Progress 09/01/16 to 08/31/21
Outputs
Target Audience:Target audiences reached this past year included large and small-scale agricultural producers, homeowners, plant production and nursery operators, diagnosticians, state (Departments of Agriculture) and federal (USDA APHIS Plant Protection and Quarantine) plant regulatory personnel, and plant industry (nursery, seed) support professionals. The Great Plains Diagnostic Network (GPDN) is one of five regional networks of the National Plant Diagnsotic Network and is comprised of representatives of Land Grant Universities and Agricultural Experiment Stations from the following eight states; Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, and Wyoming. GPDN is comprised of a team of specialists skilled at rapid and accurate identification of plant pests and pathogens, timely communications of diagnostic test results, and the delivery of training programs to facilitate early detection and mitigation of pest and disease outbreaks that impact large and small-scale agricultural production systems, commercial horticultural production and retail systems, as well as homeowner environments. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Professional Development: Provide training opportunities and experiential learning that accelerate the learning curve and enhance diagnostic capabilities of current and future diagnosticians. GPDN provided leadership for the newly established NPDN Professional Development Program. The NPDN National Professional Development Coordinator is a member of GPDN and led the licensing of the industry-leading learning management system to deliver high quality and timely education and training content to the entire network. PCR educational modules were designed and developed as part of a diagnostics curriculum to provide the foundation for NPDN labs to implement molecular-based technologies in their labs. GPDN convened two national workshops based on recent introductions of two high consequence regulated plant pathogens. The scenario-based workshops included representatives from the state and federal regulatory agencies that were involved in the actual events as well as the NPDN diagnosticians who processed the samples and made the original diagnoses. The workshops highlighted proper handling and tracking of samples to maintain security and integrity of the samples as well as secure and timely communications when regulatory samples are received. One workshop focused on the Phytophthora ramorum detection in Indiana that originated in Oklahoma while the second workshop focused on the detection of the Select Agent Ralstonia solanacearum r3b2 in Michigan. Both workshops were well attended with over 75 participants from all five NPDN regions in each workshop. GPDN diagnosticians participated in the APHIS PPQ Beltsville Workshops which were virtual this year due to COVID restrictions in government facilities. GPDN diagnosticians and other personnel participated in 31 NPDN professional development opportunities, attended 19 professional conferences, and participated in 17 miscellaneous training events. The GPDN Regional Webinar Series with national coverage continued this past project year providing all NPDN states with the most recent information on several emerging and remerging pathogens and insect pests. The GPDN webinar series has been successfully delivered for 15 years. First Detector training sessions for Master Gardeners were conducted in several GPDN states. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state estimated to have reaching thousands of people. GPDN members conducted many training sessions for First Detectors delivering 309 presentations relevant to the NPDN mission through a variety of media platforms (in-person, virtual, social media, etc.) reaching just under 200,000 participants. Many First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak but, were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Three bed bug workshops were conducted where 82% of participants increased their knowledge about detecting bed bug infestations, 86% increased their knowledge about bed bug biology, and 73% were likely to make monitoring/inspection changes for bed bugs based of the information learned at the workshops. Nebraska taught graduate and undergraduate students to look at and diagnose plant health problems. The graduate students gained a better understanding of economically important plant pathogens and the ability to diagnose plants in the field. Chandler Day (Kansas) served as the GPDN regional Training and Education Coordinator. State training coordinators are Ana Cristina Fulladolsa (CO), Jim Stack & Judy O'Mara (KS), Mary Burrows (MT), Kyle Broderick (NE), Jesse Ostrander (ND), Jen Olson (OK), Emmanuel Byamukama (SD), and William Stump (WY). How have the results been disseminated to communities of interest?GPDN members conducted many training sessions for First Detectors delivering at least 309 presentations relevant to the NPDN mission through a variety of media platforms including, social media, in-person, virtual, and reaching just under 200,000 participants. Many First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak but were not organized by GPDN members; consequently, registration of attendees did not occur at those events. First Detector training sessions for Master Gardeners were conducted in several GPDN states. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state estimated to have reached over 17,028 people. Oklahoma produced several plant disease and pest awareness programs that were either widely broadcast over radio or televised throughout the state estimated to have reached over 176,883 people. The GPDN Regional Webinar Series with national coverage continued this past project year providing all NPDN states with the most recent information on several emerging and remerging pathogens and insect pests. Coordinated by Montana State University, the GPDN webinar series has been successfully delivered for 15 years. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Quality diagnostics: Provide high quality diagnostics to support plant health communities through constant improvement and quality assurance. With 22 diagnosticians (23.95 FTE) in eight states, GPDN had a very productive year having recorded 10 first detections in the region, uploaded 13,959 samples (21,589 diagnoses) to the NPDN National Data Repository and 4,200 entries to non-NPDN databases, while addressing the range of plant health issues including, abiotic (1,179 samples), arthropod (1,798 samples), nematode (1,376 samples), pathogen (15,566 samples), and plant/weed (233 samples). COLORADO recorded a first report of High plains wheat mosaic virus in corn, (Zea mays L.). MONTANA recorded three first detections for the state: a first report of Thielaviopsis spp. in ln Lupine and field pea, a first report of Verticillium spp. on chickpea, as well as, a first report of the old house borer, Hylotrupes bajulus (Coleoptera: Cerambycidae). They helped confirm three new county reports of plants problematic for Montana agriculture [waterhemp (Amaranthus tuburculatus), Roosevelt County; Italian ryegrass (Lolium persicum), Judith Basin County; and common barberry (Berberis vulgaris), Fergus County]. NEBRASKA recorded a first report of Trypanosoma cruzi of significance to human health. OKLAHOMA played a significant role in identifying the detection of Tomato Brown Rugose Fruit Virus in Nebraska as well as, assisting the Oklahoma Department of Agriculture in the new county detections (Noble and Custer) of the Red Imported Fire Ant. SOUTH DAKOTA recorded a first report of Phytophthora sansomeana. The GPDN Regional Assistant Director, Chanlder Day, successullly executed the NPPLAP Certification Proficiency Panel for Phytopthora ramorum thus allowing the GPDN Regional Lab to become the 3rd NPDN regional center lab certified to support P. ramorum trace forward and trace back detection events. GPDN diagnosticians continued to expand the capabilities of their labs through increased adoption of more technologies including, isothermal amplification of nucleic acids, e.g., RPA (recombinase-polymerase amplification) with the AmpliFire device. The COLORADO Plant Diagnostic Clinic (PDC) provided regulatory testing services for multiple crops, submitted by growers, consultants, or the Colorado Department of Agriculture. They continued the implementation of multiple optimized protocols for industrial hemp pathogen testing including, detection protocols for Pseudomonas cannabina and Xanthomonas cannabis. They also implemented detection protocols for other hemp pathogens including, alfalfa mosaic virus, arabis mosaic virus, cucumber mosaic viruses, tobamoviruses (via TMV ELISA), hop stunt viroid, Fusarium species-specific ID via multi-locus sequence analysis, Phytophthora infestans, Dickeya chrysanthemi, and Sclerotinia sclerotiorum. These detection tests contributed to supporting trade of industrial hemp with other countries. Additionally, the implementation of these tests has increased the lab's capabilities, our knowledge of potential pathogen threats and disease concerns, reference materials/controls for pathogen testing, and demand for services. It has also increased our communication and collaboration with state regulatory officials. KANSAS partnered with the Kansas Department of Agriculture providing diagnostic services for 114 water and plant samples to support a CAPS-sponsored Phytophthora ramorum survey: 41% tested positive for Phytophthora species and no P. ramorum was detected. The Kansas State Diagnostic lab remained open throughout the COVID-19 pandemic providing diagnostic services to a range of clientele. Following Montana State University policies, the Schutter Plant Diagnostic lab (SDL) was able to operate and offer diagnostic services to stakeholders throughout the COVID pandemic. The SDL encouraged clients to submit samples digitally and would ask for a physical sample if needed. Clients could/can email us photos or try our sample submission app. The SDL is open for walk-in clients from 9:00 to 12:00 Monday - Friday. Clients are asked to wear a mask, keep a 6-foot distance from staff and other clients, and wash hands before and after visiting the lab. The OKLAHOMA State University Plant and Insect Diagnostic Lab completed a major renovation significantly increasing their BSL-2 space that now houses 2 biosafety cabinets, commercial refrigerators within the lab to ensure sample security and integrity, PCR clean-benches and a real-time PCR system. SOUTH DAKOTA also updated equipment by acquiring a new autoclave and incubator. WYOMING logged their highest number of samples in the past 6 years. GPDN continued promotion of the NPDN laboratory accreditation program across the GPDN region. The NPDN lab accreditation program is designed to implement and sustain high laboratory management standards that underpin quality diagnostics. GPDN member labs were kept informed about changes to the STAR-D accreditation program as well as to training opportunities. The GPDN Director contributed to the evaluation of the laboratory accreditation program and the development of the new CORE standard to be implemented in all NPDN labs during the next cooperative agreement. The GPDN Regional Center Lab as well as some GPDN state diagnostic labs provided diagnostic support to state and federal (USDA APHIS PPQ) agencies during two national plant health emergencies, Phytophthora ramorum blight detection in Indiana and Oklahoma and the Ralstonia solanacearum detgection in Michigan.
