Source: UNIV OF MINNESOTA submitted to NRP
TURKEY NUTRITION, MANAGEMENT AND WELL-BEING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1010118
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2016
Project End Date
Sep 30, 2021
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF MINNESOTA
(N/A)
ST PAUL,MN 55108
Performing Department
Animal Science
Non Technical Summary
Poultry nutrition, management and well-being are now linked together to an even greater extent than before due to rapid changes in decreased antibiotic use in the feed and consumer driven expectations. The nutritionist will need to sort through the myriad of products (enzymes, probiotics, etc.) to replace antibiotic use. An additional challenge for turkey production is the lack of updated nutrient requirements. Previous work has indicated that the lysine requirement of young turkeys has increased substantially, an indication that requirements of other amino acids will be increased as well. Increasing the protein content of the diet can potentially stress gut health and litter condition affecting bird welfare through development of dermatitis-type conditions associated with the skin. Biosecurity enhancements are needed to reduce disease challenges. A series of studies are proposed to determine amino acid requirements for market turkeys and examine linkages with management and bird well-being.
Animal Health Component
90%
Research Effort Categories
Basic
10%
Applied
90%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3023299101070%
3153299101015%
3073299116015%
Goals / Objectives
Determine the dietary lysine requirement for market turkeys at different agesDetermine the sensitivity in diet amino acid ratios for market turkeys at different agesBased on results from Objectives 1 and 2, test the feeding programs for adequacy at different dietary energy levelsDetermine efficacy of biosecurity implementation and litter management to decrease disease transmission and footpad incidence and severity Determine performance of turkeys fed diets containing antibiotic alternatives, high quality/alternative ingredients or with varying diet nutrient density.Within studies for the objectives above, assess development of the gut, microbial community and health status of birds.Within studies for the objectives above, determine the influence of the feed/management on turkey behavior, litter quality, litter moisture and dermatitis- type conditions associated with the skin
Project Methods
For objective 1, the lysine requirement will be determined in turkeys during the following age periods (starter, grower and finisher stages). A basal summit approach will be used to set up the experimental diets varying in lysine content. The basal and summit diets are to contain the same ratio of amino acid content to lysine with the basal diet representing the lowest level of lysine fed and the summit diet containing the highest content of lysine. Five additional diets with increasing lysine will be formed by mixing differing proportions of the basal and summit diets resulting in a total of seven diets with varying lysine content. Measures of performance include body weight, feed intake, and for the oldest turkeys, carcass and parts yield. Statistical analyses include ANOVA and use of different regression models to determine the estimated lysine requirement. For objective 2, the sensitivity of response to changes in amino acid ratio will be examined in turkeys during the starter, grower and finisher stages. . Recommended amino acid ratios will be used as the control. For the test diets, the ratio will be varied one amino acid at a time by creating a diet where the ratio is lower and higher than the control diet. A diet slightly deficient in lysine will be used to assure that lysine is not in excess. The targeted amino acids for study are total sulfur amino acids (TSAA), threonine (thr), arginine (arg) and valine (val). Measures of performance include body weight, feed intake, and for the oldest turkeys, carcass and parts yield. Statistical analyses include ANOVA, contrast testing and mean comparisons.For objective 3, the estimated lysine requirement (from Objective 1) along with any needed revisions in amino acid ratio (from Objective 2) will be used to the set the dietary lysine level as the positive control. Test