Source: MICHIGAN STATE UNIV submitted to
NANOTECHNOLOGY AND BIOSENSORS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1010683
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
NC-_old1194
Project Start Date
Oct 1, 2016
Project End Date
Sep 30, 2021
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Project Director
Alocilja, EV.
Recipient Organization
MICHIGAN STATE UNIV
(N/A)
EAST LANSING,MI 48824
Performing Department
Biosystems & Agric Engineering
Non Technical Summary
Development of sensing and processing technologies utilizing nanotechnology has become more relevant for furthering our understanding of biological phenomena related to food, agriculture, environment, and energy. As we understand the molecular mechanisms that underlie the emergence and spread of pathogens and their consequent impact on our agricultural and food systems, and gain insight into the molecular mechanism of life itself, it becomes clear that the technology to investigate, intervene and mitigate need to be correspondingly small, i.e. within the realm of nanotechnology.According to the FY 2014-2018 Strategic Plan published by the USDA, one of the strategic goals for this period is to "Ensure that all of America's children have access to safe, nutritious and balanced meals." Within this goal, Specific Objective 4.3 states that we must "Protect public health by ensuring that food is safe". Reduction in the number of foodborne illnesses will require a comprehensive "farm-to-fork" risk assessment, coupled with appropriate technological measures to screen, identify, and eliminate offending food pathogens.In addition to safety concerns in processed foods, plant/animal pathogens also represent major threats to worldwide food security. Rapid responses to crops and livestock epidemics require fast and accurate evaluation of plant/animal pathogens, which also calls for advances in biosensor technology to provide more user-friendly tools and techniques.Besides pathogen detection, novel biosensors could support better and more rapid monitoring of crop growth and animal welfare, which could lead to better practice of production/processing that will help safeguard both food security and environmental sustainability in the long term.
Animal Health Component
10%
Research Effort Categories
Basic
45%
Applied
10%
Developmental
45%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
40250102020100%
Knowledge Area
402 - Engineering Systems and Equipment;

Subject Of Investigation
5010 - Food;

Field Of Science
2020 - Engineering;
Goals / Objectives
Develop devices and systems incorporating microfabrication and nanotechnology
Project Methods
OBJECTIVE 3: Develop biological interfaces and sensing systems incorporating microfabrication and nanotechnologya) Development of nano-transducers and bio-recognition systemsResearch activities will revolve around the development of nanoparticles, nanowires, nanobars, and nano-pillars. Specific activities include i) Design and development of electrically active magnetic nanoparticles and gold nanoparticles with uniformly structured size and properties (electrical and magnetic) as well as green-synthesis approaches; ii) Development of controlled interfacing techniques for biological (such as DNA, proteins and antibodies) and abiological materials (such as carbon nanotubes and nanoparticles); iii) Biofunctionalization of composite nanoparticles and materials with multiple functionalities for the development of DNA and protein sensors; iv) Conversion of existing fluorescence and chem/bioluminescence-based sensing recognition solution schemes into nanopatternable solid state materials; v) Identification of receptor molecules for the fabrication of biosensor; vi) Synthesis of metaloxide nano-patterned substrate for biosensor applications; vii) Use of antibody-DNA, aptamer-DNA and other DNA conjugates for production of a uniform RNA/DNA signal correlated to biological recognition of proteins, whole cells and DNA/RNA through bio-nano-transduction; and viii) Development of nano-structured materials to be used as bio-mimetic transducers representing mammalian sensing capabilities (olfactory sensing, taste, touch etc.)b) Device designFocus is on the design and fabrication of novel biosensor platforms using conventional and state-of-the-art fabrication facilities. Specific activities include i) Fabrication of multi-array channels for simultaneous detection in one sample application using electrochemical and optical signal measurement; ii) Fabrication of deep devices in glass and plastic for cell transport and recovery as well as micro and nano-optical arrays for non-labeled sensing; iii) Development of nano and micro array patterned materials for fluorescent and luminescent based chemical and biological sensing of trace contaminants, toxins, and biological signaling molecules; iv) Development of smartphone-based biosensing device towards field-deployable immunoassay and PCR devices; v) System design and protocol development; vi) Fabrication of devices incorporating field effect transistors for detection of molecular charge; vii) Fabrication of MEMS-based components to be integrated with nano-scale systems/devices; and viii) Use of computational simulation and modeling techniques for rapid development and evaluation of MEMS/nano-scale devices.

Progress 10/01/16 to 09/30/21

Outputs
Target Audience:The various target audiences reached by our efforts during this reporting period were undergraduate students and graduatestudents at MSU, high school students, parents and family members of students, faculty and students from otheruniversities, industry personnel, government personnel, extension personnel,scientists, and the public. We have also reached manystudents, faculty members, and scientists from institutions of higher education in the Philippines, Japan, Peru, Mexico, Dominican Republic, Nepal, India, Pakistan, Kenya, Cameroon, South Africa, and the United Kingdom. Additionally, the 2021 Global Alliance for Rapid Diagnostics Forum, which we hosted virtually on June 25-26, 2021, reached 214 participants from around the world. Furthermore, our lab participated in the MSU Science Festival, held virtually throughout the month of April 2021. The goal of the MSU Science Festival is to celebrate the many ways science, technology, engineering, arts and mathematics, or STEAM, affectour everyday lives.We joined withover 75 MSU partners and 50 community partners, and the program's outreach stretched to 283 cities, 24 states and nine countries. Our lab also participated in the MSUPathfinders Program, which is an online summer program designed to help participants explore the fields of agriculture and natural resources. The program brings together MSU faculty and staff to lead students to a greater understanding of different careers and educational pathways. It is designed for students entering grades 9-12 who are willing to explore educational and career opportunities in environmental-, agricultural- and natural resource-focused areas. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained 7 undergraduate students, 1 MS student, and 4 PhD students. These students and scientists will become the future research leaders in the emerging field of nanotechnology-based biosensors forglobal health, biodefense, food safety, water quality, and product integrity. We have also reached thousands of students from around the world through the 2021 Global Alliance for Rapid Diagnostics Forum which was held on June 25-26, 2021, through our participation in the MSU Science Festival which was held on April 1-30, 2021,and through our participation in the MSU-CANR Pathfinders which was held on July 12-30, 2021. How have the results been disseminated to communities of interest?Research results have been disseminated through journal publications and conference presentations as well as through interactions with industry personnel. In addition, we continue to strengthen our global collaborationand networking in the Philippines, Japan, Nepal, India, Peru, Mexico, Dominican Republic, Kenya, Cameroon, South Africa, and the United Kingdom. What do you plan to do during the next reporting period to accomplish the goals?This is a terminal report for 2016-2021. We will implement the renewed NC-1194 for 2021-2026.

