Source: UNIVERSITY OF ARKANSAS submitted to
NANOTECHNOLOGY AND BIOSENSORS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1011247
Grant No.
(N/A)
Project No.
ARK02545
Proposal No.
(N/A)
Multistate No.
NC-_old1194
Program Code
(N/A)
Project Start Date
Oct 1, 2016
Project End Date
Sep 30, 2021
Grant Year
(N/A)
Project Director
Li, YA.
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
Biological & Agr Engineering
Non Technical Summary
Novel biosensors will be developed based on nanomaterials and nanostructures for more rapid and more sensitive detection of pathogenic bacteria and viruses in agricultural and food products. This will provide the food and agricultural industries advanced detection technologies to ensure food safety and control animal diseases.
Animal Health Component
30%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
4045010202025%
7124010110025%
3114030110125%
7113299100025%
Knowledge Area
311 - Animal Diseases; 711 - Ensure Food Products Free of Harmful Chemicals, Including Residues from Agricultural and Other Sources; 712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins; 404 - Instrumentation and Control Systems;

Subject Of Investigation
3299 - Poultry, general/other; 4010 - Bacteria; 4030 - Viruses; 5010 - Food;

Field Of Science
2020 - Engineering; 1101 - Virology; 1100 - Bacteriology; 1000 - Biochemistry and biophysics;
Goals / Objectives
Develop devices and systems incorporating microfabrication and nanotechnology Develop/improve education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment Improve academic-industry partnership to help move the developed technologies to commercialization phase
Project Methods
The overall goal of this project is to develop nanotechnology-based biosensors that will meet the required sensitivity, specificity, and speed for screening of pathogenic bacteria and viruses in different food and agricultural samples. The specific objectives of this research are: (1) Develop new technologies for characterizing fundamental nanoscale processes. Develop a bioseparation method based on magnetic nanoparticles coated with specific antibodies/aptamers to separate target bacteria or virus in a food or agricultural sample and concentrate them for more effective detection; (2) Construct and characterize self-assembled nanostructures. Design and fabricate bio-nanowire/nanotube/nanopore/nanoparticles as biosensing materials to improve detection sensitivity and reduce assay time; and (3) Develop devices and systems incorporating microfabrication and nanotechnology. Evaluate the biosensor for in-field, rapid detection of different pathogenic bacteria in different food samples, and different viruses in poultry and other agricultural animals.The biosensor consists of a sampler, multiple-section microfluidic cartridges, a pumping unit, an electrochemical or optical detector, a microprocessor, a display, a key panel, and a USB connector. When a food or poultry sample, containing various biological and chemical components with bacteria or viruses, is dropped, it is mixed with magnetic nanobeads coated with antibodies/aptamers for several min to get sufficient bioreaction to capture target bacteria or viruses. Then, the target bacteria or viruses are separated by applying a magnetic field to hold magnetic bio-nanoparticles while washing. During their flowing through a microfluidics channel, target bacteria or viruses are captured by the antibodies/aptamers immobilized on the nanowire/nanoparticle/nanopore surface. Free nanobeads and others can pass through the channel. The change in electrochemical or optical signals, caused by captured target bacteria or viruses, is measured and correlated to the concentration of target bacteria or viruses in a sample. A research prototype of nano-biosensor will be designed, fabricated, and tested. The nano-biosensor will be further optimized, improved and evaluated for its applications in agriculture and food systems.

Progress 10/01/16 to 09/30/21

Outputs
Target Audience:Agricultural and food producers, processors, distributors, wholesale and retail sellers, consumers, regulatory agencies, analytical laboratories. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project provided an opportunity for training threePh.D. students and two M.S. students as well as three postdoctoral researchers in the areas of biosensors and nanotechnology. The project also enhanced our multidisciplinary research in biosensors for food safety and our collaborations with professionals in molecular biology, analytical chemistry, material science, food science, mechanicalengineering and electrical engineering. We taught "biosensors and bioinstrumentation" in both undergraduate and graduate courses. How have the results been disseminated to communities of interest?For the past five years, we made a total of 17presentations to the industry and scientific communities at professional conferences such as ASABE, IAFP, and IFT annual meetings. We havepublished more than 30articles in highly ranked journals including Food Control, Poultry Science, Transactions of the ASABE, Biosensors & Bioelectronics, Sensors and Actuators, Electroanalysis, etc. Amid the covid pademic, we presented the results of biosensor research to both industry and academia by several webinars, which helped communications with more companies and research institutes. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Nanoparticles-based immunomagnetic separation and quantum dots-based fluorescent labels were used in development of new biosensors for the rapid detection of pathogenic bacteria and antibiotics residues in agricultural and food products. Antibodies and aptamers were selected as biosensing materials in the design of novel electrochemical, optical, QCM biosensors. The nanomaterials-based biosensors were also integrated with microfluidic chips, 3D-printed measurement chambers, and cell phone-based data acquisition, processing, and transmission. These nanomaterials-based biosensors have shown their potential to be applied to the in-field or online detection of biological and chemical agents in agricultural and food products for ensuring food quality and safety. A research prototype of the biosensing instrument for detection of Salmonella Typhimurium in food products is designed, fabricated, and evaluated. The implementation of these biosensors will provide close to real-time monitoring of food safetyfor entire food supply chain and provide the critical data for the development of AI-driven and climate-smartagricultural and food systems.

