Progress 05/01/17 to 04/30/22
Outputs Target Audience:The target audience for the Mobile Responsive Clinical Fish Health Database includes fish disease diagnostic laboratories around the United States and a few in foreign countries that benefit by having their fish disease diagnostic records organized digitally, making retreiving data more efficient and doing epidemiology possible. Included in target audiences involved in diagnosing fish diseases are private veterinarian groups, university research teams, and students (graduate and undergraduate) learning about fish diseases, databases, and epidemiology. There were also computer learning games (which were part of the grant to develop the Database) that reached yet another target audience: high school students, some of which are exposed to closed, recirculating aquaculture systems that, at times, have sick fish that students must deal with. Changes/Problems:As described under accomplishments, the original plan to develop an application (app) was modified (due to evolving technology that has replaced "apps" with mobile-responsive sites) to the creation of a mobile responsive clinical fish health database that can be used on a computer screen (in a larger format) or on a smaller mobile device such as a mobile phone (in a format/size that fits the mobile device) that is more adaptable for field use. Another change (also described under accomplishments) was to replace the computer game FishDetective with FishRun due to technical/administrative reasons at Oregon State University. What opportunities for training and professional development has the project provided?Undergraduates and graduate students at Kentucky State University and Oregon State University have been exposed to the Database via its beta testing. Fish pathologists and veterinarians have been exposed to the database during beta testing and have been able to view photographs and videos of fish pathogens providing experiences with pathogens that they may not have encountered first-hand previously. How have the results been disseminated to communities of interest?University researchers, private veterinarians, graduate students, and undergraduates have been given access to the mobile responsive clinical fish health database to use for their benefit and for beta testing (for the benefit of the research team working interatively to improve/"fine-tune" the database). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
The original project objective was to create a fish disease case database with a separate mobile app, but with evolving technology, the project was modified to creating a mobile responsive clinical fish health database where the database is online but also accessible by mobile devices, e.g., cell phones, that can use the database in a mobile-responsive format that "fits" the smaller screen and can be used in the field. This database was successfully created iteratively by working with a local software development company that was able to develop the database site with feedback from Kentucky State University. All KSU historical data were entered with a bulk import script. New fish disease cases are currently being entered on a weekly basis including information on body of water affected, water quality, fish feeding activity, total number of dead fish, number of fish dying per day, behavior of the fish, external and internal clinical signs of moribund fish, parasites found on or in the fish, identified bacteria isolated from fish samples and their susceptibility to various antibiotics (including zones of inhibition), previous treatments used and recommended treatments, as well as photographs and video clips to be used for disease documentation as well as for instructing anyone learning details of fish diseases (including veterinarians or fish pathologists that are new to the field as well as graduate and undergraduate students - or high school students). Old and/or new diagnostic data are able to be entered into the database via the online website or mobile devices, and key words can be searched by using the filtering function. The database can be used for epidemiology, studying things such as the frequency that bacteria are susceptible to certain antibiotics, and locations where certain diseases occur, and management practices that might be correlated with preventing disease, for example. The database, itself, is hosted by Amazon Web Services (AWS) and is affiliated with Oregon State University's database www.fishpathogens.net which has been secured for several years as a domain name. Macroscopic and microscopic images as well as videos and text descriptions have been added to the site. Other fish disease diagnostic laboratories in the U.S. have used the mobile responsive clinical fish health database (beta testing of it) for entering data and/or providing constructive feedback on the database including labs at the Mississippi State University School of Veterinary Medicine, University of Arkansas at Pine Bluff, Louisiana State University, and Auburn University. Oregon State University successfully developed two computer learning games SimFish, and FishRun that have been beta tested by graduate and undergraduate students at OSU as well as during a microbiology "bootcamp" for high school students in 2022. Beta testing is also planned as a follow-up/continuation