Publications
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Nachappa, P., Fulladolsa, A.C., Stenglein, M. 2020. Wild Wild West: emerging viruses and viroids of hemp. Outlooks on Pest Management 31(4):175-179. DOI: https://doi.org/10.1564/v31_aug_07
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Reynoso, Matias. 2020. Soft rot and blackleg of potato. CSU Plant Diagnostic Clinic Website: Featured Disease. https://plantclinic.agsci.colostate.edu/#1589584033302-877c914a-1ca6
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Janet Hardin. 2020. Hoary Comma. CSU Plant Diagnostic Clinic Website: Featured Arthropod. https://plantclinic.agsci.colostate.edu/#1589584033302-877c914a-1ca6
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Giebler, M. and Nelson, A. 2020. Pinewood nematode. CSU Plant Diagnostic Clinic Website: Featured Disease. https://plantclinic.agsci.colostate.edu/#1589584033302-877c914a-1ca6
- Type:
Other
Status:
Published
Year Published:
2019
Citation:
OMara J. Report from the K-State Plant Pathology Diagnostic Lab. 2019 Kansas Forest Health Highlights. Released in 2020.1. Numbered state extension publications
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Andersen Onofre, K. and De Wolf, E. D. 2020. Foliar fungicide efficacy ratings for wheat disease management 2020. Kansas State University Agricultural Experiment Station and Cooperative Extension Service. Pub No. EP-130
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Andersen Onofre, K., De Wolf, E. D., Lollato, R. and Whitworth, J. R. 2020. Wheat Variety Disease and Insect Ratings 2020. Kansas State University Agricultural Experiment Station and Cooperative Extension Service. Pub No. MF991.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Andersen Onofre, K., De Wolf, E. D. Seed treatment fungicides for wheat disease management 2020. Kansas State University Agricultural Experiment Station and Cooperative Extension Service. Pub No. MF2955.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Moparthi, S., K. McPhee, B. Agindotan, C. Peluola, and M. Burrows. 2020. First report of gray mold of chickpea caused by Botrytis euroamericana. Crop Protection, Volume 137, https://doi.org/10.1016/j.cropro.2020.105297
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Moparthi, S., M. Burrows, J. Mgbechi-Ezeri, and B. Agindotan. Fusarium spp. associated with root rot of pulse crops and their cross pathogenicity to cereal crops in Montana. Plant Disease, doi: 10.1094/PDIS-04-20-0800-RE
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Parikh, L., B. Agindotan, and M. Burrows. 2020. Antifungal activity of essential oils on pathogens of pulse crops. Plant Disease, https://doi.org/10.1094/PDIS-06-20-1401-RE
- Type:
Book Chapters
Status:
Published
Year Published:
2020
Citation:
Harveson, R., W. Chen, M. Burrows, J. Pasche and L. Porter. 2020. Compendium of Pea Diseases and Pests. APS Press
- Type:
Book Chapters
Status:
Published
Year Published:
2020
Citation:
Smith, D., K. Wise, A. Freije, A. Sisson, A. Friskop, A. Tenuta, E. Byamukama, J. Marshall, M. Burrows, D. Mueller. 2020. A Farmers Guide to Wheat Diseases. APS Press, St. Paul
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Moore-Gough, C., T. Dougher, E. Grimme, L. Kerzicnik, N. Orloff. 2020. Maintaining successful lawns. Montana State University Extension MontGuide. MT202004. 4 pp.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Moore-Gough, C., E. Grimme, L. Kerzicnik, D. Palmer, P. Flaherty. 2020. Successful home vegetable gardening. Montana State University Extension MontGuide. MT202002. 12 pp.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Grimme, E., Miller, Z., Moore-Gough, C., Zidack, N., and M. Burrows. MSU Extension - MontGuide: Important Apple Diseases in Montana and Recommended Varieties for Resistance. January 2020. MT200812. 8 pp
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Orloff, L. N., Mangold, J., Seipel, T. F. (2020). A Guide to Diagnosing Non-Target Herbicide Injury on Plants (EB0232 ed., pp. 38). Bozeman, Montana: Montana State University Extension.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Seipel, T. F., Mangold, J., Fine, T., Pokorny, M., Orloff, L. N. (2020). Palmer amaranth (Amaranthus palmeri) (MT202011AG ed., pp. 4). Bozeman, MT: Montana State University Extension.
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K. (2020). September Plant and Pest Diagnostic Clinic Disease Update. https://cropwatch.unl.edu/2020/september-2020-disease-reports-unl-diagnostic-clinic
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K. (2020). August Plant and Pest Diagnostic Clinic Disease Update. https://cropwatch.unl.edu/2020/september-2020-disease-reports-unl-diagnostic-clinic
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Mangold, J., Orloff, L. N. et. al. 2020. Plants Poisonous to Livestock in Montana and Wyoming (Plant Materials Technical Note MT-124 ed., pp. 43). Bozeman, Montana: USDA-NRCS, Bozeman State Office
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Kerzicnik, L. et al. 2020. Emerald ash borer. Montana State University Extension and others. 4 pp
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
McKelvy, U., Rupp, J., Zidack, N., and Jacobsen, B. 2020. 2020 Montana Seed Potato Disease Management Guide. Montana State University Extension. Available online: http://www.montanaspud.org/resources_and_guides.html
- Type:
Other
Status:
Awaiting Publication
Year Published:
2021
Citation:
Streich, A., Muhle, A., Broderick, K., Giesler, L., and Green, J. (In Press). Integrated Pest Management: Turfgrass Diseases and Insects. Integrated Turfgrass Management of the Great Plains
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Jackson-Ziems, T., Broderick, K. (2020). While You’re Waiting, Collect a Sample for SCN Analysis. https://cropwatch.unl.edu/2020/while-youre-waiting-collect-sample-scn-analysis
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Jackson-Ziems, T., Broderick, K. (2020). While Youre Waiting, Collect a Sample for SCN Analysis Norfolk Daily News; September 24, 2020.