Publications

  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Liang, A.M., Y.F. Shen, Y.W. He, J.P. Wang and Y. Li. 2021. An automated magnetic separation device coupled with a fluorescent biosensor for the detection of antibiotics residues. Transactions of the ASABE 64(1): 23-30. doi: 10.13031/trans.14076
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: McLamore, E.S., E. Alcocilja, C. Gomes, &Y. Li, et al. 2021. FEAST of biosensors: Food, environmental and agricultural sensing technologies (FEAST) in North America. Biosensors & Bioelectronics 178, 113011. doi.org/10.1016/j.bios.2021.113011
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Wang, W.Q., R.H. Wang, M. Liao, M.T. Kidd, and Y. Li. 2021. Rapid detection of enrofloxacin using a localized surface plasmon resonance sensor based on polydopamine molecular imprinted recognition polymer. Journal of Food Measurement and Characterization 15(4): 3376-3386. DOI:10.1007/s11694-021-00913-x
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Xi, X.G., I.I. Niyonshuti, N.X. Yu, L. Yao, Y. Fu, P. Yao, J.Y. Chen, and Y. Li. 2021. A label-free QCM biosensor based on target-triggered release of cargo molecules in gold nanocages capped with aptamers for thrombin detection. ACS Applied Nano Materials 4(10): 1004710054. DOI:10.1021/acsanm.1c01350
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Xue, L., N.N. Jin, Guo, R.Y., S.Y. Wang, W.Z. Liu, Y.J. Liu, Y. Li, and J.H. Lin. 2021. Microfluidic colorimetric biosensors based on MnO2 nanozymes and convergence-divergence spiral micromixers for rapid and sensitive detection of Salmonella. ACS Sensors 6(8): 2883-2892. doi.org/10.1021/acssensors.1c00292
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Shen, Y.F., L.Z. Xu and Y. Li*. 2021. Biosensors for rapid detection of Salmonella in food: A review. Comprehensive Reviews in Food Science and Food Safety 20:149-197. DOI: 10.1111/1541-4337.12662
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Peng, Y.P., Y.W. He, Y.F. Shen, A.M. Liang, X.B. Zhang, Y.J. Liu, J.H. Lin, J.P. Wang, Y. Li, Y.C. Fu. 2021. Fluorescence nanobiosensor for simultaneous detection of multiple veterinary drugs in chicken samples. Journal of Analysis and Testing (published November 17, 2021) doi.org/10.1007/s41664-021-00199-4
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Jia, F., X.J. Bai, X.W. Zhang, Y.C. Fu, Y. Li, X.M. Li, and J. Kokini. 2021. A low-field magnetic resonance imaging aptasensor for the rapid and visual sensing of Pseudomonas aeruginosa in food, juice and water. Analytical Chemistry 93(24): 8631-8637. doi.org/10.1021/acs.analchem.1c01669
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Li, Z.S., X.H. Xu, H.R. Quan, J. Zhang, Q. Zhang, Y.C. Fu, Y.B. Ying, and Y. Li. 2021. Adsorptive and responsive hybrid sponge of melamine foam and metal organic frameworks for rapid collection/removal and detection of mycotoxins. Chemical Engineering Journal 410: 128268. doi.org/10.1016/j.cej.2020.128268
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Wang, W.Q., M. Kidd, and Y. Li. 2021. Localized surface plasmon resonance biosensor based on polydopamine molecular imprinted polymer for detection of multi-antibiotics in chicken meat. A poster presented at the IAFP 2021 Annual Meeting, July 18-21, 2021, Phoenix, AZ.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Xi, X.G., N.X. Yu, I. I. Niyonshuti, L. Yao, Y. Fu, J.Y. Chen, and Y. Li. 2021. An ultrasensitive and label-free quartz crystal microbalance biosensor based on gold nanocages capped with aptamers for rapid detection of thrombin. Presented at ASABE 2021 Annual International Meeting, July 12-16, 2021, virtual meeting online. Paper No. 2101092.
  • Type: Theses/Dissertations Status: Published Year Published: 2021 Citation: Xi, X.G. 2021. Nanomaterial-based Biosensors for Detection of Salmonella Typhimurium and Avian Influenza Virus H5N1 in Poultry. Ph.D. dissertation, University of Arkansas, Fayetteville, AR.


Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Agricultural and food producers, processors, distributors, wholesale and retail sellers, consumers, regulatory agencies, analytical laboratories. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project provided an opportunity for trainingtwo Ph.D. students and one M.S. student in the areas of biosensors and nanotechnology. The project also enhanced our multidisciplinary research in biosensors for food safetyandour collaborations with professionalsin molecular biology, analytical chemistry, food science and biological engineering. How have the results been disseminated to communities of interest?We mademore than 8 presentations to the industry and scientific communities at professional conferences such as ASABE, IAFP, and IFT annual meetings. We also published 7 articles in highly ranked journals including Food Control, Poultry Science, Transactions of the ASABE, Biosensors & Bioelectronics, Electroanalysis, etc. What do you plan to do during the next reporting period to accomplish the goals?We will continue to work on (1) development of innovative nano-biosensors for rapid detection of foodborne pathogens and chemical residues in food supply chains; (2) education and training of undergraduate, graduate students andpostdoctoral associateson biosensors and bioinstruments with nanotechnology and biotechnology; and (3) commercialization of nano-biosensing technology in collaboration with the industry including Walmart and Tyson Foods.