Impacts
What was accomplished under these goals? Our major accomplishments for the reporting period can be summarized as follows: 1. We continue to be the national and international leader in the synthesis, characterization, and functionalization ofmagnetic nanoparticles (MNPs) and thiolated gold nanoparticles (GNPs) forrapid, simple, field-operable,and affordable means to extract, isolate, concentrate, anddetect pathogens in complex matrices. 2. We have demonstrated that our MNPs and GNPs can extract and detect pathogens, respectively, at very low levels of contamination, leading to high sensitivity and high specificity performance of the nano-biosensor technologies. Wehave developed nano-biosensors for detecting E. coli, E. coli O157:H7, Salmonella Enteritidis, Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes,Mycobacterium tuberculosis, dengue, Japanese encephalitis, and SARS-CoV-2. We have validated them in various complex matrices (food, water, and clinical samples). 3. The MNPs and GNPs have been shared to support the research programs of collaborators around the world withhigh potential life-saving impacts in many resource-limited communities. 4. We have published numerous multi-authored peer-reviewed journalarticles and numerous technical presentations at professional conferences. 5. Through the many collaborative research programs facilitated by the nanoparticles (MNPs and GNPs) and biosensors, we have developed a manpower capacity program for many collaborating institutions which contribute to the overall capacity building of the nations.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Sharief, S. and Alocilja, E.C. 2021. Rapid and Direct Identification of Pathogens from Food Matrices Using Magnetic Nanoparticles and Machine Learning Algorithms Applied to High Resolution Microscope Images, International Association of Food Protection, Phoenix, AZ, July 18-21, 2021 (in-person meeting).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Sharief, S., Dester, E. Alocilja, E.C. 2021. Rapid and direct identification of pathogens from food matrices using magnetic nanoparticles and supervised machine learning algorithms applied to near infrared spectroscopy data, 2021 ASABE Annual Meeting, Virtual, July 12-16, 2021
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Alocilja, E. 2020. Nano-enabled Biosensor for Rapid Screening, Detection, and Monitoring of Infectious Diseases, Department of Chemistry and Biochemistry, Kettering University, December 10, 2020. (Invited presentation)
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Alocilja, E.C. 2021. Sustainable Food Through Nano-enabled Biosensors, National Nanotechnology Initiative Public Webinar, June 2, 2021. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Alocilja, E.C. 2021. Systems Framework for Personalized Infectious Disease Management Using Nano-enabled Biosensors: A Tool for Pandemic Control, National Systems Conference, sponsored by the Systems Society of India, May 22-23, 2021. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Oznur Caliskan-Aydogan, Evangelyn Alocija, 2021. Isolation and Binding Properties of Imipenem Exposed Salmonella Typhimurium with Magnetic Nanoparticles, ASABE Annual International Meeting, Virtual, July 12-16, 2021
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Caliskan-Aydogan, O. and Alocilja, E.C. 2021. Binding Properties of Imipenem Exposed Salmonella Typhimurium Using Glycan coated Magnetic Nanoparticles, Engineering Graduate Research Symposium (EGRS), Michigan State University - Virtual, May 5-12, 2021
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Sharief, S., Dester, E. and Alocilja, E.C. 2021. Rapid Classification of Foodborne Pathogens using Near Infrared Spectroscopy, Engineering Graduate Research Symposium (EGRS), Michigan State University - Virtual, May 5-12, 2021
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Billups, S., Sharief, S., and Alocilja, E.C. 2021. Rapid isolation and classification of pathogens using Magnetic Nanoparticles and Machine Learning, Mid-Michigan Symposium for Undergraduate Research Experiences (Mid-SURE), Michigan State University, Virtual, July 28, 2021
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Alocilja, E.C. 2021. Invited discussant for the Project Kickoff and Consultative meeting on National Campaign of Fecal sludge Management (FSM), sponsored by the Society of Public Health Engineers Nepal (SOPHEN) in association with the Environment and Public Health Organization (ENPHO), supported by Bill and Melinda Gates foundation, Virtual, Jan. 6, 2021. Invited speaker/discussant.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Flores, M.S., Fernando, L., Amalin, D. Dimamay, M., Bautista, M.A., Alocilja, E.C. 2021. Rapid Magnetic Nanoparticle (MNP)-based Detection of Dengue Virus (DENV) and Japanese Encephalitis Virus (JEV) in Culture Samples", 53rd PMCP Anniversary and Annual Scientific Conference, Manila, Philippines, Best Poster Paper award, July 6-7, 2021.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Purificacion, M., Dote, A., Juan, A.S., Babaan, M.D., Franco, A.J., Dela Cueva, F., Alvindia, A., Amalin, A., Alocilja, E., Fernando. L. 2021. Plant Disease Surveillance Using Carbohydrate-Functionalized Magnetic Nanoparticles, Federation of Plant Science Associations of the Philippines (FPSAP) Scientific Conference Second Place Poster Award, Crop Production and Management/Protection Category, Manila, Philippines, September 1-2, 2021.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Boodoo, C. and Alocilja, E.C. 2021. Organizers and Moderators of the 2021 Global Alliance on Research Diagnostics (GARD) Forum: Bridging Technologies and Market Needs, virtual, June 25-26, 2021.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Payumo JG, Alocilja E, Boodoo C, Luchini-Colbry K, Ruegg P, McLamore E, Vanegas D, Briceno RK, Castaneda-Sabogal A, Watanabe K, Gordoncillo MJ, Amalin D, Fernando L, Bhusal N. 2021. Next Generation of AMR Network. Encyclopedia. 2021; 1(3):871-892
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Sharief S, Chahal P, Alocilja EC. 2021. Application of DNA sequences in anti-counterfeiting: Current progress and challenges. International Journal of Pharmaceutics, Volume 602, 1 June 2021, 120580
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: McLamore ES, Alocilja EC, Gomes C, Gunasekaran S, Jenkins D, Li Y, Mao Y, Nugen S, Reyes De Corcuera J, Takhistov P, Tsyusko O, Cochran JP, Tzeng TR, Yoon JY, Yu C, Zhou A. 2021 (preprint in 2020). A FEAST of Biosensors: Food, Environment, Agriculture, Science, Technology (FEAST) and Engineering in North America. Biosensors and Bioelectronics, 2021 April 15;178:113011, 24 p.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Victoria Morgan, Lisseth Casso-Hartmann, David Bahamon-Pinzon, Kelli McCourt, Robert G. Hjort, Sahar Bahramzadeh, Irene Velez-Torres, Eric McLamore, Carmen Gomes, Evangelyn C. Alocilja, Nirajan Bhusal, Sunaina Shrestha, Nisha Pote, Ruben Kenny Briceno, Shoumen Palit Austin Datta, and Diana C. Vanegas3. 2020. Sensor-as-a-Service: Convergence of Sensor Analytic Point Solutions (SNAPS) and Pay-A-Penny-Per-Use (PAPPU) Paradigm as a Catalyst for Democratization of Healthcare in Underserved Communities. Diagnostics, 10(1):22 (22pg).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2020 Citation: Alocilja, E. 2020. Nano-enabled Biosensor for Rapid Screening, Detection, and Monitoring of Infectious Diseases, Department of Chemistry and Biochemistry, Kettering University, December 10, 2020.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2020 Citation: Alocilja, E. 2020. Research Opportunities for Students at MSU. Alumni Distinguished Scholarship Program, Kellogg Center, East Lansing, Michigan, January 31 and February 7, 2020.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2020 Citation: Alocilja, E. Nanoparticle-based biosensors for for rapid detection of antimicrobial resistance in the food supply chain., NC-1194 annual meeting, virtual, May 20, 2020