to this grant. These games were developed by OSU undergraduate students, giving them valuable experience in software coding. In SimFish (a Tamagotchi-style electronic pet game) the player must keep their pet fish healthy over several days by monitoring tank conditions, feed level, health status and treating pathogen challenges with appropriate antibiotics/antivirals. In FishRun, (a sideways scrolling action game) the player controls a swimming fish as it avoids obstacles and pathogens - if the player's fish hits a pathogen, the player can rescue themselves to swim again by correctly answering a simple quiz that educates them about an aspect of fish health. Two games were originally planned for being created, but FishRun had to replace FishDetective due to OSU administrative policy changes.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2019
Citation:
Mitchell, K.M. and R.M. Durborow. 2019. Largemouth bass diseases. Chapter in
Largemouth Bass Aquaculture. James H. Tidwell, Shawn Coyle, and Leigh Anne Bright, editors. 5M Publishing, Benchmark House, Sheffield, UK. 274 pp.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Durborow, R.M., S. Atkinson, A. Poudel, T. McKay, and A. Sabek. 2019. Developing a clinical database, mobile application (app), and learning games for fish disease diagnostics. Poster presented at: Aquaculture Europe 2019, Berlin, Germany, October 7 10.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Durborow R.M., T.J. McKay, S. Atkinson, J. Kelso, and McKay, T.W. 2022. Mobile Responsive Clinical Fish Health Database. Presented at: World Aquaculture Society, San Diego, CA, March 2022.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
McKay, T.J., T.W. McKay, J. Kelso, A. Poudel, and R.M. Durborow. 2022. Developing a Mobile Responsive Clinical Fish Health Database. Presented at: Association of 1890 Research Directors Symposium, Atlanta, GA, April 2022.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
McKay, T.J., T.W. McKay, J. Kelso, A. Poudel, and R.M. Durborow. 2022. Developing a Mobile Responsive Clinical Fish Health Database. Presented at: World Aquaculture Society, San Diego, CA, February 2022.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2021
Citation:
McKay, T.J., T.W. McKay, J. Kelso, A. Poudel, and R.M. Durborow. 2021. Developing a Mobile Responsive Clinical Fish Health Database. Presented at: World Aquaculture Society, San Antonio, TX, August 2021.
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Progress 05/01/20 to 04/30/21
Outputs Target Audience:During Aquaculture Europe 2019 in Berlin, Germany in October, at the combined poster session and trade show arena, valuable contacts were made with key target audiences that were exhibiting at the trade show. These included a Greek i.t. company led by Vasailis Foteinos working on IMPAQT (Intelligent Management System for Integrated Multi-trophic Aquaculture); Observations Medical Outcomes Partnership (OMOP) Database via Neha Mandadi; INVE (represented by Mayleen Chang Vasquez) developing a database for their fish disease diagnostics records at their Thai facility; Rosa Merino of Hipra interested in the database for their vaccine production in Catalonia, Spain; Biomin (represented by Dr. Silvia) for their disease/nutrition database; Shane Hunter, owner of Aqua Bio Tech in Malta, working with Dr. Tomas on the iFishIENCi database; and Dr. Ariadna Sitja-Bobadilla, project director for ParaFishControl database that tracts disease occurrence among aquacultured animals in ocean net-pens. These seven organizations/target audiences expressed interest in collaborating with us upon completion of the Clinical Fish Health Database. In addition, during the week preceeding the conference, the grant PD made visits to target audiences in Hannover and Munich, Germany; at Hannover University School of Veterinary Medicine and at Technical University of Munich. Collaborations were discussed with Dr. Deiter Steinhagen, of Hannover University and Dr. Ralph Kuehn at TUM (Munich, Germany). Other target audiences include fish disease diagnostic laboratories in the U.S. including Louisiana State University School of Veterinary Medicine (Dr. John Hawke), Mississippi State University College of Veterinary Medicine (Dr. Larry Hanson), and the Alabama Fish Farming Center in Marion, Alabama (a division of Auburn University).Private veterinarian Dr. Craig Blair who has a veterinary medicine practice in Lexington, Kentucky, has also agreed to collaborate on data collection using the Database. And, Kentucky State University and Oregon State University students will be involved in beta testing of the Database, serving as an important target audience. Changes/Problems:The challenge of completing the Database during the pandemic will be overcome during the requested second no-cost extension currently under consideration by USDA. What opportunities for training and professional development has the project provided?Graduate and undergraduate students enrolled in Fish Diseases (AQU 511 and AQU 411) and Fish Diseases Laboroatory (AQU 510 & AQU 410) taught during fall 2020 semester were able to beta test the Database, entering test cases in order for them to experience the use of a clinical database and to get feedback from them on how the Database functioned. How have the results been disseminated to