Impacts
What was accomplished under these goals? Magnetic nanoparticles and quantum dots wereused in development of new biosensors for the rapid detection of pathogenic bacteria and antibiotics residues in agricultural and food products.Antibodies and aptamers were selected as biosensing materials in the design ofnovel electrochemical, optical, QCMbiosensors. The nanomaterials-based biosensors were also integrated with microfluidic chips, 3D-printed measurement chambers,and cell phone-based data acquisition, processing, and transmission. These nanomaterials-based biosensors have shown their potential to be applied to the in-field or online detection of biological and chemical agents in agricultural and food products for ensuring food quality and safety. The implementation of these biosensorswill provide close to real-time data for the development of smart agricultural and food systems based on big data analytics and machine learning.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: He, Y.W., F. Jia, J.F. Guan, Y.C. Fu, and Y. Li. 2020. Electrochemical conversion of magnetic nanoparticles using disposable working electrode in a 3D-printed electrochemical cell. Electroanalysis 32(7):1426-1432.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Qiao, Z.H., Yingchun Fu, Chunyang Lei, Y. Li. 2020. Advances in antimicrobial peptides-based biosensing methods for detection of foodborne pathogens: A review. Food Control 112(6):107116. doi.org/10.1016/j.foodcont.2020.107116
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Wang, L., L. Xue, R.Y. Guo, L.Y. Zheng, S.Y. Wang, L. Yao, X.T. Huo, N. Liu, M. Liao, Y. Li, and J.H. Lin. 2020. Combining impedance biosensor with immunomagnetic separation for rapid screening of Salmonella in poultry supply chains. Poultry Science 99:1606-1614. doi.org/10.1016/j.psj.2019.12.007
  • Type: Journal Articles Status: Accepted Year Published: 2020 Citation: Xi, X.G., R.H. Wang, P. Yao, S. Tung, Y. Li. 2020. A portable biosensing device with magnetic separation and quantum dot bead labeling for simple, rapid and quantitative detection of Salmonella Typhimurium. Transactions of the ASABE (accepted August 16, 2020). doi: 10.13031/trans.13880
  • Type: Journal Articles Status: Accepted Year Published: 2020 Citation: Liang, A.M., Y.F. Shen, Y.W. He, J.P. Wang and Y. Li. 2020. An automated magnetic separation device coupled with a fluorescent biosensor for the detection of antibiotics residues. Transactions of the ASABE. (accepted 8-29-2020). doi: 10.13031/trans.14076
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Wang, W.Q., M. Kidd, and Y. Li. 2020. Application of polydopamine molecular imprinted polymer on a localized surface plasmon resonance sensor for detection of multi-antibiotics in chicken meat. A poster presented at IAFP 2020 Annual Meeting, October 25-28, 2020, virtual meeting online. Paper No. P1-115.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Shen, Y.F., F. Jia, A.M. Liao, H. Dai, Y.P. Peng, Y.C. Fu, and Y. Li. 2020. A novel optical biosensor based on target-induced immunomagnetic beads aggregation for one-step and portable detection of enrofloxacin. A poster presented at IAFP 2020 Annual Meeting, October 25-28, 2020, virtual meeting online. Paper No. P1-46.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Shen, Y.F., Z. Lu, Y.W. He, A.M. Liang, Y.C. Fu, J.P. Wang, and Y. Li. 2020. A portable fluorescent biosensor with smartphone readout for rapid detection of enrofloxacin in poultry supply chain. A poster presented at IFT 2020 Annual Meeting, July 13-15, 2020, virtual meeting online.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Jia, F., X.J. Bai, X.W. Zhang, R.T. Dai, J. Kokini, Y. Li, and X.M. Li. 2020. A robust magnetic relaxation switch (MRSw) aptasensor for the reliable and visual sensing of Pseudomonas aeruginosa in food and drinking water. A poster presented at ASABE 2020 Annual International Meeting, July 12-15, 2020, virtual meeting.
