Progress 01/14/16 to 09/30/20
Outputs
Target Audience:Using various sources (publications, classes, workshops, symposium and conferences), I was able to reach a wide audience (scientific communities, students, faculty, industry and producers) and hope that will have a large geographic influence and impact. Changes/Problems:Our approach was original and different as we targeted dsRNA (new class on non-coding RNA). Most of studies used coding RNA. We developped a new strategy to isolate and study dsRNA. What opportunities for training and professional development has the project provided?Training activity: mentoring 2 PhD students and trained one program associate How have the results been disseminated to communities of interest?The results have been published in different journals including American Journal of Pathology which has an impact factor of 4.069. This journal is published by Elsevier on behalf of the American Society for Investigative Pathology. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
During this period of the project, we made a breakthrough by identifying a molecular mechanism by which Staphylococcus induce bone degeneration in BCO chickens. Specifically, we found that bacterial infection reduces Dicer 1 expression and leads to the accumulation of double-stranded RNA which in turn activates NLRP3 inflammasome and increases proinflammatory cytokine production leading to bone cell death.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
2. Adnan A. K. Alrubaye, Nnamdi S. Ekesi, Amer Hasan, Ethan Elkins, Sohita Ojha, Sura Zaki, Sami Dridi, Robert F. Wideman, Marco A. Rebollo, and Douglas D. Rhoads. Chondronecrosis with osteomyelitis in broilers: further defining lameness-inducing models with wire or litter flooring to evaluate protection with organic trace minerals. Poultry Science 99, 5422-5429, 2020.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
10. Ferver A, Dridi S. Phytogenics as an Alternative to Antibiotics: Chemical Mechanism behind Antimicrobial Activity of Essential Oils. Adv Food Technol Nutr Sci Open J. 2018; 4(2): e1-e3. Doi: 10.17140/AFTNSOJ-4-e012.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Greene E, Flees J, Dhamad A, Alrubaye A, Hennigan S, Pleimann J, Smeltzer M, Murray S, Kugel J, Goodrich J,
Robertson A, Wideman R, Rhoads D, Dridi S. Double-Stranded RNA Is a Novel Molecular Target in Osteomyelitis
Pathogenesis: A Translational Avian Model for Human Bacterial Chondronecrosis with Osteomyelitis. Am J Pathol. 2019
Oct;189(10):2077-2089. doi: 10.1016/j.ajpath.2019.06.013. Epub 2019 Aug 2
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
7. Lassiter K, Kong BC, Piekarski-Welsher A, Dridi S, Bottje W. Gene expression essential for myostatin signaling and skeletal muscle development is associated with divergent feed efficiency in pedigree male broilers. Front Physiol. 2019 Feb 26;10:126. doi: 10.3389/fphys.2019.00126. eCollection 2019
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Progress 10/01/18 to 09/30/19
Outputs
Target Audience:Using various sources (publications, classes, workshops, symposium and conferences), I was able to reach a wide audience (scientific communities, students, faculty, industry and producers) and hope that will have a large geographic influence and impact. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Training activity: mentoring 2 PhD students and trained one program associate Professional development: participation in workshops How have the results been disseminated to communities of interest?The results have been published in American Journal of Pathology which has an impact factor of 4.069. This journal is published by Elsevier on behalf of the American Society for Investigative Pathology. What do you plan to do during the next reporting period to accomplish the goals?We are planning in the future: 1) to determine the identity of dsRNA, and 2) identify potential other mechanisms and mediators
Impacts
What was accomplished under these goals?
