Source: UNIVERSITY OF ARKANSAS submitted to NRP
INVESTIGATION OF METHODS TO IMPROVE SUSTAINABLE HEALTH IN POULTRY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
ANIMAL HEALTH
Reporting Frequency
Annual
Accession No.
1011834
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Dec 13, 2016
Project End Date
Sep 30, 2021
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
Poultry Sciences
Non Technical Summary
JUSTIFICATION: Despite clear indications of links between enteric microbiota and multiple human diseases, there is still no clear mechanism established, at least not to the level for which targeting gut microbiota for disease treatment can be reasonably successful, partly due to a lack of effective research tools. While many techniques exist for measuring effects of microflora on a host, and multiple animal models exist, the intense methodologies for maintaining gnotobiotic and knock-out models limits progress in the field. Investigations into the effects of regulation of host function by early microflora colonization has typically utilized gnotobiotic or disease models in mice. An inherent handicap of these models is a lack of natural phenotype and complications related to unintended physiologic changes. Probiotic studies in mice with pseudo-controlled enteric microflora have established some important relationships between the microbiome, inflammation, skeletal development, and obesity, to name a few. While these models provide critical information about interactions and symbiosis of the enteric microbiome and vertebrate animals, maintenance of controlled microbial environments, especially from birth, can be cumbersome, and expensive. Avian species provide a distinct opportunity, through prenatal in ovo inoculation of bacteria in order to evaluate early colonization, possibly in combination with enteric inflammation models, to combine both microbiome and immune function studies of these interactions and influences on multiple aspects of health and development. Direct inoculation of the amniotic fluid near hatch, which is swallowed by the embryo immediately prior to hatch, is easily accomplished with chicken embryos by in ovo injection. Recently, completed studies have shown that in ovo injection of chicken eggs at 18d of incubation can markedly influence recoverable bacteria from the GIT 48h after hatch (manuscript in preparation). These experiments did not include any attempts to decrease inoculation with indigenous microflora during the early postnatal period. We believe that modification and extension of this existing protocol can greatly enhance the ability to effectively study host-microbiome interactions and provide for a robust model towards understanding the role of specific pioneer colonizers of the intestinal tract of neonates. This research will contribute to scientific knowledge or public welfare.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31132991103100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3299 - Poultry, general/other;

Field Of Science
1103 - Other microbiology;
Goals / Objectives
Bacterial communities living and colonizing in the gastrointestinal tract of animals outnumber total somatic cells of metazoans by an estimated 10-fold [1]. Today, the microbiome is recognized as the 'forgotten organ,' operating like an organ within the host and orchestrating numerous physiological and biological functions that have a profound impact on the balance between health and disease [2]. Essential colonization of these bacterial populations starts at birth/hatch, and is followed by progressive assembly of a complex and dynamic microbial society [3]. Under commercial conditions, millions of chickens and turkeys hatch in a hostile environment, and are exposed for several hours to heat stress and potential pathogenic bacteria in the hatcheries. Increased stress along with the potential abundance of pathogens in the hatching cabinet leads to ideal conditions for pathogen colonization. It is generally accepted that the natural route of transmission of zoonotic pathogens such as Salmonella, is fecal-oral [4]. However, published studies from our laboratory have also suggested that airborne transmission of Salmonella in poultry is possible [5]. This mode of infection could explain some clinical expression of relatively low-dose infectivity under field conditions in relation to the high oral challenge dose that is typically required for infection through the oral route in laboratory studies. This also supports previous studies demonstrating fan driven spread of Salmonella within the hatching cabinet and hatchery incubators [6]. Modern poultry production has separated the newly hatched chick from the hen and the complex microbiota that is maternally transmitted. Chicks are now born into the sanitized environment of the hatching cabinet where the first gut colonizers are often pathogens such as E. coli or Salmonella. Before hatching, the environment within the egg exists in a sterile state. In ovo application of probiotics may allow colonization of beneficial bacteria before the chick is ever exposed to pathogens within the hatching environment. In this research, multiple lactic acid bacteria (LAB) isolates will be screened for their impact on hatchability and their ability to colonize the gut after in ovo application. Hence, the objectives for this proposal are:To evaluate the effect of the in ovo administration of FloraMax®-B11 on MD vaccine herpesvirus of turkeys (HVT) protective efficacy, hatchability, microbiota composition, morphometric analysis, and SE infection in chickens. These results will provide preliminary data that can be used to leverage funding for investigation into the effect of GIT microbiome manipulation on immune function profiles. Overall, this model will provide a means by which to study effects of microflora on multiple health parameters, as well as population manipulation strategies towards favorable enteric microbe conditions that promote a symbiotic relationship.Incubation is a crucial step in a chicken's life cycle, and the environment in which an embryo develops can have lasting effects on the bird's well-being throughout its life. It has been documented that temperature, humidity, sound, and light can all impact development of an embryo. Stress hormones have been associated to increase intestinal permeability. Therefore, a second objective for this grant is to evaluate the effect that heat stress or cold stress has on inflammation and intestinal permeability and susceptibility to yolk sac infection and bacteremia. In these trials, hatchability, microbiota composition, and morphometric analysis will be also evaluated.3. To evaluate the administration of probiotic bacteria sprayed in hatching cabinets as an alternative to formaldehyde to sanitize and provide early colonization with beneficial bacteria to the hatching chickens. For these experiments, incidence of Salmonella, microbial composition in the gastrointestinal tract, metagenomics analysis, gut permeability, and serum biomarkers (citrulline, MUC2, IFN gamma, superoxide dismutase and total IgA) will be compared between chickens hatch in an environment where formaldehyde has been used versus spraying of probiotic bacteria.
Project Methods
PROCEDURES: Our central hypothesis that prenatal enteric inoculation of selected bacterial isolates, via amnionic injection prehatch, will manipulate pioneer colonization of the GIT and altered host GIT/immune responses will be tested by addressing the following:We will evaluate the effect of the in ovo administration of FloraMax®-B11 on MD vaccine herpesvirus of turkeys (HVT) protective efficacy, hatchability, microbiota composition, morphometric analysis, and SE infection in chickens. Potential interference with MD vaccine will be done incollaboration with Dr. Hans Chen at ADOL and will include effect on PFU and resistance to challenge in vaccinated birds. Microbiota composition will be performed by high throughput sequencing and description of relative abundance through the genus level in all cases. Morphometric analysis will be performed as previously described from our laboratory (Menconi et al., 2015).Incubation is a crucial step in a chicken's life cycle, and the environment in which an embryo develops can have lasting effects on the bird's well-being throughout its life. It has been documented that temperature, humidity, sound, and light can all impact development of an embryo. Stress hormones have been associated to increase intestinal permeability. Therefore, a second objective for this grant is to evaluate the effect that heat stress or cold stress has on inflammation and intestinal permeability and susceptibility to yolk sac infection and bacteremia. In these trials, hatchability, microbiota composition, morphometric analysis will be also evaluated. These parameters will be determined as described above.To evaluate the administration of probiotic bacteria sprayed in hatching cabinets as an alternative to formaldehyde to sanitize and provide early colonization with friendly bacteria to the hatching chickens. For these experiments, incidence of Salmonella, microbial composition in the gastrointestinal tract, metagenomics analysis, gut permeability, and serum biomarkers (citrulline, MUC2, IFN gamma, superoxide dismutase and total IgA will be compared between chickens hatch in an environment where formaldehyde has been used versus spraying of probiotic bacteria.While we have obtained significant differences in FITC-d intestinal permeability, we believe we can optimize the ELISA method to obtain more robust windows between control or treated chickens. Hence, one of our priorities will be working in the optimization technique. Ongoing studies are evaluating dosage, timing and the optimal MW of the dextran-FITC conjugate.The mucosal surfaces (gastro intestinal, respiratory, urogenital, ocular and inner ear) represent a very large exposure area to exogenous agents including microorganisms. As such, these anatomical sites are the main areas of interaction of the body with the external environment and thus with potential pathogens. The mucosal associated lymphoid tissue (MALT), differs fundamentally from Systemic immune responses in that: the major isotype in mucosal secretions is secretory, IgA and most of the antibody-producing cells and effector T cells occur in the MALT. Interestingly, the gut associated lymphoid tissue (GALT), as part of the MALT, is the largest immunological organ in the body, comprising 70-80 % of all immunoglobulin producing cells. GALT produces more secretory IgA (50-100 mg/kg body weight/day) than the total production of IgG in the body (30 mg/kg body weight/day)[21]. Hence, during this coming year, we will focus on an ELISA method for quantification total and specific IgA from different chicken MALT tissues.

