Source: ACCELERATED AG TECHNOLOGIES, LLC submitted to NRP
DEVELOPMENT OF RIGOROUS AND RELIABLE METHODS TO PRESERVE MAIZE POLLEN
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1009790
Grant No.
2016-33610-25366
Cumulative Award Amt.
$99,769.00
Proposal No.
2016-00840
Multistate No.
(N/A)
Project Start Date
Aug 1, 2016
Project End Date
Mar 31, 2017
Grant Year
2016
Program Code
[8.2]- Plant Production and Protection-Biology
Recipient Organization
ACCELERATED AG TECHNOLOGIES, LLC
3051 104TH ST STE B
URBANDALE,IA 50322
Performing Department
(N/A)
Non Technical Summary
The seed industry generates tremendous value from hybrid crops relative to varietal or self-pollinated crops. For example, while corn (a hybrid crop) and soybeans (a self-pollinated crop) were planted on approximately the same land area in the U.S. in 2015, the total value of the corn hybrid seed industry was greater than 4x the value of the soybean seed industry. However, producing hybrid seed is expensive relative to self or open-pollinated crops. In some crops, such as soybeans and wheat, the biology of the plant is prohibitive to the economical production of hybrid seed, thus disabling the realization of the benefits of hybridity in these crops. Enabling a crop such as wheat to be produced as a hybrid would have an annual value exceeding several billion dollars.This project will develop methodsto extend the viability of pollen and enable on-demand use of the pollen. On-demand use of pollenin seed productionwill improve and enable hybrid seed production, thus improving agricultural productivity and food availability.
Animal Health Component
0%
Research Effort Categories
Basic
0%
Applied
0%
Developmental
100%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20515101060100%
Knowledge Area
205 - Plant Management Systems;

Subject Of Investigation
1510 - Corn;

Field Of Science
1060 - Biology (whole systems);
Goals / Objectives
1) Determine the major factors which affect the health of maize pollen 2) Determine the optimal conditions for storing maize pollen post conditioning 3) Determine the optimal combination of conditioning and storage conditions based on results from objectives 1) and 2) above
Project Methods
The major work focus for this project is divided into three specific categories concomitant with the technical objectives. Pollen conditioning protocols will be tested and adjusted to enable preservation (Technical Objective 1) followed by testing and optimization of storage parameters (Technical Objective 2), and lastly the best performing preservation conditions and storage conditions will be further optimized in combination with each other (Technical Objective 3). An array of germplasm will be utilized in the project, representing the genetic diversity present in U.S. germplasm.Field and greenhouse facilities are being leased that will be utilized to grow the experimental material. Regular plantings will occur over the course of the project to enable a constant supply of freshpollen (male inflorescence) and fertile silks (female inflorescence).

Progress 08/01/16 to 03/31/17

Outputs
Target Audience:AAT's primary audience are U.S. corn seed companies. We have had contact regarding our technology with 5 different companies comprising a significant portion of the market. Actual results have been reported to 3 companies under confidentiality agreements. Significant interest has been expressed, and we are following up to consider potential collaborations. Changes/Problems:There have been no major changes or problems in the project approach. Minor changes to entry lists, parameters tested, and other related changes that are within normal expectations for research projects of a biological nature. In addition, since there was a delay in funding, the major source of our experimental material shifted from the field to the greenhouse, which has affected some of our experiments. We still obtained pollen from the field, but it did not play as major of a role as expected. What opportunities for training and professional development has the project provided?The project has provided excellent training and professional development for AAT employees, to increase our expertise and competency in pollen handling and storage. No outside training or professional development has been provided yet as early patent filings are pending and are in the process of being written. How have the results been disseminated to communities of interest?Under confidentiality agreements, these results have been discussed with several seed companies that could be future customers. Dissemination via academic publications is planned once we have patents filed and methods perfected to the appropriate degree. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? For objective 1, we have defined precise parameters for maintaining maximum pollen health. These parameters form the basis for the patent application that has been filed. For objective 2, we have defined conditions for short and long term storage of pollen, post-conditioning. These parameters also form the basis for the patent application that has been filed. For objective 3, combinations of objectives 1 and 2 have been tested and protocols that work across 18 of 21 genotypes of corn have been defined. ?

Publications

  • Type: Other Status: Other Year Published: 2017 Citation: No publications, but I cannot move on unless I fill in these fields


Progress 08/01/16 to 03/31/17

Outputs
Target Audience:AAT's primary audience are U.S. corn seed companies. We have had contact regarding our technology with 5 different companies comprising a significant portion of the market. Actual results have been reported to 3 companies under confidentiality agreements. Significant interest has been expressed, and we are following up to consider potential collaborations. Changes/Problems:There have been no major changes or problems in the project approach. Minor changes to entry lists, parameters tested, and other related changes that are within normal expectations for research projects of a biological nature. In addition, since there was a delay in funding, the major source of our experimental material shifted from the field to the greenhouse, which has affected some of our experiments. We still obtained pollen from the field, but it did not play as major of a role as expected. What opportunities for training and professional development has the project provided?The project has provided excellent training and professional development for AAT employees, to increase our expertise and competency in pollen handling and storage. No outside training or professional development has been provided yet as early patent filings are pending and are in the process of being written. How have the results been disseminated to communities of interest?Under confidentiality agreements, these results have been discussed with several seed companies that could be future customers. Dissemination via academic publications is planned once we have patents filed and perfected to the appropriate degree. What do you plan to do during the next reporting period to accomplish the goals?Several experiments are in progress and planned to finish objective 2 and 3. These experiments should be completed by the funding deadline of March 30, but there could be a few more follow up experiments that may cause us to file for an extension, although there will not be any funding left. Any extended SBIR work would be funded from other sources.

Impacts
What was accomplished under these goals? For objective 1, we have defined precise parameters for maintaining maximum pollen health. These parameters form the basis for the provisional patent application that has been filed. For objective 2, we have defined conditions for long term storage of pollen. These parameters form the basis for the provisional patent application that has been filed, but more work is still in progress to further optimize the details and assess if it works across corn genotypes. For objective 3, combinations of objectives 1 and 2 have been tested and are in the process of being evaluated at the time of this report.

Publications

  • Type: Other Status: Other Year Published: 2020 Citation: Not applicable: The system would not let me move on without putting something in for publications. We do not have any yet.