Progress 10/01/15 to 09/30/20
Outputs
Target Audience:Scientists, graduate students, postdoc researchers, undergrads. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Within this period of time, we had provided training opportunities to 2 undergrads, 2 visiting scholars, 1 research scientist, and 2 graduate students. How have the results been disseminated to communities of interest?1) Fifteenpeer-reviewed papers were published. A total of 961 citations have been generated since 2015. 2) I gave 8 invited talks at different public institutions. 3) My students gave multiple poster presentations in the Annual conference of American Soceity of Plant Biologists. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
For goal 1: 1. We have identified a few hundred genes involved in low Pi responses in the veins. Ten of them have been localized and three of them are being functionally characterized using a series of approaches such as molecular, physiological, and biochemical methods. For goal 2: 1. Our manipulation of sink source in potato led to a lower number of potato but these potato tubers have larger sizes. These tuber initiation of these potatos is also ealier than the wild type controls. 2. Our manipulation of sink source in rice has not led to an altered phenotype. 3. Our manipulation of sink source in soybean has led to an increased seed number. More detailed characterization is undergoing. In summary, our studies demonstrated the importance of vascular tissues in abiotic stress physiology and yield improvement. A more specific focus on this tissue led a more accurate dissection on molecular pathways and promising genes that can be used for crop improvement. Our great success on the seed number increase (over 30%) in soybean with increased photosynthesis and improved phloem movement of carbon and nitrogen prove that, in addition to sink strength,source strength could also be a limiting factor in crops. This is a surprising result because most scientists in this field believe that source strength is normally not a limiting factor for yield.Our failure in rice using a similar approache proved that the vascular tissue specific manipulation may be species specific.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Xu, C., Xia, Z., Huang, Z., Xia, C., Huang, J., Zha, M., Wang, S., Imran, M., Casteel, S., Jiang, Y. and Zhang, C., 2019. Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress. Crop Breeding, Genetics and Genomics, 2(1).
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Sun, L., Pan, Y., Wu, J., Zhao, D., Hui, M., Zhu, S., Zhu, X., Li, D., Song, F. and Zhang, C., 2020. Based Analytical Devices for the Rapid and Direct Electrochemical Detection of Hydrogen Peroxide in Tomato Leaves Inoculated with Botrytis cinerea. Sensors, 20(19), p.5512.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Xia, C. and Zhang, C., 2020. Long-Distance Movement of mRNAs in Plants. Plants, 9(6), p.731.
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Xia, C., Huang, J., Lan, H. and Zhang, C., 2020. Long-Distance Movement of Mineral Deficiency-Responsive mRNAs in Nicotiana Benthamiana/Tomato Heterografts. Plants, 9(7), p.876.
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Progress 10/01/18 to 09/30/19
Outputs
Target Audience:Scientists, graduate students, postdoc researchers, undergrads. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We have trained a number of graduate students, undergraduate students, and visiting scholars. How have the results been disseminated to communities of interest?1) We published our discoveries in both peer reviewed jourals 2) We published our discoveries in books 3) We attended meetings to present our discoveries 4) We gave scientific presentations in other universities What do you plan to do during the next reporting period to accomplish the goals?1) Since we have identified a few candidate genes associated with thesystemic signaling process in response to low Pi, we will do more in-depth functional characterization work on these genes. Downstream physiological and agronomical analysis on plants with altered expression of these genes will be pursued. 2) We will start conducting small RNA and protein analysis in the vasculature tissues in Plantago major in response to low P. 3) We will develop a transformation protocol for Plantago major, a model species used for systemic signaling to mineral deficiencies. 4) We will study the agronomic improvements in potato and rice in growth chambers.
Impacts
What was accomplished under these goals?