Progress 12/13/16 to 09/30/21

Outputs
Target Audience:During the life of this project, substantial progress was made during this time period, exceeding 30+ refereed publications, book chapters, and presentations at national scientific forums. Numerous contacts and meetings with direct poultry integrators and allied industry scientists were held. The research provided improved approaches for management of numerous problems related to sustainable poultry health for the poultry industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Promoted collaboration with other scientists and provided opportunities for students and technicians to learn new skills. How have the results been disseminated to communities of interest?Publications, conference proceeding, invited lectures. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Developed and validated laboratory-scale challenge models to evaluate alternatives to formaldehyde fumigation Optimized assays to assess gut permeability and effects of treatments on early performance Conducted several studies related to heat stress, probiotics (in ovo and spray applied), etc. Evaluated routes of administration - E. coli and Salmonella

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: 15. Makenly E. Coles, Roberto Se�as-Cuesta, B. Danielle Graham, Callie M. Selby, �lvaro J. Uribe, Blanca C. Mart�nez, Jamie A. Angel-Isaza, Christine N. Vuong, X. Hernandez-Velasco, Billy M. Hargis, and Guillermo Tellez-Isaias. Assessment of essential oil formulations on growth performance, intestinal barrier integrity, and lesions in broiler chickens using a necrotic enteritis laboratory challenge model. International Poultry Scientific Forum. January 25-26, 2021. Virtual meeting. P170.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: G. Tellez Jr., M. A. Arreguin-Nava, J. A. Maguey, M. A. Michel, J. D. Latorre, R. Merino-Guzman, X. Hernandez-Velasco, P. A. Moore Jr., B. M. Hargis, G. Tellez-Isaias. 2020. Effect of Bacillus-DFM on leaky gut, serum peptide YY concentration, bone mineralization, and ammonia excretion in neonatal turkey poults fed with a rye-based diet. Poultry Science. 99:45144520.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: A. Morales-Mena, S. Mart�nez-Gonz�lez, K. D. Teague, L. E. Graham, R. Se�as-Cuesta, C. N. Vuong, H. Lester, D. Hernandez-Patlan, B. Solis-Cruz, B. Fuente-Martinez, X. Hernandez-Velasco, B. M. Hargis, G. Tellez-Isaias. 2020. Assessment of fermented soybean meal on Salmonella Typhimurium infection in neonatal turkey poults. Animals. 10, 1849; doi:10.3390/ani10101849.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: C. N. Vuong, V.A. Kuttappan, O. B. Faulkner, L.R. Berghman, A. D. Wolfenden, Tellez-Isaias, G., Jonas, M., Kapczynski, D.R., Hargis, B.M. and Bielke, L.R., 2020. Comparison of oil emulsion, mannosylated chitosan, and Bacillus vector adjuvants for vaccination against influenza in chickens. Journal of Applied Poultry Research, 29(3): 653-664. https://doi.org/10.1016/j.japr.2020.04.003
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: L. Laverty, R. Se�as-Cuesta, S. Mart�nez-Gonz�lez, C. Selby, G. Tellez-Jr, X. Hernandez-Velasco, C. Vuong, B. Hargis, G. Tellez-Isaias, D. Graham. 2020. Evaluation of oocyst shedding of Eimeria maxima and Eimeria acervulina in broiler chickens. Abanico Veterinario. 10:1-11. http://dx.doi.org/10.21929/abavet2020.20
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: J. Ruff, G. Tellez Jr., A. J. Forga, R. Se�as-Cuesta, C. N. Vuong, E. S. Greene, X. Hernandez-Velasco, A. J. Uribe, B.C. Mart�nez, A. J. Angel-Isaza, S. Dridi, Clay J. Maynard, Casey M. Owens, Billy M. Hargis and Guillermo Tellez-Isaias. 2021. Evaluation of Three Formulations of Essential Oils in Broiler Chickens under Cyclic Heat Stress. Animals 2021, 11, 1084. https://doi.org/10.3390/ani11041084
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: J. Ruff, T. L. Barros, J. Campbell, R. Gonz�lez-Esquerra, C. N. Vuong, S. Dridi, E. S. Greene, X. Hernandez-Velasco, B. M. Hargis, and G. Tellez-Isaias. 2021. Spray-Dried Plasma Improves BodyWeight, Intestinal Barrier Function, and Tibia Strength during Experimental Constant Heat Stress Conditions. Animals. 11, 2213. https://doi.org/10.3390/ani11082213
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: B. D. Graham, C. M. Selby, L. E. Graham, K. D. Teague, G. Tellez-Isaias, B. M. Hargis, and C. N. Vuong. 2021. Development of a wild-type Escherichia coli environmental bloom model to evaluate alternatives to formaldehyde fumigation. Poultry Science. 100:100975. https://doi.org/10.1016/j.psj.2020.12.072
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: C. M. Selby, B. D. Graham, L. E. Graham, K. D. Teague, B. M. Hargis, G. Tellez-Isaias, B. M. Hargis, and C. N. Vuong. 2021. Research Note: Application of an Esherichia coli spray challenge model for neonatal broiler chickens. Poultry Science. 100:100988. https://doi.org/10.1016/j.psj.2021.01.011
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: M. E. Coles, A. J. Forga, R. Se�as-Cuesta, B. D. Graham, C. M. Selby, �. J. Uribe, B. C. Mart�nez, J. A. Angel-Isaza, C. N. Vuong, X. Hernandez-Velasco, B. M. Hargis and G. Tellez-Isaias. 2021. Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens. Animals 2021, 11, 1111. https://doi.org/10.3390/ani11041111
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: C. N. Vuong, G. J. Mullenix, M. T. Kidd, W. G. Bottje, B. M. Hargis, and G. Tellez-Isaias. 2021. Modified serum fluorescein isothiocyanate dextran (FITC-d) assay procedure to determine intestinal permeability in poultry fed diets high in natural or synthetic pigments. Poultry Science 100:101138 https://doi.org/10.1016/j.psj.2021.101138
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: A. M. Ashcraft, M. E. Coles, L. C. Beer, B. D. M. Graham, G. Tellez-Isaias, B. Wooming and B. M. Hargis. 2021. Research note: Fate and dissemination of Salmonella enterica serovar Reading in turkeys at processing using an oral gavage challenge model. Poultry Science, 100:101114. https://doi.org/10.1016/j.psj.2021.101114
  • Type: Book Chapters Status: Published Year Published: 2021 Citation: Evaluation of Bone Marrow Adipose Tissue and Bone Mineralization on Broiler Chickens Affected by Wooden Breast Myopathy Barbara de Almeida Mallmann, Elizabeth M. Martin, Kyung Soo Kim, Norma L. Calderon-Apodaca, Mikayla F. A. Baxter, Juan D. Latorre, Xochitl Hernandez-Velasco, Leopoldo Paasch-Martinez, Casey M. Owens, Sami Dridi, Walter G. Bottje, Elizabeth S. Greene and Guillermo Tellez-Isaias. 2021. In: Velleman, S. G., Petracci, M., eds. Avian Muscle Development and Growth Mechanisms: Association With Muscle Myopathies and Meat Quality. Lausanne: Frontiers Media SA. doi: 10.3389/978-2-88966-314-9
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: 11. Guillermo Tellez-Isaias, Daniel Hernandez-Patlan, Bruno Solis-Cruz, Victor Petrone-Garcia, Ana Leyva-Diaz, Christine Vuong, Danielle Graham, Callie Selby, Juan Latorre, and Billy Hargis. Studies conducted at the University of Arkansas to evaluate curcumin as a feed additive to control bacterial and protozoal infections and reduce aflatoxicosis severity in poultry. Symposium on Gut Health. October 31-November 3, 2021. St. Louis, Missouri. 105
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: 13. Aaron J. Forga, B. Danielle Graham, Makenly Coles, Lesleigh Beer, Callie Selby, Lucas E. Graham, Roberto S. Cuesta, Jared Ruff, Guillermo Tellez-Isaias, Billy M. Hargis, and Christine Vuong. Effect of Clostridium septicum hemolytic activity units on Clostridial dermatitis (cellulitis) bacterin/toxoid humoral immunogenicity in turkeys. Poultry Science Association. July 19-22, 2021. Virtual meeting. 349P
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: 14. Callie Selby, Danielle Graham, Lucas Graham, Aaron Forga, Mikayla Baxter, Guillermo-Tellez- Isaias, Billy M. Hargis, and Christine Vuong. Effect of oral or intracloacal challenge with Escherichia coli or Salmonella Enteritidis on enteric colonization and early performance in broiler chickens. Poultry Science Association. July 19-22, 2021. Virtual meeting. 350P


Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Substantial progress was made during this time period, with more than 20 refereed publications and 30 presentations at national meetings. Numerous contacts and meetings with direct poultry integrators and allied industry scientists were held. The research provided improved approaches for management of numerous problems related to sustainable poultry health for the poultry industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Promoted collaboration with other scientists and provided opportunities for students and technicians to learn new skills. How have the results been disseminated to communities of interest?Publications, conference proceeding, invited lectures. What do you plan to do during the next reporting period to accomplish the goals?Expand the investigation related to the objectives stated above.

Impacts
What was accomplished under these goals? Developed models to evaluate alternatives to formaldehyde fumigation, such as probiotics Optimized assays to assess gut permeability Conducted several studies related to heat stress, probiotics (in ovo and spray applied), etc.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Snyder, R.P., Guerin, M.T., Hargis, B.M., Page, G. and Barta, J.R., 2020. Monitoring coccidia in commercial broiler chicken flocks in Ontario: Comparing oocyst cycling patterns in flocks using anticoccidial medications or live vaccination. Poultry Science
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Snyder, R.P., Guerin, M.T., Hargis, B.M., Kruth, P.S., Page, G., Rejman, E., Rotolo, J., Sears, W., Zeldenrust, E.G., Whale, J. and Barta, J.R., 2020. Restoration of anticoccidial sensitivity to a commercial broiler chicken facility in Canada. Poultry Science.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Bruno Solis-Cruz, Daniel Hernandez-Patlan, Victor M. Petrone, Karine P. Pontin, Juan D. Latorre, Eric Beyssac, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Margarita A. Arreguin, Billy M. Hargis, Raquel Lopez-Arellano, and Guillermo Tellez-Isaias. 2019. Evaluation of a Bacillus-based direct fed microbial on aflatoxin B1 toxic effects, performance, immunological status and serum biochemical parameters in broiler chickens. Avian Diseases. 63:659-669
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lesleigh Beer, Vuong C.N., Barros T., Latorre J.D., G. Tellez-Isaias, Fuller L., Hargis B. M. 2019. Evaluation of boric acid as a chemoprophylaxis candidate to prevent histomoniasis. Poultry Science. 99:19781982.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: K.M. Feye, M.F.A. Baxter, G. Tellez, M.H. Kogut, and S.C. Ricke. 2020. Influential Factors on the Composition of the Conventionally Raised Broiler Microbiomes. Poultry Science. 99:653659.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Jared Ruff, Thaina L. Barros, Guillermo Tellez Jr., Justin Blankenship, Howard Lester, Brittany D. Graham, Callie A.M. Selby, Christine N. Vuong, Sami Dridi, Elizabeth S. Greene, X. Hernandez-Velasco, Billy M. Hargis, and Guillermo Tellez-Isaias. 2020. Evaluation of a heat stress model to induce gastrointestinal leakage in broiler chickens. Poultry Science. 99:16871692.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Vuong, C.N., Kuttappan, V.A., Faulkner, O.B., Berghman, L.R., Wolfenden, A.D., Tellez-Isaias, G., Jonas, M., Kapczynski, D.R., Hargis, B.M. and Bielke, L.R., 2020. Comparison of oil emulsion, mannosylated chitosan, and Bacillus vector adjuvants for vaccination against influenza in chickens. Journal of Applied Poultry Research, 29(3): 653-664. https://doi.org/10.1016/j.japr.2020.04.003
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Barros, T.L., Beer, L.C., Tellez, G., L. Fuller, Hargis, B.M., Vuong, C.N. 2020. Evaluation of Dietary Administration of Sodium Chlorate and Sodium Nitrate for Histomonas meleagridis prophylaxis in turkeys. Poultry Science. 99:19831987.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Mikayla F. A. Baxter, Elizabeth S. Greene, Michael T. Kidd, Guillermo Tellez-Isaias, Sara Orlowski, and Sami Dridi. 2020. Water amino acid chelated trace mineral-supplementation decreases circulating and intestinal HSP70 and pro-inflammatory cytokine gene expression in heat-stressed broiler chickens. Journal Animal Science 98(3), p.skaa049.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Margarita A. Arreguin-Nava, Brittany D. Graham, Bishnu Adhikari, Melissa Agnello, Callie M. Selby, Xochitl Hernandez-Velasco, Christine N. Vuong, Bruno Solis-Cruz, Daniel Hernandez-Patlan, Juan D. Latorre, Guillermo Tellez Jr., Billy M. Hargis and Guillermo Tellez-Isaias. 2020. In ovo administration of defined lactic acid bacteria previously isolated from adult hens induced variations in the cecae microbiota structure and Enterobacteriaceae colonization on a virulent E. coli horizontal infection model in broiler chickens. Frontiers in Veterinary Sciences. 7:489. doi: 10.3389/fvets.2020.00489
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Lauren Laverty, Roberto Se�as-Cuesta, Sergio Mart�nez-Gonz�lez, Callie Selby, Guillermo Tellez-Jr, Xochitl Hernandez-Velasco, Christine Vuong, Billy Hargis, Guillermo Tellez-Isaias, Danielle Graham. 2020. Evaluation of oocyst shedding of Eimeria maxima and Eimeria acervulina in broiler chickens. Abanico Veterinario. 10:1-11. http://dx.doi.org/10.21929/abavet2020.20