diets will have the lysine levels decreased and increased by approximately 10% (depending on confidence interval determined in Objective 1). In a factorial manner the different lysine feed regimes (lower, adequate, higher) will be combined with diet metabolizable energy content (lower, adequate, higher). Measures of performance include body weight, feed intake, and carcass and parts yield. Statistical analyses include ANOVA contrast testing and mean comparisons.For objective 4, risks associated with litter- related health issues such as contact dermatitis (Collett, 2012) will be examined by replacing portions of the litter with alternative flooring (slotted or slatted) that allows excreta to be collected separately (Noll et al, 1997). Performance will be assessed along with carcass lesions. Turkeys will be assessed for behavior relative to time spent on the litter vs the slotted flooring. Use of slotted flooring is also a biosecurity measure as we hypothesize litter management will be reduced and bedding will remain in better shape for longer periods of time. Additional biosecurity measures to be used include a Danish Entry (a method used by the swine industry to minimize contamination of barn entryways), extension of concrete aprons for the buildings to keep entrances clean; and, cleaning and disinfecting vehicles.For objective 5, positive and negative control diets will be individualized for feed ingredient or feed additive testing. Depending on the study, a factorial arrangement could be used or treatments can focus on a single additive. The planned studies include a comparison of different combinations of enzymes and feed additives to determine their ability to replace growth promotion type antibiotics in the feed. The other area of interest is the investigation of specialized feed ingredients that are highly digestible and are applicable to use in early starting diets. Measures of performance include gut development, body weight, and feed intake. Statistical analyses include ANOVA, contrast testing and mean comparisons.For objective 6 and selected studies, the general approach is to euthanize a sub-sample of turkeys at various ages, isolate the digestive tract, measure length and weight of parts of the tract and collect gut contents from the tract and cecum. The gut contents will undergo DNA extraction and bacterial community profiling (16S rRNA). The data collected will be analyzed and compared among the treatment groups (Danziesen et al, 2013).For objective 7 and selected studies, the general approach is to utilize a scoring system for the litter and dermatitis-type conditions on the bird (foot pad, hock and/or breast area). Foot pads tend to have the majority of dermatitis lesions (Allain et al. 2013; DaCosta et al., 2013). For foot pad scoring, both foot pads of individual turkeys will be scored using the methodology provided by Clark et al. (2002) or the 5?Step® Animal Welfare Rating Standards for Turkeys (Issued April 27, 2015, v2.0, ©2015 Global Animal Partnership). Both scoring systems use an increasing number score based on increased severity related to the involved surface area of the foot pad. For litter moisture, samples are collected, mixed and subsample analyzed for litter moisture. Litter moisture will be determined by drying 50 g samples in a forced ventilation oven at a temperature of 60º C for 24 h (Fairchild and Czarick, 2011) with duplicate samples. Minerals will be determined by Inductively Coupled Plasma Mass Spectrometry (ICP) at a commercial laboratory. At the same time, ammonia and nitrogen by combustion will be determined. Litter scores will consist of two measures - one for dryness using a scale of 1 to 10 (deJong et al 2014) and the percentage of caked litter in the floor area of measurement (Grimes et al., 2006). Litter scores will be determined for each quarter of the pen. As litter characteristics can vary substantially within a pen or barn, behavior studies will be conducted to determine time budgets and preference for resting location on the litter bed.For the previously described studies, an approach is outlined. However, the actual approach(es) may vary as studies are initiated and results are obtained that may indicate a deviation from what has been described in order to successfully complete the stated objectives.

