Source: CORNELL UNIVERSITY submitted to NRP
DEVELOPING NEW SUCCESS METRICS FOR INVASIVE PLANT SPECIES MANAGEMENT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1010988
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Dec 13, 2016
Project End Date
Sep 30, 2019
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Natural Resources
Non Technical Summary
With numerous entry ports and extensive agriculture, forests, and waterbodies, New York State is particularly vulnerable to biological invasions and impacts they cause. Developing effective control methods requires research, yet decades of work by scientists and managers seeking solutions to invasive species problems continue to be plagued by (1) lack of understanding of invasive species impacts, and (2) lack of appropriate protocols to assess impacts. Furthermore, managers rarely assess impacts of management implemented to reduce negative effects of introduced species. This dilemma is created by a gap between knowledge and practice, where knowledge generated by research is difficult to translate into practice. Management is usually planned and conducted without goals of gaining knowledge from management practices. We consider the role of academia to deliver scientifically sound and standardized assessment protocols which, after field testing, can be utilized by land managers. Developing and implementing scientifically guided protocols to better assess invasive species management is a sorely needed. Ever increasing amounts of societal resources are invested to reduce negative impacts, yet we rarely receive evidence to understand whether management improves conditions for species, ecosystems or processes we aim to protect. The typical metric of how many hectares were treated or invasive species removed are invalid. Our protocols will not only contribute toinvasive species knowledge, but improve decision making and practice of invasive species management.
Animal Health Component
30%
Research Effort Categories
Basic
60%
Applied
30%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
12306201060100%
Goals / Objectives
Non-indigenous species, particularly many invasive plants that reach high local and regional abundance, are considered major ecological and agricultural problems (Mack et al., 2000) taxing the nations financial and intellectual resources. With numerous entry ports and extensive agriculture, forests, and water bodies, New York State is particularly vulnerable to biological invasions and impacts they may cause. However, whether these plant species are always the main drivers of environmental degradation is contested (MacDougall and Turkington, 2005; Davis et al., 2011) questioning the wisdom of the ever increasing expenditures. For example, invasion success of some introduced plants is linked to introduced earthworms (Nuzzo et al., 2009) and high native or introduced ungulate (deer, sheep, bovid) populations (Kalisz et al., 2014), both of which have distinct ecosystem impacts (Bohlen et al., 2004; Côté et al., 2004). Annual herbicide expenditures for a single wetland invader, Phragmites australis, exceed $4 million (pre 2009 data) with little or no long-term benefit as self-reported by managers (Martin and Blossey, 2013). Despite these sobering assessments, the Great Lakes Restoration Initiative (GLRI) alone has provided >$20 million since 2011 for continued P. australis herbicide treatments (https://www.glri.us//projects/index.html).Developing effective control methods requires research, yet decades of work by scientists and managers seeking solutions to invasive species problems continue to be plagued by (1) lack of understanding of invasive species impacts, and (2) lack of appropriate protocols to assess impacts. Furthermore, managers rarely assess impacts of management implemented to reduce negative effects of introduced species. Case in point are the GLRI P. australis projects. Unfortunately, there is no required follow-up to evaluate whether treatments made life better for the species considered under threat by P. australis invasion. Evidence from one of our collaborators in the Adirondack PRISM (Quirion et al., MS in preparation), suggests that while P. australis populations can be suppressed with annual herbicide treatments, eradication is only possible when extremely small populations (10x10 m or less) are targeted. Any benefits to native species and unwarranted potential side effects of continual herbicide application are entirely unknown as only anecdotal observations are typically collected. The lack of assessments of ecological outcomes of control efforts is not just a problem in P. australis management, but it has plagued invasive species management since its inception (Blossey, 1999; Downey, 2011).Ever increasing amounts of societal resources are invested to reduce negative impacts of invasive plants, yet we rarely receive evidence to understand whether management improves conditions for species, ecosystems or processes we aim to protect. The typical metrics of how many hectares were treated or the numbers of individuals of an invasive species removed are invalid. This dilemma is created by a gap between knowledge and practice, where knowledge generated by research is difficult to translate into practice (Fabricius and Cundill, 