Source: TEXAS A&M UNIVERSITY submitted to
NANOTECHNOLOGY APPLICATIONS IN BIOLOGICAL, AGRICULTURAL, AND FOOD MATERIALS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
NEW
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1011441
Grant No.
(N/A)
Project No.
TEX0-1-9464
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 31, 2016
Project End Date
Oct 7, 2021
Grant Year
(N/A)
Project Director
Gomes, C.
Recipient Organization
TEXAS A&M UNIVERSITY
750 AGRONOMY RD STE 2701
COLLEGE STATION,TX 77843-0001
Performing Department
Biological & Agricultural Engineering
Non Technical Summary
This research program is aimed at using nanotechnology approaches to engineer and design materials to be applied to agricultural and biological systems. This project is divided into two specific areas of study: (1) applications of nanotechnology to monitor and enhance quality characteristics in biological and agricultural materials, and (2) applications of nanotechnology to monitor and enhance biological and agricultural materials safety.Research is necessary to better understand the structural design principles for delivery systems, sensor platforms, and thin film polymers at the nanoscale level in order to achieve specific functional and sensing performances in food and agricultural materials. The information obtained from this research program will be extremely useful for the food and agricultural industries as it enables manufacturers to improve the properties of existing products, optimize processing conditions, develop new products, monitor product integrity and quality, and reduce manufacturing costs. There is a need for research to better understand the structural design principles for delivery systems, thin film polymers, and sensing platforms at the nanoscale level in order to achieve specific functional and sensing performances to monitor and ensure safe food and agricultural material supplies.It will also help in understanding the relationship among the nanoscopic, microscopic, and macroscopic features of agricultural and food materials, and the bulk physicochemical properties, sensory attributes, and the physiological performance of agricultural materials.The proposed project will provide a research intensivelearning environment for students in science and engineering in the area of biopolymers and coatings, bioactivematerials, processing and physicochemical properties of materials, materials characterization, and food safety
Animal Health Component
0%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5015010202012%
5025010202012%
5035010202012%
7125010202014%
7115010202014%
5115010202012%
7015010202012%
7235010202012%
Knowledge Area
511 - New and Improved Non-Food Products and Processes; 711 - Ensure Food Products Free of Harmful Chemicals, Including Residues from Agricultural and Other Sources; 501 - New and Improved Food Processing Technologies; 712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins; 503 - Quality Maintenance in Storing and Marketing Food Products; 723 - Hazards to Human Health and Safety; 502 - New and Improved Food Products; 701 - Nutrient Composition of Food;

Subject Of Investigation
5010 - Food;

Field Of Science
2020 - Engineering;
Goals / Objectives
(1) Applications of nanotechnology to monitor and enhance quality characteristics in biological and agricultural materials.The goal of this research is to expand current knowledge in the area of nanotechnology as it applies to agricultural and food systems for new and improved utilization. Specific objectives of this research are to: (1) design delivery systems to carry, protect, and control the release of functional compounds to their specific site of action (i.e., increasing their bioavailability), (2) develop sensing platforms to monitor food quality (i.e., freshness) and authenticity in real-time as food product moves through the supply chain (3) develop thin film polymer coatings for delivery of active ingredients (antioxidants, anti-browning agents, etc.) and for improved performance properties (water vapor and oxygen barriers, thermal stability, etc.). The overall goal of this research is to help enable food producers, manufacturers, and consumers to make broader use of nanotechnology that can add value and improve the quality of existing food and agricultural products.(2) Applications of nanotechnology to monitor and enhance biological and agricultural materials safety.The goal of this research is to design effective antimicrobial interventions technologies for food and agricultural system applications using nanotechnology as a tool. Specific objectives of this research are to: (1) develop and optimize colloidal dispersions that are able to control and extend the release of antimicrobial compounds in food and agricultural systems, (2) develop nano-enabled sensors for real-time monitoring of food contaminants (pathogens, individual biological molecules, etc.), (3) develop thin polymer coatings to be applied on surfaces and food packaging with antimicrobial properties by having tethered antimicrobial agents, bound directly to the surface of the material, or by the release of soluble antimicrobial agents in the surrounding environment. The overall goal of this research is to help enable food producers, manufacturers, and consumers to make broader use of nanotechnology that can ensure and improve safety of food and agricultural products and consequently ensure public health.