communities of interest?At this point in the Database development, the only dissemination was to the Fish Diseases Laboratory students during their beta testing experience. Dissemination to other groups will occur when further progress is made on the Database. What do you plan to do during the next reporting period to accomplish the goals?Kentucky State University and Oregon State University will continue to work with Apax in the development of the Clinical Fish Health Database. Feedback from students, other fish health diagnostic laboratories, and private veterinarians will be used during the beta testing stage to improve the functioning efficiency of the Database. The Database will be more fully integrated with FishPathogens.net, the Oregon State University hosting web site. The computer learning games will also be completed during the upcoming year, which will be the second no-cost extension requested from USDA.
Impacts What was accomplished under these goals?
Progress has continued this year in developing the Clinical Fish Health Database with Apax, Inc., information technology company. Graduate student Tyler McKay continued his work with funding from this grant. He has been involved in diagnosing fish diseases during this year including necropsy procedures to determine cause of death as well as biochemical identification of bacteria causing disease and antibiotic susceptibility testing to determine the best treatment of medicated feed. Fish parasites and water quality problems associated with the disease cases were also identified; proper treatments for each were recommended to clientele using our fish disease diagnostic laboratory at Kentucky State University. Mr. McKay also entered historical fish disease diagnostic records into digital Excel spreadsheets (along with John Kelso, Extension & Research Assistant). Casework has also been performed and entered into the database by another graduate student Tifani Watson. PD Durborow, Extension & Research Assistant John Kelso, and graduate students Tyler McKay and Tifani Watson have also begun entering data into a preliminary edition of the Database that is still under development by Apax, Inc. In addition, undergraduate students at Oregon State University, continued creating computer learning games under the direction of Co-PI Dr. Stephen Atkinson.
Publications
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Progress 05/01/19 to 04/30/20
Outputs Target Audience:During Aquaculture Europe 2019 in Berlin, Germany October, at the combined poster session and trade show arena, valuable contacts were made with key target audiences that were exhibiting at the trade show. These included a Greek i.t. company led by Vasailis Foteinos working on IMPAQT (Intelligent Management System for Integrated Multi-trophic Aquaculture); Observations Medical Outcomes Partnership (OMOP) Database via Neha Mandadi; INVE (Mayleen Chang Vasquez) developing a database for their fish disease diagnostics records at their Thai facility; Rosa Merino of Hipra interested in the database for their vaccine production in Catalonia, Spain; Biomin (Dr. Silvia) for their disease/nutrition database; Shane Hunter, owner of Aqua Bio Tech in Malta, working with Dr. Tomas on the iFishIENCi database; and Dr. Ariadna Sitja-Bobadilla, project director for ParaFishControl database that tracts disease occurrence among aquacultured animals in ocean net-pens. These seven organizations/target audiences expressed interest in collaborating with us upon completion of the Clinical Fish Health Database. In addition, during the week preceeding the conference, the grant PD made visits to target audiences in Hannover and Munich, Germany; at Hannover University School of Veterinary Medicine and at Technical University of Munich. Collaborations were discussed with Dr. Deiter Steinhagen, of Hannover University and Dr. Ralph Kuehn at TUM (Munich, Germany). Other target audiences include fish disease diagnostic laboratories in the U.S. including Louisiana State University School of Veterinary Medicine and the Alabama Fish Farming Center in Marion, Alabama (a division of Auburn University). Private veterinarian Dr. Craig Blair who has a veterinary medicine practice in Lexington, Kentucky, has also agreed to collaborate on data collection using the Database. And, Kentucky State University and Oregon State University students will be involved in beta testing of the Database, serving as an important target audience. Changes/Problems:The Database development by Apax has proceeded slower than anticipated, so a first-year no-cost extension is/was needed. What opportunities for training and professional development has the project provided?Plans for the PD and Co-PD to travel to each other's laboratories was not carried out due to the pandemic. But pre-pandemic travel/professional development was made by PD Durborow to Aquaculture Europe in October 2019 to interact with potential collaborators on the project in Germany, Greece, Catalonia, Spain, Thailand, and Malta. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?Plans are in place to continue developing the Clinical Fish Health Database (by Kentucky State University with Apax, Inc.) as well as to continue developing the computer learning games (by Oregon State University). Data collection of fish disease diagnostic cases will continue.