2014). Furthermore, the selected management techniques may not be able to successfully achieve the desired ecological outcomes, but that is rarely assessed. In addition, management is usually planned and conducted without goals of gaining knowledge from management practices.We consider the role of academia to deliver scientifically sound and standardized assessment protocols, which - after field-testing - can be utilized by land managers. Developing and implementing scientifically guided protocols to better assess invasive species management is sorely needed but academics have not embraced this social responsibility (Fabricius and Cundill, 2014) and left it to the managers to design and conduct assessments. Managers have often rejected the need for assessments for financial and time constraints, but that appears more a function of institutional cultures (Smith, 2011), as well as the lack of scientific expertise by those charged with implementing control efforts. Our proposed work program and direct engagement (for example through a structured decision making process funded through the NY Invasive Species Research Institute) with managers will not only contribute to invasive species knowledge, but improve decision making and practice of invasive species management.Our work program touches aspects of nearly all important articulated extension and research priorities for NYS (http://www2.cce.cornell.edu/plans/Pages/FY-2016-CCE-Programmatic-Plans.aspx) with the exception of 4.0 Nutrition, Food Safety and Security and 5.0 4-H. But even these two programs, particularly food security and 4-H can overlap with our work if invasive species threats are removed so forest stewardship and agro-forestry can be developed/maintained and with 4-H involved in local stewardship, such as management of invasive plants through physical removal and restoration. But most formidable, our work program overlaps with goals articulated in the remaining priorities such as Agriculture and Food Systems (increase the use of sustainable practices to result in improved or protected soil, air and water quality and production of high quality and safe food and fiber), Climate Change (management of invasive species using IPM or otherwise), Environment and Natural Resources (natural resource conservation as a general theme), and Community and Economic Vitality. Our program aims to grow and develop "community leadership capacity so that community residents experience high quality of life, ecological integrity, effective decision making". With this leadership in place communities should be enable to better actively manage their human and environmental assets.
Project Methods
Objective 1: Negative impacts of invasive plants are often measured in response to removal of invaders. However, this approach does not account for associated confounding factors, (vegetation differences, legacy effects, and interactions with deer, earthworms, and other invasive species). We will use transplants of native plant species (sentinels) with conservation relevance to assess invasive plant impacts. Only if species can be made readily available do they have utility for the managers. We have adopted such an approachsuccessfully to assess deer impacts using red oak sentinels. The sentinel approach allows rapid impact assessments. Furthermore, the method does not require specialized botanical knowledge facilitating adoption by land managers. Specimens are individually numbered and will be revisitedat regular intervals.We identified 3 target invasive plants: Alliaria petiolata (garlic mustard), Cynanchum rossicum (pale swallow-wort) and Fallopia bohemica (Bohemian knotweed; this hybrid of F. japonica and F. sacchalinensis is the most widespread form of a complex known as Japanese knotweeds in our region (unoublished data). All three plant species are present throughout the Finger Lakes region and are commonly targeted for control.For A. petiolata and C. rossicum we will select 10 invaded sites per plant species. Within each site we will select 2 study areas, a control and an area where management will occur. We will focus on invasions occurring in forested areas or along edges between open fields and forests. Both species are removed through mechanical methods (pulling for A. petiolata and digging for C. rossicum). We will partner with local management agencies to coordinate plant removal efforts. Removal will occur annually for the duration of the study.White-tailed deerbenefitinvasive plant species, including A. petiolata (Knight et al., 2009; Kalisz et al., 2014; Davalos et al., 2015). Therefore, proper assessments of invasive species impacts requires untangling single and combined effects. We will evaluate deer impacts by dividing each study area into two plots: one open where deer have access and one fenced plot where deer are excluded. We will follow a split-plot design where each site is divided into two management areas (control and removal) and each area into two deer access treatments (open and fenced, each anticipated to be 10x10m but this may vary according to local conditions), for a total of four study plots per site.Non-native earthworms are often correlated with invasive plants (Nuzzo et al. 2009) and white-tailed deer presence (Davalos et al. 