Source: UNIVERSITY OF MISSOURI submitted to NRP
N-3 POLYUNSATURATED FATTY ACIDS AND HUMAN HEALTH AND DISEASE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1008465
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
NC-1199
Project Start Date
Nov 23, 2015
Project End Date
Sep 30, 2017
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF MISSOURI
(N/A)
COLUMBIA,MO 65211
Performing Department
Food Systems & Bioengineering
Non Technical Summary
This project will develop nanoemulsions for optimum delivery of functional omega-3 fatty acids. The nanoemulsions will be formed and stabilized using protein and polysaccharide. The use of natural ingredients, protein and polysaccharide, will respond to the need of the food industry in search of clean-labelled ingredients. The project aims to optimize the emulsion stability and delivery of omega-3. The outcome of the project will assist food manufacturer to design food and beverage with improved human health and wellness.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5015010101050%
5025010101050%
Goals / Objectives
To determine the effects of omega-3 fatty acids on health-related outcomes in both human and rodent models.
Project Methods
This project will develop protein and polysaccharide-based emulsifier and stabilizer. The advantage of these ingredients will be their ability to form and stabilize nanoemulsions at the pH near isoelectric pH of the protein. The effect of these ingredients on emulsion properties and stability as well as their digestion and delivery of n-3 fatty acids will be investigated. Initial experiments will be designed to provide a comprehensive understanding of the molecular basis of the ingredients that lead to formation of nanoemulsions with maximum stability. A series of techniques such as dynamic light scattering, zeta potential measurement and microscopy will be used to characterize the emulsion. The influence of factors affecting protein and polysaccharide interactions will be evaluated to identify optimum formulations leading to maximum stability. The fate of the emulsions during digestion will be determined using a simulated gastrointestinal tract model. The major factor affecting the digestion of the emulsion and the bioavailability of n-3 fatty acids will be determined. Finally, the optimum emulsion systems will be incorporated into model foods to establish their commercial potential. This project addresses the goals of the Experimental Station Committee on Organization and Policy Science Roadmap Challenge 5 - We must improve human health, nutrition, and wellness of the US population. This project also addresses Food and Nutrition Crosscutting Research Areas (NCRA) and Objectives of the North Central Regional Association: Research Objective 1 - Emphasize research that expands our understanding of the relationship between diet, health and disease prevention with particular focus on antioxidants, dietary lipids, functional foods/nutraceuticals, nutrient bioavailability, nutrient regulation of gene expression and nutrition and physical activity. The project will focus on optimizing the delivery and bioavailability of n-3 fatty acid in food products for maximum health benefits. Knowledge gained from this study will lead to development of optimum delivery systems for n-3 fatty acid which will allow the production of n-3 fatty acid enhanced food products for health and wellness.

Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Food industry and academia. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One graduate (MS) student has worked on the project. How have the results been disseminated to communities of interest?The results of this project were disseminated to community of interest through following ways: (i)publications of abstracts followed by oral or poster presentations at various international and national conferences (ii) delivering invited and keynote presentations at various workshops, symposia and Scientific and industry conferences (iii) discussions at various industry meetings. What do you plan to do during the next reporting period to accomplish the goals?The multi-state project has been terminated.

Impacts
What was accomplished under these goals? The projectinvestigated the effect of pectin on digestion properties and encapsulation of β-carotene of whey protein-stabilized emulsion. Unheated and heated whey protein-pectin mixtures (UH-Mix and H-Mix, respectively) and biopolymer ratios were studied.Results showed pectin led to a drastic increase in mean droplet sizes during digestion. At similar pectin concentration, the largest increase was from UH-Mix followed by H-Mix formed at 1, 3, and 5% protein. Overall, droplet sizes increased when pectin increased. Zeta potential results showed no significant differences among samples. Confocal images revealed that emulsions stabilized by unheated or heated WPI had the highest degree of coalescence. Extensive flocculation but less degree of coalescence was observed in emulsions containing pectin. Emulsions stabilized by H-Mix were composed of smaller and well-defined droplets compared. Coincided with the microscopy results, H-Mix systems also had the lowest β-carotene release at the end of digestion (e.g., 6.4% release compared to 17% release in systems without pectin). Furthermore, when the emulsions contained higher protein β-carotene release decreased.We concluded that heating, biopolymer concentration, and protein concentration in the emulsions played the major roles in stabilizing the emulsion and β-carotene during digestion. Results can be applied to improve delivery properties of WPI-stabilized emulsions.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Tang, Y., & Vardhanabhuti, B. (2017, June). Effect of pectin on digestion properties and ?-carotene delivery of whey protein-stabilized emulsions. Presented at the American Dairy Science Association Annual Meeting, Pittsburge, PA.