Project Methods
The experimental work to be carried out will, whenever possible, follow all the required testing standards and methodologies, such as AOAC (2005) and ASTM (2002). Special attention will also be paid to make sure that the testing approach is scientifically sound. The results of testing and analyses will be documented and published in refereed scientific journals.(1) Applications of nanotechnology to monitor and enhance quality characteristics in biological and agricultural materials.Current efforts in our laboratory include testing of a wide series of active compounds, encapsulating materials, and sensing platforms. Our research plan is to: (1) identify active ingredients (i.e., lipophilic and hydrophilic compounds), encapsulating materials (i.e., phospholipids, biocompatible polymers, etc.), and sensing platforms (polymers, carbon-based materials, and nanometallic structures), (2) study preparative methods, physicochemical characterization, and release properties for each delivery system and thin polymer coating being evaluated, (3) engineer sensing platforms functionalized with polymers and hybrid nanostructures and characterize their performance in detecting the target analyte (i.e., food freshness indicators, biomarkers for authenticity), and (4) determine the effect of using these active ingredient delivery systems (Obj. 2) and the efficiency of sensing platforms for measuring food quality (Obj. 3) on different agricultural and food systems.(2) Applications of nanotechnology to monitor and enhance biological and agricultural materials safety.Current efforts in our laboratory include to (1) select encapsulating materials (i.e., phospholipids, biocompatible polymers, etc.), sensing platforms (polymers, carbon-based materials, and nanometallic structures) and potential antimicrobial agents, i.e., natural compounds including proteins, essential oils, plant extracts, etc.; (2) study preparative methods, physicochemical characterization, , release properties, and antimicrobial efficiency against foodborne pathogens for each delivery system and thin polymer coating being tested; (3) engineer sensing platforms functionalized with polymers and hybrid nanostructures and characterize their performance in detecting food contaminants (pathogens, toxins, etc.); i.e., limit of detection, sensitivity, linear range, response time, etc.; and (4) study the effect of using these antimicrobial systems (Obj. 2) and the sensing platforms efficiency for measuring pathogens and other contaminants (Obj. 3) on different food and agricultural systems.Experimental Design and Data Analysis: For both research objectives empirical/fundamental models or testing methodology will be used as a basis to predict delivery systems, biosensors, and thin polymer coating performance and functionality. Studies on these experiments will provide information on figures of merit for biosensors, i.e., detection limit, sensitivity, linear range, etc., and also the exact amount of functional ingredient or antimicrobial agent and release rate of the active substances required to achieve a given effect under different surrounding environments. Results from these studies will be expanded to other applications such as, disposable sensors, medical devices, drug delivery, among others. A completely randomized design with equal replications will be used in this study. All experiments will be performed in triplicate as independent experiments and results will be expressed as mean ± standard deviation. Data analysis will be performed using JMP v.9 software (SAS Institute, Cary, NC). Differences between variables will be tested for significance by one-way analysis of variance (ANOVA) and significantly different means (p<0.05) will be separated by the Tukey test using JMP v.9 software.Efforts: presentation of scientific work during international conferences, formal classroom instruction, laboratory instruction, workshops and outreach programs.

Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Graduate and undergraduate students, faculty, industry, stake holders, scientific community. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Mentoring of graduate and undergraduate students and visiting scholar were conducted as part of this project. Graduate and undergraduate students were trained on new methodologies for encapsulating natural antimicroibal compounds, microbiology analysis, and physico-chemical analysis of these delivery systems. Furthermore, students and visiting scholars were trained on new methodologies for biosensors design utilizing nano-hybrid materials (graphene and nanoplatinum), nano-metal deposition using sonoelecgrodeposition techniques, stimuli-responsive polymer and aptamer and lectin functionalization for real-time detection of foodborne pathogens. Events: PI presented at International Mechanical Engineering Conference and Exposition. Salt Lake City, Utah, November. Graduate students presented at International Mechanical Engineering Conference and Exposition. Salt Lake City, Utah, November. Graduate students presented at Nano@IAstate, Ames, IA, August. PI presented at American Chemical Society, ACS National Meeting and Expo Fall 2019, San Diego, CA, August. PI presented at SUS-RURI: Developing a Convergence SUS Agenda for Redesigning the Urban-Rural Interface along the Mississippi River Watershed, Ames, IA, August. PI organized and moderated a scientific symposium during 2019 annual IFT meeting on Biosensing technology for practical applications in food production, New Orleans, June. Graduate students and visiting scholar presented at 2019 IFT International Meeting, New Orleans, June. PI presented at Smart Biomedical and Physiological Sensor Technology XV, 2019 SPIE (Baltimore, MA, April). PI presented at American Chemical Society, ACS National Meeting and Expo Spring 2019, Orlando, FL, March. Graduate students and visiting scholars have been trained in biological safety and working with biohazards at Texas A&M University. How have the results been disseminated to communities of interest?Results have been disseminated throughout peer-reviewed publications, PI's website, and presentations at international conferences. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue working toward the goals by conducting experiments, interpreting the data and disseminating the results throghout peer-reviewed publications, PI's website, and presentations at international conferences.