2015). Furthermore, earthworms have significant effects on plant communities (Frelich et al., 2006; Dobson and Blossey, 2015). To avoid confounding effects of site conditions we will only select sites where earthworms are present. At least for A. petiolata, it appears earthworm invasions occur first and A. petiolata is taking advantage of soil conditions created by earthworms (Nuzzo et al. 2009). We will determine earthworm presence through earthworm extraction with the mustard method (Lawrence and Bowers, 2002) and observation of earthworms casts. We will transplant multiple seedlings (N=20) of sentinel plant species at each plot and monitor their survival, growth and reproductive output throughout the duration of the study (N=800 sentinels per species; 20 sentinels per plot x 4 plots per site x 10 sites). We will make an initial selection of species based on desiredplant communities land managers like to achieve. From this list, we will select 3-5 sentinel species based on their occurrence in the target habitat, species life history (annual vs. perennial), life form (graminoid, forb, woody, etc.), deer preference and ease to propagate or purchase seedlings. Growth and reproductive measurements may include stem height, leaf number, leaf width or length, flower or fruit number. Selected measurements will vary among sentinel species depending on growth habits. We will evaluate single and combined effect of each factor (invasive species removal and deer exclusion) via linear mixed models where site and area within site will be included as random factors.We will evaluate benefits of F. bohemica management (herbicide application; both stem and foliar application) for native vegetation using the sentinel approach as described above (sentinel species may differ among sites based on articulated desired plant communities by land managers) . We will conduct the study at a network of sites managed by Cornell Plantations and other local land owners. The sites differ in area covered by F. bohemica, years of treatment, vegetation community, and current status of invasion, allowing us to also evaluate temporal and spatial effects. For example we will be able to address the question whether potential negative effects of herbicide application linger or dissipate over time.Objective 2: To overcome gaps between invasive species research and practice, we seek to couple knowledge generation and management. Once the protocol described in Objective 1 is field tested, we will work with land managers to develop a learning process and decision framework for invasive species management. We will have set the stage for this process through an independently funded project that will use structured decision-making processes with NY PRISM leaders. We will utilize the outcome or intermittent results of this multi-year process to further develop our own approach. Based on "action learning theory", we will cultivate collaboration between researchers and managers in an "action learning cycle". The cycle includes: Planning (setting invasive species management objectives and selecting the appropriate control method to reach those objectives, i.e structured decision making framework), Action (implementing control strategy), Evaluating (assessing results), and Reflecting (identifying successes and ways to improve). While the essential components of this approach resemble 'adaptive management', we deliberately call it "action learning" as we are seeking scientist-manager collaboration and local knowledge generation and learning as a main outcome. Adaptive management, in practice, has often failed the complete the ideal iterative process due to a lack of true collaborative efforts and development and implementation of assessments.Managers typically only do the first two steps (planning and action), therefore failing to gain local information about invasive species impacts and management success that come from steps three and four (evaluating and reflecting) (Armitage et al., 2009; Fabricius and Cundill, 2014). Likewise, researchers testing invasive species control strategies infrequently report on whether native plant populations reestablish and seldom monitor post treatment success over appropriate timeframes or scales (Kettenring and Adams, 2011)We will start to employ decision-making processes to clearly set management objectives and weigh the cost- benefits of management options to meet those objectives using tools including SDM (Gregory et al., 2012) and the Invasive Plant Management Decision Analysis Tool (Zimmerman et al., 2011)i. This will be a collaborative effort guided by SDM specialists and involve scientists and our partners in NY PRISMS.Through this partnership, we will build a framework of collaboration where managers and researchers jointly define objectives and use scientifically valid metrics to measure impacts and outcomes of management at real management scales and within appropriate timeframes. The framework will include (1) a process to articulate attainable management objectives and appropriate metrics to measure success (largely defined through the SDM process, (2) a technical approach to plan management activities such that scientifically sound data can be collected and (3) analysis, reflection and communication of results.

