Progress 12/15/15 to 12/14/18
Outputs
Target Audience:Concepts and Results derived from our studies were used to illustrate classroom lectures in BIO 1113 (Biological Sciences: Energy Transfer and Development). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project provided the following opportunities for training and professional development in the Alonso lab at The Ohio State University: 1) the PD uses this project to illustrate the ongoing research in plant lipids during her classes offered on Spring 2017. Total 489 local participants, undergraduates; 2) Enkhtuul Tsogtbaatar, graduate student in Columbus (OH), completed her Ph.D. thesis in July 2017. The project provided the following opportunities for training and professional development in the Bates lab at the University of Southern Mississippi: 1) Graduate Student Sajina Bhandari presented her results at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism in Galveston, TX in February of 2017. 2) Undergraduate Nischal Karki graduated and presented his research at the Departmental Undergraduate Research Symposium. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest by the Alonso lab at The Ohio State University through publication, seminar and poster presentation: 1) Jean-Christophe Cocuron, Enkhtuul Tsogtbaatar, & Ana Paula Alonso published "High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry" in the Journal of Chromatography A (2017); 2) Ana Paula Alonso, seminar presenter, "Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops" presented at the US Department of Energy PI meeting. Arlington, VA (February 2017); 3) Ana Paula Alonso, invited speaker, "Modeling and analysis of plant metabolic networks to build the crops of tomorrow" presented at BioDiscovery Institute. Denton, TX (February 2017); 4) Ana Paula Alonso, invited speaker, "Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops" presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017); 5) Jean-Christophe Cocuron, poster presenter, "Tracking the flow of carbon toward oil synthesis on an emerging crop Lesquerella (Physaria fendleri)" presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017); 6) Jean-Christophe Cocuron, poster presenter, "High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry" presented at the Ohio Metabolomics Consortium. Columbus, OH (May 2017); 7) Ana Paula Alonso, seminar presenter, "A systems approach to improve oil synthesis in alternative crops" presented at the 7th International Congress on Biofuels and Bioenergy. Toronto, Canada (October 2017); 8) Ana Paula Alonso, invited speaker, "A systems approach to improve oil synthesis in alternative crops" presented at the Department of Horticulture and Crop Sciences Seminar series. Columbus, OH (October 2017). The results have been disseminated to communities of interest by the Bates lab at the University of Southern Mississippi: 1) Sajina Bhandari, poster presenter, "Does Lesquerella Mimic Arabidopsis or Castor, in Terms of TAG Assembly?" at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017); 2) Philip Bates, seminar presenter, "Investigations into the Control of Unusual Fatty Acid Induced Down Regulation of Acetyl-CoA Carboxylase by PII" at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017); 3) Philip Bates, Plenary Speaker, "Encouraging Obesity in Plants for a Cleaner, Healthier, and More Sustainable Future" at the Mississippi Academy of Sciences 81st Annual Meeting. in Hattiesburg, MS (Feb. 2017); 4) Philip Bates, seminar presenter, "Insights on the Eukaryotic Pathway of Leaf Lipid Synthesis from Acyl Flux Analysis within the Arabidopsis act1/lpcat1/lpcat2 Triple Mutant" at the 8th European Symposium on Plant Lipids in Malmo, Sweden (July 2017); 5) Philip Bates, seminar presenter, "Deciphering the "Eukaryotic Pathway" of Leaf Glycerolipid Assembly through Lipid Flux Analysis in Arabidopsis Mutants and Oil Accumulating Tobacco" at the 56th Annual Meeting of the Phytochemical Society of North America in Columbia, MO (Aug. 2017); 6) Philip Bates, invited speaker, "Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux?" at the Donald Danforth Plant Science Center. St. Louis, MO (Aug. 2017); 7) Philip Bates, invited speaker, "Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux?" at the Department of Chemistry Seminar Series, Jackson State University, Jackson, MS (Nov. 2017). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The following tasks were accomplished in the Alonso lab at The Ohio State University to accomplish the project goals: 1) validation and publication of a high-throughput method to quantify the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry; 2) the analysis of 13C-labeling in all the intermediary metabolites and biomass components in Physaria was performed by mass spectrometry, and the mass isotopomer distribution was determined for each compound under investigation; 3) determination of the optimal concentration for two inhibitors of fatty acid elongation in developing Physaria embryos; 4) mentoring one graduate student (Enkthuul Tsogtbaatar); and The following tasks were accomplished in the Bates lab at the University of Southern Mississippi to accomplish the project goals: 1) [14C]acetate labeling of fatty acid synthesis in developing Physaria embryos indicated newly synthesized fatty acids are first incorporated into the membrane lipid phosphatidylcholine prior to synthesis of lesquerolic acid and seed oils. 2) [14C]glycerol labeling unexpectedly indicated that Physaria utilizes a PC-derived DAG pathway of oil synthesis rather than the expected Kennedy pathway used by castor which also accumulates hydroxylated fatty acids. This result will lead to a new model of unusual fatty acid oil synthesis in Physaria. 3) Mentoring of one graduate student (Sajina Bhandari).
