Source: UNIV OF ARKANSAS submitted to
BUILDING TEACHING, RESEARCH AND EXTENSION CAPACITY AT UAPB TO ENHANCE GLOBAL LEARNING AND STRENGTHEN SWEET POTATO PRODUCTION IN GUYANA AND THE ARKANSAS DELTA
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1008474
Grant No.
2015-38821-24365
Project No.
ARX02015-05359
Proposal No.
2015-05359
Multistate No.
(N/A)
Program Code
EQ
Project Start Date
Sep 1, 2015
Project End Date
Aug 31, 2020
Grant Year
2015
Project Director
Manoharan, M.
Recipient Organization
UNIV OF ARKANSAS
(N/A)
PINE BLUFF,AR 71601
Performing Department
Agriculture
Non Technical Summary
The major goals ofthe project include: 1) training UAPB and Guyana students in research involving germplasm development through laboratory and field based experiences; 2) establishing a polycross and virus-testing laboratory in Guyana and building upon UAPB's Foundation Seed Program and germplasm development research; 2) developing insect resistant sweet potato lines through breeding and genomics; and 3) strengthening the extension program in sweet potato production to effectively meet the needs of farmers in the Arkansas Delta and Guyana.Project impacts include increase in insect and disease resistant germplasm available to sweet potato breeders and the possible reduction of insecticides/pesticides because of increased tolerance of new sweet potato genotypes, providing international research and work experience to UAPB and Guyana undergraduate and graduate students, and increased extension training and outreach. Moreover, increased number of small and limited-resource farmers will devote acreage to the high-value sweet potato crop, increasing the economic stability of their farming operation and adding value to rural livelihoods. UAPB students will see, first-hand, the global connectivity of agriculture. Guyana students and faculty will gain exposure to the role of biotechnology in research, teaching and extension activities
Animal Health Component
0%
Research Effort Categories
Basic
(N/A)
Applied
50%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2011450104050%
2021450108150%
Goals / Objectives
The major goals ofthe project include: 1) training UAPB and Guyana students in research involving germplasm development through laboratory and field based experiences; 2) establishing a polycross and virus-testing laboratory in Guyana and building upon UAPB's Foundation Seed Program and germplasm development research; 2) developing insect resistant sweet potato lines through breeding and genomics; and 3) strengthening the extension program in sweet potato production to effectively meet the needs of farmers in the Arkansas Delta and Guyana.
Project Methods
Objective 1: Developing Polycross & Virus-Indexing Laboratory in Guyana Overall strategy for objective: 1) To develop Polycross & Virus-indexing laboratory in Guyana; and 2) To train the faculty/scientists/students in breeding and virus-indexing. Experimental Procedure: 1. The Polycross and Virus-indexing laboratory will be established in Guyana- the laboratory will have the necessary equipment such as Laminar chamber, plant tissue culture chamber, PCR machine etc. 2. Shoot meristem will be aseptically isolated from Guyana sweet potato lines and cultured on MS medium with growth hormone. The regenerated plants will be grafted onto virus-sensitive Ipomea setosa plants. 3. DNA (and RNA) will be isolated from Ipomea setosa plants and tested for the presence/absence of viruses by PCR, and 4. The plants that are virus-free will be further multiplied for distribution to farmers, and 5. Scientist and students involved in this project will be trained in virus-indexing.Objective 2: Identifying the elite performing hybrid-parents through heterosis breeding in Guyana and UAPB Overall strategy for objective: 1) To choose the elite parental lines resistance to insects and diseases; 2) To develop hybrids in Line x Tester fashion; 3) To evaluate the hybrids in different locations in Guyana and UAPB; 4) To select the elExperimental Procedure: 1. Selecting the elite parents: The elite parents which are resistant to insects such as whiteflies (Bemisia tabaci) and Aphids (Aphis gossypii) from Guyana and USDA germplasm lines will be selected. The selected lines will be grafted to Ipomoea fistulosa to induce flowering in the controlled crossing blocks in Guyana and UAPB. 2. Crossing Blocks: The controlled crossed seeds will be harvested and germinated on the MS medium. 3. Evaluating the hybrids: The germinated seeds will be multiplied in the greenhouse. The selected lines will be evaluated for the resistance in the field. The General Combining Ability (GCA) and Specific Combining Ability (SCA) will be used to understand the heterosis of the parents and to select the elite lines. 4. Farmer-partcipatory approach: The selected hybrids will be evaluated under different locations in Arkansas and Guyana. The elite parents will be selected through the farmer-participatory approach by involving the farmers. Objective 3: Association mapping of EST-SSRs and SNPs in sweet potato (UAPB) Overall strategy for objective: Genome-Wide Association Study (GWAS) will be carried out using the functional polymorphic markers from comparative RNA-seq analysis developed at UAPB to understand the genomic regions involved inThe objectives are: 1) Designing primers for EST-SSRs and SNPs from the coding sequences from the transcriptome sequencing 2) Both heirloom varieties and USDA world germplasm lines including from Guyana will be identified and requested and multiplied 3) Genotyping efforts will be taken care of both EST-SSRs and SNPs. The resulting genotypic data will be compiled and preliminary analysis will be carried out. weevil resistance and yield-related traits in sweet potato. Experimental procedures: (a) Collection and multiplication of germplasm lines: From USDA-GRIN the appropriate heirloom varieties and mapping populations will be identified and requested. The requested lines will be multiplied and maintained for screening in the summer 2016, 2017 and 2018. DNA will be extracted from the established plants. (b) Designing primers for EST-SSRs and SNPs: The specific primers for EST-SSRs and SNPs will be designed and oligo-synthesis will be carried out. (c) Genotyping of EST-SSRs: The synthesized primers will be PCR amplified and separated using PAGE-Gels and scored. The genotyping scoring will be carried out. (d) Synthesis SNP slides with iScan illumina/KASPar assay: the designed SNPs will be sent to illumina sequencing facility or KASPar. Both EST-SSRs and SNPs data will be compiled for association mapping. Objective 4: Sustainable extension program in sweet potato production Overall strategy for objective: 1) To share the research results in a user-friendly manner with sweet potato producers in Guyana, Arkansas and, potentially, other parts of the southeastern region of the U.S. Experimental Procedure: A total of four outreach workshops to sweet potato producers in Guyana and the U.S- Two workshops will be conducted at UAPB and two workshops will be conducted in Guyana in conjunction with local partners. Demonstration activities will take place on producer farms and at association meetings of local agriculture producers.