Progress 10/01/18 to 09/30/19

Outputs
Target Audience:We have continued to engage with the New York Partnership for Regional Invasive Species Management (PRISM) network through individual meetings with staff at the Adirondack Chapter of The Nature Conservancy and active involvement in the Great Lakes Slender False Brome Group of the Western New York PRISM. Changes/Problems:As detailed in the previous report, we encounteretd delays in implementing some of your field work. 2019 allowed us to catch up, but we did not accomplish our goals of outreach to those wanting to use similar methods. We have been granted an extension and plan to accomplish these last pieces, pus an aditional year of monitoring for survivaland growth of indicator plants in 2020 What opportunities for training and professional development has the project provided?Audrey Bowe continues to pursue her MS degree through the employee degree program at Cornell University overseeing much of the work in this project. This project has allowed 6 undergraduate research assistants at Cornell University to learn about and participate in native plant identification, propagation and maintenance, and has led to the development of one undergraduate honors thesis project led by Abigail Bezrutczyk. Individuals of propagated sentinel plants were also used in outreach events conveying the importance of our native plants including a Fern Identification Workshop as part of a weekend-long Master Naturalist Training held at the Arnot Forest in Van Etten, New York in September 2019. Propagated plants were also shared with a researcher at SUNY Cortland engaged in a related project working with swallow-wort management New York State Parks. How have the results been disseminated to communities of interest?Preliminary results from the Brachypodium sentinel project were presented at the 2019 North American Invasive Species Management Association Conference in Saratoga Springs, New York in September to an audience of invasive species managers and researchers. Results and future plans were also shared at the November meeting of the Great Lakes Slender False Brome Working Group in Genesee County, New York. Working with the New York Invasive Species Research Institute, a workshop on Metrics of Success is planned for early 2020 where we will engage with land managers and researchers on topics of establishing appropriate metrics and monitoring in invasive species management. What do you plan to do during the next reporting period to accomplish the goals?In the coming field season, we plan to follow plant survival and growth for both (Adirondack and Finger Lakes) projects. For both projects, we also plan to measure important site characteristics at field sites, including plant community composition, soil attributes, earthworm communities, and aspects of hydrology. Once we have these measurements, we plan to analyze our 2 years of data and write up a formal reportof our findings, as well as a paper describing the indicator approach method. We will also present final findings to the Great Lakes Slender False Brome Working Group as well as share them with collaborators at the Adirondack Park Invasive Plant Program. We are also currently in conversation with land managers in Western New York about how the sentinel approach might be incorporated into some of their Brachypodium management sites.

Impacts
What was accomplished under these goals? In 2019, we established field sites in the Adirondack Park (4 sites) and the Finger Lakes Region (3 sites). In the Finger Lakes Region, deer fences and other infrastructure were established in the Brachypodium study area in May. In June, six species of experimental plants (Eurybia divaricata, Polystichum acrostichoides, Viburnum acerifolium, Solidago caesia, Quercus rubra, and Elymus histrix) were measured and planted into sites. Plants were surveyed for survival after 2 weeks and dead individuals were replaced to account for any transplant shock. In August, we revisited sites and assessed survival and growth of all plants. In the Adirondack Region, we used historic Phragmites management records to determine planting locations using ArcGIS in June 2019. In late July, planting markers were established at four wetland sites. A fifth site was excluded due to extensive flooding. In early August, three species (Onoclea sensibilis, Spiraea alba, and Asclepias incarnata). We assessed 2-week survival and replaced individuals as needed and took final plant survival and growth measurements in mid-September.

Publications

  • Type: Journal Articles Status: Accepted Year Published: 2020 Citation: Impacts of Invasive Earthworms and Deer on Native Ferns in Forests of Northeastern North America


