Progress 02/01/16 to 01/31/20
Outputs
Target Audience:-Target Audiences Microbiologists Immunologists Veterinarians Fish health researchers Catfish producers -Efforts Science-based knowledge has been delivered in laboratory teaching, fish health-related courses, and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? This project has provided training and professional development opportunities for one undergraduate student, one graduate student, and one post-doctoral scientist. How have the results been disseminated to communities of interest? Three peer-reviewed journal articles were published. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
-Goal one: Construct T6SS and USP mutants. Nothing to report. -Goal two: Characterize T6SS and USP mutants Phagocytic properties of macrophages against selected mutants were determined Bactericidal properties of macrophages against selected mutants were determined Apoptotic changes in macrophages exposed to selected mutants was conducted Innate immune responses against selected mutants were determined by real-time qPCR Adaptive immune responses against selected mutants were determined by real-time qPCR IgM titers in catfish sera exposed to selected mutants were determined. 2) Specific objectives met -Goal one: Construct T6SS and USP mutants. Nothing to report. -Goal two: Characterize T6SS and USP mutants Innate and adaptive immune responses against selected mutants were determined. 3) Significant Results Achieved Safe and efficacious mutants were determined Vaccination increased the readiness of innate immunity Vaccination decreased wild-type replication in catfish Vaccination decreased the pathological lesions Vaccination induced protective immune responses Vaccination reduced inflammation caused by wild-type E. ictaluri Anterior kidney macrophages engulfed mutants more efficiently Anterior kidney macrophages destroyed internalized mutants more efficiently. Percentage of early and late apoptotic macrophages was increased after exposure to wild-type bacteria opsonized with serum from fish exposed to the wild-type strain. Gene expression analyses indicated that mutants could activate adaptive immune responses without causing inflammation. IgM titers indicated that mutants promote the development of primary immune responses and memory B cell formation. ?4) Key outcomes or other accomplishments realized Safe and efficacious mutant strains were identified Attenuated mutants increased the effectiveness of innate and adaptive immunity
Publications
- Type:
Journal Articles
Status:
Other
Year Published:
2020
Citation:
Ibrahim I, Abdelhamed H, Baumgartner W, Wills RW, Awadin W, Elbegawy M, Lawrence ML, Karsi A. Pathological and ultrastructural characterization of an Edwardsiella ictaluri hemR mutant (Ei?hemR?frdA?sdhC). In preparation.
- Type:
Journal Articles
Status:
Other
Year Published:
2020
Citation:
Kalindamar S, Abdelhamed H, Kordon A, Pinchuk L, Karsi A. The effect of Hcp family proteins of type VI secretion system in Edwardsiella ictaluri virulence. In preparation.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Kordon A, Kalindamar S, Majors K, Abdelhamed H, Tan W, Karsi A, Pinchuk L. 2020. Live attenuated Edwardsiella ictaluri vaccines enhance the protective innate immune responses of channel catfish B cells. Developmental and Comparative Immunology. 2020 Aug;109:103711. doi: 10.1016/j.dci.2020.103711. Epub 2020 Apr 18. PMID: 32311387.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Kalindamar S, Kordon A, Abdelhamed H, Tan W, Pinchuk L, Karsi A. 2019. Edwardsiella ictaluri evpP is required for colonization of channel catfish ovary cells and necrosis in anterior kidney macrophages. Cellular Microbiology. 2019 Nov 19:e13135. doi: 10.1111/cmi.13135. [Epub ahead of print]. PMID: 31742869.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Kordon A, Kalindamar S, Majors K, Abdelhamed H, Tan W, Karsi A, Pinchuk L. 2019. Effects of live attenuated vaccine and wild type strains of Edwardsiella ictaluri on phagocytosis, bacterial killing, and survival of catfish B cells. Frontiers in Immunology. 2019 Oct 9;10:2383. doi: 10.3389/fimmu.2019.02383. eCollection 2019. PMID: 31649682.
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Progress 02/01/18 to 01/31/19
Outputs
Target Audience:-Target Audiences Microbiologists Veterinarians Fish health researchers Catfish producers -Efforts Science-based knowledge has been delivered in laboratory teaching, fish health-related courses, and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? This project has provided training and professional development opportunities for two doctoral students (Adef Kordon, and Iman Ibrahim). Adef Kordon accepted a post-doctoral position in the College of Veterinary Medicine at Mississippi State University. Iman Ibrahim accepted a post-doctoral position in the Department of Microbiology, Immunology, and Biochemistry at The University of Tennessee Health Science Center. How have the results been disseminated to communities of interest?· Four peer-reviewed journal articles were published. · One peer-reviewed journal article was submitted. · Two dissertations were completed. · Six presentations were given at scientific conferences.? What do you plan to do during the next reporting period to accomplish the goals?Goal one: Construct T6SS and USP mutants. · None. This goal was completed in 2017. -Goal two: Characterize T6SS and USP mutants · Complete assessment of vaccine-induced innate and adaptive responses.
