Progress 12/15/15 to 12/14/18
Outputs
Target Audience:Presented reasearch findings at departmental seminar series reaching fellow undergraduate, graduate, post-docotoral fellows. Presented Poster at WSU Research Showcase to the general public, undergraduate and graduate students, postdocs, and professors from a broad range of fields. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?2 manuscripts have been prepared and submitted What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
1. CspA interacting RNAs were identified and validated in vitro through development of a novel fluorescent RNA binding assay. 2. CspA Deletion strains were generated and found to result in delayed nodule maturation in symbiosis with alfalfa. Transcriptomic analysis of deletion strains under free-living and symbiotic conditions revealed altered levels of interacting sRNAs, in some cases located upstream of key stress responsive transcriptional regulators. 3. CspA family protein levels were found to have stress responsive expression as well as symbiotic developmental stage specific expression
Publications
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Progress 12/15/16 to 12/14/17
Outputs
Target Audience:Presented reasearch findings at departmental seminar series reaching fellow undergraduate, graduate, post-docotoral fellows. Presented Poster at WSU Research Showcase to the general public, undergraduate and graduate students, post-docs, and professors from a broad range of fields. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?2 manuscripts are under review at respected journals in the field. What do you plan to do during the next reporting period to accomplish the goals?Our goal for the next reporting peroid is to see the 2 manuscripts under review through to publication.
Impacts
What was accomplished under these goals?
1. CspA interacting RNAs were identified and validated in vitro through development of a novel fluorescent RNA binding assay. 2. CspA Deletion strains were generated and found to result in delayed nodule maturation in symbiosis with alfalfa. Transcriptomic analysis of deletion strains under free-living and symbiotic conditions revealed altered levels of interacting sRNAs, in some cases located upstream of key stress responsive transcriptional regulators. 3. CspA family protein levels were found to have stress responsive expression as well as symbiotic developmental stage specific expression
Publications
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Progress 12/15/15 to 12/14/16
Outputs
Target Audience:Mentored / trained an undergraduate student who presented his work to fellow agricultural students and scientists at a departmental seminar.Attended the EMBO RNA Structure Meets Function workshop in Stockhom Sweden and shared research findings with fellow RNA scientists, attended USDA AFRI ELI Project Directors meeting in Washington DC and shared research with fellow Project Directors, Presented reasearch findings at departmental seminar series reaching fellow undergraduate, graduate, post-docotoral fellows. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Attended EMBO RNA Structure Meets Function Workshop in Stockholm, Sweden. Attended USDA AFRI ELI Project directors meeting in Washingotn D.C.. How have the results been disseminated to communities of interest?Work was presented locally at Washington State University through a departmental seminar. Work was presented internationally through a poster presentation at ainternational workshop in Stockhom, Sweden What do you plan to do during the next reporting period to accomplish the goals?We plan to publish a manscript with our CspA expression and deletion phenotype data. We plan to publish a manuscript with our Identification of CspA RNA targets. We plan to further our investigation into CspA control of gene expression through identifiying significant changes at the transcript and protein level in our deletion mutants.
Impacts
What was accomplished under these goals?
1. We identified CspA interacting RNAs by immunoprecipitation of native GFP-tagged proteins followed by RNA sequencing. We identified novel interactios between CspAs and a unique sRNA family and confirmed these interactions in vitro. 2. We generated rhizobial CspA deletion strains and determined that a double mutantstrain is symbiotically less effective due to delayednodule maturation. 3. We indentified specific abiotic stresses that significantly change expression of CspAs under free-living conditions using a fluorescent reporter system.
Publications
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