Progress 10/01/16 to 09/30/21

Outputs
Target Audience:The target audience includes poultry scientists, turkey producers that produce their own turkey feed; commercial poultry nutritionists and consultants; poutlry feed industry; and amino acid suppliers. Owners and youth working with small flocks. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Undergraduate and graduate students received training on feed analyses, bird care, data collection, data entry, data summarization and preparation of report tables and graphs. Two of the students gained experience writing an abstract for presentation at the annual summer meeting of the Poultry Science association and preparing their presentation in front of a national audience. . How have the results been disseminated to communities of interest?Through industry meetings in the midwest and national meetings such as the Poultry Science Association, and Nutrition Conferences - Minnesota Nutrition conference. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? For Goals 1 and 2, research results indicated an updating of amino acid requirements and ratios are needed for older market turkeys. The research emphasized amino acid nutrition of market turkeys and soybean meal as it provides the majority of crude protein and lysine in turkey diets while also contributing anti-nutritional factors, which need to be considered in feed formulation. Available amino acid requirement data is very outdated with sparse new information especially for older turkeys. A series of feeding trials determined the digestible lysine requirement of market turkeys and ideal protein ratio at different ages. The digestible lysine requirement differed from available recommendations as bird age increased after 8 weeks of age. At 16 wks of age, the estimated digestible lysine requirement of 1.07% was 1.5x greater than the NRC (1994) estimated digestible lysine requirement. Many poultry nutritionists have moved toward using ideal protein ratio, expressed as the ratio of digestible amino acid to digestible lysine in formulating diets. A sensitivity test was conducted using existing ratio information with lower and higher levels of key digestible amino acids to determine if the existing ratio was adequate to use in feed formulation in market turkeys based on weight gain and feed conversion. In young poults, the more appropriate ratio for arginine was 1.06 vs 1.01. In growing turkeys, a higher ratio was noted for valine (.74), which was similar to the finding in older market turkeys, where the more appropriate ratio for valine was .75 compared to .7. For Goal 5, specialty products derived from soybean were found to improve turkey performance. These products had lower levels of anti-nutritional factors and in some cases had immune-stimulating components included. Metabolizable energy content of the new product determined with young turkeys was found to increase with removal of the oligosaccharides from the meal. To help utilization of typical soybean meal especially in an antibiotic free, vegetable-based feeding program, alpha-galactosidase addition to the feed was assessed. Enzyme addition improved turkey performance to 9 weeks of age. Litter moisture in pens of turkeys fed high levels of soybean meal in the diet resulted in greater litter moisture suggesting a higher level of litter management is needed in AV feed program. Enzyme supplementation was beneficial in young bird diets when soybean meal level was at their highest. The interplay of research in Goals 1, 2 and 5 was examined under the conditions of providing additional nutrients in early starting diets as well exploring delayed placement of turkey poults after hatching on performance during brooding and influence on final market body weights. Increased density of protein, lysine and threonine were more consistent in having a positive impact on market weight as well as at the end of brood. Delayed placement of 48 hrs post hatch resulted in decreased weights at 6 wks of age. For Goal 4, biosecurity compliance was explored in commercial and small flock settings. Small flocks are more likely to move birds from the farm especially for competition. A survey of biosecurity practices of 4-H exhibitors was conducted at the Minnesota State Fair. Some deficiencies were found relative to incomplete knowledge of quarantine and avian respiratory diseases. This subsequently led to an effort to educate small flock owners of respiratory diseases, diagnostics and biosecurity.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: A. Neu, K. Lautenschlager, S. Voss, S. Noll, and D. Lauer. 2018 Preparing poultry producers to develop an effective biosecurity plan.Poult. Sci. 97(E-Suppl . 1):178
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: SL Noll, Z. Jiang,PB Tillman. 2016. Digestible amino acid ratios for starting and finishing male market turkeys. Poult. Sci. 95(E-Suppl. 1) p138
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Cortus, E., Neu, A.E., Janni, K.A., Noll, S.L., Clanton, C.J. 2019. Simulating risk reduction using biosecurity practices on farm. ASABE Paper No. 1900870. St. Joseph, MI. ASABE.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: E. Theis, W. Martin, SL Noll, R. Porter, C. Carlson, M. Olson, Abby Neu-Schuft. 2021. An online webinar series to teach backyard flock owners about poultry viral respiratory diseases.Poult. Sci. 100 (E-Suppl 1): 14.


Progress 10/01/19 to 09/30/20

Outputs
Target Audience:The targeted audience for this work are turkey producers, poultry nutritionists, and retailers with interest in selling meat products produced from turkeys fed all vegetable-based feed. Changes/Problems:Gut contents were collected for microbiome analyses in both studies and results are delayed due to COVID restricting laboratory access. What opportunities for training and professional development has the project provided?A research associate was trained in the methodology of metabolizable energy determination in poultry and in design, animal care, sample collection, and data analyses. Undergraduate students (3) were trained in bird care, sample collection/processing/analyses; data handling, analyses and data summarization. How have the results been disseminated to communities of interest?Results have been disseminated through regional publications and regional/national conference presentations targeted to turkey producers and poultry nutritionists; informal meetings with poultry nutritionists and processors. What do you plan to do during the next reporting period to accomplish the goals?Complete remaining laboratory work on gut microbiome analyses.