Impacts
What was accomplished under these goals?
1) Major activities completed -Goal one: Construct T6SS and USP mutants · This goal was completed in 2017. -Goal two: Characterize T6SS and USP mutants · Tissue persistence of selected mutants in juvenile catfish was determined · Pathologic examination of catfish exposed to selected mutants was completed · Phagocytic properties of macrophages against selected mutants were determined · Bactericidal properties of macrophages against selected mutants were determined · Phagocytic properties of B cells against selected mutants were determined · Bactericidal properties of B cells against selected mutants were determined · Apoptosis in macrophages exposed to selected mutants was conducted · Apoptosis in B cells exposed to selected mutants was conducted · Innate immune responses against selected mutants were determined by real-time qPCR · Adaptive immune responses against selected mutants were determined by real-time qPCR · IgM titers in catfish exposed to selected mutants were determined. 2) Specific objectives met -Goal one: Construct T6SS and USP mutants. · This goal was completed in 2017. -Goal two: Characterize T6SS and USP mutants · Innate and adaptive immune responses against selected mutants were determined. 3) Significant Results Achieved · Safe and efficacious mutant were determined · Vaccination increased the readiness of innate immunity · Vaccination decreased wild-type replication in catfish · Vaccination decreased the pathological lesions · Vaccination induced immune responses · Vaccination reduced damage caused by wild-type E. ictaluri · Peritoneal macrophages engulfed mutants more efficiently · Peritoneal macrophages destroyed internalized mutants more efficiently. · B cells engulfed mutant and wild-type bacteria similarly · B cells engulfed opsonized wild-type bacteria with sera from vaccinated fish efficiently · B cells killed opsonized wild-type bacteria with sera from vaccinated fish efficiently · The number of B cells decreased significantly after exposure to opsonized wild-type bacteria with serum from fish exposed to the wild-type strain · Percentage of early and late apoptotic cells were increased after exposure to wild-type bacteria opsonized with serum from fish exposed to the wild-type strain. · Gene expression analyses indicated that mutants can activate adaptive immune responses without causing inflammation. · IgM titers indicated that mutants promote the development of primary immune responses and memory B cell formation. 4) Key outcomes or other accomplishments realized · Highly safe and efficacious mutant strains were identified · Attenuated mutants increased the effectiveness of innate and adaptive immunity
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Kordon A, Abdelhamed H, Ahmed H, Baumgartner W, Karsi A, Pinchuk L. 2019. Assessment of the live attenuated and wild-type Edwardsiella ictaluri induced immune gene expression and Langerhans-like cell profiles in the immune-related organs of catfish. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.00392.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Akgul A, Nho S, Kalindamar S, Tekedar HC, Abdelhamed H, Lawrence ML, Karsi A. 2018. Universal stress proteins contribute Edwardsiella ictaluri virulence in catfish. Frontiers in Microbiology. 2018 Nov 28;9:2931. doi: 10.3389/fmicb.2018.02931. eCollection 2018. PMID: 30546354.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Abdelhamed H, Lawrence ML, Karsi A. 2018. Development and characterization of a novel live attenuated vaccine against enteric septicemia of catfish. Frontiers in Microbiology. 2018 Aug 7;9:1819. doi: 10.3389/fmicb.2018.01819. eCollection 2018. PMID: 30131791.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Akgul A, Akgul A, Lawrence ML, Karsi A. 2018. Stress-related genes promote Edwardsiella ictaluri pathogenesis. PLoS One. 2018 Mar 19;13(3):e0194669. doi: 10.1371/journal.pone.0194669. eCollection 2018. PMID: 29554143.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2018
Citation:
Kordon A, Kalindamar S, Majors K, Abdelhamed H, Tan W, Karsi A, Pinchuk L. 2018. Effects of live attenuated vaccine and wild type strains of Edwardsiella ictaluri on phagocytosis, bacterial killing, and survival of catfish B cells. Frontiers in Immunology.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Kalindamar S, Kordon A, Abdelhamed H, Pinchuk L, Lawrence ML, Karsi A. 2018. T6SS effector protein EvpP is essential for Edwardsiella ictaluri virulence in catfish. 8th International Symposium on Aquatic Animal Health. Charlottetown, Prince Edwards Island, Canada.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Majors K, Kordon A, Pray H, Pinchuk L, Karsi A. 2018. Role of catfish B-cells in innate and adaptive immune responses to Edwardsiella ictaluri live attenuated vaccines. Boehringer Ingelheim-NIH Veterinary Scholars Symposium. College Station, TX, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Kordon A, Kalindamar S, Abdelhamed H, Karsi A, Pinchuk L. 2018. Phagocytic and bactericidal properties of B cells in channel catfish exposed to Edwardsiella ictaluri live attenuated vaccine and wild-type strains. MCBIOS XV Conference. Starkville, MS, USA.