Impacts What was accomplished under these goals?
Extension and research associate Ashmita Poudel has been transcribing historical fish disease records into Excel format that will eventually be put into the Database platform that is being developed. Apax, Inc., a software, app and database-creating company in Lexington, Kentucky, was approved by the Kentucky State University Board of Regents and then the Kentucky Legislative Committee on Contracts. They were approved by KSU BOR (Kentucky State University Board of Regents) in December and by LCoC (Kentucky's Legislative Committee on Contracts) in January 2020. Graduate student Tyler McKay (beginning in August 2019) is working under funding from this grant. He has been involved in diagnosing fish diseases during this quarter including necropsy procedures to determine cause of death as well as biochemical identification of bacteria causing disease and antibiotic susceptibility testing to determine the best treatment of medicated feed. Fish parasites and water quality problems associated with the disease cases were also identified; proper treatments for each were recommended to clientele using our fish disease diagnostic laboratory at Kentucky State University. He also entered historical fish disease diagnostic records into digital Excel spreadsheets (along with Ashmita Poudel, Extension Associate). Casework has also been performed and entered into the database by another graduate student Tifani Watson. In addition, undergraduate students at Oregon State University, were involved in creating computer learning games under the direction of Co-PI Dr. Stephen Atkinson.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2019
Citation:
Durborow, R.M., S. Atkinson, A. Poudel, T. McKay, and A. Sabek. 2019. Developing a clinical database, mobile application (app), and learning games for fish disease diagnostics. Poster presented at: Aquaculture Europe 2019, Berlin, Germany, October 7 10.
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Progress 05/01/18 to 04/30/19
Outputs Target Audience:The specific target audiences for this project and the App are KSU graduate and undergraduate students, commercial aquaculturists, aquaculture researchers, recreational pond owners, fish hobbyists, and veterinarians, including Dr. Craig Blair, DVM, a small-animal veterinarian interested in diagnosing and treating fish diseases in Lexington, Kentucky. The target audience for the database will be fish disease diagnostic laboratories nationwide and perhaps globally. The learning games are being designed for a general audience and should have wide appeal for school and university students, fish health profession trainees, and the general public. Our goal is to grow interest in fish care and fish diseases among students and the general public currently not involved in that field. Changes/Problems:The contract with Cambrian Solutions was terminated due to lack of progress by this company in building our database and app. The bidding process to find a replacement company is proceeding. What opportunities for training and professional development has the project provided?International graduate students Adham Sabek (Kuwait) and Ashmita Poudel (Nepal) have been trained in fish health management through experience in the Kentucky State University (KSU) Fish Disease Diagnostic Laboratory (FDDL) and by digitally recording fish disease clinical cases into Excel spreadsheets during database building. The database and app, when complete, will be ideal for training and development (for students as well as young professionals needing to acquire expertise in this field, including veterinarians wanting to expand into aquaculture-related health). Adham, in particular, acquired IT and business skills during communications with Cambrian