Source: CORNELL UNIVERSITY submitted to
FLY MANAGEMENT IN ANIMAL AGRICULTURE SYSTEMS AND IMPACTS ON ANIMAL HEALTH AND FOOD SAFETY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1010365
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
S-1060
Project Start Date
Oct 1, 2016
Project End Date
Oct 2, 2016
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Project Director
Scott, JE, G.
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Entomology
Non Technical Summary
House flies are major pests of animal production facilities because they are capable of transmitting scores of diseases to humans and animals. Pyrethroid insecticides are the primary insecticides used for control of flies in and around animal production facilities, thus mitigating resistance is of paramount importance. We will investigate the fitness costs of three important resistance alleles.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
72134101080100%
Knowledge Area
721 - Insects and Other Pests Affecting Humans;

Subject Of Investigation
3410 - Dairy cattle, live animal;

Field Of Science
1080 - Genetics;
Goals / Objectives
Insecticide resistance detection and management
Project Methods
Objective 1. Individual unmated house flies from the appropriate strain will have one leg removed to allow for genotyping of the Vssc allele(s) present. House flies of the appropriate genotype will be pooled to start individual strains having only one Vssc allele. These strains will be individually crossed to the susceptible strain, backcrossed and flies containing the appropriate markers will be crossed. Flies from the subsequent generation will be genotyped to isolate strains having only the the Vssc allele of interest in the background of the susceptible strain.Objective 2. We will cross the above strains to produce a starting population that has 25% of each allele (susceptible, kdr, kdr-his and super-kdr).Objective 3. Four replicate cages will be used for each environmental condition: normal laboratory conditions, cold stress (4° for 2 days as pupae) or heat stress (32°C for 2 days as pupae). Adult flies (50 from each replicate) will be genotyped every generation to determine the alleles present.Objective 4. Knowing the relative fitness costs of each resistance allele under different environmental conditions will be important for understanding how rapidly we would expect to see reversion of resistance in the absence of insecticide use.

Progress 10/01/16 to 10/02/16

Outputs
Target Audience:This project was established in error. Changes/Problems:This project was established in error. What opportunities for training and professional development has the project provided?This project was established in error. How have the results been disseminated to communities of interest?This project was established in error. What do you plan to do during the next reporting period to accomplish the goals?This project was established in error.

Impacts
What was accomplished under these goals? This project was established in error.

Publications