Source: MICHIGAN TECHNOLOGICAL UNIV submitted to NRP
REGULATION OF BIOMASS GROWTH IN POPLAR UNDER NITROGEN AND WATER DEPRIVATION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
MCINTIRE-STENNIS
Reporting Frequency
Annual
Accession No.
1010961
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 24, 2016
Project End Date
Sep 30, 2020
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
MICHIGAN TECHNOLOGICAL UNIV
1400 Townsend Drive
HOUGHTON,MI 49931
Performing Department
College of Forest Resources and Environmental Science
Non Technical Summary
This project aims to characterize several poplar genes in relation to their effect on biomass growth under nitrogen- and water-limiting conditions. Knowledge attained through this project can lead to development of bioenergy varieties that can produce lignocellulosic biomass with minimal or no input.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20306701040100%
Knowledge Area
203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants;

Subject Of Investigation
0670 - Short rotation woody crops, including holiday trees;

Field Of Science
1040 - Molecular biology;
Goals / Objectives
The major goal of the project is to identify genes that can improve nitrogen-and water-use efficiency in poplar for growth on marginal lands with little or no input. The objectives of the proposal are to:Perform detailed phenotypic characterization of the transgenic plants with modified expression of the genes under study in relation to the traits of interest (e.g., nitrogen- and water-use efficiency).Study the expression and localization of these genes in relation to the tissues and the physiological and stress conditions of interest to better understand their function and mechanism of action; andPerform physiological, transcriptomics, and biochemical analyses of the transgenic plants to draw mechanistic inferences about their mode of action.
Project Methods
We will use physiological, biochemical and molecular biology methods to charcaterize the genes and the transgenic plants.

Progress 10/24/16 to 09/30/20

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Peer reviewed publications. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The goals of this projects were acomplished and were published in peer reviewed publications acknowledging the support of the grant.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Rauschendorfer J, Yordanov Y*, Dobrev P, Vankova R, Sykes R, Kulheim C, Busov V (2020) Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation. Plant Biotechnology Journal 18 (6):1434-1443


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Peer scientists;tree breeders; environmental scientists and practioners; foresters; horticulturists Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Scientist worked on the project and was exposed to various techniques and experimental procedures. How have the results been disseminated to communities of interest?Peer reviewer papers. What do you plan to do during the next reporting period to accomplish the goals?Continue working on all three goals of the project.

Impacts
What was accomplished under these goals? Progress was made along all three goals and the results are being prepared for publication.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Busov, V.B. (2018). Manipulation of Growth and Architectural Characteristics in Trees for Increased Woody Biomass Production. Frontiers in Plant Science 9 (1505). doi: 10.3389/fpls.2018.01505.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Peer scientists;tree breeders; environmental scientists and practioners; foresters; horticulturists Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Scientist worked on the project and was exposed to various techniques and experimental procedures. How have the results been disseminated to communities of interest?Peer reviewer papers. What do you plan to do during the next reporting period to accomplish the goals?Continue working on all three goals of the project.

Impacts
What was accomplished under these goals? Progress was made along all three goals and the results were published. See previous 'Product' section.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Dash, M., Yordanov*, Y. S., Georgieva*, T., Wei, H. Busov, V. B. (2018) Gene network analysis of poplar root transcriptome in response to drought stress identifies a PtaJAZ3 PtaRAP2.6-centered hierarchical network. doi.org/10.1371/journal.pone.0208560
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Busov, V.B. (2018). Manipulation of Growth and Architectural Characteristics in Trees for Increased Woody Biomass Production. Frontiers in Plant Science 9 (1505). doi: 10.3389/fpls.2018.01505.


Progress 10/24/16 to 09/30/17

Outputs
Target Audience:Peer scientists;tree breeders; environmental scientists and practioners; foresters; horticulturists Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Scientist and postdoctoral scientsist worked on th eproject and were exposed to various techniques and experimental procedures. Postdoctoral scientist helped in writing the papers. How have the results been disseminated to communities of interest?Peer reviewer papers. What do you plan to do during the next reporting period to accomplish the goals?Continue working on all three goals of the project.

Impacts
What was accomplished under these goals? Progress was made along all three goals and the results were published. See previous 'Product' section.

Publications

  • Type: Other Status: Published Year Published: 2017 Citation: Yordanov YS, Ma C, Yordanova E, Meilan R, Strauss SH, Busov VB.(2017) BIG LEAF is a regulator of organ size and adventitious root formation in poplar. PLoS One. 2017 Feb 3;12(2):e0171532. doi: 10.1371/journal.pone.0171532.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Dash M, Yordanov YS.(2016)A network of genes associated with poplar root development in response to low nitrogen. Georgieva T, Kumari S, Wei H, Busov V. Plant Signal Behav. 2016 Aug 2;11(8):e1214792. doi: 10.1080/15592324.2016.1214792.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Dash M, Yordanov YS, Georgieva T, Tschaplinski TJ, Yordanova E, Busov V.(2017) Poplar PtabZIP1-like enhances lateral root formation and biomass growth under drought stress. Plant J. 2017 Feb;89(4):692-705. doi: 10.1111/tpj.13413.