Impacts
What was accomplished under these goals? For Goals 5 and 7, the role of soybean meal in turkey performance and litter conditions was examined. Soybean meal (SBM) is a high protein feed ingredient critical to meeting the dietary protein and amino acid needs of commercial turkeys which have an inherently high requirement compared to other poultry. Since large quantities of SBM are needed in the turkey diet, characterization and knowledge of the nutrient characteristics and contribution of anti-nutritional components is needed for effective use. The reported studies demonstrated that 1) In young turkeys, that SBM from aged seeds resulted in reduced performance and, 2) in a feeding trial to market age, diets with SBM as the primary source of protein, resulted in poorer growth and feed conversion in younger turkeys. Further study is needed to better define nutrient characteristics and approaches to maintain young turkey growth in corn-soybean meal diets. One study was conducted to test the nutritional value of SBM produced from beans varying in seed size, growing condition (shade), storage (aged), protein, and oleic acid content. Comparisons were made to a commodity soybean meal and the control soybean material. Amino acid content (total and digestible) were determined. The live bird tests included determination of apparent metabolizable energy (AME), a young turkey growth assay which included measuring the impact on excreta moisture and growth of gut associated organs and tissues. Diets contained 44% of the sourced soybean meal. Lysine digestibility coefficients were similar for the various meals and ranged from 85 to 88%. No clear distinctions were observed among the different meals for organ or gut growth. The lowest AME content was observed for the meal derived from seed grown in shade. Depending on the control group for comparison, approximately 30% less energy was observed. Meals derived from high protein, large seed, small seed, or beans grown in shade resulted in heavier body weights at 2 wks of age as compared to the remaining bean types while poults fed meal from aged seeds tended to have excreta that was wet and with poorer growth. An area for exploration in the future would be to assess the impact and length of storage on the energy content and nutritional value. The second study examined feeding corn-soybean meal based diets to commercial turkeys from hatch to market age. For certain consumer markets, retailers want meat generated from animals fed diets without animal byproduct inclusion. These products contribute significant amounts of protein, minerals, and vitamins to the diet. Because turkeys have such a high requirement for amino acids, there are few plant protein sources with sufficient amino acid concentration to replace meat meal. Soybean meal (SBM) is readily available and is the most concentrated source of plant protein. However, SBM has some negatives when feeding to high inclusion levels that might be needed in all vegetable type diets. These include difficulties in supplying energy (higher supplemental fat level needed), and presence of indigestible fibers, high potassium, phytate and other antinutritional factors. These various issues impact bird performance, footpad dermatitis, gut health and litter characteristics. It is anticipated that the negative impact of the high inclusion levels of SBM will be exacerbated in antibiotic free (ABF) production systems. Tools that could help negate the high SBM level plant based diets would be to use appropriate enzymes to increase energy and nutrient digestibility or increase use of amino acid supplements (lysine, methionine, and threonine). The project goal was to assess available tools for all-vegetable feed program (AV) using high soybean meal type diets. Turkey tom performance and litter condition were assessed. Male turkeys were reared to 18 wks of age and fed diets formulated with varying levels of SBM. The test diets were supplemented with and without enzyme (alpha-galactosidase). The test diets were: Conventional (C) with meat meal and BMD (Bacitracin) (T1); Corn-SBM (AV) and BMD (T2); As T2, no BMD (T3); As T3 with staggered reductions of SBM and increased amino acid supplementation (T4, T5). Results obtained indicated the following significant results (P<.05): 1) AV feed program resulted in reduced turkey weight through 9 wks of age in comparison to T1; 2) Supplementation of BMD in AV resulted in similar performance of T2 (AV) with T1 (C); 3) Increasing amino acid supplementation while reducing diet soybean level resulted in decreased weight; 4) Enzyme supplementation to 9 wks resulted in similar feed conversion and body weight; 5) Litter moisture was correlated with SBM such that higher SBM resulted in more moisture in the litter. In conclusion, an AV feed program results in both formulation and performance challenges. Ingredient cost per ton could be reduced with enzyme use and/or reduction in the diet level of SBM however those costs need to be balanced with performance. Use of BMD was beneficial in the AV diet but will not be present in ABF programs to moderate the lowered growth response when coupled with AV feeding. A higher level of litter management will be needed in AV feed programs.

