Progress 09/01/16 to 08/31/21
Outputs
Target Audience:Target audiences include US university graduate schools, Oklahoma State University, Agricultural Biosecurity communites, APHIS, CPHIST, FBI, and State agricultural agencies Changes/Problems:There was a delay in recruiting the last student and so this student was brought onto the project during the last year. He will complete his requirements for graduation after the project has ended. What opportunities for training and professional development has the project provided?Three of the four students who have completed their degrees participated in the Ag Biosecurity Short Course held at Kansas State University during different years. They also participated in educational opportunities for 4-H students in two field days which featured mock plant pathology emergencies and investigations that were hosted by NIMFFAB. These students also participated in a mock investigation and trial of persons accused of carrying out a domestic agricultural attack. These students all completed their internships which led to greater opportunities after graduate school. One student is now employed by the FBI at Quantico, one student is now employed at NASA and the last student is employed at a major plant disease diagnostic laboratory. The fourth student is still completing his degree. How have the results been disseminated to communities of interest?All students presented at professional meetings or published research results. These are listed below: P. Rydzak. EDNA-Wheat, Using NGS as a Tool to Detect Viruses in Wheat Metagenomic Data. 2018. American Phytopathological Society, Boston, MA. Conference Papers and Presentations2018 S. Wallace. Sara Wallace, J. Olson, FM Ochoa-Corona, A. Harris. Investigating the cause of red blotch disease in Grapevines (Vitis spp) in Oklahoma. (Abstr.) Phytopathology 108: S1.66 https://doi.org/10.1094/PHYTO-108-10-S1.1 Theses/Dissertations2018 Wallace, S. Sara Wallace, J. Olson, FM Ochoa-Corona, A. Harris. Investigating the cause of red blotch disease in Grapevines (Vitis spp) in Oklahoma. (Abstr.) Phytopathology 108: S1.66 https://doi.org/10.1094/PHYTO-108-10-S1.1 Other2018 Wallace, S. DETECTION AND DIAGNOSIS OF RED LEAF DISEASES OF GRAPES (VITIS SPP) IN OKLAHOMA, 2018. Master's thesis, Oklahoma State University. Theses/Dissertations2018 Rydzak, Patrick. 2019. DETECTION OF ARTHROPOD TRANSMITTED VIRUSES OF CEREAL CROPS AND RNAi INDUCED RESISTANCE TO WHEAT STREAK MOSAIC VIRUS. PhD Dissertation. Oklahoma State University, Stillwater, OK 74078 Theses/Dissertations2019 Sharp, Marie. 2019. Metagenomic analysis of the microbial community on fresh tomatoes. PhD Dissertation. Oklahoma State University, Stillwater, OK 74078. Theses/Dissertations2019 Wallace, Sara. 2019. Detection of the pathogen that causes red blotch disease in grapevine. Master's Thesis. Oklahoma State University, Stillwater, OK 74078 Theses/Dissertations2019 Rydzak, P. Detection of arthropod-transmitted viruses in cereal crops and RNAi induced resistance to Wheat streak mosaic virus. PhD defense seminar, April 2019, Oklahoma State University, Stillwater, OK. Conference Papers and Presentations2019 Rydzak, P., F. Ochoa-Corona, A. Whitfield, and A. Wayadande. 2020. Combining multiplex PCR and high-resolution melting for the detection and discrimination of arthropod transmitted viruses of cereals. J. Virological Meth. 278: 113823. Journal Articles2020 What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
1. Four highly qualified graduate students were recruited: two master's students and two doctoral students 2. Three of the four students completed their master's or doctorates in Plant Pathology. The remaining student is on track for graduation in food safety within Animal Science. 3. Three of the four students completed their internships. Twodoctoral students interned at the FBI Quantico Laboratoryand one master's student interned at APHIS PPQ in Raleigh, NC. The remaining student has not yet completed his internship. 4. Three of the four students have completed their degrees and are now employed. The remaining student is on track for graduation. They all participated in NIMFFAB activities and were leaders within the graduate group of students in Plant Pathology at Oklahoma State University. 5. Research results were disseminated to professional communities such as scientific societies, seminars, and student exchanges. One PI attended the NACTA meeting in 2016to report progress.