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2020 Citation: Caliskan-Aydogan, O., Alocilja, E.C., Morphological Changes of Salmonella Typhimurium Exposed to Imipenem, Poster presentation, ASM Microbe Online, June-August 2020.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Dester, E. and Alocilja, E.C. 2021. Innovation Challenge, 2021 Global Alliance on Research Diagnostics (GARD) Forum: Bridging Technologies and Market Needs, virtual, June 25-26, 2021.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: Dester, E., Sharief, S., Alocilja, E.C. 2021. Utilization of magnetic nanoparticles for extraction and concentration of bacteria in food matrices. American Society of Agricultural and Biological Engineers (ASABE), Virtual, July 12-16, 2021


Progress 10/01/19 to 09/30/20

Outputs
Target Audience:The various target audiences reached by our efforts during this reporting period were undergraduate students and graduate students at MSU, high school students, parents and family members of students, faculty and students from other universities, industry personnel, government personnel, extension personnel, and scientists. We have also reached many students, faculty members, and scientists from institutions of higher education in the Philippines, Japan, Peru, Mexico, Nepal, India, and Uganda. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained 14undergraduate students, 2 MS students, 3 PhD students, 2 PhD scholars, 1visiting scholar, and 1 high school student. These students and scientists will become the future research leaders in the emergingfield of biosensing for global health, biodefense, food safety, water quality, and product integrity. How have the results been disseminated to communities of interest?Research results have been disseminated through journal publications and conference presentations as well as through interactions with industry personnel. In addition to research dissemination, we continue to strengthen our global collaboration and networking in the Philippines, Japan, Thailand, Peru, Mexico, Nepal, India, and Uganda. What do you plan to do during the next reporting period to accomplish the goals?The next step of the project is to conduct (1) validation studies of the magnetic nanoparticles to determine the concentration factor in foodborne, waterborne, and human pathogens under complex matrices, (2) validation studies of the gold nanoparticles for colorimetric signal generation for genomic DNA detection, (3) design and development studies to automate signal read-out using smartphones, and (4) comparative studies between our proposed methods and gold standard protocols.

Impacts
What was accomplished under these goals? Glycan-coated magnetic nanoparticles (MNP) were synthesized and characterized in terms of size, surface charge, and their interaction with foodborne bacteria. The MNPs wereused to isolate and concentrate foodborne bacteria from simple and complex food matrices in large volumes (100 mL or 25 g). Results showed that the MNPs were able to concentrate Salmonella enterica serovar Enteritidis in spinach by a factor of 6. The concentration factor (CF) increased as cell concentration decreased. This means that the MNP is able to extract most at lower bacterial contamination in food. For example, CF greater than 10 was observed in bacterial concentration less than 104 colony forming units/milliliter (cfu/mL). These results are very encouraging as natural contamination tend to have low bacterial concentration and infective doses of foodborne illnesses tend to be low as well. Results also showed that S. Enteritidis and L. monocytogenes could be isolated and concentrated by MNP from beef sausages with Salmonella having a higher CF value (6.5) compared to L. monocytogenes (1.3). A similar study was conducted for raw chicken where the MNP concentrated Salmonella by a factor of 2. Results showed that the type of matrix affect CF. MNP had highest CF for Listeria in beef sausages compared to Listeria CF in broccoli, spinach, and raw chicken wash, which had the lowest CF among all samples (<2). Binding between the bacteria and MNP was visualized using a transmission elecron microscope (TEM) and a laser microscope (LM). It is interesting to note that in all images, the MNP was attached close to the tip of the bacerial cell. To validate this observation, 100 individual bacterial cells bound to MNP were analyzed for the position of binding. Results showed that MNP bound to specific regions of the cells. Our research work in the Philippines (funded by Philippine government agencies) focused on developing nanoparticle-based biosensors for the rapid surveillance and on-site detection of diseases of economically important crops. These pathogens include Ralstonia solanacearum,Phytophtora infestans, Fusarium oxysporum,MungbeanYellow Mosaic Virus,Cercospora arachidicola,Potato Virus Y, andBanana Bunchy Top Virus. Success of these projects will benefit not only the Phlippines but also the US andthe world in sustaining the food supply and securing food for all.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Briceno RK, Sergent SR, Benites SM, Alocilja EC. 2019. Nanoparticle-based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture. Diagnostics, 9:222 (14p).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: McLamore E, Datta SPA, Morgan V, Cavallaro N, Kiker G, Jenkins D, Rong Y, Gomes C, Claussen J, Vanegas D, Alocilja E. 2019. SNAPS: Sensor Analytics Point Solutions for Detection and Decision Support Systems. Sensors, 19:4935 (22p).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Yrad FM, Catanares JM, Alocilja EC. 2019. Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor. Diagnostics, 9:74-88.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Franco, AJ, Merca FE, Rodriguez MS, Balidion JF, Migo VP, Amalin DM, Alocilja EC, Fernando LM. 2019. DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods. Physiological and Molecular Plant Pathology. 107 (2019):14-20.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Anaximandro G�mez-Velasco, Jorge L. Le�n-Cort�s, Cristina Gordillo-Marroqu�n, H�ctor Javier S�nchez-P�rez, Evangelyn C. Alocilja, Sergio G. Mu�oz-Jim�nez, A. Bencomo-Alerm, Nat�n Enr�quez-R�os, Letisia Jonap�-G�mez, Adriana G�mez-Bustamante. 2019. Uso de nanopart�culas magn�ticas y un biosensor para el diagn�stico y monitoreo de enfermedades infecciosas emergentes, re-emergentes y tropicales desatendidas (English title: Use of magnetic nanoparticles and a biosensor for the diagnosis and monitoring of emergent, re-emergent and neglected tropical infectious diseases). Rev Enf Emerg 18(1):23-31.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: Alocilja EC and Dester E. 2019. Nano-Biosensor-Antibiogram for Rapid Screening of Antimicrobial Resistance. Chemistry and Biology of Pathogen Symposium, Michigan State University, May 16, 2019. Sponsored by Agilent.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Alocilja EC. 2019. Research Opportunities for Undergraduate Students at Michigan State University. Alumni Distinguished Scholarship Recruitment Program, Michigan State University, January and 2019. (Invited Speaker).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Alocilja EC. 2019. Anti-counterfeiting Technologies (Spark Table). Anti-Counterfeiting and Product Protection Centers Brand Protection Strategy Summit. Marriott, East Lansing, Michigan, September 24-25, 2019. (Invited Speaker).
