Source: CORNELL UNIVERSITY submitted to NRP
BENEFICIAL REUSE OF RESIDUALS AND RECLAIMED WATER: IMPACT ON SOIL ECOSYSTEM AND HUMAN HEALTH (FORMERLY W2170)
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1010641
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
W-3170
Project Start Date
Oct 1, 2016
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Crop & Soil Sciences
Non Technical Summary
Contact with urushiol (poison ivy/oak toxin) causes allergic response in 85% of the population. Plants are harvested and information on safe disposal is not available. These poisonous plants are conveyed to compost facilities.Compost research will determine the fate of urushiol during composting in large and small-scale settings; data will be analyzed in NY, PA and CA. Educational information will be developed and disseminated in English and Spanish.Poisonous plants are often added to compost piles as a feedstock but we don't know what the potency of the plant is as the plant decomposes and becomes part of the soil medium. Quality of compost is important for plant use and the people that apply it, or are in direct contact with it. Personal contact with poison ivy, Toxicodendron radicans, and poison oak, Toxicodendron rydbergii, at any time of the year can cause an allergic response in 85% of the U.S. population. Very little information is available on how to safely dispose of the plants, therefore by default it goes into home and municipal compost systems. The allergic reaction is caused by a family of organic compounds called urushiol. Urushiol is present in the sap, roots, stems, leaves and fruit of the plant and is released when the plant is damaged through cutting, mowing or brushing against it. Urushiol has been shown to remain on dead plants, clothing and contaminated objects for years. About 2 million cases of skin poisoning from poisonous plants are reported in the United States each year. Therefore, the question arises that if yard trimmings that contain poisonous plants are disposed of in compost piles, will urushiol degrade or still be active in the resulting compost? Cornell Waste Management Institute (CWMI) and others routinely get questions pertaining to this subject. Research on the decomposition of urushiol during the composting process will help to answer questions about how to dispose of the plant and how it would affect agriculture workers, gardeners and others that are in contact with compost. With this information, recommendations can be made on how to manage the disposal of poison ivy and poison oak without affecting human health and also how to manage the composting process when these plants are present.There are 3 states that will collaborate in this research New York, Pennsylvania and California. Trials and research will be conducted in each and the data collected will be combined into one database analyzed cooperatively. The New York and Pennsylvania teams will work with poison ivy. The California group will work with conduct the lab analyses.Are there jointly planned and interwoven activities between applied Research and Extension?The research will be completed and analyses conducted for bench scale reactors, small low turned piles and larger windrows with different feedstock mixed with the plants containing urushiol. When answers are secured, a fact sheet will be compiled so that people have this information and can protect themselves accordingly. The urushiol also has an effect on those harvesting the plant (horticulture industry) and processing the plant with different disposal methods. The research will give us information to advise all who may be exposed to it.Non-Cornell Collaborators: Dr. Robert Rynk, Associate Professor, SUNY Cobleskill. Performing benchscale compost trials.Dr. Fatih Buyuksonmez, Associate Professor, San Diego State University. Detection analysis for urushiol.Eric Carr, Rodale Institute, Composting poison ivy with different feedstocks.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1020110107040%