Publications
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Progress 09/01/19 to 08/31/20
Outputs
Target Audience:Academic research communities and governmental regulatory officials. Changes/Problems:Further funding for this graduate student will be covered by other sources upon the completion of this project (Aug 2021). What opportunities for training and professional development has the project provided?The graduate student has begun his coursework and is learning laboratory methods and experimental design in food microbiology which can then be applied to microbial forensics. To date, the student has received training in BSL-2 laboratory procedures and the safe handling of food-borne pathogens. How have the results been disseminated to communities of interest?The one publication from a former NNF student was published in a peer-reviewed journal. What do you plan to do during the next reporting period to accomplish the goals?During the next year, the last graduate student in this cohort should complete his major course work, accomplish part of his research objectives, and complete his internship.
Impacts
What was accomplished under these goals?
This is the fourth year of the project and the PIs were able to identify one final graduate student. This person began his master's program in the Fall of 2020. He began taking graduate courses and started his research project under the direction of Dr. Li Ma. Thus, one objective of identifying four qualified graduate students has been met.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Rydzak, P., F. Ochoa-Corona, A. Whitfield, and A. Wayadande. 2020. Combining multiplex PCR and high-resolution melting for the detection and discrimination of arthropod transmitted viruses of cereals. J. Virological Meth. 278: 113823.
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Progress 09/01/18 to 08/31/19
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Each student learned skills that enabled each of them to enter the work force in their area of interest. Sara Wallace worked in the plant diagnostic lab at Oklahoma State University and used PCR-based tools to sample and characterize Red blotch disease incidence in Oklahoma. Marie Sharp learned bioinformatics tools, obtained a separate certificate in bioinformatics, and conducted research with genomic and transcriptomic datasets. Patrick Rydzak developed advanced diagnostic tools for wheat infecting viruses. Each student attended professional conferences and presented their research. Impact: Each student graduated on time and went on to a professional career at a State or National security facility. Sara Wallace was hired by Oklahoma Department of Agriculture, Food and Forestry. Marie Sharp was hired by the National Aeronautics and Space Administration and Patrick Rydzak was hired by the Federal Bureau of Investigation Research Laboratory at Quantico, VA. How have the results been disseminated to communities of interest?The results were the successful graduation of the three students. Their theses and dissertations are housed at the OSU Library. The research that resulted from their work as they completed their degree requirements was presented at professional conferences in late 2018-early 2019. What do you plan to do during the next reporting period to accomplish the goals?In the last year of the project, the results from the student research will be submitted for publication.
Impacts
What was accomplished under these goals?
Three graduate students (one master's and two doctoral students) were recruited in 2016 and 2017 to Oklahoma State University to pursue degrees related to agricultural biosecurity. The overall goals of the program were to recruit and retain students to complete degrees in Plant Pathology that would enable them to pursue further education or jobs in law enforcement or biosecurity. During the summer of 2018 and fall of 2018 the three students (Sara Wallace, Marie Sharp, and Patrick Rydzak) completed their internships at three federal agencies. Ms. Wallace completed a four week internship at USDA APHIS CPHIST in Raleigh N.C. Patrick Rydzak and Marie Sharp both completed 5 and 6 week internships at the FBI Research Laboratory at Quantico, VA. During 2018 and 2019, each student completed research that culminated in each of them receiving their graduate degree in Plant Pathology from Oklahoma State University.
Publications
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2019
Citation:
Rydzak, Patrick. 2019. DETECTION OF ARTHROPOD TRANSMITTED VIRUSES
OF CEREAL CROPS AND RNAi INDUCED RESISTANCE TO WHEAT STREAK MOSAIC VIRUS. PhD Dissertation. Oklahoma State University, Stillwater, OK 74078
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2019
Citation:
Sharp, Marie. 2019. Metagenomic analysis of the microbial community on fresh tomatoes. PhD Dissertation. Oklahoma State University, Stillwater, OK 74078.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2019
Citation:
Wallace, Sara. 2019. Detection of the pathogen that causes red blotch disease in grapevine. Masters Thesis. Oklahoma State University, Stillwater, OK 74078
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2019
Citation:
Rydzak, P. Detection of arthropod-transmitted viruses in cereal crops and RNAi induced resistance to Wheat streak mosaic virus. Ph.D defense seminar, April, 2019, Oklahoma State University, Stillwater, OK.
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Progress 09/01/17 to 08/31/18
Outputs
Target Audience:Academia, USDA, FBI, other law enforcement agencies. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The master's student had the opportunity to work directly at the university plant disease diagnostic laboratory and this gave her the experience to successfully apply for a position at the Oklahoma Department of Agriculture, Food and Forestry (where she currently works). How have the results been disseminated to communities of interest?All students have attended professional meetings (American Phytopathological Society) and presented their research results. What do you plan to do during the next reporting period to accomplish the goals?In the next year we expect the final two students to complete their studies and graduate. Each student will submit manuscripts to peer-reviewed journals for publication.