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K. (2020). Late July Plant and Pest Diagnostic Clinic Disease Update. https://cropwatch.unl.edu/2020/disease-reports-unl-diagnostic-clinic-july-31
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K. (2020). Early July Plant and Pest Diagnostic Clinic Disease Update. https://cropwatch.unl.edu/2020/july-2020-disease-reports-unl-diagnostic-clinic
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K. (2020). Plant and Pest Diagnostic Clinic; Updated Submission Procedures. https://cropwatch.unl.edu/2020/plant-and-pest-diagnostic-clinic-updated-submission-procedures
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K. (2020). Plant & Pest Diagnostic Clinic Adjusts to COVID-19 Disruption. https://cropwatch.unl.edu/2020/plant-pest-diagnostic-clinic-adjusts-covid-19-disruption
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Broderick, K and Jackson-Ziems, T. 2020. Soybean Disease Update. Proceedings of the Nebraska Crop Management Conference. Nebraska Extension. https://cropwatch.unl.edu/2020/soybean-disease-update
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., K. Koch, J. McMechan. April 23, 2020. Cutworm Pheromone Trapping Network Update. CropWatch Web Article. Producers in Eastern NE. https://cropwatch.unl.edu/2020/pheromone-trapping-network-starting-show-low-levels-cutworm-moths
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., K. Koch, J. McMechan. May 1, 2020. Cutworm Pheromone Trapping Network Update. CropWatch Web Article. Producers in Eastern NE. https://cropwatch.unl.edu/2020/pheromone-trapping-network-starting-show-low-levels-cutworm-moths
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., K. Koch, J. McMechan. May 8, 2020. Cutworm Pheromone Trapping Network Update. CropWatch Web Article. Producers in Eastern NE. https://cropwatch.unl.edu/2020/pheromone-trapping-network-starting-show-low-levels-cutworm-moths
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., K. Koch, J. McMechan. May 13, 2020. Scout Emerging Corn for Insects; Don’t Assume Protection. CropWatch Web Article. Corn Producers in NE. https://cropwatch.unl.edu/2020/scout-emerging-corn-insects-dont-assume-protection
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., K. Koch, J. McMechan. May 15, 2020. Cutworm Pheromone Trapping Network Update. CropWatch Web Article. Producers in Eastern NE. https://cropwatch.unl.edu/2020/pheromone-trapping-network-starting-show-low-levels-cutworm-moths
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., K. Koch, J. McMechan. May 22, 2020. Cutworm Pheromone Trapping Network Update. CropWatch Web Article. Producers in Eastern NE. https://cropwatch.unl.edu/2020/pheromone-trapping-network-starting-show-low-levels-cutworm-moths
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Wright, R., J. McMechan, K. Koch. June 23, 2020. Japanese Beetles Emerging; Scout Corn and Soybean Fields. CropWatch Web Article. Corn and soybean producers in NE. https://cropwatch.unl.edu/2019/japanese-beetles
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Tande, C., Dorrance, E. A., Schwarzrock, D., Mahecha, E., and Byamukama, E. 2020. First report of Phytophthora sansomeana causing root rot of soybean in South Dakota. Plant Disease 104:1877
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Byamukama, E., Tande, C., Nampijja, M., Mathew, F., and Bleakley, B. 2020. First report of Xanthomonas vasicola pv. vasculorum, the causal agent of bacterial leaf streak of corn, in South Dakota. Plant Disease 106: 1851
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Kerzicnik, L. Ash Pests on the Rise. AMTOPP Quarterly Newsletter. 1 pp. Fall 2020
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Kerzicnik, L. Red-Naped Sapsuckers. AMTOPP Quarterly Newsletter. 1 pp. Summer 2020.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Kerzicnik, L. Don’t Always Fear when Wasps are Near. AMTOPP Quarterly Newsletter. 1 pp. Spring 2020
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Kerzicnik, L. Uninvited Fall Guests. Lives and Landscapes. Extension Publication. 2 pp. Sep 2020.
Giant woodwasps. Montana Ag Live Newsletter. 1 pp. 31 Aug 2020
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Organic management options for grasshoppers. Montana Ag Live Newsletter. 1 pp. 26 Aug 2020
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Orloff, N. 2020. Managing Canada thistle in lawns. Monthly Weed Post. Montana State University Extension. Aug. 2020
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Kerzicnik, L. Dont always fear when wasps are near. 3/20/20. AMTOPP Quarterly Newsletter. Arborists, landscape, and pest professionals.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Orloff, N. Hairy cats ear (Hypochaeris radicata). January, 2020. MSU Extension Monthly Weed Post.
- Type:
Other
Status:
Published
Year Published:
2020
Citation:
Orloff, N. 2020. Non-target herbicide injury on plants. Monthly Weed Post. Montana State University Extension. Nov. 2020
|
Progress 09/01/19 to 08/31/20
Outputs
Target Audience:Target Audience Target audiences reached this past year included large and small-scale agricultural producers, homeowners, plant production and nursery operators, diagnosticians, state (Departments of Agriculture) and federal (USDA APHIS Plant Protection and Quarantine) plant regulatory personnel, and plant industry (nursery, seed) support professionals. The Great Plains Diagnostic Network (GPDN) is one of five regional networks of the National Plant Diagnsotic Network and is comprised of representatives of Land Grant Universities and Agricultural Experiment Stations from the following nine states; Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming. GPDN is comprised of a team of specialists skilled at rapid and accurate identification of plant pests and pathogens, timely communications of diagnostic test results, and the delivery of training programs to facilitate early detection and mitigation of pest and disease outbreaks that impact large and small-scale agrilcutultural production systems, commercial horticultural production and retail systems, as well as homeowner environments. Changes/Problems:Beginning in the next project year, Texas will no longer be a member state of GPDN. Texas will be represent3ed a one state within NPDN and be administered through SPDN. What opportunities for training and professional development has the project provided? Total number of training sessions and total number of First Detectors trained: GPDN members conducted many training sessions for First Detectors. The number of sessions conducted and the number of First Detectors trained are included in the total numbers presented in Table 1. At least 3,109 First Detectors received some first detector training at with least 60 presentations/publications. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Summary of regional training efforts: Christian Webb (Kansas) served as the GPDN regional Training and Education Coordinator and serves on the National Training and Education Committee (Rachel McCarthy, Chair). State training coordinators are Ana Cristina Fulladolsa (CO), Jim Stack & Judy O'Mara (KS), Mary Burrows (MT), Kyle Broderick (NE), Jesse Ostrander (ND), Jen Olson (OK), Emmanuel Byamukama (SD), Ken Obasa (TX), and William Stump (WY). How have the results been disseminated to communities of interest? Summary of publications and presentations resulting from training efforts and summary of training/ First Detector highlights/success stories: A regional webinar series continued this year to provide GPDN states with the most recent information on several emerging and remerging pathogens and insect pests. First Detector training sessions for Master Gardeners were conducted in several GPDN states. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state estimated to have reached over 150,000 people. Summary of publications and presentations resulting from training efforts and summary of training/ First Detector highlights/success stories: A regional webinar series continued this year to provide GPDN states with the most recent information on several emerging and remerging pathogens and insect pests. First Detector training sessions for Master Gardeners were conducted in several GPDN states. A First Detector training occurred at the Cheyenne Botanical Gardens (WY) where Kyle Broderick (NE), Chandler Day (KS), and Dr. Whitney Cranshaw (CO). This training was in conjunction with Sentinel Plant Network (SPN) and the American Public Gardens Association where 35 individuals from 14 public garden within the Great Plains received pest awareness training. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state. How have the results been disseminated to communities of interest?Results were communicated to communities of interest through a variety of delivery vehicles including, web-based newsletters, conference presentations, town hall meeting, seminars, webinars, radio interviews, podcasts, and extension outreach and training programs. What do you plan to do during the next reporting period to accomplish the goals?Description of Activities to be performed: 1. Detection: Each of the GPDN states is encouraged to conduct First Detector training in their respective states and register it in the First Detector website. Each state is also encouraged to recommend the First Detector online training modules to get First Detectors registered by completing modules. Coordination with other agencies is required in cases of certain pests of consequence as determined by state SPROs and SPHDs. GPDN member states are encouraged to participate in exercises if time allows or if USDA performs an exercise in their state as determined by the USDA-APHIS-PPQ Training office. GPDN will encourage Regional Center staff and member states' personnel to participate in training and professional development opportunities (e.g., workshops, seminars, etc.) as they become available and as resources allow. 