4032300114020%
7232420114040%
Goals / Objectives
Evaluate the short- and long-term chemistry and bioavailability of nutrients, potentially toxic inorganic trace elements, and pharmaceuticals and personal care products (TOrCs) in residuals, reclaimed water, and amended soils in order to assess the environmental and health risk-based effects of their application at a watershed scale. Specific tasks: (i) To develop and evaluate in vitro (including chemical speciation) and novel in vivo methods to correlate human and ecological health responses with risk-based bioavailability of trace elements and TOrCs in residuals and residual-treated soils. (ii) Predict the long-term bioavailability and toxicity of trace elements and TOrCs in residual-amended urban, agricultural and contaminated soils. (iii) Evaluate long-term effects of residuals application and reclaimed wastewater irrigation on fate and transport of nutrients, trace elements, TOrCs, and emergence/spread of antibiotic resistance in high application rate systems. (iv) Evaluate plant uptake and ecological effects of potentially toxic trace elements and TOrCs from soils amended with residuals and reclaimed wastewater. Evaluate the uses and associated agronomic and environmental benefits for residuals in agricultural and urban systems. Specific tasks: (i) Evaluate the ability of in situ treatment of contaminated soil with residuals to reduce chemical contaminant bioavailability and toxicity. (ii) Determine the climate change impacts of organic residuals end use options (i.e., C sequestration, N2O emissions). (iii) Quantify sustainability impacts such as water quality (reduced N impairment) and quantity benefits (increased plant available water, increased drought tolerance) and soil quality improvements associated with a range of organic residuals end uses. (iv) Explore the potential for waste by-products to be used in urban areas including urban agriculture, stormwater infrastructure, green roofs, and in urban green space. (v)Evaluate ecosystem services of degraded urban soils amended with residuals. (vi) Use tools such as life cycle assessment to understand and compare the impacts of a range of residuals end use/disposal options.
Project Methods
Three laboratory reactors will be used concurrently to reduce the experimental time frame. The primary experimental variable during the laboratory phase will be temperature (low - 35-40°C, moderate 40-60°C and high - 60-70°C). Otherwise, commonly recommended values will be established for other composting parameters (e.g. moisture, nutrient content). A designed feedstock mix will be used, including measured proportions of poison ivy. Forced aeration will be used to control airflow and temp. Reactor contents will be sampled at day 21, 28, 42, 56. Raman spectroscopy offers an effective way to detect quality and presence of other oils (Vaskova, H., Buckova, M., 2014) and fatty acid composition was also studied using GC-MS analysis. (Pereria, M, et al., 2006).Set up 3 compost piles with poisonous plants as part of the feedstock. Poisonous plants will be mixed with carbon feedstock. Plants will also be seeded into the piles in containers with holes so that compost microbes can access the plants so they can be removed and tested periodically. Samples of plant tissue, mixture and composted material will be taken and analyzed on day 0, 15, 30, 60, 90, 120, 150, 180 to determine if and how urushiol is reduced to other compounds or consumed by microbes through the compost process. Results will be analyzed, recommendations made and publicized through articles, a fact sheet and presentations. Recommendations based on science can then be made for both homeowners and municipalities. Results from this will be publicized and put into fact sheets and posted on Cornell eCommons and CWMI's website.