Progress 09/01/15 to 08/31/20

Outputs
Target Audience:Sweetpotato farmers in Arkansas and Guyana Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained six scientists (for a month) from Guyana in sweetpotato virus-indexing and other molecular techniques. Scientists received training in techniques in meristem culture to develop virus testing laboratories at the University of Guyana (UG) and the National Agriculture Research and Extension Institute (NAREI). These scientists observed UAPB's Foundation Seed Program and breeding program. A total of 21 undergraduate students were involved in this project. Three graduate students were supported from this project. Seven students completed study abroad program in Guyana. The students were accompanied by mentors. Contacts with faculty and scientists in Guyana were strengthened and new contacts were made by the UAPB faculty as they implemented the project. How have the results been disseminated to communities of interest?The results were shared with the Arkansas Sweetpotato growers. Presentations were made at the Association of 1890 Research Directors Symposium, UAPB SAFHS field-day, and at the Annual Meeting of the East Arkansas Enterprise Community. Contacts were made with sweetpotato producers in Guyana. Presentations were also made at the Plant & Animal Genome meeting. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Sweetpotato breeding program was initiated in Guyana with the goal to select insect resistant lines. Virus-indexing and Polycross Laboratory has been established at the University of Guyana. Laboratory equipment were acquired. Two Poly-cross nurseries were established, and the seeds were obtained from selected genotypes. Twenty-five advanced breeding lines was evaluated. Sweetpotato seedling production and evaluation was done at two locations in Guyana. Three advanced field trials were conducted at the National Agriculture Research and Extension Institute (NAREI) with selections from seedlings produced in the NAREI poly-cross nursery. The University of Guyana also conducted an advanced trial at the NAREI location in MonRepos. The evaluations have resulted in the selection of five advanced lines that were evaluated at three select locations in Guyana. In addition, two lines were selected for entry into the poly-cross nursery. UAPB's scientist assisted both UG and NAREI with evaluations and the selection of advanced lines for insect resistance. At UAPB, a set of 139 heirloom accessions from 37 different countries were obtained from USDA. The slips were multiplied in the greenhouse. The multiplied slips were then planted in the field with three replications using randomized complete block design. Eighteen morphological and eleven storage root traits were evaluated. Transcriptome sequencing was carried out and SNPs were identified.