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2020 Citation: Noll, S. L., 2020. Reducing the negative impact of high inclusion levels of soybean meal in all vegetable type diets for turkeys. Presented at virtual 81st Minnesota Nutrition Conference. Minnesota Extension Service.
  • Type: Other Status: Published Year Published: 2020 Citation: Noll, S.L., J. Brannon, S. Naeve, T. Johnson. 2020. Soybean composition impact on turkey gut health and nutrition. Gobbles (July), Vol. 77, No. 5. Pg 5
  • Type: Other Status: Published Year Published: 2020 Citation: Noll, S. L., J. Brannon, T. J. Johnson, 2020. Reducing the negative impact of high inclusion levels of soybean meal in all vegetable type diets for turkeys. Gobbles (November), Vol. 77, No. 9. Pg 10


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:The target audience includes soybean meal processing companies, poultry nutritionists, consultants, feed industry and producers of commercial turkeys. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Undergraduate and graduate students received training in feed analyses, care of poultry, collection of data, data entry and quality checks, data summarization and preparation of report tables. Involved in the project were four undergraduate students - one animal science, one pre-medical and two pre-veterinary students; and one graduate student. How have the results been disseminated to communities of interest?Initial results were presented at a fall conference (2018, reported previously). Results were also reported to the Minnesota Soybean Research group. What do you plan to do during the next reporting period to accomplish the goals?Research has been initiated in relation to Goal #5 regarding the impact of different types of soybean meal varying in genetics and growing conditions in relation to metabolizale energy content and young turkey performance.

Impacts
What was accomplished under these goals? Research emphasis focused on Goal #5 (Determine performance of turkeys fed diets containing ... high quality/alternative ingredients). For most commercial meat poultry, soybean meal remains the main contributor of quality protein and amino acids in the feed. However, other portions of the ingredient contain factors which can have negative impact on bird performance and gut health. Newer processing methods has allowed development of a high protein material with less antinutritional factors present. The specially processed soybean meal material was tested as an additive in starting turkey diets with performance followed through to market weight. The material (NutriVanceTM, Midwest Ag Enterprises, Inc) was included at levels of 2.5 and 5%, or incorporated with other speciality protein products identified as Fermotide and Neptide and fed at 2.5% diet inclusion level. Comparison was made to a control containing conventionally produced soybean meal. Performance (body weight, feed intake and feed efficiency) was collected through to time of marketing.The European Efficiency Index (EEI) Score was used to assess the combined effect of rate of gain and feed conversion ratio. Diets containing 2.5% Nutrivance or 2.5% Fermotide or Neptide had an EEI average score of 5 while the control score was 4.6. It was concluded when feeding to 6 wks of age, Nutrivance at 2.5% resulted in the best turkey tom performance to market weight followed by Fermotide and Neptide products in comparison to the control. An acceptable inclusion level in turkey diets was 2.5% and recommended to be fed to six weeks of age.

Publications

  • Type: Other Status: Published Year Published: 2020 Citation: Noll, S. L. Enhancing poultry performance with specialized soy products. https://www.auri.org/2020/01/enhancing-poultry-performance-with-specialized-soy-products/


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:The target audience varies depending on topic. For nutrition the audience includes turkey producers that produce their own turkey feed; commercial poultry nutritionists and consultants; poultry feed industry; and amino acid suppliers. For biosecurity, the audience is poultry producers, extension youth specialists, veterinarians, and 4-H youth. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Undergraduates (5) received training in data handling, analyses and data sumarization. One veterinary student received training in abstract writing, preparing a scientific presentation, and presenting at a national meeting. How have the results been disseminated to communities of interest?The results of the amino acid and soybean meal studies were reported to turkey producers and commercial poultry nutritionists at a regional nutrition conference (Minnesota Nutrition Conference). For the biosecurity work, these were presented at a national/international poultry science meeting in San Antonio, Texas. What do you plan to do during the next reporting period to accomplish the goals?Upcoming work with emphasize the following goals: 1. Determine the dietary lysine requirement for market turkeys at different ages 4. Determine efficacy of biosecurity implementation and litter management to decrease disease transmission and footpad incidence and severity 5. Determine performance of turkeys fed diets containing antibiotic alternatives, high quality/alternative ingredients or with varying diet nutrient density. 6. Within studies for the objectives above, assess development of the gut, microbial community and health status of birds.