Impacts
What was accomplished under these goals? Developed and optimized of pH-responsive PLGA-chitosan nanoparticles for triggered release of antimicrobials. Developed a flexible stamped multi-layer graphene laminates for disposable in-field electrochemical biosensing. Developed SNAPS: sensor analytics point solutions for detection and decision support systems.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Giacobassi, C. A., Oliveira, D. A., Pola, C. C., Cavallaro, N., McLamore, E. S., Gomes, C. 2019. Real-time monitoring of Escherichia coli spp. for on farm water quality assessment based on temperature-sensitive nanobrush actuation. SUS-RURI: Developing a Convergence SUS Agenda for Redesigning the Urban-Rural Interface along the Mississippi River Watershed, Ames, IA, August.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Martinez-Rosales, A. F., Pola, C., Ramirez-Fuentes, E., Trujillo-Tapia, M. N., Hernandez-Rodrigues, A., Gomes, C. L. 2019. Combined application of microencapsulated biofertilizers containing Fischerella sp. with chemical fertilizers for improved tomato (Solanum Lycopersicum) production. 2019 Institute of Food Technologists (IFT) annual meeting, New Orleans, LA, June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Oliveira, D. A., Stromberg, L. R., Pola, C., Parate, K., Cavallaro, N., Claussen, J. C., McLamore, E. S., Gomes, C. L. 2019. Biomimetic nanosensors for measuring pathogenic bacteria in complex food matrices. Smart Biomedical and Physiological Sensor Technology XV, 2019 SPIE (Baltimore, MA, April), 11020, 110200J
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Rohini, K., Kalia, A., Sharma, S. P., Vikal, Y., Luthra, K., Dheri, G., Gomes, C. L. 2019. Nano-polymer-urea composite for improved fertilizer applications: study of their effect on soil enzymatic activities and microflora dynamics in N-cycle of potato (Solanum tuberosum L.). American Chemical Society, ACS National Meeting and Expo Spring 2019, Orlando, FL, March.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Pola, C. C., Teofilo, R. F., Moraes, A. R. F., Medeiros, A. A., Soares, N. F. F., Gomes, C. 2019. Development and optimization of pH-responsive PLGA-chitosan nanoparticles for triggered release of antimicrobials. Food Chemistry. 295: 671-679.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Stromberg, L., Hondred, J., Sanborn, D., Mendivelso-Perez, D., Ramesh, S., Rivero, I., Kogot, J., Smith, E., Gomes, C., Claussen, J. 2019. Flexible stamped multi-layer graphene laminates for disposable in-field electrochemical biosensing. Microchima Acta. 186 (8), 533
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: McLamore, E. S., Datta, S. P. A., Morgan, V., Cavallaro, N., Kiker, G., Jenkins, D. M., Rong, Y., Gomes, C., Claussen, J., Vanegas, D., Alocilja, E. C. 2019. SNAPS: sensor analytics point solutions for detection and decision support systems. Sensors: 19 (22):4935.
  • Type: Book Chapters Status: Under Review Year Published: 2019 Citation: Nayak, P., Stromberg, L., Hondred, J., Garland, N., Hall, L., Parate, K., Chen, B., Gomes, C., Claussen, J. 2019. Materials and manufacturing of wearable bioelectronic devices. In: Wearable Bioelectronics. Eds: Parlak, O., Salleo, A., Turner, A. P. F.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Pola, C. C., Hjort, R., Parate, K., Claussen, J. C., McLamore, E. S., Gomes, C. 2019. Flexible laser-induced graphene for pathogen sensing in food and agricultural settings. ASME 2019 IMECE. International Mechanical Engineering Conference and Exposition. Salt Lake City, Utah, November.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Pola, C. C., Parate, K., Claussen, J. C., Gomes, C. 2019. Laser-induced graphene interdigitated electrodes for rapid detection of Salmonella enterica in food samples. ASME 2019 IMECE. International Mechanical Engineering Conference and Exposition. Salt Lake City, Utah, November