Progress 12/13/16 to 09/30/19

Outputs
Target Audience:We have engaged with the New York (NY) Partnership for Regional Invasive Species Management (PRISM) network through individual meetings with staff at the Adirondack Chapter of The Nature Conservancy and active involvement in the Great Lakes Slender False Brome Group of the Western NY PRISM. In 2020 these meetings were held all virtually. Changes/Problems:There are no major problems to report that are of scientific nature. We have reported that we faced delays in obtaining permits to work in the Adirondack Park, and then Covid-19 related restriction prevented us from hosting the anticipated in-person workshops to teach land managers the use of the sentinel methods we developed. Other resources and the network of the New York Invasive Species Research Institute will enable us to proceed with these efforts. What opportunities for training and professional development has the project provided?Audrey Bowe will complete her MS degree through the employee degree program at Cornell University having overseenthe work in this project. This project has also allowed 6 undergraduate research assistants at Cornell University to learn about and participate in native plant identification, propagation and maintenance, and has led to the development of one undergraduate honors thesis project on negative soil feedback led by Abby Bezrutczyk. Abby is currently writing up these results for a publication to be submitted in spring 2021. Individuals of propagated sentinel plants were also used in outreach events conveying the importance of our native plants including a Fern Identification Workshop as part of a weekend-long Master Naturalist Training held at the Arnot Forest in Van Etten, NY in September 2019. Propagated plants were also shared with a researcher at SUNY Cortland engaged in a related project working with swallow-wort management New York State Parks. How have the results been disseminated to communities of interest?Throughout the performance period, we have advanced ideas regarding the use of sentinel plantings in many different in-person and online presentations. For example, results from the Brachypodium sentinel project were presented at the 2019 North American Invasive Species Management Association Conference in Saratoga Springs, NY in September to an audience of invasive species managers and researchers. Results and future plans were also shared at the November 2019 meeting of the Great Lakes Slender False Brome Working Group in Genesee County, NY. Working with the New York Invasive Species Research Institute, a workshop on Metrics Success is planned for early 2021where we will engage with land managers and researchers on topics of establishing appropriate metrics and monitoring in invasive species management. This will be done initially virtually and then we will follow up with an in-person training once this can be completed safely. We are planning multiple scientific publications of the core results of the transplant studies, the assessment of negative soil feedback, plus a "white paper" on the use of sentinel plants. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? In 2019, we established field research sites in the Adirondack Park (4 sites for Phragmites,Phragmites australis australis) and the Finger Lakes Region (3 sites, slender false broom, Brachypodium sylvaticum, work). In the Finger Lakes Region, deer fences and other infrastructure were established in the Brachypodium study area in May. In June, six species of experimental plants (Eurybia divaricata, Polystichum acrostichoides, Viburnum acerifolium, Solidago caesia, Quercus rubra, and Elymus histrix) were measured and planted into sites. Plants were surveyed for survival after 2 weeks and dead individuals were replaced to account for any transplant shock. In August, we revisited sites and assessed survival and growth of all plants. We were able to repeat these measurements despite Covid-19 work restrictions in 2020. The major findings, although data are still being analyzed, is that we saw a decline in survival for some species when grown in the presence of slender flase brom. We are currently exploring the mechanisms, in particular whether this is a function of simply increased cover and plant-plant competition, or potential allopathic impacts. We will be using the recorded data on cover (%) in our analyses and we know that negative soil feedback does not play a role due to an undergraduate honors project (also see section on opportunities for professional development). In the honor project, we explored whether presence of negative soil feedback on Brachypodium affects growth and survival of 10 different native and introduced plant species. We found no evidence for negative soil feedback in that study. In the Adirondack Region, we used historic Phragmites management records to determine planting locations using ArcGIS in June 2019. In late July, planting markers were established at four wetland sites. A fifth site was excluded due to extensive flooding. In early August, we planted propagated young plants of three species (Onoclea sensibilis, Spiraea alba, and Asclepias incarnata). We assessed 2-week survival and replaced individuals as needed and took final plant survival and growth measurements in mid-September. We repeated survival and growth measurements in 2020. As for slender false broom, the major impacts we recorded was on survival (and not growth or reproduction) of our transplant species. In areas previously treated with herbicide, survival of all species was significantly reduced compared to areas that remained untreated, but also did not have a Phragmites presence. Our results indicate either a long-term detrimental effect of Phragmites invasion on native plant species survival, or, more likely, a poorly recognized long-term impact of management using herbicide. We will need follow-up work to determine the true mechanisms, but also explore whether repeat treatments increase mortality rates, which would indicate herbicide impacts. Negative herbicide impactshavebeen documented in other systems, but are poorly recognized or evaluated. Our indicator approach has shown great potential to be used as an assessment tool, both to measure impact of introduced plant species, or of management efforts. By planting focal individuals we no longer need to rely on existing members of a plant community, and we are able to exclude other potential confounding factors (such as deer or earthworms). Unfortunately, Covid-19 prevented us from hosting the anticipated workshops to tech land managers in this technique. However, we are advancing virtual learning opportunities through the NY Invasive Species Research Institute in 2021, and we will host in-person workshops to promote this technique once safely possible.