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Cocuron J-C, Tsogtbaatar E, Alonso A.P. (2017) High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry. J Chromatogr A. 1490, 148-155.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, seminar presenter, Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops presented at the US Department of Energy PI meeting. Arlington, VA (February 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, invited speaker, Modeling and analysis of plant metabolic networks to build the crops of tomorrow presented at BioDiscovery Institute. Denton, TX (February 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, invited speaker, Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Jean-Christophe Cocuron, poster presenter, Tracking the flow of carbon toward oil synthesis on an emerging crop Lesquerella (Physaria fendleri) presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Jean-Christophe Cocuron, poster presenter, High-throughput quantification of the levels and labeling abundance of free
amino acids by liquid chromatography tandem mass spectrometry presented at the Ohio Metabolomics Consortium. Columbus, OH (May 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, seminar presenter, A systems approach to improve oil synthesis in alternative crops presented at the 7th International Congress on Biofuels and Bioenergy. Toronto, Canada (October 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, invited speaker, A systems approach to improve oil synthesis in alternative crops presented at the Department of Horticulture and Crop Sciences Seminar series. Columbus, OH (October 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Sajina Bhandari, poster presenter, Does Lesquerella Mimic Arabidopsis or Castor, in Terms of TAG Assembly? at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, seminar presenter, Investigations into the Control of Unusual Fatty Acid Induced Down Regulation of Acetyl-
CoA Carboxylase by PII at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
ilip Bates, Plenary Speaker, Encouraging Obesity in Plants for a Cleaner, Healthier, and More Sustainable Future at the Mississippi Academy of Sciences 81st Annual Meeting. in Hattiesburg, MS (Feb. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, seminar presenter, Insights on the Eukaryotic Pathway of Leaf Lipid Synthesis from Acyl Flux Analysis within the Arabidopsis act1/lpcat1/lpcat2 Triple Mutant at the 8th European Symposium on Plant Lipids in Malmo, Sweden (July 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, seminar presenter, Deciphering the Eukaryotic Pathway of Leaf Glycerolipid Assembly through Lipid Flux
Analysis in Arabidopsis Mutants and Oil Accumulating Tobacco at the 56th Annual Meeting of the Phytochemical Society of North America in Columbia, MO (Aug. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, invited speaker, Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux? at the Donald Danforth Plant Science Center. St. Louis, MO (Aug. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, invited speaker, Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux? at the Department of Chemistry Seminar Series, Jackson State University, Jackson, MS (Nov. 2017)
|
Progress 12/15/16 to 12/14/17
Outputs
Target Audience:Concepts and results derived from our studies were used to illustrate classroom lectures in BIO 1113 (Biological Sciences: Energy Transfer and Development). Changes/Problems:A major change for the next reporting period: PD Ana Paula Alonso has accepted to join the University of North Texas as an Associate Professor and to become the Director of their BioAnalytical Facility, starting January 15th, 2018. The move is anticipated to delay some aspects of this project because The Ohio State University will freeze and delay the transfer of funds for at least 6 months. Therefore, a no-cost extension of a year will certainly be requested in 2018. However, the new environment that includes the BioDiscovery Institute (BDI), the significant expertise in oil metabolism, and the outstanding metabolic profiling equipment at UNT will all provide exceptional opportunities for enhanced collaborations and productivity for this project. What opportunities for training and professional development has the project provided?The project provided the following opportunities for training and professional development in the Alonso lab at The Ohio State University: 1) the PD uses this project to illustrate the ongoing research in plant lipids during her classes offered on Spring 2017. Total 489 local participants, undergraduates; 2) Enkhtuul Tsogtbaatar, graduate student in Columbus (OH), completed her Ph.D. thesis in July 2017. The project provided the following opportunities for training and professional development in the Bates lab at the University of Southern Mississippi: 1) Graduate Student Sajina Bhandari presented her results at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism in Galveston, TX in February of 2017. 2) Undergraduate Nischal Karki graduated and presented his research at the Departmental Undergraduate Research Symposium. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest by the Alonso lab at The Ohio State University through publication, seminar and poster presentation: 1) Jean-Christophe Cocuron, Enkhtuul Tsogtbaatar, & Ana Paula Alonso published "High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry" in the Journal of Chromatography A (2017); 2) Ana Paula Alonso, seminar presenter, "Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops" presented at the US Department of Energy PI meeting. Arlington, VA (February 2017); 3) Ana Paula Alonso, invited speaker, "Modeling and analysis of plant metabolic networks to build the crops of tomorrow" presented at BioDiscovery Institute. Denton, TX (February 2017); 4) Ana Paula Alonso, invited speaker, "Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops" presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017); 5) Jean-Christophe Cocuron, poster presenter, "Tracking the flow of carbon toward oil synthesis on an emerging crop Lesquerella (Physaria fendleri)" presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017); 6) Jean-Christophe Cocuron, poster presenter, "High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry" presented at the Ohio Metabolomics Consortium. Columbus, OH (May 2017); 7) Ana Paula Alonso, seminar presenter, "A systems approach to improve oil synthesis in alternative crops" presented at the 7th International Congress on Biofuels and Bioenergy. Toronto, Canada (October 2017); 8) Ana Paula Alonso, invited speaker, "A systems approach to improve oil synthesis in alternative crops" presented at the Department of Horticulture and Crop Sciences Seminar series. Columbus, OH (October 2017). The results have been disseminated to communities of interest by the Bates lab at the University of Southern Mississippi: 1) Sajina Bhandari, poster presenter, "Does Lesquerella Mimic Arabidopsis or Castor, in Terms of TAG Assembly?" at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017); 2) Philip Bates, seminar presenter, "Investigations into the Control of Unusual Fatty Acid Induced Down Regulation of Acetyl-CoA Carboxylase by PII" at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017); 3) Philip Bates, Plenary Speaker, "Encouraging Obesity in Plants for a Cleaner, Healthier, and More Sustainable Future" at the Mississippi Academy of Sciences 81st Annual Meeting. in Hattiesburg, MS (Feb. 2017); 4) Philip Bates, seminar presenter, "Insights on the Eukaryotic Pathway of Leaf Lipid Synthesis from Acyl Flux Analysis within the Arabidopsis act1/lpcat1/lpcat2 Triple Mutant" at the 8th European Symposium on Plant Lipids in Malmo, Sweden (July 2017); 5) Philip Bates, seminar presenter, "Deciphering the "Eukaryotic Pathway" of Leaf Glycerolipid Assembly through Lipid Flux Analysis in Arabidopsis Mutants and Oil Accumulating Tobacco" at the 56th Annual Meeting of the Phytochemical Society of North America in Columbia, MO (Aug. 2017); 6) Philip Bates, invited speaker, "Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux?" at the Donald Danforth Plant Science Center. St. Louis, MO (Aug. 2017); 7) Philip Bates, invited speaker, "Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux?" at the Department of Chemistry Seminar Series, Jackson State University, Jackson, MS (Nov. 2017). What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, the Alonso lab is moving to The University of North Texas and anticipates to complete: 1) develop a mathematical modeling of 13C-labeling to build a map of C partitioning in Physaria embryos ; 2) test the addition of inhibitors in fatty acid synthesis and composition; 3) mentoring graduate and undergraduate students; and 4) the presentation of the results at conferences. During the next reporting period, the Bates lab at the University of Southern Mississippi anticipates to complete: 1) Perform [14C]glycerol pulse-chase results to investigate triacylglycerol remodeling; 2) collaborate to test inhibitors of fatty acid synthesis of on acyl lipid flux; 3) mentoring graduate and undergraduate students; and 4) the presentation of the results at conferences; 5) publish the [14C]acetate and [14C]glycerol labeling results.
Impacts
What was accomplished under these goals?
The following tasks were accomplished in the Alonso lab at The Ohio State University to accomplish the project goals: 1) validation and publication of a high-throughput method to quantify the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry; 2) the analysis of 13C-labeling in all the intermediary metabolites and biomass components in Physaria was performed by mass spectrometry, and the mass isotopomer distribution was determined for each compound under investigation; 3) determination of the optimal concentration for two inhibitors of fatty acid elongation in developing Physaria embryos; 4) mentoring one graduate student (Enkthuul Tsogtbaatar); and The following tasks were accomplished in the Bates lab at the University of Southern Mississippi to accomplish the project goals: 1) [14C]acetate labeling of fatty acid synthesis in developing Physaria embryos indicated newly synthesized fatty acids are first incorporated into the membrane lipid phosphatidylcholine prior to synthesis of lesquerolic acid and seed oils. 2) [14C]glycerol labeling unexpectedly indicated that Physaria utilizes a PC-derived DAG pathway of oil synthesis rather than the expected Kennedy pathway used by castor which also accumulates hydroxylated fatty acids. This result will lead to a new model of unusual fatty acid oil synthesis in Physaria. 3) Mentoring of one graduate student (Sajina Bhandari).
