. The students have developed essential skills in molecular biology as well as in scientific communication. How have the results been disseminated to communities of interest?The early findings on seminal plasma metabolomes were published as an article in the prestigious peer-reviewed scientific journal, PLoS One, and the preliminary findings on sperm metabolomics in a manuscript have been submitted to the peer-reviewed journal BMC Genomics. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, we have specific and attainable research objectives: 1. To validate the potential fertility markers of metabolites identified in our preliminary studies in sperm and seminal plasma. 2. Ascertain quantitatively the metabolome markers. 3. Identify patterns and relationships among the fertility marker metabolomes to develop extenders to increase post-thaw viability of bull sperm.
Impacts
What was accomplished under these goals?
The impact is that research has produced potential biomarkers that are powerful fertility markers to evaluate semen quality and predict bull fertility. The issue is that despite thousands of dollars economic impacts, there exists no reliable set of fertility markers to determine semen quality and predict male fertility. Despite producing ample amounts of sperm with normal morphology and motility, some bulls are afflicted with subpar fertility. Current conventional tests are not able to discern the sperm attributes associated with the low fertility. There is a gap in the knowledgebase in that molecular mechanisms regulating the low fertility. The objective of this research is to test the hypothesis that sperm and seminal plasma metabolomes are associated with the bull fertility using metabolomics and computational biology approaches. Our preliminary findings have revealed list of differentially expressed metabolites in sperm and seminal plasma of bulls with different field fertility phenotypes.
Publications
- Type:
Journal Articles
Status:
Submitted
Year Published:
2018
Citation:
Menezes E, Velho A, Santos F, Dinh T, Kaya A, Topper E, Moura A, Memili E. Uncovering Sperm Metabolome to Discover Biomarkers for Bull Fertility. Submitted to BMC Genomics.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Velho A, Dihn T, Menezes E, Kaya A, Topper E, Moura A, Memili E. (2018) Metabolic markers of fertility in bull seminal plasma. PLoS ONE 13(4): e0195279. https://doi.org/10.1371/journal.pone.0195279
- Type:
Book Chapters
Status:
Published
Year Published:
2017
Citation:
Velho ALC, Oliveira R, Dinh T, Moura A, Kaya A, Memili E. (2017) Applications of metabolomics in reproductive biology. In Animal Models and Human Reproduction. Ed. Heide Schatten and George Constantinescu. John Wiley & Sons, Inc.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Moura AA, Memili E, Moemia A, Portela R, Viana AG, Velho ALC, Bezerra MJB, Vasconselos FR. (2018) Seminal Plasma Proteins and Metabolites: Effects on Sperm Function and Potential as Fertility Markers. Proceedings of the 10th International Ruminant Reproduction Symposium (IRRS 2018); Foz do Igua�u, PR, Brazil, September 16th to 20th, 2018. Animal Reproduction. v.15, (Suppl.1), p.691-702. DOI: 10.21451/1984-3143-AR2018-0029
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