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Parate, K., Pola, C. C., Rangekar, S. V., Mendivelso-Perez, D. L., Smith, E. A., Hersam, M. C., Gomes, C. L., Claussen, J. C. 2019. Aerosol jet printed graphene array-based immunosensor for fish spoilage monitoring. ASME 2019 IMECE. International Mechanical Engineering Conference and Exposition. Salt Lake City, Utah, November.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Pola, C. C., Parate, K., Claussen, J., Gomes, C. 2019. Laser induced graphene for foodborne pathogen detection. Nano@IAstate, Ames, IA, August.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Giacobassi, C., Oliveira, D., Pola, C., Cavallaro, McLamore, E., Gomes, C. L. 2019. Rapid, label-free detection of Escherichia coli spp. for on-farm water quality assessment based on temperature-sensitive nanobrush actuation. American Chemical Society, ACS National Meeting and Expo Fall 2019, San Diego, CA, August.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Hondred, J., Garland, N., Kucherenko, I., Hjort, R., Gomes, C. L., Claussen, J. 2019. Printed and laser induced graphene electrochemical sensors for in-field pesticide and fertilizer ion monitoring. American Chemical Society, ACS National Meeting and Expo Fall 2019, San Diego, CA, August.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Cavallaro, N., Gomes, C. L., McLamore, E. S. 2019. Laser scribed graphene sensors for point of use detection of Listeria monocytogenes. American Chemical Society, ACS National Meeting and Expo Fall 2019, San Diego, CA, August.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Graduate and undergraduate students, faculty, industry, stake holders, and scientific community. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Mentoring of graduate and undergraduate students and visiting scholar were conducted as part of this project. Graduate and undergraduate students were trained on new methodologies for encapsulating natural antimicroibal compounds, microbiology analysis, and physico-chemical analysis of these delivery systems. Furthermore, students and visiting scholars were trained on new methodologies for biosensors design utilizing nano-hybrid materials (graphene and nanoplatinum), nano-metal deposition using sonoelecgrodeposition techniques, stimuli-responsive polymer and aptamer and lectin functionalization for real-time detection of foodborne pathogens. Events: PI presented at American Chemical Society (ACS) Midwest Regional Meeting, Ames, IA, October. PI presented at QEEN II: 2nd Quantifying Exposure to Engineered Nanomaterials from Manufactured Products Workshop, Washington, DC, October. PI organized session organized and moderated a scientific symposium during 2018 Conference of Food Engineering (CoFE), Minneapolis, MN, September on biosensors Challenges and Opportunities for Commercial PI presented at 2018 Conference of Food Engineering (CoFE), Minneapolis, MN, September. Graduate students and visiting scholars presented at Conference of Food Engineering (CoFE), Minneapolis, MN, September. PI attended 2018 Institute of Food Technologists (IFT) annual meeting, Chicago, IL, July. PI organized and moderated a scientific symposium during 2018 annual IFT meeting on Challenges and Opportunities for Commercial Biosensors, Chicago, IL, July. Graduate students and visiting scholar presented at 2018 IFT International Meeting, Chicago, IL, July. PI, graduate students and visiting scholar presented at World Congress on Biosensors 2018, Miami, FL, June. PI presented at Nanoscale Science and Engineering for Agriculture and Food Systems - Gordon Research Conference - South Hadley, MA, June. Post-doc student presented at 2018 Iowa State Research Day, Ames, IA, April. Graduate student presented at 2018 Institute of Biological Engineering (IBE), Norfolk, VA, April. Graduate students and visiting scholars have been trained in biological safety and working with biohazards at Texas A&M University. How have the results been disseminated to communities of interest?Results have been disseminated throughout peer-reviewed publications, PI's website, and presentations at international conferences. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue working toward the goals by conducting experiments, interpreting the data and disseminating the results throughout peer-reviewed publications, PI's website, and presentations at international conferences.