1) We have identified a few hundred genes involved in the systemic signaling in plants grown under low P condition. Of which over ten genes have shown vascular specific localization. Our group are conducting functional characterization on these genes. 2) We are identifying more genes involved in the systemic signaling process in soybean plants in response to low P. 3) Our experimentsin the sink/source manipulation in potato and rice are not producing repeatable results. This is mainly due to the diseases our plants had when being grown in our greenhouse. We are repeating the experiments in growth chamber.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Chen, Qingshuai, Xiyu Xu, Di Xu, Haisen Zhang, Cankui Zhang, and Gang Li. "WRKY18 and WRKY53 coordinate with HISTONE ACETYLTRANSFERASE1 to regulate rapid responses to sugar." Plant physiology 180, no. 4 (2019): 2212-2226.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Liu, Yan, Dongfeng Ji, Robert Turgeon, Jine Chen, Tianbao Lin, Jing Huang, Jie Luo, Yan Zhu, Cankui Zhang, and Zhiqiang Lv. "Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress." International journal of molecular sciences 20, no. 10 (2019): 2486.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Lee, H., Chen, Z., Zhang, C. and Yoon, G. 2019. Editing of the OsACS locus alters phosphate deficiency-induced adaptive responses in rice seedlings. Journal of Experimental Botany 70, 1927-1940.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Yao, Y., Zhang, C., Camberato, J. and Jiang, Y. 2019. Nitrogen and carbon contents, nitrogen use efficiency, and antioxidant responses of perennial ryegrass accessions to nitrogen deficiency. Journal of Plant Nutrition 42: 2092-2101.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Ji, Z., Camberato, J., Zhang, C. and Jiang, Y. 2019. Effects of 6-Benzyladenine, ?-Aminobutyric acid, and nitric oxide on plant growth, chlorophyll fluorescence, and ion accumulation of perennial ryegrass cultivars to salinity stress. HortScience 54: 1418-1422
- Type:
Book Chapters
Status:
Published
Year Published:
2019
Citation:
Xia, C., Zheng, Y., Huang, J. , Fei, Z. and Zhang, C. 2019. Using heterografting system to identify long distance movement of mRNAs in response to mineral deficiencies. Methods in Molecular Biology 2014: 421-431.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2019
Citation:
Xu, C., Xia, Z., Huang, Z., Huang J., Xia C. and Zhang C. 2019 Physiological and molecular responses of reproductive stage soybean to heat stress Crop Breeding, Genetics and Genomics (submitted)
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Progress 10/01/17 to 09/30/18
Outputs
Target Audience:Scientists, graduate students, postdoc researchers, undergrads. Changes/Problems:1) Although we have produced transgenic potato and rice, we are not sure whether we can see improved agronomic traits. One reason is due to the low activity of promoters used in the sink tissue. Based on literature, we chose what could be used. However, we found the activity is not as high as the one described in literature. We will still continue this project for another year; if after more screening of the hundred transgenic plants we still cannot find a few ones with strong promoter activity, we may need to drop this project from our program. We will focus on the phosphorus work because we are making exciting progress. 2) Since Fe deficiency is another issue for agriculture, we are thinking to add this work in our program. The plant materials that we have been using for phosphorus study can also be used for Fe because systemic signaling is involved in both minerals. What opportunities for training and professional development has the project provided?One PhD student, one MS student, and two visiting scholars have been trained. How have the results been disseminated to communities of interest?Most of our results have been published as research articles. What do you plan to do during the next reporting period to accomplish the goals?1) Further dissect the molecular responses in soybean grown under low phosphorus conditions. 2) Continue our research on the transgenic rice and potato. 3) Continue the functional characterization on a few genes related to low phosphorus. 4) Submit 2-3 papers in these areas described above.
Impacts
What was accomplished under these goals?
1. Using Plantago major as a model species, we identified a few hundred genes from the phloem in response to low phosphorus conditions. We discovered that sugar metabolism, hormonal compound synthesis and phosphate remobilization are the major mechanisms used by plants to cope with low phosphorus stress conditions. These genes can be used to create crops that are more resistant to low phosphorus soils. 2. Using a tomato/tobacco heterografting system, we identified approximately 200 mRNAs that move from the leaves to the roots. Of which, over 100 are related to low phosphorus condition. Manipulation of these mobile mRNAs in the phloem could lead to crops with better phosphorus use efficiency. 3. Using Arabidopsis and soybean as model species, we have identified a few hundred genes that are responsive to low phosphorus growth condition. Ten of them have been found to be related to systemic signaling. We are studying their functions using molecular tools. 4. We have created transgenic potato and rice plants with increased sink and source strengths. We are still analyzing their physiological and agronomical alterations.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
1. Huang, J., Huang, Z., Zhou, X., Xia, C., Imran, M., Wang, S., ... & Zhang, C. (2018). Tissue-specific transcriptomic profiling of Plantago major provides insights for the involvement of vasculature in phosphate deficiency responses. Molecular Genetics and Genomics, 1-17.