During this period of the project, we made a breakthrough by identifying a molecular mechanism by which Staphylococcus induce bone degeneration in BCO chickens. Specifically, we found that bacterial infection reduces Dicer 1 expression and leads to the accumulation of double-stranded RNA which in turn activates NLRP3 inflammasome and increases pro-inflammatory cytokine production leading to bone cell death.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Greene E, Flees J, Dhamad A, Alrubaye A, Hennigan S, Pleimann J, Smeltzer M, Murray S, Kugel J, Goodrich J, Robertson A, Wideman R, Rhoads D, Dridi S. Double-Stranded RNA Is a Novel Molecular Target in Osteomyelitis Pathogenesis: A Translational Avian Model for Human Bacterial Chondronecrosis with Osteomyelitis. Am J Pathol. 2019 Oct;189(10):2077-2089. doi: 10.1016/j.ajpath.2019.06.013. Epub 2019 Aug 2
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Progress 10/01/17 to 09/30/18
Outputs
Target Audience:My efforts using various sources (publications, classes, workshops, symposium and conferences) reach a wide audience (students, faculty, industry and producers) and hope that will have a large geographic influence and impact. Changes/Problems:We developed an ELISA technique to measure dsRNA in circulation and in tissues. What opportunities for training and professional development has the project provided?I am training and mentoring during this project one Ph.D., one M.S., one undergraduate (Honors)student and one program associate. I also established and animate a weekly lab meeting that gather PIs and students to discuss data, projects and manuscripts. How have the results been disseminated to communities of interest?Our results have been disseminated to a wide audience (scientific communities, students, producers, industry,..) via publications in peer-reviewed journals, presentation in national and international meetings and conferences, symposium, classes as a guest lecturer and will be disseminated on my personal website. What do you plan to do during the next reporting period to accomplish the goals?I will continue and pursue my research to dissect the molecular mechanisms by which Staphylococcus induce bone attrition in BCO chickens. These insights may help to identify nutritional or managerial strategies to reduce the incidense of lamness in poultry. My plan is also to increase students recruitment and retention for advanced degrees and to create a high productive environment for learning and improving the research-teaching-reading nexus.
Impacts
What was accomplished under these goals?
During this period, we made a breakthrough by identifying that Staphylococcus infection dysregulates DICER1 expression (at mRNA and protein levels) and induces endogenous double-stranded RNA accumulation which in turn activates NLRP3 inflammasome. NLRP3 inflammasome is a protein complex that, when activated, leads to maturation of caspase 1 and production of pro-inflammtory cytokines (IL-18 and IL-1b) production. The activation of this pathways induces bone degeneraion via pyroptosis and apoptosis.
Publications
- Type:
Journal Articles
Status:
Submitted
Year Published:
2019
Citation:
Elizabeth Greene, Joshua Flees, Ahmed Dhamad, Adnan Alrubaye, Stephen Hennigan, Jason Pleimann, Sue Murray, Jennifer Kugel, James Goodrich, Avril Robertson, Robert Wideman, Douglas Rhoads, Sami Dridi*. Double-stranded RNA is a molecular target in osteomyelitis pathogenesis. Submitted to Biochem J
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Progress 10/01/16 to 09/30/17
Outputs
Target Audience:My efforts using various sources (publications, classes, workshops, symposium and conferences) reach a wide audience (students, faculty, industry and producers) and hope that will have a large geographic influence and impact. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?I am training and mentoring during this project one program associate, one visting scientistand oneundergraduate student (Honors students). I also established and animate a weekly lab meeting that gather PIs and students to discuss data, projects and manuscript How have the results been disseminated to communities of interest?Two manuscripts are under preparation. These manuscripts will be submitted to two peer-reviewed journals. What do you plan to do during the next reporting period to accomplish the goals?I will continue and pursuit my research to dissect the molecular mechanisms employed by Staphylococcus to induce bone attrition in both chickens and human.
Impacts
What was accomplished under these goals?
Aim 1: We found that Staphylococcus up regulates TLR4 expression, dysregulates DICER1 and activates NLRP3 inflammasome. Aim 2: We found that NLRP3 activation leads to caspase 1 maturation which in turn induces the expression of pro-inflammatory cytokines (IL-1b and IL-18) and bone degeneration.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2017
Citation:
Sami Dridi. Mechanistic understanding of feed and water intake in poultry. Chapter 5 in Burleigh Dodds science publishing (Editor, Todd Applegate), 320 pages, 2017.
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Progress 01/14/16 to 09/30/16
Outputs
Target Audience:My efforts using various sources (publications, classes, workshops, symposium and conferences) reach a wide audience (students, faculty, industry and producers) and hope that will have a large geographic influence and impact. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?We will begin to perform mechanistic studies using osteoblast cell lines to dissect the mechanisms ncRNA induce bone attrition. We plan to hire students (undergraduate, graduate, and honors) to assist with the research. We hope to increase student recruitment and retention for advanced degrees and to create a high productive environment for learning in the research-teaching arena.
Impacts
What was accomplished under these goals?
Our preliminary results, using proteomics, genomics, and immunoprecipitation, indicate that non-coding RNA play a potential role in lameness in chickens. We are working on identification of these ncRNA sequences and conducting mechanistic studies to determine how they induce bone cell degeneration.
Publications
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