Solutions, the company formerly building the database and app. At OSU, the learning game design and creation has provided important capacity building for Masters student training, as a real-world exercise in client liaison, and game design and user testing, which aligns very well with Professor Zhang's design program for graduate students. Similarly, the artwork requirements for the learning games are teaching the undergraduate artist (Myers) to deliver a wide range of designs with deadlines for a real-world product, not just a study exercise. How have the results been disseminated to communities of interest? Progress and plans for this USDA CBG were presented at the 2019 World Aquaculture Society Annual Meeting in New Orleans during a fish health session chaired by Kathleen Hartman, DVM, PhD. Other key scientists at this presentation included Kevin Snekvik, DVM, PhD, Fish Disease Diagnostic Laboratory Director at WADDL (Washington Animal Disease Diagnostic Laboratory), part of Washington State University in Pullman, WA; David Straus, PhD, Fish Disease Researcher at ARS/USDA Harry K. Dupree Stuttgart National Aquaculture Research Center; and Guppy Blair, DVM, PhD, U.S. Fish & Wildlife Service, Bozeman, Montana. Grant information was also presented informally to fish health diagnosticians, including Larry Hanson, PhD, Mississippi State University College of Veterinary Medicine; John Hawke, PhD, Louisiana State University School of Veterinary Medicine; and William Hemstreet, Fish Health Specialist, Alabama Fish Farming Center, Greensboro, AL. Students at Kentucky State University enrolled in Fish Diseases (AQU 511 & 411) and Fish Diseases Laboratory (AQU 510 & 410) were taught about the database and app concepts. Students taking these classes in the fall 2020 semester will be part of the beta testing of the database and app (contingent on USDA approval for a one-year no-cost extension of the grant). Information on this database and app creation process was also disseminated to WAVMA during a presentation at their annual meeting in St. Kitts in fall 2018. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, we will identify a competent company to build the database and app. We will work with this company during fall 2019, winter 2019/20, and spring 2020 to have a functional database for recording clinical data from the KSU FDDL as well as from collaborating laboratories (including Mississippi State University, Louisiana State University, Alabama Fish Farming Center, and U.S. Fish & Wildlife laboratories). Iterative building of the database and app, as well as beta testing will proceed during 2020. Beta testing will be conducted by graduate and undergraduate students taking Fish Diseases (AQU 511 & 411) and Fish Diseases Laboratory (AQU 510 & 410). Kentucky State University plans to request a one-year, no-cost extension of this grant to end April 30, 2021, and will officially request this extension in early 2020. Beta testing will continue through April 30, 2021 if extension of the grant is approved. Oregon State University will continue to develop the learning games. Fish Detective is the more difficult programming task and requires the most audience/player feedback to get right, so it is being created first. The current plan is to have it fully coded through summer 2019 to have a beta version available for testing with OSU/KSU students (plus off-campus groups) in Fall term when classes resume. The second learning game, SimFish, is simpler to implement and will be created after FishDetective (in early 2020). The fish pathogen database continues to be expanded with new images, videos, and pathogen summaries created by OSU & KSU students and will be linked with the games and clinical database.
Impacts What was accomplished under these goals?