2. Sui, X., Nie, J., Li, X., Scanlon, M. J., Zhang, C., Zheng, Y., ... & Zhang, Z. (2018). Transcriptomic and functional analysis of cucumber (Cucumis sativus L.) fruit phloem during early development. The Plant Journal.
3. Xu, C., Xia, C., Xia, Z., Zhou, X., Huang, J., Huang, Z., ... & Zhang, C. (2018). Physiological and transcriptomic responses of reproductive stage soybean to drought stress. Plant cell reports, 1-14.
4. Xia, C., Zheng, Y., Huang, J., Zhou, X., Li, R., Zha, M., ... & Zhang, C. (2018). Elucidation of the mechanisms of long-distance mRNA movement in a Nicotiana benthamiana/tomato heterograft system. Plant physiology, pp-01836.
5. Chen, Q., Payyavula, R. S., Chen, L., Zhang, J., Zhang, C., & Turgeon, R. (2018). FLOWERING LOCUS T mRNA is synthesized in specialized companion cells in Arabidopsis and Maryland Mammoth tobacco leaf veins. Proceedings of the National Academy of Sciences, 115(11), 2830-2835.
6. Zhang, C., & Turgeon, R. (2018). Mechanisms of phloem loading. Current opinion in plant biology, 43, 71-75.
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Progress 10/01/16 to 09/30/17
Outputs
Target Audience:Scientists, graduate students, postdoc researchers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?One PhD student and two visiting scientists are being trained when they performed these projects. How have the results been disseminated to communities of interest?One poster was presented during the 2017 American Soceity of Plant Biologists (ASPB) meeting in Hawaii. Three papers are being prepared based on our discoveries. What do you plan to do during the next reporting period to accomplish the goals?1) Dissect the translatomic alterations in the phloem companion cells of soybean under phosphate deficiency condition. 2) Phenotype transgenic potato for yield improvement. 3) Submit three papers in these two areas mentioned in 1 and 2.
Impacts
What was accomplished under these goals?
1. Using plantago major, we have identifed 196 mRNAs residing in the vasculature that are responsible to phosphate deficiency. Sugar metabolism and cross talk among different hormones are two of the major pathwas involved in phophate deficiency response in the vasculature of plantago major. 2. Using Arabidopsis as model, we have identifed over 100 mRNAs residing in the phloem companion cells responsible to phosphate deficiency. A number of transcription factors related to homornal synthesis and transport were discovered to be involved in early response to phosphate deficiency. 3. We have produced transgenic potato plants in which the movement of sucrose was increased. We are in the middle of assessment on yield improvement.
Publications
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience:Plant scientists and molecular biologists. Changes/Problems:A revised proposal has been submitted to the related office at Purdue University. What opportunities for training and professional development has the project provided?1. Trained graduate student Jing Huang on a series of techniques: growing plant in a hydroponic system; grafting; making RNA Seq libraries; sequencing analysis; etc. 2. Trained Research Scientist Xiangjun Zhou and graduate student Jing Huang on literature analysis and hypothesis formulation. 3. Graduate student Jing Huang is writing a paper on what we have obtained. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?1. Finish 1-2 manuscript on the hetero-grafting project. 2. Study where the origin of the mobile mRNAs in leaf. 3. Study whether proteins are also mobile from shoot to root under both normal and mineral deficiency conditions.
Impacts
What was accomplished under these goals?
For Aim 1, here is a list of accomplishments: 1. In our original Hatch proposal, we proposed to use tomato-potato hetero-graft system to study systemic signaling in response to P deficiency. Our experiment showed that the high genome similarity between the two species made the identification of authentic mobile mRNAs very difficult. Therefore, we develped another system (Benthamiana-tomato) to answer the same question. The more distant relationship between Benthamiana and tomato makes the identification of mRNAs easier than previous system. 2. We identified ca. 250 mobile mRNAs in the Benthamiana-tomato hetero-graft system under both normal and mineral deficiency conditions. In addition to P deficiency (proposed in our Hatch grant), we also included nitrogen and iron, two other important minerals, in our system. For Aim 2: we decided not to proceed with aim 2 because it is not in line with the major research interest in the Zhang lab. A revised proposal has been submitted to our related office at Purdue University.
Publications
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