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: G. Tellez Jr., M. A. Arreguin-Nava, J. A. Maguey, M. A. Michel, J. D. Latorre, R. Merino-Guzman, X. Hernandez-Velasco, P. A. Moore Jr.,? B. M. Hargis, G. Tellez-Isaias. 2020 Effect of Bacillus-DFM on leaky gut, serum peptide YY concentration, bone mineralization, and ammonia excretion in neonatal turkey poults fed with a rye-based diet. Poultry Science. 99:45144520.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Saeed El-Ashram, Yu Zhang, Yongsheng Ji, Dina Salama, Kun Mei, Li Zhili, Huang Shujian, Haoji Zhang, Shawky M. Aboelhadid, Reem A. Alajmi, Dina M. Metwally, Manal F. El-Khadragy, Billy M. Hargis, Guillermo Tellez-Isaias, Beniamino T. Cenci-Goga Musafiri Karama, Munyaradzi C. Marufu, Fathi Abouhajer, Gamal Ali Abdelhafez Hamady, Abeer El Wakil, Ibrahim Al Nasr, Xun Suo. 2020. A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites. Veterinary Medicine and Science. 00:15. DOI: 10.1002/vms3.364
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Arantxa Morales-Mena, Sergio Mart�nez-Gonz�lez, Kyle D. Teague, Lucas E. Graham, Roberto Se�as-Cuesta, Christine N. Vuong, Howard Lester, Daniel Hernandez-Patlan, Bruno Solis-Cruz, Benjamin Fuente-Martinez, Xochitl Hernandez-Velasco, Billy M. Hargis, Guillermo Tellez-Isaias. 2020. Assessment of fermented soybean meal on Salmonella Typhimurium infection in neonatal turkey poults. Animals. 10, 1849; doi:10.3390/ani10101849.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Guillermo Tellez, Barbara de Almeida Mallmann, Elizabeth Martin, Norma Calderon-Apodaca, Billy Hargis, Casey Owens.Assessment of bone marrow adipose tissue and bone mineralization on broiler chickens affected by wooden breast myopathy. 2020. International Poultry Scientific Forum. January 27-28, 2020. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Danielle Graham GS, Callie Selby, Lucas Graham, Kyle Teague, Christine Vuong, Guillermo Tellez-Isaias, Billy Hargis. 2020. Development of a wild-type Escherichia coli environmental. bloom model to evaluate alternatives to formaldehyde fumigation. January 27-28, 2020. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Aaron Forga, B. Danielle Graham, Lucas Graham, Kyle Teague, Christine Vuong,Guillermo Tellez-Isaias, Billy Hargis. 2020 Optimization of a Clostridium septicum bacterin-toxoid as costeffectiveprophylactic for Turkey Cellulitis/Dermatitis. January 27-28, 2020. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Mohit BansalGS, Ying Fu, Ayidh Almansour, Tahrir Aleneji, Mussie T. Abraha, Danielle Graham, Anamika Gupta, Hong Wang, Billy M. Hargis, Xiaolun Sun. Mammalian target of rapamycin signaling modulates chicken necrotic enteritis. July 2022, 2020. PSA Virtual Annual Meeting
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Jared Ruff, Thaina Barros, Joy Campbell, Ricardo Gonz�lez-Esquerra, Christine Vuong, Sami Dridi, Elizabet Greene, Billy Hagtis, Guillermo Tellez. 2020. Effect of feeding spray-dried plasma in broilers raised under heat stress conditions. January 27-28, 2020. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Thaina L. Barros, John Barta, Billy M. Hargis, Christine Vuong. Poultry Science Association Annual Meeting. 2020. Evaluation of the interaction between Eimeria adenoeides or E. tenella with Histomonas meleagridis in poults. July 2022, 2020. PSA Virtual Annual Meeting
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Mohit BansalGS*1, Ying Fu2, Ayidh Almansour, Mussie T. Abraha, Tahrir Aleneji, Danielle Graham, Anamika Gupta1, Hong Wang, Rohana Liyanage, Billy M. Hargis, Xiaolun Sun. 2020. Evaluation of bile acids against chicken necrotic enteritis. July 2022, 2020. PSA Virtual Annual Meeting
  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Ying FuGS, Mohit Bansal, Ayidh Almansour, Tahrir Alenezi, Mussie T. Abraha, Hong Wang, Danielle Graham, Billy M. Hargis, Xiaolun Sun. 2020. Clostridium perfringens vaccine prevents chicken necrotic enteritis. July 2022, 2020. PSA Virtual Annual Meeting
  • Type: Book Chapters Status: Published Year Published: 2020 Citation: Juan D. Latorre, Bishnu Adhikari, Si Hong Park, Kyle D. Teague, Lucas E. Graham, Brittany D. Mahaffey, Mikayla F.A. Baxter, Xochitl Hernandez, Young Min Kwon, Steven C. Ricke, Lisa Bielke, Billy M Hargis, Guillermo Tellez. 2020. Evaluation of the epithelial barrier function and ileal microbiome in an established necrotic enteritis challenge model in broiler chickens. In?: The animal microbiome in health and disease. Frontiers Media SA. Edited by?: Timothy J. Johnson and Paul Plummer. DOI 10.3389/978-2-88963-900-7


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Our target audience continutes to be the Poultry Industry and its needs. As regulations increase and antibiotics become less avaliable, we are working to find affordable and effacious products that meet the needs of poultry producers throughout the world. We have worked with numerous poultry integrators and major players in the poultry allied industry to try and meet these goals. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project assisted in the graduation of 1 PhD student during the last year, and has provided opportunities for one postdoctoral associate and one scientist from soft funding sources. Moreover, several undergraduate students have been exposed to our area of agricultural research, some may continue their studies following graduation. How have the results been disseminated to communities of interest?Journal article, presentations with abstracts and invited presentations internationally are listed. Numerous face to face meetings with industry leaders. What do you plan to do during the next reporting period to accomplish the goals?We will continue to work toward sustainable non-antibiotic solutions to ongoing and emerging poultry health issues.