- Type:
Theses/Dissertations
Status:
Submitted
Year Published:
2018
Citation:
Kordon A. Antigen presenting cells-mediated innate and adaptive immune responses to live attenuated Edwardsiella ictaluri vaccines in channel catfish. Mississippi State University.
- Type:
Theses/Dissertations
Status:
Submitted
Year Published:
2018
Citation:
Ibrahim I. Assessment of live attenuated vaccines against enteric septicemia of channel catfish. Mississippi State University.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Karsi A, Lawrence ML, Pinchuk L, Petrie-Hanson L, Baumgartner W. 2018. Prevention of enteric septicemia of catfish by novel live attenuated vaccines. 2018 CRWAD Meeting. Chicago, IL, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Kordon A, Kalindamar S, Abdelhamed H, Tan W, Pinchuk L, Karsi A. 2018. Live attenuated Edwardsiella ictaluri vaccines stimulate active phagocytosis and bacterial killing of catfish b cells. 8th International Symposium on Aquatic Animal Health. Charlottetown, Prince Edwards Island, Canada.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Ibrahim I, Abdelhamed H, Baumgartner W, Lawrence ML, Karsi A. 2018. Pathological and immunological assessment of live attenuated vaccines against enteric septicemia of channel catfish. 8th International Symposium on Aquatic Animal Health. Charlottetown, Prince Edwards Island, Canada.
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Progress 02/01/17 to 01/31/18
Outputs
Target Audience:Target Audiences Microbiologists Veterinarians Fish health research community Catfish producers Efforts Science-based knowledge has been delivered in laboratory teaching, fish health-related courses, and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?- This project has provided training and professional development opportunities for two postdocs (Hossam Abdelhamed and Jingjun Lu) and four doctoral students (Ali Akgul, Safak Kalindamar, Adef Kordon, and Iman Ibrahim). - Hossam Abdelhamed accepted a faculty position in the College of Veterinary Medicine at Mississippi State University. - Jingjun Lu accepted a position in Saint Jude Children's Hospital. - Ali Akgul accepted a post-doctoral position in the College of Veterinary Medicine at the University of Florida. - Safak Kalindamar accepted a faculty position in a university in Turkey. How have the results been disseminated to communities of interest?- Five presentations were given at scientific conferences. - One manuscript was published in a peer-reviewed journal, Frontiers in Microbiology (https://doi.org/10.3389/fmicb.2017.02638). - One manuscript was submitted to peer-reviewed journals, PLOS One. What do you plan to do during the next reporting period to accomplish the goals?Goal one: Construct T6SS and USP mutants. - None. This goal is completed. Goal two: Characterize T6SS and USP mutants - Verify safety and efficacy of promising mutants in catfish fry - Assess vaccine-induced innate and adaptive responses.
Impacts
What was accomplished under these goals?
1) Major activities completed Goal one: Construct T6SS and USP mutants.? - All T6SS and USP mutants were constructed. Goal two: Characterize T6SS and USP mutants. - Attenuation of mutants was determined in catfish fry - Efficacy of mutants was determined in catfish fry. 2) Specific objectives met Goal one: Construct T6SS and USP mutants. - Construction of all T6SS and USP mutants were completed. Goal two: Characterize T6SS and USP mutants - Initial screening of mutants' virulence and efficacy in catfish fry was completed. 3) Significant Results Achieved Initial characterization indicated the presence of attenuated and protective mutants in catfish fry. 4) Key outcomes or other accomplishments realized Several USP and T6SS genes contribute to E. ictaluri virulence in catfish Phagocytosis of attenuated mutants by macrophages was higher compared to wild-type. Uptake of E. ictaluri opsonized with sera from vaccinated catfish was more efficient.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Kordon A, Abdelhamed H, Ahmed H, Park J, Karsi A, Pinchuk L. 2017. Phagocytic and bactericidal properties of channel catfish peritoneal macrophages exposed to Edwardsiella ictaluri live attenuated vaccine and wild-type strains. Frontiers in Microbiology (https://doi.org/10.3389/fmicb.2017.02638).
