BREEDING COMMON BEAN (PHASEOLUS VULGARIS L.) FOR RESISTANCE TO ABIOTIC AND BIOTIC STRESSES, SUSTAINABLE PRODUCTION, AND ENHANCED NUTRITIONAL

• Sponsoring Institution: National Institute of Food and Agriculture
• Project Status: COMPLETE
• Funding Source: HATCH
• Reporting Frequency: Annual
• Accession No.: 1011620
• Multistate No.: W-3150
• Project Start Date: Oct 1, 2015
• Project End Date: Feb 26, 2020
• Program Code: [(N/A)]- (N/A)

Recipient Organization
UNIVERSITY OF GEORGIA
200 D.W. BROOKS DR
ATHENS,GA 30602-5016

Performing Department
Crop & Soil Sciences

Non Technical Summary
We are using modern genetic tools to find genes that were selected for during adapatation of bean from South America to Europe in the hope that this information can be used to design beans for the future.

Animal Health Component

25%

Research Effort Categories

Basic

75%

Applied

25%

Developmental

(N/A)

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
201	1410	1080	100%

Knowledge Area
201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1410 - Beans (dry);

Field Of Science
1080 - Genetics;

Keywords

bean

germ plasm

genomics

adaptation

Goals / Objectives
Improving bean yield potential by incorporating resistance/tolerance to major biotic and abiotic stresses, broadening the genetic base, implementing/integrating genomic resources and coordinating field trial nurseries. Analyze, document, and utilize genomic resources to enhance nutritional qualities and identify diversity within Phaseolus vulgaris to facilitate development of nutritious food products to promote human health and well-being. The overall strategy is based on collaborative research of constraints shared across production regions. This collaboration includes germplasm and pathogen exchange, sharing of protocols and techniques (e.g. DNA markers, virus isolates and infectious viral clones, field/greenhouse/lab. screening methodologies, etc.), regional nurseries and trials, and screening of genotypes for the traits of interest. As a result of this exchange of knowledge and material, breeding projects will be able to introgress and pyramid favorable alleles and QTL for enhanced seed yield potential, nutritional value, and resistance to multiple abiotic and biotic stresses using a multi-disciplinary and multi-institutional team approach. To accomplish these objectives, our research activities are divided into various sub-projects (see Appendix 1) in which researchers from two or more participating states and institutions conduct research on each major problem as a team. To identify and set priorities, all W-3150 participating researchers and stakeholders (such as growers and industry), will be periodically consulted about production problems and deficiencies in the available germplasm.

Project Methods
Genotyping by sequencing of 10,000 accessions. RNA seq and whole genome resequencing on a subset of accessions.

Progress 10/01/15 to 02/26/20

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Contributed to work on disease resistance introgression in peanut.

Publications

Progress 10/01/16 to 09/30/17

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We continue work on developing genomic resources for common bean and its germplasm. More than 100 lines have been used to produce DNA methylomes, small RNA and RNA-seq datasets.

Publications

Progress 10/01/15 to 09/30/16

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We have genotyped ~2,000 genebank accessions and are in the process of making this data public as a tool to interrogate genebank collections from a genetic perspective.

Publications

• Type: Journal Articles Status: Published Year Published: 2017 Citation: Richard, M.M.S., A. Gratias, V. Thareau, K-D. Kim, S. Balzergue, J. Joets, S.A. Jackson and V. Geffroy. 2017. Genomic and epigenomic immunity in common bean: the unusual features of NB-LRR gene family. Nucleic Acid Res. doi.org/10.1093/dnares/dsx046.
• Type: Journal Articles Status: Published Year Published: 2016 Citation: Bitocchi, E., D. Rau, A. Benazzo, E. Bellucci, D. Goretti, E. Biagetti, A. Panziera, G. Laid�, M. Rodriguez, G. Attene, P. McClean, R. Lee, S.A. Jackson, G. Bertorelle, R. Papa. 2016. High Level of Non-Synonymous Changes in Common Bean Suggests that Selection under Domestication Increased Functional Diversity at Target Traits. Frontiers in Plant Sci. DOI: 10.3389/fpls.2016.02005.
• Type: Journal Articles Status: Published Year Published: 2016 Citation: Iwata-Otsubo, Al, B. Radke, S. Findley, B. Abernathy, C. E. Vallejos, and S.A. Jackson. 2016. Fluorescence in situ hybridization (FISH)-based karyotyping reveals rapid evolution of centromeric and subtelomeric repeats in common bean (Phaseolus vulgaris) and relatives. G3. doi:10.1534/g3.115.024984.
• Type: Journal Articles Status: Published Year Published: 2016 Citation: Gao, G., D. Zhao,B. Abernathy, A. Iwata, Y. Li, A. Herrera-Estrella, N. Jiang and S.A. Jackson. 2016. Dynamics of a novel highly repetitive CACTA family in common bean (Phaseolus vulgaris). G3. doi:10.1534/g3.116.028761.