Progress 09/01/16 to 09/30/20
Outputs
Target Audience:We provide service to Wyoming livestock producers, Wyoming pet owners, Wyoming Game and Fish Department. We also provide service to state and national public health organizations in testing for viruses with zoonotic potential. Our service includes diagnostic testing and surveillance testing to monitor for the presence or incursion of viruses of concern to public health and to the U.S. livestock industry. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project supported traing and AAVLD meeting attendance for our QC Manager. Attendance at this meeting has been very helpful to our QA/QC efforts, as much due to the personal contacts and interaction with QC managers from other labs as to the content of presentations. How have the results been disseminated to communities of interest?The Wyoming State Veterinary Laboroatory in collaboration with the USDA, APHIS and the Wyoming Livestock Board performs avian influenza virus (AIV) surveillance testing at Wyoming County fairs and the Wyoming State fair. This is an opportunity to educate the public and poultry exhibitors about the importance of biosecurity and disease surveillance in the prevention and control of important animal diseases such as AIV. At these venues we have presented posters describing the importance of AIV and how the laboratory processes their samples. Results of AIV testing is reported to the Livestock Board and to fair superintendents so the exhibitors receive the results of testing before they leave the fair. The WSVL has presented the purpose and importance of the NAHLN system to producer groups and practicing veterinarians around Wyoming. This includes annual diagnostic case presentations to the Wyoming Veterinary Medical Association. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
From 2016 to the present, the Wyoming State Veterinary Laboratory (WSVL) has had thirteen staff and faculty staff members complete a total of 102 proficiency tests under NAHLN, for such diseases asfoot and mouth disease virus, avian influenza virus, swine influenza virus, virulent (velogenic) Newcastle virus, classical swine fever virus and others. This represents a signifcant increase in both numbers of staff and numbers of PT tests completed, and was directly affected by support from this project. The breadth of tests and the number of staff available to perform them greatly increases the readiness of the WSVL to respond. Under this funding WSVL hasalso upgraded our LIMS system and instituted electronic messaging to NAHLN for AIV, with messaging for other tests in progress. As an American Association of Veterinary Laboratory Diagnosticians accredited lab and part of the NAHLN, the WSVL continues to performequine piroplasmosis testing in response to an outbreak in our state. Over the course of the grant, the WSVL has increased the number of technicians proficiency trained to test for viral disease of high impact to U.S. agriculture from 3 to 5. Testing capacity has also been increased by the addition of high throughput PCR instruments and automated nucleic acid extraction equipment. To maintain readiness for potential outbreaks, we have instituted a policy of having on hand two weeks worth of testing consumables for NAHLN tests. This includes plasticware, reagents and PPE. We have a plan for continuous rotation of most of these materials so we can be sure to test at capacity for two weeks without restocking or using expired or otherwise compromised materials.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Bangoura B, Bardsley KD. Ruminant Coccidiosis. Vet Clin North Am Food Anim
Pract. 2020 Mar;36(1):187-203. doi: 10.1016/j.cvfa.2019.12.006. PMID: 32029184.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Fischer D, Oberl�nder B, Peters M, Eley N, Pantchev N, Bangoura B, Lierz M.
Central nervous signs, blindness and cerebral vermicosis in free-ranging
peregrine falcons (Falco peregrinus) associated with aberrant larval migrations.
Vet Parasitol Reg Stud Reports. 2020 Apr;20:100410. doi:
10.1016/j.vprsr.2020.100410. Epub 2020 Apr 7. PMID: 32448538
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Fabian BT, Hedar F, Koethe M, Bangoura B, Maksimov P, Conraths FJ, Villena I,
Aubert D, Seeber F, Schares G. Fluorescent bead-based serological detection of
Toxoplasma gondii infection in chickens. Parasit Vectors. 2020 Jul 31;13(1):388.
doi: 10.1186/s13071-020-04244-6. PMID: 32736581; PMCID: PMC7393333.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Marin RE, Micheloud JF, Vignale ND, Gimeno EJ, O'Toole D, Gardner DR, Woods
L, Uzal FA. Intoxication by Astragalus garbancillo var.