Impacts
What was accomplished under these goals? Developed nanoparticles composed of Lanthanide ions doped in vanadium oxide for sensitive optical glucose detection. Reviewed fluorescent nanodiamonds for luminescent thermometry in the biological transparency window. Develop a method consisted of Tip-enhanced Raman scattering of DNA aptamers for Listeria monocytogenes detection. Developed Laser scribed graphene biosensor for detection of biogenic amines in food samples using locally sourced materials. Developed post hoc support vector machine learning for impedimetric biosensors based on weak protein-ligand interactions. Developed a method of actuation of chitosan-aptamer nanobrush borders for pathogen sensing. Developed a preparation of black pepper oleoresin inclusion complexes based on beta-cyclodextrin for antioxidant and antimicrobial delivery applications using kneading and freeze drying methods: a comparative study. Studied the effect of nanoencapsulation using PLGA on antioxidant and antimicrobial activities of guabiroba fruit phenolic extract. Reviewed bacteria sensing in food processing facilities. Developed a selective separation method of proteins using stimuli-responsive polymers in an aqueous system

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Talib, A. J., Alkahtani, M., Jiang, L., Alghannam, F., Brick, R., Gomes, C. L., Scully, M. O., Sokolov, A. V., Hemmer, P. R. 2018. Lanthanide ions doped in vanadium oxide for sensitive optical glucose detection. Optical Materials Express. 8 (11):3277-3287.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Alkahtani, M. H., Alghannam, F., Jiang, L., Rampersaud, A. A., Brick, R., Gomes, C. L., Scully, M. O., Hemmer, P. R. 2018. Fluorescent nanodiamanods for luminescent thermometry in the biological transparency window. Optics Letters. 43 (14): 3317-3320.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: He, S., Li, H., Gomes, C. L., Voronine, D. V. 2018. Tip-enhanced Raman scattering of DNA aptamers for Listeria monocytogenes. Biointerphases. 13 (3):03C402.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Vanegas, D. C., Patino, L., Mendez, C., Oliveira, D. A., Torres, A. M., Gomes, C. L., McLamore, E. S. 2018. Laser scribed graphene biosensor for detection of biogenic amines in food samples using locally sourced materials. Biosensors. 8 (2).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Rong, Y., Padron, A. V., Hagerty, K. J., Nelson, N., Chi, S., Keyhani, N. O., Katz, J., Datta, S. P. A., Gomes, C. L., McLamore, E. S. 2018. Post hoc support vector machine learning for impedimetric biosensors based on weak protein-ligand interactions. Analyst. 143 (9): 2066-2075.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hills, K. D., Oliveira, D. A., Cavallaro, N. D., Gomes, C. L., McLamore, E. S. 2018. Actuation of chitosan-aptamer nanobrush borders for pathogen sensing. Analyst. 143 (7): 1650-1661.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Ozdemir, N., Pola, C. C., Teixeira, B. N., Hill, L. E., Bayrak, A., Gomes, C. 2018. Preparation of black pepper oleoresin inclusion complexes based on beta-cyclodextrin for antioxidant and antimicrobial delivery applications using kneading and freeze drying methods: a comparative study. LWT-Food Science. 91: 439-445.
  • Type: Book Chapters Status: Under Review Year Published: 2018 Citation: Oliveira, D. A., Stromberg, L., Claussen, J. C., Gomes, C. 2017. Bacteria sensing in food processing facilities. In: Encyclopedia of Agricultural, Food, and Biological Engineering, Third Edition. Eds: D.R. Heldman, C.I. Moraru, Taylor & Francis.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Oliveira, D. A., Hills, K., Giacobassi, C., Cavallaro, N., McLamore, E. S., Gomes, C. L., 2018. Nanosensors for food safety applications: biomimetic approaches. American Chemical Society (ACS) Midwest Regional Meeting, Ames, IA, October.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Kalia, A., Gomes, C. L. 2018. Synthesis and characterization of urea-chitosan nanoparticles with controlled release properties and improved fertilizer applications. QEEN II: 2nd Quantifying Exposure to Engineered Nanomaterials from Manufactured Products Workshop, Washington, DC, October.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Stromberg, L. R., Parate, K., Hondred, J. L., Rangekar, S. V., Hersam, M. C., Gomes, C., Claussen, J. 2018. Printed graphene interdigitated electrodes for bacterial pathogen biosensing. Conference of Food Engineering (CoFE), Minneapolis, MN, September.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Oliveira, D. A., McLamore, E. S., Gomes, C. 2018. Aptamer based biosensor for real-time detection of Listeria monocytogenes using pH-responsive polymer nanobrush and metallic nanoparticle platforms. Conference of Food Engineering (CoFE), Minneapolis, MN, September.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Summerlin, H., Pola, C., Borel, K., Chamakura, K. R., Young, R., Lacey, R., Vaddiraju, S., McLamore, E. S., Karthikeyan, R., Gomes, C. L. 2018. Fate, transport, and risk assessment study of norovirus and rotavirus during leafy greens (romaine lettuce) production using treated municipal wastewater. Conference of Food Engineering (CoFE), Minneapolis, MN, September.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Pola, C. C., Moraes, A. R. F., Teofilo, R., Medeiros, E. A. A., Soares, N. F. F., Gomes, C. L. 2018. Development and optimization of biodegradable nanocomposite based on corn starch and poly(vinyl alcohol) blend incorporated with nanocellulose for food packaging applications. Conference of Food Engineering (CoFE), Minneapolis, MN, September.