Progress 10/01/16 to 09/30/18

Outputs
Target Audience:The target audience of this research is the general public, as over 85% of the population experiences an allergic reaction to urushiol (the compound in poison ivy and poison oak that causes the skin rash). It is especially important to those that manage poison ivy or poison oak such as compost facility managers and those that use the product, such as landscapers, horticulturalists and home gardeners. Changes/Problems:The biggest problem we encountered was that the laboratory analysis could not be completed. Samples were shipped on ice to the lab of Fatih Buyuksonmez at San Diego State University for analysis of urushiol concentration. The lab was able to detect very little and was not able to quantification the urushiol. At that point the samples had been sitting for too long to give us reliable data if we could find a lab with more experience. What opportunities for training and professional development has the project provided? This project has realized the need for better understanding of laboratory analysis and sample preparation. How have the results been disseminated to communities of interest? Results (or lack of) will be presented at the BioCycle Conference in Portland, OR in April 2019. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Poisonous plants are often added to compost piles as a feedstock but we do not have information about their residual potency as the plant decomposes and becomes part of the soil medium. These toxins might affect people working on composting and people using compost. What happens to urushiol (the compound that causes the allergic reaction) during the composting process will help to answer questions on plant disposal and its effect on those in contact with compost. The research objectives of this project were to (1) assess the fate of the compound urushiol during the composting process and (2) develop research based recommendations for the disposal of poison ivy and oak and the resulting compost. Extensive literature searches were completed on the chemical structure and the dermatitogenic activity of urushiol. Based on the findings from this review, a field trial and a bench scale trial were set up. This final report includes what was done over the project period and what conclusions were drawn. Field Trial: Three compost piles were built in June of 2017 at the Education Center and 4-H Park in Otisville, NY. Poison ivy was harvested from the grounds and two piles were made by mixing partially composted leaf and yard waste at a ratio of 3:1 with the harvested plants. A third pile was created that was simply poison ivy (this was fenced off with a sign on it to discourage people from getting into it). Data loggers were placed in each of the piles to monitor temperature of the composting process at 6 hour intervals. Triplicate samples of each of the piles were taken on days 0, 2, 3, 9, 16, 23, 56, 86 and 120. All samples were put on ice and shipped to the lab of Fatih Buyuksonmez in San Diego, CA for analysis of urushiol concentration. Bench Trial: Poison ivy was harvested on June 21, 2017 along with weeds, small twigs and deciduous leaves. The material was put into the bench scale reactors for composting. Samples for days 0, 1, 2, 3 and 4 and 18 were pre-packaged in plastic mesh for easy recovery. Temperatures were taken manually. All samples were shipped on ice to the same lab as above. Laboratory analysis: Samples were dried in a convection oven for 24 hours. The lab analyzed the urushiol standard with an Agilent 6890N/5973N GCMS equipped with a DB-1 column. There was a very clear urushiol peak on the standard. Unfortunately when the extracts from the samples sent to the lab were analyzed, the peaks could not be separated. The main ions for urushiol were present all over and it was not clear whether the peaks were coming from the urushiol or from a common ion present in the material. Due to this, analysis of urushiol concentration in the samples was impossible. Further analysis attempts: The samples were picked up and sent back to CWMI in NY. These samples, as well as samples that had been stored in the freezer from days 16, 23, 56, 86 and 120 of the field trial and days 0, 1 and 41 of the bench trial were dried and ground. In addition, fresh poison ivy was collected and ground the same way (had not been stored). We ordered a product called SeeLeaf (SeeLeaf, Inc. is a Delaware corporation licensed and operating in Massachusetts). The SeeLeaf™ detection cloth immediately turns purple upon contact with the rash causing poison in these plants. Fresh poison ivy samples that were cut and applied to the SeeLeaf sheets did give a purple streak. Three grams of ground samples and 50 ml of 95% ethanol was put into individual beakers and allowed to soak for two days at room temperature. Extract from these samples was dropped onto SeeLeaf sheets with no color change (even from the fresh ground poison ivy that turned the sheets purple). Extraction was tried again using heat, but again, there was no color change. Conclusion: Unfortunately, we cannot make any conclusions from this research as we were unable to analyze the urushiol content. It is possible that the laboratory in San Diego and we in NY did not get any results because of the way it was handled. The literature indicates several ways in which urushiol loses its toxicity: (1) in a moist chamber the sap will completely lose its dermatitogenic activity within a week; moisture promotes degradation (2) urushiol is readily degraded in the presence of water (3) oxidation of urushiol decreases its toxicity - if the plaque is truly dry, it is no longer allergenic. As samples were stored in a freezer and put on ice, that would make a moist chamber, and when dried and ground, that gives time for oxidation of the urushiol.

Publications


    Progress 10/01/16 to 09/30/17

    Outputs
    Target Audience:Target Audience: General Public, over 90% of the general population is allergic to urushoil (the compound that cause dermitis), those who compost or otherwise manage poison Ivy or poison oak, compost facility managers. Changes/Problems:CWMI was awarded half of the money that was requested for the three year period, it can be spent over 2 years. The timing and the funds are really not enough to conduct the full scope, so we are doing as much as we can. This work is conducted in the environment and is growing season dependent in the northeast US. What opportunities for training and professional development has the project provided?There was one CCE technician that assisted with building compost piles, however, he was instructed to work with protection in the confines of the bucket loader as we did not want anyone else to be exposed to the urushiol. 2 people harvesting the poison ivy wore multiple layers of clothing which was fully covered with tyvek suits, face masks and breathing equipment for protection. Because of the nature of the work, it is not yet time for training and professional development. How have the results been disseminated to communities of interest?No results are yet available. The urushiol detection methods are being developed with mass sectrometry at San Diego State University. What do you plan to do during the next reporting period to accomplish the goals?Assess and analyze the organic material samples to determine when the urushiol breaks down to the point where it would not cause allergic reactions. If timing and funds allow we will conduct another trial and distribute the information.