  • Type: Journal Articles Status: Submitted Year Published: 2020 Citation: Wang, W.Q., R.H. Wang, M. Liao, M.T. Kidd, and Y. Li. 2020. Rapid detection of enrofloxacin using a localized surface plasmon resonance sensor based on polydopamine surface imprinted recognition polymer. Journal of Food Measurement and Characterization (submitted 8-24-2020)
  • Type: Journal Articles Status: Submitted Year Published: 2020 Citation: Yu, X.F., R.H. Wang, A. Al-Ogaili, T.S. Jiang, Y.M. Kwon, M. Ivey, J.C. Zhou, and Y. Li. 2020. Ultra-sensitive and rapid detection of E. coli O157:H7 using a QCM sensor based on a multivalent aptamer system. Sensing and Bio-sensing Research (submitted 8-7-2020)
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Xu, L.Z., D.Y. Li, S. Ramadan, Y. Li, and N. Klein. 2020. Facile biosensors for rapid detection of COVID-19. Biosensors & Bioelectronics 170: 112673. doi.org/10.1016/j.bios.2020.112673


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Food producers, food processors, govenment regulatory agencies, consumers, nanomaterials companies, instrumentation companies Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Graduate students and postdoctoral research associates have got the good oppotunities to design, fabricate and evaluate innovative biosensors for rapid detection of pathogens, antibiotics residues, pesticide residues and more biological and chemical contaminants in agriculture, food, and environment through their participating in the projects on biosensors. They have also learned more about nanotechnology, and applied nanomaterials to the development of novelbiosensors through their conducting the projects on nanomaterials-based biosensors. These helped the garduate students and postdoctoral research associate in their professional training and career development. How have the results been disseminated to communities of interest?The research results have been presented in international meetings including ASABE, IAFP, ACS, SRA, Pittcon and more, and published in highly ranked journals such as Biosensors and Bioelectronics, Sensors and Acuators, Transactions of the ASABE, Journal of Food Protection, and Biochemical Analysis. At the same time, we have visited the food indutry including Tyson Food and Walmart to present our research results and discussed potential collaboration for applications of the biosensors. What do you plan to do during the next reporting period to accomplish the goals?(1) We will continue to work on these impedance, optical, SPR and QCM biosensors for rapid detection of foodborne pathogens and antibiotic residues and extend their detection functions to more target pathogens and antibiotics. (2) We will continue to teach biosensors in both undergraduate and graduate courses and to present the research results in workshops and seminars. (3) we will continue to collaborate with food, instrumentation and nanomaterial companies to improve research prototypes of biosensors for applications in food and agricultural systems.

Impacts
What was accomplished under these goals? (1) Portable impedance and fluoresence biosensors have been studied for rapid detection of foodborne pathogens including Salmonella Typhimurium, Listeria monocytogenes and E. coli O157:H7 in food products and environment using microfluidic chips and nanomaterials such as magnetic nanoparticles and quantum dots. Portable SPR and optical biosensors have been studied for rapid detection of antibiotics residues in feed, water and poultry and meat products using nanomaterials such as gold nanoparticles and quantum dots. (2) In a graduate course on advanced instrumentation in biological engineering, biosensors and bioinstrumentation have been taught with lectures and laboratory for hand-on practice. Several presentations have been given in workshops or training classes to introduce biosensors and their applications in agriculture and food systems. (3) In collaboration with instrumentation companies, a research prototypeof the impedance biosensor for detection of foodborne pathogens and a research prototype of the optical biosensors for detection of antibiotics residues havebeen designed and constructed for use in research on food safety monitoring and risk assessment system of poultry supply chain.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: He, K.Y., Z.S. L. Wang, Y.C. Fu, H.R. Quan, Y. Li, X.Q. Wang, S. Gunasekaran, and X.H. Xu. 2019. A water-stable luminescent metal-organic framework for rapid and visible ssensing of organophosphorus pesticides. ACS Applied Materials & Interfaces 11(29): 26250-26260.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, Z.S., X.H. Xu, Y.C. Fu, Y.N. Guo, Q.Y. Zhang, H. Yang, and Y. Li. 2019. A water-stable luminescent metal-organic framework for effective detection of Aflatoxin B1 in walnut and almond beverages. RCS Advances 9:620-625.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Xiao, X.N., W. Wang, X.B. Zhang, J.M. Zhang, M. Liao, H. Yang, Q.Y. Zhang, C. Rainwater, and Y. Li. 2019. Modeling the reduction of Salmonella spp. on chicken breasts and wingettes during scalding for QMRA of the poultry supply chain in China. Microorganisms 7:165.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Xiao, X.N., W. Wang, J.M. Zhang, M. Liao, H. Yang, W.H. Fang, and Y. Li. 2019. Modeling the reduction and cross-contamination of Salmonella in poultry chilling process in China. Microorganisms 7:448.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Yao, P., R.H. Wang, X.G. Xi, Y. Li, and S. Tung. 2019. 3D-printed pneumatic microfluidic mixer for colorimetric detection of Listeria Monocytogenes. Transactions of the ASABE, 62(3):841-850.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Yu, N.X., X.Y. Wang, L. Qiu, T.M. Cai, C.J. Jiang, Y. Sun, Y. Li, H.L. Peng, and H. Xiong. 2020. Bacteria-triggered hyaluronan/AgNPs/gentamicin nanocarrier for synergistic bacteria disinfection and wound healing application. Chemical Engineering Journal 380:122582.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Yu, N.X., H.L. Peng, L. Qiu, R.H. Wang, C.J. Jiang, T.M. Cai, Y. Sun, Y. Li, and H. Xiong. 2019. New pectin-induced green fabrication of Ag-AgCl/ZnO nanocomposites for visible-light triggered antibacterial activity. International Journal of Biological Macromolecules 141:207-217