garbancillo in llamas. J Vet Diagn Invest. 2020 May;32(3):467-470. doi:
10.1177/1040638720914338. Epub 2020 Apr 1. PMID: 32233843; PMCID: PMC7377608
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Elderbrook MJ, Schumaker BA, Ueti MW, Bastos de Almeida M, Vieira TSWJ,
Vieira RFC, Sondgeroth KS. Comparison of 2 ELISAs for detecting exposure to
Brucella ovis. J Vet Diagn Invest. 2020 Aug 4:1040638720943880. doi:
10.1177/1040638720943880. Epub ahead of print. PMID: 32748706
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Maloney M, Merkle JA, Aadland D, Peck D, Horan RD, Monteith KL, Winslow T,
Logan J, Finnoff D, Sims C, Schumaker B. Chronic wasting disease undermines
efforts to control the spread of brucellosis in the Greater Yellowstone
Ecosystem. Ecol Appl. 2020 Sep;30(6):e02129. doi: 10.1002/eap.2129. Epub 2020
May 5. PMID: 32223053
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Murray MH, Fidino M, Fyffe R, Byers KA, Pettengill JB, Sondgeroth KS, Killion
H, Magle SB, Rios MJ, Ortinau N, Santymire RM. City sanitation and
socioeconomics predict rat zoonotic infection across diverse neighbourhoods.
Zoonoses Public Health. 2020 Jun 25. doi: 10.1111/zph.12748. Epub ahead of
print. PMID: 32583624
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Progress 09/01/18 to 08/31/19
Outputs
Target Audience:The target audience and groups served by this project are livestock owners, wildlife managers and the Wyoming Game and Fish Department, and public health officials. Livestock owners include commercial and backyard poultry owners, cattle, sheep, and pig producers, as well as consumers and users of these agriculture products. Another important audience that greatly benefits from this project consists of the State of Wyoming wildlife managers and biologists for both avian and mammalian species. Public health officials and the citizens of Wyoming also obtain valuable information from this testing, as some of these diseases have zoonotic potential such as avian influenza. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Students and technical staff assisted in the IAV-A surveillance which is excellent experience for students especially. How have the results been disseminated to communities of interest?The status of the WSVL operations and testing, as well as disease outbreaks and related information is presented to the Wyoming veterinary Medical Association and the Wyoming Stock Growers Association twice a year at their summer and winter meetings. Other local meetings and events are also attended where NAHLN-related information is presented. What do you plan to do during the next reporting period to accomplish the goals?Weare about to receive final commisioning of our BSL3 space. As we begin to operate that space we will likely encounter unforeseen equipment and operational needs requiring NAHLN support. We will continue to support one technical staff member as well.
Impacts
What was accomplished under these goals?
1. Funds were used to support salary for one technical staff member in the WSVL. This individual supports work on NAHLN testing in bacteriology and virology. In addition, they are cross trained to free up other personnel in the lab for NAHLN testing. 2. We utilize NAHLN support to help with our IT requirements. Inthe past year it was used for aneeded upgrade to our document management system and support for messaging. NAHLN funds werealso used for server hosting expenses and maintenance contracts. 3. Supplies and small equipment to stand up BSL3 lab for diagnostics were obtained. Maintenance contracts for NAHLN required equipment including anABI thermocycler were supported. 4. Support to assist the State Veterinarian and APHIS in IAV-A surveillance at fairs in Wyoming.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Elderbrook M, Schumaker B, Cornish T, Peck D, Sondgeroth K.
Seroprevalence and risk factors of Brucella ovis in domestic sheep in Wyoming, USA.
BMC Vet Res. 2019 Jul 15;15(1):246
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Branscom LA, Cornish TE, Sondgeroth KS.
Evaluation of serologic testing of rams in the management of Brucella ovis in a domestic sheep flock.
J Vet Diagn Invest. 2019 Jan;31(1):86-89.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Pesavento PA, Cunha CW, Li H, Jackson K, O'Toole D.
In Situ Hybridization for Localization of Ovine Herpesvirus 2, the Agent of Sheep-Associated Malignant Catarrhal Fever, in Formalin-Fixed Tissues.
Vet Pathol. 2019 Jan;56(1):78-86.