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Bhusal N, Shrestha S, Pote N, and Alocilja E. 2019. Nanoparticle-based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods. Biosensors, 9(1):1-11.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: Fernando LM, Joram D, Mendoza R, Suministrado MKC, Lopez IAP, Atienza MTJA, Franco AF, Noe JR, Perez MTP, Trinidad LC, Aguilar EA, Amalin DA, Alocilja EC, Elegado FB. 2019. Nanobiosensors for the detection of pathogens in food and environmental samples. 1st Symposium of JSPS Core-to-Core Program Center of Excellence in Health Risk Assessment for Adaptation to Climate Change. Manila, Philippines, November 11-12, 2019.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: Alocilja EC and Bhusal N (presentor). 2019. USAID-Nepal Partnership: Next-Generation Antimicrobial Stewardship in Health-Food-Environment. Building Nepals Institutional Capacity Workshop, Kathmandu University, November 19, 2019. (Invited speaker, presented by Dr. Bhusal)
  • Type: Conference Papers and Presentations Status: Other Year Published: 2019 Citation: Alocilja EC. 2019. Smartphone-enabled Nano-Biosensing for Rapid Screening of Antimicrobial Resistance, Tuskegee University, Tuskegee, Alabama, Spet. 3, 2019. (Invited speaker)
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Alocilja EC and Dester E. 2019. Nano-Biosensor-Antibiogram for Rapid Screening of Antimicrobial Resistance. Symposium on the Chemistry and Biology of Pathogens. Michigan State University, May 16, 2019. (Invited Speaker).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Alocilja EC. 2019. Global Alliance for Rapid Diagnostics: Leveraging Global Talents To Save Lives. Latin- American Research and Education Consortium, Michigan State University, August 28, 2019. Union Bldg., Sept. 12-13, 2019. (Invited Speaker).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Alocilja EC. 2019. Multiplex Nano-Biosensor for B. anthracis DNA Detection. MSU-US Senate Homeland Security Meeting. Administration Bldg., Michigan State University, August 28, 2019. (Invited Speaker).


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:The various target audiences reached by our efforts during this reporting period were undergraduate students and graduate students at MSU, high school students, parents and family members of students, faculty and students from other universities, industry personnel, government personnel, extension personnel, and scientists. We have also reached many students, faculty members, and scientists from institutions of higher education in the Philippines, Japan, Peru, Mexico, Nepal, India, and the Dominican Republic. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained 10 undergraduate students, 2 PhD students, 1 MS student, and 1 high school teacher. The students won several awards. We have also trained one scientist from the Philippines, one scientist from Nepal, and two scientists from India on the use of our technologies. These students and scientists will become the future research leaders in the emerging field of biosensing for global health, biodefense, food safety, water quality, and product integrity. How have the results been disseminated to communities of interest?The research results have been disseminated through journal publications and conference presentations as well as through interactions with industry personnel. In addition to research dissemination, I continue to strengthen our global collaboration and networking. What do you plan to do during the next reporting period to accomplish the goals?The next step of the project is to conduct (1) validation studies of the magnetic nanoparticles to determine the concentration factor in foodborne, waterborne, and human pathogens under complex matrices, (2) validation studies of the gold nanoparticles for colorimetric signal generation for genomic DNA detection, (3) design and development studies to automate signal read-out using smartphones, and (4) comparative studies between our proposed methods and gold standard protocols.