2. Diagnosis: Submission of all diagnostic laboratory data (disease, insect pest) will continue. Some GPDN states contribute data from secondary pathology, entomology, and nematology laboratories. Data submission to the NPDN Data Repository is required for member laboratories in the NPDN. If supplementary funding is secured, we will support and encourage GPDN diagnosticians to attend training available from APHIS, universities and others. We will coordinate sample movement and surge support, and provide all updated SOPs and protocols on a timely basis. GPDN members will review, update, and/or author new SOPs as needed. During this project year (1 September 2019 - 31 August 2020), GPDN will continue to support the development of the new NPDN laboratory accreditation system. 3. Reporting: The GPDN Regional Center (RC) will ensure that all GPDN member labs maintain the necessary infrastructure to support reliable and secure communication capability. The GPDN RC will maintain the exercise module of PDIS, support the national and regional web portal, and contribute to the development of the IT annual plans of work and strategic plan updates. GPDN will continue to provide IT support to GPDN, NEPDN, and NCPDN through the PDIS software system. PDIS is the program for the diagnostics databases and connections between state, regional, and national databases. GPDN will continue to archive the web-based seminar series on the regional web site. Pending the availability of funding, the GPDN Regional Exercise Coordinator will support the national exercise program for diseases and pests of concern, in collaboration with APHIS and other entities (e.g., States' Department of Agriculture, Forest Service, etc.). The GPDN web portal was updated last year and will be managed in an effort to remain relevant and useful to members and clientele. GPDN Regional Center leadership will continue to serve in present roles on Committees with NPDN and the region. GPDN Regional Center staff will perform the following functions: Provide state partners with protocols for entering data to the National Repository. Provide advice for purchase of equipment for contemporary diagnostic work. Develop and distribute First Detector resources. Schedule training opportunities for regional staff with expert labs at APHIS or elsewhere as need arise. Represent the Great Plains Region nationally where regional interests are at stake. Prepare, test, and distribute standard operating procedures to enable advanced diagnostic tests for significant pests and pathogens. GPDN Regional Center staff will contribute to the greater good of the National Network by: Serving on the Executive, Associate Director, Operations, IT, Exercise, Training and Education, PDIS Change-Management, and Strategic Planning Committees. Keeping regional center staff in compliance with state and federal regulations e.g. inter- and intra-state transport of potentially harmful (to agricultural commodities) pests and pathogens. Maintaining the primary dedicated server for critical Network data stored at Kansas State University and backed up atPurdue University (CERIS). Ensuring that the GPDN Regional Center laboratory and staff are prepared to provide surge support to other regions when needed and that arrangements are in place to obtain surge support in the event that the Great Plains Region is overwhelmed with unexpected samples. The GPDN Director will remain an active member of the NPDN Executive Committee. The GPDN Director and Associate Director will remain active members of the NPDN Operations Committee and will: contribute to the implementation of the NPDN strategic plan, contribute to the national and regional annual plans of work, serve on regional and national committees, and when needed, provide oversight to regional and national committees. Where and when funding allows, GPDN members will continue to provide first detector training through a variety of vehicles common across the Great Plains Region. GPDN will partner with APHIS PPQ disease in and pest outbreak preparedness exercises. GPDN will contribute to the national plan for engagement of the research community to support the NPDN mission and strategic plan. GPDN will promote Network partnerships with research programs that interface with plant health, outbreak detection and pathogen and pest diagnostics. GPDN will contribute to and support public relations events and activities consistent with the NPDN mission. GPDN staff will participate in annual Town Hall Meetings.
Impacts
What was accomplished under these goals?
Summary of Accomplishments and Regional Highlights Detection: Detection accomplishment in the GPDN included, but were not limited to, Colorado detected Alfalfa mosaic virus in pepper (Capsicum annum), Botrytis cinerea in hemp (Cannabis sativa), Setomelanomma holmii in spruce (Picea pungens), Hop latent viroid in hemp (Cannabis sativa), and Parthenothrips dracaena on Schefflera sp. Kansas provided diagnostic support to the Kansas Department of Agricultural boxwood blight survey and trace forwards/back for Phytophthora ramorum infect nursery stock. Montana detected Globe thistle (Echinops schaerocephalus), Partridge pea (Chamaecrista fasciculata), Botrytis euroamericana and Botrytis prunorum found on chickpea (Cicer arietinum), white rust on mustard (Albuga candida) and could be an important pathogen of canola. Three bed bug workshops were conducted where 82% of participants increased their knowledge about detecting bed bug infestations, 86% increased their knowledge about bed bug biology, and 73% were likely to make monitoring/inspection changes for bed bugs based of the information learned at the workshops. Nebraska taught graduate and undergraduate students to look at and diagnose plant health problems. The graduate students gained a better understanding of economically important plant pathogens and the ability to diagnose plants in the field. North Dakota detected Boxwood Blight (Calonectria pseudonaviculata) and passed an audit for Cms testing for potato bacterial ring rot entry into Canada. Oklahoma worked with Oklahoma Department of Agriculture to detect Phytophthora ramorum in a large local nursery and garden centers and detected brown marmorated stink bug (Halyomorpha halys), American Plum Line Pattern Virus, Little Cherry 2 virus, and Hammerhead Flatworm (Bipalium sp.). A Direct Trapping of Viruses on Plastic method was taught in a hands-on workshop to 16 members of the GPDN in a workshop in October 2019 and a webinar with instructional videos was presented as part of the GPDN webinar series on February 12, 2020. South Dakota detected Phytophthora sansomeana on soybeans, bacterial leaf streak (Xanthomonas vasicola pv. Vasculorum) on corn. Texas identified 13 candidate bacterial consortia from corn samples collected from Texas, Kansas, and Colorado states with potential for mitigation of fumonisin production in corn. Wyoming had a first detectors training at the Cheyenne Botanical Gardens that was hosted in conjunction with the Sentinel Plant Network. Diagnosis: GPDN accomplishments in diagnosis included, but were not limited to, Colorado adopted a PCR assay from pesticded-coated seeds to detect Synchytrium endobioticum in tomato seeds. Ditylenchus dipsaci was able to be diagnosed using newly adpoted nematode extraction and identification methods. A PCR bacterial isolation assay from seed was optimized to diagnose Pseudomonas cannabina in hemp seed and Xanthomonas cannabis detection in hemp seed. A freezer blotter method was adopted to diagnose Fusarium sp. detection in hemp seed using fungal isolation, PCR of the ITS region and subsequent Sanger sequencing were used to identify the species. The same freezer blotter method was adopted to detect Leptosphaeria woroninii in hemp seed. A method for DNA extraction and PCR assay from hemp seeds was developed to detect Ramularia collo-cygni in hemp seed. A method combining qPCR and a tomato bioassay was developed and adopted for Spongospora subterranea detection in potting mix.A high-throughput method for DNA extraction and PCR assay from hemp leaf tissue was developed for Beet curly top virus detection in hemp. An optimized (by another diagnostic lab) method based on the RT-PCR assay to detect Hop latent viroid in hemp. Kansas developed two SOP for handling and processing hemp samples and sudden oak death survey samples. An additional 1,330 digital samples were address between the diagnostic lab and the horticulture rapid response center. Montana saved over $4.9 million affecting approximately 190,000 acres as a result of SDL recommendations. Nebraska serves as the Nebraska Department of Agriculture's diagnostic resource for nursery and crop inspectors and is increasing their diagnostic capabilities by purchasing a PCR workstation. North Dakota helps agricultural producers and professionals, horticulture and urban forestry professionals, university researchers and homeowners identify