- Type:
Theses/Dissertations
Status:
Submitted
Year Published:
2017
Citation:
Akgul A. The role of universal stress proteins in Edwardsiella ictaluri virulence. Mississippi State University.
- Type:
Theses/Dissertations
Status:
Submitted
Year Published:
2017
Citation:
Kalindamar S. Characterization of type VI secretion system in Edwardsiella ictaluri. Mississippi State University.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Kalindamar S, Abdelhamed H, Kordon A, Lu J, Kumru S, Tekedar H, Pinchuk L, Karsi A. 2017. Characterization of type VI secretion system in Edwardsiella ictaluri. American Society for Microbiology South Central Branch Meeting, Little Rock, AR, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ibrahim I, Abdelhamed H, Baumgartner W, Lawrence M, Karsi A. 2017. Assessment of portals of entry for attenuated and wild-type Edwardsiella ictaluri. American Fisheries Society Fish Health Section Annual Meeting, East Lansing, MI, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ibrahim I, Abdelhamed H, Baumgartner W, Awadin W, Lawrence M, Karsi A. 2017. Pathological and ultrastructural effects of live attenuated Edwardsiella ictaluri vaccines. American Fisheries Society Fish Health Section Annual Meeting, East Lansing, MI, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Abdelhamed H, Lawrence ML, Karsi A. 2017. A novel vaccine candidate protects catfish against enteric septicemia. American Fisheries Society Fish Health Section Annual Meeting, East Lansing, MI, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Kalindamar S, Abdelhamed H, Kordon A, Al-Janabi N, Lu J, Tekedar H, Karsi A. 2017. The effect of Hcp family proteins of type VI secretion system in Edwardsiella ictaluri virulence. American Society for Microbiology ASM Microbe 2017 Meeting, New Orleans, LA, USA.
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Progress 02/01/16 to 01/31/17
Outputs
Target Audience:The target audiences include: Fish health research community Veterinarians Catfish producers Microbiologists Efforts Science-based knowledge has been delivered in laboratory teaching, fish health related courses, and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training and professional development opportunities for two postdocs (Hossam Abdelhamed and Jingjun Lu), three doctoral students (Ali Akgul, Safak Kalindamar, and Adef Kordon), and a visiting scholar (Iman Ibrahim). How have the results been disseminated to communities of interest?Results have been presented at scientific conferences and will be published in peer-reviewed journals. What do you plan to do during the next reporting period to accomplish the goals? Finalize construction of all T6SS and USP mutants. Determine safety and efficacy of mutants in catfish fingerlings. Confirm safety and efficacy of potential vaccine candidates in catfish fry. Start testing tissue persistence and immune responses of vaccine candidates.
Impacts
What was accomplished under these goals?
1) Major activities completed -Goal one: Construct T6SS and USP mutants Construction of T6SS mutants was completed. Construction of USP mutants was completed. -Goal two: Characterize T6SS and USP mutants Attenuation of mutants was determined in catfish fingerlings. Efficacy of mutants was determined in catfish fingerlings. 2) Specific objectives met -Goal one Construction of T6SS and USP mutants was completed. -Goal two Initial screening of mutants' virulence and efficacy was completed. 3) Significant Results Achieved We were able to construct all in-frame deletion mutants. Initial characterization indicated the presence of attenuated and protective mutants. 4) Key outcomes or other accomplishments realized Several USP and T6SS genes contribute to E. ictaluri virulence in catfish.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
Akgul A, Akgul A, Lawrence ML, Karsi A. 2016. Expression of Edwardsiella ictaluri stress-related genes under various stresses. American Society for Microbiology South Central Branch Meeting, Lafayette, LA, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
Kalindamar S, Abdelhamed H, Tekedar H, Karsi A. 2016. Construction and evaluation of an Edwardsiella ictaluri virulence protein F mutant. 2nd International Conference of Fish and Shellfish Immunology. Portland, MA, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
Akgul A, Akgul A, Tekedar H, Lawrence M, Karsi A. 2016. Expression analysis of universal stress proteins in Edwardsiella ictaluri. American Society for Microbiology ASM Microbe 2016 Meeting, Boston, MA, USA.
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