To improve organization of fish disease diagnostic laboratory clinical data, this grant was obtained to transform fish disease reports from hand-written/fill-in-the-blank paper forms to Excel spreadsheets and then to a database platform. During this reporting period, graduate student Adham Sabek entered clinical fish disease diagnostic laboratory fish kill reports into Excel. He entered 10 years of data, 2009 through 2018, with the intent of bulk downloading data into a database platform being developed concurrently by Cambrian Solutions, a software company in New Jersey. The database was being designed to contain learning machine technology and to have the capability of syncing with a mobile application (also being developed by Cambrian Solutions). Throughout this reporting period, it became apparent that Cambrian Solutions was not making satisfactory progress building the database and app. The digital product they developed since their start in the database and app production was obtained by Kentucky State University and the contract with them was terminated. Three potential companies have been identified as being able to start where Cambrian left off; KSU is in the process of obtaining bids for the job and these three companies are under consideration for the contract. On a more positive note, commitments to collaborate on the project have been obtained from the fish disease diagnostic laboratories at Mississippi State University (MSU), Louisiana State University (LSU), and Auburn University. Dr. Larry Hanson at MSU College of Veterinary Medicine in Starkville sent diagnostic laboratory results to be entered into the upcoming database and the LSU Veterinary School and the Alabama Fish Farming Center (a branch of Auburn University in Greensboro, AL) agreed to contribute cases from their diagnostic laboratories to populate the database. Kentucky State University has also been working collaboratively with Dr. David Scarfe, DVM, from the World Aquatic Veterinary Medical Association (WAVMA) and Ms. Amy Patterson of GlobalVetLink. Additionally, the concept of database and app development was presented and discussed during an aquaculture health management special session at the World Aquaculture Association Annual Meeting in New Orleans (March 2019) chaired by Dr. Kathleen Hartman, DVM, PhD, Aquaculture Program Leader for USDA APHIS. Much interest was shown for this project, including from Dr. Kevin Snekvik, Director of Operations, Pathologist & Aquatic Health Section Head and Clinical Professor at the Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA. Two weeks after this meeting, several diagnosticians participating in this session also joined a teleconference call organized by Dr. Hartman. Participation of U.S. Fish and Wildlife Service, under the direction of Dr. Joel Bader, has also been established. Future direction on these collaborations will resume when building of the database and app resume with the new company. More recently, we made a decision to use Oregon State University's www.fishpathogens.net (developed by grant Co-PD Stephen Atkinson) as a repository for fish disease videos and photographs for use on the mobile app. This will save time and money instead of us searching and paying for an independent server for this function. Co-PD Atkinson managed two student teams to expand the fish pathogens database and build the learning games. Undergraduate students Sophia Jadzak and LilyAna Folk continued to capture images of pathogens in fresh fish and scanned images from Oregon State University's historic microscope slide collection. Graduate student Kevin Hung and undergraduate student Paris Myers, together with fish parasitologist Dr Sascha Hallett and software engineer Prof. Yue Zhang, are designing and implementing the learning games. The first game, Fish Detective, has been fully designed and its artwork and computer code are being created. Design for the second game, SimFish, is 75% complete and the remainder of the design will be implemented while we are testing Fish Detective.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2019
Citation:
Mitchell, K.M. and R.M. Durborow. 2019. Largemouth bass diseases. Chapter in Largemouth Bass Aquaculture. James H. Tidwell, Shawn Coyle, & Leigh Anne Bright, editors. 5M Publishing.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Durborow, R.M. & S. Atkinson. 2019. Developing a mobile app, clinical database, and learning games for fish disease diagnostics. In: Proceedings of the World Aquaculture Society, New Orleans, LA, p 324.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Mitchell, K. & R. Durborow. 2019. Common diseases of largemouth bass. In: Proceedings of World Aquaculture Society, New Orleans, LA, p 733.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
Durborow, R.M. & S. Atkinson. 2018. Developing a mobile app, clinical database, and learning games for fish disease diagnostics. In: Proceedings of the World Aquatic Veterinary Medical Association Conference, November 9, 2018, St. Kitts.
