Source: COLD SPRING HARBOR LABORATORY ASSOCIATION, INC submitted to NRP
COLD SPRING HARBOR LABORATORY WORKSHOP ON CEREAL GENOMICS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1010253
Grant No.
2017-67013-25921
Cumulative Award Amt.
$25,000.00
Proposal No.
2016-07092
Multistate No.
(N/A)
Project Start Date
Nov 1, 2016
Project End Date
Oct 31, 2017
Grant Year
2017
Program Code
[A1141]- Plant Health and Production and Plant Products: Plant Breeding for Agricultural Production
Recipient Organization
COLD SPRING HARBOR LABORATORY ASSOCIATION, INC
1 BUNGTOWN RD
COLD SPRING HARBOR,NY 11724-2209
Performing Department
Meetings and Courses
Non Technical Summary
This workshop in Cereal Genomics enables participants to take advantage of emerging genetic tools and the completed cereal genome sequences of most of the major cereal crops. The workshop features lectures and lab exercises, including hands-on lab work and computer sessions in comparative anatomy, GWAS, Next Gen expression analysis, whole genome sequencing assembly, emerging model systems, genome editing and phenomics. The faculty (instructors and invited lecturers) are active researchers in cereal genetics and genomics who have made significant contributions to the field, ensuring that the latest techniques and ideas will be presented. The course is structured to provide time for informal discussions and exchange with instructors.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20115491080100%
Keywords
Goals / Objectives
Cereal Genomics Workshop at Cold Spring Harbor Lab.Dave Jackson, Doreen Ware, Sarah Hake, April 2016.This proposal requests funds to support the participation of US graduate students in plant genetics and breeding to a training course in cereal genomics. A major aim of this course will be to enable students and post docs to take advantage of the emerging genomic data in the cereals, including complete genome sequences of most cereal crops and model systems, and become trained in methods including Next Gen gene expression analysis, genome wide association analysis and genome editing. The proposal is targeted at the "Plant Breeding for Agricultural Production" priority area- since it will train students in genomics techniques that can enable plant breeding. Specific parts of the workshop are devoted to quantitative genetics, genome analysis, genome editing and phenomics, major areas of interest to current and future breeding efforts. It will also enable graduate student interaction with geneticists and other scientists, to promote careers in plant breeding, genetics and genomics. As a follow up to the highly successful Cereal Genomics workshops held in 2005 and 2009 at CSHL, we propose to organize this week-long workshop in the fall of 2016. With the recent releases of many cereal genome sequences, availability of huge GWAS and phenomics datasets and the emerging revolutionary genome editing technologies, the time is ripe to train young students and scientists in the tools and skills they need to exploit the emerging genome data across all cereal crops. The workshop is geared toward scientists from graduate, post doctoral, faculty and industry levels, and will teach state of the art methodologies in cereal crop genomic analysis and data processing. Topics will include Cereal Anatomy and Diversity, Molecular Breeding, QTL/ GWAS, Genome Sequencing and Structure, mRNAseq Analysis, Genome Editing and Databases. The format will be morning and evening lectures and afternoon hands on labs in informatics, using resources at CSHL and Cyverse. It is an ideal time for a new workshop, because of the recent advances in whole genome sequencing, genome editing and related fields. In addition, the general trend in funding geared towards crop plants means that many labs are trying to re-tool. This workshop will serve as a catalyst and important training forum to disseminate new techniques and promote use of data from other federally funded projects.