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Zheng, L.Y., G.Z. Cai, S.Y. Wang, M. Liao, Y. Li, and J.H. Lin*. 2019. A microfluidic colorimetric biosensor for rapid detection of Escherichia coli O157:H7 using gold nanoparticle aggregation and smart phone imaging. Biosensors & Bioelectronics 124-125: 143-149.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Zou, Q.Y., R. Wang and Y. Li. 2019. A minireview on eletrochemical biosensors for the detection of heavy metal ions in water. ASABE Paper No. 1901797. ASABE 2019 Annual International Meeting, July 7-10, 2019, Boston, MA.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Wang, R.H., X.F. Yu, T.S. Jiang, Y.M. Kwon, J.C. Zhao, M. Ivey, and Y. Li. 2019. A label-free QCM biosensor for sensitive and rapid detection of E. coli O157:H7 based on a multivalent aptamer system. Oral presentation at the IAFP 2019 Annual Meeting, July 21-24, 2019, Louisville, KY. Paper No. T5-05.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Xi, X.G., R.H. Wang, P. Yao, J.H. Lin, M. Liao, and Y. Li. 2019. A portable sensing device with magnetic separation and quantum dot labeling for simple, rapid and quantitative detection of Salmonella Typhimurium. ASABE Paper No. 1900564. An oral presentation at ASABE 2019 Annual International Meeting, July 7-10, 2019, Boston, MA.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Zhu, W.Y., Y.Q. Li, Y.C. Fu, and Y. Li. 2019. A nanobiosensor based on integrated nanochannel-electrode system for rapid detection of Salmonella in chicken products. ASABE Paper No. 1900995. An oral presentation at ASABE 2019 Annual International Meeting, July 7-10, 2019, Boston, MA.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Yao, L., L. Wang, N. Liu, S. Wang, Y. Li, J.H. Lin. 2019. Direct detection of Salmonella using inertial microfluidics-based separation and enzymatic catalysis-based colorimetry. Poster presentation at 6th International Conference on Biosensing Technology ( ICBT), June 16-19, 2019, Kuala Lumpur, Malaysia. Paper No. P2.68.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Lu, Z., Y.F. Shen, C.J. Li, Y. Li, Y.C. Fu, Z.Z. Ye, and J.P. Wang. 2019. Micro-optical components and quantum dots based handheld instrument system for in-field detection in poultry supply chain. Presented it at Pittcon 2018, March 17-21, 2019, Philadelphia, PA.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Li, Y. 2019. Biosensors for smart agriculture. An invited presentation at the 2nd China Forum on Biosensors, Biochips and Nanobiotech (BBN China 2019), May 24-25, 2019, Jinan, Shandong Province, China.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Li, Y. 2019. Poultry excellence in China: Improving food safety of poultry supply chain. An invited presentation at Global Food Safety Governance Forum, April 18, 2019, Beijing, China.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Wang, W.Q., R.H. Wang, M. Liao, and Y. Li. 2019. Rapid detection of enrofloxacin in poultry using a localized surface plasmon resonance sensor based on polydopamine surface imprinted recognition polymer. Oral presentation at the IAFP 2019 Annual Meeting, July 21-24, 2019, Louisville, KY. Paper No. T5-09.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Xiao, X.N., W. Wang, J.M. Zhang, M. Liao, H. Yang, Q. Wang, and Y. Li. 2019. A predictive model for cross-contamination of Salmonella in the poultry chilling process. Poster presentation at the IAFP 2019 Annual Meeting, July 21-24, 2019, Louisville, KY. Paper No. P1-166.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Agricultural and food procurers,producers,distributors, wholesale and retail sellers, consumers, regulatory agencies, analytical laboratories. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project provided an opportunity for training four Ph.D. students and one M.S. student in the areas of biosensors and nanotechnology. The project also enhanced our international collaboration with Zhejiang University and China Agricultural University on a project funded by Walmart Foundation. How have the results been disseminated to communities of interest?We made 15 presentations to the industry and scientific communities at professional conferences such as ASABE (American Society of Agricultural and Biological Engineers), ACS (American Chemical Society), IAFP (International Association for Food Protection), and IBE(Institute of Biological Engineering)annual meetings as well as Biosensors 2018. We also published 11 articles in different journals including Biosensors & Bioelectronics, Sensors & Actuators, Journal of Food Protection, Electroanalysis, Journal of Materials Chemistry, Journal of Electroanalytical Chemistry, Journal of Biotechnology, etc. What do you plan to do during the next reporting period to accomplish the goals?We will continue to work on (1) development of innovative nano-biosensors for rapid detection of pathogenic bacteria and viruses, toxins and chemical residues in food supply chains; (2) education and training of undergraduate and graduate students and industrial users on biosensors and bioinstruments based on nanotechnology and biotechnology; and (3) collaboration with the industry including a major retailerand a major consumer food productscompanyfor commercialization of nano-biosensing technologies.