Impacts
What was accomplished under these goals? For Goal #2, the amino acid nutrition of turkeys was examined in the 9 to 12 wk age period for commercial market toms. The following ratios of amino acids to lysine on a digestible amino acid basis wer found to be adequate for growth and feed efficiency: methionine (0.38), threonine (.62), arginine (1.03), and valine (.74). For Goal #4, biosecurity practices at fairs was examined by surveying 4-H participants at the time they checked in their birds. Based on survey results, there is a need for on-going education regarding biosecurity in fair poultry. In particular, aspects of quarantine and combining other standard biosecurity practices are needed to improve risk before, during, and after fair activities. For Goal #5, the use of a highly digestible type soybean meal was examined for use in commercial market toms. The soybean meal was processed to reduce the content of complex carbohydrates (oligosaccharides) which are not digested by poultry. The true metabolizable energy content of the specially processed meal was increased over that of conventionally produced soybean meal by 9.4%.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Noll, SL. 2018. Nutritive value of modified soybean meal for meat-type poultry. In Proceedings 79th Minnesota Nutrition Conference, Department of Animal Science, University of Minnesota and University of Minnesota Extension. p 25-28.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Noll, SL. 2018. University of Minnesota Research Update: Amino Acids for Commercial Turkey Toms. In Proceedings 79th Minnesota Nutrition Conference, Department of Animal Science, University of Minnesota and University of Minnesota Extension. p. 183.
  • Type: Other Status: Published Year Published: 2018 Citation: Olson, M. H. Martin, C. Cardona, and S. Noll, 2018. Assessing educational needs and practices of poultry fair participants relative to biosecurity and avian influenza. Poultry Sci. (E-Supplement 1) 97:41


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:The target audience includes turkey producers that produce their own turkey feed; commercial poultry nutritionists and consultants; poutlry feed industry; and amino acid suppliers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A graduate student was trained in the development of regression models for the determination of the lysine requirement. Undergraduate students were trained in data handling, analyses and data summarization. How have the results been disseminated to communities of interest?The results of the amino acid studies were reported to turkey producers and commercial poultry nutritionists at a national meeting - "Turkey Amino Acid Nutrition Summit" - held November in Minneapolis MN. What do you plan to do during the next reporting period to accomplish the goals? Complete the lysine requirement determination in 9 to 12 wk old turkeys Determine turkey response sensitivity to ratios of digestible arginine, methionine, threonine and valine relative to lysine in 9 to 12 wk old turkeys

Impacts
What was accomplished under these goals? Research emphasis was focused on Goal #1 (Determine the dietary lysine requirement for market turkeys at different ages) and Goal #2 (Determine the sensitivity in diet amino acid ratios for market turkeys at different ages). For Goal #1, the digestible lysine requirement was determined for 16 to 19 wk old male market turkeys. An estimated 1.07% digestible lysine requirement was determined based on turkey gain. The requirement was determined using male Hybrid Converter turkeys. Diets with different levels of dlys were fed to 10 replicate pens (10 turkeys/pen). The digestible lysine requirement for gain was substantially greater than the estimated dlys requirement previously published by the NRC (1.07% vs .7% dlys) confirming the need for updated requirement information. For Goal #2, diets with different ratios (low, medium, high) of the digestible amino acids relative to dietary lysine were fed to male market turkeys during 0 to 4 wks and 16 to 19 wks of age. During 0 to 4 wk of age for the amino acids arginine, methionine plus cystine, threonine, and valine, the following respective ratios were found to be acceptable: 1.06, .65, .60, and .75. During 16 to 19 wk of age for arginine, methionine plus cystine, threonine, and valine, the following respective ratios were found to be acceptable: 1.01, .76, .64, and .75. These studies provide greatly needed information on amino acid ratios for turkeys. Diets formulated with appropriate amino acid ratios will lessen the excretion of unused amino acids in the turkey manure.

Publications