plant pests, one of the keys of Integrated Pest Management. They provided management advice regarding plant pathogens or diseases for approximately 500 disease identification requests and processed approximately 400 insect identification requests, of which approximately 90% of submitters were receptive to implementing appropriate integrated pest management strategies to control their respective insect pests. They also have started testing soil for Pot Mop-Top Virus. Oklahoma adopted a new SOP for detection of Meloidogyne spp. and M. enterolobii. This is a combination of traditional nematode extraction using sugar flotation centrifugation and molecular tests on individual nematodes. An 18-month evaluation was successful validated for Direct Trapping of Viruses on Plastic that it is effective for rose rosette virus, cucumber mosaic, Poty virus group, tobacco rattle and tobacco mosaic viruses. The method has reduced costs of DNA extraction by about 65%. An AmpliFire from Agdia was purchased and has been successfully used to test for Xylella fastidiosa and Grapevine Red Blotch Virus. An experimental protocol from researchers using isothermal amplification equipment is being used for a project on Rose rosette virus (RRV) and continuing to be evaluated. Overall the AmpliFire has reduced the time for testing RRV samples from 6-8 hours down to 30 minutes and the cost of the test is also much lower as a result of less expensive reagents and reduced labor. South Dakota identified a first detection of Phytophthora sansomeana, a new root rot pathogen of soybean in South Dakota. Texas added mycotoxin testing to the services now offered by the lab for the following mycotoxins - DON, Fumonisin, Aflatoxin, Ochratoxin, Zearalenone, and T-2/HT-2 and is accredited to offer mycotoxin testing services by the Office of the Texas State Chemist. Wyoming diagnosed 41 submitted samples from a wide range of plant types to include ornamental plants, field crops, turf, vegetables and fruits. The following pest categories diagnoses were found; arthropods 4, pathogens 16, abiotic disorders 14, nematodes 4, and 3 others. Reporting: GPDN accomplishments in reporting included, but were not limited to, Kansas' real time deposits into the National repository. Kansas also processed 612 samples and diagnosed 115 abiotic disorders, 26 arthropods, 116 nematodes, 300 pathogens, and 1 plant/weed. Colorado processed 704 samples and diagnosed 48 abiotic disorders, 62 arthropods, 6 nematodes, 191 pathogens, and 5 plants/weeds. Montana processed 1,373 samples and diagnosed 407 abiotic disorders, 576 arthropods, 2 nematodes, 641 pathogens, 235 plants/weeds. Nebraska providing weekly updates to the Crop Watch newsletter and diagnosed 96 abiotic disorders, 57 arthropods, 14 nematodes, and 685 pathogens. North Dakota processed 2,341 and diagnosed 75 abiotic disorders, 243 arthropods, 910 nematodes. 486 pathogens, and 17 plants/weeds. Oklahoma uploaded all data to the NPDN repository. For this time period, they uploaded data for 3,741 samples. South Dakota processed 117 samples and diagnosed 28 abiotic disorders, 16 arthropods, 21 nematodes, 107 pathogens, and 6 plants/weeds. Texas processed 77 samples and diagnosed 171 pathogens, 17 arthropods, and 26 abiotic disorders. Wyoming processed 18 sample and diagnosed 41 pests.
Publications
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Dr. Todd Gaines Colorado State University 01/15/2020 Amaranth Biology & Identification
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Dr. Neil McRoberts UC Davis 01/22/2020 The information content of diagnostics
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Dr. Nicole Ward Gauthier University of Kentucky 01/29/2020 Crop rotations & potential pathogen carry-over in hemp
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Dr. Raymond Cloyd Kansas State University 02/05/2020 Insect and Mite Identification in Greenhouses
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Mrs. Jen Olson and Dr. Francisco Ochoa-Corona Oklahoma State University 02/12/2020 Direct trapping of plant viruses
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Dr. Alison Brennan Montana State University 02/19/2020 Recommendations and Resources for Responding to Distressed Producers
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Dr. Dawn Gouge University of Arizona 02/26/2020 The Perfect Ectoparasite- Bed Bugs and IPM
- Type:
Websites
Status:
Published
Year Published:
2020
Citation:
Mr. Ryan Armbrust Kansas Forest Service 03/04/2020 Community Tree Diversity: Building Resilience Against Invasive Insects, Diseases, and Plants
|
Progress 09/01/18 to 08/31/19
Outputs
Target Audience:Target audiences reached this past year included large and small-scale agricultural producers, homeowners, plant production and nursery operators, diagnosticians, state (Departments of Agriculture) and federal (USDA APHIS Plant Protection and Quarantine) plant regulatory personnel, and plant industry (nursery, seed) support professionals. The Great Plains Diagnostic Network (GPDN) is one of five regional networks of the National Plant Diagnsotic Network and is comprised of representatives of Land Grant Universities and Agricultural Experiment Stations from the following nine states; Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming. GPDN is comprised of a team of specialists skilled at rapid and accurate identification of plant pests and pathogens, timely communicationsof diagnostic test results, and the delivery of training programs to facilitate early detection and mitigation of pest and disease outbreaks that impact large and small-scale agrilcutultural production systems, commercial horticultural production and retail systems, as well as homeowner environments. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Total number of training sessions and total number of First Detectors trained: GPDN members conducted many training sessions for First Detectors. The number of sessions conducted and the number of First Detectors trained are included in the total numbers presented in Table 1. At least 3,109 First Detectors received some first detector training at with least 60 presentations/publications. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Summary of regional training efforts: Christian Webb (Kansas) served as the GPDN regional Training and Education Coordinator and serves on the National Training and Education Committee (Rachel McCarthy, Chair). State training coordinators are Ana Cristina Fulladolsa (CO), Jim Stack & Judy O'Mara (KS), Mary Burrows (MT), Kyle Broderick (NE), Jesse Ostrander (ND), Jen Olson (OK), Emmanuel Byamukama (SD), Ken Obasa (TX), and William Stump (WY). How have the results been disseminated to communities of interest?Summary of publications and presentations resulting from training efforts and summary of training/ First Detector highlights/success stories: A regional webinar series continued this year to provide GPDN states with the most recent information on several emerging and remerging pathogens and insect pests. First Detector training sessions for Master Gardeners were conducted in several GPDN states. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state estimated to have reached over 150,000 people. Summary of publications and presentations resulting from training efforts and summary of training/ First Detector highlights/success stories: A regional webinar series continued this year to provide GPDN states with the most recent information on several emerging and remerging pathogens and insect pests. First Detector training sessions for Master Gardeners were conducted in several GPDN states. A First Detector training occurred at the Cheyenne Botanical Gardens (WY) where Kyle Broderick (NE), Chandler Day (KS), and Dr. Whitney Cranshaw (CO). This training was in conjunction with Sentinel Plant Network (SPN) and the American Public Gardens Association where 35 individuals from 14 public garden within the Great Plains received pest awareness training. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state. What do you plan to do during the next reporting period to accomplish the goals?Description of Activities to be performed: 1. Detection: Each of the GPDN states is encouraged to conduct First Detector training in their respective states and register it in the First Detector website. Each state is also encouraged to recommend the First Detector online training modules to get First Detectors registered by completing modules. Coordination with other agencies is required in cases of certain pests of consequence as determined by state SPROs and SPHDs. GPDN member states are encouraged to participate in exercises if time allows or if USDA performs an exercise in their state as determined by the USDA-APHIS-PPQ Training office. GPDN will encourage Regional Center staff and member states' personnel to participate in training and professional development opportunities (e.g., workshops, seminars, etc.) as they become available and as resources allow. 2. Diagnosis: Submission of all diagnostic laboratory data (disease, insect pest) will continue. Some GPDN states contribute data from secondary pathology, entomology, and nematology laboratories. Data submission to the NPDN Data Repository is required for member laboratories in the NPDN. If supplementary funding is secured, we will support and encourage GPDN diagnosticians to attend training available from APHIS, universities and others. We will coordinate sample movement and surge support, and provide all updated SOPs and protocols on a timely basis. GPDN members will review, update, and/or author new SOPs as needed. During this project year (1 September 2019 - 31 August 2020), GPDN will continue to support the development of the new NPDN laboratory accreditation system. 