Impacts
What was accomplished under these goals? Various nanoscale materials, including magnetic nanoparticles, silk nanofibers, gold nanorods, nanochannels, were synthesized or fabricated, characterized, and used in development of new biosensors for the rapid detection of pathogenic bacteria, viruses, toxic agents, and antibiotics residues in agricultural and food products. Enzymes, antibodies, aptamers and DNA probes were selected as biosensing materials and electrochemical, optical, QCM transducing methods were employed in the design of novel biosensors. The nanomaterials-based biosensors were also integrated with microfluidic chips-based sample treatment and measurement and cell phone-based data acquisition, processing, and transmission. These nanomaterials-based biosensors have shown their potential to be applied to the in-field or online detection of biological and chemical agents in agricultural and food products for ensuring food quality and safety. The implementation of these biosensors may provide close to real-time data in biodetection for the development of smart agricultural and food systems based on big data, machine learning and artificial intelligence.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Cao, L.L., Q. Zhang, H. Dai, Y.C. Fu, and Y. Li. 2018. Separation/concentration-signal-amplification in-one method based on electrochemical conversion of magnetic nanoparticles for electrochemical biosensing. Electroanalysis 30(3):517-524. DOI: 10.1002/elan.201700653
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Dai, H., Y.Q. Li, Y.C. Fu, and Y. Li. 2018. Enzyme catalysis induced polymer growth in nanochannels: A new approach to regulate ion transport and to study enzyme kinetics in nanospace. Electroanalysis 30(2):328-335 (available online Dec. 18, 2017). DOI:10.1002/elan.201700703
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Dai, H., Y.Q. Li, Q. Zhang, Y.C. Fu, and Y. Li. 2018. A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine. RSC Advances 8:33960-33967. DOI: 10.1039/c8ra06347h
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hu, Q.Q., R.H. Wang, H. Wang, M.F. Slavik and Y. Li. 2018. Selection of acrylamide-specific aptamers by a quartz crystal microbalance combined SELEX method and their application in rapid and specific detection of acrylamide. Sensors and Actuators: B: Chemical 273:220-227. doi.org/10.1016/j.snb.2018.06.033
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Li, Z.S., G.S. Zhou, H. Dai, M.Y. Yang, Y.C. Fu, Y.B. Ying, and Y. Li. 2018. Biomineralization-mimetic preparation of hybrid membranes with ultra-high load of pristine metal-organic frameworks grew on silk nanofibers for hazards collection in water. Journal of Materials Chemistry A 6(8):3402-3413 (published online on December 5, 2017). DOI:10.1039/C7TA06924C
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Wang, H., L.J. Wang, Q.Q. Hu, R.H. Wang, Y. Li and M. Kidd. 2018. Rapid and sensitive detection of Campylobacter jejuni in poultry products using a nanoparticles-based piezoelectric immunosensor integrated with magnetic immunoseparation. Journal of Food Protection 81(8):1321-1330. doi:10.4315/0362-028X.JFP-17-381
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Wang, L.J., R.H. Wang, H. Wei, and Y. Li. 2018. Selection of aptamers against pathogenic bacteria and their diagnostics application. World Journal of Microbiology and Biotechnology 34:149. doi.org/ 10.1007/s11274-018-2528-2
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Xu, C.N., L.Y. Lan, Y. Yao, J.F. Ping, Y. Li, and Y.B. Ying. 2017. An unmodified gold nanorods-based DNA colorimetric biosensor with enzyme-free hybridization chain reaction amplification. Sensors & Actuators: B. Chemical. 273:642-648. doi.org/:10.1016/j.snb.2018.06.035
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yu, X.F., F. Chen, R.H. Wang, and Y. Li. 2018. Whole-bacterium SELEX of DNA aptamers for rapid detection of E. coli O157:H7 using a QCM sensor. Journal of Biotechnology 266:39-49. (available online, Dec. 22, 2017).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Zhang, Q., L. Zhang, H. Dai, Z.S. Li, Y.C. Fu, and Y. Li. 2018. Biomineralization-mimetic preparation of robust metal-organic frameworks biocomposites film with high enzyme load for electrochemical biosensing. Journal of Electroanalytical Chemistry 823:40-46. doi.org/10.1016/j.jelechem.2018.04.015
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Shen, Y.F., Y.W. He, Y.C. Fu, J.P. Wang, J.H. Lin, M. Liao, and Y. Li. 2018. A nanomaterials-based biosensor for rapid detection of enrofloxacin in chicken products. ASABE Paper No. 1800703. ASABE 2018 Annual International Meeting, July 29-August 1, 2018, Detroit, MI. doi.org/10.13031/aim.201800703.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Sotero, A., R.H. Wang, T. Wen, B. Zhang, and Y. Li. 2018. A portable impedance aptasensing system for rapid detection of Salmonella Typhimurium in poultry products. ASABE Paper No. 1801147. ASABE 2018 Annual International Meeting, July 29-August 1, 2018, Detroit, MI. doi.org/10.13031/aim.201801147
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Yao, P., N. Harris, R.H. Wang, X.G. Xi, J.Y. Wang, Y. Li, and S. Tung. 2018. 3D-printed pneumatic microfluidic mixer for colorimetric detection of Listeria monocytogenes. ASABE Paper No. 1800524. ASABE 2018 Annual International Meeting, July 29-August 1, 2018, Detroit, MI. doi: 10.13031/aim.201800524
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Xi, G.X., R.H. Wang, Z. Chen, and Y. Li. 2018. Surface imprinted polydopamine based magnetic separation and quantum dots based fluorescent biosensor for detection of foodborne pathogenic bacteria. ASABE Paper No. 1801203. ASABE 2018 Annual International Meeting, July 29-August 1, 2018, Detroit, MI. doi: 10.13031/aim.201801203
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Fu, Y.C., L.L. Cao, Q. Zhang, H. Dai, and Y. Li. 2018. Exploring more for magnetic nanoparticles: Separation/concentration-signal-generation in one method for biosensing. Presented at the 28th Anniversary World Congress on Biosensors (Biosensors 2018), June 12-15, 2018, Miami, FL. Paper No. P1.123.