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Progress 05/01/17 to 04/30/18
Outputs Target Audience:The specific target audiences for this project and the App are KSU graduate and undergraduate students, commercial aquaculturists, aquaculture researchers, recreational pond owners, fish hobbyists, and veterinarians, including Dr. Craig Blair, DVM, a small-animal veterinarian interested in diagnosing and treating fish diseases in Lexington, Kentucky. The target audience for the database will be fish disease diagnostic laboratories nationwide, and perhaps globally. Changes/Problems:A positive change was made to the original grant protocol. A single company, Cambrian Solutions, is now developing both the App and database for the project. Originally, they were designated to develop only the App, and a separate company, New Planet, was to develop the database. Due to difficulties in negotiations/planning after grant dollars were received, we decided not to use New Planet and to use Cambrian Solutions for both Database and App development. We view this move as a positive change, because a single company will now be developing both products, making them more compatible. Due to problems with transitions in Kentucky State University's administration and certain departments, the process in establishing Oregon State University as a grant sub-recipient, Cambrian Solutions as a sub-contractor and Dr. Julie Bebak as a consultant was exceedingly slow. All of these issues have now been overcome and the relevant accounting mechanisms are established. Accomplishments during the second reporting period are expected to exceed those of the first period. What opportunities for training and professional development has the project provided?At KSU, Kat Mitchell, Ashmita Poudel and new graduate student Adham Sabek reviewed 40 fish disease cases during this reporting period, which helped to develop their skills and experience in fish disease diagnosis as well as skills in videoing and photographing the diseases. Adham Sabek acquired some experience volunteering during this reporting period, but was officially employed in the next reporting period. At OSU, undergraduate student Sophia Jadzak was trained in microscopy of both wet mounts and fixed material and tutored on Excel database construction for pathogen slide curation. Undergraduate student LilyAna Folk was trained in fish dissection and microscopic examination of fresh tissues for pathogen detection. How have the results been disseminated to communities of interest?The project is not yet at the stage for dissemination of results, but during the fall 2018 semester a group of on-campus students enrolled in Fish Diseases Laboratory (AQU 510/410) and Lecture (AQU 511/411) will be involved in diagnosing fish diseases cases submitted to the FDDL as well as alpha-testing the App to provide critical feedback on how this product can be improved. What do you plan to do during the next reporting period to accomplish the goals?Graduate and undergraduate students enrolled in on-campus courses AQU 511/411 (Fish Diseases Lecture) and AQU 510/410 (Fish Diseases Lab) will acquire photos and video footage of cases submitted to the KSU FDDL. These are real-world cases from a variety of clients (aquaculturists, recreational pond owners, fish hobbyists, aquarium owners, aquaculture researchers, and state and federal government employees commissioned to culture game fish for stocking into wild rivers, lakes, and streams). During the fall 2018 semester, a group of on-campus students enrolled in these courses will be involved in diagnosing fish diseases cases submitted to the FDDL as well as alpha-testing the App. Co-investigator Dr. Stephen Atkinson and his Microbiology and Computer Science undergraduates at Oregon State University, consultant Dr. Julie Bebak, and App/Database development company Cambrian Solutions will continue development of the App, database and computer games through the second-year reporting period. A visual arts undergraduate student at OSU has already been interviewed to assist with graphical design of the learning games as part of an undergraduate thesis project. At both KSU and OSU, students will test the database and computer learning games to provide critical feedback on how these products can be improved iteratively as development progresses through the year.
Impacts What was accomplished under these goals?
Cambrian Solutions (part of Studio Symposium) is developing the App and database synchronously to enable both products to work as a single unit. The AI/Learning Machine component of the App will have access to the database information and will be able to learn patterns, causes and effects, and likely etiologies of new diseases encountered by App users. This is an improvement over original plans to have separate companies develop the App and database. Kentucky State University Fish Disease Diagnostic Laboratory (FDDL) fish kill data from 2017, recorded on paper forms, were entered into an Excel spreadsheet developed by KSU Extension Associate Kathryn Mitchell, consultant Dr. Julie Bebak, and the PI, Dr. Robert Durborow, and submitted to Cambrian Solutions for use in developing the database platform. Existing fish disease videos, photos, and texts were also submitted to Cambrian Solutions to begin the App/AI development. Additionally, Robert Durborow, Kat Mitchell, and graduate student Ashmita Poudel have been recording videos and photos from cases submitted to the FDDL since the grant began. At OSU, co-PD Stephen Atkinson and undergraduate students Sophia Jadzak and LilyAna Folk, have begun curating pathogen slide collections (35-mm and glass) in preparation for digitization, and capturing new images of diseased fish. These will be incorporated into the App/AI. The grant supported the purchase of Canon cameras/camcorders at both KSU and OSU to help in capturing videos and photographs.
Publications
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