3. Reporting: The GPDN Regional Center (RC) will ensure that all GPDN member labs maintain the necessary infrastructure to support reliable and secure communication capability. The GPDN RC will maintain the exercise module of PDIS, support the national and regional web portal, and contribute to the development of the IT annual plans of work and strategic plan updates. GPDN will continue to provide IT support to GPDN, NEPDN, and NCPDN through the PDIS software system. PDIS is the program for the diagnostics databases and connections between state, regional, and national databases. GPDN will continue to archive the web-based seminar series on the regional web site. Pending the availability of funding, the GPDN Regional Exercise Coordinator will support the national exercise program for diseases and pests of concern, in collaboration with APHIS and other entities (e.g., States' Department of Agriculture, Forest Service, etc.). The GPDN web portal was updated last year and will be managed in an effort to remain relevant and useful to members and clientele. GPDN Regional Center leadership will continue to serve in present roles on Committees with NPDN and the region. GPDN Regional Center staff will perform the following functions: 1. Provide state partners with protocols for entering data to the National Repository. 2. Provide advice for purchase of equipment for contemporary diagnostic work. 3. Develop and distribute First Detector resources. 4. Schedule training opportunities for regional staff with expert labs at APHIS or elsewhere as need arise. 5. Represent the Great Plains Region nationally where regional interests are at stake. 6. Prepare, test, and distribute standard operating procedures to enable advanced diagnostic tests for significant pests and pathogens. GPDN Regional Center staff will contribute to the greater good of the National Network by: 1. Serving on the Executive, Associate Director, Operations, IT, Exercise, Training and Education, PDIS Change-Management, and Strategic Planning Committees. 2. Keeping regional center staff in compliance with state and federal regulations e.g. inter- and intra-state transport of potentially harmful (to agricultural commodities) pests and pathogens. 3. Maintaining the primary dedicated server for critical Network data stored at Kansas State University and backed up at Purdue University (CERIS). 4. Ensuring that the GPDN Regional Center laboratory and staff are prepared to provide surge support to other regions when needed and that arrangements are in place to obtain surge support in the event that the Great Plains Region is overwhelmed with unexpected samples. The GPDN Director will remain an active member of the NPDN Executive Committee. The GPDN Director and Associate Director will remain active members of the NPDN Operations Committee and will: 1) contribute to the implementation of the NPDN strategic plan, 2) contribute to the national and regional annual plans of work, 3) serve on regional and national committees, and 4) when needed, provide oversight to regional and national committees. Where and when funding allows, GPDN members will continue to provide first detector training through a variety of vehicles common across the Great Plains Region. GPDN will partner with APHIS PPQ disease in and pest outbreak preparedness exercises. GPDN will contribute to the national plan for engagement of the research community to support the NPDN mission and strategic plan. GPDN will promote Network partnerships with research programs that interface with plant health, outbreak detection and pathogen and pest diagnostics. GPDN will contribute to and support public relations events and activities consistent with the NPDN mission. GPDN staff will participate in annual Town Hall Meetings.
Impacts
What was accomplished under these goals?
Summary of Accomplishments and Regional Highlights Detection: Detection accomplishment in the GPDN included, but were not limited to, Colorado continuing to offer specific potato pathogen detection services (Dickeya sp., Pectobacterium sp., Colletotrichum coccodes, Clavibacter michiganensis, Spongospora subterranea, PVY, PMTV, Ralstonia solanacearum) for the potato industry. Diagnostic reports were used for quality assessments and/or to make management decisions. Kansas provided diagnostic support to the 2018 Kansas Department of Agricultural spring ornamental nursery survey. Diseases detected included, Botrytis blight on many ornamentals, Canna Yellow Mottle Virus, tospo viruses, Hosta Virus x, Rose Mosaic Virus, Rose Rosette Virus and Fusarium wilt in mums. Montana hosted workshops on beg bug management and grass identification with attendees reporting 65% are likely making bed bug monitoring and management changes based on the new knowledge and 89% of participants described their ability to identify invasive annual grasses as either moderately able to very able. Nebraska accompanied 21 graduate students on a tour across the state of Nebraska looking at and diagnosing plant health problems. The graduate students gained a better understanding of economically important plant pathogens and the ability to diagnose plants in the field. Oklahoma taught extension educators in full-day of trainings in Entomology and Plant Pathology. This was the first full EPP training event for about 25% of the educators. Pre and post test scores showed significant improvement in knowledge in all areas of Entomology and Plant Pathology. South Dakota trained CAPS survey staff and IPM Field School participants. Participants gained knowledge to confidently identify target diseases in small grains and were equipped to perform quick plant disease identifications by group - fungi, bacterial and viral diseases, respectively. Wyoming participated in a UG99 CAPS survey. 280 winter wheat and barley samples were evaluated for stem rust. Stem rust was not detected. Diagnosis: GPDN accomplishments in diagnosis included, but were not limited to, Colorado finding Erwinia aphidicola on lupine plants grown in a CO greenhouse. The clinic worked with the Beltsville APHIS-PPQ laboratory to identify the pathogen. Infected plants were destroyed. Since, the clinic has discussed collaborating with the Beltsville Lab to develop a PCR-based assay for rapid detection of E. aphidicola. Kansas developed two new assays for the detection of high consequence pathogens the wheat blast pathogen, Magnaporthe oryzae Triticum, and Rathayibacter toxicus populations. These new diagnostic assays will facilitate the timely detection of these pathogens. Montana had one phone survey respondent claim $6 million was saved ($63/a) as a result of a sample submitted to the diagnostic lab, and over $7.5 million was saved affecting approximately 408,000 acres as a result of SDL recommendations. Nebraska serves as the Nebraska Department of Agriculture's diagnostic resource for nursery and crop inspectors, and over 500 samples were inspected for phytosanitary inspection. Oklahoma tested 2,526 stone fruit samples for the Oklahoma Stone Fruit Commodity Farm Bill Survey. There were no detections of PPV or Phytoplasma diseases. South Dakota identified a first detection of Phytophthora sansomeana, a new root rot pathogen of soybean in South Dakota. Texas diagnosed 53 samples from a wide range of plant types to include ornamental plants, field crops, trees, vegetables and fruits. The following pest categories diagnoses were found; arthropods-7 confirmed, pathogens-44 confirmed, abiotic disorders-1 confirmed and 1 suspected. Wyoming provided pest and disorder diagnostics for state clientele. They diagnosed 188 submitted samples from a wide range of plant types to include ornamental plants, field crops, turf, vegetables and fruits. The following pest categories diagnoses were found; arthropods 74, pathogens 52, abiotic disorders 58, weeds and plants 3. Reporting: GPDN accomplishments in reporting included, but were not limited to, Kansas' real time deposits into the National repository. Of the 768 national repository uploads from 2018, over 80% of the diagnoses were sent within 14 days and 93.6% were deposited within the 30 days. These timely uploads supported the real time integrity of the NPDN repository. Montana submitted 2967 entries of plant disease, pest and abiotic results to the NPDN National Repository. Nebraska providing weekly updates to the Crop Watch newsletter. Producers and field scouts knew what diseases to prepare for in their given region. Oklahoma uploaded all data to the NPDN repository. For this time period, they uploaded data for 5,457 samples. South Dakota submitted real-time entries of 636 plant and pest diagnostic results to the NPDN National Repository. Texas informed producers and collaborators of an important bacterial disease of wheat based on samples diagnosed in the laboratory. Growers prepared for and surveyed their fields for the disease; collaborators are now trained as scouts for this disease. Wyoming surveyed the Western Sugar Cooperative sugar beet production area for fungicide resistance. Western sugar adopts production practices to mitigate fungicide resistance problems.