Impacts
What was accomplished under these goals? Substantial progress toward these goals is documented in the journal articles, presentations with abstracts and invited presentations internationally.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Vuong, C.N., Chou W.K., Hargis B.M., Bielke L.R., Bergman L. R. CD40-targeted immunization as a method for rapid antiserum production and epitope mapping. International Veterinary Immunology Symposium. Seattle, WA. August 13-16, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Ruff J., Barros T. L., Cambell J., Gonzalez-Esquerra R., Graham B. D., Selby C. M., Vuong C. N., Dridi S., Greene E., Hargis B. M., Tellez, G. Evalutation of feeding spray-dried plasma during heat stress on broiler growth, intestinal permeability, and bone mineralization. Symposium on Gut Health in Production of Food animals. St. Louis, MO. November 3-5, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Graham L. E., Selby C. M., Graham B. D., Teague K. D., Vuong C. N., Tellez G., Hargis B. M. Evaluation of the effects of a virulent Escherichia coli seeder challenge model on selected enteric bacterial recovery and 7-day performance. World Veterinary Poultry Association Congress. Bangkok, Thailand. September 16-20, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Teague K. D., Grahm B. D., Selby, C. M., Graham, L. E., Vuong C. N., Tellez, G., Hargis B. M. In ovo administration of wild-type Escherichia coli: Effects on horizontal transmission, hatchability, and early performance on broiler chickens. World Veterinary Poultry Association Congress. Bangkok, Thailand. September 16-20, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Graham B. D., Selby C. M., Graham L. E., Teague K. D., Vuong C. N., Tellez G., Hargis B. M. Effect of norepinephrine on Salmonella Enteritidis growth in vitro and infectivity in broiler chickens. World Veterinary Poultry Association Congress. Bangkok, Thailand. September 16-20, 2019
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Solis-Cruz B., Hernandez-Patlan D., Petrone V. M., Pontin K. P., Latorre J. D., Heyssac E., Hernandez-Velasco X., Merino-Guzman R., Owens C., Hargis B. M., Lopez-Arellano R., Tellez G. 2019. Evaluation of Cellulosic Polymers and Curcumin to Reduce Aflatoxin B1 Toxic Effects on Performance, Biochemical, and Immunological Parameters of Broiler Chickens. Toxins. Vol 11, Issue 2.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Baxter M. F. A, Dridi S., Koltes D. A, Latorre J. D., Bottje W. G., Greene L. S., Bickler S. W., Kim J. H., Merino-Guzman R., Hernandez-Velasco X., Anthony N. B., Hargis B. M, Tellez G. 2019. Evaluation of Intestinal Permeability and Liver Bacterial Translocation in Two Modern Broilers and Their Jungle Fown Ancestor. Frontiers in Genetics. Vol.10:480
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Graham, B. D., Robbins, K. M., Teague K. D., Graham, L. E., Merino-Guzman R., Tellez, G., Hargis B. M. 2019. Evaluation of the efficacy of a candidate turkey cellulitis/dermatitis oil emulsion vaccine on immune response and mortality under laboratory and commercial conditions. The Journal of Applied Poultry Research.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Baxter M. F. A., Latorre J. D., Dridi S., Merino-Guzman R., Hernandz-Velasco X., Hargis B. M., Tellez G. 2019. Identification of Serum biomarkers for Intestinal Integrity in a Broiler Chicken Malabsorption Model. Frontiers Veterinary Science., Vol. 6:144.
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: B. D. Graham, C. M. Selby, K. D. Teague, L. E. Graham, C. N. Vuong, J. D. Latorre, G. Tellez, and B. M. Hargis. Development of a novel in ovo challenge model for virulent Eschericia coli strains. Submitted to Poultry Science, January 2019.
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Ruff J., Barros T.L., Tellez G., Blankenship J., Lester H., Graham B.D., Selby C.M., Vuong C.N., Dridi S., Greene E.S., Hernandez-Velasco X., Hargis B.M., and Tellez-Isaias G. Evaluation of a heat stress model to induce gastrointestinal leakage in broiler chickens. Accepted by Poultry Science.
  • Type: Journal Articles Status: Accepted Year Published: 2019 Citation: Beer L.C., Vuong C.N., Barros T.L., Latorre J.D., Tellez-Isaias G., Fuller A.L., and Hargis B.M. Evaluation of boric acid as a chemoprophylaxis candidate to prevent histomoniasis. Accepted by Poultry Science.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Vuong C. N., Coles, M. E., Graham L. E., Teague K. D., Graham B. D., Baxter M. F. A., Barros T., Latorre J. D., Tellez, G., Sellers H. S., Garritty C., Hargis, B. M. Autogenous inactivated vaccine and research techniques developed for use against inclusion body hepatitis. International Poultry Scientific Forum. Atlanta, GA. February 11-12, 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Graham, B. D., Selby C. M., Teague, K. D., Graham, L. E., Vuong C. N., Latorre J. D., Tellez, G., Hargis, B. M. Utiliation of sub-lethal bacteriostatic tetracycline co-administration for development of a novel in ovo challenge model for virulent Escherichia coli strains. International Poultry Scientific Forum. Atlanta, GA. February 11-12, 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Beer L. C., Latorre J. D., Tellez G., Hargis B. M., Vuong C. N. Evaluation of a candidate live-attenuated histomoniasis vaccine. 6th Edition of the International Conference of Poultry Intestinal Health. Rome, Italy. April 4-6, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Vuong C. N., Graham B. D., Coles M. E., Graham, L. E., Teague K. D., Tellez G., Sellers H. S., Garritty C., Hargis, B. M. Current status of avian adenovirus control in the south-central US: hemorrhagic enteritis and inclusion body hepatitis. International Conference of Poultry Intestinal Health. Rome, Italy. April 4-6, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Tellez G., Maguey J., Michel M., Latorre J. D., Arreguin-Nava M., Vuong C., Hargis B. M., Tellez G. Effect of Bacillus-DFM (NorumTM) on leaky gut, bone mineralization, serum peoptide YY concentration and ammonia excretion in turkey poults fed with a rye-based diet. Poultry Science Association Annual Meeting. Montreal, Canada. July 15-18, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Ruff, J., Blankenship J., Lester, H., Selby C. M., Graham B. D., Vuong C. N., Dridi S., Greene E., Hargis B. M., Tellez G. Evaluation of heat stress as a model to induce gastrointestinal leakage in broiler chickens. Poultry Science Association Annual Meeting. Montreal, Canada. July 15-18, 2019
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Selby, C. M., Graham, B. D., Teague, K. D., Graham, L. E., Vuong C. N., Tellez, G., Hargis B. M. Development of an Escherichia coli spray challenge model for neonatal broiler chickens. Poultry Science Association Annual Meeting. Montreal, Canada. July 15-18, 2019


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Presentations and commercial transfer to poultry producers, poultry veterinarians, and poultry scientists were made many times this last year, at State, National and International forums. Local visits by veterinarians, producers and scientists to our facilities and vice versa. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project assisted in the graduation of 3 PhD students and one MS student during the last year, and has provided opportunities for one postdoctoral associate and one scientist from soft funding sources. Moreover, several undergraduate students have been exposed to our area of agricultural research, some may continue their studies following graduation. How have the results been disseminated to communities of interest?Journal articles, book chapters, presentations with abstracts and invited presentations internationally are listed. A large number of patents were issued during the last year, and much of this technology has been licensed from the UA Division of Agriculture. Some technologies have been recently adopted by US poultry companies. Numerous face to face meetings with industry leaders. What do you plan to do during the next reporting period to accomplish the goals?We will continue to work toward sustainable non-antibiotic solutions to ongoing and emerging poultry health issues.