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Zha, Y., Pola, C., Martinez, A., Summerlin, H., Johnson, J., Althawab, S., Oliveira, D., Pant, S., Gomes, C. L. 2018. Encapsulation of Chinese plant extracts using hydroxypropyl-beta-cyclodextrin and chitosan for antibacterial delivery applications. Conference of Food Engineering (CoFE), Minneapolis, MN, September.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Martinez-Rosales, A. F., Ramirez-Fuentes, E., Trujillo-Tapia, M. N., Sanchez-Burgos, J., Pola, C. C., Hernandez-Rodriguez, A., Gomes, C. L. 2018. Microencapsulation of Fischerella sp. with biodegradable polymers for improved biofertilizer application. Institute of Food Technologists (IFT) annual meeting, Chicago, IL, July.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ozdemir, N., Pola, C. C., Zha, Y., Teixeira, B. N., Hill, L. E., Bayrak, A., Gomes, C. 2018. Preparation of black pepper oleoresin inclusion complexes based on beta-cyclodextrin for antioxidant and antimicrobial delivery applications using kneading and freeze drying methods: a comparative study. Institute of Food Technologists (IFT) annual meeting, Chicago, IL, July.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Pola, C. C., Moraes, A. R. F., Teofilo, R. F., Medeiros, E. A. A., Soares, N., F. F., Gomes, C. L. 2018. Development and optimization of a biodegradable nanocomposite based on corn starch poly(vinyl alcohol) blend incorporated with nanocellulose for food packaging applications. Institute of Food Technologists (IFT) annual meeting, Chicago, IL, July.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Althawab, S. S., Oliveira, D. A., McLamore, E. S., Gomes, C. L. 2018. Real-time detection of Listeria monocytogenes using pH-responsive polymer nanobrushes and metallic nanoparticle platforms. World Congress on Biosensors 2018, Miami, FL, June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Cavallaro, N. D., Gomes, C., McLamore, E. S. 2018. Laser scribed graphene sensors for point of use detection of Listeria monocytogenes. World Congress on Biosensors 2018, Miami, FL, June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Oliveira, D. A., McLamore, E. S., Gomes, C. L. 2018. Nano-engineered pH-sensitive nanobrushes embedded with platinum nanoparticles for rapid detection of Listeria monocytogenes. World Congress on Biosensors 2018, Miami, FL, June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Giacobassi, C. A., Oliveira, D. A., Cavallaro, N., McLamore, E. S., Gomes, C. L. 2018. Real-time, label-free detection of Escherichia coli spp. Using electrochemical biosensors based on temperature-sensitive brushes and carbon-metal nanostructures. Nanoscale Science and Engineering for Agriculture and Food Systems - Gordon Research Conference  South Hadley, MA, June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Stromberg, L. R., Hondred, J. H., Oliveira, D., Medivelso, D., Smith, E., Gomes, C., Claussen, J. 2018. Printed graphene interdigitated electrode aptasensor for in-field Salmonella biosensing. Iowa State Research Day, Ames, IA, April.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Oliveira, D. A., McLamore, E. S., Gomes, C. Nanoengineered pH-sensitive polymer-platinum nanoparticle biosensor for rapid detection of Listeria monocytogenes. 2018 Institute of Biological Engineering (IBE), Norfolk, VA, April.
  • Type: Theses/Dissertations Status: Published Year Published: 2018 Citation: Johnson, J. 2018. Selective separation of proteins using stimuli-responsive polymers in an aqueous system. Texas A&M University.


Progress 10/31/16 to 09/30/17

Outputs
Target Audience:Graduate and undergraduate students, faculty, industry, stake holders, scientific community. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Mentoring of graduate and undergraduate students and visiting scholar were conducted as part of this project. Graduate and undergraduate students were trained on new methodologies for encapsulating natural antimicroibal compounds, microbiology analysis, and physico-chemical analysis of these delivery systems. Furthermore, students and visiting scholars were trained on new methodologies for biosensors design utilizing nano-hybrid materials (graphene and nanoplatinum), nano-metal deposition using sonoelecgrodeposition techniques, stimuli-responsive polymer and aptamer and lectin functionalization for real-time detection of foodborne pathogens. Events: PI presented 2017 (Materials Research Society) Fall Meeting and Exhibition, Boston, November. PI presented 2017 ASME IMECE (International Mechanical Engineering Congress and Exposition), Tampa, November PI attended the 2017 annual IFT international meeting, Las Vegas, June. PI organized and moderated a scientific symposium during 2017 annual IFT meeting on Challenges and Opportunities for Commercial Biosensors, Las Vegas, June. Graduate students and visiting scholar presented at IFT International Meeting, Las Vegas. June. PI presented at 2017 Institute of Biological Engineering, Salt Lake City. April. PI presented at 2017 Nanotech Technconnect World Innovation Conference and Expo. Washington, DC, May. Graduate students and visiting scholars have been trained in biological safety and working with biohazards at Texas A&M University. How have the results been disseminated to communities of interest?Results have been disseminated throughout peer-reviewed publications, PI's website, and presentations at international conferences. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue working toward the goals by conducting experiments, interpreting the data and disseminating the results throghout peer-reviewed publications, PI's website, and presentations at international conferences.