    Impacts
    What was accomplished under these goals? Poisonous plants are often added to compost piles as a feedstock but we don't have information about their residual potency as the plant decomposes and becomes part of the soil medium. Residual toxins in plants might affect people working the composting pile and people using the compost. Contact with poison ivy (PI), Toxicodendron radicans, and oak (PO), Toxicodendron rydbergii, can cause an allergic response in 85% of the U.S. population (American Academy of Dermatology, 2015). Very little information is available on how to safely dispose of the plants (Rynk, 2000). A lot finds its way into home and centralized composting systems. Allergic reaction is caused by a compound called urushiol. Urushiol is present in the sap, roots, stems, leaves and fruit of the plant and released when plant is damaged by cutting, mowing, burning or touching. The oil is active on dead plants, clothing and objects for years. About 2 million cases of skin poisoning are reported annually. Question: do yard trimmings mixed with PI are disposed of in compost piles, will urushiol degrade or is it still active in finished compost? A number of heterotrophic bacteria, oil degrading and sulphate reducing bacteria, have been isolated in linseed oil (Pereria, M, et al., 2006). Decomposition research of urushiol will help to answer questions on plant disposal and its effect on ag workers, gardeners and others in contact with compost. Recommendations can be made on how to manage the disposal of PI/PO without affecting human health and also how to manage the composting process when these plants are present. Extension Objectives 1. The objective of this research is to assess the fate of the compound urushiol (the chemical in poison ivy and oak that causes skin irritation) in the composting process. 2. To develop research based recommendations for the disposal of poison ivy and oak and the resulting compost. Research Objectives Answer the question, "if plants containing urushiol are incorporated in compost windrows, will I have an allergic reaction to the compost?" Extensive literatures searches were completed on all aspects of urushiol, how the rash is contracted, and how the urushiol degrades and can be transmitted to others. Most of the research could not be started until poison ivy had fully emerged in late spring. June 12, 2017 research start date, arrived at the Education Center and 4-H Park in Otisville at 8 am. Scouted the grounds for poison ivy, Harvested 3, 1-yard buckets of poison ivy. Leaf and yard waste (partially composted) was delivered to the site at 10 am and unloaded in 2 piles. One sample of poison ivy alone was taken in a ½ pint glass jar and put on ice for Pile 2. Pile 2 was made at ~11:30 am: Approximately 1 bucket load of leaf and yard waste was laid as a base, 1 bucket load of PI was put on top; the pile was covered with woodchips and more leaf and yard waste to equal 2 bucket. The rest of the PI (about 1 bucket load) was left in a circle for Pile 3. Triplicate samples of each mix (pile 1, 2 and 3) were taken and put on ice for day 0. Temperatures were taken at 2 depths on all 4 sides of each pile. Put a fence around pile 3 and hung signage about the study and cautioning not to touch. Triplicate samples were taken from each pile and put on ice. Temperatures were taken at 2 depths on 4 sides of each pile. June 14, - Day 2; June 15 - Day 3, Triplicate samples were taken from each pile and put on ice. Temperatures were taken at 2 depths on 4 sides of each pile. Data loggers were placed in each pile. Logger 912861 will be used in Pile 3 - It has been set to record on 6/20/17 at noon and every 6 hours after that. Logger 912856 will be used in Piles 1 and 2 - Probe 4 should go in pile 2 and probe 1 should go in pile 1. It has been set to record on 6/20/17 at noon and every 6 hours after that. Robert Rynk professor at SUNY Cobleskill also conducted this work on a bench scale with two- 3 cu. ft. containers with forced aeration container 1 was filled with poison ivy and ground leaves. Container 2 was filled with poison ivy only. Temperatures were taken with data loggers. June 21- Day 9, June 28- Day 16, July 5- Day 23,Aug 9- Day 56,Sept 6- Day 86,Oct 10- Day 120. Triplicate samples were taken from each pile and put on ice. Samples were sent to Faith Buyuksonmez, 6259 Progressive Ave Suite 300, San Diego, CA 92154.

    Publications