Progress 11/23/15 to 09/30/17

Outputs
Target Audience:Food industry and academia. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One graduate (MS) student has been trained on this project. How have the results been disseminated to communities of interest? The results of this project were disseminated to community of interest through following ways: (i) publications of abstracts followed by oral or poster presentations at various international and national conferences, (ii) delivering invited presentation and communicating with industry. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The project investigated the effect of pectin on digestion properties and encapsulation of β-carotene of whey protein-stabilized emulsion. Unheated and heated whey protein-pectin mixtures (UH-Mix and H-Mix, respectively) and biopolymer ratios were studied. Results showed pectin led to a drastic increase in mean droplet sizes during digestion. At similar pectin concentration, the largest increase was from UH-Mix followed by H-Mix formed at 1, 3, and 5% protein. Overall, droplet sizes increased when pectin increased. Zeta potential results showed no significant differences among samples. Confocal images revealed that emulsions stabilized by unheated or heated WPI had the highest degree of coalescence. Extensive flocculation but less degree of coalescence was observed in emulsions containing pectin. Emulsions stabilized by H-Mix were composed of smaller and well-defined droplets compared. Coincided with the microscopy results, H-Mix systems also had the lowest βcarotene release at the end of digestion (e.g., 6.4% release compared to 17% release in systems without pectin). Furthermore, when the emulsions contained higher protein β-carotene release decreased. We concluded that heating, biopolymer concentration, and protein concentration in the emulsions played the major roles in stabilizing the emulsion and βcarotene during digestion. Results can be applied to improve delivery properties of WPI-stabilized emulsions.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Tang, Y., & Vardhanabhuti, B. (2017, June). Effect of pectin on digestion properties and ?-carotene delivery of whey protein-stabilized emulsions. Presented at the American Dairy Science Association Annual Meeting, Pittsburge, PA.


Progress 11/23/15 to 09/30/16

Outputs
Target Audience:Food Industry, academia, and general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One Master student is working on the project. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?We will investigate the effect of heated protein-polysaccharide soluble aggregates on digestion properties of emulsions containng omega 3 or other bioactive oil.

Impacts
What was accomplished under these goals? . Our overall goal is to determine how various factors that affect protein and polysaccharide interactions influence the digestion of lipid micro- and nanoparticles as well as the release and delivery of functional lipids. In this period, we investigated the effect of pectin on digestion properties and encapsulation of β-carotene of whey protein-stabilized emulsion. Our results showed pectin led to a drastic change in degestion properties of protein-stabilized emulsions. Confocal images revealed that emulsions stabilized by unheated or heated whey protein had the highest degree of coalescence. Extensive flocculation but less degree of coalescence was observed in emulsions containing pectin. Emulsions stabilized by heated whey protein and pectin soluble aggregates were composed of smaller and well-defined droplets compared. Coincided with the microscopy results, emulsions stabilized by heated whey protein and pectin also had the lowest β-carotene release at the end of digestion.

Publications

  • Type: Other Status: Submitted Year Published: 2017 Citation: Tang, Y. and Vardhanabhuti, B. Effect of pectin on digestion properties and ?-carotene delivery of whey protein-stabilized emulsions. Abstract submitted to be considered for the presentation at the American Dairy Science Association Annual Meeting.