  • Type: Theses/Dissertations Status: Published Year Published: 2018 Citation: Yu, Xiaofan. 2018. Rapid and Sensitive Detection of Escherichia coli O157:H7 Using a QCM Sensor based on Aptamers Selected by Whole-Bacterium SELEX and a Multivalent Aptamer System. PhD Dissertation, University of Arkansas, Fayetteville, AR.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Wang, R.H., W.Q. Wang, J.M. Zhang, M. Liao, and Y. Li. 2018. Rapid and sensitive detection of Salmonella Typhimurium using an LSPR sensor based on polydopamine surface imprinted recognition polymer. Presented at the 28th Anniversary World Congress on Biosensors (Biosensors 2018), June 12-15, 2018, Miami, FL. Paper No. P2.198
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Wang, W.Q., R.H. Wang, and Y. Li. 2018. An LSPR sensor coupled with magnetic nanobeads-based immunoseparation for rapid and sensitive detection of E. coli O157:H7. Presented at the IAFP 2018 Annual Meeting, July 8-11, 2018, Salt Lake City, UT. Paper No. P2-152.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Xiao, X.N., W. Wang, J.M. Zhang, M. Liao, Y. Li, G.L. Yang, H. Yang, Q. Wang, and C. Rainwater. 2018. Quantitative risk assessment of Salmonella for yellow broiler supply chain in China. Presented at the IAFP 2018 Annual Meeting, July 8-11, 2018, Salt Lake City, UT. Paper No. P3-111.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Yu, X.F., R.H. Wang, T.S. Jiang, Y.M. Kwon, and Y Li. 2018. Sensitive and rapid detection of E. coli O157:H7 using a QCM sensor based on a multivalent aptamer system. A poster presented at IBE 2018 Annual Meeting, April 6-7, 2018, Norfolk, SC.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Zhang, Q., L. Li, Y.C. Fu, Q. Xie, and Y. Li. 2018. Electrochemical conversion of magnetic nanoparticles to develop multi-template method to prepare porous nanocomposites for biosensing. Presented at the 28th Anniversary World Congress on Biosensors (Biosensors 2018), June 12-15, 2018, Miami, FL. Paper No. P2.048.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Poultry producers and processors, food producers and processors, and analytical laboratories. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project provided the training in biosensor research to five graduate students, three in biological engineering, one in cell and molecular biology, and one in poultry science, and the professional development to one postdoctoral research associate in analytical chemistry.The project also provided the interdisciplinary training in biodetection technologiestoone joint-training Ph.D. student in biosystems engineering andthree visiting scholars in food science and food engineering.During conducting this project, an interdisciplinary and/or multidisciplinary environment was created for researchers from different disciplines, including analytical chemistry, microbiology, food science,and biological engineering, to work together in the same researchlaboratoryto generate new ideas for developing innovative nanomaterials-based biosensors with approaches from both science and engineering. How have the results been disseminated to communities of interest?The research results have been presented at American Society of Agricultural and Biological Engineers (ASABE) 2017 annual international meeting, International Association of FoodProtection (IAFP) 2017 annual meeting, American Chemical Society (ACS) 2017 Fall meeting and other international meetings.Over tenpeer-reviewed articles were published in the journals ofTransactions of the ASABE, Sensors and Actuators, Sensors, Food Chemistry, Talanta,Journal of Biotechnology and others. Invited presentations were given atInternational Symposium SmartAg 2017, ACS 2017 andBBN China 2017. What do you plan to do during the next reporting period to accomplish the goals?First, continue conducting more experiments to obtain more dataincapture, separation and detectionusing nanotechnology and biosensors. Secondly, continue working on the design and fabrication of an automated biosensing device for in-field or online use to simultaneously detect multiple foodborne pathogens. The prototype devises and instruments should be portable,easy for operation and low cost. Thirdly, continue to design and fabricatenanomaterials-based biosensors for more sensitive and rapid detection of avian influenza viruses in poultry and bacterial pathogens andpesticide andantibioticsresiduesin food products.

Impacts
What was accomplished under these goals? For Objective 1, microfluidic chips and microflow cells have been designed and fabricated for separation and detection of avian influenza viruses and pathogenic bacteria. Nanomaterials including magnetic nanoparticles, gold nanoparticles and quantum dots have been studied in immunomagnetic separation, DNA aptasensors, QCM immunosensors and fluorescent biosensors for rapid detection of pathogenic bacteria (E. coli O157:H7, Salmonella Typhimurium, Campylobacter jejuni and others) and viruses (avian influenza). Experiments have been conducted to prove the concepts of nanomaterials-enhanced biosensors and to characterize the performance of innovative biosensing methods such as detection sensitivity, specificity, repeatability and time. For Objective 2, nanofabrication, biosensing technology and risk assessment have been taught in undergraduate and graduate courses including Micro-Nanofabrication, Advanced Instrumentation and Biological Safety Engineering. Journal articles have been published in the areas of food safety, analytical chemistry, sensors and detection, and biological engineering. Research papers were presented in professional international meetings including ASABE, IAFP, ACS and regional meetings.