Impacts
What was accomplished under these goals? WHO declared that health is a fundamental human right - - the right of every human being without distinction of race, religion, political belief, economic or social status. However, malnutrition and tuberculosis (TB) interact with each other and they are both problems of considerable magnitude in most of the developing countries. TB mortality rates in different economic groups tend to vary inversely with their economic levels. Infections like TB can reduce appetite, decrease the body's absorption of nutrients, and alter metabolism leading to wasting. Globally, about 10 million people develop tuberculosis (TB) and it is the cause of an estimated 1.3 million deaths among HIV-negative people and an additional 300,000 deaths among HIV-positive people. Furthermore, multidrug-resistant and extensively drug-resistant TB (MDR/XDR-TB) have become global public health threats. Current methods of TB detection are either time consuming, not sensitive, complex to operate, or expensive. Specifically, culture method is the "gold standard" but it takes 8 weeks to get results, acid-fast bacilli sputum smear microscopy is inexpensive and fast but has low sensitivity (38%-67%), serological tests are not reliable, and X-ray is expensive and requires a lab facility. Several molecular techniques have been commercialized for detecting Mycobacterium tuberculosis and mutations in rpoB and katG genes that are more sensitive, however, they are not necessarily accessible or affordable to those who need them the most. We believe in WHO's declaration. No one should get sick and die just because they are poor. Thus, our strategy is to develop rapid, highly sensitive, and affordable biosensing systems in order to detect TB and drug resistant TB early so that patients can get treatment early, while the immune system is still high and before they experience malaise. It has been shown that nutritional status of TB patients improves during TB chemotherapy. In 2018, we finally published our first peer-reviewed journal paper on our TB detection method called the Nanoparticle-based Colorimetric Biosensing Assay (NCBA). NCBA was compared with SSM for the detection of pulmonary tuberculosis (PTB) in sputum samples collected from PTB patients prior to receiving treatment. Results showed that all TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM and increased the grading of samples from "1+" to "2+". These results show that paucibacillary cases (e.g., children, TB/HIV, drug-resistant TB, and patients with TB comorbidities) could now be easily detected using the NCBA technique where SSM would have missed them (false negative). NCBA uses only a simple magnet and does not require any major equipment, electricity or refrigeration, can be completed in less than 20 min and only costs $0.10 per test. Thus, this system can be easily incorporated in clinical practice to enhance TB diagnosis, especially in low resource communities. In 2018, Our research on nanoparticle-based biosensors was also featured in a podcast by the National Nanotechnology Initiative of the US government. It is now a permanent resource online available to all those interested on the topic. Furthermore, our research was featured by the MSU Honors College brochure, which are distributed to new faculty and students at Michigan State University.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja, E. Nanoparticle-based Technologies for Global Health and Food Sustainability. NC-1194 Annual Meeting, Mount Holyoke College, Massachusetts, June 4-5, 2018.
  • Type: Journal Articles Status: Accepted Year Published: 2018 Citation: Alocilja E and Dester E. Antibiogram-Biosensor for Rapid Screening of Antimicrobial Resistance. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 30, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Thekkudan V. and Alocilja E. Antimicrobial activity of N-halamine-based chitosan-PVA composite films against methicillin resistant Staphylococcus aureus. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 30, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja E. and Shan S. Synthesis of antimicrobial N-halamine-based chitosan-PVA biodegradable films. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 30, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja E. and Bassett C. Effect of Antimicrobial Film on the Shelf Life of Various Leafy Vegetables. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 30, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja E. and Chandra A. Purification of Untreated Wastewater Using Magnetic Nanoparticles. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 31, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja E. and Fox A. One-Stop-Shop For Anti-Counterfeiting Technologies. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 31, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Yrad F. and Alocilja E. Gold Nanoparticle Synthesis and Development of Lateral Flow Biosensor for Dengue Detection. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 31, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Jones R., Cannon T., and Alocilja E. DNA Lock n' Key Anti-Counterfeiting Technology. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 31, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Dester E. and Alocilja E. Visual Test for Rapid Screening of Infectious Diseases and Their Susceptibility to Antibiotics. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 31, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Dester, E. and Alocilja, E. Visual Test for Rapid Detection of Diseases and their Antibiotic Susceptibility. Mid-Michigan Symposium for Undergraduate Research Experiences (Mid-SURE), Huntington Club, July 24, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Thekkudan, V., Shan S., and Alocilja, E. Antimicrobial activity of treated chitosan-PVA films in drug-susceptible and drug resistant bacteria. Graduate Engineering Symposium, Michigan State University, East Lansing, Michigan, March 29, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Thekkudan, V. Alocilja, E. Functionalized Magnetic Nanoparticles to Capture Drug-Resistant and Drug-Susceptible Staphylococcus aureus and Salmonella Typhimurium. 2018 Graduate Academic Conference, MSU Union, Michigan State University, East Lansing, MI, Feb. 17, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Thekkudan, V., Shan, S., and Alocilja, E. Antimicrobial activity of treated chitosan-PVA film in eliminating drug resistant bacterial contamination in meat. ESPP Conference, Kellogg Center, Michigan State University, East Lansing, MI, November 1, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Jones R, Cannon T, Alocilja EC. 2018. DNA Lock n Key Anti-Counterfeiting Method. University Undergraduate Research and Arts Forum, Michigan State University, April 13, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Bassett C, Shan S, Alocilja EC. 2018. Effect of Antimicrobial Film on the Shelf Life of Leafy Vegetables. University Undergraduate Research and Arts Forum, Michigan State University, April 13, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Bugenske A, Dester E, Vasher M, and Alocilja EC. 2018. Rapid User-Friendly Diagnostic Test for Detection of Pathogens. University Undergraduate Research and Arts Forum, Michigan State University, April 13, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Fox A, Weaver K, and Alocilja EC. 2018. One-Stop Shop for Anti-Counterfeiting Technologies. University Undergraduate Research and Arts Forum, Michigan State University, April 13, 2018
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Briceno RK, Alocilja EC, et al. 2018. Novel Method for Rapid Diagnosis of TB Using Magnetic Nanoparticles in La Libertad Region Peru. International Conference on Nanotechnology for the Diagnosis of Tuberculosis, Universidad Mariana, Pasto, Colombia, March 60-8, 2018. Invited Speaker, all expenses (airline ticket, lodging, meals, etc.) paid for by Mariana University.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja, E. 2018. Nano-biosensor technologies for the Philippine setting. Invited Speaker, all expenses (airline ticket,lodging, meals, etc.) paid for by De La Salle University, Aug. 23, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja, E. 2018. Nano-enabled biosensor for plant disease detection. Alocilja matting and antibiogram-biosensor for rapid screening of antimicrobial resistance. Invited Speaker, all expenses (airline ticket, lodging, meals, etc.) paid for by De La Salle University, Aug. 24, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja, E. 2018. Nano-Biosensors Research Program in the Philippines, PCAARRD Bldg., Los Banos, Philippines, Invited Speaker, all expenses (airline ticket, lodging, meals, etc.) paid for by De La Salle University, Aug. 29, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja, E. 2018. Nano-biosensors for rapid screening, diagnosing, and monitoring of antimicrobial resistance and dengue. Invited Speaker, all expenses (airline ticket, lodging, meals, etc.) paid for by Ehime University, Sept. 3, 2018
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja EC. 2018. Research Opportunities for Undergraduate Students at Michigan State University. Alumni Distinguished Scholarship Recruitment Program, Michigan State University, February 2018. Invited Speaker.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Gordillo CM, Gomez AV, Sanchez HP, Pryg K, Shinners J, Murray N, Munoz-SG, Bencomo AA, Gomez, AB, Janapa LG, Enriquez NR, Martin M, Romero NS, and Alocilja EC. 2018. Magnetic Nanoparticle-based Biosensing Asasy Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis. Biosensors, 8(4):128-141.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Alocilja EC, Sharief SA, Kriti N, and Chahal P. 2018. Combining Blockchain, DNA, and Nanotechnology for Product Authentication and Anti-Counterfeiting. Brand Protection Professional, (Dec. 20, 2018).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Matta LL and Alocilja EC. 2018. Carbohydrate Ligands on Magnetic Nanoparticles for Centrifuge-free Extraction of Pathogenic Contaminants in Pasteurized Milk. J Food Protection, 81(12):1941-1949. (Dec. 2018)
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Zeeshan N, Daya KS, Tirumalai PS, and Alocilja E. 2018. Impedance and Magnetohydrodynamic Measurements for Label Free Detection and Differentiation of E. coli and S. aureus using Magnetic Nanoparticles. IEEE Transactions on NanoBioscience, 17(4):443-448. (Oct. 2018).