Publications
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Dr. Tamra Jackson-Ziems University of Nebraska Lincoln 1/30/2019 Bacterial Leaf Streak of Corn: Just Another Zebra in Nebraska
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Carmen Murphy Montana State University 2/06/2019 Root Rot of Pulse Crops
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Uta McKelvy Montana State University 2/20/2019 Disease Forecasting Models: Concepts, challenges, and example
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Dr. Nina Zidack Montana State University 2/27/2019 Certifying seed potatoes in Montana and beyond
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Dr. Nicole Ward Gauthier University of Kentucky 3/06/2019 New Friends and Old Foes: Hemp Disease Sightings in KY
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Dr. James Stack Kansas State University 3/27/2019 NPDN Strategic Planning: What have we done? Where are we at? Where are we going?
- Type:
Websites
Status:
Published
Year Published:
2019
Citation:
Dr. Michelle Flenniken Montana State University 1/23/2019 What's Killing the Bees? The Impacts of Pathogens and other Factors on Honey Bees
|
Progress 09/01/17 to 08/31/18
Outputs
Target Audience:The Great Plains Diagnostic Network (GPDN) is one of five regional networks of NPDN and is comprised of representatives of Land Grant Universities and Agricultural Experiment Stations from the following nine states; Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming. GPDN is a team of specialists skilled at rapid and accurate identification of plant pests and pathogens and the delivery of training programs to facilitate early detection of outbreaks. Target audiences reached this past year include large and small-scale agricultural producers, homeowners, plant production and nursery operators, diagnosticians, state and federal plant regulatory personnel, and plant industry support professionals. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Through NPDN and its partnership with USDA APHIS PPQ, NPDN diagnosticians are provided an array of training and professional development opportunities including advanced diagnsotic workshops to learn new technologies and protocols approved for high consequence pathogens and pests. How have the results been disseminated to communities of interest?Through a variety of distribution systems including standard and social media platforms, including radio, television, webinars, Facebook, Twitter, etc. What do you plan to do during the next reporting period to accomplish the goals?1.Detection: Each of the GPDN states is encouraged to conduct First Detector training in their respective states and register it in the First Detector website. Each state is also encouraged to recommend the First Detector online training modules to get First Detectors registered by completing modules. Coordination with other agencies is required in cases of certain pests of consequence as determined by state SPROs and SPHDs. GPDN member states are encouraged to participate in exercises if time allows or if USDA performs an exercise in their state as determined by the USDA-APHIS-PPQ Training office. 2. Diagnosis: Submission of all diagnostic laboratory data (disease, insect pest) will continue. Some GPDN states contribute data from secondary pathology, entomology, and nematology laboratories. Data submission to the NPDN Data Repository is required for member laboratories in the NPDN. If supplementary funding is secured, we will support and encourage GPDN diagnosticians to attend training available from APHIS, universities and others. We will coordinate sample movement and surge support, and provide all updated SOPs and protocols on a timely basis. GPDN members will review, update, and/or author new SOPs as needed. During this project year (1 September 2017 - 31 August 2018), we will support the STAR-D laboratory accreditation system as it develops. The GPDN Regional Center Diagnostic Laboratory will submit to an audit when the STAR-D program is ready. The GPDN Director and regional staff will update and disseminate the current draft STAR-D quality manual and auditing checklist. The GPDN Regional Center has three trained STAR-D Audit/Auditors, Ron French, Judy O'Mara and Sharon Dobesh. The GPDN Regional Center Laboratory will participate in discussions and planning for implementation of the Star-D program. 3. Reporting: The GPDN Regional Center (RC) will ensure that all GPDN member labs maintain the necessary infrastructure to support reliable and secure communication capability. The GPDN RC will maintain the exercise module of PDIS, support the national and regional web portal, and contribute to the development of the IT annual plans of work and strategic plan updates. GPDN will continue to provide IT support to GPDN, NEPDN, and NCPDN through the PDIS software system. PDIS is the program for the diagnostics databases and connections between state, regional, and national databases. GPDN will continue to archive the web-based seminar series on the regional web site. The GPDN Regional Exercise Coordinator will assist with national exercises and coordinate regional exercises for diseases and pests of concern, in collaboration with APHIS and other entities (i.e. Forest Service, etc). There are limited exercises planned for 2018-2019. The GPDN web portal was updated last year and will be managed in an effort to remain relevant and useful to members and clientele. GPDN Regional Center leadership will continue to serve in present roles on Committees with NPDN and the region. GPDN Regional Center staff will perform the following functions: 1. Provide state partners with protocols for entering data to the National Repository. 2. Provide advice for purchase of equipment for contemporary diagnostic work. 3. Develop and distribute First Detector resources. 4. Schedule training opportunities for regional staff with expert labs at APHIS or elsewhere as need arise. 5. Represent the Great Plains Region nationally where regional interests are at stake. 6. Prepare, test, and distribute standard operating procedures to enable advanced diagnostic tests for significant pests and pathogens. GPDN Regional Center staff will contribute to the greater good of the National Network by: 1. Serving on the Executive, Associate Director, Operations, IT, Exercise, Training and Education, and PDIS Change-Management Committees. 2. Keeping regional center staff in compliance with state and federal regulations e.g. inter- and intra-state transport of potentially harmful (to agricultural commodities) pests and pathogens. 3. Maintaining the primary dedicated server for critical Network data stored at Kansas State University and backed up at Purdue University (CERIS). 4. Ensuring that the GPDN Regional Center laboratory and staff are prepared to provide surge support to other regions when needed and that arrangements are in place to obtain surge support in the event that the Great Plains Region is overwhelmed with unexpected samples. The GPDN Director currently serves as the Executive Director of NPDN and will remain an active member of the NPDN Executive Committee. The GPDN Director and Associate Director will remain active members of the NPDN Operations Committee and will: 1) contribute to the implementation of the NPDN strategic plan, 2) contribute to the national and regional annual plans of work, 3) serve on regional and national committees, and 4) when needed, provide oversight to regional and national committees. Where and when funding allows, GPDN members will continue to provide first detector training through a variety of vehicles common across the Great Plains Region. GPDN will partner with APHIS PPQ in outbreak preparedness exercises. GPDN will contribute to the national plan for engagement of the research community to support the NPDN mission and strategic plan. GPDN will promote Network partnerships with research programs that interface with plant health, outbreak detection and pathogen and pest diagnostics. GPDN will contribute to and support public relations events and activities consistent with the NPDN mission. GPDN staff will participate in the annual Town Hall Meeting.