Impacts
What was accomplished under these goals? Reviewed emerging biorecognition and transduction schemes for rapid detection of pathogenic bacteria in food Assessed and discussed food processing and waste within the nexus framework. Developed water-tolerant core/shell upconversion nanoparticles for optical temperature sensing. Assessed the effects of nano-encapsulated natural antimicrobial application on quality of fresh-cut romaine lettuce. A comparative study of natural antimicrobial delivery systems for microbial safety and quality of fresh-cut lettuce was carried out over 15 days of storage at 5oC. Developed encapsulation methods of passion fruit seed oil by means of supercritical antisolvent process. Developed methods of Nanoencapsulation of passion fruit by-products extracts for enhanced antimicrobial activity. Developed rapid and label-free detection of interferon gamma via an electrochemical aptasensor comprised of a ternary surface monolayer on a gold interdigitated electrode array. Reviewed biosensors for indirect monitoring of foodborne bacteria. Developed High efficiency upconversion nanophosphors for high-contrast bioimaging. Reviewed synthesis methods and applications of cellulose nanohybrid materials. Developed Biomimetic Fractal Nanometals as a Transducer Layer in Electrochemical Biosensing Reviewed Microbial Pathogen Detection Strategies Developed Label-free, rapid Listeria monocytogenes biosensor based on a stimulus response nanobrush and nanometal hybrid electrode. Evaluated foodborne and antibiotic resistant pathogens prevalence during fresh produce production using non-traditional water sources regarding their fate, transport, and risk assessment analysis.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Vanegas, D. C., Gomes, C., Cavallaro, N. D., Giraldo-Escobar, D., McLamore, E. S. 2017. Emerging biorecognition and transduction schemes for rapid detection of pathogenic bacteria in food. Comprehensive Reviews in Food Science and Food Safety. 16 (6): 1188-1205.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Castell-Perez, E., Gomes, C., Tahtouh, J., Moreira, R., McLamore, R., Knowles, H. S. 2017. Food processing and waste within the nexus framework. Current Sustainable/Renewable Energy Reports. 4(3): 99-108.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Alkahtani, M. H., Gomes, C., Hemmer, P. R. 2017. Engineering water-tolerant core/shell upconversion nanoparticles for optical temperature sensing. Optics Letters. 42(13): 2451-2454.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Hill, L. E., Oliveira, D. A., Hills, K., Giacobassi, C., Johnson, J., Summerlin, H., Taylor, T. M., Gomes, C. 2017. A comparative study of natural antimicrobial delivery systems for microbial safety and quality of fresh-cut lettuce. Journal of Food Science. 82 (5): 1132-1141.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Oliveira, D. A., Mezzomo, N., Gomes, C., Ferreira, S. R. S. 2017. Encapsulation of passion fruit seed oil by means of supercritical antisolvent process. The Journal of Supercritical Fluids. 129: 96-105.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Oliveira, D. A., Angonese, M., Ferreira, S. R. S., Gomes, C. 2017. Nanoencapsulation of passion fruit by-products extracts for enhanced antimicrobial activity. Food and Bioproducts Processing. 104: 137-146.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ding, S.; Mosher, C.; Lee, X.; Das, S.; Cargill, A.; Tang, X.; Chen, B.; McLamore, E. S.; Gomes, C.; Hostetter, J.; Claussen, J. 2016. Rapid and label-free detection of interferon gamma via an electrochemical aptasensor comprised of a ternary surface monolayer on a gold interdigitated electrode array. ACS Sensors. 2(2): 210-217.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Vanegas, D. C., Gomes, C., McLamore, E. S. 2016. Biosensors for indirect monitoring of foodborne bacteria. Biosensors Journal. 5:137.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Alkahtani, M. H., Alghannam, F. S., Sanchez, C., Gomes, C., Liang, H. Hemmer, P. R. 2016. High efficiency upconversion nanophosphors for high-contrast bioimaging. Nanotechnology. 27 (48): 485501.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Garland, N. T., McLamore, E. S., Gomes, C., Marrow, E. A., Daniele, M. A., Walper, S., Medintz, I., Claussen, J. C. 2017. Synthesis and applications of cellulose nanohybrid materials. In: Hybrid Polymer Compositte Materials, Volume 4: 289-320.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: McLamore, E.S., M. Convertino, I. Oksoy, M. Taguchi, D.C. Vanegas, C. Gomes, J.C. Claussen. 2016. Biomimetic Fractal Nanometals as a Transducer Layer in Electrochemical Biosensing. In: Semiconductor Device-Based Sensors for Gas, Chemical, and Biomedical Applications. Ed by Fan Ren and Stephen J Pearton, CRC Press; ISBN: 1439813876
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Vanegas, D.C. J.C. Claussen, E.S. McLamore, C. Gomes. 2016. Microbial Pathogen Detection Strategies. In: Encyclopedia of Agricultural, Food, and Biological Engineering, Third Edition. Eds: D.R. Heldman, C.I. Moraru, Taylor & Francis.