Impacts
What was accomplished under these goals?
All of the students have completed their internships. Two students went to the FBI center in Quantico, VA for 6 and 8-week internships respectively, and the other student went to USDA-PERAL in Raleigh, NC.One master's student completed research leading to her degree. The two doctoral students are continuing their research with plans to graduate in 2019.
Publications
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2018
Citation:
S. Wallace. Sara Wallace, J. Olson, FM Ochoa-Corona, A. Harris. Investigating the cause of red blotch disease in Grapevines (Vitis spp) in Oklahoma. (Abstr.) Phytopathology 108: S1.66 https://doi.org/10.1094/PHYTO-108-10-S1.1
- Type:
Other
Status:
Published
Year Published:
2018
Citation:
Wallace, S. Sara Wallace, J. Olson, FM Ochoa-Corona, A. Harris. Investigating the cause of red blotch disease in Grapevines (Vitis spp) in Oklahoma. (Abstr.) Phytopathology 108: S1.66 https://doi.org/10.1094/PHYTO-108-10-S1.1
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2018
Citation:
Wallace, S. DETECTION AND DIAGNOSIS OF RED LEAF DISEASES OF GRAPES (VITIS SPP) IN OKLAHOMA, 2018. Master⿿s thesis, Oklahoma State University.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
P. Rydzak. EDNA-Wheat, Using NGS as a Tool to Detect Viruses in Wheat Metagenomic Data. 2018. American Phytopathological Society, Boston, MA.
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Progress 09/01/16 to 08/31/17
Outputs
Target Audience:The agricultural biosecurity mission of the National Institute for Microbial Forensics & Food and Agricultural Biosecurity at Oklahoma State University is served, in part, by educating graduate students in preparation for professional positions in government agencies, law enforcement, or academia. To that end, we have used the National Needs Fellowships as an opportunity to provide high quality graduate education in plant pathogen forensics at Oklahoma State. Our target audience is broad; we want to reachagricultural biosecurity agencies, such as USDA APHIS andCPHIST, law enforcement such as FBI,regulatory agencies such as FDA and CDC and other research institutions such at the National Laboratories, as well as any other pertinent body that is interested in biosecurity. In addition to the above mentioned institutions, we also want to reach out to other universities with an agricultural biosecurity emphasis and agencies such as Department of Defense and Homeland Security. Changes/Problems:We have had some difficulty recruiting the final student (MS), but are in a major recruiting effort for a student that will begin during Fall 2018. What opportunities for training and professional development has the project provided?At NIMFFAB, opportunities for professional development occur within the individual's research project and also with group/community activities. One doctoral student attended a Gordon Conference and made several professional contacts. Last year, all three students attended an Agricultural Biosecurity Conference at Kansas State University, and availed themselves of the opportunity to meet and learn from Ag Biosecurity experts from around the United States and other countries. The students participate in weekly NIMFFAB seminar which highlights a nationally known or local scientist involved in biosecurity or microbial forensics research. Finally, all of the students are actively engaged in the Department of Entomology and Plant Pathology, serving in the graduate student association (EPPGSA) and in their professional societies. How have the results been disseminated to communities of interest?For this cohort of students, research results have been presented at professional societies and locallly within the Department of Entomology and Plant Pathology. This last year, one doctoral student presented a paper ata Gordon Conference, and another student presented a poster atthe American Phytoplathological Society national meeting. There have also been opportunities to communicate with law enforcement through visits to NIMFFAB. During these visits, the three NNF students are encouraged to interact with the FBI or APHIS speakers. What do you plan to do during the next reporting period to accomplish the goals?During the next period, the three students will complete their TESA-related internships (2 at FBI, 1 at a local national importer of plants). The students will also be completing their cybersymposium and reporting results of their research at national meetings.
Impacts
What was accomplished under these goals?
In the first year, we recruited three excellent graduate students; two doctoral students and one master's student. We are still actively recruiting the final master's student for the NNF program at NIMFFAB. Each of the current students has submitted a plan of study and are currently on-track to complete required coursework. All three students have initiated thier research projects; one is investigating bioinformatics of food borne pathogens in fresh produce, another is involved in diagnostics (both wet-lab and in silico) of plant pathogenic viruses, and the last student is involved in developing diagnostic tools for an important gravevine virus. All three students are on-track to participate in their TESA-related internships during the summer of 2018. Finally, the two doctoral students have attended national professional meetings and presented their research results. All three students are very active locally and have participated in Oklahoma State paper and poster competitions.
Publications
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