Publications


    Progress 09/01/17 to 08/31/18

    Outputs
    Target Audience:Sweetpotato Producers in Arkansas. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Nine University of Guyana (UG) students were selected for support of their senior projects and all nine students completed their projects. These students also assisted the UG collaborators with laboratory, shade-house and field sweet potato projects. This was the third cohort of UG students that participated in the project. Two scientist form Guyana came to the University of Arkansas for training in biotechnology and to observe UAPB's Foundation Seed Program and breeding program. This was a four-week visit supported by the project. Two students from Guyana completed their M.S. in Agriculture Regulations at UAPB How have the results been disseminated to communities of interest?The results were shared with the Arkansas Sweetpotato growers. What do you plan to do during the next reporting period to accomplish the goals?1. Complete equipment acquisition for the University of Guyana 2. Genotyping of insect-resist lines developed in Guyana

    Impacts
    What was accomplished under these goals? Sweet potato seedling production and evaluation continued at the two locations in Guyana. Three advanced field trials were conducted at the National Agriculture Research and Extension Institute (NAREI) with selections from seedlings produced in the NAREI poly-cross nursery. The University of Guyana also conducted an advanced trial at the NAREI location in Mon Repos. The evaluations have resulted in the selection of five advanced lines that will be evaluated at three select location in Guyana. In addition, two lines were selected for entry into the poly-cross nursery. UAPB's scientist assisted both UG and NAREI with evaluations and the selection of advanced lines.

    Publications


      Progress 09/01/16 to 08/31/17

      Outputs
      Target Audience:Sweetpotato Farmers Changes/Problems:Changes/Problems: None What opportunities for training and professional development has the project provided?2. What opportunities for training and professional development has the project provided? Four scientists from Guyana have come to the biotechnology laboratory and received training in techniques in meristem culture to develop virus testing laboratories at the University of Guyana (UG) and the National Agriculture Research and Extension Institute (NAREI). One University of Arkansas at Pine Bluff (UAPB) faulty member traveled to Guyana to supervise UAPB undergrad students completing international study as part of this project. This was this faculty member's first international experience. Contacts with faculty and scientists in Guyana were strengthened and new contacts were made by the UAPB faculty as they implemented the project. Five UAPB students completed study-aboard programs in Guyana. One of the graduate students from Guyana presented an oral paper at the Association of 1890 Research Directors Symposium and was awarded third place in the graduate student competition in Plant Science. How have the results been disseminated to communities of interest?3. How have the results been disseminated to communities of interest? One oral presentation was given at the Association of 1890 Research Directors Symposium. Presentations were given at the SAFHS field-day and at the Annual Meeting of the East Arkansas Enterprise Community. Contacts were made with sweetpotato producers in Guyana where the project was discussed and farmers were recruited to take part in future advanced trials when selected sweetpotato lines are ready for testing. What do you plan to do during the next reporting period to accomplish the goals?4. What do you plan to do during the next reporting period to accomplish the goals? We will continue to collect seed from the Poly-cross Nursery. Seedling evaluation will continue in an effort to develop new cultivars. We will evaluate our screening method in an attempt to strengthen the detection of insect resistant lines. The equipment for biotech laboratory at UG will be installed and brought into full operation. Additional students will be selected for senior project support at UG and student recruitment for UAPB will continue. Additional undergraduate students will be selected to participate in the study-aboard program. Association mapping of EST-SSRs and SNPs in sweet potato will be completed. We will report project developments as appropriate for research and Outreach findings.

      Impacts
      What was accomplished under these goals? 1. What Major accomplished under these goals? Renovation of the biotechnology infrastructure renovation was completed and is now ready for instillation of equipment. Two Poly-cross nurseries were established and are now producing seed form the selected genotypes installed in the nurseries. Screening of breeding lines was initiated and we now have approximately twenty-five advanced lines that are being evaluated. Nine University of Guyana (UG) students were supported with research grants to support senior projects. Three students from UG have been admitted to the Agricultural Regulation graduate program at the University of Arkansas at Pine Bluff. Two of these students are supported by this project. Transcriptome sequencing was carried out and SNPs identified.

      Publications


        Progress 09/01/15 to 08/31/16

        Outputs
        Target Audience:Sweetpotato farmers in Arkansas and Guyana Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained two scientists (for amonth) from Guyana in sweetpotato virus-indexing and other molecular techniques. Two students from UAPB traveled to Guyana in summer to learn about sweetpotato production practices. How have the results been disseminated to communities of interest?Presented results to sweetpotato farmers. What do you plan to do during the next reporting period to accomplish the goals?Complete SNP analysis and equipment accquisition for virus-indexing laboratory for the production of virus-free slips. Advance breeding program in Guyana to produce insect-resistant sweetpotatoes and provide training for students and scientists in sweetpotato indexing and production.

        Impacts
        What was accomplished under these goals? Graduate students are being trained in genome wide association studies. A set of 139 heirloom accessions from 37 different countries were obtained from USDA. The slips were multiplied in the greenhouse. The multiplied slips were then planted in the field with three replications using randomized complete block design. Eighteen morphological and eleven storage root traits were evaluated. Also, SNP marker analysis is in progress. We are also in the process establishing a polycross laboratory in Guyana. Laboratory renovation was completed and accquiring of equipment for the lab is in progress. Further, sweetpotato breeding program was initiated in Guyana with the goal to select insect resistant sweetpotato.

        Publications