Project Methods
CEREAL GENOMICS WORKSHOP OUTLINE.The workshop is being organized by a core group of three workshop directors, who will attend the whole workshop to ensure continuity and provide support where needed:Dave Jackson, Professor, Cold Spring Harbor Lab., NY 11724.Sarah Hake, Director, Plant Gene Expression Center, Albany, CA 94710; Full Adjunct Professor, Plant and Microbial Biology Dept., University of California, Berkeley.Doreen Ware, Associate Professor, Cold Spring Harbor Lab., NY 11724.The workshop will run on a template of the existing, highly successful CSHL courses. The short duration of the workshop and its contemporary content makes it both attractive and valuable to established investigators as well as to advanced graduate students and postdoctoral fellows. The three core workshop directors will attend the entire workshop to ensure continuity between the different presentations. Approximately 12 invited lecturers will give lectures on topics in cereal biology, genetics and genomics, combined with practical computer sessions that reinforce the lectures by emphasizing problem solving and skill development. A typical day will consist of two 2-hour lectures delivered at 9:00 AM and 7:00 PM. The afternoons will consist of a 4-5 hour "hands on" sessions that will be a combination of "wet lab" (observing phenotypes, collecting data) and computer sessions centered around problem sets designed and supervised one of the days' lecturers. The problem sets will present students with typical tasks in cereal genome data manipulation. After the completion of the problem set, the lecturer will meet with the students to review the problems and to discuss any unresolved issues.This workshop will provide both "wet lab" and in silico training, and the lab will be set up so that each student has a laptop. "Wet lab" training will be provided in the comparative anatomy, phenomics and QTL sections, where students will examine samples and make measurements for statistical analysis. The faculty (instructors and lecturers- see list, below) represent active researchers in cereal genetics and genomics who have made significant contributions to the field. This will ensure that the newest techniques and ideas are presented. The seminar schedule will also be circulated throughout Cold Spring Harbor Laboratory to facilitate attendance by CSHL scientists, thereby increasing the interaction between workshop participants and the resident staff.Students will be housed on-site, and all amenities provided. Each student will pay a registration fee (to be determined), and some will be eligible to apply for partial scholarships. It is expected that the students will represent the international cereal genetics community, though more than half will be from the USA. Specific recruitment efforts will be made to involve students from under-represented groups. Lecturers will stay at Cold Spring Harbor for at least one full day, and usually for two, to answer any questions that the students may have. Lecturers and workshop leaders will be available during laboratories for questions and spontaneous discussion, and the computer laboratory will remain open throughout the night in order to accommodate students who wish to continue working after midnight.The list of proposed lectures and classes, as detailed below, has been assembled by the workshop directors. The lecturers have confirmed their attendance and enthusiasm to be a part of the workshop. We have chosen the lecturers to have a balance of male and female lecturers and include some who are at an early stage in their career. They also cover all the major cereal crops and model systems, including maize, rice, wheat, sorghum, brachypodium and setaria. A finalized schedule will be prepared well in advance of the workshop and will be given to students along with experimental protocols and lists of relevant papers before they arrive for the workshop.

Progress 11/01/16 to 10/31/17

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The students were trained in modern genomics techniques relevant to plant breeding and crop improvement. The small format of the workshop made it easy for students to network with the instructors, and discussions continued throughout mealtimes and after the lecture, at the bar. Students gained in depth understanding of bioinformatics methods, and all data from the workshop, and workflows are permanently stored on Cyverse. Effectiveness of the workshop was assessed using a survey, filled out by for students. The responses were unanimously very positive ("exceptional" or "very good"), with statements such as: "The organizers should be congratulated on a brilliant, well organized and intense course, .... wonderful course, wonderful instructors... diversity of students was beneficial for discussions,.... I learned so much, and the experience was invaluable,.... I learned many tools that will greatly benefit my work, .... The workshop was a truly an amazing experience that has already benefited my research". From past experience, we find that students who have attended these workshops gain confidence and self-motivation, and a close bond with other students and instructors, important for future careers and networking. We believe that this workshop was a great success, and are planning a follow-up workshop in 2019. How have the results been disseminated to communities of interest?The