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Cocuron J-C, Tsogtbaatar E, Alonso A.P. (2017) High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry. J Chromatogr A. 1490, 148-155.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, seminar presenter, Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops presented at the US Department of Energy PI meeting. Arlington, VA (February 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, invited speaker, Modeling and analysis of plant metabolic networks to build the crops of tomorrow presented at BioDiscovery Institute. Denton, TX (February 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, invited speaker, Application of metabolomics and fluxomics to study fatty acid synthesis in alternative crops presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, seminar presenter, A systems approach to improve oil synthesis in alternative crops presented at the 7th International Congress on Biofuels and Bioenergy. Toronto, Canada (October 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Jean-Christophe Cocuron, poster presenter, Tracking the flow of carbon toward oil synthesis on an emerging crop Lesquerella (Physaria fendleri) presented at the American Society for Biochemistry and Molecular Biology. Chicago, IL (April 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Jean-Christophe Cocuron, poster presenter, High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry presented at the Ohio Metabolomics Consortium. Columbus, OH (May 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ana Paula Alonso, invited speaker, A systems approach to improve oil synthesis in alternative crops presented at the Department of Horticulture and Crop Sciences Seminar series. Columbus, OH (October 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Sajina Bhandari, poster presenter, Does Lesquerella Mimic Arabidopsis or Castor, in Terms of TAG Assembly? at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, seminar presenter, Investigations into the Control of Unusual Fatty Acid Induced Down Regulation of Acetyl-CoA Carboxylase by PII at the Gordon Research Conference: Plant Lipids Structure, Function & Metabolism, Galveston, TX (Feb. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, Plenary Speaker, Encouraging Obesity in Plants for a Cleaner, Healthier, and More Sustainable Future at the Mississippi Academy of Sciences 81st Annual Meeting. in Hattiesburg, MS (Feb. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, seminar presenter, Insights on the Eukaryotic Pathway of Leaf Lipid Synthesis from Acyl Flux Analysis within the Arabidopsis act1/lpcat1/lpcat2 Triple Mutant at the 8th European Symposium on Plant Lipids in Malmo, Sweden (July 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, seminar presenter, Deciphering the Eukaryotic Pathway of Leaf Glycerolipid Assembly through Lipid Flux Analysis in Arabidopsis Mutants and Oil Accumulating Tobacco at the 56th Annual Meeting of the Phytochemical Society of North America in Columbia, MO (Aug. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, invited speaker, Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux? at the Donald Danforth Plant Science Center. St. Louis, MO (Aug. 2017)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Philip Bates, invited speaker, Deciphering Bottlenecks within Plant Oil Engineering: What Controls Fatty Acid Flux? at the Department of Chemistry Seminar Series, Jackson State University, Jackson, MS (Nov. 2017)
|
Progress 12/15/15 to 12/14/16
Outputs
Target Audience:Concepts and results derived from our studies were used to illustrate classroom lectures in MolGen 5735 (Plant Biochemistry), MolGen 3436 (Introduction to Plant Physiology), ART 5001 (Aspects of Art and Technology), and the Metabolomics module of the Plant Functional Genomics Workshop. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project provided the following opportunities for training and professional development in the Alonso lab at The Ohio State University: 1) the PD was the lead instructor, Jean-Christophe Cocuron (Research Associate) and Enkhtuul Tsogtbaatar (graduate student) were co-instructors of the Metabolomics module of the Practical Summer Workshop in Functional Genomics offered on June 2016, Columbus, OH. Total 17 international and national participants, graduate students & postdocs; 2) the PD was instructor in the Minicourse "Use of Mass Spectrometry in Biological Systems" of the 9th Tripartite Meeting on November 2016, Maresias, Brazil. Total 30 international participants, graduate students, postdocs and faculty members; 3) the PD uses this project to illustrate the ongoing research in plant lipids during her classes offered on Spring and Fall 2016. Total 44 local participants, undergraduates & graduate students; 4) the PD and Jean-Christophe Cocuron provide guest lectures and tours of the Targeted Metabolic Laboratory to demonstrate the application of mass spectrometry to ongoing research. Total 105 participants, undergraduate students, middle schoolers and teachers; 5) Erin Ponting, undergraduate student in Columbus (OH), completed her Honor's thesis research project entitled "Towards the biosynthetic pathway of lesquerolic acid in Physaria fendleri" on April 2016; and 6) Siri Taxerås, Visiting Scholar from Norway in Columbus (OH), completed her bachelor research project entitled "Validating culture conditions for plant oil production in Physaria fendleri embryos" on June 2016. The project provided the following opportunities for training and professional development in the Bates lab at the University of Southern Mississippi: 1) This project has provided funding for the training of one female PhD graduate student, and partial funding of one undergraduate researcher at the University of Southern Mississippi; 2) The graduate student has also been accepted to attend a prestigious Gordon Conference on Plant Lipids during January of 2017. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest by the Alonso lab at The Ohio State University through seminar and poster presentation: 1) Jean-Christophe Cocuron, seminar presenter, "A systems approach to understanding and improving industrial oil biosynthesis in an emerging crop Physaria fendleri" presented at the Center for Applied Plant Sciences seminar series. Columbus, OH (April 2016); and 2) Erin Ponting, poster presenter, "Determination of the biosynthetis pathway of lesquerolic acid in Physaria fendleri through chemical inhibition" presented at the Denman Research Forum. Columbus, OH (March 2016). The results have been disseminated to communities of interest by the Bates lab at the University of Southern Mississippi: The undergraduate has presented his research at an undergraduate research forum at the University of Southern Mississippi. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, the Alonso lab at The Ohio State University anticipates to complete: 1) the analysis of 13C-labeling in all the intermediary metabolites and biomass components in Physaria by mass spectrometry; 2) the mentoring of graduate and undergraduate students; and 3) the presentation of the results at the American Society for Biochemistry and Molecular Biology (Chicago, April 2017). During the next reporting period, the Bates lab at the University of Southern Mississippi anticipates to complete: 1) the analysis of acyl editing cycle and DAG pool utilization for TAG synthesis; 2) the 14C-glycerol metabolic labeling of Physaria embryos; 3) the analysis of DAG flux for Kennedy pathway or PC derived DAG pathway of TAG synthesis; and 4) the presentation of the results at the Plant Lipid Gordon conference in Galveston, TX in January of 2017.
Impacts
What was accomplished under these goals?
The following tasks were accomplished in the Alonso lab at The Ohio State University to accomplish the project goals: 1) 13C-steady state labeling of developing Physaria embryos has been completed using several 13C-labeled substrates, and the efficiency with which Physaria embryos store carbon into biomass was determined; 2) mentoring two undergraduate students (Erin Ponting and Siri Taxerås), and one graduate student (Enkthuul Tsogtbaatar); and 3) Development of a high-throughput method to quantify the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry (publication under review). The following tasks were accomplished in the Bates lab at the University of Southern Mississippi to accomplish the project goals: 1) a hydroxylated triacylglycerol molecular species specific accumulation curve during P. fendleri seed development has been generated to identify tissue specific staging for analysis of lipid fluxes; 2) 14C-acetate metabolic labeling of Physaria embryos has been completed; and 3) the analysis of acyl editing cycle and DiAcylGlycerol (DAG) pool utilization for TriAcylGlycerol (TAG) synthesis is ahead of schedule and should be completed by early 2017. These initial results may suggest that the kinetics (and possibly pathway) of fatty acid provision for cytosolic elongation of 18 carbon fatty acids to 20 carbon fatty acids is different in P. fendleri than the related and previously characterized species Brassica rapa and Arabidopsis thaliana.
Publications
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2016
Citation:
Erin Ponting completed her Honor's thesis research project: Towards the biosynthetic pathway of lesquerolic acid in Physaria fendleri
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2016
Citation:
Siri Taxeras completed her Bachelor research project: "Validating culture conditions for plant oil production in Physaria fendleri embryos"
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
Jean-Christophe Cocuron, seminar presenter, A systems approach to understanding and improving industrial oil biosynthesis in an emerging crop Physaria fendleri presented at the Center for Applied Plant Sciences seminar series. Columbus, OH (April 2016)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
Erin Ponting, poster presenter, Determination of the biosynthetis pathway of lesquerolic acid in Physaria fendleri through chemical inhibition presented at the Denman Research Forum. Columbus, OH (March 2016)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
The undergraduate has presented his research at an undergraduate research forum at the University of Southern Mississippi.
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