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Progress 09/01/17 to 08/31/18
Outputs
Target Audience:We provide service to Wyoming livestock producers, Wyoming pet owners, Wyoming Game and Fish Department. We also provide service to state and national public health organizations in testing for viruses with zoonotic potential. Our service includes diagnostic testing and surveillance testing to monitor for the presence or incursion of viruses of concern to public health and to the U.S. livestock industry. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project supported traing and AAVLD meeting attendance for our QC Manager. Attendance at this meeting has been very helpful to our QA/QC efforts, as much due to the personal contacts and interaction with QC managers from other labs as to the content of presentations. How have the results been disseminated to communities of interest?The Wyoming State Veterinary Laboroatory in collaboration with the USDA, APHIS and the Wyoming Livestock Board performs avian influenza virus (AIV) surveillance testing at Wyoming County fairs and the Wyoming State fair. This is an opportunity to educate the public and poultry exhibitors about the importance of biosecurity and disease surveillance in the prevention and control of important animal diseases such as AIV. At these venues we have presented posters describing the importance of AIV and how the laboratory processes their samples. Results of AIV testing is reported to the Livestock Board and to fair superintendents so the exhibitors receive the results of testing before they leave the fair. The WSVL has presented the purpose and importance of the NAHLN system to producer groups and practicing veterinarians around Wyoming. This includes annual diagnostice case presentations to the Wyoming Veterinary Medical Association. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
From 2016 to the present, the Wyoming State Veterinary Laboratory (WSVL) has had thirteen staff and faculty staff members complete a total of 102 proficiency tests under NAHLN, for such diseases asfoot and mouth disease virus, avian influenza virus, swine influenza virus, virulent (velogenic) Newcastle virus, classical swine fever virus and others. This represents a signifcant increase in both numbers of staff and numbers of PT tests completed, and was directly affected by support from this project. The breadth of tests and the number of staff available to perform them greatly increases the readiness of the WSVL to respond. Under this funding WSVL hasalso upgraded our LIMS system and instituted electronic messaging to NAHLN for AIV, with messaging for other tests in progress. As an American Association of Veterinary Laboratory Diagnosticians accredited lab and part of the NAHLN, the WSVL continues to performequine piroplasmosis testing in response to an outbreak in our state. Over the course of the grant, the WSVL has increased the number of technicians proficiency trained to test for viral disease of high impact to U.S. agriculture from 3 to 5. Testing capacity has also been increased by the addition of high throughput PCR instruments and automated nucleic acid extraction equipment. To maintain readiness for potential outbreaks, we have instituted a policy of having on hand two weeks worth of testing consumables for NAHLN tests. This includes plasticwear, reagents and PPE. We have a plan for continuous rotation of most of these materials so we can be sure to test at capacity for two weeks without restocking or using expired or otherwise compromised materials.
Publications
|
Progress 09/01/16 to 08/31/17
Outputs
Target Audience:We provide service to Wyoming livestock producers, Wyoming pet owners, Wyoming Game and Fish Department. We also provide service to state and national public health organizations in testing for viruses with zoonotic potential. Our service includes diagnostic testing and surveillance testing to monitor for the presence or incursion of viruses of concern to public health and to the U.S. livestock industry. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?The Wyoming State Veterinary Laboroatory in collaboration with the USDA, APHIS and the Wyoming Livestock Board performs avian influenza virus (AIV) surveillance testing at Wyoming County fairs and the Wyoming State fair. This is an opportunity to educate the public and poultry exhibitors about the importance of biosecurity and disease surveillance in the prevention and control of important animal diseases such as AIV. At these venues we have presented posters describing the importance of AIV and how the laboratory processes their samples. Results of AIV testing is reported to the Livestock Board and to fair superintendents so the exhibitors receive the results of testing before they leave the fair. The WSVL has presented the purpose and importance of the NAHLN system to producer groups and practicing veterinarians around Wyoming. This includes annual diagnostic case presentations to the Wyoming Veterinary Medical Association. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The Wyoming State Veterinary Laboratory (WSVL) has five staff members proficiency trained and certified to test for foot and mouth disease virus, avian influenza virus, swine influenza virus, virulent (velogenic) Newcastle virus, classical swine fever virus. Three staff are proficiency trained and certified to test for vesicular stomatitis virus. As an American Association of Veterinary Laboratory Diagnosticians accredited lab and part of the NAHLN, the WSVL assisted in the national response to epizootics of highly pathogenic avian influenza virus and vesicular stomatitis virus in 2015. Over the course of the grant, the WSVL has increased the number of technicians proficiency trained to test for viral disease of high impact to U.S. agriculture from 3 to 5. Testing capacity has also been increased by the addition of high throughput PCR instruments and automated nucleic acid extraction equipment.
Publications
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