workshop materials are freely available through Cyverse, for anyone who has a (free) CyVerse account in the Discovery Environment), URL: /iplant/home/shared/iplant_training/cereal_genomics_CSHL2016 What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? A highly successful Workshop on Cereal Genomics was held at Cold Spring Harbor Laboratory from October 19 - October 25, 2016. The lead instructors were Sarah Hake, University of California, Berkeley, Albany, CA, David Jackson, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY and Doreen Ware, USDA/ARS and Cold Spring Harbor Laboratory. The workshop trained 17 participants (graduate students or post docs, and we had balanced representation, with 8 Males and 9 Females, including 2 underrepresented minorities. Fourteen of the students were from the US, and 3 from overseas (Australia, China). Nine of the US students benefitted from USDA Scholarship Funding. The workshop covered the following topics, with lectures and hands on bioinformatics classes led by invited instructors, listed below. Instruction included a range of cereals, including maize, rice, wheat, sorghum, orphan cereal crops, brachypodium and setaria. Comparative anatomy and phylogeny; Cereal genomes, assembly, annotation and synteny; Genetics and databases; Quantitative trait locus mapping and genome wide association studies; Genome wide expression analyses; Reverse genetics and genome editing; Phenomics PARTICIPANTS: *Martin Alexander, Ph.D., Graduate Student, University of California, Berkeley, CA. Lab Head: Dr. Sarah Hake *Demeke Bayable, MSc, Graduate Student, Purdue University, West Lafayette, IN. Lab Head: Dr. Tesfaye Mengiste Andrew Bowerman, Ph.D., Post Doctoral Fellow, The Australian National University, AUSTRALIA. Lab Head: Dr. Barry Pogson Jennifer Brophy, Ph.D., Post Doctoral Fellow, Massachusetts Institute of Technology, Cambridge, MA. Lab Head: Dr. Christopher Voigt *Katherine Guthrie, Graduate Student, University of Missouri Columbia, MO. Lab Head: Dr. Paula McSteen *Brianna Haining, Graduate Student, University of California, Berkeley, CA. Lab Head: Dr. Sarah C. Hake *Harry Klein, Ph.D., Graduate Student, University of Massachusetts, Amherst, MA. Lab Head:Dr. Madelaine E. Bartlett *Stephanie Klein, Graduate Student, The Pennsylvania State University, University Park, PA. Lab Head: Dr. Jonathan Lynch Katie Liberatore, Ph.D., Post Doctoral Fellow, United States Department of Agriculture- Agricultural Research Service, St. Paul, MN. Lab Head: Dr. Shahryar Kianian Haijun Liu, Graduate Student, Huazhong Agricultural University, CHINA. Lab Head: Dr. Yan Jianbing *Elly Poretsky, Graduate Student, University of California, San Diego, CA. Lab Head: Dr. Alisa Huffaker Candace Seeve, Ph.D., Post Doctoral Fellow, United States Department of Agriculture- Agricultural Research Service, Columbia, MO. Lab Head: Dr. Melvin Oliver Xiaomeng Shen, Graduate Student, HuaZhong Agriculture Universiy, CHINA. Lab Head: Dr. Qifa Zhang *Ashley Smith, Graduate Student, University of Wisconsin, Milwaukee, WI. Lab Head: Dr. Dazhong Zhao Karyn Willyerd, Ph.D., Post Doctoral Fellow, Oklahoma State University, Stillwater, OK. Lab Head: Dr. Charles Chen *Kameron Wittmeyer, Ph.D., Graduate Student, Pennsylvania State University, University Park, PA. Lab Head: Dr. Surinder Chopra Chuanmei Zhu, Ph.D., Post Doctoral Fellow, Donald Danforth Plant Science Center, St. Louis, MO. Lab Head: Dr. Elizabeth Kellogg * USDA Scholarship support. Invited instructors: Edward Buckler, United States Department of Agriculture- Agricultural Research Service , Ithaca, NY "Breeding 4.0: Sorting through the adaptive and deleterious variants across the maize genome" Uta Paszkowski, University of Cambridge, Cambridge, United Kingdom "Genetics and genomics of endosymbiosis: fundamental science and translation" Devin Connor, University of Cambridge, Cambridge, United Kingdom "Brachypodium tools and their application to comparative developmental biology" Zhanguo Xin, United States Department of Agriculture- Agricultural Research Service, Lubbock, TX "Pedigree Mutant Library as a resource for forward and reverse genetics in sorghum" Patrick Brown, University of Illinois, Urbana, IL "Using sorghum to understand and improve stress resilience in cereals" Katrien Devos, University of Georgia, Augusta, GA "Use of genotyping-by-sequencing in the genomic analyses of non-model plants: from inbreeding diploids to outcrossing polyploids" Christopher Topp, Danforth Plan Science Center, Saint Louis, MO "Reality is a perception: using phenomics to explore the hidden world of plant phenotypes" Caixia Gao, Institute of Genomics and Developmental Biology, Beijing, China "Genome editing in wheat and corn" Matthew Hudson, University of Illinois, Urbana, IL "Extracting biological insight from gene expression data" "RNAseq methods and data analysis in complex genomes: dealing with uncertainty, alternative splicing" Andrea Eveland, Danforth Plant Science Center, Saint Louis, MO "Extracting biological insight from gene expression data" Lisa Harper, University of California Berkeley, Berkeley, CA "Cereals and their Genomes" Elizabeth Kellogg, University of Missouri Saint Louis, Saint Louis, MO "Introduction to the grasses and major sub-groups" "Evolution of ecologically dominant grasses" Doreen Ware, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY "Genome Assembly"

Publications