Publications


    Progress 10/01/17 to 09/30/18

    Outputs
    Target Audience:We have continued to work with the NY Partnership for Regional Invasive Species Management (PRISM) network through individual meetings with staff at the Adirondack Chapter of The Nature Conservancy and active involvement in the Great Lakes Slender False Brome Group of the Western New York PRISM. In addition, we have started to collaborate with the Bureau of Forestry within the New York State Department of Environmental Conservation and explored work with the Bureau of Wildlife. Changes/Problems:We shifted plantings of seedlings to spring 2019 due to delays in receiving work permits at our selected sites, and due to the desire to plant well-established individuals at our research locations. We now have these individuals in place. It will not have impact on expenditures or findings. Our goal was always to establish a long-term project and to assess feasibility of the approach. We will still be able to accomplish this in the time remaining in the project. What opportunities for training and professional development has the project provided?Audrey Bowe continues to pursue her MS degree through the employee degree program at Cornell University overseeing much of the work in this project. In addition, this project has allowed several undergraduates at Cornell University to learn about and participate in native plant identification, propagation and maintenance, and may lead to further opportunities in the form of undergraduate research projects. Individuals of propagated sentinel plants were used in several outreach events conveying the importance of our native plants including Judy's Day of the Cornell Botanic Gardens in Ithaca, NY, and a Fern Identification Workshop as part of a weekend-long Master Naturalist Training held at the Arnot Forest in Van Etten, NY. Propagated plants were also shared with a researcher at SUNY Cortland engaged in a related project working with New York State Parks. How have the results been disseminated to communities of interest?It is too early in the project to disseminate results. What has been more important is to involve management agencies, land stewards and those interested in control of invasive species in the approach (both in general terms and in the details), planning and execution of our proposed research. This has been very important in getting "buy-in" from those offering research locations, or for those interested in developing a regional control for one of our target species (B. sylvaticum), which has just recently been detected in New York State. These efforts using direct contacts, joint field visits, and presentations at workshops will continue. What do you plan to do during the next reporting period to accomplish the goals?We shifted plantings of seedlings to spring 2019 due to delays in receiving work permits at our selected sites, and due to the desire to plant well-established individuals at our research locations. We now have these individuals in place. In 2019, field site infrastructure (fences, transects, plant labelling etc.) both in the Adirondacks (5 sites), and in the Finger Lakes Region (3 sites) will be established and individuals planted and monitored. We will involve seasonal staff of The Adirondack Chapter of The Nature Conservancy in the implementation, and plan to report back results to the Great Lakes Slender False Brome Working Group. The fall will see a summary of the data we collected and we can provide an annual summary of seedling survival and how previous or ongoing management may have affected the outcomes.

    Impacts
    What was accomplished under these goals? In early 2018, we worked extensively with staff at the Adirondack Chapter of The Nature Conservancy to understand their experience with management of Phragmites australis, and co-create a research project that would work within the context of available research sites, data, as well as the current management regime. Through discussions, digital exploration, and field visits, we narrowed down a list of field sites to identify 6 appropriate sites in the Adirondacks and 3 sites in the Finger Lakes Region. We applied for and received the appropriate permissions for using selected field sites on New York Department of Environmental Conservation land in late September 2018. We also presented and discussed our proposed project at the fall quarterly meeting of the Great Lakes Slender False Brome Working Group, held at Genesee County Park & Forest in East Bethany, NY. Upon request, we compiled and shared a database of Brachypodium sylvaticum research with those managing this invasive grass. Sentinel plants that had been propagated in 2017 were maintained and further local seed and spores were collected and processed as needed.

    Publications


      Progress 12/13/16 to 09/30/17

      Outputs
      Target Audience:We have worked with land managers in various New York Invasive Species Management Units (PRISMS), particularly in the Finger Lakes Region and in the Adirondacks, to select appropriate field sites. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Audrey Bowe, a recent undergraduate in the Department of Natural Resources, has taken the lead on the project. She has entered the employee degree program at Cornell University to obtain a MS. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Major efforts will be made to propagate plants and establish field locations, followed by monitoring of establishment, survival and growth of indicators.

      Impacts
      What was accomplished under these goals? We have extensively surveyed for appropriate field sites, target invasive species, and sentinel species (plants). We have obtained verbal approval to work at the selected locations, we have discussed our plans with the land managers in charge of these areas, and collected plant material (seeds) for germination over the winter and spring.

      Publications