Impacts
What was accomplished under these goals? Substantial progress toward these goals is documented in the journal articles, book chapters, presentations with abstracts and invited presentations internationally. A large number of patents were issued during the last year, and much of this technology has been licensed from the UA Division of Agriculture. Some technologies have been recently adopted by US poultry companies.

Publications

  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Daniel Hernandez-Patlan, Bruno Solis-Cruz, Billy Hargis and Guillermo T�llez. 2018. Applied Research of Chitosan to Control Food Borne Pathogens and Mycotoxins in Poultry. In "Chitin-Chitosan Myriad Functionalities Applications in Science and Technology", Dr. Rajendra Dongre (Ed.), Publisher: IntechOpen, 2018, ISBN 978-953-51-6001-4. DOI: 10.5772/intechopen.76041
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Mikayla F. A. Baxter, Billy M. Hargis and Guillermo Tellez-Isaias. 2018. Assesment of a Nutritional Rehabilitation Model in two modern broilers and their Jungle Fowl ancestor. In Application of Genetics and Genomics in Poultry Science, Prof. Xiaojun Liu (Ed.), Publisher: IntechOpen, 2018, ISBN 978-953-51-6001-6. DOI: 10.5772/intechopen.77090
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Adhikari, B., Y. M. Kwon, B. Hargis, and G. T�llez. 2018. Prokaryotes rule the world. Gut Microbiota-Brain Axis. IntechOpen.
  • Type: Book Chapters Status: Published Year Published: 2018 Citation: Solis-Cruz, B., D. Hernandez-Patlan, B. Hargis, and G. T�llez. 2018. Control of Aflatoxicosis in Poultry Using Probiotics and Polymers. Fungi and Mycotoxins - Their Occurrence, Impact on Health and the Economy as well as Pre- and Postharvest Management Strategies. IntechOpen.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yang Y., J. D. Latorre, B. Khatri, Y. M. Kwon, B. W. Kong, K. D. Teague, L. E. Graham, A. D. Wolfenden, B. D. Mahaffey, M. Baxter, X. Hernandez-Velasco, R. Merino-Guzman, B. M. Hargis, and G. Tellez. 2018. Characterization and evaluation of lactic acid bacteria candidates for intestinal epithelial permeability reduction and Salmonella Typhimurium colonization in neonatal turkey poults. Poultry Science. 97:515521 http://dx.doi.org/10.3382/ps/pex311
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: V. Jazi, A. Ashayerizadeh, M. Toghyani, A. Shabani, G. Tellez, and M. Toghyani. 2018. Fermented soybean meal exhibits probiotic properties when included in Japanese quail diet in replacement of soybean meal. Poultry Science. 0:110 http://dx.doi.org/10.3382/ps/pey071
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Maguey-Gonzalez, J.A., Michel, M.A., Baxter, M.F., Tellez Jr, G., Moore Jr, P.A., Solis-Cruz, B., Hern�ndez-Patlan, D., Merino-Guzman, R., Hernandez-Velasco, X., Latorre, J.D. and Hargis, B.M., 2018. Effect of humic acids on intestinal viscosity, leaky gut and ammonia excretion in a 24 hr feed restriction model to induce intestinal permeability in broiler chickens. Animal Science Journal: 1-9 DOI: 10.1111/asj.13011
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Jesus A. Maguey-Gonzalez, Matias A. Michel, Mikayla F.A. Baxter, Bruno Solis-Cruz, Daniel Hernandez-Patlan, Ruben Merino-Guzman, Xochitl Hernandez-Velasco, Juan D. Latorre, Billy M. Hargis, Guillermo Tellez, Sergio Gomez-Rosales. 2018. Effects of humic acids on recovery of Salmonella enterica serovar Enteritidis. Annals An Sc.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hernandez-Patlan, D., Solis-Cruz, B., M�ndez-Albores, A., Latorre, J.D., Hernandez-Velasco, X., Tellez, G. and L�pez-Arellano, R. 2018. Comparison of PrestoBlue� and plating method to evaluate antimicrobial activity of ascorbic acid, boric acid and curcumin in an in vitro gastrointestinal model. Journal of applied microbiology, 124(2), pp.423-430.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Mikayla F.A. Baxter, Juan D Latorre, Dawn A. Koltes, Sami Dridi, Elizabeth S Greene, Stephen W. Bickler, Jae H. Kim, Ruben Merino-Guzman, Xochitl Hernandez-Velasco, Nicholas B. Anthony, Walter Gay Bottje, Billy M. Hargis, Guillermo Tellez. 2018. Assessment of a Nutritional Rehabilitation Model in two modern broilers and their Jungle Fowl ancestor: A model for better understanding childhood undernutrition. Frontiers in Nutrition.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: L.C. Beer, L.R. Bielke, J.R. Barta, O.B. Faulkner, J.D. Latorre, W.N. Briggs, K. M. Wilson, M. F. A., Baxter, G. Tellez, and B.M. Hargis. 2018. Evaluation of autofluorescent Eimeria maxima oocysts as a potential indicator of non-viability when enumerating oocysts. Poultry Science 97: 2684-2689. Http://dx.doi.org/10.3382/ps/pey124
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yichao Yang, Guillermo Tellez, Juan D Latorre, Pamela M Ray, Xochitl Hernandez, Billy M Hargis, Steven C. Ricke, Young Min Kwon. 2018. Salmonella excludes Salmonella in poultry: Confirming an old paradigm using conventional and barcode-tagging approaches. Frontiers in Veterinary Science. 5:101 doi: 10.3389/fvets.2018.00101
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Adhikari B, Kwon YM, Hargis BM and Tellez G. 2018. How Trillions of Microbes Residing on Gastrointestinal Tract Maintain Homeostasis with Host Cells? Food Nutr J: FDNJ-170. DOI: 10.29011/2575-7091. 100070
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Patl�n, D., SOLIS, B., Pontin, K.P., Latorre, J.D., Baxter, M.F., Hernandez-Velasco, X., Merino-Guzman, R., M�ndez-Albores, A., Hargis, B.M., L�pez-Arellano, R. and Tellez, G., 2018. Evaluation of a solid dispersion of curcumin with polyvinylpyrrolidone and boric acid against Salmonella Enteritidis infection and intestinal permeability in broiler chickens: a pilot study. Frontiers in Microbiology, 9, p.1289.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hern�ndez-Mel�ndez D., Salas-T�llez E., Zavala-Franco A., T�llez G., M�ndez-Albores A., V�zquez-Dur�n A. 2018. Inhibitory effect of flower-shaped zinc oxide nanostructures on the growth and aflatoxin production of a highly toxigenic strain of Aspergillus flavus Link. Materials