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Matta LL, Karuppuswami S, Chahal P, and Alocilja EC. 2018. AuNP-RF Sensor: An innovative application of RF technology for sensing pathogens electrically in liquids (SPEL) within the food supply chain. Biosensors and Bioelectronics, 111:152-158.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Matta LL, Harrison J, Deol G, and Alocilja EC, 2018. Carbohydrate-functionalized Nano-Biosensor for Rapid Extraction of Pathogenic Bacteria Directly from Complex Liquids with Quick Detection Using Cyclic Voltammetry. IEEE Transactions on Nanotechnology, 17(5):1006-1013.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Matta LL and Alocilja EC. 2018. Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling. Biosensors and Bioelectronics, 117:781-793
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Karuppuswami S, Matta LL1, Alocilja EC, and Chahal P3. 2018. A wireless RFID compatible sensor tag using gold nanoparticle markers for pathogen detection in the liquid food supply chain. IEEE Sensors Letters, 2(2), 4pg
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Baetsen-Young AM, Vasher M1, Matta L, Colgan P, Alocilja E, Day B. 2018. Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles. Biosensors and Bioelectronics, 101:29-36.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja E. and Mitra A. Magnetic Nanoparticles for the Removal of UV-Resistant Bacteria in Influent Wastewater. Oral presentation at the 2018 Annual ASABE International Meeting, Detroit, USA, July 30, 2018.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Alocilja, et al., 2018. Nanotechnology-enabled Biosensor for Rapid Detection of Tuberculosis. International Conference on Nanotechnology for the Diagnosis of Tuberculosis, Universidad Mariana, Pasto, Colombia, March 6-8, 2018. Invited Speaker, all expenses (airline ticket, lodging, meals, etc.) paid for by Mariana University.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:The various target audiences reached by our efforts during this reporting period were undergraduate students and graduate students at MSU, high school students, parents and family members of students, faculty and students from other universities, industry personnel, government personnel, extension personnel, and scientists. We have also reached many students, faculty members, and scientists from institutions of higher education in the Philippines, Peru, Colombia, Mexico, and Poland. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained 16 undergraduate students, 3 PhD students, 2 high school students, and 1 high school teacher. The students won several awards. We have also trained one scientist from the Philippines, two scientists from Peru, one scientist from Nepal, and two scientists from India on the use of our technologies. These students and scientists will become the future research leaders in the emerging field of biosensing for global health, biodefense, food safety, water quality, and product integrity. How have the results been disseminated to communities of interest?The research results have been disseminated through journal publications and conference presentations as well as through interactions with industry personnel. In addition to research dissemination, I continue to strengthen our global collaboration and networking. What do you plan to do during the next reporting period to accomplish the goals?The next step of the project is to continue the synthesis of various bio-functionalized and biomimetic nanoparticles, develop handheld biosensor devices, and validate the biosensors in various food matrices, clinical samples, and environmental samples. We will pursue industry collaborations to prepare toward eventually commercializing the biosensor devices and nanoparticles.

Impacts
What was accomplished under these goals? Our major achievements can be summarized as follows: Our research was Finalist for the 2017 Manufacturing Innovation Leadership Award (US national award) One of students received the 2017 State of Michigan Stockholm Junior Water Prize Award; student represented the State of Michigan to the US Stockholm Junior Water Prize Competition Technology transfer was our continuing activity. We received two US patents and filed several new invention disclosures while improving the technologies that are being reviewed by the US Patent and Trademark Office. Our technologies continue to be validated in clinical and biological samples (human, animal, and plant) for rapid disease and microbial-contaminant detection in our lab at MSU as well as with our collaborators around the world, such as Nepal, India, Peru, Colombia, Philippines, and Poland. We are developing new antimicrobial nanoparticles and antimicrobial films to help reduce foodborne illness. Our technology on anti-counterfeiting devices is continually featured in the Science of Innovation educational program by the National Science Foundation, US Patent and Trademark Office, and NBC Learn as a national resource to encourage and recruit K-12 students to the science fields. The video is entitled "Science of Innovation: Anti-Counterfeiting Devices" and can be viewed at w.nbclearn.com/innovation/cuecard/62970. This material will impact thousands of K-12 students and teachers not only in the US but also around the world. My TEDMED talk continues to gain audiences from many sectors. The TED talk is featured in the following website: http://www.youtube.com/watch?v=QGauiO0Eev0. Our presentations at professional meetings allowed the dissemination of our research work to a broader group of researchers and potential users both in the US and around the world. We have signed Material Transfer Agreements with many institutions around the world to validate our technologies.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Luo Y and Alocilja EC. 2017. Portable nuclear magnetic resonance biosensor and assay for a highly sensitive and rapid detection of foodborne bacteria in complex matrices. J Biological Engineering, 11:14, 8 p.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Contreras J, Fernando L, Alocilja E, Salazar F, Bacay B. 2016. Fabrication of a nanoparticle-based sensor for the detection of dengue virus-3 in Aedes aegypti. International Journal of Sciences: Basic and Applied Research, 26(3): 138-157.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Lim D, Villame RG, Qui�ones GJ, de Vera D, Notorio R, Fernando L, Alocilja E. 2017. Alocilja Magnetic Nanoparticles capture Escherichia coli O157:H7 isolates. Philippine Journal of Pathology Open Access, 2(2): 47-49.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja et al. 2017. Nanotechnology-based biosensors for rapid detection of infectious and antimicrobial resistant pathogens. Case: Tuberculosis. VIII Congreso International de Investigacion Ciencia, Tecnolog�a e Innovaci�n para el Desarrollo, Chimbote, Peru, November 16-18, 2017. Invited Speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja et al. 2017. Exploiting Bio-magnetic properties for a label-free extraction and concentration of bacterial pathogens from complex matrices. Annual Meeting of the American Chemical Society, Washington, D.C. August 24, 2017. Invited Speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja E. 2017. Global validation of the nano-enabled biosensor for rapid diagnosis and surveillance of infectious diseases. 