Impacts
What was accomplished under these goals?
State: CO KS MT NE ND OK SD TX WY Totals: # of non-FD presentation/publications: 4 63 51 2 11 21 5 10 41 208 Number of non-FD attendees: 352 1,915 134,211 21 1810 176,065 50 833 854 316,111 # of FD presentation/publications: o 0 86 16 0 36 2 1 0 141 Number of FD attendees: o 0 702 1252 0 1,222 85 18 0 3,279 Grand total presentation/publications: 4 63 136 18 11 57 7 11 41 348 Grand total attendees: 352 1,915 134,913 1273 1810 177,287 135 851 854 319,390 Pieces of new equipment: 0 0 0 0 0 0 0 0 0 0 Professional development conferences attended: 3 8 15 6 1 25 4 3 4 69 Number of other activities: 6 3 7 3 1 16 0 3 1 40 Number of samples processed: 192 1,520 1,035 1,486 3,846 5,548 568 1,209 647 16,051 Number of organism found: pathogen: 133 1101 774 701 2203 3669 352 6 35 8,974 arthropods 7 31 703 560 128 943 10 4 227 2,613 plants/weeds 1 5 575 17 39 50 13 0 1 701 nematode: 11 110 7 163 1,255 428 739 0 0 2,713 abiotic: 5 151 346 45 99 359 95 6 0 1,106 other: 0 0 3 0 122 99 0 0 0 224 Total number of organisms found: 157 1398 2408 1486 3846 5548 1209 16 263 16,331 Type of technique used: bioassay: 0 0 0 0 3 6 0 0 0 9 biochemical: 0 0 0 0 1 86 0 0 0 87 culture: 5 11 6 135 56 278 40 35 0 566 electron microscopy: 0 0 9 0 1 0 0 0 0 10 image: 3 4 56 180 4 740 20 0 0 1,007 incubation: 51 1 455 40 273 98 144 0 7 1,069 inclusion Body: 0 0 0 0 1 0 0 0 0 1 microscopic: 79 386 1273 648 247 1670 214 648 54 5,219 molecular analysis: 0 2 0 0 128 12 0 0 0 142 nematode extraction: 3 97 0 163 1,883 425 739 0 3 3,313 PCR: 75 46 3 67 1789 605 0 6,026 0 8,611 serological: 156 805 55 101 323 2453 101 2632 0 6,626 soil analysis: 0 0 0 19 8 0 0 27 visual observation: 1 2 0 0 3 8 0 0 0 14 Total number of techniques used: 15 166 1366 152 461 418 24 687 99 3,388
Publications
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Progress 09/01/16 to 08/31/17
Outputs
Target Audience:Target Audience Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming Efforts- Pesticide applicator trainings, newsletter articles, producer meetings, lab diagnostics, radio segments, pest alerts, meeting posters and presentations, press releases. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Diagnosticians from several GPDN states received advanced training through an array of formats from hands-on workshops and symposia. GPDN members attended training programs sponsored by NPDN and by USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory. These training sessions focused on molecular and morphological identification techniques for high consequence pathogens. The training sessions were conducted by USDA-APHIS experts at the federal laboratory in Beltsville, Maryland. Diagnosticians throughout the Great Plains Region and the NPDN increased their knowledge of the diagnosis of high consequence plant pathogens and insect pests through lecture based seminars delivered in person or over the web by use of Adobe Connect (through Montana State University) and Zoom (through Kansas State University) and the GPDN Webinar series. How have the results been disseminated to communities of interest?GPDN members (diagnosticians and extension specialists) presented and published material focusing on general plant pathology, diagnosing plant problems, GPDN and NPDN, pest emergencies, fungicide and pesticide resistance management, and specific plant disease and pest issues. GPDN members conducted many training sessions on an array of pests and diseases for First Detectors, although at a reduced rate due to budget reductions. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Attendees included individuals from grower organizations, crop consultants, master gardeners, first detectors, horticultural society members and plant pathologists/entomologists. What do you plan to do during the next reporting period to accomplish the goals?Diagnostics - All labs will maintain proper certifications and equipment maintenance to ensure quality diagnostics. GPDN labs will be required to upload all diagnostic data to the National Repository via PDIS. PDIS will continue to provide support and maintenance to the system and customer service to all our member and the 30-plus states that utilize PDIS as their lab management system. IT and Communications - All GPDN member states will be required to attend necessary GPDN and NPDN meetings and conference call as necessary. Participation will be expected of committee members. The GPDN regional center will participate in NPDN Operations Committee calls and the GPDN Director will participate in Executive Committee conference calls. GPDN will continue to provide leadership in the NPDN Exercise Preparedness Committee. GPDN will continue to provide oversight of the PDIS system and programmers. The PDIS programmers will in turn continue to work on an apps version of PDIS and provide support and maintenance to the PDIS system. Training and Education - All GPDN member states were encouraged to continue training and education as their budget allowed. If training was conducted, all First Detector trainings are to be reported in the national training and education website hosted at the University of Florida. Also all states are encouraged to recommend the online First Detector training modules at the online site for individuals interested in continuing first detector training or for use in state training programs.
Impacts
What was accomplished under these goals?
Major Activities Completed Governance and Network Coordination - Regional conference calls were convened on a regular basis to manage administrative issues, review policies, develop plans of work, and to discuss emerging issues. The GPDN Director and Associate Director developed and deployed the request for funding from GPDN member states and reviewed the submissions for consistency with the NPDN mission and project planning matrix. Annual reports of accomplishments were solicited, received, and reviewed. GPDN continued to provide leadership for the NPDN Exercise Committee and helping with National Outbreak Preparedness Exercise Program. Regional staff participated in all relevant network meetings. GPDN continued to provide the leadership and oversight for the Plant Diagnostic Information System (PDIS) in use in 30 states. Data Systems and Networking - GPDN continued to provide leadership and oversight for the Plant Diagnostic Information System (PDIS) in use in 30 states. The GPDN Regional Center Staff provided oversight for the process to develop, test, and implement the new applications-based version of PDIS. The PDIS team has been migrating PDIS from Oracle to Sequel and should complete the process in the next few months. GPDN continues to support the overall functioning of diagnostic systems. Diagnostics - GPDN state labs continued to upgrade, where possible their capabilities through the purchase of advanced diagnostic technology and to implement appropriate equipment maintenance programs that include scheduled certifications and record keeping. Progress was made toward the goal for all GPDN labs to have molecular diagnostic capability. Several diagnosticians have attended the trainings offered in Beltsville, MD to enhance their diagnostic certifications. Training & Education - GPDN labs should conduct First Detector training and when possible it should be reported into the National Education and Training Database. The NPDN on-line First Detector training system and modules should be utilized if appropriate for trainings. Attempts will be made to register First Detectors in the National Registry if possible. STAR-D - Many diagnosticians have taken advantage of STAR-D training opportunities (QMS training, Gap audit trainings, Document Round-up). Due to a better understanding of STAR-D from attending training(s), several states are now working on STAR-D Accreditation documents for future accreditation audits. Funding shortfalls continue to limit progrsss on laboratory accreditation.
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