Publications

  • Type: Book Chapters Status: Published Year Published: 2017 Citation: 1) Li, Y., and R. Wang. 2017. Aptasensors for Detection of Avian Influenza Virus H5N1. P. 379-402, In: Methods in Molecular Biology  Biosensor and Biodetection, B. Prickril and A. Rasooly (ed). Springer, New York, NY
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Li, Z.M., Y.C. Fu, M. Liao, and Y. Li*. 2017. Biosensing methods for detection of highly pathogenic avian influenza H5N1 and H7N9 viruses. Analytical Methods 9(36):5238-5248. DOI: 10.1039/C7AY01585B
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Qiao, Z.H., C.Y. Lei, Y.C. Fu, and Y. Li*. 2017. An antimicrobial peptide-based colorimetric bioassay for rapid and sensitive detection of E. coli O157:H7. RSC Advances 7(26):15769-15775. doi:10.1039/c6ra28362d
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wang, L.J., R. Wang, F. Chen, T.S. Jiang, H. Wang, M.F. Slavik, H. Wei, and Y. Li*. 2017. QCM-based aptamer selection and detection of Salmonella Typhimurium. Food Chemistry 221:776-782. doi.org/10.1016/j.foodchem.2016.11.104
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wang, L.J., R. Wang, H. Wang, M.F. Slavik, H. Wei, and Y. Li*. 2017. An aptamer-based PCR method coupled with magnetic immunoseparation for sensitive detection of Salmonella Typhimurium in ground turkey. Analytical Biochemistry 533:34-40. doi.org/10.1016/j.ab.2017.06.010
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wang, R.H., L.J. Wang, Z. Callaway, H.G. Lu, T.J. Huang, and Y. Li*. 2017. A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus. Sensors an Actuators B: Chemical 240:934-940. doi.org/10.1016/j.snb.2016.09.067
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wen, T., R. Wang, A. Sotero, and Y. Li*. 2017. A portable impedance immunosensing system for rapid detection of Salmonella Typhimurium. Sensors. Sensors 17:1973. doi:10.3390/s17091973
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Xu, M., R. Wang, and Y. Li*. 2017. Electrochemical biosensors for rapid detection of Escherichia coli O157:H7- A review. Talanta 162:511-522. doi.org/10.1016/j.talanta.2016.10.050
  • Type: Journal Articles Status: Accepted Year Published: 2017 Citation: Yu, X.F., F. Chen, R.H. Wang, and Y. Li*. 2017. Whole-bacterium SELEX of DNA aptamers for rapid detection of E. coli O157:H7 using a QCM sensor. Journal of Biotechnology. (accepted on December 10, 2017)
  • Type: Journal Articles Status: Under Review Year Published: 2017 Citation: Callaway, Z., L.Z. Xu, R.H. Wang, and Y. Li*. 2016. Modeling of bacterial cells attached with magnetic nanoparticles in magnetic bioseparation. Transactions of the ASABE (submitted July 1, 2017; revised December 15, 2017)
  • Type: Journal Articles Status: Under Review Year Published: 2017 Citation: Wang, H., L.J. Wang, Q.Q. Hu, R.H. Wang, Y. Li* and M. Kidd. 2017. A nanoparticles-based QCM immunosensor for specific and sensitive detection of Campylobacter jejuni in poultry products. Journal of Food Protection (submitted Sept 3, 2017).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Li, Y. 2017. Biosensors for agriculture and food: Opportunities and challenge. An invited presentation at 1st International Forum on Smart Agriculture (SmartAg 2017), East Lansing, MI, December 3-6, 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Li, Y., J.H. Lin, J.P. Wang, and M. Liao. 2017. Nanomaterials-based biosensor systems for rapid detection of Salmonella Typhimusirum in poultry supply chain. An invited presentation at ACS 2017 Fall Conference, Washington, DC, August 20-24, 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Li, Y. 2017. Biosensors for agriculture and food: Opportunities and challenge. An invited presentation at 1st China Forum on Biosensors, Biochips and Nanotechnolgy (BBN China 2017), Foshan, Guangdong, China, October 19-21, 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Wang, R., M. Xu, J.H. Lin, M. Liao, M. Kidd, and Y. Li*. 2017. An electrochemical aptasensor for rapid detection of Salmonella Typhimurium in poultry based on the bifunctional magnetic nanocomposites. A poster presented at IAFP 2017 Annual Meeting, July 9-12, 2017, Tampa, FL. Paper No. P3-179.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Wen, T., R. Wang, A. Sotero, and Y. Li*. 2017. A portable impedance immunosensing system for rapid detection of Salmonella Typhimurium. ASABE Paper No. 1700368. An oral presentation at ASABE 2017 Annual International Meeting, July 16-19, 2017, Spokane, WA. Winner of ASABE-ITSC Meeting Paper Award.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Sotero, A., X.G. Xi, T. Wen, R. Wang, and Y. Li*. 2017. Rapid detection of Salmonella Typhimurium in poultry using a portable immunosensing system. A poster presented at AAFP 2017 Annual Meeting, September 19-21, 2017, Springdale, AR.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Yu, X.F., F. Chen, R. Wang, and Y. Li*. 2017. Selection of DNA aptamers using whole-bacterium SELEX for rapid detection of E. coli O157:H7 with a QCM sensor. A poster presented at AAFP 2017 Annual Meeting, September 19-21, 2017, Springdale, AR.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Xi, X.G., J.H. Lin, R. Wang, and Y. Li*. 2017. A fluorescent biosensor based on quantum dots, magnetic nanoparticles and microfluidics for in-field detection of foodborne pathogens. A poster presented at ASABE-Arkansas Section 2017 Annual Meeting, October 6, 2017, Fayetteville, AR.