2017 TechConnect World Innovation Conference and Expo, Washington, D.C., May 15-17, 2017. Invited Speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja et al. 2017. New developments in rapid diagnostic technology for antimicrobial resistance. 12th OIE Seminar and 18th World Association of Veterinary Laboratory Diagnosticians Symposium, Sorrento, Italy, 7-10 June 2017. Invited Speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja EC. 2017. Nanotechnology for rapid diagnosis of infectious diseases. Research Institute for Tropical Medicine, Manila, Philippines, August 29, 2017. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja EC. 2017. Nano-biosensors for rapid diagnosis of human infectious diseases. Presentation was used as discussion for potential collaboration with Dr. Ram, Dr. Bhusal, and others at Kathmandu University, Nepal. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja EC. 2017. Nano-biosensors for rapid diagnosis of animal diseases. Presentation was used as discussion for potential collaboration with Dr. Bhattarai and others at the Agriculture and Forestry University, Nepal. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja et al. 2017. Development of biosensors and antimicrobial agents for rapid detection and control of antimicrobial resistant infectious pathogens. 2017 Annual Meeting of the Institute of Food Technologists, Las Vegas, Nevada, June 25-28, 2017. Invited Speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja E. 2017. Product DNA Project. Supply Chain Management Council Meeting, Henry Center, Michigan State University, January 18, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja E. 2017. Nano-biosensor research at MSU. Alumni Distinguished Scholarship Competition. Kellogg Center, Michigan State University, January 27, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja E. 2017. DNA-AuNP technology for anti-counterfeiting. Anti-Counterfeiting and Product Protection Annual Meeting, Lansing Convention Center, October 3-5, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Deol G, Chandra A, Matta L, Alocilja E. 2017. Label-free Microbial Detection and Identification Using a Handheld Potentiostat. University Undergraduate Research and Arts Forum, Michigan State University, April 7, 2017. First Place Poster Award
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Alocilja EC. 2017. Nano-biosensors for rapid diagnosis of medical and agricultural diseases. De La Salle University, Manila, Philippines, August 30, 2017. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Jones R, Cannon T, Vasher M, Baetsen-Young A, Day B, and Alocilja E. 2017. DNA Detection Using PCR-less Methods. University Undergraduate Research and Arts Forum, Michigan State University, April 7, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Vasher M, Bugenske A, Jyothi L, Briceno R, and Alocilja E. 2017. Rapid, User-Friendly Diagnostic Test Using Functionalized Magnetic Nanoparticles. University Undergraduate Research and Arts Forum, Michigan State University, April 7, 2017
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Chandra A, Shan S, Alocilja E. 2017. Extraction of Bacteria from Untreated Wastewater. University Undergraduate Research and Arts Forum, Michigan State University, April 7, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Bassett C, Shan S, Alocilja E. 2017. Reducing Food Waste through Novel Antimicrobial Films. University Undergraduate Research and Arts Forum, Michigan State University, April 7, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Chinnusamy A, Shan S, Alocilja E. 2017. Antimicrobial Films to Combat Listeriosis. University Undergraduate Research and Arts Forum, Michigan State University, April 7, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Mishra A and Alocilja E. 2017. Utilizing Magnetic Nanoparticles to Remove UV Resistant Salmonella in Influent Wastewater. RET Program, Michigan State University, August 4, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Burton K, Mishra A, Matta L, and Alocilja E. Different Methods of Removing bacteria from wastewater. RET Program, Michigan State University, August 4, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Cannon T, Jones T, Bhusal N, Bhattarai R, and Alocilja E. 2017. PCR-less DNA Detection Using Functionalized Gold Nanoparticles. Mid-SURE, Huntington Club Spartan Stadium, Michigan State University, July 26, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Rodriguez K and Alocilja E. 2017. Evaluation of Re-using Magnetic Nanoparticles in Removing Bacteria from River Water. Mid-SURE, Huntington Club Spartan Stadium, Michigan State University, July 26, 2017.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Baasher F and Alocilja E. 2017. Effect of Antimicrobial Nanoparticles on Size and Growth Rate of Bacteria. Mid-SURE, Huntington Club Spartan Stadium, Michigan State University, July 26, 2017.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:The various target audiences reached by our efforts during this reporting period were undergraduate students and graduate students at MSU, high school students, parents and family members of students, faculty and students from other universities, industry personnel, government personnel, extension personnel, and scientists. We have also reached many students, faculty members, and scientists from institutions of higher education in Nepal, India, Sri Lanka, Philippines, Indonesia, Thailand, Peru, Colombia, Mexico, South Africa, and Poland. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained 13 undergraduate students, 2 PhD students, 1 high school student, and 1 high school teacher. The students won three awards. We have also trained two scientists from the Philippines, two scientists from Peru, two scientists from Nepal, and one medical professional from India on the use of our technologies. These students and scientists will become the future research leaders in the emerging field of biosensing for global health, biodefense, food safety, water quality, and product integrity. How have the results been disseminated to communities of interest?The research results have been disseminated through journal publications and conference presentations as well as through interactions with industry personnel. In addition to research dissemination, I continue to strengthen our global collaboration and networking. What do you plan to do during the next reporting period to accomplish the goals?The next step of the project is to continue the synthesis of various bio-functionalized and biomimetic nanoparticles, develop handheld biosensor devices, and validate the biosensors in various food matrices, clinical samples, and environmental samples. We will pursue industry collaborations to prepare toward eventually commercializing the biosensor devices and nanoparticles.