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Hargis, B.M. 2018. Invited Presentation: Manipulation and optimization of gut health without antibiotics for poultry. Tri-State Poultry Meeting, Indianapolis, May 22-23.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Hargis, B.M. 2018. Emerging protozoal issues within US turkey production. Hafez Turkey Conference, Berlin, June 31.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Hargis, B.M. 2018. Invited Presentation: Coccidiosis Mitigation With Declining Efficacy of Ionophores, Chemicals, and Vaccination Strategies. Poultry Universe Conference, Curitibita, Brazil, August 13-15.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Hargis, B.M. 2018. Invited Presentation: Selection and Applications of Direct Fed Microbials for Commercial Poultry. JBS Poultry, Sao Paulo, August 16.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Juan Latorre*, Daniel Hernandez-Patl�n, Bruno Solis-Cruz, Karine Pontin, Lisa Bielke, Billy Hargis, Guillermo Tellez. Effect of the inclusion of a Bacillus Direct-fed microbial on growth performance, lesion score and intestinal barrier integrity in broiler chickens using a necrotic enteritis model. International Poultry cientific Forum January 29-30, 2018. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Juan Latorre*, Daniel Hernandez-Patl�n, Bruno Solis-Cruz, Karine Pontin, Lisa Bielke, Billy Hargis, Guillermo Tellez.Effects of the combination of curcumin and polyvinylpyrrolidone with or without boric acid on Salmonella Enteritidis colonization and gut health parametersin broilers chickens. Poultry Science Association 2018 Annual Meeting. San Antonio, Texas.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Mikayla Baxter*GS, Juan David Latorre, Si Hong Park, Steve Ricke, Sun Ae Kim, Billy Hargis, Guillermo Tellez-Isaias. Evaluating microbial populations in three sections of the left and right ceca from broiler chickens fed two different diets. Poultry Science Association 2018 Annual Meeting. San Antonio, Texas.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Hargis, B.M. 2018. Invited Presentation: Practical Implementation of Salmonella Control Measures Within and Between Commercial Broiler Breeder and Production Flocks. CPF- Thailand, February 22nd, 2018.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Hargis, B.M. 2018. Opening Welcome and Program Introduction at Poultry Science Association LATAM meeting  and Closing Remarks. Campinas, Sao Paulo, Brazil. November 6-8.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Daniel Hernandez-Patlan, Bruno Solis-Cruz, Mario Alberto Cano-Vega, Eric Beyssac, Ghislain Garrait, Xochitl Hernandez-Velasco, Raquel Lopez-Arellano, Guillermo Tellez, Gustavo R. Rivera-Rodriguez. 2018. Development of chitosan and alginate nanocapsules to increase the solubility, permeability and stability of curcumin. Journal of Pharmaceutical Innovation. August 14. https://doi.org/10.1007/s12247-018-9341-1
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Graham, L.E., Teague, K.D., Latorre, J.D., Yang, Y., Baxter, M.F.A., Mahaffey, B.D., Hernandez-Velasco, X., Bielke, L.R., Hargis, B.M. and Tellez, G. 2018. Use of probiotics as an alternative to formaldehyde fumigation in commercial broiler chicken hatch cabinets. The Journal of Applied Poultry Research. 27: 371-379. http://dx.doi.org/10.3382/japr/pfy008
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Anai Zavala-Franco, Daniel Hern�ndez-Patl�n, Bruno Sol�s-Cruz, Raquel L�pez-Arellano, Guillermo Tellez-Isaias, Alma V�zquez-Dur�n, Abraham M�ndez-Albores. 2018. Assessing the Aflatoxin B1 Adsorption Capacity between Biosorbents using an in vitro Multi-Compartmental Model Simulating the Dynamic Conditions in the Gastrointestinal Tract of Poultry. Toxins. 10, 484; doi:10.3390/toxins10110484
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Raehtz, S., Hargis, B.M., Kuttappan, V.A., Pamukcu, R., Bielke, L.R. and McCabe, L.R., 2018. High molecular weight polymer promotes bone health and prevents bone loss under Salmonella challenge in broiler chickens. Frontiers in physiology, 9, p.384.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Wang, Hong, Juan David Latorre Cardenas, Mohit Bansal, Bilal Al-Rubaye, Guillermo Tellez, Billy Hargis, and Xiaolun Sun. 2018. Microbiota metabolic product deoxycholic acid controls chicken necrotic enteritis. bioRxiv: 215640.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Mikayla F. A. Baxter1, Sami Dridi1, Dawn A. Koltes1,2, Juan D. Latorre1, Walter G. Bottje1, Elizabeth S. Greene1, Stephen W. Bickler3, Jae H. Kim4, Ruben Merino-Guzman5, Xochitl Hernandez-Velasco5, Nicholas B. Anthony1, Billy M. Hargis1, and Guillermo Tellez-Isaias. 2018. Assessment of a Nutritional Rehabilitation Model in two Modern Broilers and their Jungle Fowl Ancestor: Evaluation of Gut Barrier Function. Frontiers in Genetics.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Mikayla Baxter*GS, Juan David Latorre, Si Hong Park, Steve Ricke, Sun Ae Kim, Billy Hargis, Guillermo Tellez-Isaias. Evaluating the effect of rye versus corn as a source of energy on the microbiome in broiler chickens in a nutritional rehabilitation model. International Poultry Scientific Forum January 29-30, 2018. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Bishnu Adhikari*GS, Guillermo Tellez-Isaias, Kyle Teague, Young Kwon. Are chicken embryos sterile? An investigation through both culture-dependent and independent methods. International Poultry Scientific Forum January 29-30, 2018. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Brittany Graham*GS, Kabel Robbins, Lucas Graham, Kyle Teague, Amanda Wolfenden, Guillermo Tellez, Billy Hargis. Evaluation of the effects of a Turkey Cellulitis/Dermatitis oil emulsion vaccine on immune response and mortality under commercial conditions. International Poultry Scientific Forum January 29-30, 2018. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Paula Johnson*GS, Jacob Lum, Lucas Graham, Kyle Teague, Brittany Mahaffey-Graham, Mikayla Baxter, Juan David Latorre, Cheryl Lester, Guillermo Tellez-Isaias, Karen Christensen, Billy Hargis.Evaluation of the effect of formaldehyde or heat stress on body weights and body weight gain in neonatal broiler chickens. International Poultry Scientific Forum January 29-30, 2018. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Lemuel Reber, Mikayla Baxter, Billy Hargis, Guillermo Tellez*, Dawn Koltes. Effects of a malabsorptive rye diet on growth and adipose tissue in commercial broilers. International Poultry Scientific Forum January 29-30, 2018. Georgia World Congress Center. Atlanta, Georgia.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Juan D. Latorre, Bishnu Adhikari, Si Hong Park, Kyle D. Teague, Lucas E. Graham, Brittany D. Mahaffey, Mikayla F.A. Baxter, Xochitl Hernandez, Young Min Kwon, Steven C. Ricke, Lisa Bielke, Billy M Hargis, Guillermo Tellez. 2018. Evaluation of the epithelial barrier function and ileal microbiome in an established necrotic enteritis challenge model in broiler chickens. Frontiers in Veterinary Science. 5, p.199. doi: 10.3389/fvets.2018.00199


Progress 12/13/16 to 09/30/17

Outputs
Target Audience:Poultry scientists, poultry industry, vaccine companies, probiotics companies Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Eight graduate students and three postdoctoral associates are involved in this project. How have the results been disseminated to communities of interest?Refereed publications, regional and national meetings, and individual meetings with numerous members of the U.S. poultry industry. What do you plan to do during the next reporting period to accomplish the goals?Progress is coming very quickly on this project. We anticipate several invention disclosures and approximately 12 refereed publications during the next year.