  • Type: Theses/Dissertations Status: Published Year Published: 2017 Citation: Pant, S. 2017. Report on R&D manufacturing engineering internship at Post Consumer Brands.
  • Type: Theses/Dissertations Status: Published Year Published: 2017 Citation: Summerlin, H. 2017. Foodborne and antibiotic resistant pathogens prevalence during fresh produce production using non-traditional water sources: fate, transport, and risk assessment study.
  • Type: Theses/Dissertations Status: Published Year Published: 2017 Citation: Althawab, S. 2017. Aptamer-based biosensors for real-time detection of Listeria monocytogenes using pH-responsive polymer nanobrushes and metallic nanoparticle platforms.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Althawab, S., Oliveira, D.A., Smith, C., Cavallaro, N., McLamore, E. S., Gomes, C. 2017. Label-free, rapid Listeria monocytogenes biosensor based on a stimulus response nanobrush and nanometal hybrid electrode. Biotech, Biomaterials and Biomedical, TechConnect Briefs 2017. Sensor  Chemical, Physical & Bio, Chapter 7, 279-282. ISBN: 978-0-9988782-0-1
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Althawab, S., Oliveira, D. A., Rua, L., Cavallaro, N., McLamore, E. S., Gomes, C. L. 2017. Aptamer based biosensors for real-time detection of foodborne pathogens using pH-responsive polymer nanobrushes and platinum nanoparticles platforms. 2017 MRS (Materials Research Society) Fall Meeting and Exhibition, Boston, November.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Oliveira, D. A., Althawab, S., Cavallaro, N., McLamore, E. S., Gomes, C. L. 2017. Nanoengineering biomimetic nanobrushes for foodborne pathogen sensing using stimuli-response polymers and metallic nanostructures. 2017 ASME IMECE (International Mechanical Engineering Congress and Exposition), Tampa, November.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Oliveira, D. A., Smith, C., McLamore, E. S., Gomes, C. 2017. Nano-engineered pH-sensitive polymer nanobrushes embedded with platinum nanoparticles for rapid detection of Listeria monocytogenes in food applications. IFT International Meeting, Las Vegas. June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Summerlin, H., Abuhamad, J., Pola, C. C., Chacon, K., Gomes, C. L. 2017. Foodborne and Antibiotic Resistant Pathogen Prevalence During Fresh Produce Production Using Non-Traditional Water Sources: Fate and Transport Study. IFT International Meeting, Las Vegas. June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Pola, C. C., Oliveira, D. A., Martinez-Rosales, A. F., Oh, J. K., Mustafa, A., Soares, N. F. F., Gomes, C. L. 2017. Optimization of pH-responsive nanoparticles based on Poly-D, L-lactide-co-glycolide (PLGA), chitosan and trans-cinnamaldehyde for antimicrobial delivery applications. IFT International Meeting, Las Vegas. June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Althawab, S. Oliveira, D. A., Pant, S., McLamore, E. S., Gomes, C. 2017. Aptamer Based Biosensor for Real-time Detection of Listeria monocytogenes in Food Products Using pH-responsive Polymer Nanobrushes and Metallic Nanoparticles Platforms. IFT International Meeting, Las Vegas. June.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: McLamore, E. S., Khondaker, I., Gomes, C., Oliveira, D. A. 2017. Stimulus-response biosensor for determining bacteria viability using lectin-glycoenzyme nanobrushes. Nanotech Technconnect World Innovation Conference and Expo. Washington, DC, May.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Oliveira, D. A., Althawab, S., Smith, C., McLamore, E. S., Gomes, C. 2017. Real-time monitoring of Listeria monocytogenes based on composites of pH-responsive polymer nanobrushes, nanoplatinum, and aptamers. Institute of Biological Engineering, Salt Lake City. April.