Impacts
What was accomplished under these goals? Our major achievements can be summarized as follows: Our technologies continue to be validated in clinical and biological samples (human, animal, and plant) for rapid disease and microbial-contaminant detection in our lab at MSU as well as with our collaborators around the world. We are developing new antimicrobial nanoparticles and antimicrobial films to help reduce foodborne illness. Our technology on anti-counterfeiting devices is continually featured in the Science of Innovation educational program by the National Science Foundation, US Patent and Trademark Office, and NBC Learn as a national resource to encourage and recruit K-12 students to the science fields. The video is entitled "Science of Innovation: Anti-Counterfeiting Devices" and can be viewed at www.nbclearn.com/innovation/cuecard/62970. This material will impact thousands of K-12 students and teachers not only in the US but also around the world. My TEDMED talk continues to gain audiences from many sectors. The TED talk is featured in the following website: http://www.youtube.com/watch?v=QGauiO0Eev0. Our presentations at professional meetings allowed the dissemination of our research work to a broader group of researchers and potential users both in the US and around the world. Technology transfer was our continuing activity. We received two US patents and filed several new invention disclosures while improving the technologies that are being reviewed by the US Patent and Trademark Office. We have signed Confidential Disclosure Agreements (CDA) with two private companies to explore commercialization of our technologies.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC, Pryg K, Shinners J, Murray N. 2016. Nano-assisted TB smear microscopy and detection. Medical Conference, El Colegio de la Frontera Sur, San Cristobal de las Casas, Chiapas, Mexico. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC, Matta L, Quilantang M, Taylor N, Almeida O, Pinheiro B, Pryg K, Shinners J, and Murray N. 2016. Development and validation of a nano-enabled self-reporting biosensor (SRB) for rapid screening of microbial agents in the food supply chain. IFT16, Chicago, Illinois, July 16-19, 2016. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC, 2016. Development of a self-reporting biosensor (SRB) for rapid screening of microbial pathogens. Invited seminar, University of Missouri, Columbia, Missouri, August 1, 2016. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC. 2016. Novel Antimicrobial Technologies Against Foodborne Pathogens. Invited seminar, Dow Chemicals, Freeport, Texas, August 4, 2016. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC. 2016. Nanotechnology for Prevention, Detection, and Control of Infectious Diseases: A Platform for Global Multidisciplinary Public-Private Collaborative Research. VII Congreso Internacional De Investigacion Ceintifica 2016, November 3-5, 2016. Invited speaker.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Matta L and Alocilja EC. 2016. Functional Ligands as Antibody-mimics for the Expedient Extraction of Salmonella Enteritidis, E. coli O157:H7 and Bacillus cereus in Fresh Milk. International Association for Food Protection Annual Meeting, St. Louis, MO, July 30-August 3, 2016
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC, Matta L, Harrison J, Taylor N, and Deol G. 2016. Novel Antimicrobial Technologies Against Foodborne Pathogens. International Association for Food Protection Annual Meeting, St. Louis, MO, July 30-August 3, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC, Matta L, Quilantang M, Taylor N, Almeida O, Pinheiro B, Pryg K, Shinners J, and Murray N. 2016. Multifunctional Biomimetic Receptors for Cell Binding and Concentration. Institute of Biological Engineering Annual Meeting, Greenville, South Carolina, April 7-9, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Alocilja EC, Matta L, Taylor N, Deol G, Harrison J. 2016. Synthesis and Verification of Multifunctional Antimicrobial Glyco-Amino Acid Compounds Against Bacterial Pathogens. Multifunctional Biomimetic Receptors for Cell Binding and Concentration. Institute of Biological Engineering Annual Meeting, Greenville, South Carolina, April 7-9, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Baetsen-Young A, Vasher M, Alocilja E, and Day B. 2016. DNA-nanosensor based diagnostics of plant pathogens. 2016 American Phytopathological Society Annual Meeting, Tampa, Florida, July 30-August 3, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Harrison J, Matta L, and Alocilja EC. 2016. Antimicrobial coating for food packaging. University Undergraduate Research and Arts Forum, Michigan State University, April 8, 2016. First Place Poster.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Lay L and Alocilja EC. 2016. Can Magnetic Nanoparticles Curb Tuberculosis Growth? University Undergraduate Research and Arts Forum, Michigan State University, April 8, 2016. First Place Poster.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Deol G, Matta, L, and Alocilja, E.C. 2016. Bacterial Growth Inhibition Utilizing Magnetic Nanoparticles. University Undergraduate Research and Arts Forum, Michigan State University, April 8, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Cannon T, Jones R, Quilantang M, Pryg K, and Alocilja EC. 2016. Rapid Detection of Foodborne Pathogens Using Biosensors. University Undergraduate Research and Arts Forum, Michigan State University, April 8, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Vasher M, Fernando L, and Alocilja EC. 2016. Dengue Virus Detection Using a Biosensor. University Undergraduate Research and Arts Forum, Michigan State University, April 8, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Pryg K, Murry N, Shinners J, and Alocilja EC. 2016. Development of Novel Diagnostics for Rapid Detection of Tuberculosis in Rural Areas. University Undergraduate Research and Arts Forum, Michigan State University, April 8, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Harrison J and Alocilja EC. 2016. Novel Antimicrobial Bioelectric Effect on Escherichia coli. Mid-Michigan Symposium for Undergraduate Research Experiences (Mid-SURE), Michigan State University, July 27, 2016.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Farrell J, Chandra A, and Alocilja E. 2016. Evaluating the Effectiveness of Household Produce Cleaning Methods on Bacteria. National Science Foundation Research Experience for Teachers (RET), Michigan State University, July 29, 2016.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Wang Y, Mazurek G, and Alocilja EC. 2016. Measurement of Interferon gamma Concentration Using an Electrochemical Biosensor. Journal of the Electrochemical Society, 163(5), B1-B6.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Yuan M1, Alocilja EC, and Chakrabartty S3. 2016. Self-powered wireless Affinity-based biosensor based on integration of paper-based microfluidics with self-assembling RFID Antennas. IEEE Transactions on Biomedical Circuits and Systems, 10(4), 799-806.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Alocilja EC, Jain P and Pryg K. 2016. Immunosensor for Rapid Extraction/Detection of Enteric Pathogens. Technology, 4(3), 194-200 (September 2016).
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Elegado FB, Ramirez TJ, Kawasaki S, Alocilja EC and Yeasmin S. 2016. Methods and Technology for Rapid and Accurate Detection of Foodborne Pathogens. In Md. Latiful Bari and Dike O. Ukuku (ed), Foodborne Pathogens and Food Safety, CRC Press, 133-161.