Impacts
What was accomplished under these goals? Several projects involving pre-natal or neonatal exposure of chicks to beneficial microflora were published, and intellectual property protection is being sought (Patents Pending). Two of these projects are under commercial development by multinational companies for use in broiler hatcheries, and turkey hatchery application is being investigated. Further progress of recombinant vaccine development was also reported in the citations. Major impacts include development of models for induction and measurement of leaky gut (including bacterial translocation) were completed. These models are currently being used for efforts to ameloriate the impact of enteric stressors on improving gut barrier function in broilers and turkeys.

Publications

  • Type: Book Chapters Status: Published Year Published: 2017 Citation: B. M. Hargis, G. Tellez, L.R. Bielke. 2017. Gut health and susceptibility to enteric bacterial diseases in poultry. In: Achieving sustainable production of poultry meat
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kuttappan, V.A., Owens, C.M., Coon, C., Hargis, B.M. and Vazquez-A�on, M., 2017. Incidence of broiler breast myopathies at 2 different ages and its impact on selected raw meat quality parameters. Poultry Science, p.pex072.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Vuong, C.N., Chou, W.K., Kuttappan, V.A., Hargis, B.M., Bielke, L.R. and Berghman, L.R., 2017. A Fast and Inexpensive Protocol for Empirical Verification of Neutralizing Epitopes in Microbial Toxins and Enzymes. Frontiers in Veterinary Science, 4, p.91
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kuttappan, V.A., Bottje, W., Ramnathan, R., Hartson, S.D., Coon, C.N., Kong, B.W., Owens, C.M., Vazquez-A�on, M. and Hargis, B.M., 2017. Proteomic analysis reveals changes in carbohydrate and protein metabolism associated with broiler breast myopathy. Poultry Science.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Abousaad, S., Lassiter, K., Piekarski, A., Chary, P., Striplin, K., Christensen, K., Bielke, L.R., Hargis, B.M., Dridi, S. and Bottje, W.G., 2017. Effect of in ovo feeding of dextrin-iodinated casein in broilers: II. Hatch window and growth performance. Poultry science, 96(5), pp.1478-1484.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Flees, J., Sharifabadi, H.R., Greene, E., Beer, L., Hargis, B.M., Ellestad, L., Porter, T., Donoghue, A., Bottje, W.G. and Dridi, S., 2017. Effect of Morinda citrifolia (Noni)-enriched diet on hepatic heat shock protein and lipid metabolism-related genes in heat stressed broiler chickens. Frontiers in Physiology, 8, p.919.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Bielke, L.R., Hargis, B.M. and Latorre, J.D., 2017. Impact of Enteric Health and Mucosal Permeability on Skeletal Health and Lameness in Poultry. In Understanding the Gut-Bone Signaling Axis (pp. 185-197). Springer, Cham
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Omar F. Prado-Rebolledo, Jaime de Jesus Delgado-Machuca, Rafael J. Macedo-Barragan, Luis J. Garcia-M�rquez, Jesus E. Morales-Barrera, Juan D. Latorre, Xochitl Hernandez-Velasco, and Guillermo Tellez. 2017. Evaluation of a selected lactic acid bacteria-based probiotic on Salmonella Enteritidis colonization and intestinal permeability in broiler chickens. 46, 1: 90-94. Avian Pathology.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Michel MA, Revidatti FA, Fern�ndez RJ, Sindik ML, Sanz P, Xochitl Hernandez-Velasco, Juan D Latorre, Billy M Hargis, and Guillermo Tellez. 2017. Evaluation of a Commercially Available Probiotic and Organic Acid Blend Product on Production Parameters and Economics in Broiler Breeders. Nutr Food Technol Open Access 3(1): doi http://dx.doi. org/10.16966/2470-6086.139
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: J. D. Latorre, X. Hernandez-Velasco, J. L. Vicente, R. Wolfenden, B. M. Hargis, and Guillermo Tellez. 2017. Effects of the inclusion of a Bacillus direct-fed microbial on performance parameters, bone quality, recovered gut microflora, and intestinal morphology in broilers consuming a grower diet containing corn distillers dried grain with solubles. Poultry Science. 0:18 http://dx.doi.org/10.3382/ps/pex082
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: K. D. Teague, L. E. Graham, J. R. Dunn, H. H. Cheng, N. Anthony, J. D. Latorre, A. Menconi, R. E. Wolfenden, A. D. Wolfenden, B. D. Mahaffey, M. Baxter, X. Hernandez-Velasco, R. Merino-Guzman, L. R. Bielke, B. M. Hargis, and G. Tellez. 2017. In ovo evaluation of FloraMax�-B11 on Marek�s disease HVT vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella Enteritidis infection in broiler chickens. Poultry Science. 00:19 http://dx.doi.org/10.3382/ps/pew494
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Yichao Yang, Steven C. Ricke, Guillermo Tellez, Young Min Kwon 2017. Quantitative Tracking of Salmonella Enteritidis Transmission Routes Using Barcode-tagged Isogenic Strains in chickens: Proof of Concept Study. Frontiers in Veterinary Science. 4:15. doi: 10.3389/fvets.2017.00015
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Baxter, M.F., Merino-Guzman, R., Latorre, J.D., Mahaffey, B.D., Yang, Y., Teague, K.D., Graham, L.E., Wolfenden, A.D., Hernandez-Velasco, X., Bielke, L.R. and Hargis, B.M., 2017. Optimizing Fluorescein Isothiocyanate Dextran Measurement As a Biomarker in a 24-h Feed Restriction Model to Induce Gut Permeability in Broiler Chickens. Frontiers in Veterinary Science, 4: 56
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Juxing Chen, Guillermo Tellez, Jeffery Escobar, & Mercedes Vazquez-Anon. Impact of Trace Minerals on Wound Healing of Footpad Dermatitis in Broilers. 2017. Scientific Reports. Nature 7: 1894. DOI:10.1038/s41598-017-02026-2
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Prado-Rebolledo, O.F., Molina-Ochoa, J., Lezama-Guti�rrez, R., Garc�a-M�rquez, L.J., Minchaca-Llerenas, Y.B., Morales-Barrera, E., Tellez, G., Hargis, B., Skoda, S.R. and Foster, J.E., 2017. Effect of Metarhizium anisopliae (Ascomycete), Cypermethrin, and D-Limonene, Alone and Combined, on Larval Mortality of Rhipicephalus sanguineus (Acari: Ixodidae). Journal of medical entomology. May 4.